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<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BM28</accession>
    <name>A0A4W2BM28_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BM28"/>
    <dbReference type="STRING" id="30522.A0A4W2BM28"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053965.1">
        <property type="protein sequence ID" value="ENSBIXP00000000031.1"/>
        <property type="gene ID" value="ENSBIXG00000030345.1"/>
    </dbReference>
    <dbReference type="OMA" id="CESIAYH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22520">
        <property type="entry name" value="KH-I_PCBP4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22523">
        <property type="entry name" value="KH-I_PCBP4_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="125"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="265"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="339"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="420" mass="43842" checksum="2C008A5326022104" modified="2019-09-18" version="1">MEFGRGAQGTWFGGEGRYLPHPVPESTIQDHQSVPRRRWPLPALAGPGEWGAGRFLARKQRRSPAACPIPAEQCPDHISEGSCPERITTITGSTAAVFHAVSMIAFKLDEDLCAAPANGGNVSRPPVTLRLVIPASQCGSLIGKAGTKIKEIRETTGAQVQVAGDLLPNSTERAVTVSGVPDAIILCVRQICAVILESPPKGATIPYHPSLSLGTVLLSTNQGFSVQGQYGTVTPAEVTKLQQLSGHAVPFASPSMVPGLDPSTQTSSQEFLVPNDLIGCVIGRQGSKISEIRQMSGAHIKIGNQAEGAGERHVTITGSPVSIALAQYLITACLETAKSTSGGTPGSAPTDLPAPFLATPDGPAHRSPRPAGHALCHLPLQLHRPQARTLLGSTTCLPRATAGLGGLHCQDGSGQWEQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BPC7</accession>
    <name>A0A4W2BPC7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="12">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FKBP6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FKBP6 family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027383834.1">
        <property type="nucleotide sequence ID" value="XM_027528033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BPC7"/>
    <dbReference type="STRING" id="30522.A0A4W2BPC7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015199.1">
        <property type="protein sequence ID" value="ENSBIXP00000000901.1"/>
        <property type="gene ID" value="ENSBIXG00000029229.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009922.1">
        <property type="protein sequence ID" value="ENSBIXP00005003637.1"/>
        <property type="gene ID" value="ENSBIXG00005009809.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883890"/>
    <dbReference type="CTD" id="8468"/>
    <dbReference type="GeneTree" id="ENSGT00940000158514"/>
    <dbReference type="OMA" id="CHRMFTP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045070">
        <property type="term" value="P:positive regulation of viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141196">
        <property type="term" value="P:transposable element silencing by piRNA-mediated DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000160">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000030">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042282">
        <property type="entry name" value="FKBP6/shu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46674">
        <property type="entry name" value="INACTIVE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46674:SF1">
        <property type="entry name" value="INACTIVE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="12" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="14">
        <location>
            <begin position="54"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="327" mass="37609" checksum="95BE4F3C5C27A34E" modified="2019-09-18" version="1">MGGSTRNPGVLQRDDSPSQSPYQRLSQRMLDISGDRGVLKDVIREGAGELVTPDASVLVKYSGYLEHMDKPFDSNCFRKTPRLMKLGEDITLWGMELGLLSMRRGELARFLFKPTYAYGTLGCPPLIPPNTTVLFEIELLDFLDSAESDKFCALSAEQQSQFPLQKVLKVAATEREFGNYLFRQNRFYDAKVRYKRALLLLHRRTAPPEEQHLVETAKLLVFLNLSFTYLKLERPTMALRYGEQALIIDRKNAKALFRCGQACLLMTEYQKARDFLVRAQREQPFNHDINNELKKLASYYRDYMDKEREMCHRMFASGDNGSTVGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BR90</accession>
    <name>A0A4W2BR90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RWD domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Protects DRG2 from proteolytic degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RWDD1/GIR2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BR90"/>
    <dbReference type="STRING" id="30522.A0A4W2BR90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013464.1">
        <property type="protein sequence ID" value="ENSBIXP00000001676.1"/>
        <property type="gene ID" value="ENSBIXG00000008228.1"/>
    </dbReference>
    <dbReference type="OMA" id="MVMIFTP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23816">
        <property type="entry name" value="RWD_RWDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000064">
        <property type="entry name" value="RWD domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="10"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="131" mass="14906" checksum="92D268B34D8ED75E" modified="2019-09-18" version="1">MTDYGEEQRNELEALESIYPDSFTVLSENPPSFTITVISEAGENDETVQTTLKFTYSEKYPDEAPLYEIFTQLNLENNDVADILKLLALQAEENLGIVDESLFQEMDDLELEEDDDDPDNNPADRESDLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTQ0</accession>
    <name>A0A4W2BTQ0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein TMEM103</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Involved in cell migration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the elongator complex which consists of ELP1, ELP2, ELP3, ELP4, ELP5 and ELP6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTQ0"/>
    <dbReference type="STRING" id="30522.A0A4W2BTQ0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009764.1">
        <property type="protein sequence ID" value="ENSBIXP00000003483.1"/>
        <property type="gene ID" value="ENSBIXG00000009901.1"/>
    </dbReference>
    <dbReference type="OMA" id="MFTELNS"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19495">
        <property type="entry name" value="Elp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001078">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09807">
        <property type="entry name" value="ELP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="266" mass="29554" checksum="381A012D4DA6F935" modified="2019-09-18" version="1">MFPELNNLLNTTPEGAEQGKLTLLCDAKTDGSFLVHHFLSFYLKANCKVCFVALVQSFSHYNMVGQKLGVSLTTARERGQLVVLEGLKSAVDIFFRPQEEPHPLQFLREANAGDLQPLYAFVRDALEPVDCGEAAWRCPVLLVDDLSVLLSLGVGPVAVLDFVHYCRATVCREWKGNVVALVHDSGDAEDEESDVLLNGLSHQSHLILRAEGLATGFCKDVHGQLRILWRRPLQPTTPRDRSLTYQYKIQDKSVSFFAKGMSPAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BXI5</accession>
    <name>A0A4W2BXI5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BXI5"/>
    <dbReference type="SMR" id="A0A4W2BXI5"/>
    <dbReference type="STRING" id="30522.A0A4W2BXI5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009108.1">
        <property type="protein sequence ID" value="ENSBIXP00000003814.1"/>
        <property type="gene ID" value="ENSBIXG00000010207.1"/>
    </dbReference>
    <dbReference type="OMA" id="CLESWEM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16450">
        <property type="entry name" value="mRING-C3HGC3_RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000598">
        <property type="entry name" value="E3 ubiquitin-protein ligase RFWD3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037381">
        <property type="entry name" value="RFWD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056527">
        <property type="entry name" value="WD40_RFWD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16047:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16047">
        <property type="entry name" value="RFWD3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23419">
        <property type="entry name" value="WD40_RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0853">WD repeat</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="306"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="159"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="790" mass="86872" checksum="8EFC4747FB6D4F08" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYX9</accession>
    <name>A0A4W2BYX9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Matrix metallopeptidase 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">MMP20</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027417927.1">
        <property type="nucleotide sequence ID" value="XM_027562126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYX9"/>
    <dbReference type="STRING" id="30522.A0A4W2BYX9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005393.1">
        <property type="protein sequence ID" value="ENSBIXP00000005604.1"/>
        <property type="gene ID" value="ENSBIXG00000011776.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020090.1">
        <property type="protein sequence ID" value="ENSBIXP00005011281.1"/>
        <property type="gene ID" value="ENSBIXG00005015765.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905142"/>
    <dbReference type="CTD" id="9313"/>
    <dbReference type="GeneTree" id="ENSGT00940000161277"/>
    <dbReference type="OMA" id="YKNPYGF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097186">
        <property type="term" value="P:amelogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022617">
        <property type="term" value="P:extracellular matrix disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF125">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610312" evidence="21">
        <location>
            <begin position="23"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="22">
        <location>
            <begin position="113"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="20">
        <location>
            <begin position="293"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="20">
        <location>
            <begin position="344"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="20">
        <location>
            <begin position="391"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="20">
        <location>
            <begin position="440"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="19">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="17">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="178"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C143</accession>
    <name>A0A4W2C143_BOBOX</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2353" mass="255303" checksum="16B812DC563F56B5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1A2</accession>
    <name>A0A4W2C1A2_BOBOX</name>
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        <submittedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2">Belongs to the plexin family.</text>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A4W2C1K0</accession>
    <name>A0A4W2C1K0_BOBOX</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
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    <name>A0A4W2C2J7_BOBOX</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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    </dbReference>
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    </evidence>
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</entry>
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    <accession>A0A4W2C2W5</accession>
    <name>A0A4W2C2W5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
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        <name evidence="15" type="primary">LOC113907027</name>
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            <taxon>Artiodactyla</taxon>
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    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C2W5"/>
    <dbReference type="STRING" id="30522.A0A4W2C2W5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004420.1">
        <property type="protein sequence ID" value="ENSBIXP00000006105.1"/>
        <property type="gene ID" value="ENSBIXG00000001144.1"/>
    </dbReference>
    <dbReference type="OMA" id="ELASCCY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15410">
        <property type="entry name" value="7tmA_OR5D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="311" mass="35241" checksum="3F9C2C71D22A0931" modified="2019-09-18" version="1">MDEKNCTAVTDFILLGFSDAPELRVFLFLLFLSIYGVTVWGNLGMIALIQVSSRLHTPMYFFLSHLSFVDFCYSTTITPKILANILNEDKAISFLECAVQFYLFCTFVVTEVILLAVMAYDRFVAISDPLLYMITMSRNLCVELVSCCYLNGVVCSLIHLCLALQIPSYRSNVINHFFCDLPPLLSLACSDVIVNQLVLYIVATFYEIITITVILMSYLFILITILRMHSADKRRKAFSTCASHLTVIIVFHGTILFIYCRPHSGNSMDTDKVATVFYTVVIPMLNPLIYSLRNKDVKEALRKVMSSKILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3G0</accession>
    <name>A0A4W2C3G0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC113885371</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3G0"/>
    <dbReference type="STRING" id="30522.A0A4W2C3G0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004052.1">
        <property type="protein sequence ID" value="ENSBIXP00000006286.1"/>
        <property type="gene ID" value="ENSBIXG00000001169.1"/>
    </dbReference>
    <dbReference type="OMA" id="RRVCICF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15945">
        <property type="entry name" value="7tmA_OR5C1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="34975" checksum="57B97B3A0AFA77E4" modified="2019-09-18" version="1">MLENNCTMPIEFLLVGFTDYLPLRVTLFLVFLIVYTLTMVGNVGLIILININSSLQTPMYYFLSNLSFLDISYSTAITPKMLVNFLASKKSISLSGCVLQMFFFGCFADAECLILAAMAYDRYAAICNPLLYSALVSQRVCVCCIVLAYFSGGVTSMVHVCLTFRLPFCGSNIVNHFFCDIPPLLALSCTDTYINELLLFALCGFIQTSTFVVIFISYFCILLTVLSIKSSGGRSKTFSTCASHLLAITLFYGTLLFMYLRPTTSYSPDTDKVVAVFYTVVFPMFNPIIYSFRNKDVKYALRKLLDRNGTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C4C6</accession>
    <name>A0A4W2C4C6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TBP-associated factor 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TAF8</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of the TFIID basal transcription factor complex, composed of TATA-box-binding protein TBP, and a number of TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379982.1">
        <property type="nucleotide sequence ID" value="XM_027524181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C4C6"/>
    <dbReference type="SMR" id="A0A4W2C4C6"/>
    <dbReference type="STRING" id="30522.A0A4W2C4C6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003845.1">
        <property type="protein sequence ID" value="ENSBIXP00000006400.1"/>
        <property type="gene ID" value="ENSBIXG00000001181.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011470.1">
        <property type="protein sequence ID" value="ENSBIXP00005002831.1"/>
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            <dbReference type="Ensembl" id="ENSBIXP00000006400.1"/>
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            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="310" mass="34227" checksum="834FF443A8089D28" modified="2019-09-18" version="1">MADAAATAGAAGSGTRSGSKQSTNPADNYHLARRRTLQVVVSSLLTEAGFESAEKASVETLTEMLQSYISEIGRSAKSYCEHTARTQPTLSDIVVTLVEMGFNVDTLPAYAKRSQRMVITAPPVTNQPVTPKALTAGQNRPHPPHIPSHFPEFPDPHTYIKTPTYREPVSDYQVLREKAASQRRDVERALTRFMAKTGETQSLFKDDVSTFPLIAARPFTIPYLTALLPSELEMQQMEETDSSEQDEQTDTENLPLHISPDDSGAEKENTSVLQQNPSLSGSRNGEESIIDNPYLRPVKKPKIRRKKSLS</sequence>
</entry>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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            <dbReference type="Pfam" id="PF24874"/>
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            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="349" mass="38637" checksum="1F4898A79FA69A18" modified="2019-09-18" version="1">KYPQPKGQKKKKIVKYGMGGLIILFLVAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTHHAYEELSKQFDPHPLAMQFISQYSPEDIVTVHIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGSVEYTNEKHTLDLAPNSTARRQLASLLEGTSDQSVVIPHLFPKYIRAPSGPEANPVKQLQPNEEADYLGVRIQLRRERVGTGAAGFLEWWVIELQDCQANCNLLPMVIFSDKHHGAVRVHRAGHRQVRARLLQRDLALHHVGAALRGPHPQAMPGHLPGAGDAGAGAGGGALRQAHLPVPLTRDHDQVDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7W8</accession>
    <name>A0A4W2C7W8_BOBOX</name>
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        <submittedName>
            <fullName evidence="13">3'-phosphoadenosine 5'-phosphosulfate synthase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PAPSS2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; sulfate assimilation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384935.1">
        <property type="nucleotide sequence ID" value="XM_027529134.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2C7W8"/>
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        <property type="gene ID" value="ENSBIXG00000013603.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005040484.1"/>
        <property type="gene ID" value="ENSBIXG00005007618.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000009613"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:adenylylsulfate kinase activity"/>
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    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:hormone metabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:sulfate assimilation"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000212">
        <property type="entry name" value="Adenylyl-sulfate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000001">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF16">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="10">
        <location>
            <begin position="41"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="12">
        <location>
            <begin position="223"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="11">
        <location>
            <begin position="389"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="620" mass="70217" checksum="755A1A9C646328F5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA16</accession>
    <name>A0A4W2CA16_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RNF166</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RING finger protein 166</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RING-type E3 ubiquitin transferase RNF166</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RNF166</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027369620.1">
        <property type="nucleotide sequence ID" value="XM_027513819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CA16"/>
    <dbReference type="SMR" id="A0A4W2CA16"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043626.1">
        <property type="protein sequence ID" value="ENSBIXP00000008435.1"/>
        <property type="gene ID" value="ENSBIXG00000014284.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007339.1">
        <property type="protein sequence ID" value="ENSBIXP00005004949.1"/>
        <property type="gene ID" value="ENSBIXG00005000607.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875428"/>
    <dbReference type="CTD" id="115992"/>
    <dbReference type="GeneTree" id="ENSGT00950000182909"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16549">
        <property type="entry name" value="RING-HC_RNF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034734">
        <property type="entry name" value="ZF_C2HC_RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18574">
        <property type="entry name" value="zf_C2HC_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51803">
        <property type="entry name" value="ZF_C2HC_RNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC RNF-type" evidence="16">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="237" mass="25981" checksum="4BE3AA79E50285F4" modified="2019-09-18" version="1">MAMFRSLVGSAQQRQPPGGPAGGDSALEAQYSCPICLEVYHRPVAIGSCGHTFCGECLQPCLQVPSPLCPLCRLPFDPKKVDKAAHVEKQLASYKAPCRGCSKKVTLAKMRVHVSSCMKVQEQMANCPKFVPVVPTSQPIPSAVPNRSTFACPYCGARNLDQQELVKHCVDNHRSDPNRVVCPICSAMPWGDPSYKSANFLQHLLHRHKFSYDTFVDYSIDEEAAFQAALALSLSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA81</accession>
    <name>A0A4W2CA81_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endothelial cell-specific molecule 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ESM1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027376148.1">
        <property type="nucleotide sequence ID" value="XM_027520347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CA81"/>
    <dbReference type="STRING" id="30522.A0A4W2CA81"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044303.1">
        <property type="protein sequence ID" value="ENSBIXP00000009760.1"/>
        <property type="gene ID" value="ENSBIXG00000015386.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005183.1">
        <property type="protein sequence ID" value="ENSBIXP00005006035.1"/>
        <property type="gene ID" value="ENSBIXG00005011585.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879093"/>
    <dbReference type="CTD" id="11082"/>
    <dbReference type="GeneTree" id="ENSGT00390000018810"/>
    <dbReference type="OMA" id="TAWSAKY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005171">
        <property type="term" value="F:hepatocyte growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902204">
        <property type="term" value="P:positive regulation of hepatocyte growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.20:FF:000002">
        <property type="entry name" value="Endothelial cell-specific molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038850">
        <property type="entry name" value="ESM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15428:SF0">
        <property type="entry name" value="ENDOTHELIAL CELL-SPECIFIC MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15428">
        <property type="entry name" value="ENDOTHELIAL CELL-SPECIFIC MOLECULE 1 ESM-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610414" description="Endothelial cell-specific molecule 1" evidence="10">
        <location>
            <begin position="22"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="11">
        <location>
            <begin position="24"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20323" checksum="B57FE5ABCB5412C1" modified="2019-09-18" version="1">MKRLLLLATLLVPAHLAAVWSRKYAVDCPERCDSSQCKSSSRCKRTVLDDCGCCRVCAAGLGETCYRTVSGMDGVKCGPGLRCQFYNEEDDFGDEFGICKDCPFGTFGMECKQICSCQSGICNRVTGKCLKFPFFQYSVAKATNQRFVSHTEHDVASGDGNAVREELVKENDAPSPVMKWLNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAG7</accession>
    <name>A0A4W2CAG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Core-binding factor subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polyomavirus enhancer-binding protein 2 beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">SL3-3 enhancer factor 1 subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">SL3/AKV core-binding factor beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CBFB</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer with RUNX1, RUNX2 and RUNX3. Interacts with COPRS. Found in a complex with PRMT5 and RUNX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CBF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372142.1">
        <property type="nucleotide sequence ID" value="XM_027516341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAG7"/>
    <dbReference type="STRING" id="30522.A0A4W2CAG7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018401.1">
        <property type="protein sequence ID" value="ENSBIXP00000009878.1"/>
        <property type="gene ID" value="ENSBIXG00000015492.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029177.1">
        <property type="protein sequence ID" value="ENSBIXP00005017366.1"/>
        <property type="gene ID" value="ENSBIXG00005003530.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876755"/>
    <dbReference type="CTD" id="865"/>
    <dbReference type="GeneTree" id="ENSGT00390000018132"/>
    <dbReference type="OMA" id="YAEISMV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016513">
        <property type="term" value="C:core-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048469">
        <property type="term" value="P:cell maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030098">
        <property type="term" value="P:lymphocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043371">
        <property type="term" value="P:negative regulation of CD4-positive, alpha-beta T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043378">
        <property type="term" value="P:positive regulation of CD8-positive, alpha-beta T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.250.10:FF:000001">
        <property type="entry name" value="Core-binding factor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.250.10">
        <property type="entry name" value="Core binding factor, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003417">
        <property type="entry name" value="CBF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036552">
        <property type="entry name" value="CBF_bsu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10276:SF3">
        <property type="entry name" value="CORE-BINDING FACTOR SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10276">
        <property type="entry name" value="CORE-BINDING FACTOR, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02312">
        <property type="entry name" value="CBF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50723">
        <property type="entry name" value="Core binding factor beta, CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="187" mass="21998" checksum="AD5B8BF5A2031604" modified="2019-09-18" version="1">MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDFEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSSLGGGDDLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAX7</accession>
    <name>A0A4W2CAX7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromosome 11 open reading frame 68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C29H11orf68</name>
        <name evidence="4" type="synonym">C11orf68</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0696 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027387658.1">
        <property type="nucleotide sequence ID" value="XM_027531857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAX7"/>
    <dbReference type="SMR" id="A0A4W2CAX7"/>
    <dbReference type="STRING" id="30522.A0A4W2CAX7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009812.1">
        <property type="protein sequence ID" value="ENSBIXP00000003456.1"/>
        <property type="gene ID" value="ENSBIXG00000009870.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022059.1">
        <property type="protein sequence ID" value="ENSBIXP00005012607.1"/>
        <property type="gene ID" value="ENSBIXG00005016817.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885967"/>
    <dbReference type="CTD" id="100125880"/>
    <dbReference type="GeneTree" id="ENSGT00390000011640"/>
    <dbReference type="OMA" id="WIAIYGP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000005">
        <property type="entry name" value="UPF0696 protein C11orf68 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015034">
        <property type="entry name" value="Bles03"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31977">
        <property type="entry name" value="UPF0696 PROTEIN C11ORF68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31977:SF1">
        <property type="entry name" value="UPF0696 PROTEIN C11ORF68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08939">
        <property type="entry name" value="Bles03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="292" mass="31403" checksum="99DA977B89ACC10B" modified="2019-09-18" version="1">MAAAAAAVAGAGRGGGGGAEPRQERSRARGWAGAERSEGRRMEPGEELEEEDSPGGREDGFTAEHLAAEAMAADMDPWLVFDARMTPATELDAWLAKYPPSQVTRYGDPGSPNSEPVGWIAAYGQGYIPNSGDVQGLQAAWEALQTSGRPVTPGTLRQLAITHQVLSGKWLIHLAPGFKLDHAWAGIARAVVEGRLQVAKVSPRAKEGGRQVICVYTDDFTDRLGVLEADAAIRAAGVKCLLTYKPDVYTYLGIYRANRWHLCPTLYESRFQLGGSARGSRVLDRANNVELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCW3</accession>
    <name>A0A4W2CCW3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 570</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF570</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCW3"/>
    <dbReference type="STRING" id="30522.A0A4W2CCW3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019708.1">
        <property type="protein sequence ID" value="ENSBIXP00000010719.1"/>
        <property type="gene ID" value="ENSBIXG00000002475.1"/>
    </dbReference>
    <dbReference type="OMA" id="CSGWESM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000944">
        <property type="entry name" value="zinc finger protein 232 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001255">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000238">
        <property type="entry name" value="Zinc finger protein 485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000826">
        <property type="entry name" value="Zinc finger protein 570"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002071">
        <property type="entry name" value="Zinc finger protein 570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="536" mass="62255" checksum="CFF4BC1F73CC6B28" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CH78</accession>
    <name>A0A4W2CH78_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cilia and flagella associated protein 58</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CFAP58</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027385024.1">
        <property type="nucleotide sequence ID" value="XM_027529223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CH78"/>
    <dbReference type="STRING" id="30522.A0A4W2CH78"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005297.1">
        <property type="protein sequence ID" value="ENSBIXP00000005657.1"/>
        <property type="gene ID" value="ENSBIXG00000011821.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018049.1">
        <property type="protein sequence ID" value="ENSBIXP00005009880.1"/>
        <property type="gene ID" value="ENSBIXG00005014662.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884578"/>
    <dbReference type="CTD" id="159686"/>
    <dbReference type="GeneTree" id="ENSGT00530000063534"/>
    <dbReference type="OMA" id="CQDDMRL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120229">
        <property type="term" value="P:protein localization to motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120317">
        <property type="term" value="P:sperm mitochondrial sheath assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049270">
        <property type="entry name" value="CFAP58_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF31">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21771">
        <property type="entry name" value="CFAP58_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 58 central coiled coil" evidence="4">
        <location>
            <begin position="366"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="848"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="106"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="784"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="873" mass="103738" checksum="13D33600631147F7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ63</accession>
    <name>A0A4W2CJ63_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ly6/PLAUR domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJ63"/>
    <dbReference type="STRING" id="30522.A0A4W2CJ63"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021621.1">
        <property type="protein sequence ID" value="ENSBIXP00000011965.1"/>
        <property type="gene ID" value="ENSBIXG00000017119.1"/>
    </dbReference>
    <dbReference type="OMA" id="FQHIYFG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23565">
        <property type="entry name" value="TFP_LU_ECD_LYPD5_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23566">
        <property type="entry name" value="TFP_LU_ECD_LYPD5_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000020">
        <property type="entry name" value="LY6/PLAUR domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018363">
        <property type="entry name" value="CD59_antigen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10624:SF9">
        <property type="entry name" value="LY6_PLAUR DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10624">
        <property type="entry name" value="UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00983">
        <property type="entry name" value="LY6_UPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021221865" description="Ly6/PLAUR domain-containing protein 5" evidence="9">
        <location>
            <begin position="20"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="10">
        <location>
            <begin position="54"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="10">
        <location>
            <begin position="140"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="266" mass="28359" checksum="E8B3FFC4516EF255" modified="2019-09-18" version="1">MAVGVTRVILLGLFGPVLCLTDVQTPESSGSQAQQCYSFQHIYFGPFDLSNVNFSNVSCPQGCSEAVLSLNTGYRSSVTLVQKGCWRGPLTGQMLSDDKSLPPDYSVVRRCATDFCNANLQTHDSLPNLSPAPNPPTLSGTECYACVGIHPEDCTPEKSRRVQCHQDQSVCFQGSGRMTLGNFSAPVYIRTCQRPSCTIKGTTSPWTNIDLQGSCCEGDLCNRDSLTQPFTSASDTAAPRVPHVTVLLLVVSLLGGALGGPLGLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLU4</accession>
    <name>A0A4W2CLU4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Class E basic helix-loop-helix protein 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Class B basic helix-loop-helix protein 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLU4"/>
    <dbReference type="SMR" id="A0A4W2CLU4"/>
    <dbReference type="STRING" id="30522.A0A4W2CLU4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022806.1">
        <property type="protein sequence ID" value="ENSBIXP00000012731.1"/>
        <property type="gene ID" value="ENSBIXG00000017782.1"/>
    </dbReference>
    <dbReference type="OMA" id="AGDIFHK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18954">
        <property type="entry name" value="bHLH_TS_bHLHe22_bHLHb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000026">
        <property type="entry name" value="Basic helix-loop-helix family, member e23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF52">
        <property type="entry name" value="CLASS E BASIC HELIX-LOOP-HELIX PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="222"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="130"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="361" mass="35334" checksum="A3F3FD64B6B46180" modified="2019-09-18" version="1">MERGLHLGAAAAGEDDLYLHKSLSASAAKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLGCFEPADPEGAGLLLPPPGGGGGAGGGGGGGVGVPGLLVGSAGVGGDPSLSSLPAGAALCLKYGESAGRGSVAESSGGEQSPDDDSDGRCELVLRPGGTDPRASPGAGGGGTKAAEGCSNALLHSGGGAPPGAPSSGGGGGSGGGGGGSGGSKKSKEQKALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQAISAASLPSSAAAAAAAAALHPALGAYEQAAGYPFSAGLPPAASCPEKCALFNSVSSSLCKQCTEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CM51</accession>
    <name>A0A4W2CM51_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ARVCF delta catenin family member</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ARVCF</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CM51"/>
    <dbReference type="STRING" id="30522.A0A4W2CM51"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046413.1">
        <property type="protein sequence ID" value="ENSBIXP00000014240.1"/>
        <property type="gene ID" value="ENSBIXG00000018974.1"/>
    </dbReference>
    <dbReference type="OMA" id="XFELLYQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000007">
        <property type="entry name" value="ARVCF, delta catenin family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF5">
        <property type="entry name" value="SPLICING REGULATOR ARVCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="527"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="787"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="267"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="673"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="825"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="958"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="986"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1012"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1028"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1070" mass="114157" checksum="5DA21AF7CBB6167B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPG9</accession>
    <name>A0A4W2CPG9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein</fullName>
            <shortName evidence="9">Cnt-b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centaurin-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ACAP3</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate protein binding to PIP2 or PIP3 containing membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CPG9"/>
    <dbReference type="STRING" id="30522.A0A4W2CPG9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046236.1">
        <property type="protein sequence ID" value="ENSBIXP00000013851.1"/>
        <property type="gene ID" value="ENSBIXG00000018711.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000913.1">
        <property type="protein sequence ID" value="ENSBIXP00005032720.1"/>
        <property type="gene ID" value="ENSBIXG00005001528.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156199"/>
    <dbReference type="OMA" id="GSVMSCE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13250">
        <property type="entry name" value="PH_ACAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000007">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000025">
        <property type="entry name" value="arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000020">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000026">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF407">
        <property type="entry name" value="ARF-GAP WITH COILED-COIL, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="1 9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="226"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="12">
        <location>
            <begin position="361"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="686"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="719"/>
            <end position="751"/>
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    <sequence length="819" mass="90995" checksum="406015942754433F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQW9</accession>
    <name>A0A4W2CQW9_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="19" type="primary">DDX24</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase that plays a role in various aspects of RNA metabolism including pre-mRNA splicing and is thereby involved in different biological processes such as cell cycle regulation or innate immunity. Plays an inhibitory role in TP53 transcriptional activity and subsequently in TP53 controlled cell growth arrest and senescence by inhibiting its EP300 mediated acetylation. Negatively regulates cytosolic RNA-mediated innate immune signaling at least in part by affecting RIPK1/IRF7 interactions. Alternatively, possesses antiviral activity by recognizing gammaherpesvirus transcripts in the context of lytic reactivation. Plays an essential role in cell cycle regulation in vascular smooth muscle cells by interacting with and regulating FANCA (Fanconi anemia complementation group A) mRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with FADD. Interacts with RIPK1; this interaction disrupts RLR signaling activation of IFN-dependent transcription factor IRF7. Interacts with NIP7. Interacts with EP300; this interaction prevents TP53 acetylation mediated by EP300.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.</text>
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        <property type="entry name" value="RNA HELICASE"/>
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        <property type="entry name" value="Helicase_C"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
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        <location>
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    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="801"/>
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    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="191"/>
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        </location>
    </feature>
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        <location>
            <begin position="61"/>
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    </feature>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="852" mass="94509" checksum="5D67599914B41510" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Artiodactyla</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="138"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="362" mass="41477" checksum="B0E37DB8FC303493" modified="2019-09-18" version="1">MLKILQARLQQYVNCELPNVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVAHIELWKILKEMGIPEHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNVGLEEAQAGIKIARRNINNLRYADDTTLMAESEEELKSLLMKVKESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSVFILGGSKITADGDCSHEIKRRLFLGRKVMTNLGSIFKGRDITVPTKVCLIKAMVFPVVMYGWESWTVKKAECRRTDAFELWCWRRLESPLDCKEIQPVHPKGDQSRVFIGRTDAKTETPILWPPCEELTHWKRPILGGTGGRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVR5</accession>
    <name>A0A4W2CVR5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CFAP157 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CVR5"/>
    <dbReference type="SMR" id="A0A4W2CVR5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027603.1">
        <property type="protein sequence ID" value="ENSBIXP00000015934.1"/>
        <property type="gene ID" value="ENSBIXG00000020269.1"/>
    </dbReference>
    <dbReference type="OMA" id="ARYQKKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038844">
        <property type="entry name" value="CFAP157"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31954">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31954:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="39"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="305"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="522" mass="60647" checksum="25F77BAC9B7CF4CC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVS9</accession>
    <name>A0A4W2CVS9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">60S ribosomal protein L37</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CVS9"/>
    <dbReference type="STRING" id="30522.A0A4W2CVS9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030048.1">
        <property type="protein sequence ID" value="ENSBIXP00000017508.1"/>
        <property type="gene ID" value="ENSBIXG00000021508.1"/>
    </dbReference>
    <dbReference type="OMA" id="NGFRENI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000001">
        <property type="entry name" value="Ribosomal protein L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF22">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="97" mass="10853" checksum="45F99DCEB38D8E49" modified="2019-09-18" version="1">MTKGTSSFGKHQNKTHTLCCCCGSKAYHLQKSTYGKCGYPAKQKRKYNWSGKAKRRNTTGTGRMQHLKSVYGRFRHGFHERTALKPQRAAVAVSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWM4</accession>
    <name>A0A4W2CWM4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SMC5</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">Forms a heterodimer with SMC6. Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. Interacts with NSMCE2. Interacts with SLF2; this interaction induces an association of the SLF1-SLF2 complex with the SMC5-SMC6 complex. Interacts with RAD18; this interaction is increased in a SLF1 or SLF2-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWM4"/>
    <dbReference type="STRING" id="30522.A0A4W2CWM4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028089.1">
        <property type="protein sequence ID" value="ENSBIXP00000016244.1"/>
        <property type="gene ID" value="ENSBIXG00000003763.1"/>
    </dbReference>
    <dbReference type="OMA" id="RFWTSQP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000793">
        <property type="entry name" value="Structural maintenance of chromosomes protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001434">
        <property type="entry name" value="Structural maintenance of chromosomes protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0779">Telomere</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="22">
        <location>
            <begin position="148"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="331"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="747"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="985"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1197" mass="138709" checksum="70ADA02E34215A1B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWT2</accession>
    <name>A0A4W2CWT2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heme-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein SOUL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HEBP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Monomer. Interacts with LRPPRC. May interact with BCL2L1; an interaction with BCL2L1 was observed using a peptide, but not with the full-length protein. The full-length protein would have to undergo a major conformation change for the interaction to occur. Interacts with PDCD6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWT2"/>
    <dbReference type="SMR" id="A0A4W2CWT2"/>
    <dbReference type="STRING" id="30522.A0A4W2CWT2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047448.1">
        <property type="protein sequence ID" value="ENSBIXP00000016380.1"/>
        <property type="gene ID" value="ENSBIXG00000020585.1"/>
    </dbReference>
    <dbReference type="OMA" id="YEIRTYH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000006">
        <property type="entry name" value="heme-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF70">
        <property type="entry name" value="HEME-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="205" mass="23029" checksum="A7982A0BC808A847" modified="2019-09-18" version="1">MAEVLEPDSRAAEGSEAPVVETPGWEVPEDAGPQPGSYEVRHYGPAKWVSTAVESMDWDSAMQTGFTRLKSYLQGKNEKEMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQSDPPRPAESDVFIEDRAEMTVFVRSFDGFSSAQKNQEQLLTLASILREEGKVFDEKVYYTAGYNSPFKLLDRNNEVWLIQKNEPSKERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CX60</accession>
    <name>A0A4W2CX60_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CX60"/>
    <dbReference type="STRING" id="30522.A0A4W2CX60"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047505.1">
        <property type="protein sequence ID" value="ENSBIXP00000016503.1"/>
        <property type="gene ID" value="ENSBIXG00000020672.1"/>
    </dbReference>
    <dbReference type="OMA" id="DPTQVIW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF24">
        <property type="entry name" value="CSC1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="610"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="695"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="53"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="227"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="421"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="803" mass="91737" checksum="69C68051D85FE0B8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYL0</accession>
    <name>A0A4W2CYL0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Diacylglycerol O-acyltransferase 2</fullName>
            <ecNumber evidence="12">2.3.1.20</ecNumber>
            <ecNumber evidence="11">2.3.1.76</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Acyl-CoA retinol O-fatty-acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Diglyceride acyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="39 40">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="39 40" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="39" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38219"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:38220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38163"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:75583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:38164"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38435"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:38436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:37915"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:37916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-O-(9Z-octadecenyl)-glycerol + (9Z)-octadecenoyl-CoA = 1-O-(9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:55340"/>
            <dbReference type="ChEBI" id="CHEBI:34116"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:197429"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:55341"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38439"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:38440"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:37911"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:37912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38071"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:38072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:11488"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:63410"/>
            <dbReference type="EC" id="2.3.1.76"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:11489"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl hexadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:38175"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:38176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:10869"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="12"/>
    <dbReference type="EC" id="2.3.1.76" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYL0"/>
    <dbReference type="STRING" id="30522.A0A4W2CYL0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020794.1">
        <property type="protein sequence ID" value="ENSBIXP00000011436.1"/>
        <property type="gene ID" value="ENSBIXG00000016676.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050252">
        <property type="term" value="F:retinol O-fatty-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046339">
        <property type="term" value="P:diacylglycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF14">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="24" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="21 35" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="23 37" id="KW-0472">Membrane</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <keyword evidence="17 37" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20 37" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="38">
        <location>
            <begin position="344"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="36">
        <location>
            <begin position="806"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="35">
        <location>
            <begin position="557"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="36">
        <location>
            <begin position="866"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="36">
        <location>
            <begin position="877"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="36">
        <location>
            <begin position="892"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00012977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00033044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00041149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00041468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1019" mass="114957" checksum="B8D3C1986059BD7E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0I7</accession>
    <name>A0A4W2D0I7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">E3 ubiquitin-protein ligase RNF183</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RNF183</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Acts as an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Triggers apoptosis in response to prolonged ER stress by mediating the polyubiquitination and subsequent proteasomal degradation of BCL2L1. May collaborate with FATE1 to restrain BIK protein levels thus regulating apoptotic signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with FATE1. Interacts with SEC16A. Interacts with BCL2L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Golgi apparatus</location>
            <location evidence="19">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D0I7"/>
    <dbReference type="STRING" id="30522.A0A4W2D0I7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032832.1">
        <property type="protein sequence ID" value="ENSBIXP00000019300.1"/>
        <property type="gene ID" value="ENSBIXG00000022949.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007188.1">
        <property type="protein sequence ID" value="ENSBIXP00005005032.1"/>
        <property type="gene ID" value="ENSBIXG00005010812.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162965"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902237">
        <property type="term" value="P:positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16556">
        <property type="entry name" value="RING-HC_RNF183-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000409">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase RNF183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="120"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00064632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00067422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="297" mass="33573" checksum="67DE248FFCAF5FDC" modified="2019-09-18" version="1">MLGFRARCWPHPRATLVPHLPTCRSGRRCRQVRSRGCRSGHPAAAGAACALPWRRAPGLVARRQSPKALWILERKRLEPPTFRGSLAYYPSAKEAQDVSCQTPAVSGLRMAEQQGREPECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHISLVTPTRRRLLCPLCRHPTVLASGQPVTDLPTDTAVLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDLGPEPASQAGQPQDVGPSTRPVPIRSRYSLRECFRNPHFRIFAYMMAVILCGTVLFIFSIFCTRRFFWGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0P8</accession>
    <name>A0A4W2D0P8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="10">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 10">Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D0P8"/>
    <dbReference type="SMR" id="A0A4W2D0P8"/>
    <dbReference type="STRING" id="30522.A0A4W2D0P8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045464.1">
        <property type="protein sequence ID" value="ENSBIXP00000012207.1"/>
        <property type="gene ID" value="ENSBIXG00000017300.1"/>
    </dbReference>
    <dbReference type="OMA" id="TQPNVYQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000066">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase SSU72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="194" mass="22530" checksum="9256A1858564BC2F" modified="2019-09-18" version="1">MSFSPLKVAVVCMSNMNRSMEAHSILRKKGFSVRSFGAGSRVRLPGTARNLPVVYDFSTTYEQMRKDLVRIDRERYTSNGILHILGRNERIKPRPERFQECRDRFDVIFTCEESVYDRVIEELWVREQETCQPVYVINVNMDDNTEDATLGSLIICELCERLQQAEDMEGSLAELLLAVERKTGRSFLHTVCFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D277</accession>
    <name>A0A4W2D277_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 22 23">Clusterin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CLU</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Antiparallel disulfide-linked heterodimer of an alpha chain and a beta chain. Self-associates and forms higher oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Chromaffin granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7 22">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 22 23">Belongs to the clusterin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D277"/>
    <dbReference type="SMR" id="A0A4W2D277"/>
    <dbReference type="STRING" id="30522.A0A4W2D277"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031296.1">
        <property type="protein sequence ID" value="ENSBIXP00000018320.1"/>
        <property type="gene ID" value="ENSBIXG00000022175.1"/>
    </dbReference>
    <dbReference type="OMA" id="QGSKYIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042583">
        <property type="term" value="C:chromaffin granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016016">
        <property type="entry name" value="Clusterin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000753">
        <property type="entry name" value="Clusterin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016015">
        <property type="entry name" value="Clusterin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033986">
        <property type="entry name" value="Clusterin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016014">
        <property type="entry name" value="Clusterin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970:SF1">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01093">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002368">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00035">
        <property type="entry name" value="CLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00030">
        <property type="entry name" value="CLb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00492">
        <property type="entry name" value="CLUSTERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00493">
        <property type="entry name" value="CLUSTERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19 22" id="KW-0143">Chaperone</keyword>
    <keyword evidence="24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 22" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 22" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021524349" description="Clusterin" evidence="22">
        <location>
            <begin position="20"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin N-terminal" evidence="25">
        <location>
            <begin position="17"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin C-terminal" evidence="26">
        <location>
            <begin position="222"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="37"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00010069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00020334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="439" mass="51114" checksum="36979481A61B02DE" modified="2019-09-18" version="1">MKTLLLLMGLLLSWESGWAISDKELQEMSTEGSKYVNKEIKNALKEVKQIKTQIEQTNEERKLLLSSLEEAKKKKEDALNDTRDSENKLKASQGVCNETMTALWEECKPCLKQTCMKFYARVCRSGSGLVGHQLEEFLNQSSPFYFWINGDRIDSLMENDREQSHVMDVMEDSFTRASSIMDELFQDRFFLRRPQDTQYYSPFSSFPRGSLFFNPKSRFARNVMPFPLLEPFNFHDVFQPFYDMIHQAQQAMDAHLQRTPYHFPTMEFTENNDRTVCKEIRHNSTGCLRMKDQCEKCQEILEVDCSASNPTQTLLRQQLNASLQLAEKFSRLYDQLLQSYQQKMLNTSALLKQLNEQFTWVSQLANLTQSDDQHYLQVFTVNSHNSDPSIPSGLTKVIVKLFNSFPITVTVPQEVSSPNFMENVAEKALQQYRRKSQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2F3</accession>
    <name>A0A4W2D2F3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2F3"/>
    <dbReference type="STRING" id="30522.A0A4W2D2F3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049201.1">
        <property type="protein sequence ID" value="ENSBIXP00000019981.1"/>
        <property type="gene ID" value="ENSBIXG00000003985.1"/>
    </dbReference>
    <dbReference type="OMA" id="ERCWEMV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12901">
        <property type="entry name" value="SPRY_PRY_FSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035742">
        <property type="entry name" value="SPRY/PRY_FSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF8">
        <property type="entry name" value="FSD1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COS" evidence="6">
        <location>
            <begin position="104"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="163"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="290"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="507" mass="56928" checksum="62372406A1920FD6" modified="2019-09-18" version="1">MDSQKEALQRIISTLANKDEIQNFIDTLNQTLKGVQENSSNILSELDEEFDSLYSILDEVKESMINSIKQEQARKSQELQSQLSQCNNALENSEELLEFATRSLDIKEPEEFSKAARQIKDRVTMASAFRLSLKPKVSDNMTHLMVDFSQERQILQTLKFLPVPKAPEIDPVECLVADNSVTVAWRMPEEDNKIDHFILEYRKTNFDGLPRVKDERCWEIVDNIKGTEYTLSGLKFDSKYMNFRVRACNKAVAGEYSDPVTLETKALNFNLDNSSSHLNLRVEDTCVEWDPTGGKGQDSKSKGKENKGSVHVTSLKKHSRSGTPSPKRTSVGSRPPAVRGSRDRFTGESYTVLGDTAIESGQHYWEVKAQKDCKSYSVGVAYKTLGKFDQLGKTNTSWCIHVNNWLQNTFAAKHNNKVKALDVAVPERIGVFCDFDGGQLSFYDANSKQLLYSFKAKFTQPVLPGFMVWCGGLSLSTGMQVPSAVRTLQKNENGMTGSTSSLNNITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2F5</accession>
    <name>A0A4W2D2F5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Prepronociceptin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PNOC</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the opioid neuropeptide precursor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027404838.1">
        <property type="nucleotide sequence ID" value="XM_027549037.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027404840.1">
        <property type="nucleotide sequence ID" value="XM_027549039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2F5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031717.1">
        <property type="protein sequence ID" value="ENSBIXP00000018384.1"/>
        <property type="gene ID" value="ENSBIXG00000022220.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053025.1">
        <property type="protein sequence ID" value="ENSBIXP00005044105.1"/>
        <property type="gene ID" value="ENSBIXG00005027879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896915"/>
    <dbReference type="CTD" id="5368"/>
    <dbReference type="GeneTree" id="ENSGT00950000183149"/>
    <dbReference type="OMA" id="DCLNCHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001515">
        <property type="term" value="F:opioid peptide activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002367">
        <property type="entry name" value="Nociceptin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006024">
        <property type="entry name" value="Opioid_neupept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438:SF2">
        <property type="entry name" value="PREPRONOCICEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438">
        <property type="entry name" value="PROENKEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01160">
        <property type="entry name" value="Opiods_neuropep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01028">
        <property type="entry name" value="OPIOIDPRCRSR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01031">
        <property type="entry name" value="ORPHNNPRCRSR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01252">
        <property type="entry name" value="OPIOIDS_PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="5" id="KW-0555">Opioid peptide</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610658" evidence="6">
        <location>
            <begin position="20"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="201" mass="23020" checksum="3795452F0C1C4F07" modified="2019-09-18" version="1">MKILFCDLLLLSLFSSVSSSCQKDCLVCREKLRPTLDSFSLEMCILECEEKAFTSPLWTPCTKVMARGSWQLSPADPDHVAAALDQPRASEMQHLKRMPRVRSLFQRQKRTEPGLEEVGEIEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGVRAIPKAACLQPQTCRLGIRTPPSPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2Q2</accession>
    <name>A0A4W2D2Q2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">sphingosine kinase</fullName>
            <ecNumber evidence="7">2.7.1.91</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SPHK2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3U5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032158.1">
        <property type="protein sequence ID" value="ENSBIXP00000018872.1"/>
        <property type="gene ID" value="ENSBIXG00000004400.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="397" mass="45040" checksum="650D0C14F9396A4C" modified="2019-09-18" version="1">MNFQLFKLVLQKAVEPETKLPTSAGSWKKQESSRKTSISALLTMPKPLTVWITINWKILKEMGIPDHLICLLRNLYAGQDATVRTGHGTTDWFQIGKGVRQGCILLHCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAENEEELKSLLTKVKEKSEKVGLKLNIEKTKIMASGPITSWEIDGETVETVSDLILGGSKITADSDCSHEIQRCLLLGRKVMTNLNSIFKSRDITLPTKVRLVKAMVFPVVMYGCKSWTVKKAECQRIDSFEMWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVMGREACRAAIHGVTKSQTLLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4J1</accession>
    <name>A0A4W2D4J1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF12</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4J1"/>
    <dbReference type="STRING" id="30522.A0A4W2D4J1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032617.1">
        <property type="protein sequence ID" value="ENSBIXP00000019143.1"/>
        <property type="gene ID" value="ENSBIXG00000004458.1"/>
    </dbReference>
    <dbReference type="OMA" id="VYSCPCC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000064">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="25"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="636" mass="69534" checksum="ABC1CCF6A96005EB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D637</accession>
    <name>A0A4W2D637_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D637"/>
    <dbReference type="STRING" id="30522.A0A4W2D637"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033425.1">
        <property type="protein sequence ID" value="ENSBIXP00000019702.1"/>
        <property type="gene ID" value="ENSBIXG00000023250.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF301">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="102" mass="11655" checksum="869BA3997346224E" modified="2019-09-18" version="1">MGVQVKTISPGDGMLEDGKKFDSSRDRNKPFKFVLGKQEVIRGWEEWVAQMCHRGIRRLQRHTVSLYGALPDVPLHSLCKHLPRLNVFCHPLCSSPFSSYVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6F9</accession>
    <name>A0A4W2D6F9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Required for meiotic nuclear division 5 homolog B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RMND5B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. Catalytic activity of the complex is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Identified in the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5. Within this complex, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9 and/or RANBP10 form the catalytic core, while GID4, MKLN1, ARMC8 and YPEL5 have ancillary roles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027402609.1">
        <property type="nucleotide sequence ID" value="XM_027546808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D6F9"/>
    <dbReference type="STRING" id="30522.A0A4W2D6F9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049969.1">
        <property type="protein sequence ID" value="ENSBIXP00000021439.1"/>
        <property type="gene ID" value="ENSBIXG00000003805.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037415.1">
        <property type="protein sequence ID" value="ENSBIXP00005022885.1"/>
        <property type="gene ID" value="ENSBIXG00005004382.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895513"/>
    <dbReference type="CTD" id="64777"/>
    <dbReference type="GeneTree" id="ENSGT00940000153203"/>
    <dbReference type="OMA" id="FEATNND"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16795">
        <property type="entry name" value="dRING_RMD5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000143">
        <property type="entry name" value="Regulator of gluconeogenesis Rmd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037681">
        <property type="entry name" value="RMD5B_dRING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF6">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN TRANSFERASE RMND5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CTLH" evidence="9">
        <location>
            <begin position="155"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RING-Gid-type" evidence="10">
        <location>
            <begin position="338"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="8">
        <location>
            <begin position="338"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="393" mass="44382" checksum="FEC8110B2A5987A7" modified="2019-09-18" version="1">MEQCASVEREVDKVLQKFLTYGQHCEQSLEELLHYVGQLRAELASTAVQGTPLSATLSLVMSQCCRKIKDTVQKLASDHKDIHSSVSRVGKAIDRNFDSEICGVVSDAVWDSREKQQQTLQMAILEHLYQQGMLSVAEELCQESTLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVSHRQRLLELNSSLEFKLHRLQFIRLLAGGPEKQLEALSYARHFQPFAHVHQREIQVMMGSLVYLQLGLEKSPYCHLLDNSHWAEICETFTRDACSLLGLSVESPLSVSFASGCVALPVLMNIKAVMEQRQCSGVWSHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNPPIKLICGHVISRDALNKLINGGKLKCPYCPMEQNPADGKRIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D724</accession>
    <name>A0A4W2D724_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D724"/>
    <dbReference type="STRING" id="30522.A0A4W2D724"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033961.1">
        <property type="protein sequence ID" value="ENSBIXP00000020056.1"/>
        <property type="gene ID" value="ENSBIXG00000023465.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000033962.1">
        <property type="protein sequence ID" value="ENSBIXP00000020057.1"/>
        <property type="gene ID" value="ENSBIXG00000023465.1"/>
    </dbReference>
    <dbReference type="OMA" id="QHMGHRA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF40">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 4-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610697" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="177" mass="19907" checksum="66B7F1B1BBAB2271" modified="2019-09-18" version="1">MGFRQLCCVVLCLLGIAVCADPGITQIPKYLVMGMTDKKSLTCEQHLGHNAMYWYKQSAQKPPELMFIHNYQELAGNENVPSRFWPECPDSSQCRLDLNALKPQDSAVYLCASSRDTALQRQFLPVHKPPGSRQEAVGAIQVLVWHFPSDHSPACRVTWHRGGEFLPRHQQCLLGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D763</accession>
    <name>A0A4W2D763_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MOB kinase activator 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MOB3A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027402907.1">
        <property type="nucleotide sequence ID" value="XM_027547106.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402908.1">
        <property type="nucleotide sequence ID" value="XM_027547107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D763"/>
    <dbReference type="SMR" id="A0A4W2D763"/>
    <dbReference type="STRING" id="30522.A0A4W2D763"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033969.1">
        <property type="protein sequence ID" value="ENSBIXP00000020061.1"/>
        <property type="gene ID" value="ENSBIXG00000002672.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021991.1">
        <property type="protein sequence ID" value="ENSBIXP00005012566.1"/>
        <property type="gene ID" value="ENSBIXG00005005730.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895656"/>
    <dbReference type="CTD" id="126308"/>
    <dbReference type="GeneTree" id="ENSGT01120000271863"/>
    <dbReference type="OMA" id="HMGSEAH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="217" mass="25410" checksum="6FD0655A8A2CE648" modified="2019-09-18" version="1">MSNPFLKQVFNKDKTFRPKRKFEPGTQRFELHKKAQASLNAGLDLKLAVQLPAGEELNDWVAVHVVDFFNRVNLIYGTISDGCTERSCPIMSGGPKYEYRWQDENKFRRPTALSAPRYMDLLMDWIEVQINNEDVFPTNVGTPFPKNFLQVVKKILSRLFRVFVHVYIHHFDRIAQLGSEAHVNTCYKHFYYFVTEFGLIDTKELEPLKEMTARMCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8D0</accession>
    <name>A0A4W2D8D0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor 5W2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113896676</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027404690.1">
        <property type="nucleotide sequence ID" value="XM_027548889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8D0"/>
    <dbReference type="STRING" id="30522.A0A4W2D8D0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037193.1">
        <property type="protein sequence ID" value="ENSBIXP00000022181.1"/>
        <property type="gene ID" value="ENSBIXG00000025105.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054313.1">
        <property type="protein sequence ID" value="ENSBIXP00005000393.1"/>
        <property type="gene ID" value="ENSBIXG00005030287.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896676"/>
    <dbReference type="GeneTree" id="ENSGT01140000282514"/>
    <dbReference type="OMA" id="FCIFANS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="308" mass="34977" checksum="4F00031CA9E86643" modified="2019-09-18" version="1">MDRDNCSSLTEFIFLGISENTENKVTLFTMVLLVYLINLLANLGMITLIRMDLQLHTPMYFFLSHLSFCDLCYSTATSPKMLVDLFAKNKSISFHGCALQFLVFCIFANSEYLLLAVMPCDQYKAISSPLLFAVSMSNRVCSLLVAGVYLVAMADALIHTTLAFRLCFCGSNEINHFFYDLPPLFLLSCSDIQVNELALFLVYGFIELSTISGVLVFYFHIILSVLKIHSVEGRFKAFSTCTSHLTAVTIFQGTIFFMYFRLSSSYSLDQDKMTLLFFTLVIPMLNPLIYSLRNKDVKQALEKIKLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8P8</accession>
    <name>A0A4W2D8P8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8P8"/>
    <dbReference type="STRING" id="30522.A0A4W2D8P8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026440.1">
        <property type="protein sequence ID" value="ENSBIXP00000015131.1"/>
        <property type="gene ID" value="ENSBIXG00000019691.1"/>
    </dbReference>
    <dbReference type="OMA" id="MEMDSKS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001038">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001201">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001441">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="306"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="404"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="747"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="954"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="770"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="971"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="996" mass="106794" checksum="E09029CB1EDA4813" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA54</accession>
    <name>A0A4W2DA54_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Basement membrane-specific heparan sulfate proteoglycan core protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">HSPG2</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Has anti-angiogenic properties that require binding of calcium ions for full activity.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Has a strong tendency to aggregate in dimers or stellate structures. Interacts with other basement membrane components such as laminin, prolargin and collagen type IV. Interacts with COL13A1. Interacts with FGFBP1. Interacts with VWA1. Interacts (via C-terminus) with ECM1 (via C-terminus). Interacts with SVEP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2DA54"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="Ig_Perlecan_like"/>
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        <property type="entry name" value="IgI_Perlecan_like"/>
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        <property type="entry name" value="Laminin subunit alpha 2"/>
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        <property type="entry name" value="laminin subunit alpha-2 isoform X1"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="match status" value="10"/>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
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        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF267">
        <property type="entry name" value="BASEMENT MEMBRANE-SPECIFIC HEPARAN SULFATE PROTEOGLYCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="5"/>
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    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DC22"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036673.1">
        <property type="protein sequence ID" value="ENSBIXP00000021831.1"/>
        <property type="gene ID" value="ENSBIXG00000005046.1"/>
    </dbReference>
    <dbReference type="OMA" id="PIIMSPR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15421">
        <property type="entry name" value="7tmA_OR2T-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="34764" checksum="2C4BB48A59F5E40D" modified="2019-09-18" version="1">MAKRNNISTTDFILLGLFPEFQYASLLVYLILLVYLIALTGNSILTFLIWLDVHLHTPMYFLLSQLSLIDLFYISSSVPKMVINHSLGKHSISFTGCGIQMFFCLSLGGAECLLLTFMSYDQFVAICNPLQYTVIMNSKVCLLIAVASWTGGALNSLIQTIYTMHFPTCGLKEINHFFCEMPAVLKLSCEDTSDYEMLVVVVTSIVLLVVPFSLIIASYTLIFLTVLRMNSVKGRKKALATCSSHLTVVSLFFGPNIFIYMTFTSSHSPEQDQALSLFSNILTPMLNPLIYSLRNKEVVAALMKLMGRCGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DED2</accession>
    <name>A0A4W2DED2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DED2"/>
    <dbReference type="SMR" id="A0A4W2DED2"/>
    <dbReference type="STRING" id="30522.A0A4W2DED2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040704.1">
        <property type="protein sequence ID" value="ENSBIXP00000024512.1"/>
        <property type="gene ID" value="ENSBIXG00000026647.1"/>
    </dbReference>
    <dbReference type="OMA" id="WVTAEVT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.40.10:FF:000021">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF57">
        <property type="entry name" value="DIHYDROPYRIMIDINASE-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="178"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="618"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="684" mass="73988" checksum="8757B83ECD75B9D0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFA5</accession>
    <name>A0A4W2DFA5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Family with sequence similarity 83 member C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FAM83C</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFA5"/>
    <dbReference type="STRING" id="30522.A0A4W2DFA5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047991.1">
        <property type="protein sequence ID" value="ENSBIXP00000017480.1"/>
        <property type="gene ID" value="ENSBIXG00000021482.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010406.1">
        <property type="protein sequence ID" value="ENSBIXP00005003391.1"/>
        <property type="gene ID" value="ENSBIXG00005009544.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160254"/>
    <dbReference type="OMA" id="DYMTSHV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000004">
        <property type="entry name" value="protein FAM83H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07894">
        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="5">
        <location>
            <begin position="29"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="331"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="595"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="746" mass="80957" checksum="E51F9DB2293BC571" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DK15</accession>
    <name>A0A4W2DK15_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tryptase</fullName>
            <ecNumber evidence="8">3.4.21.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PRSS22</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.</text>
            <dbReference type="EC" id="3.4.21.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.59" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DK15"/>
    <dbReference type="STRING" id="30522.A0A4W2DK15"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053014.1">
        <property type="protein sequence ID" value="ENSBIXP00000026604.1"/>
        <property type="gene ID" value="ENSBIXG00000029753.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="68"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="313"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="343" mass="37266" checksum="33E265695F8CC7F0" modified="2019-09-18" version="1">SAQLGEGRGRKGQAGRRPDRVEGRAVHRPRCAKRGRGAGRGIDSAQTLTDPLPPGPPACGKRLQLNRIVGGEDSSDAEWPWVVSIRKNGTHHCAGSLLTSRWVLTAAHCFKDNLDKPTQFSVLLGAWQLGNPGPRSQEVGITWAQPHPVYSWKEGSRADIALVRLERAIQFSERVLPICLPDSTVQLSPDTNCWIAGWGSVHDGVPLSHPQTLQKLKVPIIDSATCSRLYWRGAGQGAITEDMLCAGYLEGERDACLGDSGGPLMCQVEGTWLLAGVISWGEGCAQRNRPGVYISLAAHRSWVQRIVQGVQLRGRSQRGGPTGRRGRALGARRPRGAERRRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKA4</accession>
    <name>A0A4W2DKA4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKA4"/>
    <dbReference type="STRING" id="30522.A0A4W2DKA4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048898.1">
        <property type="protein sequence ID" value="ENSBIXP00000019334.1"/>
        <property type="gene ID" value="ENSBIXG00000002733.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF42">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="5">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="76" mass="8521" checksum="6CCB1B68E32A9A24" modified="2019-09-18" version="1">YLFWYVQHLNKAPQLLLKGLTADKKVEHEGFQATPIKRDSSFHLQKQAVQASDSAVYYCALSDTVREGCGGAEHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKB7</accession>
    <name>A0A4W2DKB7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKB7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032891.1">
        <property type="protein sequence ID" value="ENSBIXP00000019345.1"/>
        <property type="gene ID" value="ENSBIXG00000002724.1"/>
    </dbReference>
    <dbReference type="OMA" id="HSSLYID"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042605">
        <property type="term" value="F:peptide antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051006">
        <property type="entry name" value="TCR_variable_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343:SF13">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0391">Immunity</keyword>
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    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021329562" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="157" mass="17222" checksum="DDDF38668785E3D5" modified="2019-09-18" version="1">MDKSLSLLILWLQLDWVSSKQDVSQSPEVLNVREGDSMVLNCSYTDSALYFLQWFRQDPGKGLTSLLSVQANQKEQASGRITVSLDKSSRHGALYIATSQHSDSTTYLCAVRHSAQREPVPSTETADGAPTTSCLGAEETFSIKGFSTLTFLPDRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLD7</accession>
    <name>A0A4W2DLD7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lebercilin-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Leber congenital amaurosis 5-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCA5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLD7"/>
    <dbReference type="SMR" id="A0A4W2DLD7"/>
    <dbReference type="STRING" id="30522.A0A4W2DLD7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051894.1">
        <property type="protein sequence ID" value="ENSBIXP00000024934.1"/>
        <property type="gene ID" value="ENSBIXG00000027067.1"/>
    </dbReference>
    <dbReference type="OMA" id="MKHQETQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026188">
        <property type="entry name" value="Lebercilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028933">
        <property type="entry name" value="Lebercilin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16650">
        <property type="entry name" value="C21ORF13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16650:SF9">
        <property type="entry name" value="LEBERCILIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15619">
        <property type="entry name" value="Lebercilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lebercilin" evidence="7">
        <location>
            <begin position="140"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="670" mass="76371" checksum="43B7EFEA1C3077C5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRT6</accession>
    <name>A0A4W2DRT6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-set and transmembrane domain-containing protein 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VSTM2A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. Promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027396069.1">
        <property type="nucleotide sequence ID" value="XM_027540268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRT6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050117.1">
        <property type="protein sequence ID" value="ENSBIXP00000021728.1"/>
        <property type="gene ID" value="ENSBIXG00000024758.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891773"/>
    <dbReference type="CTD" id="222008"/>
    <dbReference type="OMA" id="EVAGAQX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090336">
        <property type="term" value="P:positive regulation of brown fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070352">
        <property type="term" value="P:positive regulation of white fat cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000530">
        <property type="entry name" value="V-set and transmembrane domain containing 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF23">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021223466" description="V-set and transmembrane domain-containing protein 2A" evidence="12">
        <location>
            <begin position="25"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="27"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="236" mass="25811" checksum="DCF53F0AC4F097C5" modified="2019-09-18" version="1">MMGIFLAYVGFVFFSVLYVQQGLSSQAKFTEFPRNVTATEGQNVEMSCAFQSGSASVYLEIQWWFLRGPEDLEPGADVAGAQAELLPDRDPDGDGTKISTVKVQGNDISHKLQISKVRKKDEGLYECRVTDANYGELQEHKAQAYLKVNANSHARRMQAFEASPMWLQDMKPRKNVSVAAPSSMHSSANQRVPSTSSPQEVAKIPKQSPQSGARIATSHGLSVLLLVCGFVKGALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSV1</accession>
    <name>A0A4W2DSV1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">OCA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSV1"/>
    <dbReference type="STRING" id="30522.A0A4W2DSV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007166.1">
        <property type="protein sequence ID" value="ENSBIXP00000029432.1"/>
        <property type="gene ID" value="ENSBIXG00000000755.1"/>
    </dbReference>
    <dbReference type="OMA" id="QHRSSGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070974">
        <property type="term" value="F:POU domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047571">
        <property type="entry name" value="OCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037655">
        <property type="entry name" value="POU2AF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28376:SF1">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28376">
        <property type="entry name" value="RGD1562914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17721">
        <property type="entry name" value="POU2AF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52003">
        <property type="entry name" value="OCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCA" evidence="5">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="285" mass="30519" checksum="652D5F159A2A167F" modified="2019-09-18" version="1">MESGDYSKRVYQGVRVKHTVKDLLAEKRSRQTSNSRFNGSVGSAQSPFGPVPGSPVMSGYYGVRRAFLSDPDFHNTKQFANDACTSSAAKPFACESSAGQGHAALLDPYFPEPYGDHRPTALTPNSGTLFSASALPPLLPPPFPSDPAHFALRDSWEQTVPEGLSQPDPVPADTLQSLAPSTGSLSQLESGGAAQHRSAGWGGPLTGVQSYSLHALEDLYHTQGYPTPAPYPFTSFMTMSNDPPPKVGPFSQDEGADTSALQDTSPWTKEDGSLAWGTYECRRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYV8</accession>
    <name>A0A4W2DYV8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYV8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015648.1">
        <property type="protein sequence ID" value="ENSBIXP00000031658.1"/>
        <property type="gene ID" value="ENSBIXG00000001588.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="186" mass="20015" checksum="80EAD4359BD3C8DD" modified="2019-09-18" version="1">LFMAPVRFEWAVKLSVLLPVSNYRGHLCLPLQLAKAFLQPSIHLNLTTPCPDPGLALKGEEAPRPSPSSFSDLALRPQASPSKFLTLLSQQLLSLRASPGLLSSLCLFPSAAPPPVKPIYIRCLPSSPSVSPPTHRFVVSSGPLNSTSSQVMTFLELNSRHVSGSRTHGHVFSVSLTVFPAPGLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0V7</accession>
    <name>A0A4W2E0V7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mRNA(cytosine(6666)) deaminase</fullName>
            <ecNumber evidence="4">3.5.4.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">mRNA(cytosine(6666)) deaminase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">APOBEC2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine(6666) in apoB mRNA + H2O + H(+) = uridine(6666) in apoB mRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21772"/>
            <dbReference type="Rhea" id="RHEA-COMP:13888"/>
            <dbReference type="Rhea" id="RHEA-COMP:13889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="3.5.4.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.36" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027380427.1">
        <property type="nucleotide sequence ID" value="XM_027524626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E0V7"/>
    <dbReference type="SMR" id="A0A4W2E0V7"/>
    <dbReference type="STRING" id="30522.A0A4W2E0V7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004110.1">
        <property type="protein sequence ID" value="ENSBIXP00000030410.1"/>
        <property type="gene ID" value="ENSBIXG00000012381.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010018.1">
        <property type="protein sequence ID" value="ENSBIXP00005029725.1"/>
        <property type="gene ID" value="ENSBIXG00005009717.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881582"/>
    <dbReference type="CTD" id="10930"/>
    <dbReference type="GeneTree" id="ENSGT00940000156616"/>
    <dbReference type="OMA" id="WEEPDIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044029">
        <property type="term" value="P:positive regulation of gene expression via chromosomal CpG island demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000031">
        <property type="entry name" value="Probable C-&gt;U-editing enzyme APOBEC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050610">
        <property type="entry name" value="APOBEC_Cyt_Deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857:SF4">
        <property type="entry name" value="C-U-EDITING ENZYME APOBEC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857">
        <property type="entry name" value="MRNA EDITING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18772">
        <property type="entry name" value="APOBEC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="13">
        <location>
            <begin position="64"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25962" checksum="54881038F34C504C" modified="2019-09-18" version="1">MAQKEEAAAAAEPASQNGEEVENLEDPEKLKELIELPPFEIVTGERLPAHYFKFQFRNVEYSSGRNKTFLCYVVEAQSKGGQVQASRGYLEDEHATNHAEEAFFNSIMPTFDPALRYMVTWYVSSSPCAACADRIVKTLNKTKNLRLLILVGRLFMWEEPEIQAALRKLKEAGCRLRIMKPQDFEYIWQNFVEQEEGESKAFEPWEDIQENFLYYEEKLADILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E118</accession>
    <name>A0A4W2E118_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fibroblast growth factor receptor substrate 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FRS3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E118"/>
    <dbReference type="SMR" id="A0A4W2E118"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003931.1">
        <property type="protein sequence ID" value="ENSBIXP00000030475.1"/>
        <property type="gene ID" value="ENSBIXG00000012466.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PTB_FRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast growth factor receptor substrate 2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038742">
        <property type="entry name" value="FRS2_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF39">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR SUBSTRATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="7">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="495" mass="54621" checksum="195B87705291D4AD" modified="2019-09-18" version="1">MGSCCSCLNRDSVSDNHPTKFKVTNVDDEGVELGSGVMELTQSELVLHLHRREAVRWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCSRAEEIFNLLQDLMQCNSINVMEEPVIITRNSHPAELDLPRAPQPPNALGYTVSSFSNGFPGCPGEGPRFSAPRRPSTSSLRHPSLGEESTHALIAPDEQSHTYVNTPAGEEEQRRSRHCLQPLPEGRAPFTPQARGPDQRDPQVFLQPGQVKFVLGPTPARRHMTKCQGLCPSLQDAPHHNNNNEGPSECPAQPKCTYENVSGGLRPGAGWRLSPEEPGWNGLAQRRAALLHYENLPSLPPVWESQAPQPGEEAGDDGDSRDGLTPSSNGFPEGEEDETPLQKPTSTRAALRSHGSFPVPLTRRRGSPRVFNFDFRRPGPEPPRQLNYIQVELKGWGGDRPKGPPNPSAPRAPVPTTHPTRSSDSYAVIDLKKTVAMSNLQRALPRDDGTARKTRHNSTDLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E612</accession>
    <name>A0A4W2E612_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904749</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E612"/>
    <dbReference type="STRING" id="30522.A0A4W2E612"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022070.1">
        <property type="protein sequence ID" value="ENSBIXP00000034398.1"/>
        <property type="gene ID" value="ENSBIXG00000017350.1"/>
    </dbReference>
    <dbReference type="OMA" id="CIFWFNA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF36">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="165"/>
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        <location>
            <begin position="196"/>
            <end position="218"/>
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        <location>
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        <location>
            <begin position="43"/>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
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            <dbReference type="PROSITE" id="PS50262"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034398.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="314" mass="34990" checksum="D9DA7C6CF1B56724" modified="2019-09-18" version="1">MPSFNQSIFHPTVFFLTGIPGFEIYHAWVFIPFCCLYVIAISGNGMILFVIITESSLHEPMYYFLSMLSFMDLGLCLSTLVTMMGIFWFNVREISFDACIGQMFFIHGFTLMESSVLLAMAFDRYIAICNPLRYATILTNSTIIKVGFAIVVRVTTVVVPLLLLLKRLSFCGSHVLHHSYCFHPDVMKLSCTDTKINSAFGLAIVISTAALDSVLILLSYVPIINSVLSIASPEERKKAFGTCVSHISAVAIFYIPMISLSLVHRFGKHAPPLVHTLIANVYLLIPPVMNPIIYSVKTKQIQKAMVKVALSKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBD0</accession>
    <name>A0A4W2EBD0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="16">Zinc finger protein 677</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
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        <property type="gene ID" value="ENSBIXG00000006003.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KRAB"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="8"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
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        </location>
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        <location>
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        </location>
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            <begin position="330"/>
            <end position="357"/>
        </location>
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        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
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        <location>
            <begin position="386"/>
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        </location>
    </feature>
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        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
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        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="62370" checksum="0C6A2A3B8809EADA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EC08</accession>
    <name>A0A4W2EC08_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EC08"/>
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        <property type="gene ID" value="ENSBIXG00000020550.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="406" mass="46611" checksum="9D215EB1FBF5B3B5" modified="2019-09-18" version="1">MPKNAQTTAQLHSSHMLVKVMLKILQARLQQYVNRELPYVQAGFRKGRETTDQIANIHWIMEKARQFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWSQIGKGVRLGCILSPCLFNLYAEYIMRNAGLEETQAGIKTAGRNINNLRYADDPTLMAESEEELKSLLTKVKDESEKVGLKLNIQKTKIMASGPITSWEIDGETVEIVSGFIFLGSRITADGDCSHEIKRCLLLGRKVMTNLDSIFKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEICPGISLEGMMLKLKLQDFGHLMQRVDSLEKTLMLGGIGGRRRRGQHRMRWLDGITGWMASLTRWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECA1</accession>
    <name>A0A4W2ECA1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECA1"/>
    <dbReference type="SMR" id="A0A4W2ECA1"/>
    <dbReference type="STRING" id="30522.A0A4W2ECA1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045594.1">
        <property type="protein sequence ID" value="ENSBIXP00000034524.1"/>
        <property type="gene ID" value="ENSBIXG00000017525.1"/>
    </dbReference>
    <dbReference type="OMA" id="HFHILSH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF67">
        <property type="entry name" value="OLFACTORY RECEPTOR 51V1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="49"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="320" mass="36447" checksum="17AD42ED2D6A068E" modified="2019-09-18" version="1">NYLRSRMIALVSPSTNSSSFLLTGFSGLEQQYPWISVPFSTIYAVVLLGNCLVLYVIWTEPSLHQPMFYFLAMLAITDLCMGLSTVNTVLGILLGLIQKISLDSCITQAYFIHGLSFMESSVLLTMAFDRYIAICNPLRYSSILTNSRIIKIGLTILGRSFFFITPPIICLKYFHYCRPHILSHSFCLHQDLLRLACSDIRFNSYYALMLVICTLLLDAVLILFSYVLILKSVLAIASREERHKSFQTCISHVCAVLVFYIPIISLTMVHRFGKHLSPVVHVLMGNIYILFPPLMNPIIYSVKIQQIRSRMLRLFSLKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECE7</accession>
    <name>A0A4W2ECE7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Serine and arginine rich splicing factor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SRSF4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027373993.1">
        <property type="nucleotide sequence ID" value="XM_027518192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECE7"/>
    <dbReference type="SMR" id="A0A4W2ECE7"/>
    <dbReference type="STRING" id="30522.A0A4W2ECE7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028555.1">
        <property type="protein sequence ID" value="ENSBIXP00000037182.1"/>
        <property type="gene ID" value="ENSBIXG00000020712.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006495.1">
        <property type="protein sequence ID" value="ENSBIXP00005005367.1"/>
        <property type="gene ID" value="ENSBIXG00005011082.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877752"/>
    <dbReference type="CTD" id="6429"/>
    <dbReference type="GeneTree" id="ENSGT00940000156213"/>
    <dbReference type="OMA" id="XGYGYRR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048025">
        <property type="term" value="P:negative regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12600">
        <property type="entry name" value="RRM2_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000028">
        <property type="entry name" value="Putative serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000190">
        <property type="entry name" value="serine/arginine-rich splicing factor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047190">
        <property type="entry name" value="RRM2_SRSF4/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF45">
        <property type="entry name" value="SERINE_ARGININE-RICH SPLICING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="104"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="169"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="493" mass="56037" checksum="3953274BF66A7C07" modified="2019-09-18" version="1">MPRVYIGRLSYQARERDVERFFKGYGKILEVDLKNGYGFVEFDDLRDADDAVYELNGKDLCGERVIVEHARGPRRDGSYGSGRSGYGYRRSGRDKYGPPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRKNEGVIEFVSYSDMKRALEKLDGTEVNGRKIRLVEDKPGSRRRRSYSRSRSHSRSRSRSRHSRKSRSRSGSSKSSHSKSRSRSRSGSRSRSKSRSRSQSRSRSKKEKSRSPSKDKSRSRSRSADKRRSKSKDPAEEKVQNSDRTGKSKSRSPSRHKSKSRSRSQERGAEEARGSASRSRSKEKSLRKSRSRSQGGSRSRSRSKSKDKRKGRKRSREESRSRSRSRSKSERSRKRGGKRDSKSGSGGGSGSSKKKKKDDADRSQSRSRSRSGSKERERAKSESGQREGRGEGEDAGANQETRSRSRSNSKSKPNLPSESRSRSKSASKTRSRSKSRSRSGSRSPSRSRSRSHSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFG7</accession>
    <name>A0A4W2EFG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase Sgk3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Serum/glucocorticoid-regulated kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC113904228</name>
        <name evidence="25" type="synonym">SGK3</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFG7"/>
    <dbReference type="STRING" id="30522.A0A4W2EFG7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023070.1">
        <property type="protein sequence ID" value="ENSBIXP00000034885.1"/>
        <property type="gene ID" value="ENSBIXG00000017925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028499.1">
        <property type="protein sequence ID" value="ENSBIXP00005038411.1"/>
        <property type="gene ID" value="ENSBIXG00005020529.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153776"/>
    <dbReference type="OMA" id="FINGGEX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001240">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06870">
        <property type="entry name" value="PX_CISK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05604">
        <property type="entry name" value="STKc_SGK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000065">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000018">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000030">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037900">
        <property type="entry name" value="CISK_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037709">
        <property type="entry name" value="SGK3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PX" evidence="22">
        <location>
            <begin position="12"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="162"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="420"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="496" mass="57193" checksum="9635939F73F09C8E" modified="2019-09-18" version="1">TQRDYTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRRYAEFDKLYNTLRKQFPAMALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRHPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSTQKLLSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSIGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLNLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWTDLVQKKIPPPFNPNVVGPDDIRNFDAVFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFN7</accession>
    <name>A0A4W2EFN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Extracellular sulfatase</fullName>
            <ecNumber evidence="12">3.1.6.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SULF1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate.</text>
            <dbReference type="EC" id="3.1.6.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an aryl sulfate + H2O = a phenol + sulfate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140317"/>
            <dbReference type="EC" id="3.1.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus</location>
            <location evidence="1 12">Golgi stack</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell surface</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023451.1">
        <property type="protein sequence ID" value="ENSBIXP00000034996.1"/>
        <property type="gene ID" value="ENSBIXG00000018145.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005539">
        <property type="term" value="F:glycosaminoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008449">
        <property type="term" value="F:N-acetylglucosamine-6-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002063">
        <property type="term" value="P:chondrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048706">
        <property type="term" value="P:embryonic skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014846">
        <property type="term" value="P:esophagus smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035860">
        <property type="term" value="P:glial cell-derived neurotrophic factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032836">
        <property type="term" value="P:glomerular basement membrane development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030201">
        <property type="term" value="P:heparan sulfate proteoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060384">
        <property type="term" value="P:innervation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010575">
        <property type="term" value="P:positive regulation of vascular endothelial growth factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16147">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000003">
        <property type="entry name" value="Extracellular sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014615">
        <property type="entry name" value="Extracellular_sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024609">
        <property type="entry name" value="Extracellular_sulfatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF1">
        <property type="entry name" value="EXTRACELLULAR SULFATASE SULF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12548">
        <property type="entry name" value="DUF3740"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036665">
        <property type="entry name" value="Sulf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021524156" description="Extracellular sulfatase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="18">
        <location>
            <begin position="43"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Extracellular sulfatase C-terminal" evidence="19">
        <location>
            <begin position="533"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="Extracellular sulfatase C-terminal" evidence="19">
        <location>
            <begin position="680"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="573"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="641"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via 3-oxoalanine" evidence="15">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="3-oxoalanine (Cys)" evidence="13">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036665-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036665-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036665-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="868" mass="100442" checksum="8DCB16CBCF9EAC17" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFY2</accession>
    <name>A0A4W2EFY2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFY2"/>
    <dbReference type="STRING" id="30522.A0A4W2EFY2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046030.1">
        <property type="protein sequence ID" value="ENSBIXP00000035175.1"/>
        <property type="gene ID" value="ENSBIXG00000018350.1"/>
    </dbReference>
    <dbReference type="OMA" id="IISAMAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15413">
        <property type="entry name" value="7tmA_OR8K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="313" mass="35393" checksum="F8A8052796D715E5" modified="2019-09-18" version="1">MDKQNQTVPGEFILMRITDRPELQAAFFVLFLTIYAVSAVGNLGMVVLTKVDSKLQTPMYFFLRNLAFIDLGYSTSVGPKMLVAFVTDQNTISYHWCATQLTFFILFIISELFILSAMAYDRFVAICNPLLYTVVMSPKVCWVLVTVPYVYSASVSLITTIQIFISSFCGYNIISHFYCDSLPLLTLLCSSTWEIELFILICSVFNLVSSFLIVLVSYILILKAILKMNTAQGRQKAFSTCGSHLTVVAVLYATLAFMNMQPRSSHSFDTDKMASVFFTLVIPMLNPMIYSLRNKEVKGALHRMWENLCKIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFZ1</accession>
    <name>A0A4W2EFZ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFZ1"/>
    <dbReference type="STRING" id="30522.A0A4W2EFZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024049.1">
        <property type="protein sequence ID" value="ENSBIXP00000035193.1"/>
        <property type="gene ID" value="ENSBIXG00000018410.1"/>
    </dbReference>
    <dbReference type="OMA" id="NAYECKQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000666">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000218">
        <property type="entry name" value="Zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001157">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="350" mass="40552" checksum="2EB56E25721BB026" modified="2019-09-18" version="1">MLVEDEGVLTSEGFCEFSKHRKASTQKLNLIHQKVHTEEGPYECSQCGKFFSHRFRFLAHQRVRCVARLYDCNECGKSFSRRKNLTAHRKIHSGENAYECKQCNKSFTQKFNLIEHQRVHTGEKPYQCSQCGKSFAHKSSCLAHQRVHTGERPYECSECGKSLTNRSSLSYHLRLHTGEKPYECNECGKCFTTRSMLCNHQRVHSGERPFECSECGKFFSRNEQLSDHKNVHTGEKPYECNKCEKPFTSSSSLRHHQRVHTGEKSYECNKCWKSFTGSSSLRYHRRVHTGERPYKCSECGKSFAARTGLRHHERVHSGERPYECCQCGKLFSGISSLHYHCRVHSGEKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGV3</accession>
    <name>A0A4W2EGV3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGV3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027614.1">
        <property type="protein sequence ID" value="ENSBIXP00000036723.1"/>
        <property type="gene ID" value="ENSBIXG00000020210.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTHIYMA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="398" mass="45849" checksum="2A33B7A3788C9D7B" modified="2019-09-18" version="1">MNFQMFKAGFRKARGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNQLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEETQAGFKIAGRNLNNLSYADDTTLMAENEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRHLLLGRKVMTNLDSILKSRDITLPTKVHLVKAMVFPAVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGCSVEGLMLKLKLQYFGHLMQRVDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDLSLGELWEFVMDREAWRAAIHGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGW0</accession>
    <name>A0A4W2EGW0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGW0"/>
    <dbReference type="STRING" id="30522.A0A4W2EGW0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000722.1">
        <property type="protein sequence ID" value="ENSBIXP00000031556.1"/>
        <property type="gene ID" value="ENSBIXG00000013832.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048993.1">
        <property type="protein sequence ID" value="ENSBIXP00005017953.1"/>
        <property type="gene ID" value="ENSBIXG00005021396.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154319"/>
    <dbReference type="OMA" id="QEVPMNS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000239">
        <property type="entry name" value="Small ubiquitin-related modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="80" mass="9273" checksum="E9553AACE87A52CF" modified="2019-09-18" version="1">MSDQEAKPSTEDLGDKKEGEYSKLKVTRQDSSESHFKVKMVIHLKKLKESDCQRQEVPMNSLRFLLEGQKIVDNHTPKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EI84</accession>
    <name>A0A4W2EI84_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleoporin 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NUP43</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EI84"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029551.1">
        <property type="protein sequence ID" value="ENSBIXP00000037617.1"/>
        <property type="gene ID" value="ENSBIXG00000021219.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031029.1">
        <property type="protein sequence ID" value="ENSBIXP00005039534.1"/>
        <property type="gene ID" value="ENSBIXG00005003703.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004803"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000810">
        <property type="entry name" value="Nucleoporin 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652:SF0">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="284" mass="31339" checksum="809438D931B5CEF5" modified="2019-09-18" version="1">MEEIYAKFVSQKISKTRWRPLPPGSLQTAETFATGSWDNEENYVSMWSIGDFGNLDSDGGFEGDHQLLCSIKHHGDVMDLQFLDQERIVAASSTGCVTIFLHHPNNQTLSASQQWTAAHYHTGPGSPSCSNAPCTGVVCNNPEIVTVGEDGRINLFRADHKEAVRTIVWEVHFHPSNPDHLFTCSEDGSLWHWDASTDGPEKSSLFHQGGRNSTFLSHTISNQANVHQSLISSWLSTDPAKDRIEITSLLPNRTLSVNSLDVLGPCLVCGTDAEAIYVTRQLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIB5</accession>
    <name>A0A4W2EIB5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EIB5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017508.1">
        <property type="protein sequence ID" value="ENSBIXP00000032444.1"/>
        <property type="gene ID" value="ENSBIXG00000006577.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000711">
        <property type="term" value="P:meiotic DNA repair synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051445">
        <property type="term" value="P:regulation of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033536">
        <property type="entry name" value="Spata22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35258">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35258:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="364" mass="41472" checksum="3D2FB59AD95F7623" modified="2019-09-18" version="1">MKRNLNENSTRSTAGCLPVPLFNQKKRNRQPLTSNPCTNDPGISNASDNYDFPPLPSDWAWEAVNPELPPLTKTVNTGQIPHSVSNSLRSQDSLSKPIQSNAGSSKSDWSYRDGNKNTSLKTWDKNDFKPQCKRRNLMTNNGINSGPINLGAQQQKQLRVSESTNLPNQRESEALRQTKSSEIPGSTMRSLDKNSTLQAFKPNFQQNQFKKKMLDGFQEVNTLKKEASSYQLKLREKDNSLRIISAVIESMKYWREHVQKTVLLFEILAVLDSAVRPGPYCSKTFLMRDGKHTLPCVFYEIDRELPRLIRGRVHRCVGNYDQKKNIFKCVSVRPASASEQKTFQAFVKIVDAEMRYYTSVMNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EL91</accession>
    <name>A0A4W2EL91_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EL91"/>
    <dbReference type="STRING" id="30522.A0A4W2EL91"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026387.1">
        <property type="protein sequence ID" value="ENSBIXP00000036233.1"/>
        <property type="gene ID" value="ENSBIXG00000003521.1"/>
    </dbReference>
    <dbReference type="OMA" id="QHCAARS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040373">
        <property type="entry name" value="CASZ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451">
        <property type="entry name" value="TRANSCRIPTION FACTOR CASTOR PROTEIN MING -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN CASTOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="304"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="419" mass="45576" checksum="4803EC8FDFE5FDE2" modified="2019-09-18" version="1">MDYTGCSPGAMSSESSTMDRSCSSTPVGNESTAAGEQAARTGLGAKKRFWIIEDMSPFGKRRKTASSRKMLDEGMMLEGFRRFDLYEDCKDAACQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKASLSCHFADCPFSGSSTHFHCLRCRFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCAVPDCKYKLKCSHFHCTYPGCRHTVVGMSQMDSHKRKHEKQERGEPPAASPGPRASRPPAAATLLFLPGPALGPSDDPGPPDAPGPRVGPAAPGPAAGESSQEDDEEELELNEEADDDDDDEEEDDDEDDDEDDEDLRTDSEESLPEAPAVAAGPGSRSPELAALGAPAPPGPRPPPPPRSLPVAARRVARRGGRARAAPSGSCRFILLLLLLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EN09</accession>
    <name>A0A4W2EN09_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EN09"/>
    <dbReference type="STRING" id="30522.A0A4W2EN09"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031232.1">
        <property type="protein sequence ID" value="ENSBIXP00000038341.1"/>
        <property type="gene ID" value="ENSBIXG00000002850.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF120">
        <property type="entry name" value="ZINC TRANSPORTER ZIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="309" mass="32633" checksum="F3E02F5AFDC308AB" modified="2019-09-18" version="1">MEPLLGTKIGCLFALLVLTLVCGLIPICFKWFQTTTATGCHRRVLSFLGCTSAGVFLGAGFMHMTAEALEGIKSEIQNLVIQNRTKSEGHSDDDADSAYMEYPYGELIISLGFFLVFLLESLALQCCPGTAETPKVQEQELGTAHELAPHSHGPLSSPSGGPFRALILLLSLSFHSVFEGLAVGLQLTVASTVQLCLAVLAHKGIVVFGVGLRLVQVGTKSRWAVLSILSLALMSPLGLAIGLAVPQGDSKAGQGLAQSVLEGMAAGTFLYVTFLEILPRELASPEAPLAKWSCVAAGFAFMAVIALWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQ65</accession>
    <name>A0A4W2EQ65_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EQ65"/>
    <dbReference type="STRING" id="30522.A0A4W2EQ65"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032880.1">
        <property type="protein sequence ID" value="ENSBIXP00000039046.1"/>
        <property type="gene ID" value="ENSBIXG00000022972.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="P:antigen processing and presentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051944">
        <property type="entry name" value="BEACH_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108:SF3">
        <property type="entry name" value="WD REPEAT- AND FYVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="323" mass="34570" checksum="DA8EA76313E68A80" modified="2019-09-18" version="1">MYLFSLGSESPKGAIGHIVPTEKTILAVETNKMLLPPHWSRTFSWGFDDFSCCLGSYGSDKILVTFENLAAWGRCLCAACPSPTTIITAGTSAVVCVWELSMTKGRATGLRLKQALYGHTQAVTCLAASVTFSLLVSGSQDRTCILWDLDHLTHVARLPAHRDGISAVAISDVSGTIVSCAGAHLSLWDVNGQPLASITTAWGPEGAITCCYVVEGPAWDTSHVIITGSRDGMVRIWKTEDVKMSVPGQAAPEEPWAPSSPSPRVHRWEKNLALCRELDVSVALTGKPSKANSAVTALAVSRNQTKLLVGDEKGRIFCWSAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQW8</accession>
    <name>A0A4W2EQW8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Solute carrier family 25 member 24</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EQW8"/>
    <dbReference type="STRING" id="30522.A0A4W2EQW8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033587.1">
        <property type="protein sequence ID" value="ENSBIXP00000039366.1"/>
        <property type="gene ID" value="ENSBIXG00000004604.1"/>
    </dbReference>
    <dbReference type="OMA" id="RTHMQAS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000003">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="53"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="148"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37555" checksum="D3EEF838E316297E" modified="2019-09-18" version="1">MTVDWDEWKNYFLLHPAKNIDEIAHFWKRSTMIDIGESIAIPDDITEQEKRSGNWWKRLVAGGIAGGVARTCTAPFDRLKVMMQVIHSLPSGKMRLLDGFKQMVKEGGIISLWRGNGVNVLKIAPETALKVGTYEQYKKWLSSDGAKIGIIERFISGSLAGATAQTCIYPMEVIKTRLAVGKTGQYSGIIDCGKQLLKQEGARTFFKGYIPNLLGIIPYAGIDLCVYEHLKNRWLEHHARGSLDPGIAILLGCSTLSNACGQMASFPLNLIRTRMQAQALEEKGTTSMIQLIQDIYNKEGKRGFFRGVTPNIIKVLPSVCISCVTFEKVKGHVGFTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ET70</accession>
    <name>A0A4W2ET70_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Phosphatidylinositol phosphatase PTPRQ</fullName>
            <ecNumber evidence="6">3.1.3.67</ecNumber>
            <ecNumber evidence="5">3.1.3.86</ecNumber>
            <ecNumber evidence="4">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Receptor-type tyrosine-protein phosphatase Q</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">PTPRQ</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25528"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="EC" id="3.1.3.86"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:25529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:25018"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:32956"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:39019"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="EC" id="3.1.3.95"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:39020"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with TPRN. TPRN, CLIC5 and PTPQR form concentric rings at the base of stereocilia and may form a complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Basal cell membrane</location>
            <topology evidence="24">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Stereocilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.67" evidence="6"/>
    <dbReference type="EC" id="3.1.3.86" evidence="5"/>
    <dbReference type="EC" id="3.1.3.95" evidence="4"/>
    <dbReference type="STRING" id="30522.A0A4W2ET70"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035522.1">
        <property type="protein sequence ID" value="ENSBIXP00000040186.1"/>
        <property type="gene ID" value="ENSBIXG00000006233.1"/>
    </dbReference>
    <dbReference type="OMA" id="YYTVYIW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="CDD" id="cd14616">
        <property type="entry name" value="R-PTPc-Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000937">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001431">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000041">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001217">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001685">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001147">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000478">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000869">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001266">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001474">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001645">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001879">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002575">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL PHOSPHATASE PTPRQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
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    </dbReference>
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        <property type="match status" value="20"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
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    <keyword evidence="15 28" id="KW-0472">Membrane</keyword>
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    <keyword evidence="12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="2988"/>
            <end position="3010"/>
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        <location>
            <begin position="201"/>
            <end position="324"/>
        </location>
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        <location>
            <begin position="330"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="32">
        <location>
            <begin position="432"/>
            <end position="525"/>
        </location>
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        <location>
            <begin position="526"/>
            <end position="612"/>
        </location>
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        <location>
            <begin position="785"/>
            <end position="874"/>
        </location>
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        <location>
            <begin position="959"/>
            <end position="1047"/>
        </location>
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        <location>
            <begin position="1049"/>
            <end position="1139"/>
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        <location>
            <begin position="1140"/>
            <end position="1235"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="32">
        <location>
            <begin position="1390"/>
            <end position="1478"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="32">
        <location>
            <begin position="1651"/>
            <end position="1746"/>
        </location>
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        <location>
            <begin position="1751"/>
            <end position="1840"/>
        </location>
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        <location>
            <begin position="1845"/>
            <end position="1935"/>
        </location>
    </feature>
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        <location>
            <begin position="1940"/>
            <end position="2029"/>
        </location>
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            <begin position="2034"/>
            <end position="2133"/>
        </location>
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        <location>
            <begin position="2138"/>
            <end position="2230"/>
        </location>
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        <location>
            <begin position="2232"/>
            <end position="2322"/>
        </location>
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        <location>
            <begin position="2327"/>
            <end position="2420"/>
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        <location>
            <begin position="2424"/>
            <end position="2510"/>
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        <location>
            <begin position="2514"/>
            <end position="2618"/>
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        <location>
            <begin position="2623"/>
            <end position="2721"/>
        </location>
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        <location>
            <begin position="2726"/>
            <end position="2827"/>
        </location>
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        <location>
            <begin position="3076"/>
            <end position="3332"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="31">
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            <begin position="3252"/>
            <end position="3323"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004247"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="3372" mass="378834" checksum="90C09769C8E07E7A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EY29</accession>
    <name>A0A4W2EY29_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </source>
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        </source>
    </evidence>
    <sequence length="343" mass="39401" checksum="05DA5FC7EF10B141" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQADFRKARGTRDQIANIHWIMEKAREFQKSIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQGCILSPCLFNFYAEYIMRNAGLEETQAGIKIAGRHINHLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVVTNLDSILKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAERQRIDAFELWCWRRLLGVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYN7</accession>
    <name>A0A4W2EYN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mucin-1</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EYN7"/>
    <dbReference type="SMR" id="A0A4W2EYN7"/>
    <dbReference type="STRING" id="30522.A0A4W2EYN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039826.1">
        <property type="protein sequence ID" value="ENSBIXP00000042179.1"/>
        <property type="gene ID" value="ENSBIXG00000026287.1"/>
    </dbReference>
    <dbReference type="OMA" id="TTPVHNG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10006:SF19">
        <property type="entry name" value="MUCIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10006">
        <property type="entry name" value="MUCIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
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    <sequence length="663" mass="65645" checksum="4021CE378241158F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    </dbReference>
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        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF19">
        <property type="entry name" value="OLFACTORY RECEPTOR 6C68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="311" mass="35717" checksum="0A0FB5CEE0C2D6AF" modified="2019-09-18" version="1">MRNHTTVTLFILLGLTDDPQLQILLFIFLFITYILSITGNLTIITLTLMDPHLKTPMYFFLQNFSFLEILFTTTCIPRFLYNLSTGDRVITYKACFIQLFLTDLFGVTEFFLLATMSYDRYVAICKPLHYMTIMSNKLCKTMVICCWMAALMTILPPLSLDFHLEFCDSNIIDHFACDVSPLLKISCSDTWLIEQMVIACSVLILIITLLCVVLSYIYIIRTILKFPSLQQRKKAFFTCSSHMIVLSISYGSCIFIYIKPSAKEEVNINKGVSLLISSISPMLNLFIYTLRNKQVKQAFDGSLKKIAFLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3T4</accession>
    <name>A0A4W2F3T4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">RAB3D</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB3D may be involved in the insulin-induced exocytosis of GLUT4-containing vesicles in adipocytes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Cell membrane</location>
            <topology evidence="2 21">Lipid-anchor</topology>
            <orientation evidence="2 21">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027403487.1">
        <property type="nucleotide sequence ID" value="XM_027547686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3T4"/>
    <dbReference type="SMR" id="A0A4W2F3T4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043268.1">
        <property type="protein sequence ID" value="ENSBIXP00000044092.1"/>
        <property type="gene ID" value="ENSBIXG00000028314.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042443.1">
        <property type="protein sequence ID" value="ENSBIXP00005044781.1"/>
        <property type="gene ID" value="ENSBIXG00005002375.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895888"/>
    <dbReference type="CTD" id="9545"/>
    <dbReference type="GeneTree" id="ENSGT00940000157552"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042588">
        <property type="term" value="C:zymogen granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030742">
        <property type="term" value="F:GTP-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031489">
        <property type="term" value="F:myosin V binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045453">
        <property type="term" value="P:bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903307">
        <property type="term" value="P:positive regulation of regulated secretory pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01865">
        <property type="entry name" value="Rab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000448">
        <property type="entry name" value="RAB3D, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037872">
        <property type="entry name" value="Rab3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="15 21" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17 21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 21" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18 21" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13 21" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="189"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU367096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="219" mass="24387" checksum="88593BFA73C035F1" modified="2019-09-18" version="1">MASAGDPPTGQRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDVANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPTEEGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSPGSNGKGPALGDTPAPQPSSCPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FIC9</accession>
    <name>A0A4W2FIC9_BOBOX</name>
    <protein>
        <submittedName>
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            <taxon>Bos</taxon>
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    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24192:SF2">
        <property type="entry name" value="ANKRD40 C-TERMINAL-LIKE PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 40"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="113" mass="13186" checksum="7965163157A6CE6A" modified="2019-09-18" version="1">MAEPQLDVKKPSVRIQSHKENDFIEVELDRQELSYQNLLQVSCYELGINPEQVEKIRKLPNTLLRKDKDILRLQDFQEVELILMKNGSSELAEYTPSLLEKPCYNSNAAKMTY</sequence>
</entry>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the FAM168 family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00005007154.1"/>
        <property type="gene ID" value="ENSBIXG00005012453.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000005140"/>
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    </dbReference>
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        <property type="entry name" value="FAM168A/MANI"/>
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        <property type="entry name" value="TCRP1"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="195" mass="20284" checksum="DDF03CBE6E16C7DB" modified="2019-09-18" version="1">MNPVYSPGSSGVPYANAKGIGYPAGFPMGYAAAAPAYSPNMYPGANPTFQAGYTSGTPYKVSCSPTSGAVPPYSSSPNPYQTAVYPVRSAYPQQSPYAQQGTYYTQPLYAAPPHVIHHTTVVQPNGMPATVYPAPLPPPRGNGVTMGMVAGTTMAMSAGTLLTAHSPTPVAPHPVTVPTYRAPGTPTYSYVPPQW</sequence>
</entry>
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    <accession>A0A4W2FSY7</accession>
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        <submittedName>
            <fullName evidence="3">MAGE family member D2</fullName>
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        <name evidence="3" type="primary">MAGED2</name>
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    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_027533771.1"/>
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        <property type="nucleotide sequence ID" value="XM_027533772.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027533773.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027389575.1">
        <property type="nucleotide sequence ID" value="XM_027533774.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000005962.1">
        <property type="protein sequence ID" value="ENSBIXP00000005341.1"/>
        <property type="gene ID" value="ENSBIXG00000011561.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005016646.1">
        <property type="protein sequence ID" value="ENSBIXP00005008899.1"/>
        <property type="gene ID" value="ENSBIXG00005014025.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Melanoma-associated antigen D1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="melanoma-associated antigen D1"/>
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        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN D2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <end position="254"/>
        </location>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008899.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="588" mass="63207" checksum="69C7EF333176F78C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FUC0</accession>
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        <submittedName>
            <fullName evidence="9">Golgi transport 1A</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="primary">GOLT1A</name>
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    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="7">Belongs to the GOT1 family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF245">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN GOT1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="132" mass="14817" checksum="111BB63777A19B0F" modified="2019-09-18" version="1">MISITEWQKIGVGTTGFGIFFILFGMLLYFDSVLLAFGNLLFLTGLSLIIGLRRTFSFFFQRHKFKGTSFFLGGVVIVLLRWPLLGMCLETYGFFSLFRGFFPVAFGFLGSASNIPFLSALFQRLQGTSSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G0L1</accession>
    <name>A0A4W2G0L1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G0L1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034874.1">
        <property type="protein sequence ID" value="ENSBIXP00000020668.1"/>
        <property type="gene ID" value="ENSBIXG00000023945.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020967.1">
        <property type="protein sequence ID" value="ENSBIXP00005011860.1"/>
        <property type="gene ID" value="ENSBIXG00005016221.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="123" mass="13331" checksum="65797BC014047D1D" modified="2019-09-18" version="1">MHLCRATLTRYHCSNYCCCRTGSFLVACWQVGHCSSMPEARLDTGTGGDSGRFILGGEVEAQASGLCWGWWWVGSSGKSLEGSWPGGNHRERCSCSWESEAECCSWVLLCRVGSGAGRLGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GC70</accession>
    <name>A0A4W2GC70_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CDC42 small effector protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CDC42SE2</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with CDC42 (in GTP-bound form). Interacts weakly with RAC1 and not at all with RHOA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Phagocytic cup</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDC42SE/SPEC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402848.1">
        <property type="nucleotide sequence ID" value="XM_027547047.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402849.1">
        <property type="nucleotide sequence ID" value="XM_027547048.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402850.1">
        <property type="nucleotide sequence ID" value="XM_027547049.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402851.1">
        <property type="nucleotide sequence ID" value="XM_027547050.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402852.1">
        <property type="nucleotide sequence ID" value="XM_027547051.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402854.1">
        <property type="nucleotide sequence ID" value="XM_027547053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GC70"/>
    <dbReference type="STRING" id="30522.A0A4W2GC70"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049396.1">
        <property type="protein sequence ID" value="ENSBIXP00000020398.1"/>
        <property type="gene ID" value="ENSBIXG00000023709.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026372.1">
        <property type="protein sequence ID" value="ENSBIXP00005015494.1"/>
        <property type="gene ID" value="ENSBIXG00005019311.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895634"/>
    <dbReference type="CTD" id="56990"/>
    <dbReference type="GeneTree" id="ENSGT00940000158245"/>
    <dbReference type="OMA" id="CCIGGQP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001891">
        <property type="term" value="C:phagocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000004">
        <property type="entry name" value="CDC42 small effector protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039056">
        <property type="entry name" value="SPEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502:SF4">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="16">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005015494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9197" checksum="98C5E82176CF8C0A" modified="2019-09-18" version="1">MSEFWLCFNCCIAEQPQPKRRRRIDRSMIGEPTNFVHTAHVGSGDLFSGMNSVSSIQNQMQSKGGYGGGMAANVQMQLVDTKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H5M6</accession>
    <name>A0A4W2H5M6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lariat debranching enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DBR1</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lariat debranching enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401106.1">
        <property type="nucleotide sequence ID" value="XM_027545305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H5M6"/>
    <dbReference type="SMR" id="A0A4W2H5M6"/>
    <dbReference type="STRING" id="30522.A0A4W2H5M6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040378.1">
        <property type="protein sequence ID" value="ENSBIXP00000024303.1"/>
        <property type="gene ID" value="ENSBIXG00000007546.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040509.1">
        <property type="protein sequence ID" value="ENSBIXP00005024992.1"/>
        <property type="gene ID" value="ENSBIXG00005027529.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894628"/>
    <dbReference type="CTD" id="51163"/>
    <dbReference type="GeneTree" id="ENSGT00510000047481"/>
    <dbReference type="OMA" id="GIDDPLC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008419">
        <property type="term" value="F:RNA lariat debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000292">
        <property type="term" value="P:RNA fragment catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00844">
        <property type="entry name" value="MPP_Dbr1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000035">
        <property type="entry name" value="Lariat debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007708">
        <property type="entry name" value="DBR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041816">
        <property type="entry name" value="Dbr1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849:SF0">
        <property type="entry name" value="LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849">
        <property type="entry name" value="RNA LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05011">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01124">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lariat debranching enzyme C-terminal" evidence="15">
        <location>
            <begin position="235"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="394"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="476"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="397"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="544" mass="61420" checksum="5E6E72E41FD8E41F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HHM4</accession>
    <name>A0A4W2HHM4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Malignant T-cell-amplified sequence</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC113903400</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MCTS1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027559479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HHM4"/>
    <dbReference type="SMR" id="A0A4W2HHM4"/>
    <dbReference type="STRING" id="30522.A0A4W2HHM4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025597.1">
        <property type="protein sequence ID" value="ENSBIXP00000014621.1"/>
        <property type="gene ID" value="ENSBIXG00000019254.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003568.1">
        <property type="protein sequence ID" value="ENSBIXP00005031816.1"/>
        <property type="gene ID" value="ENSBIXG00005012253.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00550000074964"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malignant T-cell-amplified sequence 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="6">
        <location>
            <begin position="93"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="181" mass="20590" checksum="A9EE2597F82FBCA5" modified="2019-09-18" version="1">MFKKFDEKENVSNCIQLKTSVIKGIKNQLMEQFPGIEPWLNQIMPKKDPVKIVRCHEHIEILTVNGELLFFRQREGPFYPTLRLLHKYPFILPRQQVDKGAIKFVLSGANIMCPGLTSPGARLFPAAVDTVVAIMAEGKQHALCVGVMKMSADEIEKVNKGIGIENIHYLNDGLWHMKTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HJ10</accession>
    <name>A0A4W2HJ10_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone-lysine N-methyltransferase EZH2</fullName>
            <ecNumber evidence="2">2.1.1.356</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EZH2</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.356" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027394529.1">
        <property type="nucleotide sequence ID" value="XM_027538728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HJ10"/>
    <dbReference type="SMR" id="A0A4W2HJ10"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034667.1">
        <property type="protein sequence ID" value="ENSBIXP00000020534.1"/>
        <property type="gene ID" value="ENSBIXG00000023837.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005233.1">
        <property type="protein sequence ID" value="ENSBIXP00005031266.1"/>
        <property type="gene ID" value="ENSBIXG00005011741.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891050"/>
    <dbReference type="CTD" id="2146"/>
    <dbReference type="GeneTree" id="ENSGT00940000155013"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="757" mass="86451" checksum="171CDE9769E4C92D" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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        <name evidence="16" type="primary">PRKAG2</name>
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            <taxon>Pecora</taxon>
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        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000020988.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005033965.1"/>
        <property type="gene ID" value="ENSBIXG00005006133.1"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183019"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:phosphorylase kinase regulator activity"/>
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        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
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    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13" id="KW-0129">CBS domain</keyword>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
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            <end position="331"/>
        </location>
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        <location>
            <begin position="353"/>
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    <feature type="domain" description="CBS" evidence="15">
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        <location>
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        <location>
            <begin position="1"/>
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            <begin position="65"/>
            <end position="77"/>
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            <begin position="132"/>
            <end position="144"/>
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            <begin position="156"/>
            <end position="165"/>
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        <location>
            <begin position="178"/>
            <end position="187"/>
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        <location>
            <begin position="190"/>
            <end position="202"/>
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        <location>
            <begin position="203"/>
            <end position="232"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51371"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="565" mass="62417" checksum="5CAE84FC772A22BC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HW31</accession>
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        <recommendedName>
            <fullName evidence="8">Malic enzyme</fullName>
        </recommendedName>
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        <name evidence="11" type="primary">ME3</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <cofactor evidence="7">
            <name>Mg(2+)</name>
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        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
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        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the malic enzymes family.</text>
    </comment>
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        <property type="component" value="Chromosome 29"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051287">
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        <property type="term" value="P:pyruvate biosynthetic process"/>
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        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK13529.1"/>
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    </dbReference>
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        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF20">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="114"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="305"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
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    <sequence length="604" mass="67210" checksum="98ADADB56133C376" modified="2019-09-18" version="1"> </sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="318" mass="36536" checksum="83AF4D7C78342628" modified="2019-09-18" version="1">MRLLAGWLCLSLASVWLARRMWTLRSPLTRSLYVNMTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQKTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVENVGGKWDKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV</sequence>
</entry>
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    <accession>A0A4W2I8U8</accession>
    <name>A0A4W2I8U8_BOBOX</name>
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    <organism evidence="10 12">
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        <lineage>
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    <reference evidence="10 11 12" key="1">
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902622">
        <property type="term" value="P:regulation of neutrophil migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034143">
        <property type="term" value="P:regulation of toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002238">
        <property type="term" value="P:response to molecule of fungal origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032494">
        <property type="term" value="P:response to peptidoglycan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043588">
        <property type="term" value="P:skin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008063">
        <property type="term" value="P:Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034146">
        <property type="term" value="P:toll-like receptor 5 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034158">
        <property type="term" value="P:toll-like receptor 8 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038124">
        <property type="term" value="P:toll-like receptor TLR6:TLR2 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060337">
        <property type="term" value="P:type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08312">
        <property type="entry name" value="Death_MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000029">
        <property type="entry name" value="Myeloid differentiation primary response protein MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000005">
        <property type="entry name" value="Myeloid differentiation primary response protein MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034249">
        <property type="entry name" value="MyD88_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017281">
        <property type="entry name" value="Myelin_different_resp_MyD88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15079">
        <property type="entry name" value="MYD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15079:SF3">
        <property type="entry name" value="MYELOID DIFFERENTIATION PRIMARY RESPONSE PROTEIN MYD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="8">
        <location>
            <begin position="120"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="9">
        <location>
            <begin position="247"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="384" mass="42386" checksum="1131E6DB49CE8F1E" modified="2019-09-18" version="1">MGGAQNTSKGGFRIPTSPAPPPRDLEGSDGGPPASAHLLGLQIVGQGGASRKERPAGRDQVGGAAEGEGGSTGPGRDSTARRLPRQPAMAEGVPRAGSALPAASLSSLPLAALNVRVRRRLSLFLNVRAPVAADWTVLAEAMDFEYLEIQQLEKYADPTSRLLDDWQRRPGASVGRLLELLAKLGRDDVLMELGPSIEEDCQKYILKQQQEASEKPLQVDSIDSSITRINDMAGITIRDDPLGQKPECFDAFICYCPSDIEFVHEMIRQLEQTNYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDEYLQSKECDFQTKFALSLSPGAHQKRLIPIKYKPMKKEFPSILRFITVCDYTNPCTQNWFWTRLAKALSMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ING4</accession>
    <name>A0A4W2ING4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IQ motif containing M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">IQCM</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ING4"/>
    <dbReference type="STRING" id="30522.A0A4W2ING4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004685.1">
        <property type="protein sequence ID" value="ENSBIXP00000005961.1"/>
        <property type="gene ID" value="ENSBIXG00000012113.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054245.1">
        <property type="protein sequence ID" value="ENSBIXP00005045749.1"/>
        <property type="gene ID" value="ENSBIXG00005029236.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010038"/>
    <dbReference type="OMA" id="KMRQHYK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35978">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35978:SF1">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="501" mass="58786" checksum="AE2284DC6AEAE3C0" modified="2019-09-18" version="1">MAAEAASDKAGSSILEIPKQDFFQEAKTLIAQHYKKINENKVPGTSINVFRNKHQKPKSTKFIPLEIKKKETLDAVQELQAAYKRICFPKDHSKSGPSEEPPQRTAFSEPHIFNIKKKHKEPIDLIVKEQVKLDKIVTNIESVSKKMEKEKQLHHGKTRNRKLDSFSAFPVFNLGLHFRPTTSSSMGQLKEHDRTLYDWRGSVSKRFSRLTHDSSWPSFSGSSLAFQDYSSKNFIKKEQQSVKPESKPQPSPKSILSKSDKIDNKVKRIGPHIEIFQVFRGRKKFAITKNIIKMVTIMQALVRGWLERKRFQRIMTKALYHGPDLRTVINMYCRQIHRVKYRLGLWRTRQIINLTELEEWMDRKKFYETMFAKREDWQGLEKSELLKYFNDCGHFPTHQQIDEVWDLVHREDREKYSDIIKKHNAIEMLFTLYPPQGAHVSTNLRLRSTWLRPIVNGEEGYKYIVSGHPVLKRANIRIVGKLVAASIRERKMRQRYSSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BKI1</accession>
    <name>A0A4W2BKI1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Preferentially expressed antigen in melanoma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRAME family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BKI1"/>
    <dbReference type="STRING" id="30522.A0A4W2BKI1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053352.1">
        <property type="protein sequence ID" value="ENSBIXP00000000328.1"/>
        <property type="gene ID" value="ENSBIXG00000029951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000354">
        <property type="entry name" value="Melanoma antigen preferentially expressed in tumors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050694">
        <property type="entry name" value="LRRC14/PRAME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026271">
        <property type="entry name" value="PRAME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224:SF24">
        <property type="entry name" value="MELANOMA ANTIGEN PREFERENTIALLY EXPRESSED IN TUMORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224">
        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038286">
        <property type="entry name" value="PRAME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="60943" checksum="5BB4C39D1E29150C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMN3</accession>
    <name>A0A4W2BMN3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMN3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014617.1">
        <property type="protein sequence ID" value="ENSBIXP00000001173.1"/>
        <property type="gene ID" value="ENSBIXG00000028861.1"/>
    </dbReference>
    <dbReference type="OMA" id="CELWKIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="412" mass="47463" checksum="EDACC9A51D1541DA" modified="2019-09-18" version="1">MLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANICWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPEHLICLLRNWYAGQEATVRTGHGTTDWFQIGKGVHQGCILSPCLFNLYAEYIMRNAGLEETQAGIRIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLVSKITADGDCSYEIKRRLLLGRKLMTNLDSIFKSRDITLPTKVHLVKAMVFPVVMYGCESWPVKKAECRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYSGHLMRRVDSLEKTLMLGGIGVKRRKGRQRMRWLDGITDLMDVSLSELRELVMDREAWRAAIHGVAKSQTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNV4</accession>
    <name>A0A4W2BNV4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Small nuclear ribonucleoprotein Sm D1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">snRNP core protein D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNRPD1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10 12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Most spliceosomal snRNPs contain a common set of Sm proteins, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Component of the minor spliceosome, which splices U12-type introns. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG; catalyzes core snRNPs assembly. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts (via C-terminus) with SMN1 (via Tudor domain); the interaction is direct. Interacts with GEMIN2; the interaction is direct. Interacts with SNRPD2; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
        <text evidence="12">SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNV4"/>
    <dbReference type="STRING" id="30522.A0A4W2BNV4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000329.1">
        <property type="protein sequence ID" value="ENSBIXP00000000709.1"/>
        <property type="gene ID" value="ENSBIXG00000029466.1"/>
    </dbReference>
    <dbReference type="OMA" id="CVNVSMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01724">
        <property type="entry name" value="Sm_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000016">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034102">
        <property type="entry name" value="Sm_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="119" mass="13271" checksum="A877670F33050C5F" modified="2019-09-18" version="1">MKLARFLMKLSHETVTIELKNGTQVHGTITCVNVSMNTHLKAVKMTLKNREPVQLETLSIRGNNIWYFILPDSLPLDTLLVGVEPKVKSKKREAVAGRGRGRGRGRGRGRGRGRGGPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQG5</accession>
    <name>A0A4W2BQG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase family 2 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQG5"/>
    <dbReference type="STRING" id="30522.A0A4W2BQG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014206.1">
        <property type="protein sequence ID" value="ENSBIXP00000001362.1"/>
        <property type="gene ID" value="ENSBIXG00000028655.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019391">
        <property type="term" value="P:glucuronoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066:SF67">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="2">
        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="71" mass="8242" checksum="E4661800F1D4790D" modified="2019-09-18" version="1">PDCKDPPLMFSEEYQKGLPQQYHVVLDQKRKEYVVGELIWNFADFMTNQCKWHLDAWDVSLLIVSGGLLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTG0</accession>
    <name>A0A4W2BTG0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTG0"/>
    <dbReference type="STRING" id="30522.A0A4W2BTG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009963.1">
        <property type="protein sequence ID" value="ENSBIXP00000003386.1"/>
        <property type="gene ID" value="ENSBIXG00000009796.1"/>
    </dbReference>
    <dbReference type="OMA" id="CEYNDTW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF159">
        <property type="entry name" value="SERINE PROTEASE 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021272416" description="Peptidase S1 domain-containing protein" evidence="10">
        <location>
            <begin position="25"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="80"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="322" mass="34835" checksum="5EAA97A28CB938E3" modified="2019-09-18" version="1">MASLGGPHTLLVWLLLLQPWLTEASEDGSATSLPPPAPSGGDAKNRTVAQPPLRGASEVPKLVEFSLGDSQPCGQVFLKILGGHDSEEGKWPWQVSLRVRNRHVCGASLVTERWVLTAGHCILSRYQYSVMMGDRKLQGTLSGLVVPVSRVVVHPQFSTSGTVKNDLALLRLRYPVNFTGVIQPICIPEKTFRVQAGTRCWVTGWGRKQEFEGPLVSEMALRTDKDLVQEGVICGYNSIGKDSCQGDSGGPLVCQYSTTWVQVGIVSWGVGCGRNNTPGVYTETALYAKWLVAVVNKAASLYPVVLLLLLCVVLPLGLLVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUY8</accession>
    <name>A0A4W2BUY8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribosomal protein S6 kinase beta-2</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">70 kDa ribosomal protein S6 kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">p70 ribosomal S6 kinase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">RPS6KB2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027388676.1">
        <property type="nucleotide sequence ID" value="XM_027532875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUY8"/>
    <dbReference type="SMR" id="A0A4W2BUY8"/>
    <dbReference type="STRING" id="30522.A0A4W2BUY8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008772.1">
        <property type="protein sequence ID" value="ENSBIXP00000003989.1"/>
        <property type="gene ID" value="ENSBIXG00000010354.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886548"/>
    <dbReference type="CTD" id="6199"/>
    <dbReference type="OMA" id="AGTHCIA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05584">
        <property type="entry name" value="STKc_p70S6K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000587">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000092">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="67"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="19">
        <location>
            <begin position="329"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="408"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="485" mass="53778" checksum="E7E0AB11167C452C" modified="2019-09-18" version="1">MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTESSVNPGPERIGPHCFELLRVLGKGNYGKVFQVRKVQGSNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLSEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAENRKKTMDKIIRGKLELPPYLTPDARDLVKKFLKRNPNQRIGGGPGDAADVQRHPFFRHVNWDDLLARRVDPPFRPSLQSEEDVSQFDSHFTKQTPVDSPDDTTLSESANQAFLGFTYVAPSVLDSIKEAFSFQPKLRSPRRLNSSPRTPVSPLKFSPFEGFRPSPGPQELVEPPLPPLLPPPPPPPPSSTAPLPIRPPSGTKKSKRGRGRPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BX47</accession>
    <name>A0A4W2BX47_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SKP1 component POZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BX47"/>
    <dbReference type="STRING" id="30522.A0A4W2BX47"/>
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        <property type="protein sequence ID" value="ENSBIXP00000004886.1"/>
        <property type="gene ID" value="ENSBIXG00000011183.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010883.1">
        <property type="protein sequence ID" value="ENSBIXP00005029414.1"/>
        <property type="gene ID" value="ENSBIXG00005009321.1"/>
    </dbReference>
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    <dbReference type="OMA" id="QWFGRRY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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        <property type="entry name" value="SKP1"/>
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        <property type="entry name" value="SKP1-like"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Skp1"/>
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    </dbReference>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8857" checksum="7AE88A7944B0D3A1" modified="2019-09-18" version="1">MPSIKLQSSDGEIFKVDAEIAKQSVTIKTILEDLGMDDEGDDDPVPLPNVNAAILKKVIQWSLKAQLLLSQWFGRRYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZE9</accession>
    <name>A0A4W2BZE9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZE9"/>
    <dbReference type="STRING" id="30522.A0A4W2BZE9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007534.1">
        <property type="protein sequence ID" value="ENSBIXP00000004577.1"/>
        <property type="gene ID" value="ENSBIXG00000010904.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="104" mass="12123" checksum="0B19A9256A1F5482" modified="2019-09-18" version="1">RNVRKESLFCYYLLNLTLQRQKPSLKRLLQRREKRDPKTKGEKLTVVRKGITLKKMEMPSQTTQRKTKVLGIPNSVFNSVSYILRSGILDCMVIAFPILEQIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C017</accession>
    <name>A0A4W2C017_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CUE domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CUEDC2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CUEDC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027385283.1">
        <property type="nucleotide sequence ID" value="XM_027529482.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385284.1">
        <property type="nucleotide sequence ID" value="XM_027529483.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385285.1">
        <property type="nucleotide sequence ID" value="XM_027529484.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385286.1">
        <property type="nucleotide sequence ID" value="XM_027529485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C017"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004545.1">
        <property type="protein sequence ID" value="ENSBIXP00000006035.1"/>
        <property type="gene ID" value="ENSBIXG00000012181.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001609.1">
        <property type="protein sequence ID" value="ENSBIXP00005007808.1"/>
        <property type="gene ID" value="ENSBIXG00005012994.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884673"/>
    <dbReference type="CTD" id="79004"/>
    <dbReference type="GeneTree" id="ENSGT00390000014513"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900016">
        <property type="term" value="P:negative regulation of cytokine production involved in inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010936">
        <property type="term" value="P:negative regulation of macrophage cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14367">
        <property type="entry name" value="CUE_CUED2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039805">
        <property type="entry name" value="CUE_CUED2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493">
        <property type="entry name" value="CUE DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493:SF0">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="8">
        <location>
            <begin position="141"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31532" checksum="FAC6CAB96CA75313" modified="2019-09-18" version="1">MELERIVSAALLAFVQTHLPEADLSGLDDVIFSYVLGVLGDLGPSGPSEENFDMEAFTEMMEAYVPGFAHIPRGTIGDMIQKLSGQLSGARNKENVQPQSSEVQGQVSISPEPLQRPEKLKEETMSSAGDTQDEAAGPEEELLPGVDVLLEVFPTCSVEQAQWVLAKARGDLEEAVQMLVEGKQGPPAWDGPNQDLPRRLRGPQKDELKSFILQKYMMVDSAEDQKIHRPMAPKEAPKKLIRYIDNQIVSTKGERFKDVRNPEAEEMKATYINLKPARKYRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C156</accession>
    <name>A0A4W2C156_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fibrinogen gamma chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FGG</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027369305.1">
        <property type="nucleotide sequence ID" value="XM_027513504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C156"/>
    <dbReference type="SMR" id="A0A4W2C156"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003836.1">
        <property type="protein sequence ID" value="ENSBIXP00000006401.1"/>
        <property type="gene ID" value="ENSBIXG00000012517.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040518.1">
        <property type="protein sequence ID" value="ENSBIXP00005043796.1"/>
        <property type="gene ID" value="ENSBIXG00005027566.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875087"/>
    <dbReference type="CTD" id="2266"/>
    <dbReference type="GeneTree" id="ENSGT00940000158467"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072377">
        <property type="term" value="P:blood coagulation, common pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.50:FF:000003">
        <property type="entry name" value="Fibrinogen gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000002">
        <property type="entry name" value="Fibrinogen gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.530.10:FF:000002">
        <property type="entry name" value="Fibrinogen gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012290">
        <property type="entry name" value="Fibrinogen_a/b/g_coil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF9">
        <property type="entry name" value="FIBRINOGEN GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08702">
        <property type="entry name" value="Fib_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01212">
        <property type="entry name" value="Fib_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58010">
        <property type="entry name" value="Fibrinogen coiled-coil and central regions"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610329" evidence="12">
        <location>
            <begin position="25"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="13">
        <location>
            <begin position="168"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="435" mass="49167" checksum="ECC9E8999BA313BE" modified="2019-09-18" version="1">MSWSSHPPSVIFYILSLLSSACLAYVATRDNCCILDERFGSYCPTTCGIADFLNNYQTSVDKDLRTLEGILYQVENKTSEARELVKAIQISYNPDQPSKPNNIESATKNSKSMMEEIMKYETLISTHESTIRFLQEIYNSNSQKIVNLRDKVVQLEANCQEPCQDTVKIHDVTGRDCQDVANKGAKESGLYFIRPLKAKQQFLVYCEIDGSGNGWTVFQKRLDGSLDFKKNWIQYKEGFGHLSPTGNTEFWLGNEKIHLISTQSTIPYVLRIQLEDWNGRTSTADYASFKVTGENDKYRLTYAYFIGGDAGDAFDGYDFGDDSSDKFFTSHNGMQFSTWDSDNDKYDGNCAEQDGSGWWMNKCHAGHLNGVYYQGGTYSKTSTPNGYDNGIIWATWKSRWYSMKKTTMKIIPLNRLAIGEGQQHQLGGAKQAGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C195</accession>
    <name>A0A4W2C195_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CSGALNACT2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-(beta-D-GalNAc-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23464"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:12575"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:132105"/>
            <dbReference type="EC" id="2.4.1.174"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus</location>
            <location evidence="1 13">Golgi stack membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C195"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005647.1">
        <property type="protein sequence ID" value="ENSBIXP00000005497.1"/>
        <property type="gene ID" value="ENSBIXG00000000955.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047237">
        <property type="term" value="F:glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000059">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF20">
        <property type="entry name" value="CHONDROITIN SULFATE N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="98"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="575" mass="66281" checksum="3E3BB548A57AB361" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C227</accession>
    <name>A0A4W2C227_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 200</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF200</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C227"/>
    <dbReference type="STRING" id="30522.A0A4W2C227"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053372.1">
        <property type="protein sequence ID" value="ENSBIXP00000000320.1"/>
        <property type="gene ID" value="ENSBIXG00000029968.1"/>
    </dbReference>
    <dbReference type="OMA" id="HEVIHVK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001771">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001830">
        <property type="entry name" value="zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002005">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF404">
        <property type="entry name" value="ZINC FINGER PROTEIN 432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="279"/>
            <end position="306"/>
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        <location>
            <begin position="363"/>
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        </location>
    </feature>
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        <location>
            <begin position="160"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="394" mass="45391" checksum="1042EB8E900E2EE3" modified="2019-09-18" version="1">TMAAKVVPMPPKPKRSFILRVPPNSKLGQDLLRDATSGPKTIHQLVLEHFLTFLPKPSLVQPSQKIKETLVIVKDVSSNLQNRGQPRPLVKLLPREEIQQKQETESLCLKSESEKLVVFEDLNVFHSQEECVSLDPAQQPTSEMEEDSIGEMMLLVSGSNPEGEELQTEPVEDEDHREESSDCDEMDCSMVPEQSPDCQKEERLNTSIPKERKMRNLLVTIENDTPLEELSKYVDINVIALTRNRRTRRWYTCPLCGKQFNESSYLISHQRTHTGEKPYDCSHCGKSFNHKTNLNKHERIHTGEKPYSCSQCGKNFRQNSHRSRHEGIHIREKILKCPECGKTFPENKEFVIHLQSHKAKRPYGCKKCGRRFGRLSNCTRHEKTHSACKTRKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3I3</accession>
    <name>A0A4W2C3I3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger and SCAN domain containing 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113881606</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3I3"/>
    <dbReference type="STRING" id="30522.A0A4W2C3I3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002049.1">
        <property type="protein sequence ID" value="ENSBIXP00000007335.1"/>
        <property type="gene ID" value="ENSBIXG00000013308.1"/>
    </dbReference>
    <dbReference type="OMA" id="KQRGHPT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001087">
        <property type="entry name" value="Zinc finger and SCAN domain-containing protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000380">
        <property type="entry name" value="zinc finger protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001530">
        <property type="entry name" value="Zinc finger protein 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="14">
        <location>
            <begin position="39"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="50288" checksum="F8A6FFAF49B3077E" modified="2019-09-18" version="1">MASAGEQDGLKIVKVEEDPIWDQEPYLRENNFSGQEASRQLFRHFCYQETPGPREALSRLRELCHQWLRPDTHTKEQILELLVLEQFLTILPEELQAWVCEHHPESGEEAVAAVEDLERELSEPENQAPDCEHGLAEMLSGDAVHLKAKEESTAFQLQSMVMQLKCESFGVHKFGEQDGETVLKNQDLTSKQEVLKEMGHFENSRLQKDIPLDSKYREMCKHESREEKQSGQPTGERCHKCNECGKSFTQSSVLIQHQRIHTGEKPYECEECGKTFSQRSGLIEHQRSHTGEKPYQCKDCGKAFSASNSLIRHRRIHTGEKPYECEECGKTFRLSSYLVQHQRIHTGEKRYHCNECGKAFSQNAGLFQHLRIHTGEKPYQCGQCSKSFSRRTLLIKHQRSHTGERPYECDECGKAFSHHCNLIRHFRIHTVVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C500</accession>
    <name>A0A4W2C500_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C500"/>
    <dbReference type="STRING" id="30522.A0A4W2C500"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002930.1">
        <property type="protein sequence ID" value="ENSBIXP00000006872.1"/>
        <property type="gene ID" value="ENSBIXG00000012939.1"/>
    </dbReference>
    <dbReference type="OMA" id="GVYDCPF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000038">
        <property type="entry name" value="achaete-scute homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF108">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="189" mass="20085" checksum="EFDFA9B01BF7345D" modified="2019-09-18" version="1">MARLRGTSEPPPRAEISADQSVLPEPRPCLPLGIGPPPRPAPPAGAEPLGAVPFLLYPGAAGPPYCDAYAAAFPGAPFPGALGACDYPFEPAFIQKRNERERQRVRCVNEGYARLRGHLPGALAEKRLSKVETLRAAIRYIKHLQELLSAAPDGAAPREPRAAPSLVPDSAEPSCGSSSPFFESEECSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C518</accession>
    <name>A0A4W2C518_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C518"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003324.1">
        <property type="protein sequence ID" value="ENSBIXP00000006652.1"/>
        <property type="gene ID" value="ENSBIXG00000012759.1"/>
    </dbReference>
    <dbReference type="OMA" id="EICEIYE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000003">
        <property type="entry name" value="IgG receptor FcRn large subunit p51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF154">
        <property type="entry name" value="MHC CLASS I POLYPEPTIDE-RELATED SEQUENCE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="200"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="367" mass="41111" checksum="3D1FDE44F301D56F" modified="2019-09-18" version="1">SGLSLSASSVVPPSPGSHSLLYNMTVLSWDSFAQSSFFAEGYLDGQAFLHYDHKKGRAEPRGRWAEKLAAETWETESTVLNATLKELRKLLAEILPLQEEKGGLHSLQETVGCNISEDSHPRGFRLLYFNEKLLLSCYPEPLGCALLQPSAQTLAMEMKPWDTDGFLSKHYQAHVQGELCRRLQAYLESWPGFMERTEPPAVNVTHSQDSESMVHLTGKAFGFFPRTISVVWFRDKEPMSRDTQESGGVLSDGNGTHYTWETVKIPQGEEQRVKCIVEHSGNHSPFSGKTLVHQRSRWIVSIPVAVVFIIGFCVYCYIKKRKTASATGRPEPIRLQDLDQCQTEPTDHNGLTHPEFQSSCQTPAPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C539</accession>
    <name>A0A4W2C539_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CARS1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17774"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C539"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014075.1">
        <property type="protein sequence ID" value="ENSBIXP00000001414.1"/>
        <property type="gene ID" value="ENSBIXG00000028580.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10310">
        <property type="entry name" value="GST_C_CysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000027">
        <property type="entry name" value="Cysteine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1910:FF:000005">
        <property type="entry name" value="Cysteine-tRNA ligase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000228">
        <property type="entry name" value="Cysteinyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="18">
        <location>
            <begin position="126"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="737"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="783"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="737"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="783"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="832" mass="94133" checksum="09F62AB8347D462C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6K5</accession>
    <name>A0A4W2C6K5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Molybdenum cofactor sulfurase</fullName>
            <shortName evidence="6">MCS</shortName>
            <shortName evidence="6">MOS</shortName>
            <shortName evidence="6">MoCo sulfurase</shortName>
            <ecNumber evidence="6">2.8.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Molybdenum cofactor sulfurtransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MOCOS</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:42636"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:82685"/>
            <dbReference type="EC" id="2.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. MOCOS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.9" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6K5"/>
    <dbReference type="STRING" id="30522.A0A4W2C6K5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012977.1">
        <property type="protein sequence ID" value="ENSBIXP00000001906.1"/>
        <property type="gene ID" value="ENSBIXG00000008444.1"/>
    </dbReference>
    <dbReference type="OMA" id="PCTRCQM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008265">
        <property type="term" value="F:molybdenum cofactor sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000096">
        <property type="entry name" value="Molybdenum cofactor sulfurase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03050">
        <property type="entry name" value="MOCOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028886">
        <property type="entry name" value="MoCo_sulfurase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF80">
        <property type="entry name" value="MOLYBDENUM COFACTOR SULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOSC" evidence="8">
        <location>
            <begin position="707"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="496"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="882" mass="97237" checksum="3089E6708D89C88C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7C6</accession>
    <name>A0A4W2C7C6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7C6"/>
    <dbReference type="STRING" id="30522.A0A4W2C7C6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016486.1">
        <property type="protein sequence ID" value="ENSBIXP00000008687.1"/>
        <property type="gene ID" value="ENSBIXG00000014466.1"/>
    </dbReference>
    <dbReference type="OMA" id="KASWGRD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000185">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000771">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000785">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001238">
        <property type="entry name" value="Zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001822">
        <property type="entry name" value="Zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002291">
        <property type="entry name" value="Zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000585">
        <property type="entry name" value="zinc finger protein 784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002208">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF57">
        <property type="entry name" value="ZINC FINGER PROTEIN 154-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="330"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="527"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="182"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="564" mass="61925" checksum="30A986F50BB0C96E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C856</accession>
    <name>A0A4W2C856_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">E74 like ETS transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ELF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027412918.1">
        <property type="nucleotide sequence ID" value="XM_027557117.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027412919.1">
        <property type="nucleotide sequence ID" value="XM_027557118.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027412920.1">
        <property type="nucleotide sequence ID" value="XM_027557119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C856"/>
    <dbReference type="SMR" id="A0A4W2C856"/>
    <dbReference type="STRING" id="30522.A0A4W2C856"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011836.1">
        <property type="protein sequence ID" value="ENSBIXP00000002477.1"/>
        <property type="gene ID" value="ENSBIXG00000008945.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050727.1">
        <property type="protein sequence ID" value="ENSBIXP00005021203.1"/>
        <property type="gene ID" value="ENSBIXG00005006286.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902176"/>
    <dbReference type="CTD" id="1997"/>
    <dbReference type="GeneTree" id="ENSGT00940000157039"/>
    <dbReference type="OMA" id="DEKRMTT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050860">
        <property type="term" value="P:negative regulation of T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <source>
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        </source>
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    <sequence length="614" mass="66572" checksum="DB1CC8B3CA9F6D84" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C870</accession>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bickhart D.M."/>
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                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1272" mass="149786" checksum="F651CEEAE9B41663" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGV9</accession>
    <name>A0A4W2CGV9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGV9"/>
    <dbReference type="STRING" id="30522.A0A4W2CGV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022074.1">
        <property type="protein sequence ID" value="ENSBIXP00000012262.1"/>
        <property type="gene ID" value="ENSBIXG00000017355.1"/>
    </dbReference>
    <dbReference type="OMA" id="IVHTIMA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF87">
        <property type="entry name" value="OLFACTORY RECEPTOR 51F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="59"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="331" mass="36681" checksum="BB3D998EE41093DA" modified="2019-09-18" version="1">MLCLIIQISLSSQPLIMWVLNNTSAQPLTFLLTGIPGLRAAQIWVSIPVCLLYAGALSGNSMILFVVLREQSLHEPMYYFLSMLSATDLSLSLCTLSTTLGVFWFEAQEINLNACVAQMFFLHGFTFMESGVLLAMAFDHFVAICDARRYTTILTNARIAQIGMIVLVVKRLSFCSSMVLSHSYCYHVDLIQLSCTDNRINSILGLFALFSTTGFDCPCILFSYVLIIRSVLSIASIEGRQKAFNTCISHISAVAIFSIPLISLSLVHRYGHSAPPIVHTIMANIFLLIPPVLNPIIYSVKTKQIQKAVIKVLIQKELILLAHALFLTLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGZ4</accession>
    <name>A0A4W2CGZ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">E3 SUMO-protein ligase RanBP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">358 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Nuclear pore complex protein Nup358</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Nucleoporin Nup358</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Ran-binding protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">LOC113901247</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="38 39" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="26">Part of the nuclear pore complex. Forms a complex with NXT1, NXF1 and RANGAP1. Forms a tight complex with RANBP1 and UBE2I. Interacts with SUMO1 but not SUMO2. Interacts with PRKN. Interacts with sumoylated RANGAP1. Interacts with CDCA8. Interacts with PML (isoform PML-4). Interacts with BICD2. Interacts with MCM3AP isoform GANP. Interacts with COX11. Interacts with synaptic plasticity regulator PANTS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the RanBP2 E3 ligase family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CGZ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020393.1">
        <property type="protein sequence ID" value="ENSBIXP00000011175.1"/>
        <property type="gene ID" value="ENSBIXG00000000836.1"/>
    </dbReference>
    <dbReference type="OMA" id="RCIGNHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051168">
        <property type="term" value="P:nuclear export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14684">
        <property type="entry name" value="RanBD1_RanBP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13177">
        <property type="entry name" value="RanBD2_RanBP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14685">
        <property type="entry name" value="RanBD3_RanBP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13178">
        <property type="entry name" value="RanBD4_RanBP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000018">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000020">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000003">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000018">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000114">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022011">
        <property type="entry name" value="IR1-M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12185">
        <property type="entry name" value="IR1-M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    <sequence length="427" mass="48676" checksum="687D492E266D4825" modified="2019-09-18" version="1">KPSDCSLASIKSPWSSSVGSSCMSCRPEWYLGYICQPGTCVPSLSVSTTYHPTSSIFSSMGSNSLFYESVFSSNERETLQLLNNRLAAYLERVRQLEQENVELERQLQEACEFQEPTVGPNYFRYFQIIEELQQKILYSKAENARIVVQIDNTKLAADDFRSKYEMELGLRQLVEADTNGLRQILDELTLCKADLEMQVESLKEELMCLKKNHEEEVGALRCQLGDRLNIEVDAVSPVDLNKVLEEMRCRYEALVETNRRDVEEWFNKQMEELNQQVVTSSEQLQSYQSDIIDLKRTVNTLEIELQTQHSLRDSLENTLTETEARYGSQLAQMQGLVTNVESQLAEIRCDLERQNQEYRVLLDVRARLEAEINTYRGLLDSEDCKLPGNPCSTPSQPPCAPAPSVPRTLCVPRTVCVPCVPCHPSCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHL4</accession>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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            <dbReference type="Ensembl" id="ENSBIXP00000011395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="195" mass="20754" checksum="6FDC4A7CCA5F2B43" modified="2019-09-18" version="1">MTHSCCSPCCQPTCCRTTCCESSCCKPCCPLTCCQTTCCRTTCCRPTCVTTCCQPSCCSKPCCQPTCCESIRCQPSCPPTCYQTSETTCCRTTCRKPTCVTTCCQPTCCVSSSCGQTFSGSSCGQPCCQPACCAPVYCRRICYHPTCCCLPGCQDQSCGSSCCHCCQPCCRPVSCLTTCCRTTSCHPSCCQPSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIB0</accession>
    <name>A0A4W2CIB0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="9">Tripartite motif-containing protein 67</fullName>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING_CS"/>
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    </dbReference>
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        <location>
            <begin position="210"/>
            <end position="253"/>
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        <location>
            <begin position="447"/>
            <end position="505"/>
        </location>
    </feature>
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        <location>
            <begin position="510"/>
            <end position="604"/>
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            <end position="76"/>
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        </location>
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        <location>
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            <end position="269"/>
        </location>
    </feature>
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            <begin position="275"/>
            <end position="292"/>
        </location>
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        <source>
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        <source>
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    </evidence>
    <sequence length="780" mass="83649" checksum="41F216DA899057AA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKW7</accession>
    <name>A0A4W2CKW7_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">C16H1orf159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSBIXG00000018626.1"/>
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    </dbReference>
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        <property type="entry name" value="DUF4501"/>
    </dbReference>
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        <property type="entry name" value="RIKEN CDNA 9430015G10 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIKEN CDNA 9430015G10 GENE"/>
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    </dbReference>
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        <property type="entry name" value="DUF4501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="383" mass="40090" checksum="10D35EC4ACE85D8E" modified="2019-09-18" version="1">MGSPHSCPLALARASGMDRCPLQAQLGQGQQPECCVDAGNINATCPGTSLCGPGCYGRPAEDGSVSCVQCRNGTHNSSECRGRTSFRPPPHPVLRPASPQGATPPSAGGKGGDPGCCPPSWDTVPPWSPESPPRVLMSLRAAVGQGLLCLHGHLPFSFLGHSCIWAASLAPRGPPAALTAVLPHSCRPRCTVPCEQERRDAWVAECWGPSGGRLPLPGDVSHQLGPHPLRGCILLPQASQQAAQGLLWKKQSSRPAAWRSRKYPMGRRRPCVCTSGCLSVWTGGRPAHRSHCAFSLFKAVMIPPPQSSGTSAPPTPHPIGCLVSGENFPFIPQMLSVSRVPNMGPESPLPPSALLQDPALPEPGAGGSHRLSYGPSRVTGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLI6</accession>
    <name>A0A4W2CLI6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLI6"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="524" mass="59964" checksum="DA561872A58120B8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMR7</accession>
    <name>A0A4W2CMR7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Intraflagellar transport protein 27 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Putative GTP-binding protein RAY-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Rab-like protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6. Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling. Forms a subcomplex within the IFT complex B with IFT25. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation. Plays a role in the early development of the kidney. May be involved in the regulation of ureteric bud initiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMR7"/>
    <dbReference type="STRING" id="30522.A0A4W2CMR7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025299.1">
        <property type="protein sequence ID" value="ENSBIXP00000014416.1"/>
        <property type="gene ID" value="ENSBIXG00000019128.1"/>
    </dbReference>
    <dbReference type="OMA" id="CFEISVK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001095">
        <property type="entry name" value="Intraflagellar transport protein 27 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="186" mass="20634" checksum="BDB7E3CA93D02DD9" modified="2019-09-18" version="1">MVKLAAKCILAGDPTVGKTALAQLFRSDGAHFQKNYSLTTGVDLVVKTVAVPDTGDSVELFIFDSAGKELFSEMLDKLWESPNVLCLVYDVTNEQSFTNCSKWLEKARSQIPGTTLPGVLVGNKTDLAGRRVVDVAQAQAWALGQGLECFETSVKEMENYEAPFHYLAKQFHHLYREKLEVFQVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMW6</accession>
    <name>A0A4W2CMW6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED19</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMW6"/>
    <dbReference type="STRING" id="30522.A0A4W2CMW6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023408.1">
        <property type="protein sequence ID" value="ENSBIXP00000013135.1"/>
        <property type="gene ID" value="ENSBIXG00000018097.1"/>
    </dbReference>
    <dbReference type="OMA" id="QRFCGSK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019403">
        <property type="entry name" value="Mediator_Med19_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536">
        <property type="entry name" value="LUNG CANCER METASTASIS-RELATED LCMR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10278">
        <property type="entry name" value="Med19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="260" mass="27943" checksum="2229D9375C4CF4E5" modified="2019-09-18" version="1">MRINARHRDGAGAEGTMENFTALFGAQADPPPPPAALGFGPGKPPPPPPPPPGGGPGPAPPPTAASAPPGSDKSAAGCGPFYLMRELPGSTELTGSTNLITHYNLEHAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILGGSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKQKHKQSRTQDPVPPETPSDSDHKKKKKKKEEDPERKRKKKEKKKKKNRHSPEHPGMGSSQASSSSSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CP62</accession>
    <name>A0A4W2CP62_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Formin-binding protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Formin-binding protein 30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FNBP4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CP62"/>
    <dbReference type="STRING" id="30522.A0A4W2CP62"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024306.1">
        <property type="protein sequence ID" value="ENSBIXP00000013744.1"/>
        <property type="gene ID" value="ENSBIXG00000018614.1"/>
    </dbReference>
    <dbReference type="OMA" id="QDGDGHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000056">
        <property type="entry name" value="Formin binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000058">
        <property type="entry name" value="Formin binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053076">
        <property type="entry name" value="WW_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46697">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46697:SF1">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="223"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="597"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="462"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="708"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="897"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="629"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="708"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="736"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="774"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="900"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="927"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="955"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00078190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1015" mass="109832" checksum="A828862C0943D91B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRY9</accession>
    <name>A0A4W2CRY9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Semaphorin-4F</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Semaphorin-W</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SEMA4F</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021696">
        <property type="term" value="P:cerebellar cortex morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061351">
        <property type="term" value="P:neural precursor cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040266">
        <property type="entry name" value="TRNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40714">
        <property type="entry name" value="TMF-REGULATED NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40714:SF1">
        <property type="entry name" value="TMF-REGULATED NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="336" mass="34473" checksum="A7F31F8E66307DAF" modified="2019-09-18" version="1">MWRRWMGTGCRWVEGPGDQKGSGSVLTGRGMGSGFRACTSLIGYAALRSAPCSIRRARGSPSASVGAAAASHLDLLRPSSPAWGEQPARPTDLQLRAAGGWGRGMPGCRISACGPGAQEGSAEPGSPPPPPPREPLSCPQPPSPSPTLTPTRAQASSQPGAAQESAGSAEGLELQRWRQGASGGAGGPGPAGGAGGGSGAAAAAAGVRALELAEARRRLLEVEGRRRLVSELESRVLQLHRVFLAAELRLAHRAESLGRLGGGVAQAELYLAAHGPRLKKGSRRVRRARPPALLASALGLGGCVPWGAGRLRRGHGPEPESPFRRSPPRGPASPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CV06</accession>
    <name>A0A4W2CV06_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DBH-like monooxygenase protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Monooxygenase X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MOXD1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the copper type II ascorbate-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027407372.1">
        <property type="nucleotide sequence ID" value="XM_027551571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CV06"/>
    <dbReference type="SMR" id="A0A4W2CV06"/>
    <dbReference type="STRING" id="30522.A0A4W2CV06"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027368.1">
        <property type="protein sequence ID" value="ENSBIXP00000015767.1"/>
        <property type="gene ID" value="ENSBIXG00000020142.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056753.1">
        <property type="protein sequence ID" value="ENSBIXP00005046900.1"/>
        <property type="gene ID" value="ENSBIXG00005031548.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898433"/>
    <dbReference type="CTD" id="26002"/>
    <dbReference type="GeneTree" id="ENSGT00530000063085"/>
    <dbReference type="OMA" id="MITGKHM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.310:FF:000002">
        <property type="entry name" value="DBH-like monooxygenase protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.230:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="entry name" value="Copper type II, ascorbate-dependent monooxygenase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014784">
        <property type="entry name" value="Cu2_ascorb_mOase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000323">
        <property type="entry name" value="Cu2_ascorb_mOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024548">
        <property type="entry name" value="Cu2_monoox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028460">
        <property type="entry name" value="Tbh/DBH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF28">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03712">
        <property type="entry name" value="Cu2_monoox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01082">
        <property type="entry name" value="Cu2_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00767">
        <property type="entry name" value="DBMONOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="PHM/PNGase F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0186">Copper</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610582" description="DBH-like monooxygenase protein 1" evidence="17">
        <location>
            <begin position="19"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="18">
        <location>
            <begin position="35"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="614" mass="69780" checksum="2EBCD3B5B55FD915" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CV43</accession>
    <name>A0A4W2CV43_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CV43"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027258.1">
        <property type="protein sequence ID" value="ENSBIXP00000015698.1"/>
        <property type="gene ID" value="ENSBIXG00000000478.1"/>
    </dbReference>
    <dbReference type="OMA" id="DNIRWIM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="269"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="486" mass="56697" checksum="3C646AA7B20754A3" modified="2019-09-18" version="1">MEKSTKTRPGADCGSDHELLITKFRLKLKKVGKTTRPFRYDLNQILYDYTVEVRNRFKGLDLIDRVPDELWKEVHDIVQETGIKTIPMEKKCRKAKWLSGEALQIAVKRIEVKSKGEKEKYKHLNAEFQRIARRDKKAFFSDQCKEIEENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHNGVITHLEPDILECEVQWALESITTNKASGGDAIPVELFQILKDDAVKVLHSICQQIWKTQQWPQNWKKSVFIPIPKKGNARECSQYRTIAFISHASKVMLKILQARLQQYVNHELPDVQASFRKGRGTRDQIDNIRWIMKKAREFQKNIYFCFTDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVITGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIARRNINNLRYADDTPLWQKVKRNSKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW56</accession>
    <name>A0A4W2CW56_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calretinin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CALB2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Calcium-binding protein involved in calcium homeostasis and signal transduction. It plays a critical role in buffering intracellular calcium levels and modulating calcium-dependent signaling pathways. Predominantly expressed in specific neuronal populations, influences synaptic plasticity and neuronal excitability, contributing to learning and memory. During embryonic development, it facilitates neuronal differentiation and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calbindin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371858.1">
        <property type="nucleotide sequence ID" value="XM_027516057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW56"/>
    <dbReference type="SMR" id="A0A4W2CW56"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019405.1">
        <property type="protein sequence ID" value="ENSBIXP00000010530.1"/>
        <property type="gene ID" value="ENSBIXG00000015996.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039805.1">
        <property type="protein sequence ID" value="ENSBIXP00005024534.1"/>
        <property type="gene ID" value="ENSBIXG00005027092.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876628"/>
    <dbReference type="CTD" id="794"/>
    <dbReference type="GeneTree" id="ENSGT00950000183108"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044293">
        <property type="term" value="C:dendriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098688">
        <property type="term" value="C:parallel fiber to Purkinje cell synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099534">
        <property type="term" value="F:calcium ion binding involved in regulation of presynaptic cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900271">
        <property type="term" value="P:regulation of long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16177">
        <property type="entry name" value="EFh_HEF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000054">
        <property type="entry name" value="Calbindin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000165">
        <property type="entry name" value="Calbindin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000116">
        <property type="entry name" value="calretinin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029646">
        <property type="entry name" value="CALB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF4">
        <property type="entry name" value="CALRETININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="271" mass="31351" checksum="3051D4674E17CD7E" modified="2019-09-18" version="1">MAGPQQQPPYLHLAELTATQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMVSKSDNLGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTSYRKSVMSLAEAGKLYRKDLEIVLCSEPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW76</accession>
    <name>A0A4W2CW76_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HYDIN/VesB/CFA65-like Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW76"/>
    <dbReference type="SMR" id="A0A4W2CW76"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044688.1">
        <property type="protein sequence ID" value="ENSBIXP00000010553.1"/>
        <property type="gene ID" value="ENSBIXG00000016008.1"/>
    </dbReference>
    <dbReference type="OMA" id="AMSEVNI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904158">
        <property type="term" value="P:axonemal central apparatus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033305">
        <property type="entry name" value="Hydin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF012200">
        <property type="entry name" value="choice_anch_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053">
        <property type="entry name" value="DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053:SF0">
        <property type="entry name" value="HYDROCEPHALUS-INDUCING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14874">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="7">
        <location>
            <begin position="198"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="7">
        <location>
            <begin position="459"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="957"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114147" checksum="0CAEC15FC6A0DDF0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXT2</accession>
    <name>A0A4W2CXT2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kynurenine--oxoglutarate transaminase 3</fullName>
            <ecNumber evidence="6">2.6.1.63</ecNumber>
            <ecNumber evidence="5">2.6.1.7</ecNumber>
            <ecNumber evidence="4">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cysteine-S-conjugate beta-lyase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Kynurenine aminotransferase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Kynurenine aminotransferase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Kynurenine--glyoxylate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Kynurenine--oxoglutarate transaminase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">KYAT3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others. May catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond. Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-hydroxy-L-kynurenine + glyoxylate = xanthurenate + glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:65900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:71201"/>
            <dbReference type="EC" id="2.6.1.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:65901"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-kynurenine + 2-oxoglutarate = kynurenate + L-glutamate + H2O</text>
            <dbReference type="Rhea" id="RHEA:65560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="EC" id="2.6.1.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:65561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-kynurenine + glyoxylate = kynurenate + glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:65896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="EC" id="2.6.1.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:65897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18121"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="EC" id="4.4.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:18122"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.63" evidence="6"/>
    <dbReference type="EC" id="2.6.1.7" evidence="5"/>
    <dbReference type="EC" id="4.4.1.13" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXT2"/>
    <dbReference type="SMR" id="A0A4W2CXT2"/>
    <dbReference type="STRING" id="30522.A0A4W2CXT2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031227.1">
        <property type="protein sequence ID" value="ENSBIXP00000018286.1"/>
        <property type="gene ID" value="ENSBIXG00000022146.1"/>
    </dbReference>
    <dbReference type="OMA" id="PRDFKLC"/>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00726"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047315">
        <property type="term" value="F:kynurenine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000021">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF6">
        <property type="entry name" value="KYNURENINE--OXOGLUTARATE TRANSAMINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="23">
        <location>
            <begin position="64"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00054518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="455" mass="51472" checksum="E353F80DCD854BD6" modified="2019-09-18" version="1">MFLAWERLCTLSCRPKFLKTVWASKILGLSTSAKMSLRFKNAKRIEGLDSNIWIEFTKLAADPSVVNLGQGLPDISPPVYVKEELSKIAAIDNLNQYTRGFGHPSLVKALSCLYEKFYHNKINPNEEILVTVGAYGSLFNAIQGLIDEGDEVIVIVPFFDCYESMVRMAGATPVFVPLRCKPVDGKKCSSSDWTLDPQELASKFNSKTKAIILNTPHNPLGKVYTKEELQVIADLCIKYDTLCISDEVYEWLVYTGNKHFKIATFPGMWERTITIGSAGKTFSVTGWKLGWSIGPKHLIKHLQTVQQNTVYTCATPLQEALAQAFWIDIKRMDDPECYFNSLPKELEVKRDRMVHLLESVGLKSIVPDGGYFIIADVSLLDVDLLDMKDSNEPYDYKFVKWMIKNKKLSAIPVSAFCNAETKSQFEKFVRFCFIKKDSTLDAAEEIIKAWSRQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1B3</accession>
    <name>A0A4W2D1B3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1B3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033217.1">
        <property type="protein sequence ID" value="ENSBIXP00000019551.1"/>
        <property type="gene ID" value="ENSBIXG00000023139.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="20" mass="2195" checksum="C839789A3E9968EF" modified="2019-09-18" version="1">DYGNRLTFGNGTRVLVTPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2Q4</accession>
    <name>A0A4W2D2Q4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMAGP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2Q4"/>
    <dbReference type="STRING" id="30522.A0A4W2D2Q4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031584.1">
        <property type="protein sequence ID" value="ENSBIXP00000018492.1"/>
        <property type="gene ID" value="ENSBIXG00000004326.1"/>
    </dbReference>
    <dbReference type="OMA" id="QMEDFPH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043243">
        <property type="entry name" value="SMAGP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47394">
        <property type="entry name" value="SMALL CELL ADHESION GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47394:SF1">
        <property type="entry name" value="SMALL CELL ADHESION GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="97" mass="10565" checksum="1D17AA00C510FBA9" modified="2019-09-18" version="1">MTSFPTTPPPAEELMATTILQATEALSPEAEASTALIAVVITVVFLTLLSVVILIFFYLYKNKGSYVTYEPADGEPGAVVLMENDSAKGREKEEYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3V2</accession>
    <name>A0A4W2D3V2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC113897870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3V2"/>
    <dbReference type="STRING" id="30522.A0A4W2D3V2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034632.1">
        <property type="protein sequence ID" value="ENSBIXP00000020507.1"/>
        <property type="gene ID" value="ENSBIXG00000023819.1"/>
    </dbReference>
    <dbReference type="OMA" id="GMKKGWQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042641">
        <property type="term" value="C:actomyosin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057529">
        <property type="entry name" value="MRCK/ROCK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF31">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MRCK ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25346">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="214"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="360" mass="40570" checksum="157A7E8CCE3F02A7" modified="2019-09-18" version="1">MSKDRTSLFYRLNDYKPLKNASGSIKDEKEAQDLVLEKTEEEESLRNSSLVLGARKMPGAMERCDKLDMSAMIMLESALDREKISRKILPEAVPVTLNCKMKAREKYQSDMWLEIEHPNKGLVLQDPSSSSLALFNTTSNAVAQCEGDPVDNIFEQNLKIKFYISSRSTSPSSYSEDEADTVKITDSAHLPVHTPTIKRKECHSRIFFPTKHKAHRFFLTSFTAPTKCHQCTSLMVGLIRQGCTCDACGFSCHTACVEKAPRACPVPFHQKKGAFCLDPQKGLGTVYEGHVWIPKKAGMKKGWQRALAIVCDFKLFLFDMNEEDSKPNVVVSQVIDMRVLLFLQGWSVFCQFKKGNASYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5V1</accession>
    <name>A0A4W2D5V1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5V1"/>
    <dbReference type="STRING" id="30522.A0A4W2D5V1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033279.1">
        <property type="protein sequence ID" value="ENSBIXP00000019598.1"/>
        <property type="gene ID" value="ENSBIXG00000023184.1"/>
    </dbReference>
    <dbReference type="OMA" id="VIYTAEM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B17.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="220" mass="25453" checksum="300B8D9364EDBBBA" modified="2019-09-18" version="1">MASLKTQLWRDNKAHLLCKTGGGHKGELQTRACAKPTPPAARAWKLSVVGSLPLSGWFRRWVFRGAPERRGLQDKMELLQVLKRGLQQVSGHGGLRGYLRVLFRANDVRVGTLVGEDKYGNKYYEDNKQFFGRHRWVIYTTEMNGKNTFWDVDGSMVPPEWHRWLHCMTDDPPTVKPPTARKFIWTNHKFNLSGTPQQYVPYSTTRKKIQEWVPPSTPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D621</accession>
    <name>A0A4W2D621_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FERM domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FRMD3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Putative tumor suppressor gene that may be implicated in the origin and progression of lung cancer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D621"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035958.1">
        <property type="protein sequence ID" value="ENSBIXP00000021330.1"/>
        <property type="gene ID" value="ENSBIXG00000007495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:actomyosin structure organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FERM_B-lobe"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="FERM_3"/>
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    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="527"/>
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        <location>
            <begin position="32"/>
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            <begin position="344"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="435"/>
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        <location>
            <begin position="344"/>
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        <location>
            <begin position="358"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00058056"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00067910"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021330.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="555" mass="63703" checksum="B98E6731969D311D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7T4</accession>
    <name>A0A4W2D7T4_BOBOX</name>
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        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021961.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="303" mass="33445" checksum="F4FD190EBF0A60D4" modified="2025-10-08" version="2">MTMPLQEFVLDGFQTQALLFSLFLALYVVAVLGNLTMIVVITLDAHLHSPMYFFLKNLSFVDLCYSSVIYPKALANFLSSSKVITFAGCATQFFFFSMVGTTEAFLLAVMAYDRFVAICSPLCYPISMRPSVCARLVLGTYCGGCVNSILQTSFTFSLQFCSSNHIDHFFCDVPPLLKLACADTTINELVMFGLCGVIIVGTILMILISYGYITVTILKMRSGGGRHKLFSTCGSHLTAVSIYYGTVFVMYAQPGAVESVEQGMVVSVFYTLVIPMLNPLIYSLRNKEVKDALWRLGQKHTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7Y2</accession>
    <name>A0A4W2D7Y2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">ADAM metallopeptidase domain 28</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7Y2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036933.1">
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        <property type="gene ID" value="ENSBIXG00000006735.1"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="F:metalloendopeptidase activity"/>
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    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
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        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DISIN"/>
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        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021269602" description="ADAM metallopeptidase domain 28" evidence="15">
        <location>
            <begin position="21"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="664"/>
            <end position="688"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="18">
        <location>
            <begin position="205"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="17">
        <location>
            <begin position="409"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="627"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="18">
        <location>
            <begin position="971"/>
            <end position="1166"/>
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    </feature>
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        <location>
            <begin position="1173"/>
            <end position="1214"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="707"/>
            <end position="736"/>
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        <location>
            <position position="342"/>
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    <feature type="active site" evidence="12">
        <location>
            <position position="1107"/>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="341"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="345"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="351"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <begin position="316"/>
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    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="356"/>
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    <feature type="disulfide bond" evidence="12">
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            <begin position="467"/>
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        <location>
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        <location>
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            <begin position="1123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50215"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1219" mass="137413" checksum="E1C7A24AC7206632" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9D0</accession>
    <name>A0A4W2D9D0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Torsin</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homohexamer. Interacts with TOR1B; the interaction may be specific of neural tissues. Interacts (ATP-bound) with TOR1AIP1 and TOR1AIP2; the interactions induce ATPase activity. Interacts with KLHL14; preferentially when ATP-free. Interacts with KLC1 (via TPR repeats); the interaction associates TOR1A with the kinesin oligomeric complex. Interacts with COPS4; the interaction associates TOR1A with the CSN complex. Interacts with SNAPIN; the interaction is direct and associates SNAPIN with the CSN complex. Interacts with STON2. Interacts (ATP-bound) with SYNE3 (via KASH domain); the interaction is required for SYNE3 nuclear envelope localization. Interacts with VIM; the interaction associates TOR1A with the cytoskeleton. Interacts with PLEC. Interacts (ATP-bound) with SLC6A3; regulates SLC6A3 transport to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 18">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the ClpA/ClpB family. Torsin subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000015358.1"/>
        <property type="gene ID" value="ENSBIXG00000019892.1"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0045202">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049337">
        <property type="entry name" value="TOR1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
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    <dbReference type="InterPro" id="IPR017378">
        <property type="entry name" value="Torsin_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF15">
        <property type="entry name" value="TORSIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21376">
        <property type="entry name" value="TOR1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06309">
        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038079">
        <property type="entry name" value="Torsin_2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021443807" description="Torsin" evidence="20">
        <location>
            <begin position="21"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Torsin-1A C-terminal" evidence="21">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="103"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR038079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038079-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="326" mass="36719" checksum="E7D9DB875486DFC5" modified="2019-09-18" version="1">MKLGRAALGLLLLAPLAVRAVEPISLGLALAGVLTGYISYPRLYCLFAECCSPKRSLSREALQKDLDSKLFGQHLAKKVILNALSGFISNPKPKKPLTLSLHGWTGTGKNFVSKIIAENIYEGGLNSDYVHLFVATLHFPHISNITLYKDQLQSWIRGNVSACARSIFIFDEMDKMHAGLIDAIKPYLDYYDNLDGVSYQKAIFIFLSNAGAERITDVALDFWRSGKQREEIKLKDMEHALSVSAFNNKNSGFWHSSLIDRNLIDYFVPFLPLEYKHLKMCIRVEMQSRGYEVDEDIVSRVAEEMTFSPKRREFSPTKAVKPCSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBE0</accession>
    <name>A0A4W2DBE0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger and BTB domain-containing protein 8B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBE0"/>
    <dbReference type="STRING" id="30522.A0A4W2DBE0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027849.1">
        <property type="protein sequence ID" value="ENSBIXP00000016086.1"/>
        <property type="gene ID" value="ENSBIXG00000020379.1"/>
    </dbReference>
    <dbReference type="OMA" id="MMDVQTD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18330">
        <property type="entry name" value="BTB_POZ_ZBTB8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000379">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000112">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF25">
        <property type="entry name" value="ZGC:110075 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="365"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="157"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="444"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="463"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="491" mass="53835" checksum="9ED23921E9A48BD8" modified="2019-09-18" version="1">MEMQSYYAKLLGELNEQRQRDFFCDCSIIVEGRIFKAHRNILFANSGYFRALLIHYIQDSGRHSTASLDIVTSDAFSTILDFLYSGKLDLCGENVIEVMSAASYLQMNDVVNFCKTYIRSSLDICRKMEKEAAVAAAVAAAAAAAAAAAAAAAAHQMDSGSPKKGTAGETKSLVSRAEGEESEDRPREPPSGDLRGCCPLELVGKDSQGSGWVDSDRSTRPEQMEPKVELGAAEVEVDGQLQPPATPLSLAHREEGLPSSQAIDLAYSNYHVKQFLEALLRNSAVQSKEDVDHHFSQSLEGRPGGAGVAAMSSTMDVQTDWYGEDSGDVLVVPIKLHKCPFCPYSAKQKGILKRHIRSHTGERPYPCETCGKRFTRQEHLRSHALSVHRSNRPIICKGCRRTFTSHLSPGLRRFGLCDSCTCITDTHDDDDELMPINLSLVEASFESQEKSDTDSDWPIYVESGEENDPTGEDSDDRPQIQPNLSGGDTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCN7</accession>
    <name>A0A4W2DCN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028502.1">
        <property type="protein sequence ID" value="ENSBIXP00000016509.1"/>
        <property type="gene ID" value="ENSBIXG00000003830.1"/>
    </dbReference>
    <dbReference type="OMA" id="FCMEGNS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
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    </dbReference>
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        <property type="entry name" value="RT_POL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="17"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="472" mass="54550" checksum="034A66F753696D20" modified="2019-09-18" version="1">MNRTNLFCMEGNSICQQIWKTQQWPQDWKRSVFIPLPKKGNAKGCLNYRTIALISHASKVMLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANIHWIMEKAREFQKNIYFCFIDYAKAFDCVDHNQLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNDGLGETQVRIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESKKVGLKLNIQKTKIMASGPTTSWKIDGETVETVSDFILLGSKITTDGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITWPTKVRLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMILKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDMSLSELWELVMDREAWCAVIHGVAKSQTQLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DD61</accession>
    <name>A0A4W2DD61_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">RE1-silencing transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Neural-restrictive silencer factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC113894322</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DD61"/>
    <dbReference type="STRING" id="30522.A0A4W2DD61"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037282.1">
        <property type="protein sequence ID" value="ENSBIXP00000022241.1"/>
        <property type="gene ID" value="ENSBIXG00000025177.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045665">
        <property type="term" value="P:negative regulation of neuron differentiation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="RE1-silencing transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RE1-silencing transcription factor A"/>
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        <property type="entry name" value="RE1-silencing transcription factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RE1-silencing transcription factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RE1-silencing transcription factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057281">
        <property type="entry name" value="Zfn-C2H2_REST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF102">
        <property type="entry name" value="RE1-SILENCING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24540">
        <property type="entry name" value="zf-C2H2_REST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="331"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
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            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
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        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="423"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="761"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="821"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="432"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="614"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="640"/>
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        </location>
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        <location>
            <begin position="665"/>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
            <begin position="777"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="892"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1004" mass="110825" checksum="D1B6F8CDF84DE814" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEE6</accession>
    <name>A0A4W2DEE6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Usherin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Interacts with collagen IV and fibronectin via its laminin EGF-like domains. Interaction with collagen may be required for stable integration into the basement membrane. Interacts with NINL. Interacts with USH1C. Component of USH2 complex, composed of ADGRV1, PDZD7, USH2A and WHRN. Interacts with ADGRV1/MASS1 (via N-terminal PDZ domain). Interacts (via the cytoplasmic region) with WHRN. Interacts (via the cytoplasmic region) with PDZD7. Interacts (via the cytoplasmic region) with VEZT and MYO7A (via MyTH4-FERM domains); the interaction associates VEZT with the USH2 complex at the stereocilia base.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell projection</location>
            <location evidence="16">Stereocilium membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000017171.1"/>
        <property type="gene ID" value="ENSBIXG00000004020.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060171">
        <property type="term" value="C:stereocilium membrane"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1952"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="749"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="870"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="922"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="934"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="951"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="953"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="1002"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="1004"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="5204" mass="573986" checksum="37F941D3A65B1F13" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFH3</accession>
    <name>A0A4W2DFH3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ubiquitin carboxyl-terminal hydrolase 48</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Deubiquitinating enzyme 48</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin thioesterase 48</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitin-specific-processing protease 48</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with TRAF2 and RELA. Interacts with GPS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFH3"/>
    <dbReference type="STRING" id="30522.A0A4W2DFH3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050713.1">
        <property type="protein sequence ID" value="ENSBIXP00000022906.1"/>
        <property type="gene ID" value="ENSBIXG00000002098.1"/>
    </dbReference>
    <dbReference type="OMA" id="YLGQEKW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02668">
        <property type="entry name" value="Peptidase_C19L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01795">
        <property type="entry name" value="Ubl_USP48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000029">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 48 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033841">
        <property type="entry name" value="Pep_USP48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044743">
        <property type="entry name" value="Ubl_USP48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF722">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="20">
        <location>
            <begin position="89"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="21">
        <location>
            <begin position="460"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="19">
        <location>
            <begin position="929"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="611"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="880"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="895"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="907"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1071" mass="123388" checksum="40A1DD2021DC231C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGV0</accession>
    <name>A0A4W2DGV0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine-rich repeat-containing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LRRC41</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027393184.1">
        <property type="nucleotide sequence ID" value="XM_027537383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGV0"/>
    <dbReference type="SMR" id="A0A4W2DGV0"/>
    <dbReference type="STRING" id="30522.A0A4W2DGV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039198.1">
        <property type="protein sequence ID" value="ENSBIXP00000023570.1"/>
        <property type="gene ID" value="ENSBIXG00000026035.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005002.1">
        <property type="protein sequence ID" value="ENSBIXP00005031342.1"/>
        <property type="gene ID" value="ENSBIXG00005001150.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889944"/>
    <dbReference type="CTD" id="10489"/>
    <dbReference type="GeneTree" id="ENSGT00390000015908"/>
    <dbReference type="OMA" id="VVSDSWH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000114">
        <property type="entry name" value="leucine-rich repeat-containing protein 41 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000088">
        <property type="entry name" value="Putative leucine-rich repeat-containing protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026137">
        <property type="entry name" value="Leu_rpt_41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15354:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15354">
        <property type="entry name" value="MUF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="265"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="814" mass="88677" checksum="60608574DFB79A03" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJ24</accession>
    <name>A0A4W2DJ24_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cellular retinoic acid binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CRABP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027385750.1">
        <property type="nucleotide sequence ID" value="XM_027529949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJ24"/>
    <dbReference type="SMR" id="A0A4W2DJ24"/>
    <dbReference type="STRING" id="30522.A0A4W2DJ24"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051493.1">
        <property type="protein sequence ID" value="ENSBIXP00000024304.1"/>
        <property type="gene ID" value="ENSBIXG00000006143.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017060.1">
        <property type="protein sequence ID" value="ENSBIXP00005009201.1"/>
        <property type="gene ID" value="ENSBIXG00005014157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884881"/>
    <dbReference type="CTD" id="1382"/>
    <dbReference type="GeneTree" id="ENSGT00940000157619"/>
    <dbReference type="OMA" id="PVWETDN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001972">
        <property type="term" value="F:retinoic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035115">
        <property type="term" value="P:embryonic forelimb morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048672">
        <property type="term" value="P:positive regulation of collateral sprouting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042573">
        <property type="term" value="P:retinoic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19461">
        <property type="entry name" value="CRABP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="138" mass="15749" checksum="34A4D5430775A0E3" modified="2019-09-18" version="1">MPNFSGNWKIIRSENFEDLLKVLGVNVMLRKIAVAAASKPAVEIKQEGDTFYIKTSTTVRTTEINFKIGEEFEEQTVDGRPCKSLVKWESENKMVCEQRLLKGEGPKTSWTRELTNDGELILTMTADDIVCTRVYIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKA9</accession>
    <name>A0A4W2DKA9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="13">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TRMT13</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKA9"/>
    <dbReference type="STRING" id="30522.A0A4W2DKA9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048902.1">
        <property type="protein sequence ID" value="ENSBIXP00000019339.1"/>
        <property type="gene ID" value="ENSBIXG00000002727.1"/>
    </dbReference>
    <dbReference type="OMA" id="HRCSWRS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="15">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="381"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="481" mass="54041" checksum="EBA62A433336F9FF" modified="2019-09-18" version="1">MAAAALTPHGTVFPEEGRCGYFVEKKKRFCRMVVAAGKRFCGEHAGASEEENARKRILCPLDPKHTVYEDRLAKHLKKCNSREKPKPDFFIQDINAGLKDETEIPDQLVPISSLSEEQLENLIKKLRKASEGLNCTLKDQIMSHPALHDALNDPKNGDSAAKHLKQQASILGNIENLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKVHFILVEKMTTRFKVDGKHRKKNSVFERLQIDIQHLCLNKIPLLSKEKLPIVGIGKHLCGVATDLALRCLVETYAASCEERNEEPLAKRIKNDKTEKEINTLAKEGNEKNIPEKWTPVAGIVIALCCHHRCDWRHYVGKEYFRALGLGAVDFHYFQRMSSWATCGMRKTTLEASNSSTKRRDKQNDGSEEHDDGGCRITDDSPESLPGFLTGEEKKKIGHLCKLLIDQGRIKYLQQKGFSPALQYYTDPLVSLENVLLTALPNHSSSPETSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMN1</accession>
    <name>A0A4W2DMN1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMN1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012899.1">
        <property type="protein sequence ID" value="ENSBIXP00000027595.1"/>
        <property type="gene ID" value="ENSBIXG00000008479.1"/>
    </dbReference>
    <dbReference type="OMA" id="TELNCMF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="117"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="298"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="577" mass="66645" checksum="84E346B5BB435FBB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNK9</accession>
    <name>A0A4W2DNK9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">SRSF protein kinase 2</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">SFRS protein kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Serine/arginine-rich protein-specific kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SRPK2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs). Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Interacts with ACIN1. Interacts with POLR2A/RNA polymerase II; the interaction occurs during the co-transcriptional formation of inappropriate R-loops.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNK9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042936.1">
        <property type="protein sequence ID" value="ENSBIXP00000025989.1"/>
        <property type="gene ID" value="ENSBIXG00000005899.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040647.1">
        <property type="protein sequence ID" value="ENSBIXP00005043852.1"/>
        <property type="gene ID" value="ENSBIXG00005006954.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154795"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000105">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000834">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000163">
        <property type="entry name" value="SRSF protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF6">
        <property type="entry name" value="SRSF PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="17" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="79"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="329"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="462"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="26">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="26">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00076949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="686" mass="77442" checksum="00D8350247090491" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUU2</accession>
    <name>A0A4W2DUU2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ras-related protein Rab-4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cell membrane</location>
            <topology evidence="17">Lipid-anchor</topology>
            <orientation evidence="17">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUU2"/>
    <dbReference type="STRING" id="30522.A0A4W2DUU2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004087.1">
        <property type="protein sequence ID" value="ENSBIXP00000030421.1"/>
        <property type="gene ID" value="ENSBIXG00000012395.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04113">
        <property type="entry name" value="Rab4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000280">
        <property type="entry name" value="Putative ras-related protein Rab-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041819">
        <property type="entry name" value="Rab4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="12 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 17" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11 17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="60"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="102"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="456" mass="48554" checksum="F86020D99163EE4B" modified="2019-09-18" version="1">RRPSLCGGGPPGKGRPSSPRRALSLPCEGADPAGSAPDTPSPFCHLRACGPPASPDPCRHCRGRAPPRAPGLATPLAGPAPAAPCARPQRGLGLAPPLTLRPCPPLRPCPPLRPSPPLRPCPSPTPLPLPSAPAPPLRPCPSPPPLPLPSAPAPPLRPCPSPRRPDRRGEAVRLRGARSPAGPQQLGAGSCLGDRGPLPPPLPCPSRTPPRRGRLPGLPAAGSAEPQRRPARGGAAPKMSQTAMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDTDREVTFLEASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRAQAPSAQECGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUW5</accession>
    <name>A0A4W2DUW5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">sphingomyelin phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1-O-octadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-octadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74001"/>
            <dbReference type="ChEBI" id="CHEBI:75216"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:39924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:41120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a sphingosylphosphocholine + H2O = a sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:84410"/>
            <dbReference type="ChEBI" id="CHEBI:85171"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an N-(acyl)-sphingosylphosphocholine + H2O = an N-acyl-sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45301"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUW5"/>
    <dbReference type="STRING" id="30522.A0A4W2DUW5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004006.1">
        <property type="protein sequence ID" value="ENSBIXP00000030446.1"/>
        <property type="gene ID" value="ENSBIXG00000012428.1"/>
    </dbReference>
    <dbReference type="OMA" id="LGCWAPA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006684">
        <property type="term" value="P:sphingomyelin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF9">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="188"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="473" mass="51906" checksum="C5078E626E2D9C93" modified="2019-09-18" version="1">MPSPPDWPPTPCALRPSPFPHPVLHALHRLTRALLFPAYWALDQLLGCWAPAESRSEQSWLRTAAGAGGALLLLLAALPLTLPALLLWLLLQAWRRPFCYQPPPRCWAPPAPWCPRTEPARSFGFFSANLCLLPDGLARFNNLPHTQRRAAAVGAVLLAGLRRSPYGATGCGSPVQAMPCGVLTGAMPASLDFVCLQEAFDLRAERRLVSLLASNLGPVLYDVGTFGLQPGLHLKLLGSGLLLASRYPLLRAAFQSFPHARGEDALASKGLLSAQAQLGILDGRRVVGFLHCTHLHAPSEDGLLRCKQLTVLLDWAEQFEAESRQSDEAVAFSVLLGDLNFDNCSLDHQQEQEHRFFSCFRDPCRLGTRREQPWALGTLLRPSELRRSVACSPEMLRRALEQKKGRRRYLAGPPRGGPPAESWRGRRLDYITYRSAPGGLLSPEVEQVTFSTALAGLTDHLAVGLRLRVSTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWF2</accession>
    <name>A0A4W2DWF2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWF2"/>
    <dbReference type="STRING" id="30522.A0A4W2DWF2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003368.1">
        <property type="protein sequence ID" value="ENSBIXP00000030675.1"/>
        <property type="gene ID" value="ENSBIXG00000012727.1"/>
    </dbReference>
    <dbReference type="OMA" id="GSWRCCS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031438">
        <property type="entry name" value="MCCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37877">
        <property type="entry name" value="MITOCHONDRIAL COILED-COIL DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37877:SF1">
        <property type="entry name" value="MITOCHONDRIAL COILED-COIL DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15707">
        <property type="entry name" value="MCCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021452452" evidence="2">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="113" mass="12819" checksum="EF70F6E71CD22527" modified="2019-09-18" version="1">MGLPLAWLSQCCRHLLLPSWAPILQGSWRCCSQGPRSTAETSSTRNRKMSPSRERQPRPTAELAQAEELLEQQLELYQALVEGQEGAWEAQALVLKTQKLKEQMRRESLGEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXE6</accession>
    <name>A0A4W2DXE6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="6">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">DPAGT1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027419318.1">
        <property type="nucleotide sequence ID" value="XM_027563517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXE6"/>
    <dbReference type="SMR" id="A0A4W2DXE6"/>
    <dbReference type="STRING" id="30522.A0A4W2DXE6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009660.1">
        <property type="protein sequence ID" value="ENSBIXP00000028654.1"/>
        <property type="gene ID" value="ENSBIXG00000007133.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053150.1">
        <property type="protein sequence ID" value="ENSBIXP00005044288.1"/>
        <property type="gene ID" value="ENSBIXG00005004837.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905766"/>
    <dbReference type="CTD" id="1798"/>
    <dbReference type="GeneTree" id="ENSGT00390000011424"/>
    <dbReference type="OMA" id="LPHFNAR"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003976">
        <property type="term" value="F:UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048439">
        <property type="entry name" value="DPAGT1_ins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21383">
        <property type="entry name" value="DPAGT1_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="DPAGT1 insertion" evidence="22">
        <location>
            <begin position="428"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="518" mass="58394" checksum="B3298085EF850755" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZV8</accession>
    <name>A0A4W2DZV8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">POLR1C</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively. POLR1C/RPAC1 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZV8"/>
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    <dbReference type="STRING" id="30522.A0A4W2DZV8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007264.1">
        <property type="protein sequence ID" value="ENSBIXP00000029443.1"/>
        <property type="gene ID" value="ENSBIXG00000000753.1"/>
    </dbReference>
    <dbReference type="OMA" id="KKKCRAF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000003">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000005">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000009">
        <property type="entry name" value="RNA polymerase I and III subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="11">
        <location>
            <begin position="60"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="346" mass="39116" checksum="DB5EA01B8886F738" modified="2019-09-18" version="1">MAAAQAVEEMRTRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEGGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHRVYTRHMTWVPLGNQADLFPEGAIRPVHDDILIAQLRPGQEIDLLMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSRCFSPGVIEVQEIQGKKVARVANPRLDTFSREVFRNEKLKKVVRLARVRDHFIFSVESTGVLPPDVLVSEAIKVLMGKCQRFLDELDAVQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1W7</accession>
    <name>A0A4W2E1W7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NOL12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Multifunctional RNA binding protein that plays a role in RNA metabolism and DNA maintenance. Participates in the resolution of DNA stress and the maintenance of genome integrity by localizing to sites of DNA insults. Also plays a role in proper nucleolar organization by limiting nucleolar size and regulating nucleolar number. Mechanistically, regulates the nucleolar levels of fibrillarin and nucleolin, two key players in pre-rRNA processing and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with KIAA1191.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E1W7"/>
    <dbReference type="SMR" id="A0A4W2E1W7"/>
    <dbReference type="STRING" id="30522.A0A4W2E1W7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003087.1">
        <property type="protein sequence ID" value="ENSBIXP00000030771.1"/>
        <property type="gene ID" value="ENSBIXG00000012853.1"/>
    </dbReference>
    <dbReference type="OMA" id="LHMHSRK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="35"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="213" mass="24707" checksum="2E186D2BA25E044A" modified="2019-09-18" version="1">MGRNKKKRDGDDRRQRLILSFDEEKRREYLTGFHKRKVERKKAAIEEIKQRLKEEQKKLREERHQEYLKMLAEREEALEEADELDRLVTAKTESVQYDHPNHTVTVTTISDLDLSGARLLGLPTPEQGAGSGPEEEVSSMEKPTRALPRKSRDPLLSQRISSLTASLHAHSRKKVKKKRLRRAQDTTKKPPSATRTSKTRRRRLTGKARHSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E315</accession>
    <name>A0A4W2E315_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kelch-like ECH-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the KEAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E315"/>
    <dbReference type="STRING" id="30522.A0A4W2E315"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042964.1">
        <property type="protein sequence ID" value="ENSBIXP00000026009.1"/>
        <property type="gene ID" value="ENSBIXG00000028096.1"/>
    </dbReference>
    <dbReference type="OMA" id="TECLTEY"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18458">
        <property type="entry name" value="BACK_KLHL19_KEAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18248">
        <property type="entry name" value="BTB_POZ_KLHL19_KEAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000024">
        <property type="entry name" value="Kelch-like ECH-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047098">
        <property type="entry name" value="KEAP1_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030563">
        <property type="entry name" value="KEAP1_BTB_POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF162">
        <property type="entry name" value="KELCH-LIKE ECH-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="77"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="623" mass="69609" checksum="C802080D05624545" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6B0</accession>
    <name>A0A4W2E6B0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6B0"/>
    <dbReference type="STRING" id="30522.A0A4W2E6B0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045565.1">
        <property type="protein sequence ID" value="ENSBIXP00000034496.1"/>
        <property type="gene ID" value="ENSBIXG00000002927.1"/>
    </dbReference>
    <dbReference type="OMA" id="WTYFTHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd09851">
        <property type="entry name" value="HTLV-1-like_HR1-HR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424:SF73">
        <property type="entry name" value="ENDOGENOUS RETROVIRUS GROUP FC1 ENV POLYPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424">
        <property type="entry name" value="VIRAL ENVELOPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00429">
        <property type="entry name" value="TLV_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034496.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="510" mass="56659" checksum="B0BEC2ABC0117D8F" modified="2019-09-18" version="1">MCSTPRVLFPLHCLNNSPMTPLFLILAALPSLLATPCPCSDIYSCVHSSCYNTAPKPCTMNLRGGKSKSLLTVRVQKRGSFMSTCHKPNGRNICFYPITYWGYSDGGRVLDQNREKKQEQVIKNIISQLQATPEPYKCLDLLSQLWPLLKSPSGNQHLTRLLNSSFQFFQCTQHTSQCWICMSLSPSPHIAIPLPSIWLSQGNYTSPSQQKTAQLIGPVSDNVLLSASSNLTCINYSSPNYTGLTNSAPSFFSTCVHSTNNCTNLEIFILCGTYAYSCLPLDPPGTCILVFLTPGLTFLKEEVEQIVYPQGELSHRKRSAVIAPLLIGTGLAVALGTGIGGISTSVHFYYKLSQEPNEDVEQVVESFVSIQRQINSLASVALQNTRALDLLTAEKGGTCLFLGEDCCYFVNEMGTVQGRVKELRDRIECRRKELQNLYSPQNLFQQALPWLLPFLGPLVLIILFLLFGHCLFNLFQRFLQERIQAISRDYVKTILLLESLIPSSEKGDPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8D5</accession>
    <name>A0A4W2E8D5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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            <begin position="182"/>
            <end position="450"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033079.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="632" mass="72931" checksum="3D5F0EA57749959D" modified="2019-09-18" version="1"> </sequence>
</entry>
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    <name>A0A4W2E8Z6_BOBOX</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
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    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sperm_Mat_Cystatin"/>
    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="141" mass="16355" checksum="9BD37057E796AA84" modified="2019-09-18" version="1">MARICPGLLLWMTVAALASRGVQAWDSKKVTREFQDIPASYVYVQQALWFAMKEYNKASKDTYVYKVMNVLRSQEQVTDSLDYLLEVEIERTQCKKISGKTENCSAQEDPQMQKAFYCTFIVASKPWKFELQMLKKECSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB27</accession>
    <name>A0A4W2EB27_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative pheromone receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EB27"/>
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        <property type="protein sequence ID" value="ENSBIXP00000034091.1"/>
        <property type="gene ID" value="ENSBIXG00000017023.1"/>
    </dbReference>
    <dbReference type="OMA" id="CKFFIFL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016503">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13949">
        <property type="entry name" value="7tm_V1R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01534">
        <property type="entry name" value="VOMERONASL1R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35323" checksum="AA1A0DC894B37520" modified="2019-09-18" version="1">IPVLSHSHLHDIKLSWAGSCHMPYSAASMIILTQTVVGILGNFSLLCSYIVLHIMGYRLRSTDLILKHLIVANSLVLLCKGVPETMAIFGWKQIRSDFGCKLLFFLHRMGRGMSIGSICLLSVFQMITISPWNSRWAALKVIAPKYTVPSIFLCWILQMPVNVIFPIFITGKWSHNNITEERDYGCCSSIQTDQKNIKTRDALYAALLSSPDVLCLGLTLWAGGSMVLILYRHKQQVQHIRRTDASSRSSPESRATKTILLLGPSWLFVNMTIIIAACFPSVSPFLLISRDSSVQRLYFAWERNAKSSTIMRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB60</accession>
    <name>A0A4W2EB60_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EB60"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044653.1">
        <property type="protein sequence ID" value="ENSBIXP00000033188.1"/>
        <property type="gene ID" value="ENSBIXG00000015925.1"/>
    </dbReference>
    <dbReference type="OMA" id="CYTESIL"/>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="359" mass="40885" checksum="316A345D0D770D64" modified="2019-09-18" version="1">MGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNIGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLLKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVADFVFLGSKITADGDCSQEIKRRLLLGRKVMTNLDSILKSRDITLPTKVSLVKAMAFPAVMYGCESWTVKKAQCQRIDAFELWCWRRLLRVLWTARRSNQSILKEISPGISLEGMILKLKLQYFGHLMRRVDSLEKTVMLGGIGGRRRRGRQRMRWLDGITDSMDMSLSELWELVMDREAWCAEIHGVAKSRTRLSNRSDLDLMTQQFHSWYISQKNTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBL8</accession>
    <name>A0A4W2EBL8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBL8"/>
    <dbReference type="STRING" id="30522.A0A4W2EBL8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047300.1">
        <property type="protein sequence ID" value="ENSBIXP00000036866.1"/>
        <property type="gene ID" value="ENSBIXG00000003734.1"/>
    </dbReference>
    <dbReference type="OMA" id="AFRNSWV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039208">
        <property type="entry name" value="C2_Ca-dependent_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47226">
        <property type="entry name" value="C2 CALCIUM-DEPENDENT DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47226:SF5">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="223"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="333" mass="35337" checksum="0AE28EE2CEF77CB9" modified="2019-09-18" version="1">RAKVGRLARRATALTPAGCPNVLTPDRIPEFCIPPRLVPCPTLTAVRDSWDEQAGTDDGAGHTDWDPRSQAALSLQHLPRARTAYGFCALLESPHTRRRESLFLGGTGAAALPPAPRPRAHTYGGGGGNDSLPPRARAPIAPPKARSGSLLDALGPGPRCRRFLRASEELLRRVLRAQRNRDLARGPSGSGSSARAPGASPSPTPSPLPERLEAEGTVTLGRAGGALRLAAEYSRASGRLRVRLLRAEGPAGGAAEPRAPVGCRVSFILKPRGAVVRRSRRAVLEQDLCLDGLSEDEVRRLAVRVKAENPGRGLQRGRLLGQGELLLGPLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEG8</accession>
    <name>A0A4W2EEG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EEG8"/>
    <dbReference type="STRING" id="30522.A0A4W2EEG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024255.1">
        <property type="protein sequence ID" value="ENSBIXP00000035335.1"/>
        <property type="gene ID" value="ENSBIXG00000018584.1"/>
    </dbReference>
    <dbReference type="OMA" id="YTHINCG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000155">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000189">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000740">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001070">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001353">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001393">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000566">
        <property type="entry name" value="zinc finger protein 133 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF122">
        <property type="entry name" value="SIMILAR TO ZINC FINGER PROTEIN 84 (HPF2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFN2</accession>
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        <recommendedName>
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            <taxon>Eutheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
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        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="F:signaling receptor binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ITPR-bd"/>
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    <dbReference type="InterPro" id="IPR029326">
        <property type="entry name" value="SSFA2_C"/>
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    <dbReference type="InterPro" id="IPR043444">
        <property type="entry name" value="TESPA1-like"/>
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    <dbReference type="PANTHER" id="PTHR17469:SF14">
        <property type="entry name" value="PROTEIN ITPRID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469">
        <property type="entry name" value="SPERM SPECIFIC ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14722">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF14723">
        <property type="entry name" value="SSFA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01257">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ITPR-interacting" evidence="4">
        <location>
            <begin position="111"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="314"/>
            <end position="403"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="483"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="592"/>
            <end position="617"/>
        </location>
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            <begin position="965"/>
            <end position="1046"/>
        </location>
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        <location>
            <begin position="904"/>
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            <begin position="7"/>
            <end position="24"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="356"/>
            <end position="365"/>
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        <location>
            <begin position="366"/>
            <end position="377"/>
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        <location>
            <begin position="386"/>
            <end position="398"/>
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            <begin position="604"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="991"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SMART" id="SM01257"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1046" mass="115274" checksum="791BE72B9D289029" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJF7</accession>
    <name>A0A4W2EJF7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="15">Sulfotransferase</fullName>
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    </protein>
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        <name evidence="17" type="primary">HS3ST5</name>
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    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 3-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:9831"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:70975"/>
            <dbReference type="EC" id="2.8.2.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="15"/>
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        <property type="nucleotide sequence ID" value="XM_027551168.1"/>
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        <property type="gene ID" value="ENSBIXG00000002291.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005009594.1">
        <property type="protein sequence ID" value="ENSBIXP00005003813.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158991"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0050819">
        <property type="term" value="P:negative regulation of coagulation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0046596">
        <property type="term" value="P:regulation of viral entry into host cell"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="NST/OST"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF46">
        <property type="entry name" value="HEPARAN SULFATE GLUCOSAMINE 3-O-SULFOTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="16">
        <location>
            <begin position="90"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="12">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="99"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="308"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00052516"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="345" mass="40366" checksum="64AE0C45F7E0CC7D" modified="2019-09-18" version="1">MLFKQQAWLRQKLLVLGSLAVGSLLYLVARVGSLDRLQPICPIEGRFGARGQAEFPLRALQFKRGLLHEFRKGNSSKEQVHLHDLVQQLPKAIIIGVRKGGTRALLEMLNLHPAVVKASQEIHFFDNDENYAKGIEWYRKKMPFSYPQQITIEKSPAYFITEEVPERIYKMNSSIKLLIIVREPTTRAISDYTQVLEGKERKNKTYYKFEKLAIDPNTCEVNTKYKAVRTSIYTKHLERWLKYFPIEQFHIVDGDRLITEPLPELQLVEKFLNLPPRISQYNLYFNATRGFYCLRFNIIFNKCLAGSKGRIHPEVDPSVITKLRKFFHPFNQKFYQITGRTLNWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKF4</accession>
    <name>A0A4W2EKF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC113876272</name>
        <name evidence="9" type="synonym">SULT2A1</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4-ethylphenol + 3'-phosphoadenylyl sulfate = 4-ethylphenyl sulfate + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49584"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:133681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:70608"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027371115.1">
        <property type="nucleotide sequence ID" value="XM_027515314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKF4"/>
    <dbReference type="SMR" id="A0A4W2EKF4"/>
    <dbReference type="STRING" id="30522.A0A4W2EKF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022892.1">
        <property type="protein sequence ID" value="ENSBIXP00000034747.1"/>
        <property type="gene ID" value="ENSBIXG00000007473.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037070.1">
        <property type="protein sequence ID" value="ENSBIXP00005042176.1"/>
        <property type="gene ID" value="ENSBIXG00005025492.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876272"/>
    <dbReference type="GeneTree" id="ENSGT00940000154432"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050656">
        <property type="term" value="F:3'-phosphoadenosine 5'-phosphosulfate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004027">
        <property type="term" value="F:alcohol sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004062">
        <property type="term" value="F:aryl sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047704">
        <property type="term" value="F:bile-salt sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050294">
        <property type="term" value="F:steroid sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050427">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006068">
        <property type="term" value="P:ethanol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042403">
        <property type="term" value="P:thyroid hormone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="34"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="286" mass="33927" checksum="887F2651D3107C1E" modified="2019-09-18" version="1">MTGKCVWFEGIPFPSLDYSPELLREVQESFLVKDKDVLLLTFPKSGTNWLIETVCLIYSKGDPKWVQSEPIWDRSPWVETKHGYELLKEKEGPRLISSHLPIQLFPKSFFKSKAKMIYLIRNPRDVLVSGYFFWRSAKFVKRPQSLEQYFEWFVEGNVVFGSWFDHARGWMSMRDKENFLILSYEEMKWDMRSTVEKICQFLGKKLEPEELNSVLKNSSFQAMKENSMSNYSLLKGQYFEENGQLLRKGVTGDWRNYFTVAQAETFDKLFQEKMADLPQDLFLWKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKV9</accession>
    <name>A0A4W2EKV9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spermatid nuclear transition protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TNP1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear transition protein 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379743.1">
        <property type="nucleotide sequence ID" value="XM_027523942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKV9"/>
    <dbReference type="STRING" id="30522.A0A4W2EKV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024755.1">
        <property type="protein sequence ID" value="ENSBIXP00000035552.1"/>
        <property type="gene ID" value="ENSBIXG00000018840.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035349.1">
        <property type="protein sequence ID" value="ENSBIXP00005021470.1"/>
        <property type="gene ID" value="ENSBIXG00005024456.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881115"/>
    <dbReference type="CTD" id="7141"/>
    <dbReference type="GeneTree" id="ENSGT00390000000539"/>
    <dbReference type="OMA" id="FKSHGMR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001673">
        <property type="term" value="C:male germ cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010954">
        <property type="term" value="P:positive regulation of protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035092">
        <property type="term" value="P:sperm DNA condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007290">
        <property type="term" value="P:spermatid nucleus elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001319">
        <property type="entry name" value="Nuclear_transition_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020062">
        <property type="entry name" value="Nuclear_transition_prot1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17486">
        <property type="entry name" value="SPERMATID NUCLEAR TRANSITION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17486:SF0">
        <property type="entry name" value="SPERMATID NUCLEAR TRANSITION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02079">
        <property type="entry name" value="TP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00541">
        <property type="entry name" value="TP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="55" mass="6456" checksum="7284082AD9133D79" modified="2019-09-18" version="1">MSTSRKLKSQGMRRGKNRTPHKGVKRSGSKRKYRKSSLKSRKRCDDANRNLRSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELL2</accession>
    <name>A0A4W2ELL2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Opioid growth factor receptor (OGFr) conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the opioid growth factor receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELL2"/>
    <dbReference type="STRING" id="30522.A0A4W2ELL2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027830.1">
        <property type="protein sequence ID" value="ENSBIXP00000036859.1"/>
        <property type="gene ID" value="ENSBIXG00000020369.1"/>
    </dbReference>
    <dbReference type="OMA" id="LHFAWEH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140625">
        <property type="term" value="F:opioid growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006757">
        <property type="entry name" value="OGF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039574">
        <property type="entry name" value="OGFr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015:SF1">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR OGFR ZETA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04664">
        <property type="entry name" value="OGFr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opioid growth factor receptor (OGFr) conserved" evidence="3">
        <location>
            <begin position="218"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="674" mass="74997" checksum="EB69CB50616A6D85" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPF2</accession>
    <name>A0A4W2EPF2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Galactocerebrosidase</fullName>
            <ecNumber evidence="3">3.2.1.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Galactocerebroside beta-galactosidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Galactosylceramidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Galactosylceramide beta-galactosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">GALC</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Hydrolyzes the galactose ester bonds of glycolipids such as galactosylceramide and galactosylsphingosine. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a D-galactosylceramide + H2O = an N-acyl-sphingoid base + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:43412"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:36498"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43413"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>beta-D-galactosyl-(1&lt;-&gt;1)-sphing-4-enine + H2O = sphing-4-enine + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:43908"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:57934"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:43909"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 59 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.46" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPF2"/>
    <dbReference type="STRING" id="30522.A0A4W2EPF2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032205.1">
        <property type="protein sequence ID" value="ENSBIXP00000038748.1"/>
        <property type="gene ID" value="ENSBIXG00000004407.1"/>
    </dbReference>
    <dbReference type="OMA" id="KYPKNGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004336">
        <property type="term" value="F:galactosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006683">
        <property type="term" value="P:galactosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.560:FF:000001">
        <property type="entry name" value="galactocerebrosidase precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000091">
        <property type="entry name" value="galactocerebrosidase precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000026">
        <property type="entry name" value="galactocerebrosidase precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049162">
        <property type="entry name" value="GH59_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049161">
        <property type="entry name" value="GH59_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001286">
        <property type="entry name" value="Glyco_hydro_59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035394">
        <property type="entry name" value="Glyco_hydro_59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15172">
        <property type="entry name" value="GALACTOCEREBROSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15172:SF1">
        <property type="entry name" value="GALACTOCEREBROSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02057">
        <property type="entry name" value="Glyco_hydro_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21708">
        <property type="entry name" value="Glyco_hydro_59_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17387">
        <property type="entry name" value="Glyco_hydro_59M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00850">
        <property type="entry name" value="GLHYDRLASE59"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0746">Sphingolipid metabolism</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 59 catalytic" evidence="21">
        <location>
            <begin position="60"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 59 central" evidence="22">
        <location>
            <begin position="362"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 59 C-terminal lectin" evidence="23">
        <location>
            <begin position="514"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="20">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="20">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601286-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF21708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="690" mass="77819" checksum="FB3A774298008E10" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ES62</accession>
    <name>A0A4W2ES62_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Keratocan</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ES62"/>
    <dbReference type="SMR" id="A0A4W2ES62"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034705.1">
        <property type="protein sequence ID" value="ENSBIXP00000039845.1"/>
        <property type="gene ID" value="ENSBIXG00000023856.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000092">
        <property type="entry name" value="keratocan isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000133">
        <property type="entry name" value="prolargin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF13">
        <property type="entry name" value="KERATOCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021220794" description="Keratocan" evidence="12">
        <location>
            <begin position="21"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="13">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="352" mass="40446" checksum="C736F06879088CE2" modified="2019-09-18" version="1">MASTICFILWVVFVTDTVWTRSVRQVYEASDPEDWTMHDFDCPRECFCPPSFPTALYCENRGLKEIPAIPSRIWYLYLENNLIETIPEKPFENATQLRWINLNKNKITNYGIEKGALSQLKKLLFLFLEDNELEEVPSPLPRSLEQLQLARNKVSRIPQGTFSNLENLTLLDLQHNKLLDNAFQRDTFKGLKNLMQLNMAKNALRNMPPRLPANTMQLFLDNNSIEGIPENYFNVIPKVAFLRLNHNKLSDAGLPSSGFNVSSILDLQLSHNQLTKVPKISAHLQHLYLDHNKIRNVNVSVICPSTPTTLPVEDSFSYGPHLRYLRLDGNEIKPPIPMDLMTCFRLLQAVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESD8</accession>
    <name>A0A4W2ESD8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginase-2, mitochondrial</fullName>
            <ecNumber evidence="4">3.5.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Arginase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Type II arginase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ARG2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESD8"/>
    <dbReference type="SMR" id="A0A4W2ESD8"/>
    <dbReference type="STRING" id="30522.A0A4W2ESD8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034793.1">
        <property type="protein sequence ID" value="ENSBIXP00000039883.1"/>
        <property type="gene ID" value="ENSBIXG00000023898.1"/>
    </dbReference>
    <dbReference type="OMA" id="YKEFRYA"/>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000008">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF4">
        <property type="entry name" value="ARGINASE-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0835">Urea cycle</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="462" mass="50181" checksum="09F7D71B6BE570FA" modified="2019-09-18" version="1">MEPMDSSLVLARHSRGVGLSPAPANSSVELASARRSFVRRESRSSSPRPRSWPCGVSGRGMSLARELGPEPSDTSESRGKEQQRAGPAGRRRSACSRLLSLLLPALPIMSLRSHLSRLLRTQVHSVRKKSVHSVAVIGAPFSQGQKRKGVEYGPAAVREAGLMKRLSDLGCHLKDFGDLNFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSGGYSCVTVGGDHSLAIGTISGHARHCPDLGVIWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSPSIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMEQTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGIYITEEIHSTGLLSALDLVEVNPRLAVSEEEAKATASLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESESEERVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUD2</accession>
    <name>A0A4W2EUD2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gluconokinase</fullName>
            <ecNumber evidence="8">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IDNK</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>D-gluconate + ATP = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="8"/>
    <dbReference type="RefSeq" id="XP_027405892.1">
        <property type="nucleotide sequence ID" value="XM_027550091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EUD2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036077.1">
        <property type="protein sequence ID" value="ENSBIXP00000040417.1"/>
        <property type="gene ID" value="ENSBIXG00000024430.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027909.1">
        <property type="protein sequence ID" value="ENSBIXP00005038128.1"/>
        <property type="gene ID" value="ENSBIXG00005020164.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897391"/>
    <dbReference type="CTD" id="414328"/>
    <dbReference type="GeneTree" id="ENSGT00390000003364"/>
    <dbReference type="OMA" id="YEGDDYH"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000522">
        <property type="entry name" value="Gluconokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442:SF3">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
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            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="185" mass="20671" checksum="746DF30EF4B6DFF5" modified="2019-09-18" version="1">MEAPNAVLVMGVSGSGKSTVGALLASELGWKFYDADDYHPEENRMKMQKGIPLNDEDRIPWLCKLHDVLRRDVASGQHVVLACSALKKVYRDILIQGKDDAPLKCDDSGKEEKPVEVKLLVVHLTGSFDIISGRLLRRKDHFMPPELLQSQFDTLETPSAPESFIQINVDKNLSEIIATIMETLK</sequence>
</entry>
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            <taxon>Eutheria</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="633" mass="70956" checksum="07FB7595FE6551BD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F051</accession>
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        <recommendedName>
            <fullName evidence="47">Amyloid-beta A4 protein</fullName>
        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="54 55" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="54" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="54" key="3">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="47">Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.</text>
    </comment>
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        <text evidence="1">The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.</text>
    </comment>
    <comment type="subcellular location">
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            <topology evidence="5 47">Single-pass type I membrane protein</topology>
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        <subcellularLocation>
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            <location evidence="12">Growth cone</location>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Early endosome</location>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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        <subcellularLocation>
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            <location evidence="10">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
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        <subcellularLocation>
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        <subcellularLocation>
            <location evidence="11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 46 47">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="46">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000042729.1"/>
        <property type="gene ID" value="ENSBIXG00000026721.1"/>
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        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005905">
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    <keyword evidence="44" id="KW-0966">Cell projection</keyword>
    <keyword evidence="41" id="KW-0168">Coated pit</keyword>
    <keyword evidence="48" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="36" id="KW-0186">Copper</keyword>
    <keyword evidence="15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="45" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="40 46" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="28" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="27" id="KW-0967">Endosome</keyword>
    <keyword evidence="42" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="37" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="21" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="35" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="39 47" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="33" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="43" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="55" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0964">Secreted</keyword>
    <keyword evidence="32" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="26 50" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0765">Sulfation</keyword>
    <keyword evidence="23 47" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="34 47" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="30" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="29" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="50">
        <location>
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        </location>
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    <feature type="chain" id="PRO_5021287220" description="Amyloid-beta A4 protein" evidence="50">
        <location>
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            <end position="752"/>
        </location>
    </feature>
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        <location>
            <begin position="683"/>
            <end position="705"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
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    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="51">
        <location>
            <begin position="291"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="53">
        <location>
            <begin position="374"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="46">
        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="46">
        <location>
            <begin position="131"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="49">
        <location>
            <begin position="194"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="48">
        <location>
            <begin position="399"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="49">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="49">
        <location>
            <begin position="228"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="49">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="46">
        <location>
            <begin position="73"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="46">
        <location>
            <begin position="98"/>
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    </feature>
    <feature type="disulfide bond" evidence="46">
        <location>
            <begin position="133"/>
            <end position="187"/>
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    </feature>
    <feature type="disulfide bond" evidence="46">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="46">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00009449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
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                <person name="Tearle R."/>
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</entry>
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                <person name="Bickhart D.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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            <taxon>Artiodactyla</taxon>
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    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="7">Acts as a transcriptional activator and repressor required for cardiac development and may have key roles in the maintenance of functional and structural phenotypes in adult heart.</text>
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            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
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    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395314.1">
        <property type="nucleotide sequence ID" value="XM_027539513.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2FNR5"/>
    <dbReference type="STRING" id="30522.A0A4W2FNR5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046195.1">
        <property type="protein sequence ID" value="ENSBIXP00000013716.1"/>
        <property type="gene ID" value="ENSBIXG00000018599.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004731.1">
        <property type="protein sequence ID" value="ENSBIXP00005006256.1"/>
        <property type="gene ID" value="ENSBIXG00005011799.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891457"/>
    <dbReference type="CTD" id="57057"/>
    <dbReference type="GeneTree" id="ENSGT00940000158741"/>
    <dbReference type="OMA" id="EDGHTTH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003180">
        <property type="term" value="P:aortic valve morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0060413">
        <property type="term" value="P:atrial septum morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:atrioventricular canal development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008015">
        <property type="term" value="P:blood circulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001569">
        <property type="term" value="P:branching involved in blood vessel morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0003207">
        <property type="term" value="P:cardiac chamber formation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0055008">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:outflow tract septum morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of epithelial to mesenchymal transition"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Transcription factor, T-box"/>
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        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
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        <property type="entry name" value="TF_T-box"/>
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        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11267:SF190">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX20"/>
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        <property type="entry name" value="T-box"/>
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        <property type="entry name" value="TBOX"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TBOX_2"/>
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        <property type="entry name" value="TBOX_3"/>
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    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="106"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="62"/>
            <end position="83"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="73"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    </evidence>
    <sequence length="449" mass="49467" checksum="B534D6168305BEDD" modified="2019-09-18" version="1">MEFTASPKPQLSSRANAFSIAALMSSGGSKEKEATENTIKPLEQFVEKSSCTQPLGELTSLDAHGEFGGSGAGSSSPSSSSLCTEPLIPTTPIIPSEEMAKIACSLETKELWDKFHELGTEMIITKSGRRMFPTIRVSFSGVDSEAKYIVLMDIVPVDNKRYRYAYHRSSWLVAGKADPPLPARLYVHPDSPFTGEQLLKQMVSFEKVKLTNNELDQHGHIILNSMHKYQPRVHIIKKKDHTASLLNLKSEEFRTFIFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRDSSRLTDIERESVESLIQKHSYARSPIRTYGGEEDVLGDEVQTTQNRGSAFTTSDNLSLSSWVSSSSSFPAFQHPQSLTALGTSTASIATPIPHPIQGSLPPYSRLGMPLTPSAIASSMQGSGPTFPSFHMPRYHHYFQQGPYAAIQGLRHSSAVMTPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FQH9</accession>
    <name>A0A4W2FQH9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane protein 59 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM59L</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM59 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027404123.1">
        <property type="nucleotide sequence ID" value="XM_027548322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FQH9"/>
    <dbReference type="STRING" id="30522.A0A4W2FQH9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038790.1">
        <property type="protein sequence ID" value="ENSBIXP00000023281.1"/>
        <property type="gene ID" value="ENSBIXG00000025775.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001285.1">
        <property type="protein sequence ID" value="ENSBIXP00005007966.1"/>
        <property type="gene ID" value="ENSBIXG00005013127.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896173"/>
    <dbReference type="CTD" id="25789"/>
    <dbReference type="GeneTree" id="ENSGT00390000008279"/>
    <dbReference type="OMA" id="DNAHKVN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001658">
        <property type="term" value="P:branching involved in ureteric bud morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022065">
        <property type="entry name" value="Uncharacterised_TMEM59"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28652:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 59-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28652">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 59-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12280">
        <property type="entry name" value="BSMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611430" evidence="10">
        <location>
            <begin position="23"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="349" mass="38990" checksum="54E31C30E2689A37" modified="2019-09-18" version="1">MDSVALMPLLLLLLLQPPPATPAPPVRDPFALQLGDTQNCQLRCRDRYPKPQLAQEELGEDPIQSRRAKAYDRAVLTSACERGCRLFSICRFVARSSKPNATQAECEAACVEAYVKETEQQACSEGCWSQNPEPEPEPELEQKRKVLEAPSGALSLLDLFSTLCNDLVNSAQGFVSSTWTYYLQTDNGKVVVFQTQPVVESLGHEGARLQRVEVTWRGSHPEALEVHVDPVGPLDKVRKAKIRVKTSSKAKVESDELQDNDFLSCMSRRSGLPRWILACCLFLSVLVMLWLSCSTLVTAPGQHLKFQPLTLEQHKGYMVEPEWSLYPPPSHAYADSPPPYKLKLDLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G1Q8</accession>
    <name>A0A4W2G1Q8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA guanine-7 methyltransferase activating subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RAMAC</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376541.1">
        <property type="nucleotide sequence ID" value="XM_027520740.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027376542.1">
        <property type="nucleotide sequence ID" value="XM_027520741.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027376543.1">
        <property type="nucleotide sequence ID" value="XM_027520742.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027376544.1">
        <property type="nucleotide sequence ID" value="XM_027520743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G1Q8"/>
    <dbReference type="SMR" id="A0A4W2G1Q8"/>
    <dbReference type="STRING" id="30522.A0A4W2G1Q8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000656.1">
        <property type="protein sequence ID" value="ENSBIXP00000008008.1"/>
        <property type="gene ID" value="ENSBIXG00000013860.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046089.1">
        <property type="protein sequence ID" value="ENSBIXP00005012297.1"/>
        <property type="gene ID" value="ENSBIXG00005002665.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879407"/>
    <dbReference type="CTD" id="83640"/>
    <dbReference type="GeneTree" id="ENSGT00390000011190"/>
    <dbReference type="OMA" id="PPIIEEW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106005">
        <property type="term" value="P:RNA 5'-cap (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028271">
        <property type="entry name" value="RAMAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48168">
        <property type="entry name" value="RNA GUANINE-7 METHYLTRANSFERASE-ACTIVATING SUBUNIT-LIKE (PSEUDOGENE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48168:SF1">
        <property type="entry name" value="RNA GUANINE-N7 METHYLTRANSFERASE ACTIVATING SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15320">
        <property type="entry name" value="RAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="118" mass="14280" checksum="3A90871E6D07746B" modified="2019-09-18" version="1">MTDTSEAVPNFEEMFASRFTEDDKEYQEYLKRPPDSPPIVEEWNSRAGGSLRNRGNRLQDNRQFRGRDSRRGWPSDNRSNQWHGRSWGNNYPQHRQEPYYPHQYGHYGYNQRPPYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GC74</accession>
    <name>A0A4W2GC74_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA 3'-terminal phosphate cyclase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RCL1</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">As part of the small subunit (SSU) processome, it plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Activates BMS1 by promoting GDP/GTP exchange. Does not have cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with BMS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405146.1">
        <property type="nucleotide sequence ID" value="XM_027549345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GC74"/>
    <dbReference type="SMR" id="A0A4W2GC74"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047722.1">
        <property type="protein sequence ID" value="ENSBIXP00000037444.1"/>
        <property type="gene ID" value="ENSBIXG00000003957.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026519.1">
        <property type="protein sequence ID" value="ENSBIXP00005015589.1"/>
        <property type="gene ID" value="ENSBIXG00005019414.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897063"/>
    <dbReference type="CTD" id="10171"/>
    <dbReference type="GeneTree" id="ENSGT00530000063404"/>
    <dbReference type="OMA" id="YTDQNKG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000001">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.20:FF:000001">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="9">
        <location>
            <begin position="184"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005015589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="373" mass="41010" checksum="303BCDC0956576E8" modified="2019-09-18" version="1">MASQAHSLSYSGCNFLRQRLVLSTLSGRPVKIRKIRARDDNPGLRDFEASFIRLLDKVTNGSRIEINQTGTTLYYQPGLLYGGSMEHDCSVLRGIGYYLESLLCLAPFMKHPLRIVLRGVTNDQVDPSVDVLKATALPLLKQFGIDGESFELKVVRRGMPPGGGGEVFFSCPVRKVLKPIQLIDPGKIKRIRGMAYSVRVSPQMANRIVDSARSILNKFIPDIYIYTDHMKGINSGKSPGFGLSLVAETTNGTFLSAELASNPQGQGAAMLPEDLGRNCARLLLEEIYRGGCVDSTNQSLALLLMTLGQQDVSKVLLGPLSPYTIEFLRHLKSFFQIMFKIETKPCGEELKGGDKVLMTCVGIGFSNLSKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GE28</accession>
    <name>A0A4W2GE28_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mesoderm posterior bHLH transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MESP2</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GE28"/>
    <dbReference type="STRING" id="30522.A0A4W2GE28"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016179.1">
        <property type="protein sequence ID" value="ENSBIXP00000008455.1"/>
        <property type="gene ID" value="ENSBIXG00000014294.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048120.1">
        <property type="protein sequence ID" value="ENSBIXP00005016256.1"/>
        <property type="gene ID" value="ENSBIXG00005019976.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063712"/>
    <dbReference type="OMA" id="GWAGHSD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032525">
        <property type="term" value="P:somite rostral/caudal axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18938">
        <property type="entry name" value="bHLH_TS_Mesp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000047">
        <property type="entry name" value="mesoderm posterior protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040259">
        <property type="entry name" value="Mesogenin/MesP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937:SF17">
        <property type="entry name" value="MESODERM POSTERIOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="80"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="366" mass="39057" checksum="AC8CE4AB295CF316" modified="2019-09-18" version="1">MAQSPPLQSLLGQDHWIFPQGWGWVGHSDSTSPASSSSDSSGSCPCDRARRPSQPAPAARGAAEAASTAPSRARTRPAGGQRQSASEREKLRMRTLARALHELRRFLPPSVAPAGQSLTKIETLRLAIRYIGHLSAVLGLSEDSLQRRRLQRSDAAPPRGCALCPDGGPAEAQRQGLLLRSAAGAAVSCRSPPVCPEALAAPERLGSRVPDMGPWLTPPYCPGMQSPPQLSQGRAPDAALWTSPQACCGMQTPPEPRNQPTPWTSPPASLELAAVYQGISVSPESCLLPETPPLLPRPACQRLQPQTQWGCWSHTAEVLPNSEDQGPGPALQLSDESPPQNSGLPLSGCPEFWQEDLVETHLGMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GTM1</accession>
    <name>A0A4W2GTM1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Low molecular weight phosphotyrosine protein phosphatase</fullName>
            <shortName evidence="14">LMW-PTP</shortName>
            <shortName evidence="14">LMW-PTPase</shortName>
            <ecNumber evidence="3 14">3.1.3.2</ecNumber>
            <ecNumber evidence="4 14">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 14">Low molecular weight cytosolic acid phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ACP1</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15018"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1. Interacts with the SH3 domain of SPTAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3 14"/>
    <dbReference type="EC" id="3.1.3.48" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_027406555.1">
        <property type="nucleotide sequence ID" value="XM_027550754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GTM1"/>
    <dbReference type="SMR" id="A0A4W2GTM1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033524.1">
        <property type="protein sequence ID" value="ENSBIXP00000039319.1"/>
        <property type="gene ID" value="ENSBIXG00000004587.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034101.1">
        <property type="protein sequence ID" value="ENSBIXP00005020665.1"/>
        <property type="gene ID" value="ENSBIXG00005004078.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897766"/>
    <dbReference type="CTD" id="52"/>
    <dbReference type="GeneTree" id="ENSGT00940000162690"/>
    <dbReference type="OMA" id="ICHEDYD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16343">
        <property type="entry name" value="LMWPTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000105">
        <property type="entry name" value="Low molecular weight phosphotyrosine protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002115">
        <property type="entry name" value="Tyr_Pase_low_mol_wt_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF34">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00720">
        <property type="entry name" value="MAMMALPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="15">
        <location>
            <begin position="7"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="158" mass="18169" checksum="5895A7947E4B5D38" modified="2019-09-18" version="1">MAEQVTKSVLFVCLGNICRSPIAEAVFRKLVTDQNISDNWRIDSAATSTYELGNPPDFRGQACMRKHGIPMSHVARQVTKEDFVTFDYILCMDESNLRDLNRKSNQVKNCRAKIELLGSYDPQKQLIIEDPYYGNDADFETVYQQCVRCCRAFLEKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H1B4</accession>
    <name>A0A4W2H1B4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/arginine-rich splicing factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Splicing factor, arginine/serine-rich 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SRSF1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Consists of two polypeptides of p32 and p33. Identified in the spliceosome C complex. Component of a ribonucleoprotein complex containing mRNAs and RNA-binding proteins including DDX5, HNRNPH2 and SRSF1 as well as splicing regulator ARVCF. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus). Interacts with SRPK1 and a sliding docking interaction is essential for its sequential and processive phosphorylation by SRPK1. Interacts with NXF1. Interacts with CCNL1, CCNL2 and CDK11B. Interacts with RRP1B. Interacts (when phosphorylated in its RS domain) with TNPO3; promoting nuclear import. Interacts with ILDR1 (via C-terminus) and ILDR2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372378.1">
        <property type="nucleotide sequence ID" value="XM_027516577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H1B4"/>
    <dbReference type="SMR" id="A0A4W2H1B4"/>
    <dbReference type="STRING" id="30522.A0A4W2H1B4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010159.1">
        <property type="protein sequence ID" value="ENSBIXP00000003281.1"/>
        <property type="gene ID" value="ENSBIXG00000009702.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052421.1">
        <property type="protein sequence ID" value="ENSBIXP00005024450.1"/>
        <property type="gene ID" value="ENSBIXG00005004643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876974"/>
    <dbReference type="CTD" id="6426"/>
    <dbReference type="GeneTree" id="ENSGT00940000155585"/>
    <dbReference type="OMA" id="PREPAYP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044547">
        <property type="term" value="F:DNA topoisomerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050733">
        <property type="term" value="F:RS domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097400">
        <property type="term" value="P:interleukin-17-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110012">
        <property type="term" value="P:protein localization to P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023019">
        <property type="term" value="P:signal transduction involved in regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12597">
        <property type="entry name" value="RRM1_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12767">
        <property type="entry name" value="RRM2_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000053">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000170">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034520">
        <property type="entry name" value="SRSF1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029538">
        <property type="entry name" value="SRSF1_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF66">
        <property type="entry name" value="SERINE_ARGININE-RICH SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="121"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="191"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="205"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="248" mass="27745" checksum="C28A0B2F112EA713" modified="2019-09-18" version="1">MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HAI6</accession>
    <name>A0A4W2HAI6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small integral membrane protein 45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SMIM45</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HAI6"/>
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        <property type="protein sequence ID" value="ENSBIXP00000005843.1"/>
        <property type="gene ID" value="ENSBIXG00000011972.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015591.1">
        <property type="protein sequence ID" value="ENSBIXP00005027896.1"/>
        <property type="gene ID" value="ENSBIXG00005029896.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:neuron maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="68" mass="8048" checksum="90DC220333A7F21F" modified="2019-09-18" version="1">MPHFLDWFVPVYLFISVLILVGFGACIYYFEPGLQEAHKWRMQRPLVDRDFRKTLMVRDNLAFGGPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HQ47</accession>
    <name>A0A4W2HQ47_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PFDN1</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402091.1">
        <property type="nucleotide sequence ID" value="XM_027546290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HQ47"/>
    <dbReference type="SMR" id="A0A4W2HQ47"/>
    <dbReference type="STRING" id="30522.A0A4W2HQ47"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051251.1">
        <property type="protein sequence ID" value="ENSBIXP00000042134.1"/>
        <property type="gene ID" value="ENSBIXG00000026242.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016861.1">
        <property type="protein sequence ID" value="ENSBIXP00005033299.1"/>
        <property type="gene ID" value="ENSBIXG00005014050.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895283"/>
    <dbReference type="CTD" id="5201"/>
    <dbReference type="GeneTree" id="ENSGT00390000009786"/>
    <dbReference type="OMA" id="REMIQQK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905907">
        <property type="term" value="P:negative regulation of amyloid fibril formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23164">
        <property type="entry name" value="Prefoldin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000006">
        <property type="entry name" value="prefoldin subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="122" mass="14223" checksum="C7183F8917FD8820" modified="2019-09-18" version="1">MAAPVDLELKKAFTELQAKVIDTQQKVKLADVQIEQLNRTKKHAHLTDTEIMTLVDETNMYEGVGRMFILQSKEAIHNQLLEKQKIAEEKIKELEQKKSYLERSVKEAEDNIREMLMARRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HWU9</accession>
    <name>A0A4W2HWU9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2HWU9"/>
    <dbReference type="STRING" id="30522.A0A4W2HWU9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044180.1">
        <property type="protein sequence ID" value="ENSBIXP00000009534.1"/>
        <property type="gene ID" value="ENSBIXG00000002158.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026701.1">
        <property type="protein sequence ID" value="ENSBIXP00005037604.1"/>
        <property type="gene ID" value="ENSBIXG00005019479.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF21">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE ISOZYME 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="101" mass="11508" checksum="CB0C7ED6AA5ECCD4" modified="2019-09-18" version="1">MFLQKELPVRLANTMREVNLLLEDLLNNPSVELVQSWYILPFSLHVFNYSETELELTQAAPSAGFGCGLPISRLYARYFQGDLKLYSMEGVVTDAVIYPFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HXC2</accession>
    <name>A0A4W2HXC2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketimine reductase mu-crystallin</fullName>
            <ecNumber evidence="21">1.5.1.1</ecNumber>
            <ecNumber evidence="3">1.5.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">NADP-regulated thyroid-hormone-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CRYM</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3R)-1,4-thiomorpholine-3-carboxylate + NAD(+) = 3,4-dehydrothiomorpholine-3-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58517"/>
            <dbReference type="ChEBI" id="CHEBI:176873"/>
            <dbReference type="EC" id="1.5.1.25"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:12506"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3R)-1,4-thiomorpholine-3-carboxylate + NADP(+) = 3,4-dehydrothiomorpholine-3-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58517"/>
            <dbReference type="ChEBI" id="CHEBI:176873"/>
            <dbReference type="EC" id="1.5.1.25"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:12502"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-lanthionine ketimine + NADPH + 2 H(+) = (3R,5R)-1,4-thiomorpholine-3,5-dicarboxylate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68040"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:176891"/>
            <dbReference type="ChEBI" id="CHEBI:176892"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:68041"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-cystathionine ketimine + NADH + 2 H(+) = (3R,5S)-2,3,5,6,7-pentahydro-1,4-thiazepine-3,5-dicarboxylate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:68032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:176808"/>
            <dbReference type="ChEBI" id="CHEBI:176810"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:68033"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(S)-cystathionine ketimine + NADPH + 2 H(+) = (3R,5S)-2,3,5,6,7-pentahydro-1,4-thiazepine-3,5-dicarboxylate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68036"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:176808"/>
            <dbReference type="ChEBI" id="CHEBI:176810"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:68037"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Delta(2)-thiazoline-2-carboxylate + NADPH + 2 H(+) = L-thiazolidine-2-carboxylate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68072"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:176895"/>
            <dbReference type="ChEBI" id="CHEBI:176896"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:68073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-pipecolate + NAD(+) = Delta(1)-piperideine-2-carboxylate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30807"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:61185"/>
            <dbReference type="ChEBI" id="CHEBI:77631"/>
            <dbReference type="EC" id="1.5.1.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:30809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-pipecolate + NADP(+) = Delta(1)-piperideine-2-carboxylate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12524"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61185"/>
            <dbReference type="ChEBI" id="CHEBI:77631"/>
            <dbReference type="EC" id="1.5.1.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:12526"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-proline + NAD(+) = 1-pyrroline-2-carboxylate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:39785"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:20323"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-proline + NADP(+) = 1-pyrroline-2-carboxylate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:39785"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:20319"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homodimer. Binds the thyroid hormone triiodothyronine (T3); T3 binding inhibits enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.1" evidence="21"/>
    <dbReference type="EC" id="1.5.1.25" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027383245.1">
        <property type="nucleotide sequence ID" value="XM_027527444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HXC2"/>
    <dbReference type="SMR" id="A0A4W2HXC2"/>
    <dbReference type="STRING" id="30522.A0A4W2HXC2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010024.1">
        <property type="protein sequence ID" value="ENSBIXP00000003348.1"/>
        <property type="gene ID" value="ENSBIXG00000009760.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023532.1">
        <property type="protein sequence ID" value="ENSBIXP00005036230.1"/>
        <property type="gene ID" value="ENSBIXG00005017725.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883584"/>
    <dbReference type="CTD" id="1428"/>
    <dbReference type="GeneTree" id="ENSGT00390000000237"/>
    <dbReference type="OMA" id="VKIVNVH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047125">
        <property type="term" value="F:delta1-piperideine-2-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050241">
        <property type="term" value="F:pyrroline-2-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047127">
        <property type="term" value="F:thiomorpholine-carboxylate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070324">
        <property type="term" value="F:thyroid hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042403">
        <property type="term" value="P:thyroid hormone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070327">
        <property type="term" value="P:thyroid hormone transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1780.10:FF:000001">
        <property type="entry name" value="Ketimine reductase mu-crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000241">
        <property type="entry name" value="ketimine reductase mu-crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00093273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00093567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005036230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="314" mass="33747" checksum="D9EAE1F47B7CAE2B" modified="2019-09-18" version="1">MSSRPVFLSAADVQDHLRSSSLLIAPLETALANFSSGPDGGVVQPVRTVVPVAKHRGFLGVMPAYSAAEDALTTKLVTFYEDHSATSTVPSHQATVLLFQPSNGSLLAVMDGNVITAKRTAAVSAIATKFLKPPNSEVLCILGAGVQAYSHYEVFTEQFFFKEVRIWNRTKENAEKFVNTVPGEVRICSSVQEAVTGADVIITVTMATEPILFGEWVKPGAHINAIGASRPDWRELDDELMKQAVLYVDSQEAALKESGDVLLSGAEIFAELGEVVKGVKPAHCEKTTVFKSLGMAVEDMVAAKLVYDSWSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I117</accession>
    <name>A0A4W2I117_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Potassium voltage-gated channel subfamily Q member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCNQ2</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027414935.1">
        <property type="nucleotide sequence ID" value="XM_027559134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I117"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027463.1">
        <property type="protein sequence ID" value="ENSBIXP00000015832.1"/>
        <property type="gene ID" value="ENSBIXG00000003687.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026180.1">
        <property type="protein sequence ID" value="ENSBIXP00005037370.1"/>
        <property type="gene ID" value="ENSBIXG00005019244.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903269"/>
    <dbReference type="CTD" id="3785"/>
    <dbReference type="GeneTree" id="ENSGT00940000160093"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020969">
        <property type="entry name" value="Ankyrin-G_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003947">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF4">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16642">
        <property type="entry name" value="KCNQ2_u3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11956">
        <property type="entry name" value="KCNQC3-Ank-G_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01461">
        <property type="entry name" value="KCNQ2CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="96"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="22">
        <location>
            <begin position="466"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="448"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="697"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="839"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="673"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="873" mass="96002" checksum="F56EA5874BC7C0D7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IBB2</accession>
    <name>A0A4W2IBB2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase</fullName>
            <ecNumber evidence="17">1.1.1.145</ecNumber>
            <ecNumber evidence="16">5.3.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC113888503</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid</text>
            <dbReference type="Rhea" id="RHEA:14709"/>
            <dbReference type="ChEBI" id="CHEBI:47907"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="EC" id="5.3.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 3beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24076"/>
            <dbReference type="ChEBI" id="CHEBI:1722"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47907"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; steroid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="21">Steroid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.145" evidence="17"/>
    <dbReference type="EC" id="5.3.3.1" evidence="16"/>
    <dbReference type="RefSeq" id="XP_027391408.1">
        <property type="nucleotide sequence ID" value="XM_027535607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IBB2"/>
    <dbReference type="SMR" id="A0A4W2IBB2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035578.1">
        <property type="protein sequence ID" value="ENSBIXP00000021099.1"/>
        <property type="gene ID" value="ENSBIXG00000024269.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030999.1">
        <property type="protein sequence ID" value="ENSBIXP00005039522.1"/>
        <property type="gene ID" value="ENSBIXG00005021936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113888503"/>
    <dbReference type="GeneTree" id="ENSGT00940000155444"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003854">
        <property type="term" value="F:3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004769">
        <property type="term" value="F:steroid Delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000220">
        <property type="entry name" value="3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="23">
        <location>
            <begin position="7"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00057122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00060577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="373" mass="42220" checksum="B9BDB817E7B5A1D1" modified="2019-09-18" version="1">MAGWSCLVTGGGGFLGQRIICLLVEEKDLQEIRVLDKVFRPEVREEFSKLQSKIKLTLLEGDILDEQCLKGACQGTSVVIHTASVIDVRNAVPRETIMNVNVKGTQLLLEACVQASVPVFIHTSTIEVAGPNSYREIIQDGREEEHHESAWSSPYPYSKKLAEKAVLGANGWALKNGGTLYTCALRPMYIYGEGSPFLSAYMHGALNNNGILTNHCKFSRVNPVYVGNVAWAHILALRALRDPKKVPNIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSRMSLPISLQYWLAFLLEIVSFLLSPIYKYNPCFNRHLVTLSNSVFTFSYKKAQRDLGYEPLYTWEEAKQKTKEWIGSLVKQHKETLKTKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IJ79</accession>
    <name>A0A4W2IJ79_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">OR2L13</name>
        <name evidence="14" type="synonym">LOC113896285</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2IJ79"/>
    <dbReference type="STRING" id="30522.A0A4W2IJ79"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036523.1">
        <property type="protein sequence ID" value="ENSBIXP00000021725.1"/>
        <property type="gene ID" value="ENSBIXG00000005030.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052546.1">
        <property type="protein sequence ID" value="ENSBIXP00005043510.1"/>
        <property type="gene ID" value="ENSBIXG00005004682.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286923"/>
    <dbReference type="OMA" id="TVPNMAF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15421">
        <property type="entry name" value="7tmA_OR2T-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="311" mass="35667" checksum="90838973F8DFA8F6" modified="2019-09-18" version="1">MEKWNQTSNDFILLGLFLPNQTGWLLLLLIILIFFLASVGNSAMIYLICLDPRLHTPMYFLLSQLSLMDLMYLSTTVPKMVFNFLSGQKGISYLGCGVQSFFFLTMACSEGLLLASMAYDRYVAICHPLYYCICMSKRMCMKMIMGSWILGSLNSLAHTVYALHIPYCQSRAIDHFFCDVPAMLPLACMDTWVYEYMVFVSTSLFLLLPFLGITASYGRVLFAVYHMRSKDGRKKAFTTCSTHLTVVTFYYAPFVYTYLRPRNLRSPAEDKILAVFYTILTPMLNPIIYSLRNKEVLGAMTRMFGKFSFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IK42</accession>
    <name>A0A4W2IK42_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endophilin-B1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">SH3 domain-containing GRB2-like protein B1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SH3GLB1</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027392463.1">
        <property type="nucleotide sequence ID" value="XM_027536662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IK42"/>
    <dbReference type="SMR" id="A0A4W2IK42"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031034.1">
        <property type="protein sequence ID" value="ENSBIXP00000018167.1"/>
        <property type="gene ID" value="ENSBIXG00000022040.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043917.1">
        <property type="protein sequence ID" value="ENSBIXP00005045625.1"/>
        <property type="gene ID" value="ENSBIXG00005029220.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889666"/>
    <dbReference type="CTD" id="51100"/>
    <dbReference type="GeneTree" id="ENSGT00940000155667"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07616">
        <property type="entry name" value="BAR_Endophilin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11945">
        <property type="entry name" value="SH3_Endophilin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000017">
        <property type="entry name" value="endophilin-B2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000028">
        <property type="entry name" value="endophilin-B2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035695">
        <property type="entry name" value="SH3GLB1_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028503">
        <property type="entry name" value="SH3GLB_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF52">
        <property type="entry name" value="ENDOPHILIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="19">
        <location>
            <begin position="27"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="305"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="365" mass="40777" checksum="0613B91EA16E5C16" modified="2019-09-18" version="1">MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRASSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYHSNNNQTAVAPVPSASSNVIGSSALTSTSSLVITSPSNLTDLKECGGSRRARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGRVPITYLELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BKZ5</accession>
    <name>A0A4W2BKZ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amelogenin X-linked</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amelogenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BKZ5"/>
    <dbReference type="STRING" id="30522.A0A4W2BKZ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053020.1">
        <property type="protein sequence ID" value="ENSBIXP00000000506.1"/>
        <property type="gene ID" value="ENSBIXG00000029755.1"/>
    </dbReference>
    <dbReference type="OMA" id="NMLRYPY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030345">
        <property type="term" value="F:structural constituent of tooth enamel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070166">
        <property type="term" value="P:enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004116">
        <property type="entry name" value="Amelogenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46794:SF2">
        <property type="entry name" value="AMELOGENIN, X ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46794">
        <property type="entry name" value="AMELOGENIN, Y ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02948">
        <property type="entry name" value="Amelogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01757">
        <property type="entry name" value="AMELOGENIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00818">
        <property type="entry name" value="Amelogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0091">Biomineralization</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021261537" description="Amelogenin X-linked" evidence="7">
        <location>
            <begin position="17"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22009" checksum="A49E29ACD9B6A2B4" modified="2019-09-18" version="1">MGTWILFACLLGGAYSMPLPPHPGHPGYINFSYEVLTPLKWYQNMLRYPYPSYGYEPVGGWLHHQIIPVVSQQSPQNHALQPHHHNPMVPAQQPVVPQQPMMPVPGQHSMTPIQHHQPNLPLPAQQSFQPQPIQPQPHQPLQPQPPVHPIQRLPPQPPLPPIFPMQPLPPVLPDLPLEAWPATDKTKREEVVSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMT8</accession>
    <name>A0A4W2BMT8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMT8"/>
    <dbReference type="STRING" id="30522.A0A4W2BMT8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053360.1">
        <property type="protein sequence ID" value="ENSBIXP00000000324.1"/>
        <property type="gene ID" value="ENSBIXG00000029958.1"/>
    </dbReference>
    <dbReference type="OMA" id="GRRICFR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="307" mass="34261" checksum="16A7EE64F3184D3A" modified="2019-09-18" version="1">RRGANQSSVSEFLLLGLSRQPQQQQLLFLLFLTMYLATVLGNLLILLAISVDSRLHIPMYFFLCNLSFVDICFSSTTVPKVLANHILGRQTISFSGCLMQMYFLFEFADMDNFLLAVMAYDRFVAVCHPLHYSAKMTPQLCALLVTGSWVTASLNALLHTLLMARLSFCADNAIPHFFCDVTPLLKLSCSDTHLSEVMILTEGALIMITPFVCILASYVRITCAVLRIPSTKGRWKAFSTCGSHLAMVSLFYGTIIAVYFNPLSSHSSEKDTVATVMYTVVTSMLNPFIYSLRNRDLKRALGKVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BN47</accession>
    <name>A0A4W2BN47_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">NAD(+) hydrolase SARM1</fullName>
            <ecNumber evidence="6">3.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">NADP(+) hydrolase SARM1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Sterile alpha and TIR motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>NAD(+) = cyclic ADP-beta-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:73672"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:38612"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>NADP(+) + H2O = ADP-D-ribose 2'-phosphate + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19849"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58673"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:19850"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SARM1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BN47"/>
    <dbReference type="SMR" id="A0A4W2BN47"/>
    <dbReference type="STRING" id="30522.A0A4W2BN47"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014156.1">
        <property type="protein sequence ID" value="ENSBIXP00000001386.1"/>
        <property type="gene ID" value="ENSBIXG00000028634.1"/>
    </dbReference>
    <dbReference type="OMA" id="KSCEVQT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="28" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5021369536" description="NAD(+) hydrolase SARM1" evidence="28">
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            <begin position="16"/>
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        <location>
            <begin position="486"/>
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        <location>
            <begin position="560"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="704"/>
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    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048388"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="728" mass="80227" checksum="84B2E16046EB64CC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTV0</accession>
    <name>A0A4W2BTV0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="7">Ankyrin repeat and SOCS box protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ASB12</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with CUL5 and RNF7.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000008860.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat and SOCS box containing 12"/>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052391">
        <property type="entry name" value="E3_Ligase-Neurotoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24133">
        <property type="entry name" value="ANKYRIN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24133:SF33">
        <property type="entry name" value="ANKYRIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="317" mass="34896" checksum="A96A6FE5D84D626B" modified="2019-09-18" version="1">MRIVLLQSAKVNLMDITKIFSLLQPDDEEDTNTGEKQALNQAVYNNDSYTLDQLLCQERYKRFINSRSGWGVPGTPLRLAAAYGHLSCLQVLLAHGADVDSLDVKAQTPLFTAVSHGHLDCVRVLLEAGACPGGSIYNNCSPVLTAARDGAVTILQELLGHGAEVNVKAKLPVWASNITSCSGPLYLAAVYGHLDCFRLLLLHGADPDYNCTDQRLLARVSRPRTLLEICLHHNCEPEYIQLLIDFGANIYLPSLCLDLNSQYDGTALLVQARATPRSLLSQARLVIRRSLCHTSQPQAIDQLDIPPMLISYLKHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BU07</accession>
    <name>A0A4W2BU07_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dynactin subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DCTN5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BU07"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011206.1">
        <property type="protein sequence ID" value="ENSBIXP00000002776.1"/>
        <property type="gene ID" value="ENSBIXG00000009239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027786.1">
        <property type="protein sequence ID" value="ENSBIXP00005016439.1"/>
        <property type="gene ID" value="ENSBIXG00005007063.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015360"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03359">
        <property type="entry name" value="LbH_Dynactin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000014">
        <property type="entry name" value="dynactin subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047125">
        <property type="entry name" value="DCTN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126:SF1">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21711">
        <property type="entry name" value="DCTN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="152" mass="16633" checksum="3CC638F012D63D11" modified="2019-09-18" version="1">MELGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVVPPFTIFSGCPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYK4</accession>
    <name>A0A4W2BYK4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Dynein axonemal assembly factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 10">Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell projection</location>
            <location evidence="1 10">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the DNAAF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYK4"/>
    <dbReference type="STRING" id="30522.A0A4W2BYK4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007852.1">
        <property type="protein sequence ID" value="ENSBIXP00000004424.1"/>
        <property type="gene ID" value="ENSBIXG00000010767.1"/>
    </dbReference>
    <dbReference type="OMA" id="SWRVETE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000349">
        <property type="entry name" value="Dynein assembly factor 1, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000394">
        <property type="entry name" value="Dynein assembly factor 1, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF19">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 10" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 10" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="49"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="303"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="616"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="821"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="651"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="831"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="927" mass="101214" checksum="CDD79C3EA518C419" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZQ6</accession>
    <name>A0A4W2BZQ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">X-ray repair cross-complementing protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="33">5'-deoxyribose-5-phosphate lyase Ku70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">ATP-dependent DNA helicase 2 subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">ATP-dependent DNA helicase II 70 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="34">CTC box-binding factor 75 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">DNA repair protein XRCC6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37" type="primary">XRCC6</name>
    </gene>
    <organism evidence="37 38">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="37 38 39" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ku70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396454.1">
        <property type="nucleotide sequence ID" value="XM_027540653.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027396455.1">
        <property type="nucleotide sequence ID" value="XM_027540654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZQ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004777.1">
        <property type="protein sequence ID" value="ENSBIXP00000005928.1"/>
        <property type="gene ID" value="ENSBIXG00000012063.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000256.1">
        <property type="protein sequence ID" value="ENSBIXP00005027757.1"/>
        <property type="gene ID" value="ENSBIXG00005030124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892168"/>
    <dbReference type="CTD" id="2547"/>
    <dbReference type="GeneTree" id="ENSGT00940000153239"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002684">
        <property type="term" value="P:positive regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01458">
        <property type="entry name" value="vWA_ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000007">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000010">
        <property type="entry name" value="X-ray repair cross-complementing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000031">
        <property type="entry name" value="X-ray repair cross-complementing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.970.10:FF:000001">
        <property type="entry name" value="X-ray repair cross-complementing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000001">
        <property type="entry name" value="X-ray repair cross-complementing protein 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.970.10">
        <property type="entry name" value="Ku70, bridge and pillars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027388">
        <property type="entry name" value="Ku70_bridge/pillars_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00578">
        <property type="entry name" value="ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="22" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="24" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="25" id="KW-0234">DNA repair</keyword>
    <keyword evidence="21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-0347">Helicase</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0391">Immunity</keyword>
    <keyword evidence="10" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="26" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="28" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SAP" evidence="36">
        <location>
            <begin position="570"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="35">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="35">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00071962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00079373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00080458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00083456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00083699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00083706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="607" mass="69943" checksum="030C3C15D1E6717B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7Z1</accession>
    <name>A0A4W2C7Z1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the membrane-bound acyltransferase family. HHAT subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7Z1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001306.1">
        <property type="protein sequence ID" value="ENSBIXP00000007691.1"/>
        <property type="gene ID" value="ENSBIXG00000013622.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF20">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE HHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="493" mass="57046" checksum="6882C773C28EB36D" modified="2019-09-18" version="1">MLPRWELSLYLLASIGFHFYSFYEVYKVSREHEEELDREFDLETGTLFRGLKKDPTDFEWSFWMEWGKERLVWLFLGHMSVSQVANLISRKHKPWILAVYGMWACRCVLGTQGTAMVLIHATISFCVAQFRSQFLSWLCSLSLLSTLRLQDVEEVKRGWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQPPPAERPFYSLPWMLAYVFYYPVFHNGPVLSFPEFIAQMQQQEQSSPKTSLSILALGLGRLLFYWWLAELMVHLMYMHAIYSSASLLRAVSSWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLRPPPLPRCVSTMFSFTGMWRHFDVGLHDFLVRYVYIPAGGSQHGLLGALFSTAMTFAFVSFWHGGNDYLWCWATLNWLGVTVENGVQKLVQRPHVQHSLAQFLSPGARRRLHAALASCSTSMLILSNLVFLGGSQVGEIYWDRIFIQGWPWVTLSVLGFLYCYSHVGIAWAHTYSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCI3</accession>
    <name>A0A4W2CCI3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCI3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008671.1">
        <property type="protein sequence ID" value="ENSBIXP00000004039.1"/>
        <property type="gene ID" value="ENSBIXG00000010398.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016747.1">
        <property type="protein sequence ID" value="ENSBIXP00005033249.1"/>
        <property type="gene ID" value="ENSBIXG00005013973.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="OMA" id="FRAGMEY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="32"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="415" mass="47747" checksum="C58CF7358FF68E07" modified="2019-09-18" version="1">MNKASGGYRIPVELFQILKDDAVKVLHSICQKIWKTQQWPQDWKRSVFIPIPKKGNVKECSNYRTIALISHASKVMFKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANIHWIIEKAREFQKNIYFCFTDYAKAFDYVDQNKLWEILKEMGIPDHLTCLLRNLYAGQEAIVRTGHGKTHWFQIGKGVRKGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTILMAENEKELKSLLVKVKEESEKVGLKQNIQKTKIMASGPITSWQIDGETVSDFIFGGSKITADGDCSHEIKRCILLGRKVITNLDSILKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKNAECRRIDAFELWCWRRLLRVPWTAKRSNQSILKEISPGCSLEGLMLKLKLQYFGYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CD18</accession>
    <name>A0A4W2CD18_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin-16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Kidney-specific cadherin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CDH16</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CD18"/>
    <dbReference type="SMR" id="A0A4W2CD18"/>
    <dbReference type="STRING" id="30522.A0A4W2CD18"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018323.1">
        <property type="protein sequence ID" value="ENSBIXP00000009828.1"/>
        <property type="gene ID" value="ENSBIXG00000015449.1"/>
    </dbReference>
    <dbReference type="OMA" id="INLPTVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000167">
        <property type="entry name" value="Cadherin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000187">
        <property type="entry name" value="Cadherin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000195">
        <property type="entry name" value="Cadherin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000240">
        <property type="entry name" value="Cadherin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000241">
        <property type="entry name" value="Cadherin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000149">
        <property type="entry name" value="cadherin-16 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000159">
        <property type="entry name" value="cadherin-16 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF424">
        <property type="entry name" value="CADHERIN-16 ISOFORM X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021447374" description="Cadherin-16" evidence="18">
        <location>
            <begin position="19"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="56"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="127"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="264"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="337"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="451"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="565"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="671"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="829" mass="90039" checksum="EE3B774B33D2E31B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CD20</accession>
    <name>A0A4W2CD20_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CD20"/>
    <dbReference type="STRING" id="30522.A0A4W2CD20"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020573.1">
        <property type="protein sequence ID" value="ENSBIXP00000010826.1"/>
        <property type="gene ID" value="ENSBIXG00000006893.1"/>
    </dbReference>
    <dbReference type="OMA" id="RETRNIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050657">
        <property type="entry name" value="Ankyrin_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24147">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24147:SF62">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="274" mass="30405" checksum="AA0C84F20B6D4CDE" modified="2019-09-18" version="1">MVEFLVKKEADIHAVDKMKRTALILAVNYECTDVVGLLLQRGADVFSPDVFGRTAEEYAAISGFNIICELISEYKEKRPKTTPENSNPAAKSSEEDSLSRFSNKPGVDSWPTSDDDMLGSETKKEPKPNLAKLMKAFQQSKRNQAECGIVRRENTTLSENNNSDSEIEDVAETLPKPSPGVQGFSHPASPRPDPLPKPLKTLAGLGLAKVEEEKKHKDAADESRLIQQRKSGGNNKQAFPAMENKGSHRFLIVGEKQEVCCMKTTCRMQLPWQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFY0</accession>
    <name>A0A4W2CFY0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CFY0"/>
    <dbReference type="STRING" id="30522.A0A4W2CFY0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021608.1">
        <property type="protein sequence ID" value="ENSBIXP00000011902.1"/>
        <property type="gene ID" value="ENSBIXG00000002214.1"/>
    </dbReference>
    <dbReference type="OMA" id="NGMLAVH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000663">
        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000070">
        <property type="entry name" value="zinc finger protein 689 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000229">
        <property type="entry name" value="Zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF371">
        <property type="entry name" value="ZINC FINGER PROTEIN 112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="523"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="579"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="607"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="636" mass="71680" checksum="998342F1316B9CCF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHJ9</accession>
    <name>A0A4W2CHJ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Colipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CLPS</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHJ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005095.1">
        <property type="protein sequence ID" value="ENSBIXP00000005773.1"/>
        <property type="gene ID" value="ENSBIXG00000011907.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001981">
        <property type="entry name" value="Colipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017914">
        <property type="entry name" value="Colipase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017915">
        <property type="entry name" value="Colipase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02740">
        <property type="entry name" value="Colipase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00128">
        <property type="entry name" value="COLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00023">
        <property type="entry name" value="COLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57190">
        <property type="entry name" value="Colipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00121">
        <property type="entry name" value="COLIPASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51342">
        <property type="entry name" value="COLIPASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021500426" evidence="1">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Colipase C-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="70" mass="7548" checksum="219FDC4F8A4014A7" modified="2019-09-18" version="1">MEKVLILLLVALAVAYAVPDPGIIINLTLYGIYYKCPCERGLTCNVDKTIVGSITNTNFGVCLDLGRATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHU5</accession>
    <name>A0A4W2CHU5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHU5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022716.1">
        <property type="protein sequence ID" value="ENSBIXP00000012658.1"/>
        <property type="gene ID" value="ENSBIXG00000003013.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032396">
        <property type="term" value="F:inhibitory MHC class I receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002764">
        <property type="term" value="P:immune response-regulating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000049">
        <property type="entry name" value="Leukocyte immunoglobulin-like receptor subfamily B member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050412">
        <property type="entry name" value="Ig-like_Receptors_ImmuneReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11738:SF172">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11738">
        <property type="entry name" value="MHC CLASS I NK CELL RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021410295" description="Immunoglobulin domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="7">
        <location>
            <begin position="34"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="7">
        <location>
            <begin position="129"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="341" mass="37026" checksum="2553C543AA623976" modified="2019-09-18" version="1">MSMVPPSLLCLGLCLSQSTWAQKGNLPPPRITALPGSTVPAKSHVTLLCQGPKQAEGYRISRVGSPEPMDKEEQITSRKTNALSIAEMTSGKTGLYHCSYQRGGRWSQFSDPLQLVMTGAYDKPSLSSMAGTVVSPGEKVKLQCVSKINHDVFILTKEDGDHVTQNQSSVPHDGGRQTIFLLNPVSSTQAGTFRCYGAFRDNPYVWSQPSDPLQLQVEGTTDSPSSTQPRCSTAPSGNRASQDIRLGIWIGVPVAVGLLLLVFLIFLILYCLRKAKNNAERKERHPEAAGWVNGQASEAADPQDVTYSQVTCHVPHQGTAGTPSWVPRQTQSSEYVTLAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLF4</accession>
    <name>A0A4W2CLF4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cyclin L2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CCNL2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family. Cyclin L subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLF4"/>
    <dbReference type="STRING" id="30522.A0A4W2CLF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046268.1">
        <property type="protein sequence ID" value="ENSBIXP00000013935.1"/>
        <property type="gene ID" value="ENSBIXG00000006992.1"/>
    </dbReference>
    <dbReference type="OMA" id="NYEEVML"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20590">
        <property type="entry name" value="CYCLIN_CCNL2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20593">
        <property type="entry name" value="CYCLIN_CCNL2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000014">
        <property type="entry name" value="cyclin-L1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000016">
        <property type="entry name" value="cyclin-L1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21797">
        <property type="entry name" value="CycT2-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CR17</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="144" mass="16260" checksum="392F775A1238FBF4" modified="2019-09-18" version="1">MPAKTPELEMMDEDRLIEEVLDKFVNCHEQTYEEFLRTFTHLSKDVLLLPGQVEQEVSLSVPSYIPSVAIQPVTPGTKPRPTVKAADKQSKEIVGDEVQPFSLDEEFDYDAVVLTPKFTPAEMNAIKELSKQKTKSADLEDPHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CS57</accession>
    <name>A0A4W2CS57_BOBOX</name>
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        <submittedName>
            <fullName evidence="13">Solute carrier family 25 member 51</fullName>
        </submittedName>
    </protein>
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        <name evidence="13" type="primary">SLC25A51</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405497.1">
        <property type="nucleotide sequence ID" value="XM_027549696.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405498.1">
        <property type="nucleotide sequence ID" value="XM_027549697.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405499.1">
        <property type="nucleotide sequence ID" value="XM_027549698.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405500.1">
        <property type="nucleotide sequence ID" value="XM_027549699.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405501.1">
        <property type="nucleotide sequence ID" value="XM_027549700.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405502.1">
        <property type="nucleotide sequence ID" value="XM_027549701.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405503.1">
        <property type="nucleotide sequence ID" value="XM_027549702.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405504.1">
        <property type="nucleotide sequence ID" value="XM_027549703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CS57"/>
    <dbReference type="SMR" id="A0A4W2CS57"/>
    <dbReference type="STRING" id="30522.A0A4W2CS57"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025696.1">
        <property type="protein sequence ID" value="ENSBIXP00000035972.1"/>
        <property type="gene ID" value="ENSBIXG00000019325.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000046645.1">
        <property type="protein sequence ID" value="ENSBIXP00000014693.1"/>
        <property type="gene ID" value="ENSBIXG00000019325.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014111.1">
        <property type="protein sequence ID" value="ENSBIXP00005001503.1"/>
        <property type="gene ID" value="ENSBIXG00005008001.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014118.1">
        <property type="protein sequence ID" value="ENSBIXP00005001500.1"/>
        <property type="gene ID" value="ENSBIXG00005008001.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897213"/>
    <dbReference type="CTD" id="92014"/>
    <dbReference type="GeneTree" id="ENSGT00940000155622"/>
    <dbReference type="OMA" id="GCAFNTG"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990549">
        <property type="term" value="P:mitochondrial NAD transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046495">
        <property type="term" value="P:nicotinamide riboside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052465">
        <property type="entry name" value="Mito_NAD+_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131:SF2">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER SLC25A51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131">
        <property type="entry name" value="SD08549P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="28"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="213"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="297" mass="33957" checksum="9B43B4421446C59C" modified="2019-09-18" version="1">MMDSEAHEKRPPILTSSKQDIAPHIATVSEMKHYLCGCCAAFNNIAITFPIQKVLFRQQLYGIKTRDAILQLRRDGFRNLYRGILPPLMQKTTTLALMFGLYEDLSCLLRKHVSTPEFATRSMAAVLAGTTEAIFTPLERVQTLLQDHRHHDKFTNTYQAFKALKCHGIREYYRGLVPVLFRNGFSNVLFFGLRGPIKEHLPTATTHSAHLVNDFICGGLLGAMLGILFFPVNVVKTRMQSQIGGEFQSFPKVFQKIWLERDRKLTNLFRGAHLNYHRSLISWGIINATYEFLLKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CT62</accession>
    <name>A0A4W2CT62_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CT62"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000046801.1">
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        <property type="gene ID" value="ENSBIXG00000003506.1"/>
    </dbReference>
    <dbReference type="OMA" id="NEWRINC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="497"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="872" mass="101512" checksum="7DA57E9AF4A6B10E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTN2</accession>
    <name>A0A4W2CTN2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTN2"/>
    <dbReference type="STRING" id="30522.A0A4W2CTN2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026665.1">
        <property type="protein sequence ID" value="ENSBIXP00000015291.1"/>
        <property type="gene ID" value="ENSBIXG00000019837.1"/>
    </dbReference>
    <dbReference type="OMA" id="PKYILFQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20180">
        <property type="entry name" value="Dpy19L4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dpy-19/Dpy-19-like"/>
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    <dbReference type="InterPro" id="IPR047464">
        <property type="entry name" value="Dpy19L4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488:SF2">
        <property type="entry name" value="C-MANNOSYLTRANSFERASE DPY19L4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488">
        <property type="entry name" value="DPY-19-LIKE 1, LIKE (H. SAPIENS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10034">
        <property type="entry name" value="Dpy19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="178"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="202"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="263"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="337"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="370"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="441"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="466"/>
            <end position="483"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="511"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="523"/>
            <end position="542"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008744"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="723" mass="83722" checksum="5B5B64F0D929E0FC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVC4</accession>
    <name>A0A4W2CVC4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cadherin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Epithelial cadherin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">CDH1</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.</text>
    </comment>
    <comment type="function">
        <text evidence="23">E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 27">Cell membrane</location>
            <topology evidence="2 27">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CVC4"/>
    <dbReference type="STRING" id="30522.A0A4W2CVC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018990.1">
        <property type="protein sequence ID" value="ENSBIXP00000010250.1"/>
        <property type="gene ID" value="ENSBIXG00000002394.1"/>
    </dbReference>
    <dbReference type="OMA" id="GAVNNCM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00031">
        <property type="entry name" value="CA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000191">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000027">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014868">
        <property type="entry name" value="Cadherin_pro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF319">
        <property type="entry name" value="CADHERIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08758">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01055">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15 26 27" id="KW-0106">Calcium</keyword>
    <keyword evidence="16 27" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="17" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 30" id="KW-0732">Signal</keyword>
    <keyword evidence="10 27 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 29" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021241247" description="Cadherin-1" evidence="30">
        <location>
            <begin position="23"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="710"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="31">
        <location>
            <begin position="187"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="31">
        <location>
            <begin position="263"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="31">
        <location>
            <begin position="376"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="31">
        <location>
            <begin position="487"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="748"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="28">
        <location>
            <begin position="756"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00025086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00032684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00059145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="883" mass="97975" checksum="01B3115CF617DC6C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWZ7</accession>
    <name>A0A4W2CWZ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADP-sugar pyrophosphatase</fullName>
            <ecNumber evidence="9">2.7.7.96</ecNumber>
            <ecNumber evidence="2">3.6.1.13</ecNumber>
            <ecNumber evidence="8">3.6.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">8-oxo-dGDP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Nuclear ATP-synthesis protein NUDIX5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nucleoside diphosphate-linked moiety X motif 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>8-oxo-dGDP + H2O = 8-oxo-dGMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32063"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63224"/>
            <dbReference type="ChEBI" id="CHEBI:63715"/>
            <dbReference type="EC" id="3.6.1.58"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ADP-D-ribose + H2O = D-ribose 5-phosphate + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:10412"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-ribose 5-phosphate + ATP + H(+) = ADP-D-ribose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:50248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="2.7.7.96"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Interacts with PARG.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.96" evidence="9"/>
    <dbReference type="EC" id="3.6.1.13" evidence="2"/>
    <dbReference type="EC" id="3.6.1.58" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWZ7"/>
    <dbReference type="STRING" id="30522.A0A4W2CWZ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019930.1">
        <property type="protein sequence ID" value="ENSBIXP00000010856.1"/>
        <property type="gene ID" value="ENSBIXG00000007636.1"/>
    </dbReference>
    <dbReference type="OMA" id="NDPGLCN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000016">
        <property type="entry name" value="ADP-sugar pyrophosphatase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="15">
        <location>
            <begin position="56"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00084011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="218" mass="24179" checksum="B7A63534E1D02D45" modified="2019-09-18" version="1">MEQETAGSSRNTEQSIISEELIAEGKWVKLEKTTYRDPTGKTRTWETVKRTTRKGQSADGVAIIPVLQRTLHYECIILVKQFRPPMGGYCLEFPAGLIDDNESPEAAALRELEEETGYKGDVAECSPAVCMDPGLSNCTAHIVTVTINGDDAENVRPKPKPGDGEFVEVISLPKNDLLNRLEALIAEEHVMVDARVYSYALALKHANTKPFEVPFLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CX77</accession>
    <name>A0A4W2CX77_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA transcription, translation and transport factor protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RTRAF</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RTRAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027409554.1">
        <property type="nucleotide sequence ID" value="XM_027553753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CX77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047494.1">
        <property type="protein sequence ID" value="ENSBIXP00000016444.1"/>
        <property type="gene ID" value="ENSBIXG00000020627.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050069.1">
        <property type="protein sequence ID" value="ENSBIXP00005019956.1"/>
        <property type="gene ID" value="ENSBIXG00005023121.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900211"/>
    <dbReference type="CTD" id="51637"/>
    <dbReference type="GeneTree" id="ENSGT00390000005163"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019265">
        <property type="entry name" value="RTRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15924">
        <property type="entry name" value="CLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10036">
        <property type="entry name" value="RLL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="245" mass="28209" checksum="563062C8BD38A203" modified="2019-09-18" version="1">MFRRKLTALDYHNPAGFNCKDETEFRNFIVWLEDQKIRHYKIEDRGNLRNIHSSDWPKFFEKYLRDVNCPFKIQDRQEAIDWLLGLAVRLEYGDNAEKYNKDLVPDNTKNADNAAKNAEPLINLDVNNPDFKAGVMALANLLQIQRHDDYLVMLKAIRILVQERLTQDAVAKANQMKEGLPVALDKHILGFDTGDAVLNEAAQILRLLHIEELRELQTKINEAIVAVQAIIADPKTDHRLGKVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CX89</accession>
    <name>A0A4W2CX89_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Limbic system associated membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LSAMP</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CX89"/>
    <dbReference type="STRING" id="30522.A0A4W2CX89"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030815.1">
        <property type="protein sequence ID" value="ENSBIXP00000018032.1"/>
        <property type="gene ID" value="ENSBIXG00000004233.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001368.1">
        <property type="protein sequence ID" value="ENSBIXP00005032554.1"/>
        <property type="gene ID" value="ENSBIXG00005006335.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158516"/>
    <dbReference type="OMA" id="ITHVMEP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF22">
        <property type="entry name" value="LIMBIC SYSTEM-ASSOCIATED MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="116"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="203"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="345" mass="38024" checksum="F628024C2213E6A4" modified="2025-10-08" version="2">MRLLCLLPTGLPVRSVDFNRGTDNITVRQGDTAILRCVVEDKNSKVAWLNRSGIIFAGHDKWSLDPRVELEKRHSLEYSLRIQKVDVYDEGSYTCSVQTQHEPKTSQVYLIVQVPPKISNISSDVTVNEGSNVTLVCMANGRPEPVITWRHLTPTGREFEGEEEYLEILGITREQSGKYECKAANEVSSADVKQVKVTVNYPPTITESKSNEATTGRKASLKCEASAVPAPDFEWYRDDTRINSANGLEIKSTEGQSSLTVTNVTEEHYGNYTCVAANNLGMTNASLVLFKRVSPTIPHPLQEIGTTVHFKQKGPGSVRGINGSISLAVPLWLLAASLLCLLSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1J8</accession>
    <name>A0A4W2D1J8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Phytanoyl-CoA dioxygenase, peroxisomal</fullName>
            <ecNumber evidence="10">1.14.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phytanic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Phytanoyl-CoA alpha-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PHYH</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-methylbutanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylbutanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54612"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:138296"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:54613"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-methyldodecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methyldodecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55192"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138636"/>
            <dbReference type="ChEBI" id="CHEBI:138637"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:55193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3-methylhexadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylhexadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:44000"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58784"/>
            <dbReference type="ChEBI" id="CHEBI:83969"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:44001"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3-methylnonanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylnonanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55180"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138633"/>
            <dbReference type="ChEBI" id="CHEBI:138634"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:55181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>3-methylundecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylundecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55184"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:84183"/>
            <dbReference type="ChEBI" id="CHEBI:138632"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:55185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>butanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxybutanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55176"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:138628"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:55177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>decanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxydecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54604"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
            <dbReference type="ChEBI" id="CHEBI:138292"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:54605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>dodecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxydodecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54628"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:138299"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:54629"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>eicosanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyeicosanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54620"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:138298"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54621"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>heptadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyheptadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54616"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74307"/>
            <dbReference type="ChEBI" id="CHEBI:138297"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:54617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hexadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyhexadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54596"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:54597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyhexanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55172"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
            <dbReference type="ChEBI" id="CHEBI:138630"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:55173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>octadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyoctadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54624"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:74116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54625"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>octanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyoctanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54600"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:138290"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54601"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>phytanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyphytanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16065"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57334"/>
            <dbReference type="ChEBI" id="CHEBI:57391"/>
            <dbReference type="EC" id="1.14.11.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tetradecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxytetradecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54632"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:138300"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:54633"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="29">
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PhyH family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.18" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1J8"/>
    <dbReference type="STRING" id="30522.A0A4W2D1J8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022486.1">
        <property type="protein sequence ID" value="ENSBIXP00000012524.1"/>
        <property type="gene ID" value="ENSBIXG00000017585.1"/>
    </dbReference>
    <dbReference type="OMA" id="SCHYANY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048244">
        <property type="term" value="F:phytanoyl-CoA dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000012">
        <property type="entry name" value="Phytanoyl-CoA dioxygenase, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047128">
        <property type="entry name" value="PhyH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308">
        <property type="entry name" value="PHYTANOYL-COA ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308:SF1">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0847">Vitamin C</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00053028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00071213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="337" mass="38797" checksum="776F61DD6EABEC87" modified="2019-09-18" version="1">MDRNRASARLTVLLRHLGCRSAGTIIAHHTSGVGSLASFHPQQFQYTRENNVLSLEQRKFYEENGFLVIKNLVSDADIQRFRNEFERICRKEVKPLGLSVMRDVTITKSEYVPSEKVVSKVQDFQEDEELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSNNIVCAWTAMEHIDRNNGCLVVLPGTHKGPLQPHDYPQWEGGVNIMFHGIQDYDKNNARVHLVMEKGDTVFFHPLLIHGSGRNKSQGFRKAISCHFADANCHYIDVEGTSQENIEKEVVDIVRKKYGFKDVTLKDVWTFRGRVVKGERINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1N3</accession>
    <name>A0A4W2D1N3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113899431</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1N3"/>
    <dbReference type="STRING" id="30522.A0A4W2D1N3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030913.1">
        <property type="protein sequence ID" value="ENSBIXP00000018082.1"/>
        <property type="gene ID" value="ENSBIXG00000006720.1"/>
    </dbReference>
    <dbReference type="OMA" id="TDPVNMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15913">
        <property type="entry name" value="7tmA_OR11G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF201">
        <property type="entry name" value="OLFACTORY RECEPTOR 11H1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="57"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="327" mass="36816" checksum="C70C95666ADBFD48" modified="2019-09-18" version="1">CLLMLQVTDPVNMNISEPDSSFVFVREFILLGFSYEWKIQSLLFSLFLTIYALTITGNGAIICALWCDQRLHIPMYIFLGNFSFLEIWYVSSTVPKMLVNFLSHKKTISFVGCFLQFYFFSSLGATECLHLTVMAFDRYLAICRPLHYPNIMTGHLCTKLVLICWVCGFLWFLVPIVLISQMPFCGPNIIDHVVCDPGPLFALACVSAPKTQLLCYSLSSIVIFGNFLFILGSYTLVLTAVLHMPSATGRQKAFSTCGSHLAVVSLFYGSLMVMYVSPGLGHSARMQKVTTLFYAMVTPFFNPLIYSLRNKEIKTALRKVLGSFNIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1T2</accession>
    <name>A0A4W2D1T2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear receptor-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NRBP1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Homodimer. Binds to MLF1, recruiting a serine kinase which phosphorylates both itself and MLF1. Phosphorylated MLF1 binds to YWHAZ and is retained in the cytoplasm. Interacts with ELOC/TCEB1, ELOB/TCEB2, TSC22D2 and TSC22D4. Interacts with the Elongin BC E3 ubiquitin ligase complex via its interaction with ELOB/TCEB2 and ELOC/TCEB1. Interacts with SALL4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1T2"/>
    <dbReference type="STRING" id="30522.A0A4W2D1T2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031046.1">
        <property type="protein sequence ID" value="ENSBIXP00000018155.1"/>
        <property type="gene ID" value="ENSBIXG00000004266.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000237.1">
        <property type="protein sequence ID" value="ENSBIXP00005000506.1"/>
        <property type="gene ID" value="ENSBIXG00005030132.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155605"/>
    <dbReference type="OMA" id="NEVQYAQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14034">
        <property type="entry name" value="PK_NRBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000181">
        <property type="entry name" value="Nuclear receptor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000098">
        <property type="entry name" value="Nuclear receptor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="68"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="409"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="484" mass="54068" checksum="85B43F453857C010" modified="2019-09-18" version="1">MSEGEPQTVLSSGSDPKVESSSSTPGLTSVSPPVTSTTSAASPEEEEESEDESEILEESPCGRWQKRREEVNQRNVPGIDSAYLAMDTEEGVEVVWNEVQFSERKNYKLQEEKVRAVFDNLIQLEHLNIVKFHKYWADIKENKARVIFITEYMSSGSLKQFLKKTKKNHKTMNEKAWKRWCTQILSALSYLHSCDPPIIHGNLTCDTIFIQHNGLIKIGSVAPDTINNHVKTCREEQKNLHFFAPEYGEVTNVTTAVDIYSFGMCALEMAVLEIQGNGESSYVPQEAISSAIQLLEDPLQREFIQKCLQSEPARRPTARELLFHPALFEVPSLKLLAAHCIVGHQHMIPENALEEITKNMDTSAVLAEIPAGPGREPVQTLYSQSPALELDKFLEDVRNGIYPLTAFGLPRPQQPQQEEVTSPVVPPSVKTPTPEPAEVETRKVVLMQCNIESVEEGVKHHVRLRAQGCVALGGQRVGKGSLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D330</accession>
    <name>A0A4W2D330_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Interleukin-3</fullName>
            <shortName evidence="15">IL-3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 15">Hematopoietic growth factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 15">Mast cell growth factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 15">Multipotential colony-stimulating factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">P-cell-stimulating factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113896625</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the IL-3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027404648.1">
        <property type="nucleotide sequence ID" value="XM_027548847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D330"/>
    <dbReference type="SMR" id="A0A4W2D330"/>
    <dbReference type="STRING" id="30522.A0A4W2D330"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034218.1">
        <property type="protein sequence ID" value="ENSBIXP00000020240.1"/>
        <property type="gene ID" value="ENSBIXG00000023590.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024838.1">
        <property type="protein sequence ID" value="ENSBIXP00005014477.1"/>
        <property type="gene ID" value="ENSBIXG00005018475.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896625"/>
    <dbReference type="GeneTree" id="ENSGT00940000163393"/>
    <dbReference type="OMA" id="IKDGDWN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005135">
        <property type="term" value="F:interleukin-3 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000067">
        <property type="entry name" value="Interleukin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002183">
        <property type="entry name" value="IL-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48489">
        <property type="entry name" value="INTERLEUKIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48489:SF1">
        <property type="entry name" value="INTERLEUKIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02059">
        <property type="entry name" value="IL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001939">
        <property type="entry name" value="IL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00430">
        <property type="entry name" value="INTERLEUKIN3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0202">Cytokine</keyword>
    <keyword evidence="10 15" id="KW-0339">Growth factor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610687" description="Interleukin-3" evidence="16">
        <location>
            <begin position="24"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="144" mass="16380" checksum="57CD0E826BCD0F26" modified="2019-09-18" version="1">MSSLSILHLLLLLLALHAPQAKGLPVVTSRTPYSMLMKEIMDDLKKITPSPEGSLNSDEKNFLTKESLLQANLKVFMTFATDTFGSDSKIMKNLKEFQPVLPTATPTEDPIFIENKNLGDFRMKLEEYLVIIRNYLKSKNIWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D429</accession>
    <name>A0A4W2D429_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="198"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="427" mass="49431" checksum="2B5CA8AF29ACC4F2" modified="2019-09-18" version="1">MEVHDIVQETGIKTIPMEKKCKKAKWLSVEALQIAIKRREAKSKGEKERYKHLNAEFQRIARRDKKAFFSDQCKEIEENNKMGKTRNLFKKIRDTKGTFHAKMSSIKDRNCMDLTEAEDIKKRWQEYTEELYKKDLHNPDNHDGVITDLEPDILECEVKWALESISTNKASGGDGIPVELFQILKDDAVQVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANIRWIIEKAREFQKNTYFCFIDYAKAFDCVDHNQLWKILKEMGIPGHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSRLMKVKVESEKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4A1</accession>
    <name>A0A4W2D4A1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">5-hydroxytryptamine receptor 3C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Postsynaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4A1"/>
    <dbReference type="STRING" id="30522.A0A4W2D4A1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034907.1">
        <property type="protein sequence ID" value="ENSBIXP00000020683.1"/>
        <property type="gene ID" value="ENSBIXG00000023956.1"/>
    </dbReference>
    <dbReference type="OMA" id="MRVVSIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19063">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000042">
        <property type="entry name" value="5-hydroxytryptamine receptor 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000068">
        <property type="entry name" value="LOW QUALITY PROTEIN: 5-hydroxytryptamine receptor 3C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049944">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022269592" description="5-hydroxytryptamine receptor 3C" evidence="18">
        <location>
            <begin position="26"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="52"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="253"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="51299" checksum="AEC848C16B111914" modified="2019-09-18" version="1">MEQGWLHGEGFLLCLILHLLLQGRGDTFTINCSGFDQHGVDPAAFQAAFARKAFRPATNVSIPIRVNISFTLSAILEVDAQLQLMTSFLWLNMIWYNPFITWNPEECGGIRKLGMATENLWLPDIFIEELMDTDQTPPGLMAYINSNGHIKYSKPMRVTSICNLDIFYFPFDEQNCTLTFSSFVYTVEKMVLGMEQDVQEILETSQNIIWNKGEWVLQGIHQMMKMTAGTNGYDQIVFYVAIRRRPTLYVINLLVPSSFLVAIDALSFYLPAESENRASFKMTLLLGYNVFLLMMNDLLPSSSTSLISVYFALCLSLMVLSLLETIFITYLLHLATTQPPPMPRWLHSLLLHCTSPRTCCPAVPWKGNMDLAHLPGVKEPMELVGKVPHPRETELNGCPGSARNQQEDKAQKLHLVHLWVRLSHMMDTLLFRLYLLFMATSVVTVIILWNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8E2</accession>
    <name>A0A4W2D8E2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RGP1 homolog, RAB6A GEF complex partner 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">RGP1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8E2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026215.1">
        <property type="protein sequence ID" value="ENSBIXP00000015015.1"/>
        <property type="gene ID" value="ENSBIXG00000019601.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="388" mass="42086" checksum="2E6F34D848DE88C6" modified="2019-09-18" version="1">MIEVVAELSRGPVFLAGEALECVVTVTNPLPPTATSASSEALAWASAQIHCQFHASESRVALPPPDSSQPDVQPESQTVLLPHRGERGQCILSTPPKILFCDLRLDPGESKSYSYSEVLPVEGPPSFRGQSVKYVYKLTIGCQRVNSPITLLRVPLRVLVLTGLQDVRFPQDEAVAPSSPFLEEDEGGKKDSWLTELAGERLMAATSCRSLHLYNISDGRGKVGTFGIFKSVYRLGEDVVGTLNLGEGTVACLQFSVSLQTEERVQPEYQRRRGAGGAPSVSHITHARHQESCLHTTRTSFSLPIPLSSTPGFCTAIVSLKWRLHFEFVTSREPGLVLLPPVEQPEPATWTGPEQVPVDTFSWDLPIKVLPTSPTLASYAALAPAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8L1</accession>
    <name>A0A4W2D8L1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8L1"/>
    <dbReference type="STRING" id="30522.A0A4W2D8L1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037391.1">
        <property type="protein sequence ID" value="ENSBIXP00000022323.1"/>
        <property type="gene ID" value="ENSBIXG00000025218.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="65"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="134" mass="15610" checksum="9F6EB05ED7D83769" modified="2019-09-18" version="1">MLNWWKRKNKIPMSVHVLERLLLVPSADPSLCADWFLGPLQLKVVRRPESSFCNTIHRQKMPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEFRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8P7</accession>
    <name>A0A4W2D8P7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 17">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="17">ATase</shortName>
            <ecNumber evidence="3 17">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 17">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:14907"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="19">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="20">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 17">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 17"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8P7"/>
    <dbReference type="STRING" id="30522.A0A4W2D8P7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037390.1">
        <property type="protein sequence ID" value="ENSBIXP00000022322.1"/>
        <property type="gene ID" value="ENSBIXG00000025217.1"/>
    </dbReference>
    <dbReference type="OMA" id="IRHFGVK"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000047">
        <property type="entry name" value="Amidophosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 20" id="KW-0408">Iron</keyword>
    <keyword evidence="12 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="21">
        <location>
            <begin position="12"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="539" mass="59873" checksum="57DA879F386EF550" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCD7</accession>
    <name>A0A4W2DCD7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCD7"/>
    <dbReference type="STRING" id="30522.A0A4W2DCD7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028327.1">
        <property type="protein sequence ID" value="ENSBIXP00000016406.1"/>
        <property type="gene ID" value="ENSBIXG00000020603.1"/>
    </dbReference>
    <dbReference type="OMA" id="LGHWKDR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071888">
        <property type="term" value="P:macrophage apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13317">
        <property type="entry name" value="PH_PLEKHO1_PLEKHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043448">
        <property type="entry name" value="PKHO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15871">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15871:SF2">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY O MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="18"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="489" mass="53267" checksum="5ED6CE00B755B69C" modified="2019-09-18" version="1">MEEEGVKEGGQKPRSAQTADKAGWIKKSSGGFLGLWKDRYLLLCQAQLLVYENEDEQKCVETVELGSYEKCQDLRALLKRKHRFILLRSPGNKVSDIKFQAPSGEEKESWIKALNEGINRGKNKAFDEVKVDKSCVLEHVTRDRVRRDQRRRPPTRVHLKEVANAASDGLSRLDLDVPDSGPPVLAPSNDVDAAQPRETPRPPMPPAKPSPAPETSSAGDRMETPVGQSAPAPVPASSEAHPESQEDLETPVVEDSDSEQPPNRILPDKLKVSWENPSPEEAPDSESAEPPQVPGAETSEAGPREGGKPPTPPPKILSEKLKASMSGMEASGPSAPGPAEVSVNGVDDSPEPLQSSQAAGPPGTPPKAATTSTTLPWDLQPQLHPRCSSLGDLLGEGPRRRRQPGEQLHRAQLEVKVASEKTEKLLNKVLGGESASVNAETLLSQAVEQLRQATQVLQEIRDLEEMNREAPGLREKRRELVTLYRRSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DE79</accession>
    <name>A0A4W2DE79_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Mast/stem cell growth factor receptor</fullName>
            <ecNumber evidence="23">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">KIT</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3CD. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC. Interacts with IL1RAP (independent of stimulation with KITLG/SCF). A mast cell-specific KITLG/SCF-induced interleukin-33 signaling complex contains IL1RL1, IL1RAP, KIT and MYD88.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Membrane</location>
            <topology evidence="29">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23 29">Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="23"/>
    <dbReference type="RefSeq" id="XP_027400321.1">
        <property type="nucleotide sequence ID" value="XM_027544520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DE79"/>
    <dbReference type="SMR" id="A0A4W2DE79"/>
    <dbReference type="STRING" id="30522.A0A4W2DE79"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037646.1">
        <property type="protein sequence ID" value="ENSBIXP00000022500.1"/>
        <property type="gene ID" value="ENSBIXG00000005180.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010623.1">
        <property type="protein sequence ID" value="ENSBIXP00005003277.1"/>
        <property type="gene ID" value="ENSBIXG00005009446.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894295"/>
    <dbReference type="CTD" id="3815"/>
    <dbReference type="GeneTree" id="ENSGT00940000155626"/>
    <dbReference type="OMA" id="CDSTNEY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042169">
        <property type="term" value="F:SH2 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005020">
        <property type="term" value="F:stem cell factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035234">
        <property type="term" value="P:ectopic germ cell programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038162">
        <property type="term" value="P:erythropoietin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038093">
        <property type="term" value="P:Fc receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006687">
        <property type="term" value="P:glycosphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="977" mass="109685" checksum="4B2719050883B7EF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEK4</accession>
    <name>A0A4W2DEK4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEK4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038002.1">
        <property type="protein sequence ID" value="ENSBIXP00000022730.1"/>
        <property type="gene ID" value="ENSBIXG00000025520.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="108" mass="11021" checksum="E926E29A2277FB91" modified="2019-09-18" version="1">MDWGKTLSSEQPRAADLASLPLISRLSRVSRARACLSGWVVPGSGSWDLGETPGVGSASGPGLGGQGMQTPMGSAPAFSLRIDLSPKPLPGGLWPPVKAPSLGTRSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGD0</accession>
    <name>A0A4W2DGD0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">XRN2-binding (XTBD) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CARF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGD0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050857.1">
        <property type="protein sequence ID" value="ENSBIXP00000023208.1"/>
        <property type="gene ID" value="ENSBIXG00000025748.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021859">
        <property type="entry name" value="XTBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11952">
        <property type="entry name" value="XTBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51827">
        <property type="entry name" value="XTBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XRN2-binding (XTBD)" evidence="3">
        <location>
            <begin position="73"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="172" mass="19196" checksum="F87F04D46B195C74" modified="2019-09-18" version="1">MRPPRPPRALQTNPPAQSRAAGAPLAAERWRSACREGSGRVRPLAVEGAMVGGEAAAAVEELISGVRRATDFAEQFRSYSESEKQWKARMEFILRHLPDYRDPPDGGGRLDQLLSLSMVWANHLFLGCSYNKDLLDKVMEMADGIEVEDLPQFTTRSELMKKCQPPESSPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIY4</accession>
    <name>A0A4W2DIY4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSBIXT00000043201.1">
        <property type="protein sequence ID" value="ENSBIXP00000026171.1"/>
        <property type="gene ID" value="ENSBIXG00000028269.1"/>
    </dbReference>
    <dbReference type="OMA" id="FMGLFKT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043379">
        <property type="entry name" value="ANKAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46464">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46464:SF1">
        <property type="entry name" value="ANKYRIN AND ARMADILLO REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="638"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="742"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1433" mass="161438" checksum="C9E897D01F85E4CB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLB1</accession>
    <name>A0A4W2DLB1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014094.1">
        <property type="protein sequence ID" value="ENSBIXP00000027218.1"/>
        <property type="gene ID" value="ENSBIXG00000028598.1"/>
    </dbReference>
    <dbReference type="OMA" id="LMRVKAK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="344" mass="39508" checksum="28C0A20A1EBC35C1" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQAGFRKGRGTRDQVANIRWIMEKVREFQKNIYFCFIDYAKAFDCVNHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTKDWFQIGKGACQGCILSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMRVKAKSEKAGLKLNIQKTKIMASGPTTSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTNVRLVKAMVFPVVMYGCESWIVKKAERRRIDAFGLWCWRRLLRLPWTARRSNQSILKEKIGPGISLEGMMLKLKLQYFGHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM86</accession>
    <name>A0A4W2DM86_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 207</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027394200.1">
        <property type="nucleotide sequence ID" value="XM_027538399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DM86"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049203.1">
        <property type="protein sequence ID" value="ENSBIXP00000019994.1"/>
        <property type="gene ID" value="ENSBIXG00000003982.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037488.1">
        <property type="protein sequence ID" value="ENSBIXP00005022929.1"/>
        <property type="gene ID" value="ENSBIXG00005025717.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890627"/>
    <dbReference type="CTD" id="131920"/>
    <dbReference type="GeneTree" id="ENSGT00390000006126"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039490">
        <property type="entry name" value="TMEM207"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36467">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36467:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="149" mass="16713" checksum="786ED2AFB1220513" modified="2019-09-18" version="1">MLRSRLLSDASVISKAGILCLPLFQLVLSDTPCEENEMCINYKDHYTNGWYIWFLLLIFLVALLCGTVIFCFQCCLRRLKNGPRRRTMAVFAVGDLDSVCGTEAAVTPTVGMHLHTQNPELFRAPCFGVLGPPPPYEETKIKPSSDLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DN81</accession>
    <name>A0A4W2DN81_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DN81"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042321.1">
        <property type="protein sequence ID" value="ENSBIXP00000025580.1"/>
        <property type="gene ID" value="ENSBIXG00000027658.1"/>
    </dbReference>
    <dbReference type="OMA" id="INSMCAL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="42956" checksum="C9314F01A9AAB247" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKAFDCVDHNKLWEILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTHCFQIGKGVRQGCILSPCLFNLYAEYIMRHAGLEEAQAGIKIAGRNINNLRYADDTTLMAESKEELKSLLMKVKEETEKIGLKLNIQKTKIMASGPITSWEIDEETVEKVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRVDSLEKTLMLGGIVGRRRRGRQRMRWLDGITDSMDVSLSELWELVMDREAWRAAIHGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP89</accession>
    <name>A0A4W2DP89_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neuropeptide Y receptor type 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">NPY-Y5 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">NPY5R</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399485.1">
        <property type="nucleotide sequence ID" value="XM_027543684.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399486.1">
        <property type="nucleotide sequence ID" value="XM_027543685.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399487.1">
        <property type="nucleotide sequence ID" value="XM_027543686.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399488.1">
        <property type="nucleotide sequence ID" value="XM_027543687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DP89"/>
    <dbReference type="STRING" id="30522.A0A4W2DP89"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043350.1">
        <property type="protein sequence ID" value="ENSBIXP00000026240.1"/>
        <property type="gene ID" value="ENSBIXG00000028359.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043675.1">
        <property type="protein sequence ID" value="ENSBIXP00005045455.1"/>
        <property type="gene ID" value="ENSBIXG00005005169.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893972"/>
    <dbReference type="CTD" id="4889"/>
    <dbReference type="GeneTree" id="ENSGT00940000161766"/>
    <dbReference type="OMA" id="RHHQDTH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001602">
        <property type="term" value="F:pancreatic polypeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001601">
        <property type="term" value="F:peptide YY receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003214">
        <property type="term" value="P:cardiac left ventricle morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003151">
        <property type="term" value="P:outflow tract morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099538">
        <property type="term" value="P:synaptic signaling via neuropeptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15398">
        <property type="entry name" value="7tmA_NPY5R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000393">
        <property type="entry name" value="NPY5_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF10">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01016">
        <property type="entry name" value="NRPEPTIDEY5R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="58"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="446" mass="50776" checksum="2A1113D30E7F0485" modified="2019-09-18" version="1">MDSELKDYYNKTSAGENNTAALRNSDFPVWDDYKSSVDDLQYFLIGLYTLVSLLGFMGNLLILMALMRKRNQKTTVNFLIGNLAFSDILVVLFCSPFTLTSVLLDQWMFGRVMCHIMPFLQCVTVLVSTLILISIAIVRYHMIKHPVSNHLTANHGYFLIATVWTLGFAMCSPLPVFHSLVELQETFGSALLSNRYLCVESWPSDAYRIAFTISLLLVQYILPLVCLTVSHTSVCRSISCGLSNKENQLEEKEMINLTLHPSQKSGPQVKLPSSQKWSYSFIRKHRRRYSKKTACVLPAPARPPLENRPGRLPEHAGSRKSPLPSSSKFIPGVPTCFEVKPEENSDVHEMRVNQSIMRIKKRSRSVFYRLTILILVFAVSWMPLHLFHVVTDFNDNLISNRHFKLVYCICHLLGMMSCCLNPILYGFLNNGIKADLRSLIHCLHMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DS35</accession>
    <name>A0A4W2DS35_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mab-21 like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MAB21L4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DS35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036672.1">
        <property type="protein sequence ID" value="ENSBIXP00000021830.1"/>
        <property type="gene ID" value="ENSBIXG00000024848.1"/>
    </dbReference>
    <dbReference type="OMA" id="VHCKHHS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF7">
        <property type="entry name" value="PROTEIN MAB-21-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="1">
        <location>
            <begin position="287"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="442" mass="48609" checksum="3CBF6ABFC0AA34F1" modified="2019-09-18" version="1">AVPAAMAVQVPLWHHYLQALRSRAASRAQEHQRTEDVVLTLLERAHALDPRFLVDYSRDLEAFQFALRSSEGPLDVEVPLWVDAEALVIEQMGAAEAGDGLGCCRLGLPKEAAGLERWKADDVFEASPEGSAGCCGHIVPSKVLRVLKDLLVAAVVYCKHHGLITPGSLSADSLREEQLGLSLRVSSGWRTLHFNMVPVVRKRLRVPALEGAQLVPGFPEGALRGIVRQEVALVPAGAELWRVSTDYLLTRLLGALGCLRGHRLDSLSILDRVNCESWRDGGRSPGLTFGHLKMVLLWAAALFPAPEDWAELQGAVYRPLVVLLCCLATRNLPHFLHPERNLLQDTGLDLGALYQRVERFASQPEAALRIHATHLGRSPPPRVGSGLRALLQLPAGEPAYWATAYFDVLLDKFQAFNIQDEQRVSAMQSVFLKTKTLGAEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSS8</accession>
    <name>A0A4W2DSS8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 SUMO-protein ligase EGR2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">E3 SUMO-protein transferase ERG2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Early growth response protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">EGR2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with HCFC1. Interacts with WWP2. Interacts with UBC9. Interacts with CITED1. Interacts (via phosphorylated form) with SFN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386590.1">
        <property type="nucleotide sequence ID" value="XM_027530789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSS8"/>
    <dbReference type="SMR" id="A0A4W2DSS8"/>
    <dbReference type="STRING" id="30522.A0A4W2DSS8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006542.1">
        <property type="protein sequence ID" value="ENSBIXP00000029628.1"/>
        <property type="gene ID" value="ENSBIXG00000011307.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028189.1">
        <property type="protein sequence ID" value="ENSBIXP00005016714.1"/>
        <property type="gene ID" value="ENSBIXG00005020273.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885457"/>
    <dbReference type="CTD" id="1959"/>
    <dbReference type="GeneTree" id="ENSGT00940000158394"/>
    <dbReference type="OMA" id="QCQRELH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035904">
        <property type="term" value="P:aorta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035284">
        <property type="term" value="P:brain segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021612">
        <property type="term" value="P:facial nerve structural organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031643">
        <property type="term" value="P:positive regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014040">
        <property type="term" value="P:positive regulation of Schwann cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030278">
        <property type="term" value="P:regulation of ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021660">
        <property type="term" value="P:rhombomere 3 formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021659">
        <property type="term" value="P:rhombomere 3 structural organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021666">
        <property type="term" value="P:rhombomere 5 formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021665">
        <property type="term" value="P:rhombomere 5 structural organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007622">
        <property type="term" value="P:rhythmic behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014037">
        <property type="term" value="P:Schwann cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035914">
        <property type="term" value="P:skeletal muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000837">
        <property type="entry name" value="E3 SUMO-protein ligase EGR2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000324">
        <property type="entry name" value="Early growth response protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000419">
        <property type="entry name" value="Early growth response protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF54">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE EGR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="9 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 19" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 18 19" id="KW-0863">Zinc-finger</keyword>
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</entry>
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        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06730">
        <property type="entry name" value="FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="26">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33878" checksum="8F9D38DDD0C5C1D3" modified="2019-09-18" version="1">MLRRSLENRDAQTRQLQDAVTNVEKHFGELCQIFAAYVRKTARLRDKADLLVNEINVYASTETPHLKQGLKNFADEFAKLQDYRQAEVERLEAKVVEPLKAYGTIVKMKRDDLKATLTARNREAKQLTQLERTRQRNPSDRHVISQAETELQRATMDATRTTRHLEETIDNFEKQKIKDIKTIFSEFITIEMLFHGKALEVYTAAYQNIQKIDEEEDLEVFRHSLYPQDYSSRLDIVRANSKSPLQRSLSAKCVSGTGQVLTCRLRKDHQTEDDDEEDEDLDVTEEENLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E146</accession>
    <name>A0A4W2E146_BOBOX</name>
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        <submittedName>
            <fullName evidence="5">Coiled-coil domain containing 92</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCDC92</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027422808.1">
        <property type="nucleotide sequence ID" value="XM_027567007.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027567008.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027422810.1">
        <property type="nucleotide sequence ID" value="XM_027567009.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2E146"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044394.1">
        <property type="protein sequence ID" value="ENSBIXP00000032865.1"/>
        <property type="gene ID" value="ENSBIXG00000015557.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023089.1">
        <property type="protein sequence ID" value="ENSBIXP00005036048.1"/>
        <property type="gene ID" value="ENSBIXG00005017440.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907601"/>
    <dbReference type="CTD" id="80212"/>
    <dbReference type="GeneTree" id="ENSGT00430000031027"/>
    <dbReference type="OMA" id="AHRIHHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040370">
        <property type="entry name" value="CCDC74A/CCDC74B/CCDC92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039496">
        <property type="entry name" value="CCDC92/74_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14882">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 74A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14882:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14916">
        <property type="entry name" value="CCDC92"/>
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    </dbReference>
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        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
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        </location>
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        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="315" mass="35018" checksum="D543FB26110CDAE9" modified="2019-09-18" version="1">MAATNLENQLHSAQKNLLFLQREHASTLKGLHAEIRRLQQHCTDLTYELTVKSSDQTGDGASRSSELKKRCEDLEAQLKLKEDENTELLKELEQKNAMITVLENTIKERERKYLEELKVKSHKLGVLTSELEQRAGTIAYLTSQLHATKRKLLSAGGTSDGSPAGSPALASYKPAPPKDRLPETPRRRMKKSLSAPLHPEFEEVYRFGAESRKLLLREPVDAMPDPTPFLLARESAEVHLIKERPLVIPPIASDRSAGEQSSPAREKPHKAHVGVAHRIHHVAPAQAPPEVETLAVDQVNGGKVVRKHSGTDRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1B8</accession>
    <name>A0A4W2E1B8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein canopy homolog 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the canopy family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E1B8"/>
    <dbReference type="STRING" id="30522.A0A4W2E1B8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003652.1">
        <property type="protein sequence ID" value="ENSBIXP00000030571.1"/>
        <property type="gene ID" value="ENSBIXG00000012594.1"/>
    </dbReference>
    <dbReference type="OMA" id="GQDKACL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15382">
        <property type="entry name" value="CTG4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15382:SF2">
        <property type="entry name" value="PROTEIN CANOPY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021506190" description="Protein canopy homolog 3" evidence="10">
        <location>
            <begin position="38"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3456" evidence="11">
        <location>
            <begin position="48"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="215"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="276" mass="30743" checksum="F60F6DF4D72B914B" modified="2019-09-18" version="1">MEPLPEPASHRLLLLSLLLLLLLLLPAPELGPRQARAEDTDWVRLPSKCEVCKYVAVELKSAFEETGKTKEVIDTGYGILDRKASGVKYTKSDLRLIEVTETICKRLLDYSLHKERTGSNRFAKGMSETFETLHNLVHKGVKVVMDIPYELWNETSAEVADLKKQCDVLVEEFEEVIEDWYRNHQEEDLTQFLCANHVLKGKDASCLAEQWSGKKGDTAALGGKKSKKKSGRAKGLGGGSSKQRKELGDLDGDPSPEEDEGIQKASPLTHSPPDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2W2</accession>
    <name>A0A4W2E2W2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Complement factor B</fullName>
            <ecNumber evidence="6">3.4.21.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">C3/C5 convertase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">CFB</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.</text>
    </comment>
    <comment type="function">
        <text evidence="23">Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases. CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases.</text>
    </comment>
    <comment type="function">
        <text evidence="22">Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway. Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b. As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways. When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.</text>
            <dbReference type="EC" id="3.4.21.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Catalytic component of the C3 convertase of the alternative complement pathway, also named C3bBb, composed of complement factor B Bb and complement C3b. Catalytic component of the C5 convertase of the alternative complement pathway, also named C3bBb3b, composed of complement factor B Bb and additional molecules of complement C3b. Interacts to CFP; this interaction contributes to the stabilization of the active C3-convertase enzyme complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="24">Monomer. Interacts with complement C3b; this interaction is dependent on the presence of Mg(2+).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="27">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.47" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E2W2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004028.1">
        <property type="protein sequence ID" value="ENSBIXP00000030443.1"/>
        <property type="gene ID" value="ENSBIXG00000006206.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01470">
        <property type="entry name" value="vWA_complement_factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000052">
        <property type="entry name" value="Complement factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000056">
        <property type="entry name" value="Complement factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000019">
        <property type="entry name" value="Complement factor b,-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011360">
        <property type="entry name" value="Compl_C2_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393:SF1">
        <property type="entry name" value="COMPLEMENT FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001154">
        <property type="entry name" value="Compl_C2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="17 27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 28" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11 28" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="15 28" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12 29" id="KW-0732">Signal</keyword>
    <keyword evidence="10 27" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021458431" description="Complement factor B" evidence="29">
        <location>
            <begin position="26"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="32">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="32">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="32">
        <location>
            <begin position="163"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="30">
        <location>
            <begin position="270"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="31">
        <location>
            <begin position="477"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="26">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="26">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="26">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00093327"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093434"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093516"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093582"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR001154-1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030443.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="761" mass="85356" checksum="CDBC557515519CF4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3N9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3N9"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000014">
        <property type="entry name" value="IQ motif containing F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000015">
        <property type="entry name" value="IQ motif containing F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039887">
        <property type="entry name" value="IQCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21633:SF4">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21633">
        <property type="entry name" value="IQ MOTIF CONTAINING F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="163" mass="19507" checksum="A9EDAEED05156B83" modified="2019-09-18" version="1">MGVRFCKDGHVIQIIIENKEEVTMKKMKEQQKKKEKTKNGRRVIAAKKIQAWWRGTLVRRTLLHAALSTWIIQSWWRLTKDRLLQKKRRAALSDYALRERAVVKLQSLVRMWRIHWRYCQVLNAIYVIQCHWQCHNCQTCALLRGHCVVTATHLQFHIEIINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E603</accession>
    <name>A0A4W2E603_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">E3 ubiquitin-protein ligase RNF144B</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">RING finger protein 144B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RNF144B</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Plays a crucial role in maintaining the genomic stability by controlling the degradation of multiple proteins involved in mitotic progression and DNA damage. Regulates epithelial homeostasis by mediating degradation of CDKN1A and isoform 2 of TP63. Plays a regulatory role in innate immunity by negatively regulating IRF3 activation and IFN-beta production. Mechanistically, inhibits TBK1 phosphorylation and 'Lys-63'-linked polyubiquitination independently of its E3 ligase activity. Alternatively, promotes 'Lys-27' and 'Lys-33'-linked ubiquitination of IFIH1/MDA5, promoting selective autophagic degradation of IFIH1/MDA5 to inhibit antiviral response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with UBE2L3, UBE2L6 and LCMT2, as well as with BAX. Interacts with TBK1; this interaction inhibits TBK1 phosphorylation and 'Lys-63'-linked polyubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the RBR family. RNF144 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E603"/>
    <dbReference type="STRING" id="30522.A0A4W2E603"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014387.1">
        <property type="protein sequence ID" value="ENSBIXP00000027135.1"/>
        <property type="gene ID" value="ENSBIXG00000028744.1"/>
    </dbReference>
    <dbReference type="OMA" id="CPDMACR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20367">
        <property type="entry name" value="BRcat_RBR_RNF144B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16778">
        <property type="entry name" value="mRING-HC-C4C4_RBR_RNF144B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20369">
        <property type="entry name" value="Rcat_RBR_RNF144B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000010">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="259"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="27"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="304" mass="33697" checksum="F0E549E38AA377F8" modified="2019-09-18" version="1">MGSVGRLHCLTMTAAENPTPGDLALAPLVTCKLCLCEQSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVATSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPGILPTEHGTLFGTETDAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDRGPCRNKLGHSRASVMWNRTQVVGILVGLGIIALVTSPLLLLASPCIICCVCKSCRGKKKKHDPSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E648</accession>
    <name>A0A4W2E648_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G2/mitotic-specific cyclin-B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CCNB1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for the control of the cell cycle at the G2/M (mitosis) transition.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3. Interacts with RFPL4A and UBE2A. Interacts with INCA1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E648"/>
    <dbReference type="SMR" id="A0A4W2E648"/>
    <dbReference type="STRING" id="30522.A0A4W2E648"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043908.1">
        <property type="protein sequence ID" value="ENSBIXP00000032236.1"/>
        <property type="gene ID" value="ENSBIXG00000014751.1"/>
    </dbReference>
    <dbReference type="OMA" id="MATRNHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20565">
        <property type="entry name" value="CYCLIN_CCNB1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000027">
        <property type="entry name" value="G2/mitotic-specific cyclin-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048026">
        <property type="entry name" value="CCNB1_first_cyclin-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 10" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="201"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="13">
        <location>
            <begin position="294"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="298"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="427" mass="47658" checksum="D74D586F0FCD610D" modified="2019-09-18" version="1">MALRITRNTKISAENKAKISMAGAKRVPVAAVATSKPGLRPRTALGDIGNKVSEQPQAKLPLKKEAKTLASGKVTAKKVPKPLEKAPVPVPEPQPEPEPEPEHVKEDKLSPEPILVDTPSPSPMETSGCAPAEEYLCQAFSDVILAVSDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVKPKYLMGREVTGNMRAILIDWLVQVQIKFRLLQETMYMTVSIIDRFMQDTYVPKKMLQLVGVTAMFVASKYEEMYPPEIGDFAFVTDNTYTKFQIRQMEMKILRALNFSLGRPLPLHFLRRASKIGEVDVELHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKVLDNGEWTPTLQHYLSYTEESLLVVMQHLAKNVVMVNRGLTKHMTIKNKYATSKHAKISTLAQLNSALVQDLAKAVAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6C3</accession>
    <name>A0A4W2E6C3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Jumonji</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Jumonji/ARID domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">JARID2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts as an accessory subunit for the core PRC2 (Polycomb repressive complex 2) complex, which mediates histone H3K27 (H3K27me3) trimethylation on chromatin. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells, thereby playing a key role in stem cell differentiation and normal embryonic development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. Does not have histone demethylase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Associates with the PRC2 complex, which consists of the core components EED, EZH1 or EZH2, SUZ12, and RBBP4, and various combinations of accessory subunits including AEBP2, JARID2, PHF19, MTF2 and EPOP. Found in a monomeric PRC2.2 (class 2) complex consisting of at least SUZ12, RBBP4, AEBP2 and JARID2. Facilitates nucleosome binding of the PRC2 complex. Interacts with SUZ12 (via C2H2-type zinc finger domain); the interaction is direct; competes with EPOP for SUZ12 binding. Interacts with histone methyltransferases EHMT1/GLP1 and EHMT2/G9a. Interacts with GATA4 (via the N-terminal region). Interacts with NKX2-5 (via the C-terminal region). Interacts with RB1. Interacts with ZNF496. Interacts with ESRRB. Interacts with DDX18; this interaction inhibits the PRC2 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027380036.1">
        <property type="nucleotide sequence ID" value="XM_027524235.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380037.1">
        <property type="nucleotide sequence ID" value="XM_027524236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6C3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013946.1">
        <property type="protein sequence ID" value="ENSBIXP00000027251.1"/>
        <property type="gene ID" value="ENSBIXG00000028524.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042600.1">
        <property type="protein sequence ID" value="ENSBIXP00005026380.1"/>
        <property type="gene ID" value="ENSBIXG00005005348.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881297"/>
    <dbReference type="CTD" id="3720"/>
    <dbReference type="GeneTree" id="ENSGT00940000159220"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16870">
        <property type="entry name" value="ARID_JARD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000007">
        <property type="entry name" value="Jumonji, AT rich interactive domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000005">
        <property type="entry name" value="Jumonji, AT-rich interactive domain 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF113">
        <property type="entry name" value="PROTEIN JUMONJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjN" evidence="15">
        <location>
            <begin position="557"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="14">
        <location>
            <begin position="621"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="16">
        <location>
            <begin position="884"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="43"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="357"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1108"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1208"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070290"/>
        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
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        </source>
    </evidence>
    <sequence length="1236" mass="137282" checksum="373A36022E5C8E9C" modified="2019-09-18" version="1"> </sequence>
</entry>
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    <accession>A0A4W2E8Y4</accession>
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        <submittedName>
            <fullName evidence="6">CAP-Gly domain containing linker protein 3</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">CLIP3</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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        <location>
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        <location>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
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        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033278.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="547" mass="59652" checksum="D27072CFDD0030D9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E953</accession>
    <name>A0A4W2E953_BOBOX</name>
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        <recommendedName>
            <fullName evidence="10">Ataxin-7-like protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">SAGA-associated factor 11 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 14" type="primary">ATXN7L3</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. Within the complex, it is required to recruit USP22 and ENY2 into the SAGA complex. Regulates H2B monoubiquitination (H2Bub1) levels. Affects subcellular distribution of ENY2, USP22 and ATXN7L3B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ENY2, ATXN7, ATXN7L3, and USP22 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with ENY2 and USP22.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal SGF11-type zinc-finger domain together with the C-terminal catalytic domain of USP22 forms the 'catalytic lobe' of the SAGA deubiquitination module.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The long N-terminal helix forms part of the 'assembly lobe' of the SAGA deubiquitination module.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SGF11 family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000016113.1"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03047">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
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        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051078">
        <property type="entry name" value="SGF11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46367">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46367:SF1">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
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            <end position="270"/>
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        <source>
            <dbReference type="PROSITE" id="PS51505"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="354" mass="39603" checksum="B6E79A9776C63E43" modified="2019-09-18" version="1">MKMEEMSLSGLDNSKLEAIAQEIYADLVEDSCLGFCFEVHRAVKCGYFFLDDTDPESMKDFEIVDQPGLDIFGQVFNQWKSKECVCPNCSRSIAASRFAPHLEKCLGMGRNSSRIANRRIANSNNMNKSESDQEDNDDINDNDWSYGSEKKAKKRKSDKLWYLPFQNPNSPRRSKSLKHKNGELSNSDPFKYNNSTGISYETLGPEELRSLLTTQCGVISEHTKKMCTRSLRCPQHTDEQRRTVRIYFLGSSAVLPEVESSLDNDSFDMTDSQALISRLQWDGSSDLSPSDSGSSKTSENQGWGLGTNSSESRKTKKKKSHLSLVGTASGLGSNKKKKPKPPAPPTPSIYDDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9K1</accession>
    <name>A0A4W2E9K1_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 42</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">TMEM42</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31965:SF1">
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        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
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            <begin position="65"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="127"/>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
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    <evidence type="ECO:0000313" key="2">
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="159" mass="16956" checksum="77F43F40E0AEAF82" modified="2019-09-18" version="1">MAGRLLPGGGGVCAAAYPNTSAGFPPHLQAGAMRRRFWGVFNCLCAGAFGALAAASAKLAFGREVNVGFCVLGIIMMATTNSLMWTFFSRGLSLSMSSAIASVTVTFSNILNSAFLGFVLYGECQEVLWWGGVFLILCGLTLIHRELPPPRKPLPHKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9R9</accession>
    <name>A0A4W2E9R9_BOBOX</name>
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        <recommendedName>
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        <name evidence="13" type="primary">DFFB</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of DFFA and DFFB. Interacts with H1-1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000003375.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DNA fragmentation factor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
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        <property type="entry name" value="DFF40"/>
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    </dbReference>
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        <property type="entry name" value="His-Me_finger_sf"/>
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        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAD"/>
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    </dbReference>
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        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
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        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="341" mass="38958" checksum="708AA3822ED8EEAF" modified="2019-09-18" version="1">MSAVLRKPKTFKLRSLHSEKKFGVAGRSCEEVLRKGCQRLQLPIPGSRLCLYEDGTELTGDYFWSAPDNSELVLLTAGQTWQGFVSDISRFLSVFQEPHAGVIQAARQLLWDERAPLRQKLLADLLGTVSENIAAETRAEDPPWFEGLESRFRSKSGYLRYSCESRIRSYLREVTSGASLVGAEAREEYLRLVGSMQQKLQAAQYNSSYFDRGAKAGRRLCTPEGWFSCQGPFDVDDCTSRHSINPYSNRESRVLFSTWNLDHVIEKKRVVVPALAAAVHDAEGREVDWEYFYRLLFTLENLKLVHIACHKKTTHKLHCDPSRVYCTPAAPRRKRHARQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAD2</accession>
    <name>A0A4W2EAD2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with hair keratins.</text>
    </comment>
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        <property type="entry name" value="KERATIN ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KERATIN-ASSOCIATED PROTEIN 4-11-RELATED"/>
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    <keyword evidence="4" id="KW-0416">Keratin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00003327"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011662"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="306" mass="32027" checksum="86D902AAA4FBEDE0" modified="2019-09-18" version="1">MVSSCCGSVCSDQSCGRSLCQETCCPPSCCQTTCCRTTCCRPSCGVSSCCRPVCCHCGSSCCRPTCCISSCCRPHCYRPSCCGSSCGSSCCRPQCCQPVCCQPTCPRPTCCISSCYRPSCCGSSCGSSCCRPTCCISSCCRPRCCQSVCCQPTCSRISSCCRPSCCGSSCCLPTCCISSCCRPQCCQPVCCQPTCPRPTCCISSCCRPSCCGSSCGSSCCRPTCCISSCCRPTCCISSCCRPRCCQSVCCQPTCSRISSCCRPSCCGSSCCLRPVCGRVSCHTTCYRPTCVISTCPRPVCCPSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECN4</accession>
    <name>A0A4W2ECN4_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="177"/>
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        <source>
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            <dbReference type="Ensembl" id="ENSBIXP00000037281.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="465" mass="53878" checksum="E8C05F1696F85514" modified="2019-09-18" version="1">MQKNEMAVWGGLTNSCEKKRSENKGEKERYKHLNAEFQRIPRRDKKAFFSDQCKEIEENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTELYKKDLHDQDNHDGVITHLVPDILECEVKWALESITMNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKIQQWPHDWKRSVFIPIPKKGNAKECSNYHTIALISHARKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANIRWITGKAREFQKNIYFYFIDYAKAFDCVNHNKLWKILKEIGIPDHLICLLRNLYAGQEATVRTGHGTIDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIARRNINNLTYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASRPTSSREINGKQWKQCQILFFWAPESLQMVTAAMKLKDAYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ED21</accession>
    <name>A0A4W2ED21_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). Participates in synaptic plasticity for learning and memory formation. Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium. Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators.</text>
    </comment>
    <comment type="function">
        <text evidence="29">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Heterotetramer. Forms heterotetrameric channels composed of two GluN1/zeta subunits (GRIN1), and two identical GluN2/epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) or GluN3 subunits (GRIN3A or GRIN3B) (in vitro). In vivo, the subunit composition may depend on the expression levels of the different subunits. Interacts with PDZ domains of PATJ and DLG4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19 29">Postsynaptic cell membrane</location>
            <topology evidence="19 29">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2ED21"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023034.1">
        <property type="protein sequence ID" value="ENSBIXP00000034813.1"/>
        <property type="gene ID" value="ENSBIXG00000017903.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06378">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13718">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000626">
        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000397">
        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000007">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 29" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 29" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 29" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 29" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16 29" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="14 29" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 29" id="KW-0732">Signal</keyword>
    <keyword evidence="10 29" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 29" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027145984" description="Glutamate receptor" evidence="29">
        <location>
            <begin position="26"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="628"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="657"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="846"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="31">
        <location>
            <begin position="431"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="32">
        <location>
            <begin position="474"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="900"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="972"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1211"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="902"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="978"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="990"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="30">
        <location>
            <begin position="1072"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="1090"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="30">
        <location>
            <begin position="1217"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="541"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="717"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="718"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="759"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="27">
        <location>
            <position position="690"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="27">
        <location>
            <position position="723"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="773"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00056249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00061683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00063280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1333" mass="143684" checksum="AD5A6F548EC5A4FD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ED91</accession>
    <name>A0A4W2ED91_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">E3 ubiquitin-protein ligase TRIM9</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">RING-type E3 ubiquitin transferase TRIM9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Tripartite motif-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">TRIM9</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with SNAP25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027409534.1">
        <property type="nucleotide sequence ID" value="XM_027553733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ED91"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028470.1">
        <property type="protein sequence ID" value="ENSBIXP00000037147.1"/>
        <property type="gene ID" value="ENSBIXG00000020670.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050252.1">
        <property type="protein sequence ID" value="ENSBIXP00005040654.1"/>
        <property type="gene ID" value="ENSBIXG00005023384.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900200"/>
    <dbReference type="CTD" id="114088"/>
    <dbReference type="GeneTree" id="ENSGT00940000154071"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19843">
        <property type="entry name" value="Bbox1_TRIM9_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19826">
        <property type="entry name" value="Bbox2_TRIM9_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16755">
        <property type="entry name" value="RING-HC_TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12889">
        <property type="entry name" value="SPRY_PRY_TRIM67_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000178">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000168">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.40:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000329">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000017">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049582">
        <property type="entry name" value="TRIM9_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17 26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="27">
        <location>
            <begin position="10"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="28">
        <location>
            <begin position="163"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="28">
        <location>
            <begin position="224"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="31">
        <location>
            <begin position="374"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="440"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="29">
        <location>
            <begin position="525"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="288"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00079716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="718" mass="80228" checksum="E4AF0A88CD4A8890" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHL0</accession>
    <name>A0A4W2EHL0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">B-cell CLL/lymphoma 6 member B protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Bcl6-associated zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BCL6B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Associates with BCL6 through the BTB domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EHL0"/>
    <dbReference type="SMR" id="A0A4W2EHL0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016449.1">
        <property type="protein sequence ID" value="ENSBIXP00000032003.1"/>
        <property type="gene ID" value="ENSBIXG00000014450.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000097">
        <property type="entry name" value="B-cell CLL/lymphoma 6 member B protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000105">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000289">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001344">
        <property type="entry name" value="Zinc finger protein 16 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF25">
        <property type="entry name" value="ZGC:110075 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="38"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="143"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="267"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="473" mass="51055" checksum="2327DE5E051ADA16" modified="2019-09-18" version="1">MGSTATPEGALGYVREFTRHSSDVLGNLNELRLRGILTDVTLLVGGQPLRAHKAVLIACSGFFYSIFRGRAGVGVDMLSLPGGPEAGGFAPLLDFMYTSRLRLSPATAPAVLAAATYLQMEHVVQACHRFIQASYEPLGISLRPLEAEPPTPPTAPPPGSPRRSEGHPDPPTESRSCSQGPPSPHSPDPKACNWKKYKFIILNSQASQAGSLARESSGQLCSQARLPSGDEASSSSSSEEGPISSPQSRLSPTAATVQFKCEAPVNTRHQLTPQAPETTGSPSGRAHPPPGGEFFSCQNCEAVAGCSSGLDPVASGDEDKPYKCQLCRSAFRYKGNLASHRTVHTGEKPYHCSICGARFNRPANLKTHSRIHSGEKPYKCETCGSRFVQVAHLRAHVLIHTGEKPYPCPTCGTRFRHLQTLKSHVRIHTGEKPYHCDPCGLHFRHKSQLRLHLRQKHGAATNTKVHYHILGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJ12</accession>
    <name>A0A4W2EJ12_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox protein MIXL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Homeodomain protein MIX</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">MIX1 homeobox-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Mix.1 homeobox-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJ12"/>
    <dbReference type="STRING" id="30522.A0A4W2EJ12"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028407.1">
        <property type="protein sequence ID" value="ENSBIXP00000037113.1"/>
        <property type="gene ID" value="ENSBIXG00000020635.1"/>
    </dbReference>
    <dbReference type="OMA" id="HHSACET"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000329">
        <property type="entry name" value="Mix paired-like homeobox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042917">
        <property type="entry name" value="MIXL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47656">
        <property type="entry name" value="HOMEOBOX PROTEIN MIXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47656:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN MIXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 15 16" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 15 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="18">
        <location>
            <begin position="89"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="91"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="244" mass="26246" checksum="99603B91F416EB21" modified="2019-09-18" version="1">MAAAESSQLQFEEIAAPPAYLPAHPGGAFLPPPNPNAGLLLARPSGSAPELGAPVAFAGFLGRGPGPAAPPHAGLGPPAPPKGAAVSSASQRRKRTSFRPEQLQLLELVFRRTMYPDIHLRERLAALTLLPESRIQFLSSLLQVWFQNRRAKSRRQSGKPFQPSARPELFLHHSACETEAKYLKAQPPLEVDVNCLPDPNKVGGGISDSSSQSQNFETCSPLSEDIGSKLDSWEEHIFSAFGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EK05</accession>
    <name>A0A4W2EK05_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Coiled-coil domain-containing protein 104</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CFAP36</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May act as an effector for ARL3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with ARL3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFAP36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411139.1">
        <property type="nucleotide sequence ID" value="XM_027555338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EK05"/>
    <dbReference type="STRING" id="30522.A0A4W2EK05"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021121.1">
        <property type="protein sequence ID" value="ENSBIXP00000034112.1"/>
        <property type="gene ID" value="ENSBIXG00000007913.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000021128.1">
        <property type="protein sequence ID" value="ENSBIXP00000033977.1"/>
        <property type="gene ID" value="ENSBIXG00000007913.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023690.1">
        <property type="protein sequence ID" value="ENSBIXP00005013696.1"/>
        <property type="gene ID" value="ENSBIXG00005017832.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901220"/>
    <dbReference type="CTD" id="112942"/>
    <dbReference type="GeneTree" id="ENSGT00390000012785"/>
    <dbReference type="OMA" id="DWYIPIL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1520.10:FF:000001">
        <property type="entry name" value="Cilia- and flagella-associated protein 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1520.10">
        <property type="entry name" value="ADP-ribosylation factor-like 2-binding protein, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042541">
        <property type="entry name" value="BART_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038888">
        <property type="entry name" value="CFAP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532">
        <property type="entry name" value="PHOSPHODIESTERASE HL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="17">
        <location>
            <begin position="10"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="292"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="343" mass="39626" checksum="C76F17C20FFAC1A5" modified="2019-09-18" version="1">MAAEEEDEVEWVVESIAGFLRGPDWSIPILDFVEQKCEVFDDEEESKLTYTEIHQEYKELVEKLLETYLKEIGINEDQFQEACTSPLAKTRTSQAILQPVLAAEDFTIFKAMMVEKNTEMQLQAIRIIQERNGVLPDCLTDGSDVVSDLEQEEMKILKEVLRKSKEEYDQEEERKRKKQLSEAKTEEHPMQANETAKMSNSQGDGEHFAHPASEVKVHFANQSVQPLTRKLEMLPETSSLPQKGLKIPGFEHASMEGPIANLSTLRTEELRQREHYLKQKRDKLMSMRKDMKIKQNQTSEQKGKPAGEVEEMTEKPEMTAEEKQTLLKRRLLAEKLKEEVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKM5</accession>
    <name>A0A4W2EKM5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113905166</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKM5"/>
    <dbReference type="STRING" id="30522.A0A4W2EKM5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023754.1">
        <property type="protein sequence ID" value="ENSBIXP00000035120.1"/>
        <property type="gene ID" value="ENSBIXG00000006673.1"/>
    </dbReference>
    <dbReference type="OMA" id="SFCRLNA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="316" mass="35017" checksum="865033AEC1779DBA" modified="2019-09-18" version="1">MAAKNFTVVTEFIILGLTDNTELKVVLFVLFLVIYAITLVGNLGMMIFLIQVTPKLRTLMYFFLSCLSSVDACYSSVIAPKLLISFLVVRQTISFSACIVQHLFFGVFITIEGFLLSMMAYDYYVAIVNPLLYAAAMSKRKSVGLVTGSLIGGMINSLTHTISFGRLSFCRSNVISHFFCDVPPLLKLSCSDTSMNELLLLTSSGVIAMATFLIVIISYTFIAVAILRIRSAAGRQKAFSTCASHLTTVTIFYSTLSFNYIQPSSQYSVEQEKVVSVFYTLVIPMLNPLIYSLRNKEVKAARQKREGRSSIPGRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELX2</accession>
    <name>A0A4W2ELX2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">TPX2 microtubule nucleation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TPX2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPX2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELX2"/>
    <dbReference type="SMR" id="A0A4W2ELX2"/>
    <dbReference type="STRING" id="30522.A0A4W2ELX2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029168.1">
        <property type="protein sequence ID" value="ENSBIXP00000037451.1"/>
        <property type="gene ID" value="ENSBIXG00000021027.1"/>
    </dbReference>
    <dbReference type="OMA" id="GRHTVSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015128">
        <property type="entry name" value="Aurora-A-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027330">
        <property type="entry name" value="TPX2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF44">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09041">
        <property type="entry name" value="Aurora-A_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12214">
        <property type="entry name" value="TPX2_importin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aurora-A binding" evidence="15">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 central" evidence="16">
        <location>
            <begin position="365"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 C-terminal" evidence="14">
        <location>
            <begin position="543"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 C-terminal" evidence="14">
        <location>
            <begin position="660"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="87"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="662"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="747" mass="86324" checksum="D382E623F5EB6C0F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EM35</accession>
    <name>A0A4W2EM35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC113903090</name>
        <name evidence="13" type="synonym">EDEM2</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2. Seems to recognize and bind to exposed hydrophobic regions in target proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027414525.1">
        <property type="nucleotide sequence ID" value="XM_027558724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EM35"/>
    <dbReference type="SMR" id="A0A4W2EM35"/>
    <dbReference type="STRING" id="30522.A0A4W2EM35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029975.1">
        <property type="protein sequence ID" value="ENSBIXP00000037794.1"/>
        <property type="gene ID" value="ENSBIXG00000021470.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043975.1">
        <property type="protein sequence ID" value="ENSBIXP00005027188.1"/>
        <property type="gene ID" value="ENSBIXG00005001632.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903090"/>
    <dbReference type="GeneTree" id="ENSGT00940000159233"/>
    <dbReference type="OMA" id="HNYHRVW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904154">
        <property type="term" value="P:positive regulation of retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000015">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611143" description="alpha-1,2-Mannosidase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="517"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="538"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="578" mass="64859" checksum="C98008ED8B7C3B95" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ENA2</accession>
    <name>A0A4W2ENA2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">IQ calmodulin-binding motif-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">IQCB1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in ciliogenesis. The function in an early step in cilia formation depends on its association with CEP290/NPHP6. Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2 and BBS5 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating CEP290/NPHP6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ENA2"/>
    <dbReference type="SMR" id="A0A4W2ENA2"/>
    <dbReference type="STRING" id="30522.A0A4W2ENA2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048475.1">
        <property type="protein sequence ID" value="ENSBIXP00000038443.1"/>
        <property type="gene ID" value="ENSBIXG00000022283.1"/>
    </dbReference>
    <dbReference type="OMA" id="DDYIRLH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032391">
        <property type="term" value="C:photoreceptor connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000048">
        <property type="entry name" value="IQ motif containing B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000058">
        <property type="entry name" value="IQ motif containing B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028765">
        <property type="entry name" value="IQCB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15673">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15673:SF2">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="598" mass="68508" checksum="8B21F6FE56D4AC98" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETV1</accession>
    <name>A0A4W2ETV1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Otogelin like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">OTOGL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the otogelin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2ETV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049776.1">
        <property type="protein sequence ID" value="ENSBIXP00000040204.1"/>
        <property type="gene ID" value="ENSBIXG00000024261.1"/>
    </dbReference>
    <dbReference type="OMA" id="LNGTMKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046373">
        <property type="term" value="P:L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036195">
        <property type="entry name" value="AbfB_ABD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
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        <property type="entry name" value="Fn1-VW_OTOGL"/>
    </dbReference>
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        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058754">
        <property type="entry name" value="OTOGL-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
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        <property type="entry name" value="TIL_OTOGL_Mucin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF225">
        <property type="entry name" value="OTOGELIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25960">
        <property type="entry name" value="Fn1-VW_OTOGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25961">
        <property type="entry name" value="OTOGL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25962">
        <property type="entry name" value="TIL_OTOGL_Mucin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110221">
        <property type="entry name" value="AbfB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
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        <location>
            <begin position="121"/>
            <end position="297"/>
        </location>
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    <feature type="domain" description="VWFD" evidence="8">
        <location>
            <begin position="481"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="8">
        <location>
            <begin position="946"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="8">
        <location>
            <begin position="1534"/>
            <end position="1724"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="7">
        <location>
            <begin position="2261"/>
            <end position="2353"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="2282"/>
            <end position="2331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2353" mass="264506" checksum="56F0F31A05F29D86" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV82</accession>
    <name>A0A4W2EV82_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">ADAMTS like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ADAMTSL4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027390316.1">
        <property type="nucleotide sequence ID" value="XM_027534515.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027390327.1">
        <property type="nucleotide sequence ID" value="XM_027534526.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027390335.1">
        <property type="nucleotide sequence ID" value="XM_027534534.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EV82"/>
    <dbReference type="STRING" id="30522.A0A4W2EV82"/>
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        <property type="protein sequence ID" value="ENSBIXP00000040684.1"/>
        <property type="gene ID" value="ENSBIXG00000006685.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039228.1">
        <property type="protein sequence ID" value="ENSBIXP00005043138.1"/>
        <property type="gene ID" value="ENSBIXG00005026752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887397"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161136"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002064">
        <property type="term" value="P:epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0070285">
        <property type="term" value="P:pigment cell development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of apoptotic process"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000111">
        <property type="entry name" value="ADAMTS-like protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000023">
        <property type="entry name" value="Thrombospondin type-1 domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000039">
        <property type="entry name" value="thrombospondin type-1 domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF144">
        <property type="entry name" value="ADAMTS-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
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    </dbReference>
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        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611193" evidence="6">
        <location>
            <begin position="25"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="7">
        <location>
            <begin position="1025"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1024"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1070" mass="116285" checksum="43E8B4EE3D13B326" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVV6</accession>
    <name>A0A4W2EVV6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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    <dbReference type="SMR" id="A0A4W2EVV6"/>
    <dbReference type="STRING" id="30522.A0A4W2EVV6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037699.1">
        <property type="protein sequence ID" value="ENSBIXP00000041167.1"/>
        <property type="gene ID" value="ENSBIXG00000005186.1"/>
    </dbReference>
    <dbReference type="OMA" id="CDESGKH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="zinc finger protein 454 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="12"/>
    </dbReference>
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        <property type="entry name" value="KRAB"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="PANTHER" id="PTHR24388:SF96">
        <property type="entry name" value="GENE, 32687-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01352">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00096">
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        <property type="match status" value="12"/>
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    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="333"/>
            <end position="360"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="417"/>
            <end position="444"/>
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    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="445"/>
            <end position="472"/>
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    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="473"/>
            <end position="500"/>
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    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="501"/>
            <end position="527"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="527" mass="60482" checksum="506339F777693910" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYD1</accession>
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        <submittedName>
            <fullName evidence="8">Solute carrier family 23 member 1</fullName>
        </submittedName>
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        <name evidence="8" type="primary">SLC23A1</name>
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    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
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        <property type="nucleotide sequence ID" value="XM_027546322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EYD1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051196.1">
        <property type="protein sequence ID" value="ENSBIXP00000042063.1"/>
        <property type="gene ID" value="ENSBIXG00000005430.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NCS2"/>
    </dbReference>
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        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="428"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="603" mass="65421" checksum="3312CB47E766FCC2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYJ1</accession>
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        <recommendedName>
            <fullName evidence="3">Cysteine-rich and transmembrane domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CYSTM1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYSTM1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EYJ1"/>
    <dbReference type="STRING" id="30522.A0A4W2EYJ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039754.1">
        <property type="protein sequence ID" value="ENSBIXP00000042128.1"/>
        <property type="gene ID" value="ENSBIXG00000026239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016830.1">
        <property type="protein sequence ID" value="ENSBIXP00005009042.1"/>
        <property type="gene ID" value="ENSBIXG00005007492.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000045016"/>
    <dbReference type="OMA" id="ETCLTAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043240">
        <property type="entry name" value="CYSTM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47564">
        <property type="entry name" value="CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47564:SF1">
        <property type="entry name" value="CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="104" mass="11044" checksum="25C5210A1F2D0E3C" modified="2019-09-18" version="1">MNQGNPPPYPGPGPTAPYPPYPSQPMGPGFYPPGPPGGPYPPPQGGYPYQGYPQYGWQGGPQEPPKTTAYVVERERKTEDTGCQAALAACWAALCCCCLLESLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0D0</accession>
    <name>A0A4W2F0D0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0D0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040952.1">
        <property type="protein sequence ID" value="ENSBIXP00000042812.1"/>
        <property type="gene ID" value="ENSBIXG00000006149.1"/>
    </dbReference>
    <dbReference type="OMA" id="EVWCWRS"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="194"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="399" mass="45573" checksum="9DF87BFAD2E18224" modified="2019-09-18" version="1">MMLLKCCTQYASKFGKLSSGHRTGKGQFFIPIPKKGNAKECSNYHTTALILHTSKVMLKILQARLQQYVNRELADVQAGFRKGRGTRDQIANIRWIVEKAREFQKNIYFCFIDYAKAFDCVAHNKLWKILKEMGIPDHLICLLRNVYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAVLEEAQAGIKIARRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKVVASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDGIFKSRDSTLPTKVRLVKAMVFPVVMYGCESWTVKKAERRRIDAFEVWCWRSLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0J5</accession>
    <name>A0A4W2F0J5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 26 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC26A2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Sulfate transporter which mediates sulfate uptake into chondrocytes in order to maintain adequate sulfation of proteoglycans which is needed for cartilage development. Mediates electroneutral anion exchange of sulfate ions for oxalate ions, sulfate and oxalate ions for chloride and/or hydroxyl ions and chloride ions for bromide, iodide and nitrate ions. The coupling of sulfate transport to both hydroxyl and chloride ions likely serves to ensure transport at both acidic pH when most sulfate uptake is mediated by sulfate-hydroxide exchange and alkaline pH when most sulfate uptake is mediated by sulfate-chloride exchange. Essential for chondrocyte proliferation, differentiation and cell size expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>bromide(in) + chloride(out) = bromide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75335"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>iodide(in) + chloride(out) = iodide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72379"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nitrate(in) + chloride(out) = nitrate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75339"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:72275"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>oxalate(out) + 2 chloride(in) = oxalate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75095"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(out) + 2 chloride(in) = sulfate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75091"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0J5"/>
    <dbReference type="SMR" id="A0A4W2F0J5"/>
    <dbReference type="STRING" id="30522.A0A4W2F0J5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041063.1">
        <property type="protein sequence ID" value="ENSBIXP00000042878.1"/>
        <property type="gene ID" value="ENSBIXG00000026876.1"/>
    </dbReference>
    <dbReference type="OMA" id="PALYWIP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000015">
        <property type="entry name" value="Sulfate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="456"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="21">
        <location>
            <begin position="563"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="734" mass="81540" checksum="1657DBE3E8BBDA6B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1H1</accession>
    <name>A0A4W2F1H1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DnaJ homolog subfamily C member 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DNAJC6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Forms a complex composed of HSPA8, CLTC and DNAJC6. Interacts with HSPA8/HSC70 in an ATP-dependent manner; this interaction stimulates the HSPA8's ATPase activity. Interacts with CLTC; this interaction produces a local change in heavy-chain contacts, creating a detectable global distortion of the clathrin coat. Interacts with AP2A2. Interacts with DNM1(GTP-bound form); this interaction allows clathrin-coated vesicle (CCV) formation at the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F1H1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041707.1">
        <property type="protein sequence ID" value="ENSBIXP00000043218.1"/>
        <property type="gene ID" value="ENSBIXG00000027280.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14563">
        <property type="entry name" value="PTP_auxilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000001">
        <property type="entry name" value="cyclin-G-associated kinase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000255">
        <property type="entry name" value="putative tyrosine-protein phosphatase auxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000002">
        <property type="entry name" value="putative tyrosine-protein phosphatase auxilin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE AUXILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="15">
        <location>
            <begin position="55"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="16">
        <location>
            <begin position="228"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="14">
        <location>
            <begin position="849"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="451"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="550"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="599"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="735"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069335"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="913" mass="99924" checksum="8CCEB65EA6D43087" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F289</accession>
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        <submittedName>
            <fullName evidence="4">GIPC PDZ domain containing family member 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">GIPC1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the GIPC family.</text>
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        <property type="component" value="Chromosome 7"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098761">
        <property type="term" value="P:cellular response to interleukin-7"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014047">
        <property type="term" value="P:glutamate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032467">
        <property type="term" value="P:positive regulation of cytokinesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048023">
        <property type="term" value="P:positive regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030511">
        <property type="term" value="P:positive regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099171">
        <property type="term" value="P:presynaptic modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21180">
        <property type="entry name" value="GH2_GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23077">
        <property type="entry name" value="PDZ_GIPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000097">
        <property type="entry name" value="PDZ domain-containing protein GIPC1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055349">
        <property type="entry name" value="GH2_GIPC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056814">
        <property type="entry name" value="GIPC1-3_GH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017379">
        <property type="entry name" value="GIPC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259:SF4">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN GIPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259">
        <property type="entry name" value="RGS-GAIP INTERACTING PROTEIN GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25083">
        <property type="entry name" value="GIPC1_GH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25082">
        <property type="entry name" value="GIPC1_GH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038083">
        <property type="entry name" value="UCP038083_GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="133"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="333" mass="35965" checksum="3D70C0D2350EA608" modified="2019-09-18" version="1">MPLGLGRRKKAPPLVENEEAEPGRGGLGVGEPGPLGGGGAGGPQMGLPPPPPALRPRLVFHTQLAHGSPTGRIEGFTNVKELYGKIAEAFRLPAAEVMFCTLNTHKVDMDKLLGGQIGLEDFIFAHVKGQRKEVEVFKSEEALGLTITDNGAGYAFIKRIKEGSVIDHIQLISVGDMIEAINGQSLLGCRHYEVARLLKELPRGRTFTLKLTEPRKAFDMISVRSGGGRPGSGPQLGTGRGTLRLRSRGPATVEDLPSAFEEKAIEKVDDLLESYMGIRDTELAATMVELGKDKRNPDELAEALDERLGDFAFPDEFVFDVWGAIGDAKVGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F9W1</accession>
    <name>A0A4W2F9W1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="6 14">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GNPNAT1</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="6 14"/>
    <dbReference type="RefSeq" id="XP_027408767.1">
        <property type="nucleotide sequence ID" value="XM_027552966.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027408768.1">
        <property type="nucleotide sequence ID" value="XM_027552967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F9W1"/>
    <dbReference type="SMR" id="A0A4W2F9W1"/>
    <dbReference type="STRING" id="30522.A0A4W2F9W1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049793.1">
        <property type="protein sequence ID" value="ENSBIXP00000021122.1"/>
        <property type="gene ID" value="ENSBIXG00000004885.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012832.1">
        <property type="protein sequence ID" value="ENSBIXP00005002153.1"/>
        <property type="gene ID" value="ENSBIXG00005008547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899728"/>
    <dbReference type="CTD" id="64841"/>
    <dbReference type="GeneTree" id="ENSGT00390000008666"/>
    <dbReference type="OMA" id="LVVEMKF"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000031">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="39"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20776" checksum="19F3181ECF6AA9E2" modified="2019-09-18" version="1">MKPDETPMFDPSLLKEVDWSQNTATFSPAISPTHPGEGLVLRPLCTADLNRGFFKVLGQLTETGVVNPEQFMKSFEHMKKSGDYYVTVVEDVTLGQIVATATLIIEHKFIHSCAKRGRVEDVVVSDECRGKQLGKLLLSTLTLLSKKLNCYKITLECLPQNVGFYKKFGYTVSEENYMCRRFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FBY7</accession>
    <name>A0A4W2FBY7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement component C9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">C9</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Constitutes the pore-forming subunit of the MAC complex: during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homooligomer; about 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375949.1">
        <property type="nucleotide sequence ID" value="XM_027520148.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027375950.1">
        <property type="nucleotide sequence ID" value="XM_027520149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FBY7"/>
    <dbReference type="SMR" id="A0A4W2FBY7"/>
    <dbReference type="STRING" id="30522.A0A4W2FBY7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019013.1">
        <property type="protein sequence ID" value="ENSBIXP00000033092.1"/>
        <property type="gene ID" value="ENSBIXG00000015784.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011507.1">
        <property type="protein sequence ID" value="ENSBIXP00005002810.1"/>
        <property type="gene ID" value="ENSBIXG00005009100.1"/>
    </dbReference>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="548" mass="61998" checksum="69402AB0B20237F9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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        </recommendedName>
    </protein>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="GeneTree" id="ENSGT00390000017536"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="T-cell surface glycoprotein CD5"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SRCR"/>
    </dbReference>
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        <property type="entry name" value="SRCR-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003566">
        <property type="entry name" value="Tcell_CD5"/>
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        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD5"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="SPERACTRCPTR"/>
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
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        <location>
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            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611334" description="T-cell surface glycoprotein CD5" evidence="16">
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            <end position="495"/>
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    </feature>
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            <end position="402"/>
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    </feature>
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        <location>
            <begin position="34"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="17">
        <location>
            <begin position="160"/>
            <end position="266"/>
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    </feature>
    <feature type="domain" description="SRCR" evidence="17">
        <location>
            <begin position="274"/>
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    </feature>
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        <location>
            <begin position="475"/>
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        </location>
    </feature>
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        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
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        <location>
            <begin position="108"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="495" mass="54367" checksum="8D72B01293132AE1" modified="2019-09-18" version="1">MGSQHLPLAALYLLELLVTSCLGGLKVEVQGLTMRLSGSGSRCQGRLEVSNGTEWYAVHSQSWGQLSLYQVAPRQFLKLCQELQCRDPLLLSSSRYFKEVQFQKLIICHGQLGSFSNCSLNRGRQVDSLALICLEPPRTTAPPTTSPPTTTPEPTAPPRFQLVAEPGGLRCAGVVEFYSGGLGGTIGIEPQNDIKDLGQLICAALQCGSFLKPLPETEEAQTQKPEGQRPLPIRWEIQNPKCTSLEQCFRKVQPWVGGQALGLICSDFQPKVQSRLVGGSDVCEGSVEVRSGKGQKWDTLCDDSWAKGTARWEEVCREQQCGNVSSYRGLDPSEKTLGGFYCPPGILSRCHKLEEKKSHCKRVFVTCQNSSRAGLGAGAVMSIILALLLLAVLLVVCGPLAYKKVVKKFRQKKQRQWIGPTGMNQNMSFHRNHTVTVRSQVENATASHVENEYSQPPRNSQISAYPALEGALHRISTQPDNSSDSDYELHGAQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FGU0</accession>
    <name>A0A4W2FGU0_BOBOX</name>
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        <submittedName>
            <fullName evidence="1">Mesenteric estrogen dependent adiposis</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="primary">MEDAG</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_027557481.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2FGU0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000005.1">
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        <property type="gene ID" value="ENSBIXG00005010602.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000018451"/>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MEDAG/TEX26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33769:SF3">
        <property type="entry name" value="MESENTERIC ESTROGEN-DEPENDENT ADIPOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 26 ISOFORM X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="303" mass="34360" checksum="62F572F31081CFFA" modified="2019-09-18" version="1">MAGLESAPAARPSLTSISSGELRSLWTCDCELALLPLGQLLRLQPGAFQLRGDQLVVPAPAEPASARGGFNVFGDGFVRLDGQLYRLSSYMRRYVELTNYCDYKDYRETILSKPMLFFVHVQTKKDSLKERTYAFLVNTRHPKIRRQIEQGMDMVISSVIGESYRLQFDFQEVVKNFFPPGNKVVNGEDLSFAYEFKADALFDFFYWFGLSNSTVKVNGKVLNLSSTSPEKKETIKLFLEKMSEPLIRRSSFSDRKFSVTSRGSIDEVFNCNLSPRSSLMEPLVAELPFPCVLESEETPNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FPX5</accession>
    <name>A0A4W2FPX5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adipogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ADIG</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_027558153.1"/>
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        <property type="gene ID" value="ENSBIXG00000022001.1"/>
    </dbReference>
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        <property type="gene ID" value="ENSBIXG00005012712.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000018723"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:lipid droplet"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:brown fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045600">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050872">
        <property type="term" value="P:white fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR38499">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38499:SF1">
        <property type="entry name" value="ADIPOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15202">
        <property type="entry name" value="Adipogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="81" mass="9696" checksum="0E7378CFC69B4C5A" modified="2019-09-18" version="1">MKYPLVPLVNELTFSFLVFWLCLPVALLLFLLIIWLRFLLSQDSEENDSDVCLDWEPWSKNPDEFCQEEMLHSQEEERPCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FZ58</accession>
    <name>A0A4W2FZ58_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Leucine rich repeat containing 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LRRC4B</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027515029.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027515030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FZ58"/>
    <dbReference type="STRING" id="30522.A0A4W2FZ58"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024296.1">
        <property type="protein sequence ID" value="ENSBIXP00000013737.1"/>
        <property type="gene ID" value="ENSBIXG00000018607.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045357.1">
        <property type="protein sequence ID" value="ENSBIXP00005010813.1"/>
        <property type="gene ID" value="ENSBIXG00005015365.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876150"/>
    <dbReference type="CTD" id="94030"/>
    <dbReference type="GeneTree" id="ENSGT00940000160261"/>
    <dbReference type="OMA" id="YKAHYNN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044300">
        <property type="term" value="C:cerebellar mossy fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099151">
        <property type="term" value="P:regulation of postsynaptic density assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905606">
        <property type="term" value="P:regulation of presynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000012">
        <property type="entry name" value="Leucine rich repeat containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611486" evidence="16">
        <location>
            <begin position="36"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="364"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="464"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="488"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="534"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="715" mass="76547" checksum="86C69FEC98DF1B13" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FZN9</accession>
    <name>A0A4W2FZN9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type natriuretic peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NPPC</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Acts by specifically binding and stimulating NPR2 to produce cGMP. Binds the clearance receptor NPR3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the natriuretic peptide family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FZN9"/>
    <dbReference type="STRING" id="30522.A0A4W2FZN9"/>
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        <property type="protein sequence ID" value="ENSBIXP00000036627.1"/>
        <property type="gene ID" value="ENSBIXG00000000474.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019546.1">
        <property type="protein sequence ID" value="ENSBIXP00005010923.1"/>
        <property type="gene ID" value="ENSBIXG00005015477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015492"/>
    <dbReference type="OMA" id="HDYPNAR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006182">
        <property type="term" value="P:cGMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002406">
        <property type="entry name" value="C_natriurtcpep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000663">
        <property type="entry name" value="Natr_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030480">
        <property type="entry name" value="Natr_peptide_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12167">
        <property type="entry name" value="C-TYPE NATRIURETIC PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12167:SF2">
        <property type="entry name" value="C-TYPE NATRIURETIC PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00212">
        <property type="entry name" value="ANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00713">
        <property type="entry name" value="CNATPEPTIDE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00710">
        <property type="entry name" value="NATPEPTIDES"/>
    </dbReference>
    <dbReference type="SMART" id="SM00183">
        <property type="entry name" value="NAT_PEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00263">
        <property type="entry name" value="NATRIURETIC_PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="7 9" id="KW-0838">Vasoactive</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="88"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="193" mass="20294" checksum="6AF2AB1B8DB96D7B" modified="2019-09-18" version="1">MTSAAGWIIKARAEPRTQSAPSRRRAALGPASASQPGQPAPRSPACRLARPAHPRCRSSAPRHPTGTMHLSQLLACALLLALLSLRPSEAKPGAPPKVPRTPSGEEVAEPQAAGGGQKKGDKTPGGGGANLKDDRSRLLRDLRVDTKSRAAWTRLLHEHPNARKYKGGNKKGLSKGCFGLKLDRIGSMSGLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G1E9</accession>
    <name>A0A4W2G1E9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transmembrane protease serine 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Matriptase-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TMPRSS7</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Serine protease which preferentially hydrolyzes peptides with Arg at the P1 position.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterodimer with SERPINA5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2G1E9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030247.1">
        <property type="protein sequence ID" value="ENSBIXP00000017630.1"/>
        <property type="gene ID" value="ENSBIXG00000021612.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019133.1">
        <property type="protein sequence ID" value="ENSBIXP00005010653.1"/>
        <property type="gene ID" value="ENSBIXG00005015268.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160085"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000033">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
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    <sequence length="779" mass="86752" checksum="033E0C748A3ECF50" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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            <authorList>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIG0</accession>
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        <recommendedName>
            <fullName evidence="4">Sorting nexin-2</fullName>
        </recommendedName>
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        <name evidence="17" type="primary">SNX2</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Required for retrograde endosome-to-TGN transport of TGN38. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027546972.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000020821.1"/>
        <property type="gene ID" value="ENSBIXG00000004821.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029907.1">
        <property type="protein sequence ID" value="ENSBIXP00005017840.1"/>
        <property type="gene ID" value="ENSBIXG00005021360.1"/>
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    <dbReference type="GeneID" id="113895589"/>
    <dbReference type="CTD" id="6643"/>
    <dbReference type="GeneTree" id="ENSGT00940000155798"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030905">
        <property type="term" value="C:retromer, tubulation complex"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005154">
        <property type="term" value="F:epidermal growth factor receptor binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:1990460">
        <property type="term" value="F:leptin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:transferrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:early endosome to Golgi transport"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:lamellipodium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="PX_SNX2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Sorting nexin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
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        <property type="entry name" value="SNX2_PX"/>
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    <dbReference type="InterPro" id="IPR005329">
        <property type="entry name" value="Sorting_nexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
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    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF31">
        <property type="entry name" value="SORTING NEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03700">
        <property type="entry name" value="Sorting_nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="16">
        <location>
            <begin position="140"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="30"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="93"/>
            <end position="104"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00020435"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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            <dbReference type="ARBA" id="ARBA00045620"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017840.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="523" mass="58935" checksum="0B2A2EBECD4C84B7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GSC4</accession>
    <name>A0A4W2GSC4_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Undifferentiated embryonic cell transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UTF1</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="A0A4W2GSC4"/>
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        <property type="gene ID" value="ENSBIXG00005024961.1"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="project" value="Ensembl"/>
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            <end position="127"/>
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            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="343" mass="36680" checksum="D4B23FF6D1297849" modified="2019-09-18" version="1">MLLRPRRPPPPEPPSPASPDPEPRLAGGAPETPPRRPASPGSPASPGSPVSPGSAQRTPWSAHETELLLGTLLQPAVWRALLLDRRQALPTYRRVSAALARQQVRRTPAQCRRRYKFLKDKVRDAHGQPPGPFDAQIRQLMGLLGDNGRRRGRRRSPGPGRPPRGRRPGGARAPGNRRLRVPGAPPLPASRELDSDPAWTLRFSPSPPKSADAPHAPGSPTAPSTFTPAPVRPEEPESFRAPGSPPPPSPGPAREDPDSPPARLEDHAPQQPAPPSLNAALLQTLGHLGDIVSILGPLRDQLLTLNQHVEQLRGSFDQTVSLAVGFILGSAAAERGVLADPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H5D1</accession>
    <name>A0A4W2H5D1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lipid transferase CIDEB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cell death-inducing DFFA-like effector B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CIDEB</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with DFFA. Interacts with DFFB; inhibited by DFFB. Interacts with APOB. Interacts with PREB/SEC12; facilitating loading of SCAP-SREBP into COPII vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CIDE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027409003.1">
        <property type="nucleotide sequence ID" value="XM_027553202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H5D1"/>
    <dbReference type="SMR" id="A0A4W2H5D1"/>
    <dbReference type="STRING" id="30522.A0A4W2H5D1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033926.1">
        <property type="protein sequence ID" value="ENSBIXP00000039519.1"/>
        <property type="gene ID" value="ENSBIXG00000023446.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041907.1">
        <property type="protein sequence ID" value="ENSBIXP00005025942.1"/>
        <property type="gene ID" value="ENSBIXG00005028374.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899859"/>
    <dbReference type="CTD" id="27141"/>
    <dbReference type="GeneTree" id="ENSGT00390000018596"/>
    <dbReference type="OMA" id="PFRICCN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030137">
        <property type="term" value="C:COPI-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120013">
        <property type="term" value="F:lipid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097194">
        <property type="term" value="P:execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160077">
        <property type="term" value="P:lipid droplet fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090207">
        <property type="term" value="P:regulation of triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034379">
        <property type="term" value="P:very-low-density lipoprotein particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000005">
        <property type="entry name" value="Cell death activator CIDE-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306">
        <property type="entry name" value="CELL DEATH ACTIVATOR CIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306:SF10">
        <property type="entry name" value="LIPID TRANSFERASE CIDEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02017">
        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00266">
        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51135">
        <property type="entry name" value="CIDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CIDE-N" evidence="17">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="219" mass="24650" checksum="05928C8ADDD7D1BC" modified="2019-09-18" version="1">MEYLSNLDPSSLLRSVSNMSADLGRKVWTSAPPRQRPFRVCDNKRTTRKGLTAATRQELLDKALEALVLSGALTLVLEEDGTTVESEEFFQLLEDDTCLMVLELGQSWSPRRSGVLSYGLGQEKPKHSKDIARITFDVYKQSPRDLFGSLNIKATFYGLYSLSCDIQGLGPKKILRELLRWASSLLQGLGHMLLGISSTLRRAVEGTERWQRQGRLKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H7V6</accession>
    <name>A0A4W2H7V6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Aminopeptidase</fullName>
            <ecNumber evidence="23">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ERAP1</name>
    </gene>
    <organism evidence="28 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 23">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. May also exist as a heterodimer; with ERAP2. Interacts with RBMX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="23"/>
    <dbReference type="RefSeq" id="XP_027401349.1">
        <property type="nucleotide sequence ID" value="XM_027545548.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401350.1">
        <property type="nucleotide sequence ID" value="XM_027545549.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401351.1">
        <property type="nucleotide sequence ID" value="XM_027545550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H7V6"/>
    <dbReference type="SMR" id="A0A4W2H7V6"/>
    <dbReference type="STRING" id="30522.A0A4W2H7V6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031686.1">
        <property type="protein sequence ID" value="ENSBIXP00000018565.1"/>
        <property type="gene ID" value="ENSBIXG00000002814.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055441.1">
        <property type="protein sequence ID" value="ENSBIXP00005027204.1"/>
        <property type="gene ID" value="ENSBIXG00005030891.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894981"/>
    <dbReference type="CTD" id="51752"/>
    <dbReference type="GeneTree" id="ENSGT00940000159086"/>
    <dbReference type="OMA" id="NGVCIRN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005138">
        <property type="term" value="F:interleukin-6 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002502">
        <property type="term" value="P:peptide antigen assembly with MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000007">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000003">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF156">
        <property type="entry name" value="ENDOPLASMIC RETICULUM AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1064">Adaptive immunity</keyword>
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        <location>
            <begin position="1"/>
            <end position="32"/>
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            <end position="942"/>
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            <end position="247"/>
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            <begin position="282"/>
            <end position="486"/>
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            <dbReference type="ARBA" id="ARBA00004648"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
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    <sequence length="942" mass="107064" checksum="2DFC7FE960BC8149" modified="2019-09-18" version="1"> </sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="C:presynaptic active zone membrane"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031630">
        <property type="term" value="P:regulation of synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22809">
        <property type="entry name" value="Complexin_NTD_CPLX_III_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.580">
        <property type="entry name" value="Single Helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF5">
        <property type="entry name" value="COMPLEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="158" mass="17616" checksum="CBBF904A6DB6EA0F" modified="2019-09-18" version="1">MAFMVKTMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERATLRSHFRDKYRLPKNETDESQIQMAGGDVELPRELAKMIEEDTEEEEERASVLGQLASLPGLDLGSLKDKAQSTLGDLKQSAEKCHIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HN18</accession>
    <name>A0A4W2HN18_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HN18"/>
    <dbReference type="SMR" id="A0A4W2HN18"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000083.1">
        <property type="protein sequence ID" value="ENSBIXP00000000586.1"/>
        <property type="gene ID" value="ENSBIXG00000029636.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005396.1">
        <property type="protein sequence ID" value="ENSBIXP00005031224.1"/>
        <property type="gene ID" value="ENSBIXG00005011496.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244868"/>
    <dbReference type="OMA" id="WHTISIA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002448">
        <property type="entry name" value="OBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF89">
        <property type="entry name" value="ALLERGEN BOS D 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01173">
        <property type="entry name" value="ODORANTBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611943" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="6">
        <location>
            <begin position="16"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="7">
        <location>
            <begin position="29"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="172" mass="19297" checksum="971FBED503E0A7BF" modified="2019-09-18" version="1">MKAVFLTLLLGLVCAAQEAPAQPESPEITGNWYTIYMAANNKEKIEEGGPLRTYFRQFECIDNCEKMSITFIVTHYDSCTLLTVVAQRAEGNVYHVDFMGKNSVQLIPVSESMLVFYAENFDGEKTTKLTYALGKGDSLSQEDIQKYEEINNERGIPNENTEDGAQTDNCPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HVC4</accession>
    <name>A0A4W2HVC4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Voltage-gated potassium channel subunit beta-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">K(+) channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Kv-beta-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCNAB2</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027420891.1">
        <property type="nucleotide sequence ID" value="XM_027565090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HVC4"/>
    <dbReference type="SMR" id="A0A4W2HVC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025350.1">
        <property type="protein sequence ID" value="ENSBIXP00000014453.1"/>
        <property type="gene ID" value="ENSBIXG00000019149.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021691.1">
        <property type="protein sequence ID" value="ENSBIXP00005035456.1"/>
        <property type="gene ID" value="ENSBIXG00005016637.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906651"/>
    <dbReference type="CTD" id="8514"/>
    <dbReference type="GeneTree" id="ENSGT00940000157867"/>
    <dbReference type="OMA" id="YLPWSPL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098900">
        <property type="term" value="P:regulation of action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19141">
        <property type="entry name" value="Aldo_ket_red_shaker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000001">
        <property type="entry name" value="voltage-gated potassium channel subunit beta-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005401">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF1">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01579">
        <property type="entry name" value="KCNAB2CHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="22">
        <location>
            <begin position="52"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="367" mass="40985" checksum="2C8B5EDED031AED8" modified="2019-09-18" version="1">MYPESTTGSPARLSLRQTGSPGMIYSTRYGSPKRQLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEQLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASSADQLMENIGAIQVLPKLSSSIIHEIDSILGNKPYSKKDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IRM4</accession>
    <name>A0A4W2IRM4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cyclin Y like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CCNYL1</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin Y subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027421557.1">
        <property type="nucleotide sequence ID" value="XM_027565756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IRM4"/>
    <dbReference type="SMR" id="A0A4W2IRM4"/>
    <dbReference type="STRING" id="30522.A0A4W2IRM4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023282.1">
        <property type="protein sequence ID" value="ENSBIXP00000013052.1"/>
        <property type="gene ID" value="ENSBIXG00000007389.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056926.1">
        <property type="protein sequence ID" value="ENSBIXP00005047044.1"/>
        <property type="gene ID" value="ENSBIXG00005031627.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906938"/>
    <dbReference type="CTD" id="151195"/>
    <dbReference type="GeneTree" id="ENSGT00940000154453"/>
    <dbReference type="OMA" id="RWADAYQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20540">
        <property type="entry name" value="CYCLIN_CCNY_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000011">
        <property type="entry name" value="Cyclin-Y isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012399">
        <property type="entry name" value="Cyclin_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028934">
        <property type="entry name" value="Cyclin_CG14939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="195"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005047044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="363" mass="41128" checksum="3FA0123274855154" modified="2019-09-18" version="1">MGNTLTCCVSPSASPKLGRRAGPAEPDYESEVYEAAAGDAVAVAPAPATVEPTELDFGAGEGHHLQHISDREMPEDLALESNPSDHPRASTIFLSKSQTDVREKRKSNHLNHVSPGQLTKKYSSCSTIFLDDSTVSQPNLRTTIKCVTLAIYYHIKNRDANRSLDIFDERSHPLTREKVPEEYFKHDPEHKFIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPTNWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNEMERHFLELLQFNINVPASVYAKYYFDLRSLADDNNLNFLFAPLSKERAQNLEAISRLCEDKYKDLCRVAVRRSFSADNFIGIQRSNAILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ITX0</accession>
    <name>A0A4W2ITX0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NIF3-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NIF3L1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Interacts with COPS2. Interacts with THOC7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ITX0"/>
    <dbReference type="STRING" id="30522.A0A4W2ITX0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021500.1">
        <property type="protein sequence ID" value="ENSBIXP00000011899.1"/>
        <property type="gene ID" value="ENSBIXG00000000794.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056776.1">
        <property type="protein sequence ID" value="ENSBIXP00005046922.1"/>
        <property type="gene ID" value="ENSBIXG00005031554.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003590"/>
    <dbReference type="OMA" id="KYHEFFD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000004">
        <property type="entry name" value="NIF3-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017222">
        <property type="entry name" value="DUF34/NIF3_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037490">
        <property type="entry name" value="UCP037490_NIF3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="392" mass="43673" checksum="65098897742D96E1" modified="2019-09-18" version="1">MFGFFLVYSVLPDFCMLSSRVRLVATTARLVHSLIYSSSRSFMDLKALLSSLNDFASLSFAESWDNVGLLVEPSPPHTVNTLFLTNDLTEEVMEEALQKKADLILSYHPPIFRPMKRITWKTWKERLVIRALENRVGIYSPHTAYDAAPQGVNNWLAKGLGVCTSRPIHPSKAPDYPTEGTHRVEFSVTHTQDPDKVISALKEIAGVSVTSFSARTDDEEQTRLSLNCTQQALMQVVAFLSQNRQFYQKTEILSLEKPLLLYTGMGRLCTLDESVSLETMIERIKSHLKLSHVRLALGIGKTLESPVKVVALCAGSGSSVLQGTDADLYLTGEMSHHDVLDAASQGISVILCEHSNTERGFLSDLRDMLDAHLENKINIILSETDRDPLHVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BL40</accession>
    <name>A0A4W2BL40_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease P protein subunit p20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP complexes. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5. Interacts with SMN1. POP7 forms a heterodimer with RPP25 that binds to the P3 stem loop of the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BL40"/>
    <dbReference type="STRING" id="30522.A0A4W2BL40"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000059.1">
        <property type="protein sequence ID" value="ENSBIXP00000000564.1"/>
        <property type="gene ID" value="ENSBIXG00000029664.1"/>
    </dbReference>
    <dbReference type="OMA" id="DWREAHS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000002">
        <property type="entry name" value="Ribonuclease P protein subunit p20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014612">
        <property type="entry name" value="Pop7/Rpp20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12328">
        <property type="entry name" value="Rpp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="91"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="414" mass="45603" checksum="46BE370EC0693344" modified="2019-09-18" version="1">MGRRLPVNCRGVSERCPPTRTGLRYSFTRSWSLGDANLGREVGSANSATPPDPRNCDGRRHLPDHVLWNSAPSLPSLEGPDSQILQAPTKVSSPLFSPWGPHPPPRPPPPASHPREPGTWTGRKKPELALPRSSPDSDLVHASRLRAPPKRRTRGRSRQPRKPRVIQTHRAVLPALRKGAGRTQAGKAIRGSLRPGRGRGDGRACALGTLFRRPAPTGLQHSGVCVARPSSLLGSWWSRAAPPPPASRRNARLAWQRSGPRARGKHGDWREAHSMAENRESRGTVEAELDPVEYTLRKRLPHRLPRRPNDIYVNMKTDFKAQLARCQKLLDGGARGQNACSEIYIHGLGLAINRAINIALQLQAGSFGSLQVAANTSTVELVDELEPETDSREPLTRIRNNSAIHIRVFRVTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BLQ3</accession>
    <name>A0A4W2BLQ3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C29H11orf54</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2BLQ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015404.1">
        <property type="protein sequence ID" value="ENSBIXP00000000804.1"/>
        <property type="gene ID" value="ENSBIXG00000029348.1"/>
    </dbReference>
    <dbReference type="OMA" id="RNMGEGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046599">
        <property type="term" value="P:regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553">
        <property type="entry name" value="ALMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553:SF25">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 295 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="668"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="951"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1026"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1292"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1336"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1444"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1996"/>
            <end position="2027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2184"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="266"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="888"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="960"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1307"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1451"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2000"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2187"/>
            <end position="2204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2612" mass="295828" checksum="B6437EE3A5C7164A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMC8</accession>
    <name>A0A4W2BMC8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box/LRR-repeat protein 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">F-box and leucine-rich repeat protein 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FBXL16</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027382893.1">
        <property type="nucleotide sequence ID" value="XM_027527092.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027382894.1">
        <property type="nucleotide sequence ID" value="XM_027527093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMC8"/>
    <dbReference type="SMR" id="A0A4W2BMC8"/>
    <dbReference type="STRING" id="30522.A0A4W2BMC8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014828.1">
        <property type="protein sequence ID" value="ENSBIXP00000001073.1"/>
        <property type="gene ID" value="ENSBIXG00000028990.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040564.1">
        <property type="protein sequence ID" value="ENSBIXP00005043811.1"/>
        <property type="gene ID" value="ENSBIXG00005027587.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883416"/>
    <dbReference type="CTD" id="146330"/>
    <dbReference type="GeneTree" id="ENSGT00940000159138"/>
    <dbReference type="OMA" id="FWAGLMP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22127">
        <property type="entry name" value="F-box_FBXL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000262">
        <property type="entry name" value="F-box/LRR-repeat protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000733">
        <property type="entry name" value="F-box/LRR-repeat protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="10">
        <location>
            <begin position="325"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="482" mass="51967" checksum="0EA144E12FB4D25D" modified="2019-09-18" version="1">MSSPGVNGDPKPPCLPRNGLVKLPGQPNGLGAASITKGTPAAKNRPCQPPPPTLPPPSLAAPPPRAALAGGLCPQAGLPLGGPALAPVSGPPAERPPLATDEKILNGLFWYFSACEKCVLAQVCKAWRRVLYQPKFWAGLTPVLHAKELYTVLPGGEKEFVNLQGFAARGFEGFCLVGVSDLDICEFIDNYALSKKGVKAMSLKRSTITDAGLEVMLEQMQGVVRLELSGCNDFTEAGLWSSLSARITSLSVSDCINVADDAIAAISQLLPNLAELSLQAYHVTDTALAYFTARQGHSTHTLRLLSCWEITNHGVVNVVHSLPNLTALSLSGCSKVTDDGVELVAENLRKLRSLDLSWCPRITDMALEYVACDLHRLEELVLDRCVRITDTGLSYLSTMSSLRSLYLRWCCQVQDFGLKHLLAMRSLRLLSLAGCPLLTATGLSGLVQLQDLEELELTNCPGATPELFKYFSQHLPRCLVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNR5</accession>
    <name>A0A4W2BNR5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ssu-2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">SSUH2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNR5"/>
    <dbReference type="STRING" id="30522.A0A4W2BNR5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013623.1">
        <property type="protein sequence ID" value="ENSBIXP00000001611.1"/>
        <property type="gene ID" value="ENSBIXG00000000041.1"/>
    </dbReference>
    <dbReference type="OMA" id="KRCNTCS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052789">
        <property type="entry name" value="SSUH2_homolog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48465">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48465:SF1">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="420" mass="47024" checksum="271A51F1FDA83A8F" modified="2019-09-18" version="1">MFPSVVFKSGNNGPAVLGAAEGGPSDPSGLGPLGGQTLPRLDIWTMDRDLSDDDSVVDLSFEAESPLAPPAELLETLPSCDWVLQGDRHQIFFPPSETAGRPQEQRCWSSFLEHRVPVVTEEVAREALLSFVNSKCCYGSAAASDLVILELKQQNLCRYRLETFSESRISEWTFQPFTNHSVDGPQRGTSPRLWDIKVQVPPMFQEDTRRFQVPHSSLVKECHKCHGRGRYKCSGCHGAGTARCSSCSGAKRKAKSARRCQMCSGSGRRRCSTCSGRGNKTCATCKGEKKLLHFIQLVIAWKNSLFEFVSEHQLDFPGELLAKARGETLFKDENTTVYPIVDFPLREISLASRRGISEHSAALASRARVLQQRQTLELIPLTEVRYWYQGKTDVYYIYGTDRQVYLVDYPERYCCGCTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQ29</accession>
    <name>A0A4W2BQ29_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LIAT1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQ29"/>
    <dbReference type="STRING" id="30522.A0A4W2BQ29"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015179.1">
        <property type="protein sequence ID" value="ENSBIXP00000000909.1"/>
        <property type="gene ID" value="ENSBIXG00000029222.1"/>
    </dbReference>
    <dbReference type="OMA" id="SSATCYV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038794">
        <property type="entry name" value="LIAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36474">
        <property type="entry name" value="PROTEIN LIAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36474:SF1">
        <property type="entry name" value="PROTEIN LIAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23298" checksum="004946F3C63AD1B0" modified="2019-09-18" version="1">PRTGQYLGEQCRGELGICVERTRRWESGPRSGPSSATCYVTLERQVSQRLTLPICSADKHQSQGMKTQQLSPTFHDILGPSKDHGPGPEHRQNRDESKLFSYSTTVSLPRFVEIEETLSNRVNESLRWDGILADPEAEKERIRIYKLNRRKRYRIWALKGFHSDPGASETPEDPAYLSDQDSGSSGQLTAEGPSHCSEGNLMSTAIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BT82</accession>
    <name>A0A4W2BT82_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">RFFL</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BT82"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012534.1">
        <property type="protein sequence ID" value="ENSBIXP00000002130.1"/>
        <property type="gene ID" value="ENSBIXG00000008640.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16707">
        <property type="entry name" value="RING-HC_CARP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.140:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049320">
        <property type="entry name" value="CARP1_2_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
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    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RIFIFYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21272">
        <property type="entry name" value="FYVE_CARP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="310"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="166"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00022786"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="357" mass="39870" checksum="52CEF3ADD76CAF7C" modified="2019-09-18" version="1">MWATCCNWFCLDGQPEEASPPQGARTQAYSNPGYSSFPSPTGSEPSCKACGTHFASMARKQQTCLDCKKNFCTSCSSQVGAGPRLCLLCQRFRATDFRREELMKMKVKDLRDYLSLHDISTDMCREKEELVFLVLGQQPVISQEGRTQAPHLGPDFPEQQAFLARPHTSTVPPTSPGLQSAPPGQAQGRQQANGHVSQDQEEPIYLERTARAPAEDETQSVDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVCSAEDQNGGAVPSSLEENLCRICMDSPIDCVLLECGHMVTCTKCGKRMNECPICRQYVIRAVHVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BU51</accession>
    <name>A0A4W2BU51_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BU51"/>
    <dbReference type="SMR" id="A0A4W2BU51"/>
    <dbReference type="STRING" id="30522.A0A4W2BU51"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009462.1">
        <property type="protein sequence ID" value="ENSBIXP00000003634.1"/>
        <property type="gene ID" value="ENSBIXG00000010051.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Uroplakin-2"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021284657" evidence="2">
        <location>
            <begin position="27"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="185" mass="19619" checksum="CD8BA31D57489173" modified="2019-09-18" version="1">MASPWPVWTLSWILILLAVLVPGAAADFNISSLSGLLSPVMTESLLVALPPCHLTGGNATLTVRRANDSKVVRSSFVVPPCRGRRELVSVVDSGSGFTVTRLSAYQVTNLAPGTKYYISYLVTKGASTESSREIPMSTFPRRKAESIGLAMARTGGMVVITVLLSVAMFLLVLGLIIALALGARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUQ1</accession>
    <name>A0A4W2BUQ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">ATP-binding cassette sub-family G member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Sterolin-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>cholesterol(in) + ATP + H2O = cholesterol(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39051"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:39052"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>sitosterol(in) + ATP + H2O = sitosterol(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27693"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:39104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer with ABCG8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUQ1"/>
    <dbReference type="STRING" id="30522.A0A4W2BUQ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008926.1">
        <property type="protein sequence ID" value="ENSBIXP00000003906.1"/>
        <property type="gene ID" value="ENSBIXG00000010296.1"/>
    </dbReference>
    <dbReference type="OMA" id="VFSHIGM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03234">
        <property type="entry name" value="ABCG_White"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001069">
        <property type="entry name" value="ATP-binding cassette, sub-family G (WHITE), member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF113">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="23">
        <location>
            <begin position="50"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00065141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="619" mass="69688" checksum="0A089BBB3B205902" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWN9</accession>
    <name>A0A4W2BWN9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock 70 kDa protein 12A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HSPA12A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with SORL1 (via cytosolic C-terminus); this interaction affects SORL1 internalization and subcellular localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384216.1">
        <property type="nucleotide sequence ID" value="XM_027528415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BWN9"/>
    <dbReference type="STRING" id="30522.A0A4W2BWN9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007255.1">
        <property type="protein sequence ID" value="ENSBIXP00000004708.1"/>
        <property type="gene ID" value="ENSBIXG00000000754.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026299.1">
        <property type="protein sequence ID" value="ENSBIXP00005015442.1"/>
        <property type="gene ID" value="ENSBIXG00005019246.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884173"/>
    <dbReference type="CTD" id="259217"/>
    <dbReference type="GeneTree" id="ENSGT00940000154551"/>
    <dbReference type="OMA" id="MGRCTSR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11735">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000061">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000011">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026685">
        <property type="entry name" value="HSPA12A_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF46">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="692" mass="77095" checksum="F3CAE94089DE248B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C0Q1</accession>
    <name>A0A4W2C0Q1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C0Q1"/>
    <dbReference type="STRING" id="30522.A0A4W2C0Q1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004046.1">
        <property type="protein sequence ID" value="ENSBIXP00000006295.1"/>
        <property type="gene ID" value="ENSBIXG00000012408.1"/>
    </dbReference>
    <dbReference type="OMA" id="GVCNHKN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051044">
        <property type="term" value="P:positive regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF20">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021309372" evidence="6">
        <location>
            <begin position="23"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="8">
        <location>
            <begin position="197"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="7">
        <location>
            <begin position="405"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="561"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="646"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="699"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="723"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="772" mass="82063" checksum="3F9197B59A0F7406" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C183</accession>
    <name>A0A4W2C183_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tektin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TEKT5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules. Required for normal sperm mobility.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Sperm-specific microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in flagellar axoneme. Forms an extensive interaction network in different conformations that reinforces the helix bundle composed by other tektin proteins (TEKT1 to TEKT4) and MIPs to anchor the tektin bundle onto the tubulin wall of A-tubule of the sperm flagellum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with TEKT3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytoskeleton</location>
            <location evidence="12">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C183"/>
    <dbReference type="STRING" id="30522.A0A4W2C183"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053980.1">
        <property type="protein sequence ID" value="ENSBIXP00000000022.1"/>
        <property type="gene ID" value="ENSBIXG00000030358.1"/>
    </dbReference>
    <dbReference type="OMA" id="IANVQTC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF23">
        <property type="entry name" value="TEKTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 12" id="KW-0969">Cilium</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="489" mass="56490" checksum="5B9A2871129C72C9" modified="2019-09-18" version="1">MEFLGTTQTATYCGPKKPCGGSVLPSVASGPTVQECYQPYYLPGYRYLNAWRPSLFYKVANAQTCLDEGATCRSALRPPTILPAVRSALFCRYSPQDWDQSNRLQLCGAEASRLWAGRMTGDSMRLMQDKDQLTRQMQEGTCRNLGQRLSDIGFWKSELNYELERLLNENHSLDAIKRRLECAAEELRCPLQVALECLYQREKRIGIDLVHDNVEKNLIREVDMIKCCQEQMRKLAQRIDIQMRDNRDAQHALERDLEDKNSAEFIDEKCLNLRNTSDCISFFHGVEKIDGTISVPETWAKFSNDNIRHSQNMRANSIRLREEAQNLFDVLADQLWRQFTNTNLAFNARIAEETDVKNKLQTQLAKTLQEIFQTENTIMLLERSIMAKECPLKVAQTRLECRTRRPNVELCRDSPQFKLVNEVFTIDDTLQTLKLRLRETRDTLQLLVMTKCRLEHELAIKANTLCIDKEKCMAMRKTFPSTPCMGICP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C246</accession>
    <name>A0A4W2C246_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEPTIN3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Filament-forming cytoskeletal GTPase. May play a role in cytokinesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C246"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004996.1">
        <property type="protein sequence ID" value="ENSBIXP00000005824.1"/>
        <property type="gene ID" value="ENSBIXG00000011963.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015580.1">
        <property type="protein sequence ID" value="ENSBIXP00005000733.1"/>
        <property type="gene ID" value="ENSBIXG00005029864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158004"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000387">
        <property type="entry name" value="neuronal-specific septin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008114">
        <property type="entry name" value="Septin3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01741">
        <property type="entry name" value="SEPTIN3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="11">
        <location>
            <begin position="58"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="358" mass="40666" checksum="31453DF1365D6107" modified="2019-09-18" version="1">MSKGLPETRTDAAMSELVPEPRPKPAVPMKPIGINPNLLGYIGIDTIIEQMRKKTMKTGFDFNIMVVGQSGLGKSTLVNTLFKSQVSRKASSWNREEKIPKTVEIKAIGHVIEEGGVKMKLTVIDTPGFGDQINNENCWEPIEKYINEQYEKFLKEEVNIARKKRIPDTRVHCCLYFISPTGHSLRPLDLEFMKHLSKVVNIIPVIAKADTMTLEEKSEFKQRVRKELEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRVLGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGGLPPGEGLLGTVLPPVPATPCPTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3P0</accession>
    <name>A0A4W2C3P0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kelch repeat and BTB domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KBTBD8</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification. The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the BCR(KBTBD8) E3 ubiquitin ligase complex, at least composed of CUL3, KBTBD8 and RBX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KBTBD8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027378761.1">
        <property type="nucleotide sequence ID" value="XM_027522960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3P0"/>
    <dbReference type="SMR" id="A0A4W2C3P0"/>
    <dbReference type="STRING" id="30522.A0A4W2C3P0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015155.1">
        <property type="protein sequence ID" value="ENSBIXP00000000918.1"/>
        <property type="gene ID" value="ENSBIXG00000029203.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056132.1">
        <property type="protein sequence ID" value="ENSBIXP00005046389.1"/>
        <property type="gene ID" value="ENSBIXG00005031227.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880596"/>
    <dbReference type="CTD" id="84541"/>
    <dbReference type="GeneTree" id="ENSGT00940000158653"/>
    <dbReference type="OMA" id="FWMYDHA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014029">
        <property type="term" value="P:neural crest formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18274">
        <property type="entry name" value="BTB_POZ_KBTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
        <property type="entry name" value="Kelch repeat and BTB domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000014">
        <property type="entry name" value="kelch repeat and BTB domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000020">
        <property type="entry name" value="Kelch repeat and BTB domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028764">
        <property type="entry name" value="BTB/POZ_KBTBD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF433">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="601" mass="68883" checksum="48C608B7AF767EBB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5D8</accession>
    <name>A0A4W2C5D8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ubiquitin conjugating enzyme E2 D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UBE2D1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384357.1">
        <property type="nucleotide sequence ID" value="XM_027528556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5D8"/>
    <dbReference type="SMR" id="A0A4W2C5D8"/>
    <dbReference type="STRING" id="30522.A0A4W2C5D8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000683.1">
        <property type="protein sequence ID" value="ENSBIXP00000007989.1"/>
        <property type="gene ID" value="ENSBIXG00000013849.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029913.1">
        <property type="protein sequence ID" value="ENSBIXP00005039027.1"/>
        <property type="gene ID" value="ENSBIXG00005005677.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884227"/>
    <dbReference type="CTD" id="7321"/>
    <dbReference type="GeneTree" id="ENSGT00940000155109"/>
    <dbReference type="OMA" id="NFHWQAT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000101">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="147" mass="16602" checksum="2E96FD0179EE119D" modified="2019-09-18" version="1">MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6B2</accession>
    <name>A0A4W2C6B2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6B2"/>
    <dbReference type="SMR" id="A0A4W2C6B2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013145.1">
        <property type="protein sequence ID" value="ENSBIXP00000001823.1"/>
        <property type="gene ID" value="ENSBIXG00000000072.1"/>
    </dbReference>
    <dbReference type="OMA" id="PRIRWRD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="99"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="500" mass="57890" checksum="C7638D6E77CAF2FF" modified="2019-09-18" version="1">MQKMGSIKDRNGMDLTEPEDIKKRWQEYTEELYKKELHNRDNHDGVITDLETDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIGKTQQWSQDWKRSVFIPIPKKGNAKECSNYRTIILISHDSKIMLKILQARLQQYVNRELPDVQAGFRKGRGTRDRIANICWIMEKAREFQKNIYFCFIDHAKAFDCVDHNKLWKILKEMGIPDHLTCLFRNLYADQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEGELKSLLMKVKEESEKVGLNLNIQNTKIMASGPITSWQIDGETVSDFMVFPVVMYGCESWIIKKAERQRIDAFELWCWKRFLRVPWTARRSNQYILKKISPGCSLEGLMLKLKLQYFGHFMQRVDSLEKTLMLGGIGGRRRRGRQRMRWLDAITDSMDVSLSELWELVMCREAWRAAIHGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6L2</accession>
    <name>A0A4W2C6L2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP complex mu/sigma subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC113880014</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377777.1">
        <property type="nucleotide sequence ID" value="XM_027521976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6L2"/>
    <dbReference type="SMR" id="A0A4W2C6L2"/>
    <dbReference type="STRING" id="30522.A0A4W2C6L2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016118.1">
        <property type="protein sequence ID" value="ENSBIXP00000008441.1"/>
        <property type="gene ID" value="ENSBIXG00000014281.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880014"/>
    <dbReference type="OMA" id="MNDQGKP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14834">
        <property type="entry name" value="AP3_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000001">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027155">
        <property type="entry name" value="APS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="193" mass="22035" checksum="D219EF4A9FFED6EA" modified="2019-09-18" version="1">MIQAILVFNNHGKPRLVRFYQRFPEEIQQQIVRETFHLVLKRDDNICNFLEGGSLIGGSDYKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHMDKVHYILQEVVMGGMVLETNMNEIVAQIEAQNRLEKSEGGLSAAPARAVSAVKNINLPEMPRNINIGDLNIKVPNLSQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7M6</accession>
    <name>A0A4W2C7M6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuronal pentraxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NPTX2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383200.1">
        <property type="nucleotide sequence ID" value="XM_027527399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7M6"/>
    <dbReference type="SMR" id="A0A4W2C7M6"/>
    <dbReference type="STRING" id="30522.A0A4W2C7M6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043808.1">
        <property type="protein sequence ID" value="ENSBIXP00000008828.1"/>
        <property type="gene ID" value="ENSBIXG00000014578.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019236.1">
        <property type="protein sequence ID" value="ENSBIXP00005010703.1"/>
        <property type="gene ID" value="ENSBIXG00005015270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883562"/>
    <dbReference type="CTD" id="4885"/>
    <dbReference type="GeneTree" id="ENSGT01060000248591"/>
    <dbReference type="OMA" id="AWKNEVG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008306">
        <property type="term" value="P:associative learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF1">
        <property type="entry name" value="NEURONAL PENTRAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610400" evidence="9">
        <location>
            <begin position="18"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="10">
        <location>
            <begin position="223"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="124"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="431" mass="47153" checksum="9F1D2A6DB6974BC3" modified="2019-09-18" version="1">MLALLAAGVVLAVAAGAQDGPAPSNRFVCTALPPEAARAGCPLPAMPMQGGALSPEEELRAAVLQLRETVVQQKETLGSQREAIRELTAKLARCEGLAGGKAPGRAASGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAVLPGDFREVLQRRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESALNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVLILGQEQDTVGGRFDATQAFVGELSQFNIWDRVLRAQEIINIANCSTNMPGNIIPWVDNNVDVFGGASKWPVETCEERLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8U2</accession>
    <name>A0A4W2C8U2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transmembrane protein 93</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EMC6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375371.1">
        <property type="nucleotide sequence ID" value="XM_027519570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C8U2"/>
    <dbReference type="STRING" id="30522.A0A4W2C8U2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017412.1">
        <property type="protein sequence ID" value="ENSBIXP00000009274.1"/>
        <property type="gene ID" value="ENSBIXG00000014988.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005409.1">
        <property type="protein sequence ID" value="ENSBIXP00005005922.1"/>
        <property type="gene ID" value="ENSBIXG00005011492.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878460"/>
    <dbReference type="CTD" id="83460"/>
    <dbReference type="GeneTree" id="ENSGT00390000003917"/>
    <dbReference type="OMA" id="MKANFEW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903349">
        <property type="term" value="C:omegasome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045050">
        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008504">
        <property type="entry name" value="Emc6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="110" mass="12031" checksum="5120A1EFA1B32D48" modified="2019-09-18" version="1">MAAVVAKREGPPFISEAAVRGNAAVLDYCRTSVSALSGATAGILGLTGLYGFIFYLLASILLSLLLILKAGRRWNKYFKSRRPLFTGGLIGGLFTYVLFWTFLYGMVHVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9K6</accession>
    <name>A0A4W2C9K6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Casein kinase II subunit alpha</fullName>
            <shortName evidence="13">CK II alpha</shortName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CSNK2A2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027372224.1">
        <property type="nucleotide sequence ID" value="XM_027516423.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027372225.1">
        <property type="nucleotide sequence ID" value="XM_027516424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C9K6"/>
    <dbReference type="SMR" id="A0A4W2C9K6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017903.1">
        <property type="protein sequence ID" value="ENSBIXP00000009564.1"/>
        <property type="gene ID" value="ENSBIXG00000015224.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025122.1">
        <property type="protein sequence ID" value="ENSBIXP00005014656.1"/>
        <property type="gene ID" value="ENSBIXG00005007659.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876791"/>
    <dbReference type="CTD" id="1459"/>
    <dbReference type="GeneTree" id="ENSGT00390000004215"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905818">
        <property type="term" value="P:regulation of chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF34">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="40"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="350" mass="41171" checksum="C5FA214716277F5B" modified="2019-09-18" version="1">MPGPAAGSRARVYAEVNSLRSREYWDYEAHVPSWGNQDDYQLVRKLGRGKYSEVFEAINITNNERVVVKILKPVKKKKIKREVKILENLRGGTNIIKLIDTVKDPVSKTPALVFEYINNTDFKQLYQILTDFDIRFYMYELLKALDYCHSKGIMHRDVKPHNVMIDHQQKKLRLIDWGLAEFYHPAQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGQDNYDQLVRIAKVLGTDELYGYLKKYHIDLDPHFNDILGQHSRKRWENFIHSENRHLVSPEALDLLDKLLRYDHQQRLTAKEAMEHPYFYPVVKEQSQPCADNAVLSSGLTAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAP8</accession>
    <name>A0A4W2CAP8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exocyst complex component 3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EXOC3L1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">As part of the exocyst, may play a role in regulated exocytosis of insulin granules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with EXOC2, EXOC4 and EXOC5; may be part of the exocyst.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAP8"/>
    <dbReference type="SMR" id="A0A4W2CAP8"/>
    <dbReference type="STRING" id="30522.A0A4W2CAP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044378.1">
        <property type="protein sequence ID" value="ENSBIXP00000009913.1"/>
        <property type="gene ID" value="ENSBIXG00000015533.1"/>
    </dbReference>
    <dbReference type="OMA" id="REQEPNT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000001">
        <property type="entry name" value="Exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000009">
        <property type="entry name" value="Exocyst complex component 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="739" mass="81325" checksum="D1A01DB85EAABFFD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAR5</accession>
    <name>A0A4W2CAR5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAR5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018480.1">
        <property type="protein sequence ID" value="ENSBIXP00000009933.1"/>
        <property type="gene ID" value="ENSBIXG00000002266.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="52"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="344" mass="39592" checksum="3350FA4F893804DE" modified="2019-09-18" version="1">MPQLKIPHAKAKTQHSQKKEKNKQKKKYIYIVKLFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTNALISHASKVMLKILQVRLQQYVNRELPDVQAGFRKGRGTRDQIANICWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNRYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNTNNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGSITSWEIDGETVETVSEFILGGSKITADGDCSHEIKRLTPWKESYDQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEQ5</accession>
    <name>A0A4W2CEQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Corticotropin-releasing factor receptor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CRHR1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027374073.1">
        <property type="nucleotide sequence ID" value="XM_027518272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044486.1">
        <property type="protein sequence ID" value="ENSBIXP00000010144.1"/>
        <property type="gene ID" value="ENSBIXG00000015703.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877781"/>
    <dbReference type="CTD" id="1394"/>
    <dbReference type="OMA" id="PQCNALV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015056">
        <property type="term" value="F:corticotrophin-releasing factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051424">
        <property type="term" value="F:corticotropin-releasing hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043404">
        <property type="term" value="F:corticotropin-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15445">
        <property type="entry name" value="7tmB1_CRF-R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000021">
        <property type="entry name" value="Corticotropin releasing factor receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000007">
        <property type="entry name" value="Corticotropin-releasing factor receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003052">
        <property type="entry name" value="GPCR_2_CRF1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003051">
        <property type="entry name" value="GPCR_2_CRF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF2">
        <property type="entry name" value="CORTICOTROPIN-RELEASING FACTOR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01279">
        <property type="entry name" value="CRFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01280">
        <property type="entry name" value="CRFRECEPTOR1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021346404" description="Corticotropin-releasing factor receptor 1" evidence="16">
        <location>
            <begin position="24"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="17">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="18">
        <location>
            <begin position="119"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="415" mass="47673" checksum="F6EC96866BBE2FEA" modified="2019-09-18" version="1">MGRCPQLRLVKALLLLGLNSISASLQDQHCESLSVASNVSGLQCNASVDLIGTCWPQSPAGQLVVRPCPAYFYGVRYNTTNNGYRECLANGTWAARVNYSECQEILSEEKKSKVHYHIAVIINYLGHCISLAALLVAFVLFLRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGWCRLVTAAYNYFHVTNFFWMFGEGCYLHTAIVLTYSTDRLRKWMFICIGWGVPFPIIVAWAIGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVLLINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFLESFQGFFVSVFYCFLNSEVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI04</accession>
    <name>A0A4W2CI04_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CI04"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045179.1">
        <property type="protein sequence ID" value="ENSBIXP00000011540.1"/>
        <property type="gene ID" value="ENSBIXG00000016772.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="70"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="683" mass="80154" checksum="E490273357ACD7EF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI07</accession>
    <name>A0A4W2CI07_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CI07"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004746.1">
        <property type="protein sequence ID" value="ENSBIXP00000005940.1"/>
        <property type="gene ID" value="ENSBIXG00000012081.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="511" mass="58896" checksum="2F861C028E1D9839" modified="2019-09-18" version="1">MGKTRDFFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITHREPDILECEVKWALESITTNKASGGDGIPVEPFQILKDDAVKVLHSICQQIWKTQQWPQDWKKSVFIPVPKKGNAKECSNYRTIALISHTSKVMLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANICWIMEKAREFQKNIYFCFIDYAKAFDCVHHNKLCKILQEMGIPNHLFCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVRVESEKVGLKLNIQKTIMASGPTTSWEIDGETVETVSDFLLLGSKITADGDCSHEIKRPLLLGRKVMTKLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGTLCEELTHWKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIP7</accession>
    <name>A0A4W2CIP7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interferon-induced very large GTPase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIP7"/>
    <dbReference type="STRING" id="30522.A0A4W2CIP7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023448.1">
        <property type="protein sequence ID" value="ENSBIXP00000012978.1"/>
        <property type="gene ID" value="ENSBIXG00000017982.1"/>
    </dbReference>
    <dbReference type="OMA" id="GPWIPYK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796:SF6">
        <property type="entry name" value="INTERFERON-INDUCED VERY LARGE GTPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796">
        <property type="entry name" value="URG4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="41319" checksum="972A751CEBDCB10C" modified="2019-09-18" version="1">MSFKISCQGATSIKTFVDFLWKKLTPAVSSTIRKNTALKIAGDIRTTCRAFNENRANLEKHILISLAEEENFDNYWQYLHNPKSYFKNYIENHVKRYFSDTGSEKIKTFLQMSLDDIKNAILSAMHASTAIAKDKSSTVSGWLDLFCDHLGSTLIFPRKDLISIEHQEIKDIEFIKEAMSKALEPEMKKVEQNCLSRLVEEMIPEIQKMLSEHLCGCWKQCPFCKAICTNTIPTHEGDHSAPFHRPKALNGWGWYQTDYFTINYCTSSVASDRFFILNDDRRIPYKTYRQAGGEYATWSITPDTSMQPYWKWFVCHFRSKLEEKYHRKFINTGEIPDAWKKITKQEVLDDLKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK26</accession>
    <name>A0A4W2CK26_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904916</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CK26"/>
    <dbReference type="STRING" id="30522.A0A4W2CK26"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022118.1">
        <property type="protein sequence ID" value="ENSBIXP00000012290.1"/>
        <property type="gene ID" value="ENSBIXG00000017370.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033765.1">
        <property type="protein sequence ID" value="ENSBIXP00005040744.1"/>
        <property type="gene ID" value="ENSBIXG00005006002.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286967"/>
    <dbReference type="OMA" id="KYCNKSQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF168">
        <property type="entry name" value="OLFACTORY RECEPTOR 51A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="314" mass="35671" checksum="C21CBEFB7A41C91A" modified="2019-09-18" version="1">MSIINISHAEITTFILVGMPGLEYAHIWISIPICNMYLIALLGNCTILFIIKTEPSLHEPMYYFLSMLTLSDMGLSFSSLPTMLRIFLFKASEISANACFAQQFFIHGFTALESSVLLIMSFDRFLAIHNPLRYSSLLTTLRVAQIGIVCFFKSFLLVLPFPFTLRMLKYCEKRQLSHSYCLHQDVMKLACSDNQIDVIYGFFGALCLMVDFMLIAVSYILILKTVLGIASQKEQLKALNTCVSHICAVIIFYLPIINLAIVHRFVQHVSPLFNVLMANVLLLVPPLMNPIVYCVKTRQIRVKVVAKLCQKQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLN7</accession>
    <name>A0A4W2CLN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="15">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
            <location evidence="1 15">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLN7"/>
    <dbReference type="SMR" id="A0A4W2CLN7"/>
    <dbReference type="STRING" id="30522.A0A4W2CLN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023015.1">
        <property type="protein sequence ID" value="ENSBIXP00000012871.1"/>
        <property type="gene ID" value="ENSBIXG00000003059.1"/>
    </dbReference>
    <dbReference type="OMA" id="QNQVKKW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005677">
        <property type="term" value="C:chromatin silencing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046015">
        <property type="term" value="P:regulation of transcription by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000068">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 15" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="50"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="141"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="460" mass="51219" checksum="63F3D060547D0117" modified="2019-09-18" version="1">MFEEPEWAEEAPVVADLGSVALGLRPTAVSQIKGSNRRQLLATLRALEVASLPQQSPSLPGSDSEEEEEVVETKKKRPKKGLFAGASNEVEEKRKKKRHKRGPPSSDSKEEEVEREKCHKGALLGSDLAEEEKKKRKCQKQIPSNLAQPLDSVDQTGSKAWNSSATSDPTKPTCETPSSNPPHTLSRKQWRNRQKNKRRQKNKFRPSQPPEQAPAPAPASAEEAEVPSAPSPDNHGARAEALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQNQVKKWPLQPVDRIARDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESIDVAVFCLSLMGTNIRDFLEEANRVLKPGGLLKVAEVSSRFEDVRTFLGAVTKLGFKVISKDLTNSHFFLFDFEKTGPPRVGPTTQLSGLKLQPCLYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMV0</accession>
    <name>A0A4W2CMV0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZFP28 zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZFP28</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in transcriptional regulation. May have a role in embryonic development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMV0"/>
    <dbReference type="STRING" id="30522.A0A4W2CMV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023634.1">
        <property type="protein sequence ID" value="ENSBIXP00000013297.1"/>
        <property type="gene ID" value="ENSBIXG00000018252.1"/>
    </dbReference>
    <dbReference type="OMA" id="FNKSGRW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
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                <person name="Tearle R."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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            <topology evidence="2">Multi-pass membrane protein</topology>
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                <person name="Tearle R."/>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
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        <location>
            <begin position="91"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="517" mass="59759" checksum="C5ABD4BB272F7D14" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW81</accession>
    <name>A0A4W2CW81_BOBOX</name>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <subcellularLocation>
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        <text evidence="2">Belongs to the DAP10 family.</text>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR21409:SF1">
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    </dbReference>
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            <end position="93"/>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003955.1"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="93" mass="9624" checksum="65D60DBB6DD0C76E" modified="2019-09-18" version="1">MVPPGNILFLLLLPVATAQMTPGEKSTPTPLHGITSGSCSGCGPLSLPLLAGLVAADAVVSLLIVVVVFVCARLRSRPTQEDDKIYINMPGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXG9</accession>
    <name>A0A4W2CXG9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prostaglandin G/H synthase 1</fullName>
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        <alternativeName>
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        </alternativeName>
        <alternativeName>
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        </alternativeName>
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            <fullName evidence="31">Prostaglandin-endoperoxide synthase 1</fullName>
        </alternativeName>
    </protein>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="43 44" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="43" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="43" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="1">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = prostaglandin G2</text>
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            <dbReference type="ChEBI" id="CHEBI:15379"/>
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        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:42597"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:23728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
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            <dbReference type="ChEBI" id="CHEBI:17499"/>
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            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="1.14.99.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:23729"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>(9Z,12Z)-octadecadienoate + AH2 + O2 = (13R)-hydroxy-(9Z,11E)-octadecadienoate + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:75455"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
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            <dbReference type="ChEBI" id="CHEBI:15379"/>
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            <dbReference type="ChEBI" id="CHEBI:136655"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:75456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(9Z,12Z)-octadecadienoate + AH2 + O2 = (13S)-hydroxy-(9Z,11E)-octadecadienoate + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:75451"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
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            <dbReference type="ChEBI" id="CHEBI:90850"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:75452"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(9Z,12Z)-octadecadienoate + AH2 + O2 = (9R)-hydroxy-(10E,12Z)-octadecadienoate + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:75447"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
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            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:77895"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:75448"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(9Z,12Z)-octadecadienoate + AH2 + O2 = (9S)-hydroxy-(10E,12Z)-octadecadienoate + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:75459"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:77852"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:75460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>prostaglandin G2 + AH2 = prostaglandin H2 + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:42600"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:82629"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:42601"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; prostaglandin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the prostaglandin G/H synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="40">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXG9"/>
    <dbReference type="STRING" id="30522.A0A4W2CXG9"/>
    <dbReference type="GlyCosmos" id="A0A4W2CXG9">
        <property type="glycosylation" value="3 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000028616.1">
        <property type="protein sequence ID" value="ENSBIXP00000016610.1"/>
        <property type="gene ID" value="ENSBIXG00000020753.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00662"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004666">
        <property type="term" value="F:prostaglandin-endoperoxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019371">
        <property type="term" value="P:cyclooxygenase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09816">
        <property type="entry name" value="prostaglandin_endoperoxide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000002">
        <property type="entry name" value="Prostaglandin G/H synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000235">
        <property type="entry name" value="Prostaglandin G/H synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050783">
        <property type="entry name" value="Oxylipin_biosynth_metab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903">
        <property type="entry name" value="PROSTAGLANDIN G/H SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903:SF6">
        <property type="entry name" value="PROSTAGLANDIN G_H SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="26" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 40" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="27" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="28" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 39" id="KW-0349">Heme</keyword>
    <keyword evidence="23 39" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="24" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="25" id="KW-0472">Membrane</keyword>
    <keyword evidence="16 39" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0492">Microsome</keyword>
    <keyword evidence="22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="10" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="44" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="42">
        <location>
            <begin position="67"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="41">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="41">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="38">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" description="For cyclooxygenase activity" evidence="38">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="39">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00020404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00031217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00031794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00033143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00035976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00036313"/>
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    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00036358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00036409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00047673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00048310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="43">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="635" mass="72415" checksum="3704CE5A3A57DE15" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZU4</accession>
    <name>A0A4W2CZU4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 38 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SLC38A1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027398452.1">
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        <property type="gene ID" value="ENSBIXG00000021441.1"/>
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        <property type="gene ID" value="ENSBIXG00005006278.1"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:basolateral plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0015186">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009449">
        <property type="term" value="P:gamma-aminobutyric acid biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032228">
        <property type="term" value="P:regulation of synaptic transmission, GABAergic"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="AA_transpt_TM"/>
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        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF184">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID SYMPORTER 1"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
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            <end position="125"/>
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        <location>
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        <location>
            <begin position="452"/>
            <end position="474"/>
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        <location>
            <begin position="70"/>
            <end position="470"/>
        </location>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="486" mass="54029" checksum="FC1F97E1D347E566" modified="2019-09-18" version="1">MMHFKSGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSKFISDRESRRSLTNSHLEKKKCDDYIPGTTSLGMSVFNLSNAIMGSGILGLAFALANTGILLFLVLLTSVTLLSIYSINLLLICSKETGCMVYEKLGEQVFGTTGKLVIFGATSLQNTGAMLSYLFIVKNELPSAIKFLMGKEEEFSAWYVDGRLLVVVVTFGIILPLCLLKNLGYLGYTSGFSLSCMVFFLIVVIYKKFKITCSVPELNSTIPNSTNPDMCTPKYVTFNSKTVYALPTIAFAFVCHPSVLPIYSELKDRSQKKMQMVSNISFFAMFVMYFLTAIFGYLTFYESVQSDLLHKYQSKNDILILTVRLAVIMAVILTVPVLFFTVRSSLFELAKKTKFNLCRHVLVTILLLVIINLLVIFIPSMKDIFGVVGVTSANMLIFILPSSLYLKITSQDGDKGTQQIWAALFLGLGVLFSLVSIPLVIYDWAYSSSGDEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D488</accession>
    <name>A0A4W2D488_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alpha-mannosidase</fullName>
            <ecNumber evidence="17">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">MAN2A1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 H2O = 2 alpha-D-mannopyranose + an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:56052"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28729"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="3.2.1.114"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D488"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032417.1">
        <property type="protein sequence ID" value="ENSBIXP00000019015.1"/>
        <property type="gene ID" value="ENSBIXG00000022736.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTDILCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004572">
        <property type="term" value="F:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048534">
        <property type="entry name" value="Man2a1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF69">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21260">
        <property type="entry name" value="Laman-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="19">
        <location>
            <begin position="503"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1145" mass="130869" checksum="749BC80A7911D412" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4Z2</accession>
    <name>A0A4W2D4Z2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4Z2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035360.1">
        <property type="protein sequence ID" value="ENSBIXP00000020934.1"/>
        <property type="gene ID" value="ENSBIXG00000003896.1"/>
    </dbReference>
    <dbReference type="OMA" id="NNFRYAA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="225"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="616" mass="70857" checksum="B0F2104CED13656B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCN0</accession>
    <name>A0A4W2DCN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC113890548</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCN0"/>
    <dbReference type="STRING" id="30522.A0A4W2DCN0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036854.1">
        <property type="protein sequence ID" value="ENSBIXP00000021948.1"/>
        <property type="gene ID" value="ENSBIXG00000024934.1"/>
    </dbReference>
    <dbReference type="OMA" id="PVTMCPG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15419">
        <property type="entry name" value="7tmA_OR9K2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="46"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="316" mass="34765" checksum="AA982A6E83495ED2" modified="2019-09-18" version="1">MSPHRNGNLSMMPLQEFVLDGFEGGLQTQALLFALFLALYVVAILGNLTMIMVITLDARLHSPMYFFLKNLSLVDLCYSSVIAPKALANFLSSSKVITFEGCATQFFFFSLLVTTEGFLLAVMAYDRFMAICSPLSYPISMCPSVCARLMLGCYCGGCLNSILQASFTFTLPFCSSNHIDHFFCDVPPLLKLACADTTINELVMFGICGLIIMGTTLVVLTSYGYVTVTILRMCSGGGRHKLFSTCGSHMTAVSLFYGTVFVMYAQPGAVESMEQGKVFSVFYTLVIPMLNPLIYSLRNKDVKDALWRLGQKHTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEK6</accession>
    <name>A0A4W2DEK6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="33">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NOTCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2DEK6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040830.1">
        <property type="protein sequence ID" value="ENSBIXP00000024599.1"/>
        <property type="gene ID" value="ENSBIXG00000026844.1"/>
    </dbReference>
    <dbReference type="OMA" id="QNINDCD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001709">
        <property type="term" value="P:cell fate determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903706">
        <property type="term" value="P:regulation of hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="CDD" id="cd21704">
        <property type="entry name" value="JMTM_Notch3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000172">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000151">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000005">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000080">
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <authorList>
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        <property type="entry name" value="Collagen"/>
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    </dbReference>
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        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
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        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="295"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="111"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="390" mass="40086" checksum="001621F6A303C169" modified="2019-09-18" version="1">MENEEILKEKELLASTEDGTALDQTMLSRMETFEVNDPKPKRRNGMNCIMVLVILYLVLLTAGAGLLVIQVLNLQERLRVLEMPYNNETQAQLTQVVTKQETLQQRMDNFIQIPGPPGPRGPPGIKGEAGRKGDMGMKGDMGPPGARGDKGDSGKPGFPGVAGIPGIKGDQGPPGMKGLPGFPGAVGSPGAKGDTGSIGPTGPIGPPGLPGTPGVEGVKGSKGDTGLQGQKGTKGESGFPGLSGVKGEKGSPGLGGLKGAPGPTGQKGEPGSRGATGPQGAKGEKGQKGDSSLSVRIIGSRNRGRAEVFYNGAWGTICDDSWDGSDATVFCRMLGYSSGSPVYNVGAGSGNIWLDDVACSGSEWTLWDCNKSSWGSHNCNHNEDAGVNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMQ5</accession>
    <name>A0A4W2DMQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Interleukin-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eosinophil differentiation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">IL5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer; disulfide-linked. Interacts with IL5RA. Interacts with CSF2RB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the IL-5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027404651.1">
        <property type="nucleotide sequence ID" value="XM_027548850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMQ5"/>
    <dbReference type="SMR" id="A0A4W2DMQ5"/>
    <dbReference type="STRING" id="30522.A0A4W2DMQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049280.1">
        <property type="protein sequence ID" value="ENSBIXP00000020176.1"/>
        <property type="gene ID" value="ENSBIXG00000023531.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023873.1">
        <property type="protein sequence ID" value="ENSBIXP00005036371.1"/>
        <property type="gene ID" value="ENSBIXG00005017893.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896628"/>
    <dbReference type="CTD" id="3567"/>
    <dbReference type="GeneTree" id="ENSGT00390000016991"/>
    <dbReference type="OMA" id="VPTHKNH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005137">
        <property type="term" value="F:interleukin-5 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030222">
        <property type="term" value="P:eosinophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038043">
        <property type="term" value="P:interleukin-5-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002639">
        <property type="term" value="P:positive regulation of immunoglobulin production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071803">
        <property type="term" value="P:positive regulation of podosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000186">
        <property type="entry name" value="IL-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48491">
        <property type="entry name" value="INTERLEUKIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48491:SF1">
        <property type="entry name" value="INTERLEUKIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02025">
        <property type="entry name" value="IL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00432">
        <property type="entry name" value="INTERLEUKIN5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516453" description="Interleukin-5" evidence="12">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="134" mass="15229" checksum="0B3A2328EE7431F4" modified="2019-09-18" version="1">MRMHLHLTLVALGAAYVCANAVESTMNRLVAETLTLLSSHRTLLIGDGNLMIPTPQHTNHQLCIEEVFQGIDTLKNQTAQGDAVKKIFQNLSLIKEYIDLQKRKCGGERWRVKQFLDYLQVFLGVINTEWTMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNE6</accession>
    <name>A0A4W2DNE6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4637 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNE6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012154.1">
        <property type="protein sequence ID" value="ENSBIXP00000027831.1"/>
        <property type="gene ID" value="ENSBIXG00000008803.1"/>
    </dbReference>
    <dbReference type="OMA" id="HCVLEHP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029174">
        <property type="entry name" value="DUF4637"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37878:SF1">
        <property type="entry name" value="DUF4637 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37878">
        <property type="entry name" value="HYPOTHETICAL PROTEIN LOC689039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15470">
        <property type="entry name" value="DUF4637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4637" evidence="2">
        <location>
            <begin position="122"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="177" mass="19431" checksum="259A92A0182170C3" modified="2019-09-18" version="1">MDKHGAKAPLWKKELEEPRAREAEAEAEAAEEESESESESDEERPPEESAAEGEGPAEAEGGEGRERGSVSYYPLRQESSTQQVALLRRAESGFWGWFSPLALLGGLAAPADRKRSPPEEPCVLETRRRRPRGGGCARCEILFCKKCRNLHSSPSYVAHCVLEHPDLGKTRPAHGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNU3</accession>
    <name>A0A4W2DNU3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="20">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ATP2B1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts. Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells. May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Presynaptic cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNU3"/>
    <dbReference type="SMR" id="A0A4W2DNU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034778.1">
        <property type="protein sequence ID" value="ENSBIXP00000020603.1"/>
        <property type="gene ID" value="ENSBIXG00000023899.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000001">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000002">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000001">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000002">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000007">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022141">
        <property type="entry name" value="ATP_Ca_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF245">
        <property type="entry name" value="PLASMA MEMBRANE CALCIUM-TRANSPORTING ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12424">
        <property type="entry name" value="ATP_Ca_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 20" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="932"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="969"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1007"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1040"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="297"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1160"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1200"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1220" mass="134785" checksum="B595540F496F45D5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP64</accession>
    <name>A0A4W2DP64_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OCEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DP64"/>
    <dbReference type="SMR" id="A0A4W2DP64"/>
    <dbReference type="STRING" id="30522.A0A4W2DP64"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052579.1">
        <property type="protein sequence ID" value="ENSBIXP00000025931.1"/>
        <property type="gene ID" value="ENSBIXG00000028033.1"/>
    </dbReference>
    <dbReference type="OMA" id="LENQARC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF15">
        <property type="entry name" value="OCCLUDIN_ELL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OCEL" evidence="3">
        <location>
            <begin position="20"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="137" mass="15723" checksum="4ECB63BF7BB93AF3" modified="2019-09-18" version="1">AAPDLGRGVAPSGHRNLSCKDYELKYPPVSTAKDRSRYAAVFQDQYPEFLELQQEVGSAQAKLQQLEALLNSLPRPRSQKEAHVAARVWREFEKKQMDPSFLDKQARCHYLKGKLRHLKTQIQKFDDQGESEGSVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DPK4</accession>
    <name>A0A4W2DPK4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YPEL2</name>
        <name evidence="4" type="synonym">YPEL</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375309.1">
        <property type="nucleotide sequence ID" value="XM_027519508.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027375311.1">
        <property type="nucleotide sequence ID" value="XM_027519510.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027375312.1">
        <property type="nucleotide sequence ID" value="XM_027519511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DPK4"/>
    <dbReference type="SMR" id="A0A4W2DPK4"/>
    <dbReference type="STRING" id="30522.A0A4W2DPK4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010886.1">
        <property type="protein sequence ID" value="ENSBIXP00000028252.1"/>
        <property type="gene ID" value="ENSBIXG00000009372.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036704.1">
        <property type="protein sequence ID" value="ENSBIXP00005022380.1"/>
        <property type="gene ID" value="ENSBIXG00005025296.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878436"/>
    <dbReference type="CTD" id="388403"/>
    <dbReference type="GeneTree" id="ENSGT00940000159010"/>
    <dbReference type="OMA" id="SSRIYGC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="19"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="119" mass="13577" checksum="5904018C67E1A2D2" modified="2019-09-18" version="1">MVKMTRSKTFQAYLPSCHRTYSCIHCRAHLANHDELISKSFQGSQGRAYLFNSVVNVGCGPAEERVLLTGLHAVADIYCENCKTTLGWKYEHAFESSQKYKEGKYIIELAHMIKDNGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQJ3</accession>
    <name>A0A4W2DQJ3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">METTL16</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with MEPCE. Interacts with LARP7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the methyltransferase superfamily. METTL16/RlmF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027375606.1">
        <property type="nucleotide sequence ID" value="XM_027519805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQJ3"/>
    <dbReference type="SMR" id="A0A4W2DQJ3"/>
    <dbReference type="STRING" id="30522.A0A4W2DQJ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000349.1">
        <property type="protein sequence ID" value="ENSBIXP00000026746.1"/>
        <property type="gene ID" value="ENSBIXG00000029477.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003068.1">
        <property type="protein sequence ID" value="ENSBIXP00005007101.1"/>
        <property type="gene ID" value="ENSBIXG00005012438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878575"/>
    <dbReference type="CTD" id="79066"/>
    <dbReference type="GeneTree" id="ENSGT00390000016694"/>
    <dbReference type="OMA" id="TEFCQGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120048">
        <property type="term" value="F:U6 snRNA (adenine-(43)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030629">
        <property type="term" value="F:U6 snRNA 3'-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905869">
        <property type="term" value="P:negative regulation of 3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120049">
        <property type="term" value="P:snRNA (adenine-N6)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000062">
        <property type="entry name" value="U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017182">
        <property type="entry name" value="METTL16/PsiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037350">
        <property type="entry name" value="Mtase_ZK1128_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="403"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037350-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="561" mass="63184" checksum="67054A9AB0437C6B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRH7</accession>
    <name>A0A4W2DRH7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Ras-related protein Rab-34</fullName>
            <ecNumber evidence="7">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="27">Interacts with RILP. The GTP-bound form interacts with REP15.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Phagosome membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRH7"/>
    <dbReference type="STRING" id="30522.A0A4W2DRH7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014506.1">
        <property type="protein sequence ID" value="ENSBIXP00000027101.1"/>
        <property type="gene ID" value="ENSBIXG00000028796.1"/>
    </dbReference>
    <dbReference type="OMA" id="SCANEFE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04108">
        <property type="entry name" value="Rab36_Rab34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000748">
        <property type="entry name" value="ras-related protein Rab-34 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="22" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-0636">Prenylation</keyword>
    <keyword evidence="16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00062850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00067829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="360" mass="39972" checksum="E97A83D1DA171B3C" modified="2019-09-18" version="1">MNILAPVRRDRVLTKLPQVGARATSGFGRRWGWAGRRRHRTGLGPNPAALLGQLLLGGPFPTPTLPPRPGLLGGGLRARKWVSSILFHSTQCLRKEAALHVHKDFQPRVTCACQEHRTGTVGFKISKVIVVGDLSVGKTCLINRFCKDTFDKNYKATIGVDFEMERFEVLGVPFSLQLWDTAGQERFKCIASTYYRGAQAIIIVFNLNDVASLEHTKQWLADALKENDPSSVLLFLVGSKKDLSVSVPVVGEDFTSRQGKPHAQHLTLTFLQTPAQYILMEKDALKVAQEMKAEYWAVSSLTGENVQEFFFRVAALTFEVNVLAELEKSGSRRIGDVVRINSDDSNLYLTASKKKPTCCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTT6</accession>
    <name>A0A4W2DTT6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDC42 small effector protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with CDC42 (in GTP-bound form). Interacts weakly with RAC1 and not at all with RHOA.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTT6"/>
    <dbReference type="STRING" id="30522.A0A4W2DTT6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050484.1">
        <property type="protein sequence ID" value="ENSBIXP00000022460.1"/>
        <property type="gene ID" value="ENSBIXG00000005165.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015182.1">
        <property type="protein sequence ID" value="ENSBIXP00005000951.1"/>
        <property type="gene ID" value="ENSBIXG00005029678.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000188436"/>
    <dbReference type="OMA" id="CMVEKPE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039056">
        <property type="entry name" value="SPEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502:SF3">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="6">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="80" mass="8859" checksum="E2B78B07ADB11EFC" modified="2019-09-18" version="1">ASEECEGIWDKPGCCMVEKPELNKRSRIDWAVVGEPMNFVHLIHIGPGDMRAGDGLATTGASQGQLRPRELKPWTLSDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DU58</accession>
    <name>A0A4W2DU58_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proline rich transmembrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PRRT1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DU58"/>
    <dbReference type="STRING" id="30522.A0A4W2DU58"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004963.1">
        <property type="protein sequence ID" value="ENSBIXP00000030135.1"/>
        <property type="gene ID" value="ENSBIXG00000011971.1"/>
    </dbReference>
    <dbReference type="OMA" id="PYNNPLQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051423">
        <property type="entry name" value="CD225/Dispanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948">
        <property type="entry name" value="NG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948:SF21">
        <property type="entry name" value="PROLINE-RICH TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="305" mass="31362" checksum="2D23B4320B62F36C" modified="2019-09-18" version="1">MSSEKSGLPDSVPHTSPPPYNAPQPPAEPPAPPPQTAPSSHHHHHHHYHQSGTATLPRLGAGGLASGAAAQRGPSSSATLPRPPHHAPPGPAAGAPPPGCATLPRMPPDPYLQETRFEGPLPPPPPAAAAPPPPATSHTAQAPGFVVPTHTGAVGTLPLGGYVAPGYPLQLQPCTAYVPVYPVGTPYAGATPGGTGVTSTLSPPPQGPGLALLEPRRPPHDYMPIAVLTTICCFWPTGIIAIFKAVQVRTALARGDMVSAEIASREARNFSFISLAVGIAAMVLCTILTVVIIIAAQHHENYWDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYZ1</accession>
    <name>A0A4W2DYZ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Mitochondrial basic amino acids transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Carnitine/acylcarnitine translocase-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Mitochondrial carnitine/acylcarnitine carrier protein CACL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Mitochondrial ornithine transporter 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 25 member 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">SLC25A29</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-histidine(out) = L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:72807"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-homoarginine(in) + L-arginine(out) = L-homoarginine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:72799"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:143006"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysine(out) + L-arginine(in) = L-lysine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:70827"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-ornithine(in) + L-arginine(out) = L-ornithine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:34991"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(omega)-methyl-L-arginine(in) + L-arginine(out) = N(omega)-methyl-L-arginine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:72803"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 26">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376595.1">
        <property type="nucleotide sequence ID" value="XM_027520794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYZ1"/>
    <dbReference type="SMR" id="A0A4W2DYZ1"/>
    <dbReference type="STRING" id="30522.A0A4W2DYZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043508.1">
        <property type="protein sequence ID" value="ENSBIXP00000031694.1"/>
        <property type="gene ID" value="ENSBIXG00000014084.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021030.1">
        <property type="protein sequence ID" value="ENSBIXP00005011909.1"/>
        <property type="gene ID" value="ENSBIXG00005016264.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879426"/>
    <dbReference type="CTD" id="123096"/>
    <dbReference type="GeneTree" id="ENSGT00940000157766"/>
    <dbReference type="OMA" id="VYRESGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005289">
        <property type="term" value="F:high-affinity L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005292">
        <property type="term" value="F:high-affinity lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089709">
        <property type="term" value="P:L-histidine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000037">
        <property type="entry name" value="Solute carrier family 25 member 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF61">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="10 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 25 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 26" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="90"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="190"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00079387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00080567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="298" mass="31866" checksum="CA830A3343DC23F5" modified="2019-09-18" version="1">MALDFLAGCAGGVAGVLVGHPFDTVKVRLQVQSVEKPQYRGTLHCFQAIIKQESVLGLYRGLGSPLLGLTFINALVFGVQGNTLRALGRDSPLNQFLAGAAAGAIQCVICCPMELAKTRLQLQEAGPARTYRGPLDCLAQIYRQEGLRGVNRGMVSTLLRETPSFGVYFLTYDVLTRALGCEPGDRLLVPKLLLAGGTSGIASWLSTYPVDVVKSRLQADGLQGAPRYRGIVDCVQQSYREEGWRVFTRGLASTLLRAFPVNAATFATVTVVLSYARGEEARLEGDAGSAALAQPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZF1</accession>
    <name>A0A4W2DZF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041020.1">
        <property type="protein sequence ID" value="ENSBIXP00000024714.1"/>
        <property type="gene ID" value="ENSBIXG00000026841.1"/>
    </dbReference>
    <dbReference type="OMA" id="DECQAGI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="42802" checksum="E7B40D1F2F1A4EBF" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKVFDCVDHNKLWKILQEMGIPDHLICFLRNLYAGQEATVRTGHGTTDWFQIGKGVHQGCTLSPCLFNLYAEYIMRNAGLEEAQAGIKFAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKTMASGPITSWEIDGETVETVSDFSFLGSKITADGDCSHEIKRCFLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWIVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEVSPGISLEGMMLKLKLQYFGHLMRRVDSWEKTLMLGGIGGRRRRGRQRMRWLAGITDSMDMSLSELQELVMDREAWRAGIHGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0E1</accession>
    <name>A0A4W2E0E1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Lysine-specific demethylase 2B</fullName>
            <ecNumber evidence="5">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">F-box and leucine-rich repeat protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="34">F-box protein FBL10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="35">F-box/LRR-repeat protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">JmjC domain-containing histone demethylation protein 1B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">[Histone-H3]-lysine-36 demethylase 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="41" type="primary">KDM2B</name>
    </gene>
    <organism evidence="41 42">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="41 42" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="41" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the JHDM1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="5"/>
    <dbReference type="SMR" id="A0A4W2E0E1"/>
    <dbReference type="STRING" id="30522.A0A4W2E0E1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017796.1">
        <property type="protein sequence ID" value="ENSBIXP00000032577.1"/>
        <property type="gene ID" value="ENSBIXG00000007381.1"/>
    </dbReference>
    <dbReference type="OMA" id="HTHLTHY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21785">
        <property type="entry name" value="CTD_KDM2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22180">
        <property type="entry name" value="F-box_FBXL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15644">
        <property type="entry name" value="PHD_KDM2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000083">
        <property type="entry name" value="Lysine (K)-specific demethylase 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1360:FF:000002">
        <property type="entry name" value="Lysine (K)-specific demethylase 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000005">
        <property type="entry name" value="lysine-specific demethylase 2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000020">
        <property type="entry name" value="lysine-specific demethylase 2B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000011">
        <property type="entry name" value="Lysine-specific demethylase 2B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041070">
        <property type="entry name" value="JHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17811">
        <property type="entry name" value="JHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16866">
        <property type="entry name" value="PHD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
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    <keyword evidence="24" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0433">Leucine-rich repeat</keyword>
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            <begin position="945"/>
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            <begin position="506"/>
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    <evidence type="ECO:0000256" key="16">
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    <evidence type="ECO:0000256" key="17">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="37">
        <source>
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        <source>
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        <source>
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    <sequence length="1424" mass="162064" checksum="8AFA4DD51833D56F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1Y4</accession>
    <name>A0A4W2E1Y4_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">Glutaryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="13">1.3.8.6</ecNumber>
        </recommendedName>
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        <name evidence="21" type="primary">GCDH</name>
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    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>glutaryl-CoA + oxidized [electron-transfer flavoprotein] + 2 H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13389"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid metabolism; lysine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid metabolism; tryptophan metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the acyl-CoA dehydrogenase family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000025621.1"/>
        <property type="gene ID" value="ENSBIXG00000027702.1"/>
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        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:mitochondrial matrix"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
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        <property type="entry name" value="GCD"/>
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        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
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    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
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    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052033">
        <property type="entry name" value="Glutaryl-CoA_DH_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807:SF1">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 17" id="KW-0274">FAD</keyword>
    <keyword evidence="5 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="20">
        <location>
            <begin position="61"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="19">
        <location>
            <begin position="176"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="18">
        <location>
            <begin position="288"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="438" mass="48472" checksum="C420C9901292CDEC" modified="2019-09-18" version="1">MALRGVYAQLLNRGPGLRVFRSWSSATAQTEKGEKTQSRSAKPSRPEFDWRDPLLLEEQLTADEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGMLGPTIQGYSCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMYPIYAYGSEEQKQKYLPRLAKGELLGCFGLTEPNHGSDPSGMETRARHNPSSRSYILSGSKTWITNSPVADLLIVWARCEDSCIRGFLLEKGMRGLSTPRIEGKFSLRASSTGMIIMDDVEVPEENVLPGVSGLAGPFGCLNNARYGITWGVLGAAEFCLHTARQYTLDRIQFGVPLAKNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHVMNLESVNTYEGTHDIHALILGRAITGIQAFVAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3P8</accession>
    <name>A0A4W2E3P8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 186</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM186 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3P8"/>
    <dbReference type="STRING" id="30522.A0A4W2E3P8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053939.1">
        <property type="protein sequence ID" value="ENSBIXP00000026269.1"/>
        <property type="gene ID" value="ENSBIXG00000030330.1"/>
    </dbReference>
    <dbReference type="OMA" id="MTIGDTG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026571">
        <property type="entry name" value="Tmem186"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="212" mass="23785" checksum="6CCD4F6951C1F12E" modified="2019-09-18" version="1">MAAVLRAVARSPGPAAWGRPLHRLCCCGGQDPRRWVGSGPPHSKEKPLGPETEKFQMVYRFDAIKAFGYLSRLKVAQTALTVAALPPGLYCYSQGLMPFSSLCLAGGVAGFALAMLCWMSHFFRRLVGILYVNEEGTVLRVAHLTFWGRRQDTYCPVADVIPMTESPDRPQELFMRIQQYSGKQTFYLTLRYGRVLDQERFTQVFGVLDALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6Y2</accession>
    <name>A0A4W2E6Y2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Retinol binding protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RBP5</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397009.1">
        <property type="nucleotide sequence ID" value="XM_027541208.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397010.1">
        <property type="nucleotide sequence ID" value="XM_027541209.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397011.1">
        <property type="nucleotide sequence ID" value="XM_027541210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6Y2"/>
    <dbReference type="SMR" id="A0A4W2E6Y2"/>
    <dbReference type="STRING" id="30522.A0A4W2E6Y2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016058.1">
        <property type="protein sequence ID" value="ENSBIXP00000031823.1"/>
        <property type="gene ID" value="ENSBIXG00000001677.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025215.1">
        <property type="protein sequence ID" value="ENSBIXP00005036956.1"/>
        <property type="gene ID" value="ENSBIXG00005003024.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892394"/>
    <dbReference type="CTD" id="83758"/>
    <dbReference type="GeneTree" id="ENSGT00940000162526"/>
    <dbReference type="OMA" id="NINMALR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19464">
        <property type="entry name" value="CRBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="5">
        <location>
            <begin position="7"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="135" mass="15962" checksum="562A10B255659D49" modified="2019-09-18" version="1">MPPNLTGYYRFVSQKNLEDYLQALNVNMALRKIALLLKPDKEIDQRGNHMTVKTLSTFRNYVLEFEVGVEFEEDLRTVDGRKCQTIVTWEEEQLVCVQKGEVPNRGWRLWLEEEMLYQEVTARDAVCQCVFRKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E700</accession>
    <name>A0A4W2E700_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial fission regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MTFR1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in mitochondrial aerobic respiration. May also regulate mitochondrial organization and fission.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Plays a role in mitochondrial aerobic respiration. Regulates mitochondrial organization and fission.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the MTFR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E700"/>
    <dbReference type="STRING" id="30522.A0A4W2E700"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022912.1">
        <property type="protein sequence ID" value="ENSBIXP00000034755.1"/>
        <property type="gene ID" value="ENSBIXG00000017837.1"/>
    </dbReference>
    <dbReference type="OMA" id="CPRIHFQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007972">
        <property type="entry name" value="Mtfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215:SF1">
        <property type="entry name" value="MITOCHONDRIAL FISSION REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215">
        <property type="entry name" value="PROTEIN OF UNKNOWN FUNCTION DUF729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05308">
        <property type="entry name" value="Mito_fiss_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="355" mass="38381" checksum="D3953F073A1F76F1" modified="2025-10-08" version="2">MVRLAGARGGDWVIVSNALGQPSFKPELPEQAPGCRAAEVLWSGKPYGSSRSIVRKIGTNLSLIQCPRVQFQLTSPTTEWSSSPSGEDAVVSFADVGWVAEEEGECSTRQRIEVRSRPTLQDDLPFFEKPHSRQPSLPTLSPEEPPLQTSTLANEEALQKICALESELAALRAQIAKIVTLQEQQNLSAGDLESTRPAAAAAPSVPPAPPLPPPPPPLPSPGLQQSTSAIDLIKERRRRNASAGKTPVKNSPKKTDLPNMLEILKDMNSVKLRSVKRSEQDTKPKPADAADPAALIAEALKKKFAYRYRSDSQGEAEKGIQKSESEATSERVLFGPHMLKSTGKMKALIENASTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E744</accession>
    <name>A0A4W2E744_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inactive serine protease 54</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Plasma kallikrein-like protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PRSS54</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E744"/>
    <dbReference type="STRING" id="30522.A0A4W2E744"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017921.1">
        <property type="protein sequence ID" value="ENSBIXP00000032613.1"/>
        <property type="gene ID" value="ENSBIXG00000015238.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025281.1">
        <property type="protein sequence ID" value="ENSBIXP00005014778.1"/>
        <property type="gene ID" value="ENSBIXG00005007663.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230389"/>
    <dbReference type="OMA" id="TDTAMQF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000125">
        <property type="entry name" value="inactive serine protease 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250">
        <property type="entry name" value="CHYMOTRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250:SF45">
        <property type="entry name" value="INACTIVE SERINE PROTEASE 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0721">Serine protease homolog</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="21"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="300"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022542"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="375" mass="42422" checksum="C2C633790CA6044C" modified="2019-09-18" version="1">MPSSIELGIISCGIQKSNIAVTSEEGLVEEQEFPWVVSLQDSQYTHLTFGSILSEFWILSIASTFQNRPDAVAIIGIAKMDAKLIAHEEYPINTIIIHEDFDNKTMRNNIALLKTDTAMQFNSLVRPICFLGRKLNRPPVLMNCWVAGWNPTSATGNHMTMSILRKISVKDIDSCPLNIEQKTGCGNHLEMETNAVCLGDPGNPMMCQLKEMSLWVLRGILSQGGEKCPGLFLYIRVEDYSDWITSKTRKTSSPLSSFHHWENPSPLPSYSSRDLVTQRKQAGLSQLVWPRLSFQEQERSTVHSTRSLPDNGTQMSLDFREKGRREPGRSEMAIQPTYYDYYGGEFMASAPSSGQNRLHQPQEITLFFLCARFLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7V7</accession>
    <name>A0A4W2E7V7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZDHHC1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E7V7"/>
    <dbReference type="STRING" id="30522.A0A4W2E7V7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018560.1">
        <property type="protein sequence ID" value="ENSBIXP00000032884.1"/>
        <property type="gene ID" value="ENSBIXG00000015579.1"/>
    </dbReference>
    <dbReference type="OMA" id="FCFHIYL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF8">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="132"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="482" mass="53073" checksum="CFDA96F9F6C6E110" modified="2019-09-18" version="1">MYKMNICNKPSNKTAPEKNVWTAPAQASGPSPELQGQRSRRNGWSWPPHPLQIVAWLLYLFFAVIGFGVLVPLLPHHWVPAGYACMGAIFAGHLVVHLTAVSIDPADANVRDKSYAGPLPIFNRSQHAHVIEDLHCNLCDVDVSARSKHCSACNKCVCGFDHHCKWLNNCVGERNYRLFLHSVASALLGVLLLVLVATYVFVEFFVNPMRLRTNRHFEVLKNHTDVWFVFLPAAPVETQAPAILALAALLILLGLLSTALLGHLLCFHIYLMWHKLTTYEYIVQHRPPQEAKGAHRELESCPPKMRPIQEMELYMRTFSHVRPEPPGQARPAAVNANPSRFLATRGQVEPPPPSSPETLALPPRIQPQKKRKRRMYKVPTSGTLNREPQLPRLPGPRTPGRRSSSSSDSADASPAEAYHSASGESLDEIPVAQTRLGSAALAAPVGRPREPGLALQARAPAVFVSPSSGEPRARGGPEADLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8U7</accession>
    <name>A0A4W2E8U7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dickkopf-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DKK3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with LRP5 and LRP6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E8U7"/>
    <dbReference type="STRING" id="30522.A0A4W2E8U7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024532.1">
        <property type="protein sequence ID" value="ENSBIXP00000035463.1"/>
        <property type="gene ID" value="ENSBIXG00000018740.1"/>
    </dbReference>
    <dbReference type="OMA" id="HCQPHGR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23274">
        <property type="entry name" value="Dkk3_Cys2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23014">
        <property type="entry name" value="Dkk3_N_Cys1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000003">
        <property type="entry name" value="Dickkopf WNT-signaling pathway inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047300">
        <property type="entry name" value="Dkk3_Cys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047301">
        <property type="entry name" value="Dkk3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF8">
        <property type="entry name" value="DICKKOPF-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="15">
        <location>
            <begin position="255"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="149"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="458" mass="49016" checksum="056A01D641CDB6BC" modified="2019-09-18" version="1">MPGGNGMRRRGQLPAGPCAVAEASLGWEVAAPGPANGGGLGRSPSLGAGTGGASAAGSPGYQRCRIPAELLAPAVRPPSSSSSAASRQTRARPEVQTRAQRSPVGAAAEMRRLGGTLLYLLLAAAVPTAPAPAPTVTSASIEPGPALSYPQEEATLNEMFREVEELMEDTQYKLRSAVEEMEAEEAAAKTSSEVTLAHLPPSYHNETNTETKVGNNTIHVHREIHKITNNRTGQMVFSETVITSMGDEEGKRSHECIIDEDCGPSRYCQFSSFEYSCQPCRDQQTLCTRDSECCGDQLCVWGRCTKTSTAGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGGRDEDGESLVPREAPDEYEDGGFMEEVRRELENLERSLSVEMALGEPGAASELLEGEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBU4</accession>
    <name>A0A4W2EBU4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase-like oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBU4"/>
    <dbReference type="STRING" id="30522.A0A4W2EBU4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028036.1">
        <property type="protein sequence ID" value="ENSBIXP00000036958.1"/>
        <property type="gene ID" value="ENSBIXG00000020463.1"/>
    </dbReference>
    <dbReference type="OMA" id="KRPVQMH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000178">
        <property type="entry name" value="Saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="4">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="483" mass="53445" checksum="34F8EE98ACAD1745" modified="2019-09-18" version="1">MATQQRPFHLVVFGASGFTGQFVTEEVAREQVSPERTSHLPWAVAGRSREKLLRVLERAAMKLGRPTLSSEVGIIICDITNPASLDEMAKQATVVLNCVGPYRFYGEPVIKACIENGTSCIDISGEPQFLELMYWKYHEKAAEKGVYIIGSSGFDSIPADLGVIYTRNKMNGTLTAVESFLTISSGPEGLCVHDGTWKSAVYGFGDKSNLKKLRNESDMKPVPIVGPKLKRRWPISYCRELNSYSIPFLGADVSVVKRTQRYLHENLEQSPVQYAAYINVGGITSVIKLMFAGLFFLFFVRFGIGRQLLIKFPWLFSFGYFSKQGPTQKQIDASSFTMTFFGQGFSQGVSPVKNKPNIRICTQVKGPEAGYVSTSIAMVQAAMILLNDASDLPKAGGVFTPGAAFSRTKLIDRLNEHGIEFSVISSFVPDLLSPVQRWTHTQALGLSQLEQYYQRTGQKLASNGRNGSLSLPSNLRFHCVSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EC60</accession>
    <name>A0A4W2EC60_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">F-box protein 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FBXO46</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <taxon>Bos</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="13">Choline transporter that plays a role in the choline-acetylcholine system and is required to the efferent innervation of hair cells in the olivocochlear bundle for the maintenance of physiological function of outer hair cells and the protection of hair cells from acoustic injury. Also described as a thiamine pyrophosphate transporter in colon, may mediate the absorption of microbiota-generated thiamine pyrophosphate and contribute to host thiamine (vitamin B1) homeostasis.</text>
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            <text>thiamine diphosphate(out) = thiamine diphosphate(in)</text>
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            <dbReference type="ChEBI" id="CHEBI:58937"/>
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        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the CTL (choline transporter-like) family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Choline_transpo"/>
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        <location>
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            <end position="56"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="226"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368066"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="706" mass="78882" checksum="62222C287EFCF8A5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EG10</accession>
    <name>A0A4W2EG10_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EG10"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026283.1">
        <property type="protein sequence ID" value="ENSBIXP00000036213.1"/>
        <property type="gene ID" value="ENSBIXG00000019628.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="412" mass="47384" checksum="481D79FACED5F28B" modified="2019-09-18" version="1">MLKILQARLQQYVNRELPEVQAGFRKGRGTRDQIAIILWIMEKAREFQKSIYFCLIDYAKAFDCVDHNKLRKILQEMEIPDHLICLLRNLYAGQEATVRTGHGTTDWCQIGKGVHQGCILSSCLFNFYAEYIMRNAGLEETQAGLKIAGRNISNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKMKIMASSPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRCLLLGRKVMTNQDSIFKSRDITLPTKVRLIKAMVFPVVMYGCESWTVKKAERQRIDAFELWCWRRLLRLPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGNLMQRVDSLEKTVMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVMDREAWRAAIHGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJF0</accession>
    <name>A0A4W2EJF0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1 regulatory subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RNA polymerase II subunit 5-mediating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with POLR2E/RPB5, RUVBL2 and RUVBL1. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts with UXT. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts (via the middle C-terminal region) with GTF2F1 and GTF2F2. Interacts with DMAP1. Interacts with TSC1 and TSC2. Interacts with PRPF8 and EFTUD2 in a ZNHIT2-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJF0"/>
    <dbReference type="STRING" id="30522.A0A4W2EJF0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044716.1">
        <property type="protein sequence ID" value="ENSBIXP00000033307.1"/>
        <property type="gene ID" value="ENSBIXG00000016066.1"/>
    </dbReference>
    <dbReference type="OMA" id="HWKKVDD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23159">
        <property type="entry name" value="Prefoldin_URI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000008">
        <property type="entry name" value="unconventional prefoldin RPB5 interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="284"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="335"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="524" mass="59038" checksum="1D0E60C12428DF7D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELK4</accession>
    <name>A0A4W2ELK4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">AKT3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027420516.1">
        <property type="nucleotide sequence ID" value="XM_027564715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELK4"/>
    <dbReference type="SMR" id="A0A4W2ELK4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027822.1">
        <property type="protein sequence ID" value="ENSBIXP00000036817.1"/>
        <property type="gene ID" value="ENSBIXG00000020316.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051438.1">
        <property type="protein sequence ID" value="ENSBIXP00005022559.1"/>
        <property type="gene ID" value="ENSBIXG00005025439.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906492"/>
    <dbReference type="CTD" id="10000"/>
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        <property type="component" value="Chromosome 16"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="148"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="406"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="187"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="479" mass="55775" checksum="F08BDDE6502E78FB" modified="2019-09-18" version="1">MSDVTIVKEGWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDNERRPHFPQFSYSASGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMB2</accession>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <dbReference type="Ensembl" id="ENSBIXP00000037965.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="96" mass="10901" checksum="694377350F4766F0" modified="2019-09-18" version="1">MSNITLNFFFFFFKKHLPNQLRCCPGPFCSHPSLLSRPRAPPPQCSVLYACALCSCILNKSEINVFDAEPQTALASLSDLGYQYKLALWDRLKLGW</sequence>
</entry>
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    <accession>A0A4W2ENZ1</accession>
    <name>A0A4W2ENZ1_BOBOX</name>
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            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="RIBONUCLEASE H"/>
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    </dbReference>
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        <property type="entry name" value="RT_POL"/>
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    </dbReference>
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        <location>
            <begin position="181"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="565" mass="65550" checksum="41858BAFAD5E7528" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPN0</accession>
    <name>A0A4W2EPN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPN0"/>
    <dbReference type="SMR" id="A0A4W2EPN0"/>
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    <sequence length="126" mass="13907" checksum="0AA78FA837E7180A" modified="2019-09-18" version="1">MPERAKSAPAPKKGSKKAVTKAQKKDGKKLKHSRKESYSVYMYKVLKQVHPDTGISSKAMGIMNSFVNDIFECTAGEASRLAHYNKRLTITSREIQTAVHLLLPGELAKHAVSESTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQR9</accession>
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            <taxon>Artiodactyla</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <sequence length="506" mass="57846" checksum="DC35F33E26B3B45B" modified="2019-09-18" version="1">MCIEILCPFFKLGYVSFKKTWQEYTEELYKKDLHDPDNHDGVITDLEPDILECEAKWALESITMNKASGGDRIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISPASKVMLKILQARLQQYVNRELPNVQASFRKGRGTRDQIANICWIMEKAREFQKNIYVCFIDCAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQSCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITADGNCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVCLVKAMVFPVVMYGCESWTVKKAEGRRIDAFELWCWRRHLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRVDSLEKTLMLGGIGAGGEGDDRG</sequence>
</entry>
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    <accession>A0A4W2ES74</accession>
    <name>A0A4W2ES74_BOBOX</name>
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        <submittedName>
            <fullName evidence="9">Actinin alpha 1</fullName>
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        <name evidence="9" type="primary">ACTN1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Artiodactyla</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Spectrin_repeat"/>
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        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="439" mass="45894" checksum="580C20F7F728F447" modified="2019-09-18" version="1">MRHLACPTEAFSELKQLSGEAGLTNGYCCLEGCVHARVGIALPFWWWWWWSWFSCSVTSDSATPWTVATRLLCLWGLVMRKHRLRELISYPLSDSSGKVLVFCVDGGGVFGGHGWRRWRGLGVRTPVCLHGCSLLLSTLLSPQSAAAAPSPVLGNIPPNDGMPGGPIPPGFFQGPPGSQPSPHAQPPPHNPSSMMGPHSQPFMSPRYAGGPRPPIRMGNQPPGGVPGTQPLLPNSMDPTRQQGHPNMGGSMQRMNPPRGMGPMGPGPQNYGSGMRPPPNSLGPAMPGINMGPGAGRPWPNPNSANSIPYSSSSPGTYVGPPGGGGPPGTPIMPSPADSTNSSDNIYTMINPVPPGGSRSNFPMGPGSDGPMGGMGGMEPHHMNGSLGSGDMDGLPKNSPNNISGISNPPGTPRDDGELGGNFLHSFQNDNYSPSMTMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F512</accession>
    <name>A0A4W2F512_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Intraflagellar transport protein 57 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT57 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F512"/>
    <dbReference type="STRING" id="30522.A0A4W2F512"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043355.1">
        <property type="protein sequence ID" value="ENSBIXP00000044163.1"/>
        <property type="gene ID" value="ENSBIXG00000028367.1"/>
    </dbReference>
    <dbReference type="OMA" id="VHAHDQD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019530">
        <property type="entry name" value="Intra-flagellar_transport_57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011">
        <property type="entry name" value="IFT57/HIPPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 57 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10498">
        <property type="entry name" value="IFT57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="308"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="429" mass="48961" checksum="CE7FCCB6A0787F02" modified="2019-09-18" version="1">MAAAAAVVTPSGLEDEVSRSRGEGAGEMVVERGPGAAYHMFVVMEDLVEKLKLLRYEEDLLRKNNLKPPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNAIISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCLAEEALKYIGFTWKRPAYPVEELEEETVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYRLDMNESAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHKSGIESALKETKGFLDRLHNEISRTLEKIGSREKYINNQLEHLVQEYRAAQAQLSEARERYQQGNGGVTERTRILSEVTEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVQMDIRIGVVEHTLLQSKLKEKSNMTRDMHATIIPESAIGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FDP4</accession>
    <name>A0A4W2FDP4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sorting nexin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SNX4</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the regulation of endocytosis and in several stages of intracellular trafficking. Plays a role in recycling endocytosed transferrin receptor and prevent its degradation. Involved in autophagosome assembly by regulating trafficking and recycling of phospholipid scramblase ATG9A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer; heterodimerizes with SNX7 or SNX30. Interacts with WWC1/KIBRA. Identified in a complex with WWC1/KIBRA and dynein components DYNLL1 and DYNC1I2. Interacts with BIN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027392410.1">
        <property type="nucleotide sequence ID" value="XM_027536609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FDP4"/>
    <dbReference type="SMR" id="A0A4W2FDP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032250.1">
        <property type="protein sequence ID" value="ENSBIXP00000018924.1"/>
        <property type="gene ID" value="ENSBIXG00000022652.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011043.1">
        <property type="protein sequence ID" value="ENSBIXP00005003051.1"/>
        <property type="gene ID" value="ENSBIXG00005009284.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889637"/>
    <dbReference type="CTD" id="8723"/>
    <dbReference type="GeneTree" id="ENSGT00930000151029"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07622">
        <property type="entry name" value="BAR_SNX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06864">
        <property type="entry name" value="PX_SNX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000035">
        <property type="entry name" value="Sorting nexin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000031">
        <property type="entry name" value="Sorting nexin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034902">
        <property type="entry name" value="PX_SNX4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034783">
        <property type="entry name" value="SNX4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037430">
        <property type="entry name" value="SNX4_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46596">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46596:SF1">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="61"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="450" mass="52194" checksum="C02B701A1EE9E606" modified="2019-09-18" version="1">MEQAAPDPERLWQPAPLEPLSHPDAGLESMVGEETKGARDEGPGDGTMTENNFSLKKIEISVSEAEKRTGRNAMNMQETYTAYLIETRSVEHNDGQSVLTDSLWRRYSEFELLRNYLLVYYPHIVVPPLPEKRAEFVWHKLSADNMDPDFVERRRIGLENFLLRVASHPILCRDKIFYLFLTQEGNWKETVNETGFQLKADSRLKALNATFRVKNPDKRFIELKHYSDELQSVISHLLRVRARVADRLYGVYKVHGNYGRVFSEWSAIEKEMGDGLQSAGHHMDVYASSIDDILEDEEHYADQLKEYLFYAEALRAVCRKHELMQYDLEMAAQDLASKKQQCEELATGTVRTFSLKGMTTKLFGQETPEQREARIKMLEEQIKEGEQQLKSKNLEGREFVRNAWADIERFKEQKNHDLKEALISYAVMQISMCKKGIQVWTNAKECFSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FI14</accession>
    <name>A0A4W2FI14_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar fusion protein MON1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MON1A</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an important role in membrane trafficking through the secretory apparatus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MON1/SAND family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FI14"/>
    <dbReference type="STRING" id="30522.A0A4W2FI14"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007955.1">
        <property type="protein sequence ID" value="ENSBIXP00000004373.1"/>
        <property type="gene ID" value="ENSBIXG00000010732.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006824.1">
        <property type="protein sequence ID" value="ENSBIXP00005005199.1"/>
        <property type="gene ID" value="ENSBIXG00005010955.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006665"/>
    <dbReference type="OMA" id="QQPFNAK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF14">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="4">
        <location>
            <begin position="160"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="5">
        <location>
            <begin position="321"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="6">
        <location>
            <begin position="448"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="559" mass="62172" checksum="FA462CA1A19A8651" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FI28</accession>
    <name>A0A4W2FI28_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cystatin 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CST11</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414016.1">
        <property type="nucleotide sequence ID" value="XM_027558215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FI28"/>
    <dbReference type="STRING" id="30522.A0A4W2FI28"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046301.1">
        <property type="protein sequence ID" value="ENSBIXP00000013990.1"/>
        <property type="gene ID" value="ENSBIXG00000018802.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007202.1">
        <property type="protein sequence ID" value="ENSBIXP00005005015.1"/>
        <property type="gene ID" value="ENSBIXG00005010800.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902876"/>
    <dbReference type="CTD" id="140880"/>
    <dbReference type="GeneTree" id="ENSGT00910000144356"/>
    <dbReference type="OMA" id="NCVPQEG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061827">
        <property type="term" value="C:sperm head"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000004">
        <property type="entry name" value="Cystatin C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042930">
        <property type="entry name" value="CST11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47886">
        <property type="entry name" value="CYSTATIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47886:SF1">
        <property type="entry name" value="CYSTATIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611416" evidence="3">
        <location>
            <begin position="22"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="33"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="138" mass="16138" checksum="2DCA3E2C2BF46B6F" modified="2019-09-18" version="1">MMARPWQGPRLLLAIVGALVALSYQSKRKTFILVYEVPVSDPVVVTTTDFLTTDFNKKNKDPYNFRIVRVLKAVKRLTDHLEYQVNLEMRRTTCLKSELTNCSFQEGKLYKKIDCYFSVFVIPWFEKYKVLAQNCTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FS42</accession>
    <name>A0A4W2FS42_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Small nuclear ribonucleoprotein polypeptide A'</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNRPA1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing as component of the spliceosome. Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in the spliceosome B complex. Identified in the spliceosome C complex. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B. Contributes to the binding of stem loop IV of U2 snRNA with SNRPB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376982.1">
        <property type="nucleotide sequence ID" value="XM_027521181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FS42"/>
    <dbReference type="SMR" id="A0A4W2FS42"/>
    <dbReference type="STRING" id="30522.A0A4W2FS42"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001994.1">
        <property type="protein sequence ID" value="ENSBIXP00000007370.1"/>
        <property type="gene ID" value="ENSBIXG00000013331.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000614.1">
        <property type="protein sequence ID" value="ENSBIXP00005008309.1"/>
        <property type="gene ID" value="ENSBIXG00005013369.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879567"/>
    <dbReference type="CTD" id="6627"/>
    <dbReference type="GeneTree" id="ENSGT00940000153289"/>
    <dbReference type="OMA" id="PNYREYM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="11">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="255" mass="28370" checksum="25902F05E8CB7209" modified="2019-09-18" version="1">MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQSGQIPGRERRAGPTDDGEEEMEEDTVANGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G1Y7</accession>
    <name>A0A4W2G1Y7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">small monomeric GTPase</fullName>
            <ecNumber evidence="6">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RAB8A</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Phagosome membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027403895.1">
        <property type="nucleotide sequence ID" value="XM_027548094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G1Y7"/>
    <dbReference type="SMR" id="A0A4W2G1Y7"/>
    <dbReference type="STRING" id="30522.A0A4W2G1Y7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040725.1">
        <property type="protein sequence ID" value="ENSBIXP00000024448.1"/>
        <property type="gene ID" value="ENSBIXG00000026606.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021350.1">
        <property type="protein sequence ID" value="ENSBIXP00005012144.1"/>
        <property type="gene ID" value="ENSBIXG00005016437.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896080"/>
    <dbReference type="CTD" id="4218"/>
    <dbReference type="GeneTree" id="ENSGT00940000157246"/>
    <dbReference type="OMA" id="SKMEQNE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030140">
        <property type="term" value="C:trans-Golgi network transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031489">
        <property type="term" value="F:myosin V binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048210">
        <property type="term" value="P:Golgi vesicle fusion to target membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098876">
        <property type="term" value="P:vesicle-mediated transport to the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000202">
        <property type="entry name" value="ras-related protein Rab-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="207" mass="23684" checksum="AA52DBF5530472C5" modified="2019-09-18" version="1">MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFYTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G4R6</accession>
    <name>A0A4W2G4R6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neurexophilin and PC-esterase domain family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NXPE3</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NXPE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419787.1">
        <property type="nucleotide sequence ID" value="XM_027563986.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027419844.1">
        <property type="nucleotide sequence ID" value="XM_027564043.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027419876.1">
        <property type="nucleotide sequence ID" value="XM_027564075.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027419896.1">
        <property type="nucleotide sequence ID" value="XM_027564095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G4R6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052642.1">
        <property type="protein sequence ID" value="ENSBIXP00000043945.1"/>
        <property type="gene ID" value="ENSBIXG00000005903.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023287.1">
        <property type="protein sequence ID" value="ENSBIXP00005013434.1"/>
        <property type="gene ID" value="ENSBIXG00005017577.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905937"/>
    <dbReference type="CTD" id="91775"/>
    <dbReference type="GeneTree" id="ENSGT00950000182866"/>
    <dbReference type="OMA" id="IVKPRPF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF9">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="2">
        <location>
            <begin position="331"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="559" mass="64042" checksum="49A6A6CF3A37A371" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G7R8</accession>
    <name>A0A4W2G7R8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 526</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF526</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371473.1">
        <property type="nucleotide sequence ID" value="XM_027515672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G7R8"/>
    <dbReference type="SMR" id="A0A4W2G7R8"/>
    <dbReference type="STRING" id="30522.A0A4W2G7R8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021250.1">
        <property type="protein sequence ID" value="ENSBIXP00000011716.1"/>
        <property type="gene ID" value="ENSBIXG00000016921.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024439.1">
        <property type="protein sequence ID" value="ENSBIXP00005014207.1"/>
        <property type="gene ID" value="ENSBIXG00005018214.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876446"/>
    <dbReference type="CTD" id="116115"/>
    <dbReference type="GeneTree" id="ENSGT00940000162624"/>
    <dbReference type="OMA" id="DGPFQCG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002041">
        <property type="entry name" value="Zinc finger protein 526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002659">
        <property type="entry name" value="Zinc finger protein 526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF243">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="387"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="449"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="580"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="223"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="408"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="608"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="613"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="677" mass="73935" checksum="3CBDA098870A6F9A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G881</accession>
    <name>A0A4W2G881_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="57" mass="6573" checksum="D7CC63A656E29958" modified="2019-09-18" version="1">MRWLDGITDSMGVSLGERKMKNLEREKMGGACKYQNTHDCIILPGECKSWKKHCCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIZ3</accession>
    <name>A0A4W2GIZ3_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">EPM2A interacting protein 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">EPM2AIP1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C2H2_12"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="608" mass="69867" checksum="116B969914FB1F06" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GNZ6</accession>
    <name>A0A4W2GNZ6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">poly(A)-specific ribonuclease</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CNOT6L</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027544839.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027400641.1">
        <property type="nucleotide sequence ID" value="XM_027544840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GNZ6"/>
    <dbReference type="SMR" id="A0A4W2GNZ6"/>
    <dbReference type="STRING" id="30522.A0A4W2GNZ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036813.1">
        <property type="protein sequence ID" value="ENSBIXP00000021926.1"/>
        <property type="gene ID" value="ENSBIXG00000024918.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000036814.1">
        <property type="protein sequence ID" value="ENSBIXP00000040777.1"/>
        <property type="gene ID" value="ENSBIXG00000024918.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049884.1">
        <property type="protein sequence ID" value="ENSBIXP00005019656.1"/>
        <property type="gene ID" value="ENSBIXG00005022921.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049889.1">
        <property type="protein sequence ID" value="ENSBIXP00005019663.1"/>
        <property type="gene ID" value="ENSBIXG00005022921.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894426"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10312">
        <property type="entry name" value="Deadenylase_CCR4b"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000008">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034967">
        <property type="entry name" value="Deadenylase_CCR4b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF35">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="16" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="21">
        <location>
            <begin position="193"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="555" mass="63001" checksum="C6E94F1BAE222241" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GUG8</accession>
    <name>A0A4W2GUG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">selenide, water dikinase</fullName>
            <ecNumber evidence="2">2.7.9.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEPHS1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>hydrogenselenide + ATP + H2O = selenophosphate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2GUG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022599.1">
        <property type="protein sequence ID" value="ENSBIXP00000012533.1"/>
        <property type="gene ID" value="ENSBIXG00000017593.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035224.1">
        <property type="protein sequence ID" value="ENSBIXP00005021400.1"/>
        <property type="gene ID" value="ENSBIXG00005024439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000950"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000006">
        <property type="entry name" value="Selenide water dikinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="SELENIDE, WATER DIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256:SF2">
        <property type="entry name" value="SELENIDE, WATER DIKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="11">
        <location>
            <begin position="69"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="321" mass="35449" checksum="1923BF67089DD3DD" modified="2019-09-18" version="1">MSARESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H4X3</accession>
    <name>A0A4W2H4X3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Iron-binding zinc finger CDGSH type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H4X3"/>
    <dbReference type="SMR" id="A0A4W2H4X3"/>
    <dbReference type="STRING" id="30522.A0A4W2H4X3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052661.1">
        <property type="protein sequence ID" value="ENSBIXP00000043959.1"/>
        <property type="gene ID" value="ENSBIXG00000028147.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040096.1">
        <property type="protein sequence ID" value="ENSBIXP00005024735.1"/>
        <property type="gene ID" value="ENSBIXG00005027264.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235809"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052950">
        <property type="entry name" value="CISD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491">
        <property type="entry name" value="CDGSH IRON SULFUR DOMAIN PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491:SF3">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="6">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15343" checksum="BAEB2EC60EAA50C1" modified="2019-09-18" version="1">MGSVCSIGMPVWPAAWTVNQRRGHHLLAGPMVPQNPSQVRGGPKNTHQGGAGGWENLQVVCVWPQQKVVLCDGSHFFKCPGLSPLKFKAQETCMVARCTCKATQKPPYCDGTHKSELVQKAELGSPLWEWLTPGITAPIAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H7U7</accession>
    <name>A0A4W2H7U7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement factor D</fullName>
            <ecNumber evidence="3">3.4.21.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Adipsin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">C3 convertase activator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Properdin factor D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CFD</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.</text>
            <dbReference type="EC" id="3.4.21.46"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.46" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027402479.1">
        <property type="nucleotide sequence ID" value="XM_027546678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H7U7"/>
    <dbReference type="SMR" id="A0A4W2H7U7"/>
    <dbReference type="STRING" id="30522.A0A4W2H7U7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007679.1">
        <property type="protein sequence ID" value="ENSBIXP00000004515.1"/>
        <property type="gene ID" value="ENSBIXG00000010839.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042450.1">
        <property type="protein sequence ID" value="ENSBIXP00005026301.1"/>
        <property type="gene ID" value="ENSBIXG00005002370.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895440"/>
    <dbReference type="CTD" id="1675"/>
    <dbReference type="GeneTree" id="ENSGT00940000162255"/>
    <dbReference type="OMA" id="YTRTAPY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000014">
        <property type="entry name" value="Complement factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF54">
        <property type="entry name" value="COMPLEMENT FACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 16" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611830" description="Complement factor D" evidence="17">
        <location>
            <begin position="22"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="18">
        <location>
            <begin position="27"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="259" mass="27878" checksum="0D56DB06FEC4C2A9" modified="2019-09-18" version="1">MADRSLHLVVLILLGTALCAAQPRGRILRGQEAPSHSRPYMASVQVNGKHVCGGFLIAEQWVMSAAHCLEDVADGKVQVLLGAHSLSQPEPSKRLYDVLRVVPHPGSRTETIDHDLLLLQLSEKAVLGPAVQLLPWQREDRDVAAGTLCDVAGWGVVSHTGRKPDRLQHLLLPVLDRATCNLRTYHDGTITERMMCAESNRRDTCKGDSGGPLVCGSVAEGVVTSGSRICGNHKKPGIYTRLASYVAWIDGVMAEGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H9P0</accession>
    <name>A0A4W2H9P0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HRAS</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027388053.1">
        <property type="nucleotide sequence ID" value="XM_027532252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H9P0"/>
    <dbReference type="SMR" id="A0A4W2H9P0"/>
    <dbReference type="STRING" id="30522.A0A4W2H9P0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012475.1">
        <property type="protein sequence ID" value="ENSBIXP00000027732.1"/>
        <property type="gene ID" value="ENSBIXG00000008654.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042698.1">
        <property type="protein sequence ID" value="ENSBIXP00005026456.1"/>
        <property type="gene ID" value="ENSBIXG00005005350.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886222"/>
    <dbReference type="CTD" id="3265"/>
    <dbReference type="GeneTree" id="ENSGT00940000155653"/>
    <dbReference type="OMA" id="FICIREE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905360">
        <property type="term" value="C:GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160185">
        <property type="term" value="F:phospholipase C activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060612">
        <property type="term" value="P:adipose tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071480">
        <property type="term" value="P:cellular response to gamma radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042832">
        <property type="term" value="P:defense response to protozoan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048144">
        <property type="term" value="P:fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090402">
        <property type="term" value="P:oncogene-induced cell senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050679">
        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048146">
        <property type="term" value="P:positive regulation of fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000630">
        <property type="term" value="P:positive regulation of miRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090314">
        <property type="term" value="P:positive regulation of protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900029">
        <property type="term" value="P:positive regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032729">
        <property type="term" value="P:positive regulation of type II interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090303">
        <property type="term" value="P:positive regulation of wound healing"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048169">
        <property type="term" value="P:regulation of long-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098696">
        <property type="term" value="P:regulation of neurotransmitter receptor localization to postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014044">
        <property type="term" value="P:Schwann cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042088">
        <property type="term" value="P:T-helper 1 type immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000096">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="189" mass="21272" checksum="D3A360BFEC4C225B" modified="2019-09-18" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINHVKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKVRKLSPPDEGGPGCLSCRCLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HAE3</accession>
    <name>A0A4W2HAE3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 92</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM92</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027374742.1">
        <property type="nucleotide sequence ID" value="XM_027518941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HAE3"/>
    <dbReference type="STRING" id="30522.A0A4W2HAE3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004516.1">
        <property type="protein sequence ID" value="ENSBIXP00000006061.1"/>
        <property type="gene ID" value="ENSBIXG00000012198.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011961.1">
        <property type="protein sequence ID" value="ENSBIXP00005029050.1"/>
        <property type="gene ID" value="ENSBIXG00005008911.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878091"/>
    <dbReference type="CTD" id="162461"/>
    <dbReference type="GeneTree" id="ENSGT00390000014943"/>
    <dbReference type="OMA" id="MSQAWVT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31359">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31359:SF31">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="238" mass="26568" checksum="5202C882A730462D" modified="2019-09-18" version="1">MERNCMETETGRNTAKQGGVKQKTDDRTGRDEKQRLGSSRVRQLRPHPEVGPYRWRVVGEVADPPLSSFPYTRKLSGPQARMSDTWVASLLLFGLLAGLQQAAATCGLLTCPKGFKCCGSDCCQEYQLEQYDFFSHPLRTSVIIFLIIILLLCIYGVTKHLYLNCRKSEQEAPTALPEQPPIAPVERVTAPISEPPPPYSEIILKPVLGLPPLEPPPPYSFRPEEYAGFCRGIDNSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HDL0</accession>
    <name>A0A4W2HDL0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Poly(A) binding protein interacting protein 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PAIP2B</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAIP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410739.1">
        <property type="nucleotide sequence ID" value="XM_027554938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HDL0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046291.1">
        <property type="protein sequence ID" value="ENSBIXP00000013970.1"/>
        <property type="gene ID" value="ENSBIXG00000018789.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012010.1">
        <property type="protein sequence ID" value="ENSBIXP00005029035.1"/>
        <property type="gene ID" value="ENSBIXG00005008888.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901056"/>
    <dbReference type="CTD" id="400961"/>
    <dbReference type="GeneTree" id="ENSGT00390000017284"/>
    <dbReference type="OMA" id="EDKEWFI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040396">
        <property type="entry name" value="PAIP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154:SF5">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="124" mass="14368" checksum="C833EAE01E6053BB" modified="2019-09-18" version="1">MMNGSSVANTSPSVKSKEDQVLNGHDEKENPFAEYMWMENEEDFNRQVEEELQEQEFLDRCFQEMLDEEDQDWFIPSRDLPQAMGQLQQQLNGLSVSDGRDSEDILSKSNLNPDAKEFIPGVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HEG7</accession>
    <name>A0A4W2HEG7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">StAR related lipid transfer domain containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">STARD4</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="PROSITE" id="PS50848"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="208" mass="23765" checksum="037D48A02A0A76B9" modified="2019-09-18" version="1">MEALPDAAVLATRLKNTLIQYHNLEDEKWRVAKKTKDVTIWRKPSEEFNGYLFKAQGVIDDLVNSVIDHIRPGPCRLDWDSLMTSLDILEHFEENCCVMRYTTAGQLWNIISPREFVDFSYTVGYKEGLLSCGISLDWSEKRSEFVRGYNHPCGWFCVPLKESPSRSLLTGYIQTDLRGMIPQSAVDTAMASTLINFYGDLQKALQKA</sequence>
</entry>
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    <accession>A0A4W2HNV0</accession>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14725" checksum="1A70AC57894512D3" modified="2019-09-18" version="1">MFFHVSTFWCGGFQFIGKIAISKCLPFRLEPDFSLMIYIKKFFPLRIPSIMSLSGIKCTLKKGAGQLFQRWRKASLDRPPPSPPLTLSPPSFQRARVSTWGTWPAHSLLPKCCMPAWTTNQTAPTPCVFT</sequence>
</entry>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 25"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF9">
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="532" mass="58288" checksum="42434284BBA5AAB0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HR41</accession>
    <name>A0A4W2HR41_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3">Active breakpoint cluster region-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ABR</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with DLG4.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027373844.1">
        <property type="nucleotide sequence ID" value="XM_027518043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HR41"/>
    <dbReference type="SMR" id="A0A4W2HR41"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015070.1">
        <property type="protein sequence ID" value="ENSBIXP00000000964.1"/>
        <property type="gene ID" value="ENSBIXG00000029139.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005033635.1"/>
        <property type="gene ID" value="ENSBIXG00005014752.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153491"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08686">
        <property type="entry name" value="C2_ABR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13366">
        <property type="entry name" value="PH_ABR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04387">
        <property type="entry name" value="RhoGAP_Bcr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000057">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000014">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000112">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000004">
        <property type="entry name" value="active breakpoint cluster region-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037769">
        <property type="entry name" value="Abr/Bcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037865">
        <property type="entry name" value="ABR_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182:SF5">
        <property type="entry name" value="ACTIVE BREAKPOINT CLUSTER REGION-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN BCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="DH" evidence="13">
        <location>
            <begin position="91"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="301"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="484"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="14">
        <location>
            <begin position="647"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="859" mass="97612" checksum="84797AC01471015B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HSG7</accession>
    <name>A0A4W2HSG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Serine/threonine-protein kinase VRK1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Vaccinia-related kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">VRK1</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with HDAC1, KAT2B, SETDB1, KDM3A and KDM4A. Associates with the nucleosome through interactions with nucleosome DNA, histone H2A and histone H2B; the interaction with H2A and H2B is mediated by the nucleosome acidic patch, a cluster of negatively charged residues of H2A and H2B forming a cleft within the nucleosome core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027376878.1">
        <property type="nucleotide sequence ID" value="XM_027521077.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027376879.1">
        <property type="nucleotide sequence ID" value="XM_027521078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HSG7"/>
    <dbReference type="SMR" id="A0A4W2HSG7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016031.1">
        <property type="protein sequence ID" value="ENSBIXP00000008368.1"/>
        <property type="gene ID" value="ENSBIXG00000014227.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046400.1">
        <property type="protein sequence ID" value="ENSBIXP00005035797.1"/>
        <property type="gene ID" value="ENSBIXG00005017132.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879513"/>
    <dbReference type="CTD" id="7443"/>
    <dbReference type="GeneTree" id="ENSGT00940000155554"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14122">
        <property type="entry name" value="STKc_VRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000348">
        <property type="entry name" value="serine/threonine-protein kinase VRK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="37"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="352"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="396" mass="45358" checksum="A30A8F0A7BEC7684" modified="2019-09-18" version="1">MPRVKAAQAGRQGPAKRRLAEHFAAGEIITDMNKKEWKLGSPIGQGGFGCIYLADMNSSKSVGNDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTHKLKYLGVPKYWGSGLHDKNGKSYRFMIIDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLSYKNPDQVYLVDYGLAYRYCPEGTHKEYKEDPKRCHDGTVEFTSIDAHNGVAPSRRGDLEILGYCMIQWLSGHLPWEDNLKDPNYVRDSKIRYRENIASLMDKCFPEKNKPDEIAKYMETVKLLDYVEKPLYQKLRDILLQGLKAIGSKDDGKLDLTVVENGSLKAKPVAKKRKKEAEESVESSVEDMECSDKQTEEATQTRSKTRKRVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I8P2</accession>
    <name>A0A4W2I8P2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine zipper transcription factor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LZTFL1</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates ciliary localization of the BBSome complex. Together with the BBSome complex, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May play a role in neurite outgrowth. May have tumor suppressor function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Self-associates. Interacts with BBS9; the interaction mediates the association of LZTL1 with the BBsome complex and regulates BBSome ciliary trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LZTFL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027378645.1">
        <property type="nucleotide sequence ID" value="XM_027522844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I8P2"/>
    <dbReference type="SMR" id="A0A4W2I8P2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010252.1">
        <property type="protein sequence ID" value="ENSBIXP00000003242.1"/>
        <property type="gene ID" value="ENSBIXG00000009640.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032700.1">
        <property type="protein sequence ID" value="ENSBIXP00005040286.1"/>
        <property type="gene ID" value="ENSBIXG00005022882.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880553"/>
    <dbReference type="CTD" id="54585"/>
    <dbReference type="GeneTree" id="ENSGT00390000016415"/>
    <dbReference type="OMA" id="QMEGTTA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002177">
        <property type="term" value="C:manchette"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903568">
        <property type="term" value="P:negative regulation of protein localization to ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026157">
        <property type="entry name" value="LZTFL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635:SF0">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15294">
        <property type="entry name" value="Leu_zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="147"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="299" mass="34577" checksum="7BBDE61330227554" modified="2019-09-18" version="1">MAELGLNEHHQNEVINYMRFARSKRGLRLKTVDSCFQDLKESRLVEETFTVDEVSEVLNGLQAVVHSEVESELINTAYTNVLLLRQLFSQAEKWYLKLQTDISELENRELLEQVAEFEKAEFTSSNKKSIIDSMKPKLAPLHEGGAAELLNKEIIRLQEENEKLKSRLKTIESQATDALDEKSKLERALQDLQLEHGSQKDFIKAQDLSDLENTVAALKSEFQKTLNDQTENQKSLEENLATAKHDLLRVQEQLSMAEKELEKKFQQTAAFRNMKEILTKKNDQIKDLRKRLAKYEPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IK90</accession>
    <name>A0A4W2IK90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear factor of activated T-cells, cytoplasmic 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">NFAT transcription complex cytosolic component</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NFATC4</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with HOMER2 and HOMER3; this interaction may compete with calcineurin/PPP3CA-binding and hence prevent NFATC1 dephosphorylation and activation. Interacts with TLE6/GRG6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2IK90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034439.1">
        <property type="protein sequence ID" value="ENSBIXP00000020059.1"/>
        <property type="gene ID" value="ENSBIXG00000002653.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042135.1">
        <property type="protein sequence ID" value="ENSBIXP00005044647.1"/>
        <property type="gene ID" value="ENSBIXG00005004941.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160923"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01178">
        <property type="entry name" value="IPT_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07881">
        <property type="entry name" value="RHD-n_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF11">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="15">
        <location>
            <begin position="401"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="15"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="208"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="771"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="272"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="776"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="805"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="900" mass="95308" checksum="10B850E335E6EBE0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IP54</accession>
    <name>A0A4W2IP54_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleiotropic regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PLRG1</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in pre-mRNA splicing as component of the spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Component of the minor spliceosome, which splices U12-type introns. Within this complex, interacts with CRIPT. Also interacts directly in the complex with BCAS2 and PRPF19. Interacts with USB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PRL1/PRL2 family.</text>
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        <property type="nucleotide sequence ID" value="XM_027513499.1"/>
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    <dbReference type="SMR" id="A0A4W2IP54"/>
    <dbReference type="STRING" id="30522.A0A4W2IP54"/>
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        <property type="protein sequence ID" value="ENSBIXP00000030497.1"/>
        <property type="gene ID" value="ENSBIXG00000012501.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040852.1">
        <property type="protein sequence ID" value="ENSBIXP00005043976.1"/>
        <property type="gene ID" value="ENSBIXG00005027740.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875083"/>
    <dbReference type="CTD" id="5356"/>
    <dbReference type="GeneTree" id="ENSGT00940000155316"/>
    <dbReference type="OMA" id="FAMCFDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900087">
        <property type="term" value="P:positive regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000012">
        <property type="entry name" value="Putative pleiotropic regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="GPROTEINBRPT"/>
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
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        <location>
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            <end position="240"/>
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    <feature type="repeat" description="WD" evidence="7">
        <location>
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        </location>
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    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="283"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="408"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="513" mass="56789" checksum="96423FAA92BCC0E8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSV0</accession>
    <name>A0A4W2BSV0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010468.1">
        <property type="protein sequence ID" value="ENSBIXP00000003135.1"/>
        <property type="gene ID" value="ENSBIXG00000009531.1"/>
    </dbReference>
    <dbReference type="OMA" id="SCIGTHA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="704" mass="83236" checksum="5FF34B252E9F397A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSV3</accession>
    <name>A0A4W2BSV3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 6B.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSV3"/>
    <dbReference type="SMR" id="A0A4W2BSV3"/>
    <dbReference type="STRING" id="30522.A0A4W2BSV3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010369.1">
        <property type="protein sequence ID" value="ENSBIXP00000003184.1"/>
        <property type="gene ID" value="ENSBIXG00000009572.1"/>
    </dbReference>
    <dbReference type="OMA" id="WDGQRDD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000278">
        <property type="entry name" value="Cyt_c_oxidase_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="86" mass="10169" checksum="89362CBFF1B3F471" modified="2019-09-18" version="1">MAEYIKTKIKNYQSAPFDSRFPNQNQTRNCWQNYLDFHRCEKAMTAKGGDVSVCEWYRRVYKSLCSTSWVSTWDNRQAEGTFPGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUD4</accession>
    <name>A0A4W2BUD4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Germ cell-specific gene 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GSG1L</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383410.1">
        <property type="nucleotide sequence ID" value="XM_027527609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUD4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011630.1">
        <property type="protein sequence ID" value="ENSBIXP00000002584.1"/>
        <property type="gene ID" value="ENSBIXG00000009054.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030440.1">
        <property type="protein sequence ID" value="ENSBIXP00005018190.1"/>
        <property type="gene ID" value="ENSBIXG00005021641.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883695"/>
    <dbReference type="CTD" id="146395"/>
    <dbReference type="GeneTree" id="ENSGT01050000244814"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000005">
        <property type="entry name" value="Germ cell-specific gene 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012478">
        <property type="entry name" value="GSG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF35">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07803">
        <property type="entry name" value="GSG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610287" description="Germ cell-specific gene 1-like protein" evidence="11">
        <location>
            <begin position="27"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="330" mass="36621" checksum="370721111D4A86B9" modified="2019-09-18" version="1">MKTSRRGRALLAVALNLLALLFATTAFLTTHWCQGTQRVPKPGCGQGGSANCPNSGANATANGTAAPAVPAAPAAGNGPPGGALYSWETGDDRFLFRNFHTGIWYSCEEELGGLGEKCRSFIDLAPASEKGVLWLSVVSEVLYILLLVVGFSLMCLELFHSSNVIDGLKLNAFAAVFTVLSGLLGMVAHMMYTQVFQVTVSLGPEDWRPHSWDYGWSFCLAWGSFTCCMAASVTTLNSYTKTVIEFRHKRKVFEQGYREEPTFIDPEAVKYFRERMEKGDGHEEEDFRLDCRHERYPARHQPHMGDSWPRSSAHEAAELNRQCWVLGHWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BX29</accession>
    <name>A0A4W2BX29_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neuronal integral membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BX29"/>
    <dbReference type="STRING" id="30522.A0A4W2BX29"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006920.1">
        <property type="protein sequence ID" value="ENSBIXP00000004865.1"/>
        <property type="gene ID" value="ENSBIXG00000006826.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037670">
        <property type="entry name" value="C11orf87"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31870:SF2">
        <property type="entry name" value="CHROMOSOME 11 OPEN READING FRAME 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31870">
        <property type="entry name" value="SI:DKEY-183I3.9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="286"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="41485" checksum="1DE63B04D52DF97A" modified="2019-09-18" version="1">MVHLHCLILNKCSRLYFRFCHICVQKCASHGRSFCSEPGQHRQDWRKGRSSSAERGNLLCALRGEGSLECKNVCLGPRGQEVRVKNRAARERSFNPSQAERCGSLVLWQRKRARGHAFEAGPERISWAPLAPWPVCLPTVAPLVLRSRAHPLPRQGVVLGAAGSVLFLAGENRVLKRGSLEGPAPPSPAPMSARAPKELRLALPPCLLNRTFASPNASGSGNASARGQGAGGGSSGTCITQVGQQLFQSFSSTLVLIVLVTLIFCLIVLSLSTFHIHKRRMKKRKMQRAQEEYERDHCGGNRGGRGLPQPEGGQASTQGKEARLERQARDSAFCAPSSASSSSSPPGLPCQAPCAPPPPPAPSPQGAHTASSCLDPVGEGLLQAVVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BXX2</accession>
    <name>A0A4W2BXX2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BXX2"/>
    <dbReference type="STRING" id="30522.A0A4W2BXX2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008788.1">
        <property type="protein sequence ID" value="ENSBIXP00000003978.1"/>
        <property type="gene ID" value="ENSBIXG00000006527.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="91" mass="9317" checksum="E9AD6FF7A61E4842" modified="2019-09-18" version="1">MREDPGSIPGSGRSSGEGNAGATWAAGSTVQQTKSASWGVRGTGLTTSGGTQGASAILPRRLTGARSWPCSSTGYESSVFTLHIWGKYTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C379</accession>
    <name>A0A4W2C379_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C379"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002339.1">
        <property type="protein sequence ID" value="ENSBIXP00000007178.1"/>
        <property type="gene ID" value="ENSBIXG00000001294.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="182"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="405" mass="47161" checksum="C3854D35BE71CB88" modified="2019-09-18" version="1">MEKKCKRAKWLSEEALQIAVKRREAKSKGEKERYKHLNAEFQRIARRDKKAFFSDHCKEIEENNRMGKTRDLFKKISDSKGTFHAKMSSIKDRNGMDLTEAEDMKKRWQEYTEELYKKDLHNPDNHDGVITDLEPDILECEVKWALGSITMNKASGGDRIPVELFQILKDDAVKVLHSICQQIWKTQQWPQGWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQNMNRELPDVHAGFRKGRRTRDQTANIHWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGRTDEFQIGKGVRQGCILSPCLFNLYAEYIMRNTGLEEAQAGIKTAGRNINNLRYADDTTFMAESEEELKSLLMKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C663</accession>
    <name>A0A4W2C663_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sex hormone-binding globulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SHBG</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C663"/>
    <dbReference type="SMR" id="A0A4W2C663"/>
    <dbReference type="STRING" id="30522.A0A4W2C663"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015856.1">
        <property type="protein sequence ID" value="ENSBIXP00000008258.1"/>
        <property type="gene ID" value="ENSBIXG00000014140.1"/>
    </dbReference>
    <dbReference type="OMA" id="TEPWAFS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000107">
        <property type="entry name" value="Sex hormone-binding globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051145">
        <property type="entry name" value="GAS-SHBG-PROS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040">
        <property type="entry name" value="LAMININ G-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040:SF3">
        <property type="entry name" value="SEX HORMONE-BINDING GLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0754">Steroid-binding</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021357281" description="Sex hormone-binding globulin" evidence="14">
        <location>
            <begin position="26"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="44"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="401" mass="43316" checksum="C8A2C53B25BD86E3" modified="2019-09-18" version="1">MVSRGPLAIWHGLLLLLLLPLPSHGGPALRPPLPSQTTEDSPALHLSSGPGQEPVTIMTFNLTKITKISSSFEFRTWDPEGVIFYGDTNPKNDWFMLGLRDGRPEIQLHNHWAQLTVSAGPRLDDGKWHQMEVKIHGDSLLLRVDGVEVLCLRQVFGQQANNSQLIMRIALGGLLFPASDLRLPLVPALDACLRQDDWLDQQAQTSASVPTSVRSCAVESQPGIFFPPGTGAEFGLQEIPRPHAEPWAFSLDLALQLAAGSGRLLALGTPENPSWLSIHLQDQKVVLSSGSGPGLDLPLILGLRLQLNLTVSGVVLSQGAKKEILALPPTGPGSLLDLWVQPHGRLFLGALPGEATSASFCLDGLWAQGRSLDMDRAQSRSLNIWTHSCPQNPGNGSDTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6G3</accession>
    <name>A0A4W2C6G3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coiled-coil domain containing 157</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSBIXG00000001067.1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43696">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43696:SF9">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 157"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9216" checksum="D5632488D7F495E7" modified="2019-09-18" version="1">MAHLLGSPACMDSLRKDLTDLQGAIVDVFSRAGPVRFPSWKFPDRAACDLDMVALLEHYDHVPGDPEFTQLAHAVLLELVIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6W4</accession>
    <name>A0A4W2C6W4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">OSBP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2 8">Belongs to the OSBP family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Oxysterol-binding protein"/>
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    </dbReference>
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        <location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007318.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="916" mass="101790" checksum="0B6FA278527C8BBD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7M5</accession>
    <name>A0A4W2C7M5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="9">Chromogranin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CHGA</name>
    </gene>
    <organism evidence="12 13">
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="7">Strongly inhibits glucose induced insulin release from the pancreas.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="8">Secretory vesicle</location>
            <location evidence="8">Neuronal dense core vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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    <dbReference type="AlphaFoldDB" id="A0A4W2C7M5"/>
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        <property type="term" value="P:negative regulation of catecholamine secretion"/>
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        <property type="term" value="P:negative regulation of insulin secretion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Chromogranin_CS"/>
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        <property type="entry name" value="Granin"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10583:SF1">
        <property type="entry name" value="CHROMOGRANIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Granin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00659">
        <property type="entry name" value="CHROMOGRANIN"/>
    </dbReference>
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        <property type="entry name" value="GRANINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GRANINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021393305" description="Chromogranin-A" evidence="11">
        <location>
            <begin position="19"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="299" mass="33862" checksum="22FAA31CEF9D9FFA" modified="2019-09-18" version="1">MRSAAVLALLLCAGQVIALPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSKECFETLRGDERILSILRHQNLLKELQDLALQGAKERTHQQKKHSSYEDELSEVLEKPNDQAEPKGWPEDGAGKMGAEEAKPPEGKGEWAHSRQEEEEMARAPQVLFRGGKSGEPEQEEQLSKEWEDAKRWSKMDQLAKELTAEKRLEGEEEEEEDPDRSMRLSFRARGYGFRGPGLQLRRGWRPNSREDSVEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLEELRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9V6</accession>
    <name>A0A4W2C9V6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C9V6"/>
    <dbReference type="STRING" id="30522.A0A4W2C9V6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018058.1">
        <property type="protein sequence ID" value="ENSBIXP00000009660.1"/>
        <property type="gene ID" value="ENSBIXG00000006497.1"/>
    </dbReference>
    <dbReference type="OMA" id="WMWIDEH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF6">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="91"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="205" mass="23774" checksum="3D1FDA98B61D17AF" modified="2019-09-18" version="1">MKTFKEMENEDGYMTLKIKNRFEPRKKSKDFALYLQCYCLMLVLGCIGILIFMMAVIGLNFWNREMDFLQKANITSLAGNNYVCPSDWLLNQGKCYRFSTFSKTWNESQRDCLKLQAHLPVIQNSKELKFIQKSLKSGQPSWLGLCITSPGKQWVWINEHLFVEQEIFSVIGPIDNMSCAVITRNQVYSEDCDSKFNGICERDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAE6</accession>
    <name>A0A4W2CAE6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ubiquitin-associated and SH3 domain-containing protein B</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cbl-interacting protein p70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Suppressor of T-cell receptor signaling 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">T-cell ubiquitin ligand 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Tyrosine-protein phosphatase STS1/TULA2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">UBASH3B</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with JAK2 (in vitro). Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins. Interacts with ZAP70 (ubiquitinated form).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027419253.1">
        <property type="nucleotide sequence ID" value="XM_027563452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAE6"/>
    <dbReference type="SMR" id="A0A4W2CAE6"/>
    <dbReference type="STRING" id="30522.A0A4W2CAE6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010205.1">
        <property type="protein sequence ID" value="ENSBIXP00000003257.1"/>
        <property type="gene ID" value="ENSBIXG00000009667.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005958.1">
        <property type="protein sequence ID" value="ENSBIXP00005005648.1"/>
        <property type="gene ID" value="ENSBIXG00005011282.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905736"/>
    <dbReference type="CTD" id="84959"/>
    <dbReference type="GeneTree" id="ENSGT00940000156097"/>
    <dbReference type="OMA" id="NMPKEIP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038063">
        <property type="term" value="P:collagen-activated tyrosine kinase receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090331">
        <property type="term" value="P:negative regulation of platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11936">
        <property type="entry name" value="SH3_UBASH3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14301">
        <property type="entry name" value="UBA_UBS3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000008">
        <property type="entry name" value="Ubiquitin associated and SH3 domain containing B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000051">
        <property type="entry name" value="ubiquitin-associated and SH3 domain-containing protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000052">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000001">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035632">
        <property type="entry name" value="UBASH3B_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF29">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="319"/>
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            <position position="576"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054227"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00079820"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00080224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081839"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003257.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="649" mass="72691" checksum="E30BAAC79673370D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <name evidence="9" type="primary">DPY19L3</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="716" mass="83142" checksum="031DAE6C0C2E81B7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEC9</accession>
    <name>A0A4W2CEC9_BOBOX</name>
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        <submittedName>
            <fullName evidence="12">Transmembrane p24 trafficking protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TMED6</name>
    </gene>
    <organism evidence="12 13">
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        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEC9"/>
    <dbReference type="SMR" id="A0A4W2CEC9"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000019059.1">
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        <property type="gene ID" value="ENSBIXG00000000880.1"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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        </location>
    </feature>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="240" mass="27850" checksum="66F038D66E2E1872" modified="2019-09-18" version="1">MFPLLFVAGLVVLNLVSSARSQKTEPLSGTGDQSLFRGADQHDFAIVIPPGGTECFWQFAHQTGYFYFSYEVQRTIGMSHDRHVAATAHTPQGFLIETSKNVRGQINFSTHETGFYQLCLANQQNRFASVQVYLNFGVFYEGPEMDHKENERKQLNDTLDAIEESTRKVHYNIFHMWRHYNFARMRKTADFFLLQSNYNYVNWWSTAQSLVIVLSGILQLYFLKRLFNTPMTTETQKPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CF92</accession>
    <name>A0A4W2CF92_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="49"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="167" mass="17476" checksum="9903429A800E1BCC" modified="2019-09-18" version="1">MDRGGPAGSPLISSSEPAPPVVAVRDLSPGRTGPGPSGPGGGARSGGGRPAAANAARERSRVQTLRHAFLELQRTLPSVPPDTKLSKLDVLLLATTYIAHLTRSLQDDAEAPADPGLGALRGDGYLHPVKKWPMRSRLYIGATGQFLKHPVSGEKTNHDNIPTDSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKA0</accession>
    <name>A0A4W2CKA0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 68</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PPP1R37</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with PPP1CA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP1R37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371296.1">
        <property type="nucleotide sequence ID" value="XM_027515495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CKA0"/>
    <dbReference type="SMR" id="A0A4W2CKA0"/>
    <dbReference type="STRING" id="30522.A0A4W2CKA0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022077.1">
        <property type="protein sequence ID" value="ENSBIXP00000012203.1"/>
        <property type="gene ID" value="ENSBIXG00000017289.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049127.1">
        <property type="protein sequence ID" value="ENSBIXP00005018219.1"/>
        <property type="gene ID" value="ENSBIXG00005021626.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876360"/>
    <dbReference type="CTD" id="284352"/>
    <dbReference type="GeneTree" id="ENSGT00940000157454"/>
    <dbReference type="OMA" id="EHELRCP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000324">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF9">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="467"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="588"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="699" mass="75697" checksum="B3C19171B2DFF3C1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CL38</accession>
    <name>A0A4W2CL38_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CL38"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022705.1">
        <property type="protein sequence ID" value="ENSBIXP00000012654.1"/>
        <property type="gene ID" value="ENSBIXG00000017718.1"/>
    </dbReference>
    <dbReference type="OMA" id="WRAPIYG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="408" mass="46992" checksum="37C72E07D3C0DC0F" modified="2019-09-18" version="1">MLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANICWIIEKARELQKNIYFCFIDYAKAFDCVDHYKLWKILKEMGIPHHLICLLRNLYAGQEATVRIGHGTTDWFQIGKGVHQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEEVGLKLNIQKTKIMASGPITSWEIEGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSILKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAECRRIDAFELWCWRRLLRVPRTARRSNPSILKELSPGISLEGMMLKLKLQYFGHLMRRVDSLEKTVMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVMDREAWRAPIYGVKKSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLJ4</accession>
    <name>A0A4W2CLJ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34" type="primary">ACOX1</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids. Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + O2 = (2E,5Z,8Z,11Z,14Z,17Z)-eicosahexaenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:69643"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:73862"/>
            <dbReference type="ChEBI" id="CHEBI:187901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:69644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + O2 = (2E,6Z,9Z,12Z,15Z,18Z,21Z)-tetracosaheptaenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:39119"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:74086"/>
            <dbReference type="ChEBI" id="CHEBI:76360"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:39120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:38960"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>decanoyl-CoA + O2 = (2E)-decenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40179"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dodecanoyl-CoA + O2 = (2E)-dodecenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40171"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:40172"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>glutaryl-CoA + O2 = (2E)-glutaconyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40315"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57353"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:40316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hexadecanedioyl-CoA + O2 = (2E)-hexadecenedioyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40275"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:77075"/>
            <dbReference type="ChEBI" id="CHEBI:77085"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:40276"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hexadecanoyl-CoA + O2 = (2E)-hexadecenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40167"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:40168"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hexanoyl-CoA + O2 = (2E)-hexenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40311"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40312"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>octadecanoyl-CoA + O2 = (2E)-octadecenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38971"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:38972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>octanoyl-CoA + O2 = (2E)-octenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40175"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:40176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>tetracosanoyl-CoA + O2 = (2E)-tetracosenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40319"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40320"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>tetradecanoyl-CoA + O2 = (2E)-tetradecenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40183"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40184"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLJ4"/>
    <dbReference type="SMR" id="A0A4W2CLJ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002337.1">
        <property type="protein sequence ID" value="ENSBIXP00000007176.1"/>
        <property type="gene ID" value="ENSBIXG00000013167.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000038">
        <property type="term" value="P:very long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01150">
        <property type="entry name" value="AXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000006">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034171">
        <property type="entry name" value="ACO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF250">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 27 29" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 27" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="32">
        <location>
            <begin position="15"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="31">
        <location>
            <begin position="136"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="33">
        <location>
            <begin position="275"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="30">
        <location>
            <begin position="480"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="28">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="660" mass="74379" checksum="606F86A63915DF9D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CM48</accession>
    <name>A0A4W2CM48_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-X-C chemokine receptor type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">High affinity interleukin-8 receptor B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CXCR2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with IL8. Interacts with GNAI2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027423104.1">
        <property type="nucleotide sequence ID" value="XM_027567303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CM48"/>
    <dbReference type="SMR" id="A0A4W2CM48"/>
    <dbReference type="STRING" id="30522.A0A4W2CM48"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025001.1">
        <property type="protein sequence ID" value="ENSBIXP00000014196.1"/>
        <property type="gene ID" value="ENSBIXG00000003330.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034274.1">
        <property type="protein sequence ID" value="ENSBIXP00005020778.1"/>
        <property type="gene ID" value="ENSBIXG00005023802.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907690"/>
    <dbReference type="CTD" id="3579"/>
    <dbReference type="GeneTree" id="ENSGT01050000244848"/>
    <dbReference type="OMA" id="GNQTTKW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016494">
        <property type="term" value="F:C-X-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019959">
        <property type="term" value="F:interleukin-8 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15178">
        <property type="entry name" value="7tmA_CXCR1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000157">
        <property type="entry name" value="C-X-C chemokine receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000057">
        <property type="entry name" value="Chemokine_CXCR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000174">
        <property type="entry name" value="Chemokine_CXCR_1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF689">
        <property type="entry name" value="C-X-C CHEMOKINE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00427">
        <property type="entry name" value="INTRLEUKIN8R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00573">
        <property type="entry name" value="INTRLEUKN8BR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="68"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="363" mass="40928" checksum="DCBF8D1869CB7EB7" modified="2019-09-18" version="1">MAETKFTSNIEGFNWENYSDEDFGNYSYNTDLPSILTDSAPCRPEILDINKHAVVVIYALVFLLSLLGNSLVMLVILYSRIGRSVTDVYLLNLAMADLLFAMTLPIWAASKAKGWVFGTPLCKVVSLLKEVNFYSGILLLACISMDRYLAIVHATRTLTQKRHWVKFICLGIWALSVILALPVFIFRRAIHPPYSSAVCYEDMGANTTKWRMVMRVLPQTFGFLLPLLVMLFCYGFTLRTLFSAQMGQKHRAMRVIFAVVLVFLLCWLPYNLVLIVDTLMRAHVIAETCQRRNDIGRALDATEILGFLHSCLNPLIYVFIGQKFRHGLLKIMAIHGLISKEFLAKDGRPSFVGSSSGNTSTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CP26</accession>
    <name>A0A4W2CP26_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Zinc finger protein 133</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CP26"/>
    <dbReference type="STRING" id="30522.A0A4W2CP26"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046168.1">
        <property type="protein sequence ID" value="ENSBIXP00000013703.1"/>
        <property type="gene ID" value="ENSBIXG00000018589.1"/>
    </dbReference>
    <dbReference type="OMA" id="QKFHMQH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000601">
        <property type="entry name" value="Histone-lysine N-methyltransferase PRDM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000706">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000155">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000189">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001070">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001353">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000566">
        <property type="entry name" value="zinc finger protein 133 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="16">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="323"/>
            <end position="350"/>
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        <location>
            <begin position="351"/>
            <end position="378"/>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="406"/>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="407"/>
            <end position="434"/>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="490"/>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="575"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="71571" checksum="9C4755AA8584E4F5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTH2</accession>
    <name>A0A4W2CTH2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PAT complex subunit CCDC47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coiled-coil domain-containing protein 47</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CCDC47</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein. Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex. Involved in the regulation of calcium ion homeostasis in the ER. Required for proper protein degradation via the ERAD (ER-associated degradation) pathway. Has an essential role in the maintenance of ER organization during embryogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Rough endoplasmic reticulum membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCDC47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027373407.1">
        <property type="nucleotide sequence ID" value="XM_027517606.1"/>
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    <dbReference type="SMR" id="A0A4W2CTH2"/>
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    </dbReference>
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        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610580" description="PAT complex subunit CCDC47" evidence="10">
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    </feature>
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    </feature>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009587.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="483" mass="55742" checksum="E7AC14D738117483" modified="2019-09-18" version="1">MKGFHAFCVILLIFGSVSEAKFDDFEDEEDIVEYDDNDFAEFEDVAEDSVTESPQRVIITEDDEDETTVELEGQDESQEGDFEDADTQEGDTESEPYDDEEFEGYEDKPDTSSSKSKDPITIVDVPAHLQNSWESYYLEILMVTGLLAYIMNYIIGKNKNSRLAQAWFNTHRELLESNFTLVGDDGTNKEATSTGKLNQENEHIYNLWCSGRVCCEGMLIQLRFLKRQDLLNVLARMMRPVSDQVQIKVTMNDEDMDTYVFAVGARKALVRLQKEMQDLSEFCSDKPKSGAKYGLPDSLAILSEMGEVTDGMMDTKMLHFLTHYADKIESIHFSDQFSGPKIMQEEGQPLKLPDTKRTLLFTFNVPGSGNTYPKDMEALLPLMNMVIYSIDKAKKFRLNREGKQKADKNRARVEENFLKLTHVQRQEAAQSRREEKKRAEKERIMNEEDPEKQRRLEEAALRREQKKLEKKQMKMKQIKVKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW22</accession>
    <name>A0A4W2CW22_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CNBD2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Essential for male fertility. Plays an important role in spermatogenesis and regulates sperm motility by controlling the development of the flagellar bending of sperm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027414463.1">
        <property type="nucleotide sequence ID" value="XM_027558662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW22"/>
    <dbReference type="STRING" id="30522.A0A4W2CW22"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030210.1">
        <property type="protein sequence ID" value="ENSBIXP00000017606.1"/>
        <property type="gene ID" value="ENSBIXG00000021601.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008227.1">
        <property type="protein sequence ID" value="ENSBIXP00005030330.1"/>
        <property type="gene ID" value="ENSBIXG00005010424.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903071"/>
    <dbReference type="CTD" id="140894"/>
    <dbReference type="GeneTree" id="ENSGT00390000003964"/>
    <dbReference type="OMA" id="KTTKPCY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000083">
        <property type="entry name" value="Cyclic nucleotide binding domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF43">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="3" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="116"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="583" mass="68441" checksum="0D6D19A5F0AF8BF5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWI1</accession>
    <name>A0A4W2CWI1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab9 effector protein with kelch motifs</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RABEPK</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Rab9 effector required for endosome to trans-Golgi network (TGN) transport.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027412062.1">
        <property type="nucleotide sequence ID" value="XM_027556261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWI1"/>
    <dbReference type="SMR" id="A0A4W2CWI1"/>
    <dbReference type="STRING" id="30522.A0A4W2CWI1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028027.1">
        <property type="protein sequence ID" value="ENSBIXP00000016204.1"/>
        <property type="gene ID" value="ENSBIXG00000000359.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901647"/>
    <dbReference type="CTD" id="10244"/>
    <dbReference type="OMA" id="CTPGSIW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052124">
        <property type="entry name" value="Rab9_kelch_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647:SF1">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="309"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="40362" checksum="7A9169FEC2B99B93" modified="2019-09-18" version="1">MKQLPVLEPGDKPRKETWYTLTLVGDSPCARVGHSCSYLPPVGDAERGKVFIVGGADPNRSFSDVHTIDLGTHQWDLATSEGLLPRYEHTSFIPSCTPHSIWVFGGADQSGNRNCLQVLNPDTRTWTTPEVTGPPPSPRTFHTSSAAIGDQLYVFGGGERGAQPVQDVQLHVFDANTLTWSQPETHGKPPSPRHGHVMVAAGTKLFIHGGLAGDNFYDDLHCIDISDMKWQKLRPTGAAPTGCAAHSAVAVGKHLYVFGGMTPTGALNTMYQYHIEKQHWTLLKFENSPPTGRLDHSMCIIPWPGTCTSEKEDSNSATVNRDAEKGDSTEKGVTQGGDSQEESQADTLLCFVFGGMNTEGEIYDDCIVTAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXC6</accession>
    <name>A0A4W2CXC6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction epsilon-1 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Connexin-23</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the connexin family. Beta-type (group I) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXC6"/>
    <dbReference type="STRING" id="30522.A0A4W2CXC6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028589.1">
        <property type="protein sequence ID" value="ENSBIXP00000016568.1"/>
        <property type="gene ID" value="ENSBIXG00000020735.1"/>
    </dbReference>
    <dbReference type="OMA" id="WLQIYLF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000004">
        <property type="entry name" value="Gap junction epsilon-1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF1">
        <property type="entry name" value="GAP JUNCTION EPSILON-1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="125"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="205" mass="23676" checksum="81BE03AA7A37F520" modified="2019-09-18" version="1">MSLNYIKNFYEGCMKPPTVIGQFHTLFFGSVRMFFLGVLGFAVYGNEALHFSCDPDKREINLFCYNQFRPITPQVFWALQLVIVLVPGAIFHLYAACKSINQECILQKPIYTVIYILSVLLRISLEVVAFWLQIHLFGFQVKPLYLCDAVSLGKKLTIIKCMVPEHFEKTIFLIAMYTFTAITIVLCVAEIFEIIFRRLCFLISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXQ9</accession>
    <name>A0A4W2CXQ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heteroous nuclear ribonucleoprotein L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HNRNPL</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027371694.1">
        <property type="nucleotide sequence ID" value="XM_027515893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXQ9"/>
    <dbReference type="SMR" id="A0A4W2CXQ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020414.1">
        <property type="protein sequence ID" value="ENSBIXP00000011121.1"/>
        <property type="gene ID" value="ENSBIXG00000002549.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000457.1">
        <property type="protein sequence ID" value="ENSBIXP00005032881.1"/>
        <property type="gene ID" value="ENSBIXG00005013499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876544"/>
    <dbReference type="CTD" id="3191"/>
    <dbReference type="GeneTree" id="ENSGT01030000234642"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12780">
        <property type="entry name" value="RRM1_hnRNPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12785">
        <property type="entry name" value="RRM2_hnRNPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12699">
        <property type="entry name" value="RRM3_hnRNPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12704">
        <property type="entry name" value="RRM4_hnRNPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000072">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein L isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000052">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000073">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000104">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006536">
        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034816">
        <property type="entry name" value="hnRNP-L_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035005">
        <property type="entry name" value="hnRNPL_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035008">
        <property type="entry name" value="hnRNPL_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034817">
        <property type="entry name" value="hnRNPL_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="101"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="192"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="381"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="283"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="588" mass="64076" checksum="A4CBEF5682F6EAA9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZF2</accession>
    <name>A0A4W2CZF2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">NAD-capped RNA hydrolase NUDT12</fullName>
            <ecNumber evidence="6">3.6.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">NADH pyrophosphatase NUDT12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Nucleoside diphosphate-linked moiety X motif 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">NUDT12</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to nutrient stress. Also acts on free nicotinamide adenine dinucleotide molecules: hydrolyzes NAD(H) into NMN(H) and AMP, and NADPH into NMNH and 2',5'-ADP. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle. Regulates the levels of circadian clock components PER1, PER2, PER3 and CRY2 in the liver.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>NAD(+) + H2O = beta-nicotinamide D-ribonucleotide + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11800"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:11801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>NADH + H2O = reduced beta-nicotinamide D-ribonucleotide + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90832"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NADPH + H2O = reduced beta-nicotinamide D-ribonucleotide + adenosine 2',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:90832"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:60821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer. Homodimerization is essential for its catalytic activity and protein stability. Interacts (via ANK repeats) with BLMH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027401282.1">
        <property type="nucleotide sequence ID" value="XM_027545481.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401283.1">
        <property type="nucleotide sequence ID" value="XM_027545482.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401285.1">
        <property type="nucleotide sequence ID" value="XM_027545484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZF2"/>
    <dbReference type="STRING" id="30522.A0A4W2CZF2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032120.1">
        <property type="protein sequence ID" value="ENSBIXP00000018839.1"/>
        <property type="gene ID" value="ENSBIXG00000022607.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000150.1">
        <property type="protein sequence ID" value="ENSBIXP00005027721.1"/>
        <property type="gene ID" value="ENSBIXG00005030170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894955"/>
    <dbReference type="CTD" id="83594"/>
    <dbReference type="GeneTree" id="ENSGT00940000157592"/>
    <dbReference type="OMA" id="CNTRTTL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000210">
        <property type="term" value="F:NAD+ diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990174">
        <property type="term" value="F:phosphodiesterase decapping endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110156">
        <property type="term" value="P:mRNA methylguanosine-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:001067">
        <property type="entry name" value="Nudix (Nucleoside diphosphate linked moiety X)-type motif 12 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000023">
        <property type="entry name" value="Peroxisomal NADH pyrophosphatase NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.20:FF:000002">
        <property type="entry name" value="Peroxisomal NADH pyrophosphatase NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015376">
        <property type="entry name" value="Znr_NADH_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001299">
        <property type="entry name" value="PRK00241.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09297">
        <property type="entry name" value="Zn_ribbon_NUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 26" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="ANK" evidence="26">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="27">
        <location>
            <begin position="319"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023869"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031178"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045837"/>
        </source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00049264"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="462" mass="52134" checksum="EE993819F62B908B" modified="2019-09-18" version="1">MSSVKRSLNQEIISQFHYSAAEGDIAKLTAILSHSPSLLNETSENGWSALMYAARNGHPDVVQFLLEKGCDRSIVNKSRQTALDIAKFWGYKHIANLLANAKGGKKPWFLTNEVEECENYFSKTLLDRKSEKRNNSDWLLAKESHPATVYILFSDLNPLVTLGGNKESFQQPEVRLCQLNYTDIKDYLAQPEKITLIFLGVELEMKKEFFNYAGEISKEEEDGLVAWFALGIDTVAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSRYKFCPTCGNATKIEEGGYKRVCLKEDCPSLHGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGVKVGHVQYVSCQPWPMPSSLMIGCLAVAVSTEIKVDKNEIEDARWFTREQVVDVLTKGKQQAFFVPPSRAIAHQLIKHWIGMNPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D075</accession>
    <name>A0A4W2D075_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Growth/differentiation factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Bone morphogenetic protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GDF2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer; disulfide-linked. Detected in extracellular fluid as mature homodimer, and in complex with its propeptide. Interacts with ACVRL1, BMPR2 and ACVR2B with high affinity (in vitro). Identified in a complex with ACVRL1 and ACVR2B. Has ten times lower affinity for ACVR2A (in vitro). Interacts with ENG, forming a heterotetramer with a 2:2 stoichiometry. Can form a heteromeric complex with ENG and ACVRL1. Interacts with type I receptor ACVR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386645.1">
        <property type="nucleotide sequence ID" value="XM_027530844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D075"/>
    <dbReference type="SMR" id="A0A4W2D075"/>
    <dbReference type="STRING" id="30522.A0A4W2D075"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032594.1">
        <property type="protein sequence ID" value="ENSBIXP00000019129.1"/>
        <property type="gene ID" value="ENSBIXG00000022813.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055998.1">
        <property type="protein sequence ID" value="ENSBIXP00005046275.1"/>
        <property type="gene ID" value="ENSBIXG00005031173.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885476"/>
    <dbReference type="CTD" id="2658"/>
    <dbReference type="GeneTree" id="ENSGT00940000159802"/>
    <dbReference type="OMA" id="GTFDLRM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001569">
        <property type="term" value="P:branching involved in blood vessel morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010596">
        <property type="term" value="P:negative regulation of endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001937">
        <property type="term" value="P:negative regulation of endothelial cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903348">
        <property type="term" value="P:positive regulation of bicellular tight junction assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061036">
        <property type="term" value="P:positive regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045603">
        <property type="term" value="P:positive regulation of endothelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032757">
        <property type="term" value="P:positive regulation of interleukin-8 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045747">
        <property type="term" value="P:positive regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060391">
        <property type="term" value="P:positive regulation of SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19400">
        <property type="entry name" value="TGF_beta_BMP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000014">
        <property type="entry name" value="growth/differentiation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF157">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0339">Growth factor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610638" description="Growth/differentiation factor 2" evidence="16">
        <location>
            <begin position="23"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="17">
        <location>
            <begin position="318"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="431" mass="48231" checksum="14A9BF874B2D418A" modified="2019-09-18" version="1">MGRGALWVALPVLCLLACSALGKPLENRGRPSTGADAHGLLGGPGGEQEGVTFDLRMFLENMKVDFLRNLNLSGVPSQDRTRAEPPQYMIDLYNRYTTDKTSTPASNIVRSFSVEDAVSIATTEDFPFQKHILLFNISIPRHEQITRAELRLYLSCQSHMDSPHELKGNMVIYDVLDGAEVWDAPAGTKTFLVSQDIRDEGWETFEVSSAVKRWIRADSTKSKNKLEVTVESHRKGCDKLDISVPPGSKNLPFFVVFSNDRSNGTKETRLELREMIGHEQESVLRKLSKNTVVEAGENKDEEEEDVRSHTPTESSLVRRKRSTGANNHCQKTSLRVNFEDIGWDSWIIAPKEYDAFECKGGCFFPLADDVTPTKHAIVKTLVHLKFPMKVGKACCVPTKLSPISILYKDDMGVPTLKYHYEGMSVAECGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0L7</accession>
    <name>A0A4W2D0L7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein SERAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serine active site-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SERAC1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Facilitates the transport of serine from the cytosol to the mitochondria by interacting with and stabilizing Sideroflexin-1 (SFXN1), a mitochondrial serine transporter, playing a fundamental role in the one-carbon cycle responsible for the synthesis of nucleotides needed for mitochondrial DNA replication. Plays an important role in the phosphatidylglycerol (PG) remodeling that is essential for both mitochondrial function and intracellular cholesterol trafficking. Specifically involved in the exchange of the sn-1 acyl chain from PG 16:0/18:1(9Z) (also known as 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)) to PG 18:0/18:1(9Z) (also known as 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)), a step needed in the bis(monoacylglycerol)phosphate biosynthetic pathway. May have acyltransferase activity although the mechanism for PG remodeling has not been determined.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the SERAC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027407742.1">
        <property type="nucleotide sequence ID" value="XM_027551941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D0L7"/>
    <dbReference type="STRING" id="30522.A0A4W2D0L7"/>
    <dbReference type="ESTHER" id="bovin-srac1">
        <property type="family name" value="SERAC1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000030428.1">
        <property type="protein sequence ID" value="ENSBIXP00000017758.1"/>
        <property type="gene ID" value="ENSBIXG00000021717.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022136.1">
        <property type="protein sequence ID" value="ENSBIXP00005035646.1"/>
        <property type="gene ID" value="ENSBIXG00005017174.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898619"/>
    <dbReference type="CTD" id="84947"/>
    <dbReference type="GeneTree" id="ENSGT00390000003560"/>
    <dbReference type="OMA" id="RRTEYIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036148">
        <property type="term" value="P:phosphatidylglycerol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000088">
        <property type="entry name" value="SERAC1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="654" mass="74196" checksum="E75298FC8A593D98" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D134</accession>
    <name>A0A4W2D134_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ciliary microtubule inner protein 2C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CIMIP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D134"/>
    <dbReference type="SMR" id="A0A4W2D134"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030670.1">
        <property type="protein sequence ID" value="ENSBIXP00000017919.1"/>
        <property type="gene ID" value="ENSBIXG00000021834.1"/>
    </dbReference>
    <dbReference type="OMA" id="RQKRDCY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052329">
        <property type="entry name" value="CIMIP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018902">
        <property type="entry name" value="CMI2A-C-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34924:SF1">
        <property type="entry name" value="PROTEIN FAM166C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34924">
        <property type="entry name" value="UPF0573 PROTEIN C2ORF70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10629">
        <property type="entry name" value="CMI2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="201" mass="23379" checksum="89F0D38DF28643D7" modified="2019-09-18" version="1">MASRSAGTLLTEFNAAYVPPGLMPGYKGHVPGVAFSFGSPYGTTTLKYFQDQRNAALGRSSTAFSRGGHFPTIFSLNPTQVLRNRALTRDRWLHTPSYTRFNLDSSRWAELLHFYQMAQRHREHYQDKTGLVHRVPYFVLPVKEWDRYPIPTDLPPLSPKEKWHLLRVAPENLRTYQTFPSGKRVSPQERQRRDCYFEFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D182</accession>
    <name>A0A4W2D182_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich PDZ-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cysteine-rich interactor of PDZ three</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CRIPT</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410983.1">
        <property type="nucleotide sequence ID" value="XM_027555182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D182"/>
    <dbReference type="SMR" id="A0A4W2D182"/>
    <dbReference type="STRING" id="30522.A0A4W2D182"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022292.1">
        <property type="protein sequence ID" value="ENSBIXP00000012397.1"/>
        <property type="gene ID" value="ENSBIXG00000017494.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049200.1">
        <property type="protein sequence ID" value="ENSBIXP00005018296.1"/>
        <property type="gene ID" value="ENSBIXG00005021712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901172"/>
    <dbReference type="CTD" id="9419"/>
    <dbReference type="GeneTree" id="ENSGT00950000183100"/>
    <dbReference type="OMA" id="MPCDKCE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097110">
        <property type="term" value="F:scaffold protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019367">
        <property type="entry name" value="PDZ-binding_CRIPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805:SF1">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10235">
        <property type="entry name" value="Cript"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="101" mass="11216" checksum="161B0F4CA87DFBAD" modified="2019-09-18" version="1">MVCEKCEKKLGTVITPDTWKDGARNTTESGGRKLNENKALTSKKARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1C2</accession>
    <name>A0A4W2D1C2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Alpha-amylase</fullName>
            <ecNumber evidence="5 15">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC113889545</name>
        <name evidence="20" type="synonym">LOC113889547</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="5 15"/>
    <dbReference type="RefSeq" id="XP_027392161.1">
        <property type="nucleotide sequence ID" value="XM_027536360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1C2"/>
    <dbReference type="SMR" id="A0A4W2D1C2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033308.1">
        <property type="protein sequence ID" value="ENSBIXP00000019626.1"/>
        <property type="gene ID" value="ENSBIXG00000023223.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005428.1">
        <property type="protein sequence ID" value="ENSBIXP00005031212.1"/>
        <property type="gene ID" value="ENSBIXG00005011773.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889545"/>
    <dbReference type="GeneTree" id="ENSGT00940000154802"/>
    <dbReference type="OMA" id="FRYAYDL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11317">
        <property type="entry name" value="AmyAc_bac_euk_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000020">
        <property type="entry name" value="Pancreatic alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000056">
        <property type="entry name" value="Pancreatic alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031319">
        <property type="entry name" value="A-amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00632">
        <property type="entry name" value="Aamy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610651" description="Alpha-amylase" evidence="16">
        <location>
            <begin position="16"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="18">
        <location>
            <begin position="26"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase C-terminal" evidence="17">
        <location>
            <begin position="422"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="511" mass="57041" checksum="EFB1CC1577F144D5" modified="2019-09-18" version="1">MKFFLLLSAIGFCWAQYDPHVKSGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPSENAVITDPSRPWWERYQPVSYKLCTRSGNENEFKDMVTRCNNVGVRIYVDAVINHMTGSGVSAGTGSTCGSYFNPGSEDFPAVPYSGWDFNDGKCKTGSGDIESYNDAYQVRDCRLVSLLDLALEKDYVRSTIANYLNHLIDIGVAGFRIDASKHMWPGDIKAVLDKLHNLNTKWFPSGSKPFIYQEVIDLGGEAITSSEYFGNGRVTEFKYGAKLGTVIRKWSGEKMAYLKNWGEGWGFMPSNRALVFVDNHDNQRGHGAGGASILTFWDARLYKMGVGFMLAHPYGFTRVMSSYRWTRHFVNGKDVNDWMGPPNKNGVIKEVTINPDTTCGNDWVCEHRWRQIRNMVMFRNVVDGQPFTNWWDNGSNQVAFGRGNKGFIVFNNDDSTLSATLQTGLPAGTYCDVISGDKSGNSCTGIQISVSSDGKAHFSISNSAEDPFIAIHADSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4Q0</accession>
    <name>A0A4W2D4Q0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Subunit of the 40S ribosomal complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Subunit of the 40S ribosomal complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4Q0"/>
    <dbReference type="STRING" id="30522.A0A4W2D4Q0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032697.1">
        <property type="protein sequence ID" value="ENSBIXP00000019199.1"/>
        <property type="gene ID" value="ENSBIXG00000004474.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="8">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="136" mass="15305" checksum="563BAA70061F44B1" modified="2019-09-18" version="1">SSVPRPCCRHPHETLRFEVLKAALIHDGIPACGSHEAAKALDEHQAHPRVLVSNCDEPTYVKLVEALCDEHQISLIEVDNNKKLREWVGLCKTDREGKPHQVVWWLRTMAKNLGPRTSSRSTSNARNDEIKNGVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D526</accession>
    <name>A0A4W2D526_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">YPEL</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D526"/>
    <dbReference type="STRING" id="30522.A0A4W2D526"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046224.1">
        <property type="protein sequence ID" value="ENSBIXP00000013812.1"/>
        <property type="gene ID" value="ENSBIXG00000018675.1"/>
    </dbReference>
    <dbReference type="OMA" id="HLAFKGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="19"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="119" mass="13560" checksum="629FBDB42B6D4801" modified="2019-09-18" version="1">MVKMTKSKTFQAYLPNCHRTYSCVHCRAHLANHDELISKSFQGSQGRAYLFNSVVNVGCGPAEERVLLTGLHAVADIYCENCKTTLGWKYEHAFESSQKYKEGKFIIELAHMIKDNGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5F7</accession>
    <name>A0A4W2D5F7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5F7"/>
    <dbReference type="STRING" id="30522.A0A4W2D5F7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048953.1">
        <property type="protein sequence ID" value="ENSBIXP00000019452.1"/>
        <property type="gene ID" value="ENSBIXG00000023060.1"/>
    </dbReference>
    <dbReference type="OMA" id="FIIPSQH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051896">
        <property type="entry name" value="TCR_alpha_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19339:SF2">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19339">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021233151" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="22"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="182" mass="19675" checksum="EDEF3D1F8FCDFD6B" modified="2019-09-18" version="1">MKRLAGTVLGLLFAQVCCVQGVDVEQSPPALSLQKAASYTLRCNFSTSSQSVNWNLQNPGGTKQDGRLNATTVPKEGRSSLHISSSQTTDSGTYFCAVQHSAPQAPAASTQTLRGAQPLPQPQSHREATTGHLQCSSVLIFISTVLTTDTFWPYRLWTLVFIIPSQHVSPSALETSNWELAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D975</accession>
    <name>A0A4W2D975_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
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        <source>
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    </evidence>
    <sequence length="343" mass="39445" checksum="929265AE0DD377FB" modified="2019-09-18" version="1">MLKILQARLQQYMNHELPDVHAGFRKGRGTRDQIANICWIIKKAREFQKNIYFCFIDYAKAFDSVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHRITDWFQIGKGVCQGCILSPCLFNLYAEYIKRNTGLEEAQAGIKIAGRNINNLRYADDTTLKAESEEELKGLLMKVKEESKKVGLRLNIQKTKIMASGPITSWQIDGETVATVSDFIFLGSKITADGDCSHEIKRHLLLGRKVMTNLESIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAECQRTDAFELWCWRRLLRVPWIARRSNQSILKEISPECSLEGLMLKLKLQYLGHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAG6</accession>
    <name>A0A4W2DAG6_BOBOX</name>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBB7</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDH9</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="12" type="primary">LOC113896576</name>
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    </gene>
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        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006591">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial ornithine transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF20">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="104"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="207"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="301" mass="32719" checksum="9812E3FC14AE71EE" modified="2019-09-18" version="1">MKSSPAIQAAIDLTAGALGGTACVLTGQPFDTMKVKMQTFPGLYKGLTDCALKTYSQVGLRGFYKGTGPALMAYVAENSVLFMCYGFCQHFVRKVAGLDEQAKLNDLQTAAAGSIASAFAALALCPTELVKCRLQTMHELEMSGRIAKSHDTVWSVVKSILRKDGPWGFYHGLTSTLFQVVPGYFFFFGGYELSRSFFASGGSKDELGPVPLMLSGGIAGICLWFVIYPVDCIKSRIQVLSMFGKQTGFIRTLLSVVRTEGIAALYSGLKATLIRAFPANAALFLAYEYSRKMMMSQFEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DE72</accession>
    <name>A0A4W2DE72_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="12">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UBR3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May play a role in Shh signaling by mediating the ubiquitination of Kif7. May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with UBE2A and UBE2B.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="12"/>
    <dbReference type="STRING" id="30522.A0A4W2DE72"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037687.1">
        <property type="protein sequence ID" value="ENSBIXP00000022495.1"/>
        <property type="gene ID" value="ENSBIXG00000025320.1"/>
    </dbReference>
    <dbReference type="OMA" id="LTRAWCT"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16483">
        <property type="entry name" value="RING-H2_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF39">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="14">
        <location>
            <begin position="118"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="11">
        <location>
            <begin position="118"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1888" mass="212581" checksum="E3D8B9086FA5E023" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFG6</accession>
    <name>A0A4W2DFG6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Lysine-specific demethylase 4A</fullName>
            <ecNumber evidence="4">1.14.11.66</ecNumber>
            <ecNumber evidence="22">1.14.11.69</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">JmjC domain-containing histone demethylation protein 3A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Jumonji domain-containing protein 2A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">[histone H3]-trimethyl-L-lysine(36) demethylase 4A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">[histone H3]-trimethyl-L-lysine(9) demethylase 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">KDM4A</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60236"/>
            <dbReference type="Rhea" id="RHEA-COMP:9786"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.69"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="4"/>
    <dbReference type="EC" id="1.14.11.69" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFG6"/>
    <dbReference type="SMR" id="A0A4W2DFG6"/>
    <dbReference type="STRING" id="30522.A0A4W2DFG6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050796.1">
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    <sequence length="1066" mass="120827" checksum="3B975D4C2C1849F3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFY6</accession>
    <name>A0A4W2DFY6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="11">Perilipin</fullName>
        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="9">Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.</text>
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        <text evidence="10">Interacts with IRGC.</text>
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        <subcellularLocation>
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        <subcellularLocation>
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        <property type="term" value="P:positive regulation of triglyceride storage"/>
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    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="447" mass="49072" checksum="A832EBFC17475068" modified="2019-09-18" version="1">MASVAVEPQLSVVTRVANLPLVSSTYDLVSSAYISRKDQYPYLKSLCEMAEKGTKTITSVAVTSALPIIQKLEPQIAVANTYACKGLDRIEEKLPILNQPTNQVVANAKGAMTGAKDAVTTTVTGAKDSVASTITGVVDRTKGAVTGSVEKTKSVVSGSINTVLRSRVMQLMSSGVENALTKSELLVDQYLPLTKDELEKEAKKVEGFDMVQKPSYYVRLGSLSTKLRSRAYQQALCRVEEAKRKGQETISQLHSAFNLSELARKNVHNANQKIQDAQDKLYLSWLEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTMCHTLLSNIQGLPQNIQDRANHLGVMAGDIYSVFRNAASFKEVSDGLLASSKGQLQKMKESLDDVMDYLVNNTPLNWLALDFSITDFTSETDEIPDIIALEEEDGPNHSHANGPEPSQGKMLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DH53</accession>
    <name>A0A4W2DH53_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="19">PARP</shortName>
            <ecNumber evidence="19">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PARP2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LRIG3. Homo- and heterodimer with PARP1. Interacts (via the PARP catalytic domain) with HPF1. Interacts with core nucleosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DH53"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031008.1">
        <property type="protein sequence ID" value="ENSBIXP00000018151.1"/>
        <property type="gene ID" value="ENSBIXG00000004263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22252">
        <property type="entry name" value="PARP2_NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08003">
        <property type="entry name" value="WGR_PARP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000003">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.140.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.140.10">
        <property type="entry name" value="WGR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WGR" evidence="23">
        <location>
            <begin position="83"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="22">
        <location>
            <begin position="210"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="21">
        <location>
            <begin position="335"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="562" mass="63831" checksum="D545F4B52B2124C5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKN7</accession>
    <name>A0A4W2DKN7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fc receptor like 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FCRL6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027384762.1">
        <property type="nucleotide sequence ID" value="XM_027528961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKN7"/>
    <dbReference type="SMR" id="A0A4W2DKN7"/>
    <dbReference type="STRING" id="30522.A0A4W2DKN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040946.1">
        <property type="protein sequence ID" value="ENSBIXP00000024667.1"/>
        <property type="gene ID" value="ENSBIXG00000026800.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022662.1">
        <property type="protein sequence ID" value="ENSBIXP00005035859.1"/>
        <property type="gene ID" value="ENSBIXG00005017159.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884373"/>
    <dbReference type="CTD" id="343413"/>
    <dbReference type="GeneTree" id="ENSGT01050000244808"/>
    <dbReference type="OMA" id="GTQRSEM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042289">
        <property type="term" value="F:MHC class II protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000357">
        <property type="entry name" value="Fc receptor like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000651">
        <property type="entry name" value="Fc receptor like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF62">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610835" evidence="11">
        <location>
            <begin position="16"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="111"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="207"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="423" mass="47192" checksum="99909FF82F66C5FA" modified="2019-09-18" version="1">MLLWTVALLFVPCVGKIVWLSPQAQPQLVFEGDILILRCRGRKNAALSHVKFYRDGKFLHFSMENQPLLLGTATANSSGWYNCTGRVKYPRNMDSWDSGTAMVQVQELFLPPVLTALPSHELCEGSPVTLRCQTKLHSQKSASRLLFSFHKEGRTLKNRSSRPELRIPAAKEGDAGLYWCKASSEGGQIQKRSPQLELRVWAPVSRPLLTLRPTSLAVGDEVELLCEVQRGSPPILYSFHLNGDILRNHVAPHGGPASCLFRVTSEQDAGNYSCEAGNRVSRETSEPETLSVDDPQVLSAPTSSNWLVPWLPASLLAMMVIAAALLGYCRPWRKNGPLPPRNLPSAPSEEQHPLYVNVYHQNENNEGVIYSEIHTITIPREHEARPAQPAQQEKDISVIYAEVRHPQLSKNPDKGLNRRSTIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLG3</accession>
    <name>A0A4W2DLG3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein c-Fos</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cellular oncogene fos</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FOS</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Fos subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408268.1">
        <property type="nucleotide sequence ID" value="XM_027552467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLG3"/>
    <dbReference type="SMR" id="A0A4W2DLG3"/>
    <dbReference type="STRING" id="30522.A0A4W2DLG3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049080.1">
        <property type="protein sequence ID" value="ENSBIXP00000019721.1"/>
        <property type="gene ID" value="ENSBIXG00000023266.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036426.1">
        <property type="protein sequence ID" value="ENSBIXP00005022187.1"/>
        <property type="gene ID" value="ENSBIXG00005025139.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899131"/>
    <dbReference type="CTD" id="2353"/>
    <dbReference type="GeneTree" id="ENSGT00940000159276"/>
    <dbReference type="OMA" id="NRTHPYG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035976">
        <property type="term" value="C:transcription factor AP-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070412">
        <property type="term" value="F:R-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034614">
        <property type="term" value="P:cellular response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902895">
        <property type="term" value="P:positive regulation of miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045672">
        <property type="term" value="P:positive regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035994">
        <property type="term" value="P:response to muscle stretch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035914">
        <property type="term" value="P:skeletal muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14721">
        <property type="entry name" value="bZIP_Fos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000006">
        <property type="entry name" value="fos-related antigen 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF4">
        <property type="entry name" value="PROTEIN C-FOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="380" mass="40737" checksum="44AE951257F292B0" modified="2019-09-18" version="1">MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDYCTDLAVSSANFIPTVTAISTSPDLQWLVQPTLVSSVAPSQTRAPHPYGVPTPSAGAYSRAGVMKTMTGGRAQSIGRRGKVEQLSPEEEEKRRIRRERNKMAAAKCRNRRRELTDTLQAETDQLEDEKSALQTEIANLLKEKEKLEFILAAHRPACKIPDDLGFPEEMSVASLDLSGGLPEAATPESEEAFTLPLLNDPEPKPSVEPVKSVGSMELKAEPFDDYMFPASSRPSGSETARSVPDMDLSGSFYAADWEPLHGGSLGMGPMATELEPLCTPVVTCTPSCTTYTSSFVFTYPEADSFPSCAAAHRKGSSSNEPSSDSLSSPTLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMC1</accession>
    <name>A0A4W2DMC1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phenylalanine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="4">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">FARS2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMC1"/>
    <dbReference type="SMR" id="A0A4W2DMC1"/>
    <dbReference type="STRING" id="30522.A0A4W2DMC1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012938.1">
        <property type="protein sequence ID" value="ENSBIXP00000027583.1"/>
        <property type="gene ID" value="ENSBIXG00000008466.1"/>
    </dbReference>
    <dbReference type="OMA" id="PISHYPQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000041">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="18">
        <location>
            <begin position="170"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="19">
        <location>
            <begin position="353"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="446" mass="51403" checksum="A654B2706799EAE7" modified="2019-09-18" version="1">MVCWALRRVGLARAHLARKASGVCRGHEHQPLGPRPAAPGAPGSAVELLGKSYPQDDYSNLTRKVLSKVGRNLHNQPQHPLWLLKERVKQHFYAQYPGRAGTPLFSVYDDLSPVVTTWQNFDSLLIPADHPSRKKGDNYFLNATHMLRAHTSAHQWDLLRAGLDAFLVVGDVYRRDQIDAQHYPVFHQLEAVRLFSRHQLFAGIKDGENLQLFEQSSRSAHKQETHTLEATKLVEFDLKQTLTRLMTHIFGDGLDVRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWSQDERFLKQFRVPDINQKVTFQPLSKYPAVINDISFWLPRENYTENDFYDLVRTIGGDLVEKVDLIDKFEHPKTHRTSHCYRITYRHMERTLSQREVGGIHQAVQEAAVRQLGVEGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMQ2</accession>
    <name>A0A4W2DMQ2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Lysophospholipase D GDPD3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Glycerophosphodiester phosphodiesterase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Glycerophosphodiester phosphodiesterase domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">GDPD3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. Shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylcholine (lyso-PC) and N-acylethanolamine lysophospholipids. Does not display glycerophosphodiester phosphodiesterase activity, since it cannot hydrolyze either glycerophosphoinositol or glycerophosphocholine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = 1-O-(1Z-octadecenyl)-sn-glycero-3-phosphate + N-hexadecanoylethanolamine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:137009"/>
            <dbReference type="ChEBI" id="CHEBI:137017"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:53185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41143"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(9Z-octadecenoyl) ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85222"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:56461"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45544"/>
            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85223"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:45545"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoylethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53168"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:53169"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027382391.1">
        <property type="nucleotide sequence ID" value="XM_027526590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMQ2"/>
    <dbReference type="SMR" id="A0A4W2DMQ2"/>
    <dbReference type="STRING" id="30522.A0A4W2DMQ2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012530.1">
        <property type="protein sequence ID" value="ENSBIXP00000027715.1"/>
        <property type="gene ID" value="ENSBIXG00000008633.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050436.1">
        <property type="protein sequence ID" value="ENSBIXP00005020640.1"/>
        <property type="gene ID" value="ENSBIXG00005004061.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883176"/>
    <dbReference type="CTD" id="79153"/>
    <dbReference type="GeneTree" id="ENSGT00940000160759"/>
    <dbReference type="OMA" id="KWAIMRK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034638">
        <property type="term" value="P:phosphatidylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08612">
        <property type="entry name" value="GDPD_GDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000033">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF3">
        <property type="entry name" value="LYSOPHOSPHOLIPASE D GDPD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="23">
        <location>
            <begin position="39"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="318" mass="36590" checksum="E9DE313E5C9C22D6" modified="2019-09-18" version="1">MSPLLYYALPALGSYVMLSFFFLRRPRLLHTPWVPAFRPRLGAHRGGSGERLENTMEAMENSMAQRADFLELDCQLTRDGVVVVSHDKNLSRQTGVNRDVGSLNFEDLPLLKEELEVYFSPGHFARGADRHMMRLEDLFRRFPRTPMSVEVKERNEELIHKIACLVRHYDRNEITIWASEKSSIMKKCKAANPEMPTSFTLSRGFWVLLFYYLGLLPFISIPEKFFMCFLPTIINRTYFPFSHPGLNQLAAVVAKWLIMRKSLIQHLQQRGVQVVFWCLNEESDFEAAFNLGATGVITDYPTALRCYLDSRGPPALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DPK5</accession>
    <name>A0A4W2DPK5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DPK5"/>
    <dbReference type="STRING" id="30522.A0A4W2DPK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010392.1">
        <property type="protein sequence ID" value="ENSBIXP00000028409.1"/>
        <property type="gene ID" value="ENSBIXG00000009565.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001424">
        <property type="entry name" value="Elongation factor 1-alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="41"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="458" mass="49737" checksum="6991F7D991D23B82" modified="2019-09-18" version="1">WVVIRITRNGFLYVISILKIRKLAFSQWVYRQDTGVMKTTSTMTGHLIYKCGGIDKRTIEKFEKEAAKMGKGSFKYAWVLDKLKAERECGITIDISLWKFETSKYYVTITDAPGHRDFIKNMISGTSQADCAVLIVAAGVGEFEAVIGKNGQTREHALLAYTLGVKQLIVGINKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVASVPISGWNGDNMLEPSDGNASGTPLLEALDCILPPTHPTDRPLHLPLEDVYKIGGIGIVPVSHVETGVLKPGMVVTFAPVSVTTEVNGALPGDNVSFNVKNVSVKDVCRGNVAGDSKTDPPMEAAGFTVQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRCSGKKLEDGPKFLKSGDAAIIDMVSAKPMCVESFSDYPPLGRFAVHDMRQTVAVGVIEEVDKKAAGAGKVTKSAQKAQKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRA0</accession>
    <name>A0A4W2DRA0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRA0"/>
    <dbReference type="STRING" id="30522.A0A4W2DRA0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008382.1">
        <property type="protein sequence ID" value="ENSBIXP00000029049.1"/>
        <property type="gene ID" value="ENSBIXG00000010532.1"/>
    </dbReference>
    <dbReference type="OMA" id="PGHVKDS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031529">
        <property type="entry name" value="IHO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35662">
        <property type="entry name" value="INTERACTOR OF HORMAD1 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35662:SF1">
        <property type="entry name" value="INTERACTOR OF HORMAD1 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15771">
        <property type="entry name" value="IHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="592" mass="65677" checksum="7EF698B8E6871618" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTX2</accession>
    <name>A0A4W2DTX2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase activating protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARHGAP8</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTX2"/>
    <dbReference type="SMR" id="A0A4W2DTX2"/>
    <dbReference type="STRING" id="30522.A0A4W2DTX2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005943.1">
        <property type="protein sequence ID" value="ENSBIXP00000029821.1"/>
        <property type="gene ID" value="ENSBIXG00000011553.1"/>
    </dbReference>
    <dbReference type="OMA" id="WLQNAYK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001136">
        <property type="term" value="P:negative regulation of endocytic recycling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04404">
        <property type="entry name" value="RhoGAP-p50rhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000024">
        <property type="entry name" value="Rho GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000007">
        <property type="entry name" value="rho GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049592">
        <property type="entry name" value="ARHGAP1_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF4">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="3">
        <location>
            <begin position="51"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="233"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="426"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="479" mass="54269" checksum="AAEE2167CB7F71F0" modified="2019-09-18" version="1">MSGLDLEELAEIELQRDEEEAAALGAARRPSAAPSVAPPAGPDPELSTNHPFYDVARHGILQVAGEDRFGRRVITFCCCRMPPSHELNHRRLLEYLKYTLDQYVESDYTIVYFHYGLSSQNKPSLRWLQSAYKEFDRRYKKNLKALYVVHPTNFIKILWTILKPLVSHKFGKKVTYFNYLSELREHLKYDQLSIPQEVLRYDEELRNLHAGRLPPPTKTPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRYTVTYLREKGLHTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHLPAVILKTFLRELPQPLLTFEAYEQILEITSVESSLRVTCCRQILQNLPEHNYAVLSYLMGFLHEVSQESISNKMNSSNLACVFGLNLIWPSQGASSLSALVPLNLFTELLIEYYEKVFSGRGERGAEASEQGAPGARGSVPNEGAAAGDRPASGDRPASGLARPPLPPRPLTTAWRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUB0</accession>
    <name>A0A4W2DUB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Bifunctional peptidase and arginyl-hydroxylase JMJD5</fullName>
            <ecNumber evidence="16">1.14.11.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">JmjC domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Jumonji C domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">L-arginine (3R)-hydroxylase KDM8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">KDM8</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-arginyl-[protein] + 2-oxoglutarate + O2 = (3R)-3-hydroxy-L-arginyl-[protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:56744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:14712"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:78294"/>
            <dbReference type="EC" id="1.14.11.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Can form homodimers (via JmjC domain). Found in a complex with RCCD1. Interacts (via N-terminus) with RCCD1 (via N-terminus); this interaction stimulates H3K36me3 and H3K36me2 demethylation. Interacts (via JmjC domain) with H3C1. Interacts with FBXL3 and PSMD2. Interacts with CRY1 in a FBXL3-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.73" evidence="16"/>
    <dbReference type="RefSeq" id="XP_027382322.1">
        <property type="nucleotide sequence ID" value="XM_027526521.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027382323.1">
        <property type="nucleotide sequence ID" value="XM_027526522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUB0"/>
    <dbReference type="SMR" id="A0A4W2DUB0"/>
    <dbReference type="STRING" id="30522.A0A4W2DUB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011432.1">
        <property type="protein sequence ID" value="ENSBIXP00000028067.1"/>
        <property type="gene ID" value="ENSBIXG00000000236.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029531.1">
        <property type="protein sequence ID" value="ENSBIXP00005017593.1"/>
        <property type="gene ID" value="ENSBIXG00005021078.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883149"/>
    <dbReference type="CTD" id="79831"/>
    <dbReference type="GeneTree" id="ENSGT00940000158074"/>
    <dbReference type="OMA" id="KASYQEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106157">
        <property type="term" value="F:peptidyl-arginine 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048144">
        <property type="term" value="P:fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901796">
        <property type="term" value="P:regulation of signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000019">
        <property type="entry name" value="Bifunctional peptidase and arginyl-hydroxylase JMJD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056520">
        <property type="entry name" value="ARM_KDM8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF106">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND ARGINYL-HYDROXYLASE JMJD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24472">
        <property type="entry name" value="ARM_KDM8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="21">
        <location>
            <begin position="261"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="406" mass="45907" checksum="D6A5A5078715E196" modified="2019-09-18" version="1">MAGPSTLWETLQALLPHTKEELKLELGEKVEGSVLMLLQEAAELFLGGQRRECLQTCEVLLDYSWEKLNTGPWQHVDKDWRRVYAFGCLLKAVCLCEPPGDAASVAAALKACDMGLLMGAAILGDILLKVAAVLQKYLLSGKRPAPGPSQEPPGTKKARNDHVPIPDVTTERTVPRLHCPSLQYFKKHFLVPGRPVILEGVANHWPCMKKWSLEYIQEVAGCRTVPVEVGSRYTDEEWSQTLMTVNEFISKYIREEPKDIGYLAQHQLFDQIPELKQDISIPDYCCLGDGEEEEITINAWFGPQGTVSPLHQDPQQNFLAQVMGRKYIRLYSPQESEALYPHDTHLLHNTSQVDVENPDLEKFPRFAEAPFLSCVLSPGEVLFIPVKHWHYVRALDLSFSVSFWWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DW96</accession>
    <name>A0A4W2DW96_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">THAP domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">THAP1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with PAWR. Component of a THAP1/THAP3-HCFC1-OGT complex that contains, either THAP1 or THAP3, HCFC1 and OGT. Interacts with OGT. Interacts (via the HBM) with HCFC1 (via the Kelch-repeat domain); the interaction recruits HCFC1 to the RRM1 promoter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
            <location evidence="1 14">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the THAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027385731.1">
        <property type="nucleotide sequence ID" value="XM_027529930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DW96"/>
    <dbReference type="SMR" id="A0A4W2DW96"/>
    <dbReference type="STRING" id="30522.A0A4W2DW96"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009266.1">
        <property type="protein sequence ID" value="ENSBIXP00000028775.1"/>
        <property type="gene ID" value="ENSBIXG00000010132.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041965.1">
        <property type="protein sequence ID" value="ENSBIXP00005044553.1"/>
        <property type="gene ID" value="ENSBIXG00005028395.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884870"/>
    <dbReference type="CTD" id="55145"/>
    <dbReference type="GeneTree" id="ENSGT00940000159383"/>
    <dbReference type="OMA" id="TKDCFKR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="16">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="142"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="213" mass="24876" checksum="82D066DEE8AD9C2E" modified="2019-09-18" version="1">MVQSCSAYGCKNRYDKDKPVSFHKFPLTRPSLCKKWEAAVRRKNFKPTKYSSICSEHFTPDCFKRECNNKLLKEDAVPTIFLCTEPHDKKEDLLEPQEQPPPPPLTPPISQVDAAIGLLMPPLQTPDNLSVFCDHNYTVEDTMHQRKRIHQLEQQVEKLRKKLKTAQQRCRRQERQLEKLKEVVHFQKEKDGASERGYVILPNDYFEIVEVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWS9</accession>
    <name>A0A4W2DWS9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113881708</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWS9"/>
    <dbReference type="STRING" id="30522.A0A4W2DWS9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001954.1">
        <property type="protein sequence ID" value="ENSBIXP00000031161.1"/>
        <property type="gene ID" value="ENSBIXG00000013344.1"/>
    </dbReference>
    <dbReference type="OMA" id="MTNQSCQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15434">
        <property type="entry name" value="7tmA_OR2W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="306" mass="34084" checksum="7C76DC4B14BDFBAE" modified="2019-09-18" version="1">MTNQSCQEGFILLGFSDRPKLEQILFVFVLIFYLVTLVGNAVIILVSHLDPSLHMPMYFFLTNLSFLDLCFTTSSIPQLLFNLGSPDKTISYVGCAIQLFMFLGLGGTECVLLAVMAYDRFTAICKPLHYSVLMHPQLCWKLVSVAWGVGLLNSLVMSPVTMKLPRCGRCRVKHFLCEMPALIKVACVDTVAVESAVFILSVIIVLVPLSFILISYSCIALAVLRMKSAAGRRKAFNTCGSHLTVVSLFYGNIIYMYMQPGNNSSQDQGKFLTLFYNLVTPMLNPVIYTLRNKDVKGALRRLVSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZW2</accession>
    <name>A0A4W2DZW2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="19">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATP2A3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:18106"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZW2"/>
    <dbReference type="SMR" id="A0A4W2DZW2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017353.1">
        <property type="protein sequence ID" value="ENSBIXP00000032381.1"/>
        <property type="gene ID" value="ENSBIXG00000014943.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000003">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000160">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
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    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
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    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
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    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="292"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="760"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="793"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="833"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="932"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047282"/>
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        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="999" mass="109321" checksum="FFE7F1FB1D614384" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E227</accession>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 26"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BTB_POZ_BTBD16"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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            <begin position="81"/>
            <end position="202"/>
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            <begin position="380"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00016271"/>
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        <source>
            <dbReference type="Pfam" id="PF21059"/>
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        <source>
            <dbReference type="Pfam" id="PF23998"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030835.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="506" mass="58331" checksum="0F301BCC099D2EF9" modified="2019-09-18" version="1">MKMLNPQKPRLERRIAGSTNRWRFPRQPFSGDLLGLSQMCKAANIDFDEVLKNPDRLCISQIQKIFPENLKNKTIQSREADVILECLGFKWELHQPQLFQSETLAKLYLMGLARGNANPEKESVKILQAQQPGRSREQRPVKKMILSLKINDPLVTREAFATALKNLYMQEVKICLDDVLGVLAAAHILQFGSLFQRCVTVMMSGLTPSTIKNFYLAGCKYKEEPLTTACEKWMEMNLVPLVGTQIHLRKIPKELLHKVLKSPRLFTFSEFDLLKTLLLWVYLQLNNKTQTFPMYQTVLMFFSSFPKNCAFLDRDVGQNLIPLFLCLRLHSITKGKDLEELRHINFFPESWLVRVTANHYHALENGGDMAHVKDLSTQAVRFGLLFNQEYTTHSKVITLYGFFFEIKGIKHDTTSYSFHMQRIRHTDLECASTVYENTPISLRAERLVTYEIRAQTTVDGKWQEFRTNQITQKFGLAEPSCKSHALKIQTVGIPIYASFSFVFSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2A2</accession>
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            <fullName evidence="23">Glutamate receptor</fullName>
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        <name evidence="26" type="primary">GRIA2</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
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        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18 23">Postsynaptic cell membrane</location>
            <topology evidence="18 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027567218.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2E2A2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044733.1">
        <property type="protein sequence ID" value="ENSBIXP00000033325.1"/>
        <property type="gene ID" value="ENSBIXG00000002461.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016124.1">
        <property type="protein sequence ID" value="ENSBIXP00005033068.1"/>
        <property type="gene ID" value="ENSBIXG00005001716.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000156950"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004970">
        <property type="term" value="F:glutamate-gated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="PBP1_iGluR_AMPA_GluR2"/>
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    </dbReference>
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        <property type="entry name" value="PBP2_iGluR_AMPA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="glutamate receptor 2 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Glutamate receptor ionotropic, kainate 2"/>
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    </dbReference>
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        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="Glu/Gly-bd"/>
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        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBPe"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="8 23" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 23" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 23" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 23" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 23" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516457" description="Glutamate receptor" evidence="23">
        <location>
            <begin position="25"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="542"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="815"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="24">
        <location>
            <begin position="415"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="25">
        <location>
            <begin position="425"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="676"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="726"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="21">
        <location>
            <position position="654"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="681"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="773"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="78"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="739"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="883" mass="98848" checksum="3030F3E1102EE2C5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4E5</accession>
    <name>A0A4W2E4E5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex I-B12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase B12 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NDUFB3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB3 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419992.1">
        <property type="nucleotide sequence ID" value="XM_027564191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4E5"/>
    <dbReference type="STRING" id="30522.A0A4W2E4E5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021534.1">
        <property type="protein sequence ID" value="ENSBIXP00000034152.1"/>
        <property type="gene ID" value="ENSBIXG00000017082.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056781.1">
        <property type="protein sequence ID" value="ENSBIXP00005046926.1"/>
        <property type="gene ID" value="ENSBIXG00005031558.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906285"/>
    <dbReference type="CTD" id="4709"/>
    <dbReference type="GeneTree" id="ENSGT00390000010316"/>
    <dbReference type="OMA" id="YMGGFAH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012576">
        <property type="entry name" value="NDUFB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15082:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15082">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B12 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08122">
        <property type="entry name" value="NDUF_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14423" checksum="4C9C86FF5AD210B8" modified="2019-09-18" version="1">MSTSGLLHVTSTGPGISDPGQECLIYPLVSCHMAHGHGHEHGPSKMELPDYKQWKIEGTPLETVQEKLAARGLRDPWGRNEAWRYMGGFANNVSFVGALLKGFKWGFAAFVVAIGAEYYLESQKKDKKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E527</accession>
    <name>A0A4W2E527_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Butyrophilin subfamily 2 member A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BTN2A2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. BTN/MOG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027380479.1">
        <property type="nucleotide sequence ID" value="XM_027524678.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380480.1">
        <property type="nucleotide sequence ID" value="XM_027524679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E527"/>
    <dbReference type="STRING" id="30522.A0A4W2E527"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001798.1">
        <property type="protein sequence ID" value="ENSBIXP00000031208.1"/>
        <property type="gene ID" value="ENSBIXG00000013410.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003807.1">
        <property type="protein sequence ID" value="ENSBIXP00005006733.1"/>
        <property type="gene ID" value="ENSBIXG00005012204.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881610"/>
    <dbReference type="CTD" id="10385"/>
    <dbReference type="GeneTree" id="ENSGT00940000158017"/>
    <dbReference type="OMA" id="AETHHNH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070371">
        <property type="term" value="P:ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046007">
        <property type="term" value="P:negative regulation of activated T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001818">
        <property type="term" value="P:negative regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050860">
        <property type="term" value="P:negative regulation of T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045591">
        <property type="term" value="P:positive regulation of regulatory T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05713">
        <property type="entry name" value="IgV_MOG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15819">
        <property type="entry name" value="SPRY_PRY_BTN1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000208">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037958">
        <property type="entry name" value="SPRY/PRY_BTN1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF139">
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="chain" id="PRO_5044610998" evidence="12">
        <location>
            <begin position="26"/>
            <end position="535"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="250"/>
            <end position="272"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="28"/>
            <end position="146"/>
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            <begin position="313"/>
            <end position="506"/>
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            <begin position="279"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="535" mass="60870" checksum="31C0D47ABF69069F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5A9</accession>
    <name>A0A4W2E5A9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="9">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MAP2K6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="9"/>
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        <property type="nucleotide sequence ID" value="XM_027519120.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2E5A9"/>
    <dbReference type="STRING" id="30522.A0A4W2E5A9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022285.1">
        <property type="protein sequence ID" value="ENSBIXP00000034505.1"/>
        <property type="gene ID" value="ENSBIXG00000002933.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043909.1">
        <property type="protein sequence ID" value="ENSBIXP00005045629.1"/>
        <property type="gene ID" value="ENSBIXG00005029200.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878203"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157836"/>
    <dbReference type="OMA" id="FPYNTWG"/>
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        <property type="component" value="Chromosome 19"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071493">
        <property type="term" value="P:cellular response to UV-B"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0120163">
        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904784">
        <property type="term" value="P:NLRP1 inflammasome complex assembly"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:pyroptotic inflammatory response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:stress-activated MAPK cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="PKc_MKK3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000126">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000158">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF12">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="53"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="334" mass="37578" checksum="39C23E5541719C04" modified="2019-09-18" version="1">MSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIVELGRGAYGVVEKMRHVPSEQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSEEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKATDVASFVKSILGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5S4</accession>
    <name>A0A4W2E5S4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sperm surface protein Sp17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Sperm autoantigenic protein 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SPA17</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. May interact with ROPN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027388525.1">
        <property type="nucleotide sequence ID" value="XM_027532724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5S4"/>
    <dbReference type="SMR" id="A0A4W2E5S4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014681.1">
        <property type="protein sequence ID" value="ENSBIXP00000027057.1"/>
        <property type="gene ID" value="ENSBIXG00000028897.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051729.1">
        <property type="protein sequence ID" value="ENSBIXP00005023131.1"/>
        <property type="gene ID" value="ENSBIXG00005025948.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886496"/>
    <dbReference type="CTD" id="53340"/>
    <dbReference type="GeneTree" id="ENSGT00440000039164"/>
    <dbReference type="OMA" id="STHYRIP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12100">
        <property type="entry name" value="DD_CABYR_SP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000045">
        <property type="entry name" value="Sperm surface protein Sp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000006">
        <property type="entry name" value="Sperm surface protein Sp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047579">
        <property type="entry name" value="DD_CABYR_SP17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012105">
        <property type="entry name" value="Sp17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10699">
        <property type="entry name" value="NEUROMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10699:SF16">
        <property type="entry name" value="SPERM SURFACE PROTEIN SP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016533">
        <property type="entry name" value="Sp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIIa" evidence="8">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="147" mass="16960" checksum="AA1DDB6F7BEA33F7" modified="2019-09-18" version="1">MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDPAEWGAKVDDRFYNNHAFKEQESPEKHEPGKENSQTSVKEKTALESPEEDKDMEENAALKIQAAFRGHLAREEVKKMKSSDLEEEKTEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8A5</accession>
    <name>A0A4W2E8A5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E8A5"/>
    <dbReference type="STRING" id="30522.A0A4W2E8A5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018937.1">
        <property type="protein sequence ID" value="ENSBIXP00000033048.1"/>
        <property type="gene ID" value="ENSBIXG00000002389.1"/>
    </dbReference>
    <dbReference type="OMA" id="QLDQMEI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000014">
        <property type="entry name" value="Complement factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF13">
        <property type="entry name" value="CATHEPSIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="74"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="304" mass="34183" checksum="E8BE12761DE36F4F" modified="2019-09-18" version="1">MKLITAAGEWDCGCLSPHPHPQTPTFTFASSPRGSKSGRRKQGQQLLGSLAGKMWPLLLLVAFLLSPRARAGQIIGGREARPHSRPYMAYIQIRSPVGIKVCGGFLVREDFVMTAAHCLGSQINVILGAHNIRTLESTQQRIPVLRSIPHPGYSQQNKRNDIMLLQLANRAQRNRFVRPVPLPQTQNRLRPGTQCTVAGWGLIGLNMRTDTLQCVQLRVQRDRVCRRRFMLYYGRTQICVGDPRQRKSAFLGDSGGPLVCSNVAQGVVSYGDRMGTPPAVFTRISSFLPWIRRTMRRFQEWRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAE6</accession>
    <name>A0A4W2EAE6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with vasopressin receptors V1bR/AVPR1B (Ki=85 pM), V1aR/AVPR1A (Ki=0.6 nM) and V2R/AVPR2 (Ki=4.9 nM). Interacts with oxytocin receptor (OXTR) (Ki=110 nM).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vasopressin/oxytocin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAE6"/>
    <dbReference type="SMR" id="A0A4W2EAE6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046991.1">
        <property type="protein sequence ID" value="ENSBIXP00000036408.1"/>
        <property type="gene ID" value="ENSBIXG00000019879.1"/>
    </dbReference>
    <dbReference type="OMA" id="CMTEPEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005185">
        <property type="term" value="F:neurohypophyseal hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031894">
        <property type="term" value="F:V1A vasopressin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.9.10:FF:000001">
        <property type="entry name" value="oxytocin-neurophysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.9.10">
        <property type="entry name" value="Neurohypophysial hormone domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000981">
        <property type="entry name" value="Neurhyp_horm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036387">
        <property type="entry name" value="Neurhyp_horm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022423">
        <property type="entry name" value="Neurohypophysial_hormone_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681">
        <property type="entry name" value="NEUROPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681:SF9">
        <property type="entry name" value="VASOPRESSIN-NEUROPHYSIN 2-COPEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00220">
        <property type="entry name" value="Hormone_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00184">
        <property type="entry name" value="Hormone_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001815">
        <property type="entry name" value="Nonapeptide_hormone_precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00831">
        <property type="entry name" value="NEUROPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00003">
        <property type="entry name" value="NH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49606">
        <property type="entry name" value="Neurophysin II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00264">
        <property type="entry name" value="NEUROHYPOPHYS_HORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="8" id="KW-0839">Vasoconstrictor</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021413321" evidence="11">
        <location>
            <begin position="20"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="52"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="59"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001815-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="166" mass="17325" checksum="0441DC255288D8F6" modified="2019-09-18" version="1">MPDATLPACFLSLLAFTSACYFQNCPRGGKRAMSDLELRQCLPCGPGGKGRCFGPSICCGDELGCFVGTAEALRCQEENYLPSPCQSGQKPCGSGGRCAAAGICCNDESCVTEPECREGVGFPRRVRANDRSNATLLDGPSGALLLRLVQLAGAPEPAEPAQPGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBI0</accession>
    <name>A0A4W2EBI0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBI0"/>
    <dbReference type="SMR" id="A0A4W2EBI0"/>
    <dbReference type="STRING" id="30522.A0A4W2EBI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008002.1">
        <property type="protein sequence ID" value="ENSBIXP00000029168.1"/>
        <property type="gene ID" value="ENSBIXG00000000642.1"/>
    </dbReference>
    <dbReference type="OMA" id="ECPVPQG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Datilografo, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein Gfi-1"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000489">
        <property type="entry name" value="Zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein Gfi-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000432">
        <property type="entry name" value="zinc finger protein Gfi-1b isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF419">
        <property type="entry name" value="FI21258P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="305"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="331" mass="37433" checksum="36211532BD32492F" modified="2019-09-18" version="1">MPRSFLVKSKKAHTYHQPRTREDELLWPLALGPVARDQVPSISPVLSSLFPNQRLDWTDLKREPQLELEQSSMTRLASGPEGPAVTSRPQDGDSPSSDSPPFYKPSFSWDTLASSYGHSYRQAPSTMQSAFLERSVSLYGSPLVPSAEPPLDFSLHYSPGMDAYHCIKCSKVFSTPHGLEVHARRSHSGTRPFACNVCGKTFGHSVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCAKGFQRKVDLRRHRESQHNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDJ0</accession>
    <name>A0A4W2EDJ0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-type tyrosine-protein phosphatase delta</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Can bidirectionally induce pre- and post-synaptic differentiation of neurons by mediating interaction with IL1RAP and IL1RAPL1 trans-synaptically. Involved in pre-synaptic differentiation through interaction with SLITRK2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029525.1">
        <property type="protein sequence ID" value="ENSBIXP00000037607.1"/>
        <property type="gene ID" value="ENSBIXG00000021232.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099054">
        <property type="term" value="P:presynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099537">
        <property type="term" value="P:trans-synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd05738">
        <property type="entry name" value="IgI_2_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14628">
        <property type="entry name" value="R-PTP-D-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14624">
        <property type="entry name" value="R-PTPc-D-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000027">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000036">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000066">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000068">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000015">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000023">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000082">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000128">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000181">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000098">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase F isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045905">
        <property type="entry name" value="R-PTP-delta_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF11">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021422028" description="Receptor-type tyrosine-protein phosphatase delta" evidence="20">
        <location>
            <begin position="21"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1267"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="24"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="126"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="236"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="325"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="420"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="518"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="612"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="714"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="823"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="921"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="1357"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1532"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="1644"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1821"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1299"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1310"/>
            <end position="1319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1912" mass="214582" checksum="35F91073F684BCC5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGE7</accession>
    <name>A0A4W2EGE7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome 1 open reading frame 105</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C1orf105</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGE7"/>
    <dbReference type="STRING" id="30522.A0A4W2EGE7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026838.1">
        <property type="protein sequence ID" value="ENSBIXP00000036447.1"/>
        <property type="gene ID" value="ENSBIXG00000019935.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049349.1">
        <property type="protein sequence ID" value="ENSBIXP00005039559.1"/>
        <property type="gene ID" value="ENSBIXG00005003707.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015231"/>
    <dbReference type="OMA" id="RFDKIPW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027845">
        <property type="entry name" value="DUF4548"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39410">
        <property type="entry name" value="RIKEN CDNA 4930558K02 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39410:SF1">
        <property type="entry name" value="RIKEN CDNA 4930558K02 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15081">
        <property type="entry name" value="DUF4548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="184" mass="21100" checksum="1CA8ED0886F398E1" modified="2019-09-18" version="1">MEKRELKVSVPRFHKIPWFSEASFMNKPLVLSLPKRYPHSSATFLVASKKDMNLPILFQVPDILSKAGWNQNDPTLIRNKQLCSTCRGVKMVQPKTLIIPDDRKLSFANIMNHRMMSLHQPKAQTVPKRSRDDILTENVHYRLPILGPRTAVFHRLLSDAYETLQETQLSSLPSKEPVSKAVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EK10</accession>
    <name>A0A4W2EK10_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein naked cuticle homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NKD2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Cell autonomous antagonist of the canonical Wnt signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NKD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EK10"/>
    <dbReference type="STRING" id="30522.A0A4W2EK10"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021197.1">
        <property type="protein sequence ID" value="ENSBIXP00000034003.1"/>
        <property type="gene ID" value="ENSBIXG00000016894.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039140.1">
        <property type="protein sequence ID" value="ENSBIXP00005024042.1"/>
        <property type="gene ID" value="ENSBIXG00005026692.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033589"/>
    <dbReference type="OMA" id="RQEHHGK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040140">
        <property type="entry name" value="Nkd-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611">
        <property type="entry name" value="PROTEIN NAKED CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611:SF1">
        <property type="entry name" value="PROTEIN NAKED CUTICLE HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="350"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="218"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="483" mass="52410" checksum="DAAA490294B83522" modified="2019-09-18" version="1">MGKIQSKHGEARAGGLAGGGGRAPATHCPFPLPAPSGRAAAASAFKRRESPEGDSIVVSAYMGGRRGAEEAERRGCAEHGARDKQELLGGDLREGPFRDNQCPLEVMLPPEKAEACEGPGQLFCVDDGERAASREGARGLGKRRLNVDAVQCDVSVEEDDHQEWTFTLYGFDNSGKATREDMSSLMHTIYEVVDASVHRCSGSSKTLRVKLTVSPEPSSKRKDGPPAAQDREASRCRAEAELSEDPRVADRRLSAHVRRPNADPHPCSERGPYCVDENTERRNHYLDLAGIENYASKFGPGSPPTQARPEPPARASHPQGRSRSQESDVHAAHHRRCPGLAEFALPAAEPLPRALDLPPRPKGPERPFLRSPQGSGRPPGAPSGLRPGRSFSFHPPAPLPQAPLEAHPLPQPPPPPYGHRRCRQRAREGHSPLKAAPGPPGALEQELPPVLLGEAFAGPAVQRHEHHHHHEHHHHHHHHHHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKS3</accession>
    <name>A0A4W2EKS3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">BRCA1-associated RING domain protein 1</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RING-type E3 ubiquitin transferase BARD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">BARD1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homo- and heterodimer. Heterodimer (RING-type zinc finger) with BRCA1. Heterodimer (via ANK repeats and BRCT domains) with CSTF1/CSTF-50. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with UBXN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027422562.1">
        <property type="nucleotide sequence ID" value="XM_027566761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKS3"/>
    <dbReference type="STRING" id="30522.A0A4W2EKS3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024312.1">
        <property type="protein sequence ID" value="ENSBIXP00000035369.1"/>
        <property type="gene ID" value="ENSBIXG00000018640.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051649.1">
        <property type="protein sequence ID" value="ENSBIXP00005042408.1"/>
        <property type="gene ID" value="ENSBIXG00005005782.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907509"/>
    <dbReference type="CTD" id="580"/>
    <dbReference type="GeneTree" id="ENSGT00940000156532"/>
    <dbReference type="OMA" id="LGQCEHV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17734">
        <property type="entry name" value="BRCT_Bard1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17720">
        <property type="entry name" value="BRCT_Bard1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16496">
        <property type="entry name" value="RING-HC_BARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000032">
        <property type="entry name" value="BCL-6 corepressor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000349">
        <property type="entry name" value="BRCA1 associated RING domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000013">
        <property type="entry name" value="BRCA1 associated RING domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000019">
        <property type="entry name" value="BRCA1 associated RING domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039503">
        <property type="entry name" value="BARD1_Znf-RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14835">
        <property type="entry name" value="zf-RING_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="3"/>
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            <end position="81"/>
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            <begin position="284"/>
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            <begin position="323"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055451"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063650"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068947"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00079537"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="773" mass="85842" checksum="29A14AAA86ACA7E3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMN0</accession>
    <name>A0A4W2EMN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum membrane protein complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ER membrane protein complex subunit 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EMC7</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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    <dbReference type="GeneTree" id="ENSGT00390000017490"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0072546">
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
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        <property type="entry name" value="Carb-bd-like_fold"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5044611145" description="Endoplasmic reticulum membrane protein complex subunit 7" evidence="12">
        <location>
            <begin position="23"/>
            <end position="241"/>
        </location>
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            <end position="182"/>
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    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="13">
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044526"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044558"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="241" mass="26502" checksum="F6B9CCE7424E46A5" modified="2019-09-18" version="1">MAATLWAFFSVLLLLLSRDAQSSEVSGSTSDGSGGSGVGTGDRFKIEGRAVVPGVKPQDWISAARVLVDGEEHVGFLKTDGSFVVHDIPSGSYVVEVISPAYRFDPVRVDITSKGKMRARYVNYIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWTDFLMNPMVMMMVLPLLIFVLLPKVVNTSDPDMRREMEQSMNMLNSNHELPDVSEFMTRLFSSKSSGKSSSGSSKTGKSGAGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ENG5</accession>
    <name>A0A4W2ENG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 512</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZNF512</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411588.1">
        <property type="nucleotide sequence ID" value="XM_027555787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ENG5"/>
    <dbReference type="STRING" id="30522.A0A4W2ENG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031116.1">
        <property type="protein sequence ID" value="ENSBIXP00000038292.1"/>
        <property type="gene ID" value="ENSBIXG00000022083.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015787.1">
        <property type="protein sequence ID" value="ENSBIXP00005000629.1"/>
        <property type="gene ID" value="ENSBIXG00005029986.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901440"/>
    <dbReference type="CTD" id="84450"/>
    <dbReference type="GeneTree" id="ENSGT00940000158595"/>
    <dbReference type="OMA" id="SVEEQWY"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Krueppel_C2H2_Zn-finger"/>
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            <begin position="287"/>
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        <location>
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            <end position="130"/>
        </location>
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        <location>
            <begin position="485"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="16">
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            <begin position="495"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="556"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="567" mass="64684" checksum="0A78A259D38140D1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EP54</accession>
    <name>A0A4W2EP54_BOBOX</name>
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        <submittedName>
            <fullName evidence="14">Transmembrane p24 trafficking protein 5</fullName>
        </submittedName>
    </protein>
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        <name evidence="14" type="primary">TMED5</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="4 10">Belongs to the EMP24/GP25L family.</text>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="226" mass="25875" checksum="36E306A97489647E" modified="2019-09-18" version="1">MGDKTWLPFPVVLLAALLLPRAAGFTPSLDSDFTFTLPAGQKECFYQPMPLKASLEIEYQVLDGAGLDIDFHLASPEGKTLVFEQRKSDGVHTIETEVGDYMFCFDNTFSTISEKVIFFELILDNMGEQEQEQEDWKKYITGTDMLDMKLEDILESINSIKSRLSKSGHIQTLLRAFEARDRNIQESNFDRVNFWSMVNLVVMVVVSAIQVYMLKSLFEDKRKSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESH3</accession>
    <name>A0A4W2ESH3_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="gene ID" value="ENSBIXG00000004777.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="RT_POL"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="32"/>
            <end position="300"/>
        </location>
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    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="213"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="341" mass="38859" checksum="7A671BD537DB4B60" modified="2019-09-18" version="1">MNKASGGGGIPVKLCQILKDDAVKVLHSICKKIWKIQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKSLQARLQQYVNRELPDVQAGFRKGRGTRDQIANIRWIVEKAKEFQKNIYFCFIDYAKAFDWVDHNKLWKILKEMGIPEHLTCLLRNLYADQEATVRTGHGTTDWFQIGKGVLQGCILSPCLFNLYAEYTMRNAGLEEAQAGIKIARRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASSPIISWQIDGETVETMADFIFLGSKITADGDCSHEVKRRLLLGRKVIMTNLLLLLSHFSRVQLCATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESI0</accession>
    <name>A0A4W2ESI0_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESI0"/>
    <dbReference type="STRING" id="30522.A0A4W2ESI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049391.1">
        <property type="protein sequence ID" value="ENSBIXP00000039734.1"/>
        <property type="gene ID" value="ENSBIXG00000023682.1"/>
    </dbReference>
    <dbReference type="OMA" id="MAGHSKP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="C1orf162"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37997">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN C1ORF162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37997:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN C1ORF162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="139" mass="14869" checksum="C48C47803DF9AF32" modified="2019-09-18" version="1">MGTQMSKPEPNDKGPGTHPSTALAVTSAPCLSGHPSKEHLVLAFFSGVILTLLLMAFVFLIIKSCRKCHSSPQTLDPPSDHPAKLSSSEEVLTYASMIFKAPEENSNHLTKSVQNAVHLDPVVYAQVKVTNSPCLSSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUA4</accession>
    <name>A0A4W2EUA4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113887693</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027390673.1">
        <property type="nucleotide sequence ID" value="XM_027534872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EUA4"/>
    <dbReference type="SMR" id="A0A4W2EUA4"/>
    <dbReference type="STRING" id="30522.A0A4W2EUA4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036351.1">
        <property type="protein sequence ID" value="ENSBIXP00000040585.1"/>
        <property type="gene ID" value="ENSBIXG00000024640.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000050052.1">
        <property type="protein sequence ID" value="ENSBIXP00000021595.1"/>
        <property type="gene ID" value="ENSBIXG00000024642.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887693"/>
    <dbReference type="OMA" id="ASEAYLX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0164">Citrullination</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="136" mass="15388" checksum="6FD8508EA50A0EEC" modified="2019-09-18" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV50</accession>
    <name>A0A4W2EV50_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Cholinergic receptor nicotinic alpha 2 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CHRNA2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405781.1">
        <property type="nucleotide sequence ID" value="XM_027549980.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405782.1">
        <property type="nucleotide sequence ID" value="XM_027549981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EV50"/>
    <dbReference type="SMR" id="A0A4W2EV50"/>
    <dbReference type="STRING" id="30522.A0A4W2EV50"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036561.1">
        <property type="protein sequence ID" value="ENSBIXP00000040653.1"/>
        <property type="gene ID" value="ENSBIXG00000005035.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050216.1">
        <property type="protein sequence ID" value="ENSBIXP00005020221.1"/>
        <property type="gene ID" value="ENSBIXG00005023343.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897336"/>
    <dbReference type="CTD" id="1135"/>
    <dbReference type="GeneTree" id="ENSGT00940000158299"/>
    <dbReference type="OMA" id="SSYHWLE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19015">
        <property type="entry name" value="LGIC_ECD_nAChR_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000014">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000005">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516481" evidence="18">
        <location>
            <begin position="28"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="36"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="249"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="506" mass="57801" checksum="03EE90B9D302AF45" modified="2019-09-18" version="1">MGPSRLVLPFKTKLGLWWLLLIPAVSSKRAPHTQAEDRLFKHLFRGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPVDFGNITSLRVPSEMIWIPDIVLYNNADGEFVVTHMTKAHLFSSGLLHWAPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWSYDKAKIDLVQMEQTVDLKDYWESGEWAIVNATGTYNTKKYDCCAEVYADVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLISEYLLFTMIFVTLSIVITVFVLNVHHRSASTHNMPHWVRVAFLGCVSRWLLISRPLPPLELRDTPDLKPCPSYHWLETNVDVEEREEEEEEEEGRWVCVGPSSPSVGVYTRGSLHPVASGPRAEAQVPEGGLLLSPRVQKALEGVHYIADHLRAEDADSSVKEDWRYVAMVIDRIFLWLFILVCFLGTVGLFLPPFLAGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVQ5</accession>
    <name>A0A4W2EVQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC113894370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EVQ5"/>
    <dbReference type="STRING" id="30522.A0A4W2EVQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037584.1">
        <property type="protein sequence ID" value="ENSBIXP00000041115.1"/>
        <property type="gene ID" value="ENSBIXG00000005164.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="7">
        <location>
            <begin position="85"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="341" mass="37708" checksum="CD004D5A6EFA1F67" modified="2019-09-18" version="1">MLCRLSVRWLRPRPALQVRVLDPLLARAPAGGAKSSALPVWAVASVSAVGPSGAGGWYEALATSAPVQGAEDVLLFAHTASGLPWWGSILLTTVVLRGAVTLPLAAYQHYILAKVENLQPEIKNIARHLNQEVAVRAHQLGWSKRVARLTYLKTMRRLVSELYVRDNCHPFKATVLVWIQLPMWIFMSVALRNFSTGAAHSEAGFSVQEQLTAGGVLWFHDLTALDSTWILPVSVGVINLLIVEIFALQKLGKSRFQTYITHFVRAVSVLMIPVAATVPSSIVLYWLCSSFMGLSQNLLLRSPGFRQLCRIPLTKLDSDTPYKDLLAAFHSKFISRKSRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EY10</accession>
    <name>A0A4W2EY10_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <source>
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        </source>
    </evidence>
    <sequence length="570" mass="64441" checksum="425196EE0D41BD4A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F061</accession>
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        <recommendedName>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000738">
        <property type="term" value="P:positive regulation of stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000008">
        <property type="entry name" value="tumor-associated calcium signal transducer 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049420">
        <property type="entry name" value="EPCAM-Trop-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043406">
        <property type="entry name" value="EPCAM/Trop-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041630">
        <property type="entry name" value="EpCAM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14168">
        <property type="entry name" value="TUMOR-ASSOCIATED CALCIUM SIGNAL TRANSDUCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14168:SF5">
        <property type="entry name" value="TUMOR-ASSOCIATED CALCIUM SIGNAL TRANSDUCER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21283">
        <property type="entry name" value="EPCAM-Trop-2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18635">
        <property type="entry name" value="EpCAM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021268210" description="Tumor-associated calcium signal transducer 2" evidence="13">
        <location>
            <begin position="27"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="14">
        <location>
            <begin position="66"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="319" mass="35442" checksum="A9DAA9D9AC5655C5" modified="2019-09-18" version="1">MARGPGLALLSPWLLMLAAVIGHAAAQNQCVCPTNKMTVCDERDPSGRCQCRVLGSNQAVNCSTLTSKCLLLKARMSASHSGRALVRPSEHAIVDNDGLYDPDCDQQGRFKARQCNQTSVCWCVNSVGVRRTDKGDLSLKCDEVVRTYHILIDLRHRPDAPAFNHSDLDAELRQLFQQRYLLQPKFVSAVHYEHPTIQIELRQNSSQKVAGEVDIGDAAYYFERDVKGESLFLGPRDLNLRVGGQPLVLEQMLIYYLDEKPPQFSMKRLTSGLIAVVVVVVVAVVAGVAVLVITSRRKSGKYKKVEIKELGEIRQKPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2T2</accession>
    <name>A0A4W2F2T2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113896645</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F2T2"/>
    <dbReference type="STRING" id="30522.A0A4W2F2T2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042659.1">
        <property type="protein sequence ID" value="ENSBIXP00000043719.1"/>
        <property type="gene ID" value="ENSBIXG00000027867.1"/>
    </dbReference>
    <dbReference type="OMA" id="IIMAIMK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="34817" checksum="84CABFAF7B14858D" modified="2019-09-18" version="1">MEPDNQTNSSEFILLGLSEKPEQETLLFALFLCMYVVTVVGNLLIILAISTDFHLHTPMYFFLANLSLVDFCLATDTVPKMLVSIQIKSNKSISYSCCLTQMYFFHFFGIMDSVLIAVMAYDRFVAICHPLHYTSIMSPRLCGLLVGGPWVFSGFISLTHILLMARLVFCGNIELPHYFCDLTPLLRLSCTDTSVNKIFVLIVAGMVIATPFICILASYAHIIVAIVKVPSAGGRKKTFSTCSSHLSVVVLFYGTTIGVYLCPSSVRTAMKEKASAVMYTAVTPMLNPFIYSLRNRDLKGALMKLVNRKITSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FA01</accession>
    <name>A0A4W2FA01_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Integrator complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">INTS3</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388699.1">
        <property type="nucleotide sequence ID" value="XM_027532898.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388706.1">
        <property type="nucleotide sequence ID" value="XM_027532905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FA01"/>
    <dbReference type="SMR" id="A0A4W2FA01"/>
    <dbReference type="STRING" id="30522.A0A4W2FA01"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039290.1">
        <property type="protein sequence ID" value="ENSBIXP00000041900.1"/>
        <property type="gene ID" value="ENSBIXG00000007730.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012831.1">
        <property type="protein sequence ID" value="ENSBIXP00005002152.1"/>
        <property type="gene ID" value="ENSBIXG00005008552.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886557"/>
    <dbReference type="CTD" id="65123"/>
    <dbReference type="GeneTree" id="ENSGT00390000014184"/>
    <dbReference type="OMA" id="FEQYCLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160232">
        <property type="term" value="C:INTAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160240">
        <property type="term" value="P:RNA polymerase II transcription initiation surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056518">
        <property type="entry name" value="HEAT_Ints3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045334">
        <property type="entry name" value="INTS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019333">
        <property type="entry name" value="INTS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587:SF7">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24566">
        <property type="entry name" value="HEAT_Ints3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10189">
        <property type="entry name" value="Ints3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 3 N-terminal" evidence="7">
        <location>
            <begin position="83"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Ints3-like C-terminal" evidence="8">
        <location>
            <begin position="580"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="974"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1005"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1040" mass="117887" checksum="CD7D883D538B411E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FGE9</accession>
    <name>A0A4W2FGE9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Whirlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">WHRN</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in hearing and vision as member of the USH2 complex. Necessary for elongation and maintenance of inner and outer hair cell stereocilia in the organ of Corti in the inner ear. Involved in the maintenance of the hair bundle ankle region, which connects stereocilia in cochlear hair cells of the inner ear. In retina photoreceptors, required for the maintenance of periciliary membrane complex that seems to play a role in regulating intracellular protein transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms homooligomers. Interacts (via C-terminal PDZ domain) with MYO15A; this interaction is necessary for localization of WHRN to stereocilia tips. Interacts (via C-terminal PDZ domain) with MPP1/p55. Interacts with LRRC4C/NGL1. Interacts with MYO7A. Interacts with RPGR. Interacts with EPS8. Interacts with CASK. Interacts with CIB2. Component of USH2 complex, composed of ADGRV1, PDZD7, USH2A and WHRN. Interacts (via PDZ domains) with PDZD7; the interaction is direct. Interacts (via N-terminal PDZ domain) with USH2A (via cytoplasmic region). Interacts with ADGRV1/MASS1 (via cytoplasmic region).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027406413.1">
        <property type="nucleotide sequence ID" value="XM_027550612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FGE9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048786.1">
        <property type="protein sequence ID" value="ENSBIXP00000019091.1"/>
        <property type="gene ID" value="ENSBIXG00000004452.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009059.1">
        <property type="protein sequence ID" value="ENSBIXP00005004069.1"/>
        <property type="gene ID" value="ENSBIXG00005010093.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897708"/>
    <dbReference type="CTD" id="25861"/>
    <dbReference type="GeneTree" id="ENSGT00950000183002"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07356">
        <property type="entry name" value="HN_L-whirlin_R1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07357">
        <property type="entry name" value="HN_L-whirlin_R2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06740">
        <property type="entry name" value="PDZ1_FL-whirlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06741">
        <property type="entry name" value="PDZ2_FL-whirlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06742">
        <property type="entry name" value="PDZ3_FL-whirlin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000002">
        <property type="entry name" value="Whirlin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000003">
        <property type="entry name" value="Whirlin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000079">
        <property type="entry name" value="Whirlin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000087">
        <property type="entry name" value="Whirlin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000111">
        <property type="entry name" value="Whirlin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047056">
        <property type="entry name" value="Whirlin_HN-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033028">
        <property type="entry name" value="Whirlin_HN-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF37">
        <property type="entry name" value="WHIRLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1009">Hearing</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="140"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="279"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="816"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="502"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="552"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="676"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="742"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="743"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="789"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="907" mass="96473" checksum="2BE01367F9A3503C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FGY1</accession>
    <name>A0A4W2FGY1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FGY1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037826.1">
        <property type="protein sequence ID" value="ENSBIXP00000041226.1"/>
        <property type="gene ID" value="ENSBIXG00000025411.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009452.1">
        <property type="protein sequence ID" value="ENSBIXP00005003883.1"/>
        <property type="gene ID" value="ENSBIXG00005009914.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000190735"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="93" mass="9884" checksum="EF4E72649EB6A252" modified="2019-09-18" version="1">MSTSSLSFFAGPCTPARSPGTAGRSTGPEERSYSSMCFRTAKGPEGRFKSGFSGSVRRGRGRPCPGLLACVSSAQERGRCTAILTVTFLRMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FMG5</accession>
    <name>A0A4W2FMG5_BOBOX</name>
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        <submittedName>
            <fullName evidence="11">SHOX homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SHOX2</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_027542682.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2FMG5"/>
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        <property type="gene ID" value="ENSBIXG00000025440.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004072.1">
        <property type="protein sequence ID" value="ENSBIXP00005006597.1"/>
        <property type="gene ID" value="ENSBIXG00005012090.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893051"/>
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    <dbReference type="GeneTree" id="ENSGT00940000154287"/>
    <dbReference type="OMA" id="DSHCNMP"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Short stature homeobox 2"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="HD"/>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR052631">
        <property type="entry name" value="Paired_homeobox_Bicoid"/>
    </dbReference>
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        <property type="entry name" value="SHORT STATURE HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46255:SF1">
        <property type="entry name" value="SHORT STATURE HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="138"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="10">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="140"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="26"/>
            <end position="139"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
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            <end position="109"/>
        </location>
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        <location>
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            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="331" mass="34923" checksum="E526D0EF0071AFCC" modified="2019-09-18" version="1">MEELTAFVSKSFDQKVKEKKEAITYREVLESGPLRGAKEPSGCSEPGRDDRSSPAVRAAGGGGGGGGGGGGGGAGGGGGGGAGGGAGGGGRSPVRELDMGAAERSREPGSPRLTEVSPELKDRKEDAKGMEDEGQTKIKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQLHKGVLIGAASQFEACRVAPYVNVGALRMPFQQDSHCNVTPLSFQVQAQLQLDSAVAHAHHHLHPHLAAHAPYMMFPAPPFGLPLATLAADSASAASVVAAAAAAKTTSKNSSIADLRLKAKKHAAALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FUN3</accession>
    <name>A0A4W2FUN3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sterile alpha motif domain containing 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SAMD4B</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMAUG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371652.1">
        <property type="nucleotide sequence ID" value="XM_027515851.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371653.1">
        <property type="nucleotide sequence ID" value="XM_027515852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FUN3"/>
    <dbReference type="STRING" id="30522.A0A4W2FUN3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045010.1">
        <property type="protein sequence ID" value="ENSBIXP00000011249.1"/>
        <property type="gene ID" value="ENSBIXG00000016553.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044751.1">
        <property type="protein sequence ID" value="ENSBIXP00005009552.1"/>
        <property type="gene ID" value="ENSBIXG00005014450.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876526"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159702"/>
    <dbReference type="OMA" id="PFGDHAP"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein Smaug homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein Smaug homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.170:FF:000002">
        <property type="entry name" value="Protein Smaug homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.170">
        <property type="entry name" value="Smaug, PHAT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHAT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058599">
        <property type="entry name" value="PHAT_Smg/ZCCHC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
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        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
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        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037634">
        <property type="entry name" value="Smaug_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF9">
        <property type="entry name" value="PROTEIN SMAUG HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26034">
        <property type="entry name" value="PHAT_SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="296"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="406"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="606"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="695" mass="75506" checksum="7D7F5D1CA5E40CA6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G0X7</accession>
    <name>A0A4W2G0X7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="2 11">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ASNS</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2 11"/>
    <dbReference type="RefSeq" id="XP_027395890.1">
        <property type="nucleotide sequence ID" value="XM_027540089.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395891.1">
        <property type="nucleotide sequence ID" value="XM_027540090.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395892.1">
        <property type="nucleotide sequence ID" value="XM_027540091.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395894.1">
        <property type="nucleotide sequence ID" value="XM_027540093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G0X7"/>
    <dbReference type="SMR" id="A0A4W2G0X7"/>
    <dbReference type="STRING" id="30522.A0A4W2G0X7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038034.1">
        <property type="protein sequence ID" value="ENSBIXP00000022758.1"/>
        <property type="gene ID" value="ENSBIXG00000025558.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021142.1">
        <property type="protein sequence ID" value="ENSBIXP00005011994.1"/>
        <property type="gene ID" value="ENSBIXG00005016374.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891701"/>
    <dbReference type="CTD" id="440"/>
    <dbReference type="GeneTree" id="ENSGT00390000001994"/>
    <dbReference type="OMA" id="GIVCAFD"/>
    <dbReference type="UniPathway" id="UPA00134">
        <property type="reaction ID" value="UER00195"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070981">
        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045931">
        <property type="term" value="P:positive regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000090">
        <property type="entry name" value="asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000039">
        <property type="entry name" value="Asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF23">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="15">
        <location>
            <begin position="2"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="12">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="363"/>
            <end position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="14">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="561" mass="64220" checksum="71379296556DB3D1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G4L9</accession>
    <name>A0A4W2G4L9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Inhibitor of PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PP1 inhibitor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2G4L9"/>
    <dbReference type="STRING" id="30522.A0A4W2G4L9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043988.1">
        <property type="protein sequence ID" value="ENSBIXP00000009178.1"/>
        <property type="gene ID" value="ENSBIXG00000014893.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022418.1">
        <property type="protein sequence ID" value="ENSBIXP00005012843.1"/>
        <property type="gene ID" value="ENSBIXG00005002761.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182985"/>
    <dbReference type="OMA" id="RHQGNVR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.220:FF:000001">
        <property type="entry name" value="Phosphatase 1, regulatory (Inhibitor) subunit 14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.220">
        <property type="entry name" value="CPI-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008025">
        <property type="entry name" value="CPI-17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036658">
        <property type="entry name" value="CPI-17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16188">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 INHIBITOR POTENTIATED BY PROTEIN KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16188:SF5">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05361">
        <property type="entry name" value="PP1_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81790">
        <property type="entry name" value="Myosin phosphatase inhibitor 17kDa protein, CPI-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 5" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="148" mass="15987" checksum="7BFEA4FC8FF5D6DF" modified="2019-09-18" version="1">MEGPDAHVTLEGRVGGPGLQTGLGSGGPGPRLYFQSHPGAAGEGLGGADDGGPVRHQGNVRVKYDGKELLKRLSLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDTWAARVKELLVDCYKPTEPAGQDPGHAEAEHTPEEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GE24</accession>
    <name>A0A4W2GE24_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cortistatin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CORT</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the somatostatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GE24"/>
    <dbReference type="STRING" id="30522.A0A4W2GE24"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046828.1">
        <property type="protein sequence ID" value="ENSBIXP00000015071.1"/>
        <property type="gene ID" value="ENSBIXG00000003516.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027987.1">
        <property type="protein sequence ID" value="ENSBIXP00005016571.1"/>
        <property type="gene ID" value="ENSBIXG00005020193.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111752"/>
    <dbReference type="OMA" id="TRCKNFF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004250">
        <property type="entry name" value="Somatostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018142">
        <property type="entry name" value="Somatostatin/Cortistatin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558:SF1">
        <property type="entry name" value="CORTISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558">
        <property type="entry name" value="SOMATOSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03002">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001814">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611614" evidence="9">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Somatostatin/Cortistatin C-terminal" evidence="10">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001814-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="105" mass="11601" checksum="D4745F129F9B61C7" modified="2019-09-18" version="1">MLPLCLLLPLLLLPSGATTALSPEGGLAGHESGHMQEVAEIKTNSLLTFLAWWYEWASQARAVPFVGGEAREVSKRQEGAPLHQSTRQDKTPCKNFFWKTFSSCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GHE5</accession>
    <name>A0A4W2GHE5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Centromere protein R</fullName>
            <shortName evidence="1">CENP-R</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nuclear receptor-interacting factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ITGB3BP</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription coregulator that can have both coactivator and corepressor functions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GHE5"/>
    <dbReference type="STRING" id="30522.A0A4W2GHE5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041429.1">
        <property type="protein sequence ID" value="ENSBIXP00000043075.1"/>
        <property type="gene ID" value="ENSBIXG00000027123.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030384.1">
        <property type="protein sequence ID" value="ENSBIXP00005017848.1"/>
        <property type="gene ID" value="ENSBIXG00005021312.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004336"/>
    <dbReference type="OMA" id="FMVVFSK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009601">
        <property type="entry name" value="CENP-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15581">
        <property type="entry name" value="CENTROMERE PROTEIN R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15581:SF0">
        <property type="entry name" value="CENTROMERE PROTEIN R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06729">
        <property type="entry name" value="CENP-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011860">
        <property type="entry name" value="NRIF3_coact_rcpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0137">Centromere</keyword>
    <keyword evidence="1" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="178" mass="20111" checksum="5B7F4AC8A97CAC79" modified="2019-09-18" version="1">MPVKRSLKLDGMLKADSFGSPKITRKKSAADYSPTTGTCQMSPVASPTSSKEQENRNGPSNGKRENLNHLSINKRMESTMQDNEFMVLLSKVEKSSEEFMEIMQNLSSIQALKGSKELENLIGIPHASCVFKREMQKTKKLMTKVIKQKLFKKKNSGLPNKELYDFDSCEFLKTILNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GVL7</accession>
    <name>A0A4W2GVL7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit G</fullName>
            <shortName evidence="6">eIF3g</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 RNA-binding subunit</fullName>
            <shortName evidence="6">eIF-3 RNA-binding subunit</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF-3-delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p42</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p44</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">EIF3G</name>
        <name evidence="6" type="synonym">EIF3S4</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts (via C-terminus) with AIFM1 (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
        <text evidence="6">Colocalizes with AIFM1 in the nucleus and perinuclear region.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Phosphorylated. Phosphorylation is enhanced upon serum stimulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit G family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027403593.1">
        <property type="nucleotide sequence ID" value="XM_027547792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GVL7"/>
    <dbReference type="SMR" id="A0A4W2GVL7"/>
    <dbReference type="STRING" id="30522.A0A4W2GVL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052569.1">
        <property type="protein sequence ID" value="ENSBIXP00000025913.1"/>
        <property type="gene ID" value="ENSBIXG00000028017.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038063.1">
        <property type="protein sequence ID" value="ENSBIXP00005023304.1"/>
        <property type="gene ID" value="ENSBIXG00005026069.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895934"/>
    <dbReference type="CTD" id="8666"/>
    <dbReference type="GeneTree" id="ENSGT00510000047802"/>
    <dbReference type="OMA" id="ICQGDHF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075525">
        <property type="term" value="P:viral translational termination-reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12933">
        <property type="entry name" value="eIF3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12408">
        <property type="entry name" value="RRM_eIF3G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000194">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03006">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034240">
        <property type="entry name" value="eIF3G_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="239"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="320" mass="35611" checksum="7D7226FEDE9D6FBB" modified="2019-09-18" version="1">MPTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLATGDTSPEPELLPGAPLPPPKEVINGNIKTVTEYKIDEDGKKFKIVRTFRIETRKASKAVARRKNWKKFGNSEFDPPGPNVATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRICKGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSLRDGASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKPSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GXF1</accession>
    <name>A0A4W2GXF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glucose-6-phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">G6PC3</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glucose-6-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.9" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027372927.1">
        <property type="nucleotide sequence ID" value="XM_027517126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GXF1"/>
    <dbReference type="SMR" id="A0A4W2GXF1"/>
    <dbReference type="STRING" id="30522.A0A4W2GXF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019707.1">
        <property type="protein sequence ID" value="ENSBIXP00000010718.1"/>
        <property type="gene ID" value="ENSBIXG00000016153.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039033.1">
        <property type="protein sequence ID" value="ENSBIXP00005023966.1"/>
        <property type="gene ID" value="ENSBIXG00005004552.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877224"/>
    <dbReference type="CTD" id="92579"/>
    <dbReference type="GeneTree" id="ENSGT00950000183150"/>
    <dbReference type="OMA" id="KKWCSRA"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004346">
        <property type="term" value="F:glucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015760">
        <property type="term" value="P:glucose-6-phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03381">
        <property type="entry name" value="PAP2_glucose_6_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000018">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016275">
        <property type="entry name" value="Glucose-6-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591:SF2">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000905">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="15">
        <location>
            <begin position="53"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="346" mass="38742" checksum="EBFB51839598E363" modified="2019-09-18" version="1">MESTLGAGIAMAEALQNQLPWLENVWLWVTFLGDPKSLFLFYFPAAYYASRRVGIAVLWISLITEWLNLVFKWFLFGDRPFWWVHESGYYSQAPAQVHQFPSSCETGPGSPSGHCMITGAALWPIMTAVSSQMATRAHSRWVRVIPSLAYCTFLLAVGLSRVFLLAHFPHQVLAGLITGAVLGWLMTPQVPMERELSFYGLTSLALLLGASLIYWTLFTLGLDLSWSINLASKWCERPEWVHLDSRPFASLSRDSGAALGLGIALHSPCYAQVRRAHLGYGQKLVCLVLAMGLLGPLNWLGYPPQISLFYIFNFLKYTLWPCLVLALVPWLVHMFSAQEAPPIRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H9X6</accession>
    <name>A0A4W2H9X6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ELOF1</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Factor involved in transcription-coupled nucleotide excision repair (TC-NER), a mechanism that rapidly removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Acts as a key adapter required to anchor TC-NER factors to RNA polymerase II: stably positions UVSSA and the DCX(ERCC8) complex (also named CSA complex) on arrested RNA polymerase II, leading to neddylation and activation of the DCX(ERCC8) complex and ubiquitination of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H9X6"/>
    <dbReference type="STRING" id="30522.A0A4W2H9X6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043399.1">
        <property type="protein sequence ID" value="ENSBIXP00000044217.1"/>
        <property type="gene ID" value="ENSBIXG00000028403.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054008.1">
        <property type="protein sequence ID" value="ENSBIXP00005026999.1"/>
        <property type="gene ID" value="ENSBIXG00005005151.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000053"/>
    <dbReference type="OMA" id="CLDANKK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000002">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="90" mass="10198" checksum="EBE8DD723CAFD749" modified="2019-09-18" version="1">VVHSPTDMGRRKSKRKPPPKKKMTGTLETQFTCPFCNHEKSCDVKMDRARNTGVISCTVCLEEFQTPITYLSEPVDVYSDWIDACEAANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HF16</accession>
    <name>A0A4W2HF16_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ephrin-A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EFNA1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HF16"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039779.1">
        <property type="protein sequence ID" value="ENSBIXP00000042150.1"/>
        <property type="gene ID" value="ENSBIXG00000026262.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006562.1">
        <property type="protein sequence ID" value="ENSBIXP00005030854.1"/>
        <property type="gene ID" value="ENSBIXG00005001012.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159919"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000017">
        <property type="entry name" value="ephrin-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF19">
        <property type="entry name" value="EPHRIN-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611863" description="Ephrin-A1" evidence="13">
        <location>
            <begin position="19"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="14">
        <location>
            <begin position="18"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="183" mass="21269" checksum="56332AFFC5ABA579" modified="2019-09-18" version="1">MEFFWASLLGLCCSLAAANRHTVFWNSSNPKFWNEDYTVHVRIDDYLDIICPHYEDNSVPDAAMEQYTLYLVEHEQYQLCQPQPKDHARWFCKSPKAKHGPEKLSEKFHRFTGFTLSKDFKEGHSYYYISHSPQAHPNAQEKRLPADDPEVQVLHSIGHSAAPRLFPLAWAVLLLPFLLLQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HGE7</accession>
    <name>A0A4W2HGE7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPL13</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027369584.1">
        <property type="nucleotide sequence ID" value="XM_027513783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HGE7"/>
    <dbReference type="SMR" id="A0A4W2HGE7"/>
    <dbReference type="STRING" id="30522.A0A4W2HGE7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016316.1">
        <property type="protein sequence ID" value="ENSBIXP00000008571.1"/>
        <property type="gene ID" value="ENSBIXG00000014375.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004995.1">
        <property type="protein sequence ID" value="ENSBIXP00005031345.1"/>
        <property type="gene ID" value="ENSBIXG00005011663.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875414"/>
    <dbReference type="CTD" id="6137"/>
    <dbReference type="GeneTree" id="ENSGT00390000007818"/>
    <dbReference type="OMA" id="IQKNHFR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="211" mass="24292" checksum="571567F812A67EED" modified="2019-09-18" version="1">MAPSRNGMILKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPASGPLRPVVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPRRRNKCTESLQANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPIRNVYKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HI35</accession>
    <name>A0A4W2HI35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PTB domain-containing engulfment adapter protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cell death protein 6 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">PTB domain adapter protein CED-6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Protein GULP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GULP1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ced-6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HI35"/>
    <dbReference type="STRING" id="30522.A0A4W2HI35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052820.1">
        <property type="protein sequence ID" value="ENSBIXP00000044186.1"/>
        <property type="gene ID" value="ENSBIXG00000028392.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010706.1">
        <property type="protein sequence ID" value="ENSBIXP00005029474.1"/>
        <property type="gene ID" value="ENSBIXG00005009487.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156186"/>
    <dbReference type="OMA" id="XFARHIK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01273">
        <property type="entry name" value="PTB_CED-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000118">
        <property type="entry name" value="GULP PTB domain containing engulfment adaptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF78">
        <property type="entry name" value="PTB DOMAIN-CONTAINING ENGULFMENT ADAPTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="11">
        <location>
            <begin position="27"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="256"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="387" mass="43691" checksum="5A85DDFC05D48CB9" modified="2019-09-18" version="1">MNRAFSRKKDKTWMHTPEALSKHYIPYNAKFLGSTEVEQPKGTEVVRDAVRKLKFARHIKKSEGQKIPKVELQISIYGVKILEPKTKEVQHNCQLHRISFCADDKTDKRIFTFICKDSESNKHLCYVFDSEKCAEEITLTIGQAFDLAYRKFLESGGKDVETRKQIAGLQKRSSRSSRCWIQDLETENMELKNKVQDLENQLRITHVSTPPLLHKMSDHEQHGFQRCSPSFWHSTDDSSPCLNISSITVTPVNSPDSRLSLGLLIPPPSKRGLPKPDSDNSIPRPHAGSVTPKSPSTDIFDMIPFSPVSHQSSIPTHNGMQPPPVPSRSNEIKRDLFGAEPFDPFNCGAGDFPPDIQSKLDEMQEGFKMGLTLEGTVFCLDPLDSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HM22</accession>
    <name>A0A4W2HM22_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 307</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ZKSCAN4</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027380478.1">
        <property type="nucleotide sequence ID" value="XM_027524677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HM22"/>
    <dbReference type="STRING" id="30522.A0A4W2HM22"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002028.1">
        <property type="protein sequence ID" value="ENSBIXP00000031130.1"/>
        <property type="gene ID" value="ENSBIXG00000013316.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001864.1">
        <property type="protein sequence ID" value="ENSBIXP00005032387.1"/>
        <property type="gene ID" value="ENSBIXG00005012892.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881609"/>
    <dbReference type="CTD" id="387032"/>
    <dbReference type="GeneTree" id="ENSGT00940000159644"/>
    <dbReference type="OMA" id="KESQWEN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001178">
        <property type="entry name" value="zinc finger protein 287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000642">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001214">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001333">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001518">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN 501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="19">
        <location>
            <begin position="53"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00079518"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="543" mass="61150" checksum="7BF4769F72B71800" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HNX9</accession>
    <name>A0A4W2HNX9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Developmental pluripotency-associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC113893621</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027399322.1">
        <property type="nucleotide sequence ID" value="XM_027543521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HNX9"/>
    <dbReference type="STRING" id="30522.A0A4W2HNX9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044139.1">
        <property type="protein sequence ID" value="ENSBIXP00000009438.1"/>
        <property type="gene ID" value="ENSBIXG00000007793.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016301.1">
        <property type="protein sequence ID" value="ENSBIXP00005033054.1"/>
        <property type="gene ID" value="ENSBIXG00005013707.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893621"/>
    <dbReference type="GeneTree" id="ENSGT00800000124303"/>
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        <property type="component" value="Chromosome 5"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:epigenetic programing of female pronucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Dppa3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31577:SF2">
        <property type="entry name" value="DEVELOPMENTAL PLURIPOTENCY-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31577">
        <property type="entry name" value="DEVELOPMENTAL PLURIPOTENCY-ASSOCIATED PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15549">
        <property type="entry name" value="PGC7_Stella"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="163" mass="19078" checksum="C7EFCF8D91FC4D77" modified="2019-09-18" version="1">MDSSEDNPTWTLESLKTSIDDASQAMQVATQLSEMLATNLSNLTLNPSIKLPYLPEYPSQLTGQLPSEKTPHRRRGVRTVLSERRYRMQKLIESLRLRYAKGIPRSDSQRQLQQQEDTEIRSRVRRFQCTCSYCQFKRNPSDDNYENYYNTTYSNYAMESNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IJ50</accession>
    <name>A0A4W2IJ50_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial tRNA methylthiotransferase CDK5RAP1</fullName>
            <ecNumber evidence="8">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CDK5 activator-binding protein C42</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CDK5 regulatory subunit-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CDK5RAP1</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:37068"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2IJ50"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029617.1">
        <property type="protein sequence ID" value="ENSBIXP00000037654.1"/>
        <property type="gene ID" value="ENSBIXG00000021256.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053117.1">
        <property type="protein sequence ID" value="ENSBIXP00005044232.1"/>
        <property type="gene ID" value="ENSBIXG00005028076.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160361"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035597">
        <property type="term" value="F:tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
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        <location>
            <begin position="246"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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    <sequence length="585" mass="66011" checksum="ED3D6B35FE876067" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
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                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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    </gene>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQ40"/>
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    </dbReference>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="269"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="453" mass="52923" checksum="E8F37451FBD21A81" modified="2019-09-18" version="1">MDSEIPEAFQKELTCLVCLNFLLDPVTIGCGHSFCRSCLCLFWEQAKVPASCPVCRQRSEQTSFKTNFLLKNLVSTVRKASFRQFLKCEEHLCATHKQKKTIFCEANKSLLCLVCSQGQEHKTHRHCLAEEAAEESWEKLAKQMTSLWEKIQETERNLKKSSRGTDPWMFYVYRHGDMIRRTYQMAPPFLPEEEDYYVESIVKEGQNIYNQLRRRQEEIIRKKRELREIHKELIQMCSKPDVELLQELEDKLKWSESAQLHVPQPLLPELPARPMSGLMDWLNHFRVNFSLSNEVSSQHIRLFDDARRLTYEHDSLHASLDGGTLKYFASWGDQSFTSGKQYWEMLVDRSWDWAVGVCKDSWIRKDDGILDTSNNDNFLLVCVKQDDHYQLWTTAPTTPLYIERPLGRVGVFLDCDSGSISFVDVAKRTLLWRYDDGVCTFPVRPFFCTGHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQY3</accession>
    <name>A0A4W2BQY3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQY3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014373.1">
        <property type="protein sequence ID" value="ENSBIXP00000001270.1"/>
        <property type="gene ID" value="ENSBIXG00000028738.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="365" mass="42234" checksum="3A44E132AA7E55C3" modified="2019-09-18" version="1">MGSIKDRNGMDLIETEDIKKRWQEYTELYKKDLHDQDNHDGVITHLEPDILECEVKWAFGSITTHKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFVPVPKKLNAKECSNYHTIALISHGSRVTLKILQARLQYMNCELPDVQACFRKGRGTRDQIDNICWIIEKAREFQKNIYFCFIDYAKAFDCVDHDKLWKILQEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQGRILSPCLFNLYAEYIMRNAGLDEAQARIKIARRNINNRRYTDDTTVMAESEDELKSLLMKVKEESEKFGLKLNIWETKIMASGPITSWQIDGETMETVTDYFGGLQNHCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRA0</accession>
    <name>A0A4W2BRA0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Amyloid beta precursor like protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Amyloid beta (A4) precursor-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Amyloid protein homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Amyloid-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">CDEI box-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sperm membrane protein YWK-II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">APLP2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with CPEB1. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif. Interacts (via cytoplasmic domain) with APBB2/FE65L. Interacts (via intracellular domain) with APBB3/FE65L2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRA0"/>
    <dbReference type="STRING" id="30522.A0A4W2BRA0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013441.1">
        <property type="protein sequence ID" value="ENSBIXP00000001686.1"/>
        <property type="gene ID" value="ENSBIXG00000006865.1"/>
    </dbReference>
    <dbReference type="OMA" id="PYRILHA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22607">
        <property type="entry name" value="Kunitz_ABPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000003">
        <property type="entry name" value="amyloid-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF14">
        <property type="entry name" value="AMYLOID BETA PRECURSOR LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
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    <sequence length="770" mass="87234" checksum="1A2569DAFB200AAA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSA1</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="531" mass="57921" checksum="AF9FC4732C01EC16" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSW7</accession>
    <name>A0A4W2BSW7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSW7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012766.1">
        <property type="protein sequence ID" value="ENSBIXP00000002003.1"/>
        <property type="gene ID" value="ENSBIXG00000008545.1"/>
    </dbReference>
    <dbReference type="OMA" id="GGACICI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03165">
        <property type="entry name" value="NET-5_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000006">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF40">
        <property type="entry name" value="TETRASPANIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="238" mass="26166" checksum="5E4AF74A5DF6DD93" modified="2019-09-18" version="1">MARGCLQGVKYLMFAFNLLFWLGGCGILGVGIWLAATQGNFATLSSSFPSLSAANLLIVTGAFVMAIGFVGCIGAIKENKCLLLTFFVALLLVFLLEACITILFFAYTDKIDRYAQRDLKKGLHLYGTPGNVGLTNAWSIIQTDFRCCGVSNYTDWFEVYNATRVPDSCCLEFSESYGLHAPGTWWKAPCYETVKMWLQENLLAVGVFGLCTALVQILGLTFAMTMYCQVVRADAYCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTF3</accession>
    <name>A0A4W2BTF3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">SDHAF2</name>
        <name evidence="4" type="synonym">PGL2</name>
        <name evidence="4" type="synonym">SDH5</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SDHA of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with SDHA within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388298.1">
        <property type="nucleotide sequence ID" value="XM_027532497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTF3"/>
    <dbReference type="SMR" id="A0A4W2BTF3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012384.1">
        <property type="protein sequence ID" value="ENSBIXP00000002210.1"/>
        <property type="gene ID" value="ENSBIXG00000008689.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010064.1">
        <property type="protein sequence ID" value="ENSBIXP00005003566.1"/>
        <property type="gene ID" value="ENSBIXG00005007414.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886347"/>
    <dbReference type="CTD" id="54949"/>
    <dbReference type="GeneTree" id="ENSGT00390000001149"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010719">
        <property type="term" value="P:negative regulation of epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="166" mass="19604" checksum="D6CE06E30DD37519" modified="2019-09-18" version="1">MAVVAVFPALARMLAVSRRRLVSPSLSMTSCRRCYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMTLLRDFAKNKNKEQRLRAPDLEYLFEKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUJ2</accession>
    <name>A0A4W2BUJ2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T-cell surface glycoprotein CD3 epsilon chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CD3E</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6. Interacts (via Proline-rich sequence) with NCK1; the interaction is ligand dependent but independent of tyrosine kinase activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUJ2"/>
    <dbReference type="STRING" id="30522.A0A4W2BUJ2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009095.1">
        <property type="protein sequence ID" value="ENSBIXP00000003827.1"/>
        <property type="gene ID" value="ENSBIXG00000010218.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042105">
        <property type="term" value="C:alpha-beta T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045059">
        <property type="term" value="P:positive thymic T cell selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001422">
        <property type="entry name" value="T-cell surface glycoprotein CD3 epsilon chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015484">
        <property type="entry name" value="CD3_esu/gsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003110">
        <property type="entry name" value="Phos_immunorcpt_sig_ITAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570:SF9">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 GAMMA CHAIN / DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16681">
        <property type="entry name" value="Ig_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00077">
        <property type="entry name" value="ITAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51055">
        <property type="entry name" value="ITAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021313365" description="T-cell surface glycoprotein CD3 epsilon chain" evidence="17">
        <location>
            <begin position="22"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin subtype 2" evidence="18">
        <location>
            <begin position="39"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="197" mass="22096" checksum="41098928F0FE3DBC" modified="2019-09-18" version="1">MQSGNLWRALGLCLLLVGAWAQDSEEIDADEQKPYEVSISGNTVELTCPREFEGEIHWKQNDEQMKGYTGKQLLLENFSEMDNSGYYQCYMTEGNKEAAHTLYLKARVCQNCMEVNLMEVATIIVVDICVTLGLLLLVYYWSKSRKAKASPMTRGAGAGGRPRGQNKGRPPPVPNPDYEPIRKGQRDLYAGLNQRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BV02</accession>
    <name>A0A4W2BV02_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glutamine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">QARS1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamine--tRNA ligase. Plays a critical role in brain development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with RARS1. Part of a complex composed of RARS1, QARS1 and AIMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BV02"/>
    <dbReference type="SMR" id="A0A4W2BV02"/>
    <dbReference type="STRING" id="30522.A0A4W2BV02"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008741.1">
        <property type="protein sequence ID" value="ENSBIXP00000004004.1"/>
        <property type="gene ID" value="ENSBIXG00000007426.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023096.1">
        <property type="protein sequence ID" value="ENSBIXP00005036053.1"/>
        <property type="gene ID" value="ENSBIXG00005017449.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074972"/>
    <dbReference type="OMA" id="TWCIYPM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001234">
        <property type="term" value="P:negative regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032873">
        <property type="term" value="P:negative regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1290:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000008">
        <property type="entry name" value="probable glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000049">
        <property type="entry name" value="Probable glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000006">
        <property type="entry name" value="Putative glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
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        <property type="entry name" value="Gln-tRNA-synth"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal protein L25-like"/>
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            <begin position="7"/>
            <end position="162"/>
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        <location>
            <begin position="165"/>
            <end position="254"/>
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        <location>
            <begin position="263"/>
            <end position="562"/>
        </location>
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        <location>
            <begin position="565"/>
            <end position="665"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF03950"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF20974"/>
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            <dbReference type="Ensembl" id="ENSBIXP00000004004.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="775" mass="87643" checksum="C30C9EE302D532F0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BXE8</accession>
    <name>A0A4W2BXE8_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3">Small integral membrane protein 19</fullName>
        </recommendedName>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BXM0</accession>
    <name>A0A4W2BXM0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="gene ID" value="ENSBIXG00000010385.1"/>
    </dbReference>
    <dbReference type="OMA" id="IWIRNTE"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="679" mass="80428" checksum="9B811794A44155A7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C028</accession>
    <name>A0A4W2C028_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Non-POU domain-containing octamer-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">NONO</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="26">Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with CPNE4 (via VWFA domain). Forms heterodimers with PSPC1; this involves formation of a coiled coil domain by helices from both proteins. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 and SPIB. Interacts with RNF43. Interacts with PER1 and PER2. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts (via second RRM domain) with WASL; the interaction is direct. Component of a multiprotein complex with WASL and SFPQ. Interacts with ERCC6. Interacts (via DNA-binding domain) with TET1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027390539.1">
        <property type="nucleotide sequence ID" value="XM_027534738.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027390540.1">
        <property type="nucleotide sequence ID" value="XM_027534739.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027390541.1">
        <property type="nucleotide sequence ID" value="XM_027534740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C028"/>
    <dbReference type="SMR" id="A0A4W2C028"/>
    <dbReference type="STRING" id="30522.A0A4W2C028"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006594.1">
        <property type="protein sequence ID" value="ENSBIXP00000005028.1"/>
        <property type="gene ID" value="ENSBIXG00000006740.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887558"/>
    <dbReference type="CTD" id="4841"/>
    <dbReference type="OMA" id="HGMAMNR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12946">
        <property type="entry name" value="NOPS_p54nrb_PSF_PSPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12588">
        <property type="entry name" value="RRM1_p54nrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000226">
        <property type="entry name" value="Non-POU domain-containing octamer-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000043">
        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012975">
        <property type="entry name" value="NOPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034552">
        <property type="entry name" value="p54nrb_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08075">
        <property type="entry name" value="NOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="20" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="18 29" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="22" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="24" id="KW-0234">DNA repair</keyword>
    <keyword evidence="19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="23" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="15 28" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="21" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="31">
        <location>
            <begin position="73"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="31">
        <location>
            <begin position="147"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="442"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="29">
        <location>
            <begin position="274"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="30">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00063155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00068166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="470" mass="54121" checksum="CD1B68A1F49F5E83" modified="2019-09-18" version="1">MQSNKTFNLEKQNHTPRKHHQHHHQQHHQQQQQQPPPPPMPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3Y3</accession>
    <name>A0A4W2C3Y3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3Y3"/>
    <dbReference type="STRING" id="30522.A0A4W2C3Y3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004122.1">
        <property type="protein sequence ID" value="ENSBIXP00000006252.1"/>
        <property type="gene ID" value="ENSBIXG00000012375.1"/>
    </dbReference>
    <dbReference type="OMA" id="MDQVREC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000944">
        <property type="entry name" value="Testis-specific serine/threonine-protein kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF84">
        <property type="entry name" value="TESTIS SPECIFIC SERINE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="321"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="384" mass="43127" checksum="BF17E869273333E8" modified="2019-09-18" version="1">MKAAGRKKLDQRAFLEQVHECRDNGYLLSSKKIGSGAFSKVYLAYATQERMRHNSKLASDLRGKRHTMVAIKIVSTAEAPAEFSRKFLPREISSLNATYKHLNVVQLYETFANSRRTYLVLELAAHGDLLEHINATSAHRRCPGLEEEEARRLLWQLVSAVAHCHNSGIVHRDLKCENILLDDRGCLKLSDFGFANRSGLKNSLLSTFCGSVAYTAPEILMSKKYNGEQADLWSLGVILYAMVTGKLPFKECQPHGMLYLMRRGPTFRPGLSPECQDLIRGLLQLRPRARLALQQVATHHWMLPAAHALFCTTLCAIPGRGPQKRKTPGTHKELSFDLSSSRAGPSSGGSTRTEDKRAGGWWWGALQREAWKGRETGIHGRRWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7X8</accession>
    <name>A0A4W2C7X8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ASD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2C7X8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011990.1">
        <property type="protein sequence ID" value="ENSBIXP00000002397.1"/>
        <property type="gene ID" value="ENSBIXG00000008875.1"/>
    </dbReference>
    <dbReference type="OMA" id="LHCSDFD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014799">
        <property type="entry name" value="ASD2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF35">
        <property type="entry name" value="PROTEIN SHROOM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08687">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51307">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASD2" evidence="6">
        <location>
            <begin position="1108"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="744"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="907"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="989"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1042"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="744"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1067"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1078"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1414" mass="156237" checksum="4787EAD3F9AA2F89" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAX3</accession>
    <name>A0A4W2CAX3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ATP6V0D1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR. Interacts with ATP6AP2; ATP6AP2 is a V-ATPase accessory protein and the interaction promotes v-ATPase complex assembly. Interacts with TMEM9; TMEM9 is a v-ATPase assembly regulator and the interaction induces the interaction with ATP6AP2. Interacts with PIP4P1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Lysosome membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372067.1">
        <property type="nucleotide sequence ID" value="XM_027516266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAX3"/>
    <dbReference type="SMR" id="A0A4W2CAX3"/>
    <dbReference type="STRING" id="30522.A0A4W2CAX3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018574.1">
        <property type="protein sequence ID" value="ENSBIXP00000009989.1"/>
        <property type="gene ID" value="ENSBIXG00000006271.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030980.1">
        <property type="protein sequence ID" value="ENSBIXP00005018556.1"/>
        <property type="gene ID" value="ENSBIXG00005003697.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876725"/>
    <dbReference type="CTD" id="9114"/>
    <dbReference type="GeneTree" id="ENSGT00390000002200"/>
    <dbReference type="OMA" id="MTYGYMI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036295">
        <property type="term" value="P:cellular response to increased oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097401">
        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.50:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="351" mass="40329" checksum="A720F8A87511203C" modified="2019-09-18" version="1">MSFFPELYFNVDNGYLEGLVRGLKAGVLSQADYLNLVQCETLEDLKLHLQSTDYGNFLANEASPLTVSVIDDRLKEKMVVEFRHMRNHAYEPLASFLDFITYSYMIDNVILLITGTLHQRSIAELVPKCHPLGSFEQMEAVNIAQTPAELYNAILVDTPLAAFFQDCISEQDLDEMNIEIIRNTLYKAYLESFYKFCTLLGGTTADAMCPILEFEADRRAFIITINSFGTELSKEDRAKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEGAGSNPGDKTLEDRFFEHEVKLNKLAFLNQFHFGVFYAFVKLKEQECRNIVWIAECIAQRHRAKIDNYIPIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAY0</accession>
    <name>A0A4W2CAY0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Putative E3 ubiquitin-protein ligase UBR7</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">N-recognin-7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase UBR7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">UBR7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAY0"/>
    <dbReference type="STRING" id="30522.A0A4W2CAY0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043775.1">
        <property type="protein sequence ID" value="ENSBIXP00000008761.1"/>
        <property type="gene ID" value="ENSBIXG00000014528.1"/>
    </dbReference>
    <dbReference type="OMA" id="GAMVYNH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15542">
        <property type="entry name" value="PHD_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000183">
        <property type="entry name" value="putative E3 ubiquitin-protein ligase UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="17">
        <location>
            <begin position="44"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="16">
        <location>
            <begin position="44"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="425" mass="48222" checksum="8161FFB261C4EDC0" modified="2019-09-18" version="1">MAGAEEPAGRQSELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVKRQALYACSTCTPEGEEPAGICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLFPDKAKINSGNKYNDNFFGLYCICKRPYPDPEDEIPDEMIQCIVCEDWFHGRHLGAIPPESGDFQEMVCQACMKRCSFLWAYAAQLAVTKVTTEDDGLVLNVDGVGDQEVIKTENGAHQDNALEEDVPEHGKAIVKEVKAEQTNEPCTSSSSESDLQKAFQNQHLNTESQSGCKLEELKAKQFIKKDTATYWPLNWRSKLCTCKDCMKMYGDLDVLFLTDEYDTVLAYENKGKVDQAADRRDPLMDTLNSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRRRVDGMQYYCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CB62</accession>
    <name>A0A4W2CB62_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger AN1-type containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CB62"/>
    <dbReference type="SMR" id="A0A4W2CB62"/>
    <dbReference type="STRING" id="30522.A0A4W2CB62"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018798.1">
        <property type="protein sequence ID" value="ENSBIXP00000010131.1"/>
        <property type="gene ID" value="ENSBIXG00000015690.1"/>
    </dbReference>
    <dbReference type="OMA" id="RQYCLKH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF37">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="58"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="268" mass="30361" checksum="335A4184B96A4FAB" modified="2019-09-18" version="1">MAEMDVGRHCQVERCRRLDFLPFVCDGCSGVFCLEHRSRESHSCPEVTVINKRLKSDTGTSHPCSFRDCAERELVPIMCPSCEKNFCLRHRHQSDHECEKLEIPKPRMAATQKLVKDIIDSKTGETTSKRRKGAKNSETAAKVALMKLKMHAAGDKSLPQTERIYFQVFLPKGSKEKRKAMFFCRGWSVGKATDFAASLASLKNDNNRLTAKKLRLCHSVSGEALPLDHILETWIAKEDCPLHSGGNIILEYLSDEEQFLKNVDSYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CE52</accession>
    <name>A0A4W2CE52_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rab11 family-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RAB11FIP2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. Plays an essential role in phagocytosis through a mechanism involving TICAM2, RAC1 and CDC42 Rho GTPases for controlling actin-dynamics.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homooligomerizes in a Rab11-independent manner. Forms a heterooligomeric complex with RAB11FIP4. Interacts with AP2A1, MYO5B, RAB25 and REPS1. Interacts with RAB11A and RAB11B (activated GTP-bound form). Interacts with NPC1L1. Interacts (via NPF motifs) with EHD1 and EHD3. Interacts with TICAM2; this interaction directs RAB11FIP2 to the phagosome. Interacts with RAB14 and RAB25 (GTP-bound forms).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027385316.1">
        <property type="nucleotide sequence ID" value="XM_027529515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CE52"/>
    <dbReference type="SMR" id="A0A4W2CE52"/>
    <dbReference type="STRING" id="30522.A0A4W2CE52"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007408.1">
        <property type="protein sequence ID" value="ENSBIXP00000004634.1"/>
        <property type="gene ID" value="ENSBIXG00000000728.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027071.1">
        <property type="protein sequence ID" value="ENSBIXP00005037768.1"/>
        <property type="gene ID" value="ENSBIXG00005003218.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884682"/>
    <dbReference type="CTD" id="22841"/>
    <dbReference type="GeneTree" id="ENSGT00940000158482"/>
    <dbReference type="OMA" id="GETHQES"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001891">
        <property type="term" value="C:phagocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035773">
        <property type="term" value="P:insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045055">
        <property type="term" value="P:regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035669">
        <property type="term" value="P:TRAM-dependent toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08682">
        <property type="entry name" value="C2_Rab11-FIP_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2440:FF:000002">
        <property type="entry name" value="rab11 family-interacting protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000070">
        <property type="entry name" value="rab11 family-interacting protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037789">
        <property type="entry name" value="FIP_classI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15746:SF20">
        <property type="entry name" value="RAB11 FAMILY-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15746">
        <property type="entry name" value="RAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="FIP-RBD" evidence="15">
        <location>
            <begin position="436"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="263"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="511" mass="58049" checksum="F82A47AD9247DC27" modified="2019-09-18" version="1">MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLMQGNPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRAKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKTDGTFSDTSSAIIPSSHMPDAHTEFSSSEIQMKSKPKKPFLLGPQRLSSAHSMSDLTGAHVCSEKLKSGAVAQTHLLGRQIDSFGAVPESGSLKSPHRRTLSFDTSKMNQPDSSVDAGESAFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSESWDSSVNLFSKSIEVRKENKREKREKVSLFERVTGKKDRRSDKLNNGGADSPCDLKSPNAFSEHRQDYFDYESTNPFTTKFRASNIMPSSSFHVNPTSHEDLRKIPESNPFDATAGYRSLTYEEVLQELVKHKELLRRKDSHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFM6</accession>
    <name>A0A4W2CFM6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc fingers and homeoboxes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">C14H8orf76</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CFM6"/>
    <dbReference type="SMR" id="A0A4W2CFM6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021335.1">
        <property type="protein sequence ID" value="ENSBIXP00000011789.1"/>
        <property type="gene ID" value="ENSBIXG00000016962.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041404">
        <property type="entry name" value="DUF5588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31919">
        <property type="entry name" value="ZINC FINGERS AND HOMEOBOXES PROTEIN 1, ISOFORM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31919:SF1">
        <property type="entry name" value="ZINC FINGERS AND HOMEOBOXES PROTEIN 1, ISOFORM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17826">
        <property type="entry name" value="DUF5588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="43249" checksum="0514E0C6D7C50783" modified="2019-09-18" version="1">METGCWLLGGEFEDSVFEERRERRPGPPESYRAKRCEPQWFYEETGSSDDVEALTVQKFKGDLAYRRQEYQKALQEYSSISEKLPSTNFAMKRDVQEGQARCLVHLGRHKEALEIATNLENKATNTDHLTTVLYLQFTICSSLQNLEKTIFCLQKLISLHPFNPWNWGRLGEAYLNLGPVLSASLASSQKENSFTSSDTAIKSSFPHSGKDCLLCFPETLPEGSVEVSSGNKQRNEKALKNIQNRMTEERAAVLLETQMKACASFIRTRLLLQLAQCQQTSFALERNLKTQQEIEDKMKRFSFKEDTLLLIAEVMGEDIVPEKIRDEVHTEVKCVGPAALTALVIGSSKEFEDKWFRKIKDHFCPFENQFHTEIQILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGT3</accession>
    <name>A0A4W2CGT3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AFG1-like ATPase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Lactation elevated protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain. Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Found in several complexes of 140-500 kDa. Interacts with YME1L1. Interacts with COX4I1. Interacts with COX5A. Interacts with TP53; mediates mitochondrial translocation of TP53 in response to genotoxic stress such as mitomycin C treatment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGT3"/>
    <dbReference type="STRING" id="30522.A0A4W2CGT3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020301.1">
        <property type="protein sequence ID" value="ENSBIXP00000011115.1"/>
        <property type="gene ID" value="ENSBIXG00000016459.1"/>
    </dbReference>
    <dbReference type="OMA" id="ARRFINM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000735">
        <property type="entry name" value="Lactation elevated 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="551" mass="62372" checksum="3D82BEBF63C74FBB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIN2</accession>
    <name>A0A4W2CIN2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LMTK3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="STRING" id="30522.A0A4W2CIN2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023087.1">
        <property type="protein sequence ID" value="ENSBIXP00000012915.1"/>
        <property type="gene ID" value="ENSBIXG00000017927.1"/>
    </dbReference>
    <dbReference type="OMA" id="EKKPESW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000347">
        <property type="entry name" value="Apoptosis associated tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000275">
        <property type="entry name" value="Apoptosis associated tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE LMTK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LMTK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="178"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="458"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="531"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="589"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="731"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="915"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="792"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="809"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="866"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="980"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1108"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1135"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1151"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1194"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1233"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1266"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1297"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1311"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1327"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1419"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1495" mass="157085" checksum="7381C0D288B3831E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ58</accession>
    <name>A0A4W2CJ58_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kelch-like protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KLHL20</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the BCR(KLHL20) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL20 and RBX1. Interacts with PDZ-RhoGEF/ARHGEF11, DAPK1, PML and CORO7. Interacts with F-actin. Interacts with IFN-gamma (IFNG). Interacts (via kelch repeats) with IVNS1ABP (via kelch repeats); this interaction blocks the assembly of CUL3-KLHL20 complex.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027421154.1">
        <property type="nucleotide sequence ID" value="XM_027565353.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027421155.1">
        <property type="nucleotide sequence ID" value="XM_027565354.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027421156.1">
        <property type="nucleotide sequence ID" value="XM_027565355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJ58"/>
    <dbReference type="SMR" id="A0A4W2CJ58"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023401.1">
        <property type="protein sequence ID" value="ENSBIXP00000013138.1"/>
        <property type="gene ID" value="ENSBIXG00000018101.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012343.1">
        <property type="protein sequence ID" value="ENSBIXP00005002388.1"/>
        <property type="gene ID" value="ENSBIXG00005008757.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906759"/>
    <dbReference type="CTD" id="27252"/>
    <dbReference type="GeneTree" id="ENSGT00940000155161"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019964">
        <property type="term" value="F:type II interferon binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990390">
        <property type="term" value="P:protein K33-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18459">
        <property type="entry name" value="BACK_KLHL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18249">
        <property type="entry name" value="BTB_POZ_KLHL20_KLEIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000006">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF451">
        <property type="entry name" value="KELCH-LIKE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="68"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="609" mass="67955" checksum="FA05E3FE8341B422" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJU3</accession>
    <name>A0A4W2CJU3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CJU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023720.1">
        <property type="protein sequence ID" value="ENSBIXP00000013345.1"/>
        <property type="gene ID" value="ENSBIXG00000018281.1"/>
    </dbReference>
    <dbReference type="OMA" id="YGHRRYD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002668">
        <property type="entry name" value="Paternally expressed 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001757">
        <property type="entry name" value="paternally-expressed gene 3 protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002097">
        <property type="entry name" value="paternally-expressed gene 3 protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000661">
        <property type="entry name" value="paternally-expressed gene 3 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF357">
        <property type="entry name" value="PATERNALLY-EXPRESSED GENE 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="384"/>
            <end position="411"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="402" mass="43698" checksum="79D43747C93F4157" modified="2019-09-18" version="1">MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPASGSSSPLRGESAADSDGWDSAPSDLRTIQTFVKKAKSSKRRAAQAGPTQPGPPRSTFPRLQAPDSATLLEKMKLKDSLFDIDGPKMASPLSPTSLTHASRPPAALTPVPLSQGDLSQPPRKKDRKNRKLGPGGATGFGVLRRPRPAPGDGEKRSRIKKSKKRKLKKAERGDRLPPPGPPRAPPSDTDSEEEEEEEEEEEEEEMAAMVGGEAPAPVLPTPEAPRPPATVHPEGAPPTDGESKEVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRPMIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGPPKSGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CT20</accession>
    <name>A0A4W2CT20_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Anaphase-promoting complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Cyclosome subunit 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ANAPC7</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. APC7 is not required for the assembly of the APC/C complex, but has an enzyme-substrate adapter activity mediating the processive ubiquitination of specific substrates. Involved in brain development through the specific ubiquitination and clearance of MKI67 from constitutive heterochromatin after neuronal progenitors exit mitosis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">V-shaped homodimer. The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the APC7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027422615.1">
        <property type="nucleotide sequence ID" value="XM_027566814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CT20"/>
    <dbReference type="SMR" id="A0A4W2CT20"/>
    <dbReference type="STRING" id="30522.A0A4W2CT20"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017706.1">
        <property type="protein sequence ID" value="ENSBIXP00000009430.1"/>
        <property type="gene ID" value="ENSBIXG00000006578.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029552.1">
        <property type="protein sequence ID" value="ENSBIXP00005017606.1"/>
        <property type="gene ID" value="ENSBIXG00005021108.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907532"/>
    <dbReference type="CTD" id="51434"/>
    <dbReference type="GeneTree" id="ENSGT00950000182950"/>
    <dbReference type="OMA" id="MGECYYY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141198">
        <property type="term" value="P:protein branched polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000068">
        <property type="entry name" value="Anaphase promoting complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000126">
        <property type="entry name" value="Anaphase promoting complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000178">
        <property type="entry name" value="Anaphase promoting complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000238">
        <property type="entry name" value="Anaphase promoting complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF36">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 19" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="372"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="508"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="546"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="598" mass="66618" checksum="7288CC897C14C6C2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CU26</accession>
    <name>A0A4W2CU26_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Defensin beta 129</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DEFB129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CU26"/>
    <dbReference type="STRING" id="30522.A0A4W2CU26"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028975.1">
        <property type="protein sequence ID" value="ENSBIXP00000016840.1"/>
        <property type="gene ID" value="ENSBIXG00000020916.1"/>
    </dbReference>
    <dbReference type="OMA" id="RRCLMGF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021207654" evidence="2">
        <location>
            <begin position="20"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="179" mass="20241" checksum="B64F44A3E164EE1E" modified="2019-09-18" version="1">MKLLFPIFASLMLQWQVNTEYFGLRRCLMGLGRCKEHCNMDEKELDKCKKKTCCIRSKVVQLIKNYIQNEMLHMLKEDSQEVLKITKNFSVMMQTEHHNLSVLPKIKSANAFAKINTIIIPNATIVNSATTNPVNSGKIIHTATSTRKRRDLGTDSPPPAPPPSYILPTALELEADEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUP5</accession>
    <name>A0A4W2CUP5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUP5"/>
    <dbReference type="STRING" id="30522.A0A4W2CUP5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018616.1">
        <property type="protein sequence ID" value="ENSBIXP00000010008.1"/>
        <property type="gene ID" value="ENSBIXG00000002322.1"/>
    </dbReference>
    <dbReference type="OMA" id="AYNIQEE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
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            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
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            <end position="228"/>
        </location>
    </feature>
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        <location>
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            <end position="304"/>
        </location>
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            <end position="380"/>
        </location>
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    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
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        <location>
            <begin position="457"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
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            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
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            <end position="684"/>
        </location>
    </feature>
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        <location>
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            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
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            <end position="836"/>
        </location>
    </feature>
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        <location>
            <begin position="837"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="913"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="989"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="1065"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1146" mass="128851" checksum="400F858D260E2A05" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CV73</accession>
    <name>A0A4W2CV73_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CV73"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027457.1">
        <property type="protein sequence ID" value="ENSBIXP00000015829.1"/>
        <property type="gene ID" value="ENSBIXG00000020201.1"/>
    </dbReference>
    <dbReference type="OMA" id="CITFSWV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF78">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 7A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="52"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="334" mass="37469" checksum="01EF2C844BCB4914" modified="2019-09-18" version="1">GNTGLIPGGRIKIPHLCYEHLNGSCVKTPYSPGPRLILYTVFIFGAVRTVFGNLLVMISILHFKQLHSPANFLITSLACADFLVGVTVTPFSTVRSMESCWYSGESYCKFHTCFEGSFCYASIYHLCFISLDRYIAVTDPLVYPTKFTVSVSGMCIVFSWLFPIIYTFSLLGTGVNAAGLEDLVSALTCVGGCQIAVNQSWVLVNFLLFFIPTLAMIVLYSKISSFNKPGRCSESYQDRVAKRERKAARTLGMAVLAFLVSWLPYFLDSIIDAFLGFITPTYVYEILVWIAYYNSAMNPLIYAFFYPWFRKAIKLIVTGKVLRENSSTIHLFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXJ9</accession>
    <name>A0A4W2CXJ9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(27) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.356</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EZH1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.356" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027372738.1">
        <property type="nucleotide sequence ID" value="XM_027516937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXJ9"/>
    <dbReference type="SMR" id="A0A4W2CXJ9"/>
    <dbReference type="STRING" id="30522.A0A4W2CXJ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020199.1">
        <property type="protein sequence ID" value="ENSBIXP00000011059.1"/>
        <property type="gene ID" value="ENSBIXG00000002531.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877125"/>
    <dbReference type="CTD" id="2145"/>
    <dbReference type="OMA" id="HMVGASC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19217">
        <property type="entry name" value="SET_EZH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000001">
        <property type="entry name" value="Putative histone-lysine N-methyltransferase EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048358">
        <property type="entry name" value="EZH1/2_MCSS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021654">
        <property type="entry name" value="EZH1/EZH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044438">
        <property type="entry name" value="EZH1_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041343">
        <property type="entry name" value="PRC2_HTH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EZH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21358">
        <property type="entry name" value="Ezh2_MCSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11616">
        <property type="entry name" value="EZH2_WD-Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18118">
        <property type="entry name" value="PRC2_HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="14">
        <location>
            <begin position="504"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="13">
        <location>
            <begin position="613"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="186"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="375"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="747" mass="85285" checksum="A40D3D08F63CA155" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0N9</accession>
    <name>A0A4W2D0N9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DLG associated protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DLGAP4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPAP family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027558625.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2D0N9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030416.1">
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        <property type="gene ID" value="ENSBIXG00000021695.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006561.1">
        <property type="protein sequence ID" value="ENSBIXP00005005337.1"/>
        <property type="gene ID" value="ENSBIXG00005011119.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903067"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155308"/>
    <dbReference type="OMA" id="QIFSHEE"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:postsynaptic specialization"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090128">
        <property type="term" value="P:regulation of synapse maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005026">
        <property type="entry name" value="SAPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353:SF19">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN DAP SAP90/PSD-95-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03359">
        <property type="entry name" value="GKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="664"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="763"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="915"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="528"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="600"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="915"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="940"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="969"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="992" mass="107842" checksum="E2B2B164FA5B6DA7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D132</accession>
    <name>A0A4W2D132_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Nuclear receptor coactivator 6-interacting protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PRIP-interacting protein with methyltransferase motif</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D132"/>
    <dbReference type="STRING" id="30522.A0A4W2D132"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045541.1">
        <property type="protein sequence ID" value="ENSBIXP00000012347.1"/>
        <property type="gene ID" value="ENSBIXG00000017440.1"/>
    </dbReference>
    <dbReference type="OMA" id="QSEPHNG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000066">
        <property type="entry name" value="Trimethylguanosine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="111"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="266"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="350"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="525"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00064494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="853" mass="95981" checksum="5E5A3D4500E2E14C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1I9</accession>
    <name>A0A4W2D1I9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1I9"/>
    <dbReference type="STRING" id="30522.A0A4W2D1I9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033414.1">
        <property type="protein sequence ID" value="ENSBIXP00000019698.1"/>
        <property type="gene ID" value="ENSBIXG00000023241.1"/>
    </dbReference>
    <dbReference type="OMA" id="WLFEMNV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L54, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="163" mass="18485" checksum="3FC8F6135DF743E3" modified="2019-09-18" version="1">MAARRLFGATGSWARWRAWELPDPAGSVRLHVRDYAKRPVFKGGKGAKGAAVGETLKDPEVCTDPVQLTTHAMGVNIYKEGQDVVLKPDSEYPEWLFQMNVGPPKKLEELDPETREYWRLLRKHNIWRHNRLSKNQKFYLPTILASLPTILSTPTGAHQLRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D344</accession>
    <name>A0A4W2D344_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFU1 iron-sulfur cluster scaffold homolog, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HIRA-interacting protein 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Iron-sulfur cluster scaffold protein which can assemble [4Fe-4S] clusters and deliver them to target proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer and homohexamer; the apo-NFU1 is a monomer, while the holo-NFU1 is a hexamer composed of a trimer of dimer that is probably linked by some 4Fe-4S cluster. Interacts with HIRA and EPM2A/laforin. Interacts with BOLA3. Interacts with HSPA9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D344"/>
    <dbReference type="STRING" id="30522.A0A4W2D344"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031774.1">
        <property type="protein sequence ID" value="ENSBIXP00000018629.1"/>
        <property type="gene ID" value="ENSBIXG00000004351.1"/>
    </dbReference>
    <dbReference type="OMA" id="AIMEHYM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.70:FF:000002">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="12">
        <location>
            <begin position="61"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="254" mass="28217" checksum="26724B2BD81F366B" modified="2019-09-18" version="1">MAVAAARLGWGVAAAAAGLRSRFCHVTSPYTIKKQLLHQFFQRPLFPLPATLCNTVRYMFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPATAFRSPLARQLFRIEGVKSVFFGPDFITVTKENEELDWNLLKPDIYATIMDFFASGLPLVTEETPSGEAGSEDDDEVVAMIKELLDTRIRPTVQEDGGDVIYKGFEDGIVQLKLQGSCTSCPSSIITLKNGIQNMLQFYIPEVEGVEQVMDDESDEKEANSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3N2</accession>
    <name>A0A4W2D3N2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Toll-like receptor 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">TLR4</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027406451.1">
        <property type="nucleotide sequence ID" value="XM_027550650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3N2"/>
    <dbReference type="SMR" id="A0A4W2D3N2"/>
    <dbReference type="STRING" id="30522.A0A4W2D3N2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032096.1">
        <property type="protein sequence ID" value="ENSBIXP00000018826.1"/>
        <property type="gene ID" value="ENSBIXG00000022593.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011416.1">
        <property type="protein sequence ID" value="ENSBIXP00005002851.1"/>
        <property type="gene ID" value="ENSBIXG00005009147.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897722"/>
    <dbReference type="CTD" id="7099"/>
    <dbReference type="GeneTree" id="ENSGT00940000160778"/>
    <dbReference type="OMA" id="CKHSAER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046696">
        <property type="term" value="C:lipopolysaccharide receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001891">
        <property type="term" value="C:phagocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001875">
        <property type="term" value="F:lipopolysaccharide immune receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014002">
        <property type="term" value="P:astrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002322">
        <property type="term" value="P:B cell proliferation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904646">
        <property type="term" value="P:cellular response to amyloid-beta"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071223">
        <property type="term" value="P:cellular response to lipoteichoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140052">
        <property type="term" value="P:cellular response to oxidised low-density lipoprotein particle stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036120">
        <property type="term" value="P:cellular response to platelet-derived growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071346">
        <property type="term" value="P:cellular response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032497">
        <property type="term" value="P:detection of lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070371">
        <property type="term" value="P:ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    </evidence>
    <sequence length="841" mass="96015" checksum="603936A17E834735" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7B3</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="46"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="322" mass="36386" checksum="28BB0484D7D2A569" modified="2019-09-18" version="1">MYSENQTSQNQTLNTDFILVGLFGETKHAFLLYTATLIFFLMALAGNALLIILVHMEPRLHTPMYFFISQLSLMDLMYISVTVPKMLLGQVTGDHTISPSGCGIQMFFYLTLAGAEFFLLSAMAYDRYAAMCRPLHYALLMNQRVCECLVSGCWFLGMVDGLLLTPIAMSFPFCHSRKILSFFCEAPALMKLSCSDISLYKMVMYLCCVLMLLIPTVVISSSYALILHLIHRMSSSESRRKAFATCSSHMIVVLLFFGAAIYTYMLPSFYHTAKQDMMVSAFYTIITPVLNTLIYSLRNKDVTGALRSKMQSGEPRNKTFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7J4</accession>
    <name>A0A4W2D7J4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CDC42 small effector protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CDC42SE1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with CDC42 (in GTP-bound form). Interacts weakly with RAC1 and not at all with RHOA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC42SE/SPEC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027390034.1">
        <property type="nucleotide sequence ID" value="XM_027534233.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027390040.1">
        <property type="nucleotide sequence ID" value="XM_027534239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7J4"/>
    <dbReference type="STRING" id="30522.A0A4W2D7J4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036810.1">
        <property type="protein sequence ID" value="ENSBIXP00000021918.1"/>
        <property type="gene ID" value="ENSBIXG00000024912.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040727.1">
        <property type="protein sequence ID" value="ENSBIXP00005043906.1"/>
        <property type="gene ID" value="ENSBIXG00005027674.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887217"/>
    <dbReference type="CTD" id="56882"/>
    <dbReference type="GeneTree" id="ENSGT00940000160112"/>
    <dbReference type="OMA" id="DRPWNNS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000015">
        <property type="entry name" value="CDC42 small effector protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039056">
        <property type="entry name" value="SPEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502:SF3">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="16">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8925" checksum="D1C1DCEBD339EF5B" modified="2019-09-18" version="1">MSEFWHKLGCCVVEKPQPKKKRRRIDRTMIGEPMNFVHLTHIGSGEMGAGDGLAMTGAVQEQMRSKGNRDRPWSNSRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D865</accession>
    <name>A0A4W2D865_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Early endosome antigen 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000034549.1">
        <property type="protein sequence ID" value="ENSBIXP00000020465.1"/>
        <property type="gene ID" value="ENSBIXG00000023775.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15730">
        <property type="entry name" value="FYVE_EEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.390:FF:000006">
        <property type="entry name" value="Early endosome antigen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000180">
        <property type="entry name" value="Early endosome antigen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.390">
        <property type="entry name" value="L1 transposable element, trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164:SF30">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="1356"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="477"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="129"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="240"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="891"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1176"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="7">
        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1415" mass="162686" checksum="AA7EB9BDC95083AA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8U7</accession>
    <name>A0A4W2D8U7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ADAM metallopeptidase domain 33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ADAM33</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027415153.1">
        <property type="nucleotide sequence ID" value="XM_027559352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8U7"/>
    <dbReference type="STRING" id="30522.A0A4W2D8U7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026494.1">
        <property type="protein sequence ID" value="ENSBIXP00000015181.1"/>
        <property type="gene ID" value="ENSBIXG00000019740.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
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    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
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    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Disintegrin_dom_sf"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Peptidase_M12B_N"/>
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        <property type="entry name" value="Reprolysin_adamalysin"/>
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    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF38">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 33"/>
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    </dbReference>
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        <property type="entry name" value="Pep_M12B_propep"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
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    </dbReference>
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        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021500431" evidence="10">
        <location>
            <begin position="29"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="13">
        <location>
            <begin position="211"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="12">
        <location>
            <begin position="418"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="650"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="770"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="790" mass="85618" checksum="8794FA0C14B8B4CB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEX6</accession>
    <name>A0A4W2DEX6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Microtubule associated protein 1 light chain 3 alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MAP1LC3A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414554.1">
        <property type="nucleotide sequence ID" value="XM_027558753.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414556.1">
        <property type="nucleotide sequence ID" value="XM_027558755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEX6"/>
    <dbReference type="SMR" id="A0A4W2DEX6"/>
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        <property type="protein sequence ID" value="ENSBIXP00000017356.1"/>
        <property type="gene ID" value="ENSBIXG00000021357.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053593.1">
        <property type="protein sequence ID" value="ENSBIXP00005044818.1"/>
        <property type="gene ID" value="ENSBIXG00005002367.1"/>
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    <dbReference type="GeneID" id="113903099"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158853"/>
    <dbReference type="OMA" id="VNERSMV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044754">
        <property type="term" value="C:autolysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17234">
        <property type="entry name" value="Ubl_ATG8_MAP1LC3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000059">
        <property type="entry name" value="Microtubule-associated proteins 1A/1B light chain 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="12">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="121" mass="14286" checksum="496B4A43ED388043" modified="2019-09-18" version="1">MPSDRPFKQRRSFADRCKEVQQIREQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIU0</accession>
    <name>A0A4W2DIU0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Erythropoietin receptor</fullName>
            <shortName evidence="18">EPO-R</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">EPOR</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Forms homodimers on EPO stimulation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins including LYN, the adapter protein SH2B2, PTPN6, PTPN11, JAK2, PI3 kinases, STAT5A/B, SOCS3, CRKL. Interacts with INPP5D/SHIP1. SH2B2 binding inhibits the JAK-STAT signaling. Interacts with RHEX; this interaction occurs in a erythropoietin (EPO)-dependent manner. Interacts with ATXN2L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the type I cytokine receptor family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIU0"/>
    <dbReference type="SMR" id="A0A4W2DIU0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052798.1">
        <property type="protein sequence ID" value="ENSBIXP00000026236.1"/>
        <property type="gene ID" value="ENSBIXG00000028354.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004900">
        <property type="term" value="F:erythropoietin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001041">
        <property type="entry name" value="Erythropoietin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009167">
        <property type="entry name" value="Erythropoietin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015152">
        <property type="entry name" value="Growth/epo_recpt_lig-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003528">
        <property type="entry name" value="Long_hematopoietin_rcpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF28">
        <property type="entry name" value="ERYTHROPOIETIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09067">
        <property type="entry name" value="EpoR_lig-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001959">
        <property type="entry name" value="EPO_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01352">
        <property type="entry name" value="HEMATOPO_REC_L_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 23" id="KW-0732">Signal</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021418936" description="Erythropoietin receptor" evidence="23">
        <location>
            <begin position="26"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="147"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="466"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="site" description="Required for ligand binding" evidence="19">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with APS and STAT5, and" evidence="19">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="site" description="Required for STAT5/PTPN11/SOCS3 binding" evidence="19">
        <location>
            <position position="426"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with PTPN6" evidence="19">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <feature type="site" description="Required for STAT1/STAT3 activation" evidence="19">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="site" description="Required for CrkL binding" evidence="19">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001959-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="508" mass="55068" checksum="2592459D0F401DE3" modified="2019-09-18" version="1">MYHLPAALRAGIGFLCLLLAGAAWAPPPNPTDPKFENKAALLTAREPDEFLCFTERLEDLVCFWEEAATAGVGPDNYSFSYQLEGEPWKPCRLHQAPTARGLVRFWCSLPTADTSSFVPLELHVTAASGASRYRRTIHVNEVVLLDPPARLVARRADEGGHVVLRWLPPPGAPMASLIRYEVNISAENAAGSAQRVEILDGRTECLLSNLRGGTRYTFMVRARMAEPSFGGFWSAWSEPASLLTASDLDPLILTLSLVLVLILLLLAVLALLSHRRTLKQKIWPGIPSPESEFEGLFTTHKGNFQLWLYQTDGCLWWSPSTPLPEDPPAPLEVLSECCWGVTQAVEPGADDGGPLLEPVGSEHAQDSYLVLDKWLLPRSPPSEDLLQPGGDMDIVTMDEASEASSCTSALALKPGPEGASATSFEYTILDPSSQLLRPRALPPELLPTPPHLKYLYLVVSDSGISTDYSSGGSQEAQGGSSNGPYSNPYENSLIPAPKPSPPSYVACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJ63</accession>
    <name>A0A4W2DJ63_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">G protein-coupled receptor 108</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GPR108</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJ63"/>
    <dbReference type="STRING" id="30522.A0A4W2DJ63"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032225.1">
        <property type="protein sequence ID" value="ENSBIXP00000018914.1"/>
        <property type="gene ID" value="ENSBIXG00000002787.1"/>
    </dbReference>
    <dbReference type="OMA" id="RRGLGCW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF11">
        <property type="entry name" value="PROTEIN GPR108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021494070" evidence="8">
        <location>
            <begin position="33"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="9">
        <location>
            <begin position="264"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="548" mass="61403" checksum="C6901F53D3EAED3D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKE7</accession>
    <name>A0A4W2DKE7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 300</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium- and flagellum-specific protein that plays a role in axonemal structure organization and motility. May play a role in outer and inner dynein arm assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CFAP300 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKE7"/>
    <dbReference type="STRING" id="30522.A0A4W2DKE7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041126.1">
        <property type="protein sequence ID" value="ENSBIXP00000024781.1"/>
        <property type="gene ID" value="ENSBIXG00000006152.1"/>
    </dbReference>
    <dbReference type="OMA" id="DFVMACF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029416">
        <property type="entry name" value="CFAP300"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14926">
        <property type="entry name" value="CFAP300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="127" mass="14586" checksum="D36B8C3308AA6791" modified="2019-09-18" version="1">MAAGEPGDLGVYSFRFLPQKTFQSLSTPQTTSRLRQWSMLGRIEAQAFGFDQMFQAYRKDDFVMACFKDPNVIPNLKLLSESSGEWLTLGTESEYLFCVCEFTYSKEPRSHVICFHEMLQDAFILGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM42</accession>
    <name>A0A4W2DM42_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane serine protease 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMPRSS9</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DM42"/>
    <dbReference type="STRING" id="30522.A0A4W2DM42"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033822.1">
        <property type="protein sequence ID" value="ENSBIXP00000019954.1"/>
        <property type="gene ID" value="ENSBIXG00000004628.1"/>
    </dbReference>
    <dbReference type="OMA" id="HCFNHTR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF26">
        <property type="entry name" value="TRANSMEMBRANE SERINE PROTEASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="237"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="538"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="863"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="809"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1094" mass="117292" checksum="52C29F7C26738B4A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM66</accession>
    <name>A0A4W2DM66_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ethanolamine-phosphate phospho-lyase</fullName>
            <ecNumber evidence="9">4.2.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alanine--glyoxylate aminotransferase 2-like 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the pyridoxal-phosphate-dependent breakdown of phosphoethanolamine, converting it to ammonia, inorganic phosphate and acetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>phosphoethanolamine + H2O = acetaldehyde + NH4(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17889"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58190"/>
            <dbReference type="EC" id="4.2.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DM66"/>
    <dbReference type="STRING" id="30522.A0A4W2DM66"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042115.1">
        <property type="protein sequence ID" value="ENSBIXP00000025446.1"/>
        <property type="gene ID" value="ENSBIXG00000027529.1"/>
    </dbReference>
    <dbReference type="OMA" id="GAIETMK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050459">
        <property type="term" value="F:ethanolamine-phosphate phospho-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000058">
        <property type="entry name" value="ethanolamine-phosphate phospho-lyase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF1">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE PHOSPHO-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="440"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="525" mass="58404" checksum="0DFACFE96575C923" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP28</accession>
    <name>A0A4W2DP28_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">GTP-binding nuclear protein Ran</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DP28"/>
    <dbReference type="STRING" id="30522.A0A4W2DP28"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052725.1">
        <property type="protein sequence ID" value="ENSBIXP00000026174.1"/>
        <property type="gene ID" value="ENSBIXG00000028262.1"/>
    </dbReference>
    <dbReference type="OMA" id="FFWLARK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAN"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="216" mass="24437" checksum="D5C9A5B751A4A3E0" modified="2019-09-18" version="1">MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDITSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTQ5</accession>
    <name>A0A4W2DTQ5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Growth arrest specific 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GAS2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GAS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTQ5"/>
    <dbReference type="SMR" id="A0A4W2DTQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012923.1">
        <property type="protein sequence ID" value="ENSBIXP00000027585.1"/>
        <property type="gene ID" value="ENSBIXG00000000103.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21267">
        <property type="entry name" value="CH_GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000052">
        <property type="entry name" value="Growth arrest specific 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.920.20:FF:000003">
        <property type="entry name" value="Growth arrest-specific 2 like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46756:SF9">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46756">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="34"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="8">
        <location>
            <begin position="197"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="313" mass="34859" checksum="2BAC52CF52B12726" modified="2019-09-18" version="1">MCTALSPKVRSGPGLSDMHQYSQWLASRHEANLLPMKEDLALWLTNLLGKEITAETFMEKLDNGALLCQLAETVQEKFKESMDTTKPTKNLPLKKIPCKASAPSGSFFARDNTANFLSWCRDLGVDETCLFESEGLVLHKQPREVCLCLLELGRIAARYGVEPPGLIKLEKEIEQEETLSAPSPSPSPSSKSSGKKSTGNLLDDAVKRISEDPPCKCPSKFCVERLSQGRYRVGEKILFIRMLHNKHVMVRVGGGWETFAGYLLKHDPCRMLQISRVDGKTSPVQSKSPTLKDMNPDNYLVVSASYKAKKEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DVM5</accession>
    <name>A0A4W2DVM5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipase A2</fullName>
            <ecNumber evidence="23">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45472"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72829"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45473"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:41232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40431"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73009"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40432"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73008"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40816"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40911"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoglycerol + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44524"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84472"/>
            <dbReference type="ChEBI" id="CHEBI:84475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:44525"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:78097"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:45425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + H2O = a 1-acyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:64381"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:44605"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="20">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 23">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DVM5"/>
    <dbReference type="STRING" id="30522.A0A4W2DVM5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038671.1">
        <property type="protein sequence ID" value="ENSBIXP00000023187.1"/>
        <property type="gene ID" value="ENSBIXG00000025738.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTITCED"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000001">
        <property type="entry name" value="Basic phospholipase A2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF9">
        <property type="entry name" value="PHOSPHOLIPASE A2, MEMBRANE ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="20 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 23" id="KW-0964">Secreted</keyword>
    <keyword evidence="23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021447161" description="Phospholipase A2" evidence="23">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="24">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="46"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="69"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="144" mass="16404" checksum="E2C9DA44BBAC2642" modified="2019-09-18" version="1">MKTLLLLAVIMAIGLLQVHGGLLNFRKMIKFTTGKESVTSYRRYGCYCGTRGRGIPKDATDWCCRAHDCCYKNLESRGCRTKFLKYNVTYQEDQIVCEDADDCKSQVCQCDKIAANCFAANLKTYNKMLRFYNKFRCRGAAPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXR1</accession>
    <name>A0A4W2DXR1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXR1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009259.1">
        <property type="protein sequence ID" value="ENSBIXP00000028778.1"/>
        <property type="gene ID" value="ENSBIXG00000010136.1"/>
    </dbReference>
    <dbReference type="OMA" id="QWAEYKL"/>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="123"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="498" mass="56838" checksum="BB5374FC95D0906F" modified="2019-09-18" version="1">MPPGGVGPVLIRVWQWAEYKLGEFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDTDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDCKRSVFIPIPKKGNAKECSSYCTIALISHASKVMLKILQARLLQYVNRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYSCFIDYAKAFDCVDHNKLWEILKEMGIPDHVICLLRNLYAGQEATVRTGHRTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGININNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFGGSKVTADCDCSHEIKRCLLLGRKVMTNLDSIFQSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKISPGISLEGMMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZR7</accession>
    <name>A0A4W2DZR7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox B3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HOXB3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372879.1">
        <property type="nucleotide sequence ID" value="XM_027517078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZR7"/>
    <dbReference type="SMR" id="A0A4W2DZR7"/>
    <dbReference type="STRING" id="30522.A0A4W2DZR7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005327.1">
        <property type="protein sequence ID" value="ENSBIXP00000030016.1"/>
        <property type="gene ID" value="ENSBIXG00000011808.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015045.1">
        <property type="protein sequence ID" value="ENSBIXP00005001009.1"/>
        <property type="gene ID" value="ENSBIXG00005029591.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877194"/>
    <dbReference type="CTD" id="3213"/>
    <dbReference type="GeneTree" id="ENSGT00940000159774"/>
    <dbReference type="OMA" id="GCAAPQK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060324">
        <property type="term" value="P:face development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021615">
        <property type="term" value="P:glossopharyngeal nerve morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021546">
        <property type="term" value="P:rhombomere development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030878">
        <property type="term" value="P:thyroid gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025281">
        <property type="entry name" value="DUF4074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF11">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13293">
        <property type="entry name" value="DUF4074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="181"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="183"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="61"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="136"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="241"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="376"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="426" mass="44115" checksum="905F2B2969DDC389" modified="2019-09-18" version="1">MQKATYYDNTAAALFGGYPSYPGSNGFGYDGPPQPPFQAATHLESDYQRSACSLQSLGNAAPHAKGKELNGSCMRPGLASEPLPAPPGSPPPSAAPTSTTSNSSNGGGPSKSGPPKCGAGSNSTLTKQIFPWMKESRQTSKLKNSSPGTAEGCGSGGGGGGGSGSSGGGGGGGDKSPPGSAASKRARTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLSERQIKIWFQNRRMKYKKDQKAKGLASSSGGPSPAGSPSQPMQSTAGFMNALHSMTPSYESPSPPAFGKAHQNAYALPSNYQPPLKGCGAPQKYPQTPAPDYEPHVLQTNGGAYGTPTMQGSPVYVGGGGYADPLPPPAGPSLYGLNHLSHHPSGNLDYNGAPPMAPSQHHGPCDPHPTYTDLSSHHAPPPQGRIQEAPKLTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2U8</accession>
    <name>A0A4W2E2U8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E2U8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020055.1">
        <property type="protein sequence ID" value="ENSBIXP00000033536.1"/>
        <property type="gene ID" value="ENSBIXG00000016343.1"/>
    </dbReference>
    <dbReference type="OMA" id="VWITIKL"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="392" mass="44818" checksum="B603869F5DC1CF4D" modified="2019-09-18" version="1">MFKLILQKAEEPEIKLPTSAGSWKKQESSRKTSISALLTLPKSLTVWITIKLWKILKEMGIPDHLTCFLKNLYAGQEATVRTGHGTTDWFQIGKGVRQGSLSPCLFNLYTEYIMRNAGLEEAQAGIKTAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPIISWEIDGETVETVSDFIFLGFKITADTDCSHEIKRRLLLGRKVMTNLDSILKSRDITLPTKVCLVKAIFPVVMYGCESWTVKKAERRRIDAFELWYWRRLLRVPWTARRSNQSILKELSPGCSLEGLMLKLKLQYFGHVMRRVDSLEKTLMLGGIGGRRRRGRQRMRWLDGINDSMDMSLSELRELVMDREAWRAAIHGVAKSWTQLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5I5</accession>
    <name>A0A4W2E5I5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CENPB DNA-binding domain containing 1, pseudo</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CENPBD1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tigger transposable element derived protein family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027513718.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2E5I5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016479.1">
        <property type="protein sequence ID" value="ENSBIXP00000032009.1"/>
        <property type="gene ID" value="ENSBIXG00000014469.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005002897.1">
        <property type="protein sequence ID" value="ENSBIXP00005007186.1"/>
        <property type="gene ID" value="ENSBIXG00005001348.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000163154"/>
    <dbReference type="OMA" id="PCHPVNL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
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        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="HTH CENPB-TYPE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="TRANSPOSON"/>
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    </dbReference>
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        <property type="entry name" value="CENP-B_N"/>
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    </dbReference>
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        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="HTH_PSQ"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="6">
        <location>
            <begin position="13"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="HTH CENPB-type" evidence="7">
        <location>
            <begin position="78"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="543" mass="61608" checksum="76E61A8A6E84FD73" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6Q7</accession>
    <name>A0A4W2E6Q7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6Q7"/>
    <dbReference type="SMR" id="A0A4W2E6Q7"/>
    <dbReference type="STRING" id="30522.A0A4W2E6Q7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017553.1">
        <property type="protein sequence ID" value="ENSBIXP00000032455.1"/>
        <property type="gene ID" value="ENSBIXG00000015062.1"/>
    </dbReference>
    <dbReference type="OMA" id="GRINCGK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35499" checksum="0921E1AA59515261" modified="2019-09-18" version="1">MTGRNQTTILEFLLLGLPIKSEHQNLFYALFLAMYVTTILGNLLILVLICLDPHLHTPMYLFLSNLSFSDLCFSSVTMPKLLQNMQSQDLSIPYAGCLTQMYFFLFFADLGDFLLVAMAYDRYVAICFPLHYTTIMSPRLCLFLVLLPWMLTTFHAMLHTLLMARLHFCEDNVIPHFFCDLSALLKLSCSDTRVNELVIFFVGGLIIIIPFLLIIMSYARIVSSILKVPSAKGICKAFSTCGSHLTVVSLFYGTIIGLYLCPSAHNSTVKETVMSMMYTVVAPMLNPFIYSLRNRDMKGALGRVFCKKKNAFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E821</accession>
    <name>A0A4W2E821_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Retinol dehydrogenase 10</fullName>
            <ecNumber evidence="5">1.1.1.300</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RDH10</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; retinol metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027561181.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000035169.1"/>
        <property type="gene ID" value="ENSBIXG00000018376.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047325.1">
        <property type="protein sequence ID" value="ENSBIXP00005014767.1"/>
        <property type="gene ID" value="ENSBIXG00005018695.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157063"/>
    <dbReference type="OMA" id="KQAMNNN"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:lipid droplet"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:all-trans-retinol dehydrogenase (NAD+) activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:ear development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:embryonic camera-type eye development"/>
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        <property type="term" value="P:retinoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Retinol dehydrogenase 10"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
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    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="341" mass="38087" checksum="D10ABE05E7D11FC6" modified="2019-09-18" version="1">MNIVVEFFVVTFKVLWAFVLAAARWLVRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINTQSNEETAGMVRHIYRDLEAADAAALQAGNGEEEILPHCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVSVLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDYCASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMKAILTDQPMICTPRLMYIVTFMKSILPFEAVVCMYRFLGADKCMYPFIAQRKQATNNNEAKNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E904</accession>
    <name>A0A4W2E904_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">POLR2I</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027370205.1">
        <property type="nucleotide sequence ID" value="XM_027514404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E904"/>
    <dbReference type="SMR" id="A0A4W2E904"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044708.1">
        <property type="protein sequence ID" value="ENSBIXP00000033298.1"/>
        <property type="gene ID" value="ENSBIXG00000002448.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008770.1">
        <property type="protein sequence ID" value="ENSBIXP00005030135.1"/>
        <property type="gene ID" value="ENSBIXG00005010184.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875707"/>
    <dbReference type="CTD" id="5438"/>
    <dbReference type="GeneTree" id="ENSGT00550000075063"/>
    <dbReference type="OMA" id="DTSMVLF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10508">
        <property type="entry name" value="Zn-ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000009">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034012">
        <property type="entry name" value="Zn_ribbon_RPB9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="82"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="125" mass="14523" checksum="6520687BB57EE018" modified="2019-09-18" version="1">MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAW2</accession>
    <name>A0A4W2EAW2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dual-specificity kinase</fullName>
            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAW2"/>
    <dbReference type="SMR" id="A0A4W2EAW2"/>
    <dbReference type="STRING" id="30522.A0A4W2EAW2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026315.1">
        <property type="protein sequence ID" value="ENSBIXP00000036231.1"/>
        <property type="gene ID" value="ENSBIXG00000019651.1"/>
    </dbReference>
    <dbReference type="OMA" id="SPPTWGD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14155">
        <property type="entry name" value="PKc_TESK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000202">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000134">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF3">
        <property type="entry name" value="DUAL SPECIFICITY TESTIS-SPECIFIC PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="57"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="324"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="429"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="630" mass="68106" checksum="C14A8F03CF17689D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDZ5</accession>
    <name>A0A4W2EDZ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytoplasmic dynein 2 heavy chain 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Cytoplasmic dynein 2 heavy chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Dynein cytoplasmic heavy chain 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Dynein heavy chain isotype 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">DYNC2H1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">The cytoplasmic dynein complex 2 is probably composed by a heavy chain DYNC2H1 homodimer and a number of DYNC2LI1 light intermediate chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EDZ5"/>
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        <property type="protein sequence ID" value="ENSBIXP00000030041.1"/>
        <property type="gene ID" value="ENSBIXG00000001014.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
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        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
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        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="cytoplasmic dynein 2 heavy chain 1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049400">
        <property type="entry name" value="DYNC2H1_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF15">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 2 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21264">
        <property type="entry name" value="DYNC2H1_AAA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="12 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0243">Dynein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
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            <end position="1830"/>
        </location>
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    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="1971"/>
            <end position="2127"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="2283"/>
            <end position="2431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="1929"/>
            <end position="1972"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="2936"/>
            <end position="2973"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
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            <end position="3196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="4313" mass="493228" checksum="28098D1E6B18FDF3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EF56</accession>
    <name>A0A4W2EF56_BOBOX</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF56"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="156" mass="16896" checksum="D6F612808F17CBB6" modified="2019-09-18" version="1">MHTQRERVWASPLVRRSAIKRWLGHLYRSVLPGLCLPLDSGPPLPVHPPGTGAEKSGMRPMLEGDLNCTQKCLSYGNVPTTSSAAQTACSSPCFTSAWIPYSLFLPRNARFLFQVGCHLPPAQPLPGSVPGKQRGSGQLRSCHSGCRKVSCVTPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFF4</accession>
    <name>A0A4W2EFF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carboxypeptidase O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CPO</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFF4"/>
    <dbReference type="STRING" id="30522.A0A4W2EFF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045837.1">
        <property type="protein sequence ID" value="ENSBIXP00000034864.1"/>
        <property type="gene ID" value="ENSBIXG00000017969.1"/>
    </dbReference>
    <dbReference type="OMA" id="EEQWAGQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06247">
        <property type="entry name" value="M14_CPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000050">
        <property type="entry name" value="Carboxypeptidase O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033850">
        <property type="entry name" value="Carboxypeptidase_O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF19">
        <property type="entry name" value="CARBOXYPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021242281" description="Carboxypeptidase O" evidence="19">
        <location>
            <begin position="21"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="20">
        <location>
            <begin position="50"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="18">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00058352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="375" mass="43209" checksum="5E7268AD79009F87" modified="2019-09-18" version="1">MKPLLGTFYLLGMLVPGWLGYDRSLTQQRQEVVDKVVSPWSILETYSYNRYHPMGEIYQWMSEIREKYTEVVTQHFLGMTYESRPMYYLKISQPSSNPKKIIWMDCGIHAREWIAPAFCQWFVKEILQNYEDNSRIRRLLKNLDFYVLPVFNIDGYIYTWTTDRLWRKSRSSHNNGTCFGTDLNRNFDASWCSIGASHNCESLTFCGTGPMSEPETKAVSSFIESKKENIACFLTMHSYGQLILVPYGYTKNKSNNHEELIQVGQKAANALKAKHGTNYRVGSSADILYATSGSSRDWARDIGIPFSYTFELRDNGTYGFVLPETQIQATCEETMEAVLSVLDDVYEKYWYTNSARKAKSTALVLGLLMSFMSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFS3</accession>
    <name>A0A4W2EFS3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113875792</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFS3"/>
    <dbReference type="STRING" id="30522.A0A4W2EFS3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023647.1">
        <property type="protein sequence ID" value="ENSBIXP00000035079.1"/>
        <property type="gene ID" value="ENSBIXG00000000696.1"/>
    </dbReference>
    <dbReference type="OMA" id="MCSPVIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF253">
        <property type="entry name" value="OLFACTORY RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="49"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="329" mass="36233" checksum="67D938A5EF629C8C" modified="2019-09-18" version="1">MLFLMERALYLGNMSGVQEFILLGLSARQGVRVVVLALFLPLYLLTLLENALIVFLVCSHTELHKPMYFFLGNLSCLEMCYVSVTMPSLLAGLWTGPYHVPFTACLIQLFLFISLISTKCTLLASMACDRYVAICRPLRYPLLMRPQVCLGLAGTSWLGGLLVSVAKTACIASLSYCGPNVLNHFFCDVSPLLNLSCTHVALTELVDFLSAIVTFCGTLLVALASYSAIGVAVLCMPSATARRKAFSTCASHLVVVGIFYSVALFMYSCPSRVESTDLHKLLSVIYTVVTPACSPVVYCLRNREVHAALFLKETRRWLITYPPNVRPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EI25</accession>
    <name>A0A4W2EI25_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EI25"/>
    <dbReference type="STRING" id="30522.A0A4W2EI25"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017164.1">
        <property type="protein sequence ID" value="ENSBIXP00000032292.1"/>
        <property type="gene ID" value="ENSBIXG00000014845.1"/>
    </dbReference>
    <dbReference type="OMA" id="LMGIWWM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015741">
        <property type="term" value="P:fumarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015729">
        <property type="term" value="P:oxaloacetate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF109">
        <property type="entry name" value="NA(+)_CITRATE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="580" mass="64453" checksum="5C41BC38B8982DF7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKF0</accession>
    <name>A0A4W2EKF0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PLAC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKF0"/>
    <dbReference type="STRING" id="30522.A0A4W2EKF0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022850.1">
        <property type="protein sequence ID" value="ENSBIXP00000034727.1"/>
        <property type="gene ID" value="ENSBIXG00000017802.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033222">
        <property type="entry name" value="PLAC1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14380:SF7">
        <property type="entry name" value="OOCYTE-SECRETED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14380">
        <property type="entry name" value="PLACENTA-SPECIFIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="80" mass="9556" checksum="CD447F89F5D5B2EE" modified="2019-09-18" version="1">MVSVSPCAYRHRYIFADELYLRSGCPMTWIQTYMYDFIYPVYERIKVVSEQALLFQTELYFPPRDIRYGPQKIPLECSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKZ9</accession>
    <name>A0A4W2EKZ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MTHFD2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methenyltetrahydrofolate + NADH</text>
            <dbReference type="Rhea" id="RHEA:22892"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKZ9"/>
    <dbReference type="SMR" id="A0A4W2EKZ9"/>
    <dbReference type="STRING" id="30522.A0A4W2EKZ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025230.1">
        <property type="protein sequence ID" value="ENSBIXP00000035757.1"/>
        <property type="gene ID" value="ENSBIXG00000003365.1"/>
    </dbReference>
    <dbReference type="OMA" id="VCHILTK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000001">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000070">
        <property type="entry name" value="probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF15">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="68"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="202"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="378" mass="40762" checksum="F72D1BB215F10C4A" modified="2019-09-18" version="1">MGAGRRRGLPRCSGITVRPARASFPELTMAAASFITSLVTRLLRSAQSGRLHQRPFHLSAVRNEAVVISGRKLAEQIKQEVRQEVEEWVASGNKRPHLSVVLVGENPASQSYVLNKTRAAASVGINSETILKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERKVCNAVSPDKDVDGFHVINVGRMCLDQCSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEELKKHTALADIVISAAGIPNLITADMIKEGAAVIDVGINRIQDPITAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEQEVLKSKELGVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELX3</accession>
    <name>A0A4W2ELX3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RAB3 GTPase activating non-catalytic protein subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RAB3GAP2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rab3-GAP regulatory subunit family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000047743.1">
        <property type="protein sequence ID" value="ENSBIXP00000037457.1"/>
        <property type="gene ID" value="ENSBIXG00000002988.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026059">
        <property type="entry name" value="Rab3GAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029257">
        <property type="entry name" value="RAB3GAP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032839">
        <property type="entry name" value="RAB3GAP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472:SF0">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472">
        <property type="entry name" value="RAB3-GAP REGULATORY DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14656">
        <property type="entry name" value="RAB3GAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14655">
        <property type="entry name" value="RAB3GAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3-GAP regulatory subunit N-terminal" evidence="7">
        <location>
            <begin position="73"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3GAP regulatory subunit C-terminal" evidence="8">
        <location>
            <begin position="768"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1392" mass="155652" checksum="AF1ADC1AD6A7B032" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EM33</accession>
    <name>A0A4W2EM33_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Mitotic checkpoint serine/threonine-protein kinase BUB1 beta</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">MAD3/BUB1-related protein kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Mitotic checkpoint kinase MAD3L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">BUB1B</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EM33"/>
    <dbReference type="STRING" id="30522.A0A4W2EM33"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029968.1">
        <property type="protein sequence ID" value="ENSBIXP00000037784.1"/>
        <property type="gene ID" value="ENSBIXG00000021463.1"/>
    </dbReference>
    <dbReference type="OMA" id="KTLCPNP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000494">
        <property type="entry name" value="mitotic checkpoint serine/threonine-protein kinase BUB1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.430:FF:000002">
        <property type="entry name" value="mitotic checkpoint serine/threonine-protein kinase BUB1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF25">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE_THREONINE-PROTEIN KINASE BUB1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="21" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="13" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="29">
        <location>
            <begin position="63"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="728"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="248"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="463"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00074450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00080874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120819" checksum="4DB496398CE2FCB7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESW3</accession>
    <name>A0A4W2ESW3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="8">MAD homolog</shortName>
            <shortName evidence="8">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SMAD6</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408842.1">
        <property type="nucleotide sequence ID" value="XM_027553041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESW3"/>
    <dbReference type="SMR" id="A0A4W2ESW3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035039.1">
        <property type="protein sequence ID" value="ENSBIXP00000039995.1"/>
        <property type="gene ID" value="ENSBIXG00000003911.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015830.1">
        <property type="protein sequence ID" value="ENSBIXP00005027826.1"/>
        <property type="gene ID" value="ENSBIXG00005030012.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899775"/>
    <dbReference type="CTD" id="4091"/>
    <dbReference type="GeneTree" id="ENSGT00940000158146"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10493">
        <property type="entry name" value="MH1_SMAD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10499">
        <property type="entry name" value="MH2_SMAD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000004">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000003">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF28">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="10">
        <location>
            <begin position="148"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="11">
        <location>
            <begin position="331"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="497" mass="53684" checksum="D23FB8D062E462F4" modified="2019-09-18" version="1">MFRSKRSGLVRRLWRSRVVPDREEGGGSGGGGGDEDGSVGSRAEPAARAREGGGCGRPEVRPVALRRPRDAVGQRGAQGAGRRRRSGGPPRPMSEPGAGSGGSSLDVGEPGGQGWLPESDCETVTCCLFSERDAAGAPREAGEPLVGAAQEPEGGGRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVLVPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFAAAADGPTVCCNPYHFSRLCGPESPPPPYSRLSPRDEYKPLDLSDSTLSYTETEAANSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLLQHGPEPDAADGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUW3</accession>
    <name>A0A4W2EUW3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC113890373</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EUW3"/>
    <dbReference type="STRING" id="30522.A0A4W2EUW3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050252.1">
        <property type="protein sequence ID" value="ENSBIXP00000040811.1"/>
        <property type="gene ID" value="ENSBIXG00000024971.1"/>
    </dbReference>
    <dbReference type="OMA" id="MATTECF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15419">
        <property type="entry name" value="7tmA_OR9K2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="46"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="316" mass="34849" checksum="D6A2CEBCD2013830" modified="2019-09-18" version="1">MSPHGNGNLSVMTFQEFLLEGFEGGLQTQALLFALFLALYVVAVLGNLTMIVVITLDARLHFPMYFFLKNLSFVDLCYSSVIYPKALANFLSSSKVITFAGCATQFFFFSMLVTTEGFLLALMAYDRFMAICSPLRYPISMCPSVCACLVLGCYCGGSFNSIVQTPLTFTLPFCGSNHIDHFHCDVPPLLKLACADTMTNELVMFGLSGLIIMGTTLVVLVSYGYITVTILRMSSGEGRHKLFSTCGSHMTAMSLFYGTLFVMYAQPGAVESMEQGKVVSVFYTLVIPMLNPLVYSLRNKDVKDALWRLGQKHTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWU9</accession>
    <name>A0A4W2EWU9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Development and differentiation-enhancing factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ASAP3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EWU9"/>
    <dbReference type="STRING" id="30522.A0A4W2EWU9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050629.1">
        <property type="protein sequence ID" value="ENSBIXP00000041302.1"/>
        <property type="gene ID" value="ENSBIXG00000005220.1"/>
    </dbReference>
    <dbReference type="OMA" id="IMEAQLP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051492">
        <property type="term" value="P:regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17900">
        <property type="entry name" value="ArfGap_ASAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07640">
        <property type="entry name" value="BAR_ASAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000002">
        <property type="entry name" value="arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000036">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000002">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047006">
        <property type="entry name" value="ASAP3_ArfGap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028775">
        <property type="entry name" value="BAR_ASAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF1">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="307"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="17">
        <location>
            <begin position="431"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="590"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="626"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="700"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="743"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="794"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="883" mass="96266" checksum="AC756383E2ECA441" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYZ5</accession>
    <name>A0A4W2EYZ5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zic family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZIC4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EYZ5"/>
    <dbReference type="SMR" id="A0A4W2EYZ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039484.1">
        <property type="protein sequence ID" value="ENSBIXP00000041994.1"/>
        <property type="gene ID" value="ENSBIXG00000026152.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013678.1">
        <property type="protein sequence ID" value="ENSBIXP00005028506.1"/>
        <property type="gene ID" value="ENSBIXG00005008199.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154200"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001330">
        <property type="entry name" value="Zinc finger protein ZIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="269" mass="29939" checksum="CF0BB53B2F2D2B0E" modified="2019-09-18" version="1">MRSKTSLVMRKRIRLYRNALKEPSSSSGHHGPQLAAASSPSVFPGLHEQPPQASPSGTLNGLLRLGLPGDMYARPEPFPPGPVSRSDALAAAAALHSYGGMNLTMNLAAPHGPGAFFRYMRQPIKQELICKWLAADGPVPPRLCSKTFSTMHELVTHVTVEHVGGPEQANHICFWEECPRQGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTELLPWRFPQTLLLSNEKREKQQQQQQNVSEMAIDLTTNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2C6</accession>
    <name>A0A4W2F2C6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centromere-associated protein E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centromere protein E</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2F2C6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041687.1">
        <property type="protein sequence ID" value="ENSBIXP00000043197.1"/>
        <property type="gene ID" value="ENSBIXG00000027285.1"/>
    </dbReference>
    <dbReference type="OMA" id="ENECFSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140694">
        <property type="term" value="P:membraneless organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030071">
        <property type="term" value="P:regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000026">
        <property type="entry name" value="Centromere-associated protein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="19"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2481"/>
            <end position="2586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="500"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="722"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="834"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1046"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1120"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1394"/>
            <end position="1906"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1954"/>
            <end position="1988"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="2261"/>
            <end position="2330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="2427"/>
            <end position="2461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2487"/>
            <end position="2500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2511"/>
            <end position="2525"/>
        </location>
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        <location>
            <begin position="94"/>
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        <ligand>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070169"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081766"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2586" mass="303677" checksum="FB73D21FF15827DB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3I9</accession>
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        <recommendedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 5</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228:SF3">
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="35"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 5 helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="843" mass="93138" checksum="95C59790EDCEEC33" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3W0</accession>
    <name>A0A4W2F3W0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="21">Carbonyl reductase (NADPH)</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC113896463</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-nitrosoglutathione + NADPH + H(+) = S-(hydroxysulfenamide)glutathione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 21">
            <text>a secondary alcohol + NADP(+) = a ketone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.184"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>corticosterone + NADPH + H(+) = 20beta-dihydrocorticosterone + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:70219"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16827"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:189050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>cortisol + NADPH + H(+) = 20beta-dihydrocortisol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:70215"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17650"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:139311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:70216"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>daunorubicin + NADPH + H(+) = 13-dihydrodaunorubicin + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64677"/>
            <dbReference type="ChEBI" id="CHEBI:75296"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:63505"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>menadione + NADPH + H(+) = menadiol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63492"/>
            <dbReference type="ChEBI" id="CHEBI:6746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28869"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>prostaglandin D2 + NADP(+) = 15-oxoprostaglandin D2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57406"/>
            <dbReference type="ChEBI" id="CHEBI:57408"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.196"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:20745"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.197"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:11637"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>prostaglandin E2 + NADP(+) = 15-oxoprostaglandin E2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63476"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:63477"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>prostaglandin F2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63481"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>prostaglandin F2alpha + NADP(+) = prostaglandin E2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24508"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="1.1.1.189"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:24510"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 21">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.184" evidence="21"/>
    <dbReference type="RefSeq" id="XP_027404439.1">
        <property type="nucleotide sequence ID" value="XM_027548638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3W0"/>
    <dbReference type="SMR" id="A0A4W2F3W0"/>
    <dbReference type="STRING" id="30522.A0A4W2F3W0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042585.1">
        <property type="protein sequence ID" value="ENSBIXP00000043705.1"/>
        <property type="gene ID" value="ENSBIXG00000027854.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022570.1">
        <property type="protein sequence ID" value="ENSBIXP00005035831.1"/>
        <property type="gene ID" value="ENSBIXG00005017110.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896463"/>
    <dbReference type="GeneTree" id="ENSGT00510000046499"/>
    <dbReference type="OMA" id="YWANDSV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047021">
        <property type="term" value="F:15-hydroxyprostaglandin dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047020">
        <property type="term" value="F:15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050221">
        <property type="term" value="F:prostaglandin E2 9-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000164">
        <property type="entry name" value="Carbonyl reductase [NADPH] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963">
        <property type="entry name" value="CARBONYL REDUCTASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963:SF2">
        <property type="entry name" value="CARBONYL REDUCTASE [NADPH] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 21" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 21" id="KW-0521">NADP</keyword>
    <keyword evidence="6 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 21" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="286" mass="31707" checksum="CC745CD6D66CB9BD" modified="2019-09-18" version="1">MSSSTRVALVTGANKGLGFAIVRDLCRRFPGDVVLTARDEARGRAAVQQLQAEGLSPRFHQLDITDLQSIHALRDFLRKEYGGLDVLVNNAAIAFQLSDPTPTPIKAEMTMKTNFFGTRDICTELLPLMKPQGRVVNMSSGWGFKALESCSPELQQKLRSETITEEELVGLMNKFVEDTKNGVHRKEGWPDNNIYGVAKIGITALSRIQARKLSEQRGGDKILLNACCPGWVRTDMGGSKAFKSLEEGIETPMYLALLPSDAEGPHGQFVHEKKVAKWQFLPEFYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F442</accession>
    <name>A0A4W2F442_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Arrestin domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ARRDC3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401413.1">
        <property type="nucleotide sequence ID" value="XM_027545612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F442"/>
    <dbReference type="SMR" id="A0A4W2F442"/>
    <dbReference type="STRING" id="30522.A0A4W2F442"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052849.1">
        <property type="protein sequence ID" value="ENSBIXP00000044256.1"/>
        <property type="gene ID" value="ENSBIXG00000028428.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056194.1">
        <property type="protein sequence ID" value="ENSBIXP00005046443.1"/>
        <property type="gene ID" value="ENSBIXG00005031257.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895006"/>
    <dbReference type="CTD" id="57561"/>
    <dbReference type="GeneTree" id="ENSGT00940000155411"/>
    <dbReference type="OMA" id="IHAGRHE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031699">
        <property type="term" value="F:beta-3 adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060613">
        <property type="term" value="P:fat pad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031649">
        <property type="term" value="P:heat generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071878">
        <property type="term" value="P:negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120163">
        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031651">
        <property type="term" value="P:negative regulation of heat generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090327">
        <property type="term" value="P:negative regulation of locomotion involved in locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035332">
        <property type="term" value="P:positive regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043588">
        <property type="term" value="P:skin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000005">
        <property type="entry name" value="Arrestin domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000007">
        <property type="entry name" value="Arrestin domain-containing protein 3 mRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF49">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="15">
        <location>
            <begin position="187"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="414" mass="46310" checksum="305B702A4D7919EA" modified="2019-09-18" version="1">MVLGKVKSLTISFDCLNDSNVPVYSSGDTVSGRVNLEVTGEIRVKSLKIHARGHAKVRWTESRNAGSNTAYTQNYTEEVEYFNHKDILIGHERDDDNSEEGFNTIHSGRHEYAFSFELPQTPLATSFEGRHGSVRYWVKAELHRPWLLPVKLKKEFTVFEHIDINTPSLLSPQAGTKEKTLCCWFCTSGPISLSAKIERKGYTPGESIQIFAEIENCSSRMVVPKAAIYQTQAFYAKGKMKEVKQLVANLRGESLSSGKTETWNGKLLKIPPVSPSILDCSIIRVEYSLMVYVDIPGAMDLFLNLPLVIGTIPLHPFGSRTSSVSSQCSMNMNWLGLSLPERPEAPPSYAEVVTEEQRRNNLAPGSACDDFERALQGPLFAYIQEFRFLPPPLYSEIDPNPDQPADDRPSCPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F4D2</accession>
    <name>A0A4W2F4D2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centrosomal protein of 97 kDa</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat and IQ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CEP97</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F4D2"/>
    <dbReference type="STRING" id="30522.A0A4W2F4D2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042997.1">
        <property type="protein sequence ID" value="ENSBIXP00000043941.1"/>
        <property type="gene ID" value="ENSBIXG00000028118.1"/>
    </dbReference>
    <dbReference type="OMA" id="AYWRGFY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000165">
        <property type="entry name" value="Centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000199">
        <property type="entry name" value="centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 97 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="431"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="655"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="703"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="663"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="718"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="734"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="858" mass="95326" checksum="57944C99E86C751F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FD38</accession>
    <name>A0A4W2FD38_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Triggering receptor expressed on myeloid cells 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TREM1</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses. Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12. In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma. This cascade promotes the neutrophil- and macrophage-mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections. By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer. Homomultimer; when activated. Interacts with TYROBP/DAP12. Interacts with TLR4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027381080.1">
        <property type="nucleotide sequence ID" value="XM_027525279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FD38"/>
    <dbReference type="SMR" id="A0A4W2FD38"/>
    <dbReference type="STRING" id="30522.A0A4W2FD38"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003997.1">
        <property type="protein sequence ID" value="ENSBIXP00000030452.1"/>
        <property type="gene ID" value="ENSBIXG00000012434.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010548.1">
        <property type="protein sequence ID" value="ENSBIXP00005003311.1"/>
        <property type="gene ID" value="ENSBIXG00005009467.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882098"/>
    <dbReference type="CTD" id="54210"/>
    <dbReference type="GeneTree" id="ENSGT00470000042299"/>
    <dbReference type="OMA" id="MTDIIRV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070945">
        <property type="term" value="P:neutrophil-mediated killing of gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19357">
        <property type="entry name" value="TRIGGERING RECEPTOR EXPRESSED ON MYELOID CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19357:SF0">
        <property type="entry name" value="TRIGGERING RECEPTOR EXPRESSED ON MYELOID CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611347" description="Triggering receptor expressed on myeloid cells 1" evidence="19">
        <location>
            <begin position="21"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="16"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="232" mass="25495" checksum="E3BC808860C2363C" modified="2019-09-18" version="1">MRKARLWGLLWMLFIEEIQAAAEVFEEKCTLAEGQTLKVSCPTNTNIYSNSQKAWQRLKDNGEVQTLAITEGSSQVRVGKYFLEDIPSEGMLQIQMANLQVEDSGLYRCVILGPSDPIILFHPVRLVVTKNSLGTPASDEYPCQVSVQNPTPLPVTTKLRPRPRPRPKPVTQPIPTSADRLSSPGFTVTPTNVTHVNRAPGISIIIPAACGLLSKTLVFIGLFAVTHRSFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FE54</accession>
    <name>A0A4W2FE54_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wiskott-Aldrich syndrome protein family member</fullName>
            <shortName evidence="7">WASP family protein member</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">WASF2</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds actin and the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375005.1">
        <property type="nucleotide sequence ID" value="XM_027519204.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027375011.1">
        <property type="nucleotide sequence ID" value="XM_027519210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FE54"/>
    <dbReference type="SMR" id="A0A4W2FE54"/>
    <dbReference type="STRING" id="30522.A0A4W2FE54"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047691.1">
        <property type="protein sequence ID" value="ENSBIXP00000016872.1"/>
        <property type="gene ID" value="ENSBIXG00000020956.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010038.1">
        <property type="protein sequence ID" value="ENSBIXP00005003575.1"/>
        <property type="gene ID" value="ENSBIXG00005009713.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878229"/>
    <dbReference type="CTD" id="10163"/>
    <dbReference type="GeneTree" id="ENSGT00950000182962"/>
    <dbReference type="OMA" id="PDMAYND"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001667">
        <property type="term" value="P:ameboidal-type cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072673">
        <property type="term" value="P:lamellipodium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035855">
        <property type="term" value="P:megakaryocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051497">
        <property type="term" value="P:negative regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010592">
        <property type="term" value="P:positive regulation of lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098974">
        <property type="term" value="P:postsynaptic actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016601">
        <property type="term" value="P:Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000012">
        <property type="entry name" value="Wiskott-Aldrich syndrome protein family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF6">
        <property type="entry name" value="ACTIN-BINDING PROTEIN WASF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="236"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="293"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="493" mass="54031" checksum="67DFD8519C2788FE" modified="2019-09-18" version="1">MPLVTRNIEPRHLCRQTLPSVRSELECMTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTVQDQKLFDRNSLPIPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKPSRYPPTLVYQNGSIGSVENVDAGNYPPPPQSDSISPPSPSFSEDNLPPPPAEFSYPADNNQRAGLKRSSVVSPSHPPPAPPLGSAPGPKPGFAPPPAPPPPPPMINTPPPPPPGGFGSPATPPPPSPPSFPPHPDFAAPPPPPPPPAVDYSTLPPPPLSQSAGGAPPPPPPPPPPGPPPPPFSGADGQLAAPPPPLSDTTKPKSSLPPVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDEWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FJT3</accession>
    <name>A0A4W2FJT3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Multidrug resistance-associated protein 1</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
            <ecNumber evidence="14">7.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATP-binding cassette sub-family C member 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Glutathione-S-conjugate-translocating ATPase ABCC1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Leukotriene C(4) transporter</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">ABCC3</name>
    </gene>
    <organism evidence="32 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>2',3'-cGAMP(in) + ATP + H2O = 2',3'-cGAMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:74887"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143093"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>S-[(2E,6E,10E)-geranylgeranyl]-L-glutathione(in) + ATP + H2O = S-[(2E,6E,10E)-geranylgeranyl]-L-glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81611"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:156326"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:81612"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>daunorubicin(in) + ATP + H2O = daunorubicin(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33147"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64677"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:33148"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>prostaglandin A2-S-(R)-glutathione(in) + ATP + H2O = prostaglandin A2-S-(R)-glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81695"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:133768"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:81696"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>prostaglandin A2-S-(S)-glutathione(in) + ATP + H2O = prostaglandin A2-S-(S)-glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:133769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:81700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>sphing-4-enine 1-phosphate(in) + ATP + H2O = sphing-4-enine 1-phosphate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38951"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60119"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:38952"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>vincristine(in) + ATP + H2O = vincristine(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60160"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143658"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60161"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EC" id="7.6.2.3" evidence="14"/>
    <dbReference type="RefSeq" id="XP_027375302.1">
        <property type="nucleotide sequence ID" value="XM_027519501.1"/>
    </dbReference>
    <dbReference type="STRING" id="30522.A0A4W2FJT3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003924.1">
        <property type="protein sequence ID" value="ENSBIXP00000006354.1"/>
        <property type="gene ID" value="ENSBIXG00000012472.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007501.1">
        <property type="protein sequence ID" value="ENSBIXP00005004856.1"/>
        <property type="gene ID" value="ENSBIXG00005010704.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878429"/>
    <dbReference type="CTD" id="8714"/>
    <dbReference type="GeneTree" id="ENSGT00940000161624"/>
    <dbReference type="OMA" id="KTWIMAF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015164">
        <property type="term" value="F:glucuronoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071714">
        <property type="term" value="F:icosanoid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071716">
        <property type="term" value="P:leukotriene transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006855">
        <property type="term" value="P:xenobiotic transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000293">
        <property type="entry name" value="ATP binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000007">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF405">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="528"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="967"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="1014"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="1103"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="1197"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="31">
        <location>
            <begin position="312"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="30">
        <location>
            <begin position="629"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="31">
        <location>
            <begin position="974"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="30">
        <location>
            <begin position="1293"/>
            <end position="1525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1529" mass="169752" checksum="6CD364BB8B24FEC1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FSL7</accession>
    <name>A0A4W2FSL7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SMCR8-C9orf72 complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SMCR8</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SMCR8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375451.1">
        <property type="nucleotide sequence ID" value="XM_027519650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FSL7"/>
    <dbReference type="STRING" id="30522.A0A4W2FSL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002964.1">
        <property type="protein sequence ID" value="ENSBIXP00000006846.1"/>
        <property type="gene ID" value="ENSBIXG00000012921.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016041.1">
        <property type="protein sequence ID" value="ENSBIXP00005008510.1"/>
        <property type="gene ID" value="ENSBIXG00005001655.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878501"/>
    <dbReference type="CTD" id="140775"/>
    <dbReference type="GeneTree" id="ENSGT00390000010052"/>
    <dbReference type="OMA" id="KPVKHWV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902902">
        <property type="term" value="P:negative regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901098">
        <property type="term" value="P:positive regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE PROTEIN SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334">
        <property type="entry name" value="SMITH-MAGENIS SYNDROME REGION GENE 8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="7">
        <location>
            <begin position="48"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="938" mass="104134" checksum="BFA5171760539858" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FT23</accession>
    <name>A0A4W2FT23_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Ras-related protein Rab-28</fullName>
            <ecNumber evidence="6">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RAB28</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB28 is required for shedding and phagocytosis of cone cell outer segments (OS) discs in the retina. Also participates in nuclear factor kappa-B p65/RELA nuclear transport in endothelial cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027400850.1">
        <property type="nucleotide sequence ID" value="XM_027545049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FT23"/>
    <dbReference type="SMR" id="A0A4W2FT23"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034535.1">
        <property type="protein sequence ID" value="ENSBIXP00000020454.1"/>
        <property type="gene ID" value="ENSBIXG00000023765.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016322.1">
        <property type="protein sequence ID" value="ENSBIXP00005008689.1"/>
        <property type="gene ID" value="ENSBIXG00005013804.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894526"/>
    <dbReference type="CTD" id="9364"/>
    <dbReference type="GeneTree" id="ENSGT00940000161833"/>
    <dbReference type="OMA" id="YKNVNLH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04109">
        <property type="entry name" value="Rab28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000513">
        <property type="entry name" value="ras-related protein Rab-28 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-0636">Prenylation</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00039505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00093324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="221" mass="24743" checksum="9DFAC504247D4A46" modified="2019-09-18" version="1">MSDSEEESLDRQLKIVVLGDGTSGKTSLATCFAQETFGKQYKQTIGLDFFLRRITLPGSLNVTLQVWDIGGQTIGGKMLDKYIYGAQGVLLVYDVTNYQSFENLEDWYSVVKKVSEESETQPLVALVGNKIDLEHMRTVKPEKHLRFCQENGFSSHFVSAKTGDSVFLCFQKVAAEILGIKLNKAEIEQSQRVVKADIVNYNQEPMSRTVNPPRSSMCAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FZX3</accession>
    <name>A0A4W2FZX3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Estrogen-induced osteoclastosis regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EEIG1</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FZX3"/>
    <dbReference type="STRING" id="30522.A0A4W2FZX3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027509.1">
        <property type="protein sequence ID" value="ENSBIXP00000015848.1"/>
        <property type="gene ID" value="ENSBIXG00000020216.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045502.1">
        <property type="protein sequence ID" value="ENSBIXP00005011089.1"/>
        <property type="gene ID" value="ENSBIXG00005015589.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156811"/>
    <dbReference type="OMA" id="RPPVKQN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045672">
        <property type="term" value="P:positive regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF2">
        <property type="entry name" value="EARLY ESTROGEN-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="383" mass="41848" checksum="AA37787A6A10D189" modified="2019-09-18" version="1">MAFLMKKKKFKFQTTFTLEELTAVPFVNGVLFCKVRLLDGGDFVSLSSREEVQENCVRWRKRFTFVCKMSANPATGLLDPCIFRVSVRKELKGGKAYSKLGFADLNLAEFAGSGSTVRCCLLEGYDTKNTRQDNSILKVTIGMFLLSGDPCFKTPPSTAKSISIPGQDSSLQLTCKGGGTSRGGSSNSLTGSRPPKARPTILSSGVPEEPDQSLSSPEEVFHSGHSRNSSYASQQSKISGYSTEQSRSSSLSDLTHRRNTSTSSSASGGLSMAVEGPEGSEREHRPPEKPPRPPRPPHLSDRSFRRKKDSVESHPTWVDDTRIDADDIVEKIMQSQDFTEGSNTEDSNLRLFVSRDGTTTLSGIQLANRVSSGVYEPVVIESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GLX0</accession>
    <name>A0A4W2GLX0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucagon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GCG</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucagon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379284.1">
        <property type="nucleotide sequence ID" value="XM_027523483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GLX0"/>
    <dbReference type="SMR" id="A0A4W2GLX0"/>
    <dbReference type="STRING" id="30522.A0A4W2GLX0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038716.1">
        <property type="protein sequence ID" value="ENSBIXP00000023227.1"/>
        <property type="gene ID" value="ENSBIXG00000005273.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049532.1">
        <property type="protein sequence ID" value="ENSBIXP00005018975.1"/>
        <property type="gene ID" value="ENSBIXG00005022246.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880856"/>
    <dbReference type="CTD" id="2641"/>
    <dbReference type="GeneTree" id="ENSGT00390000005372"/>
    <dbReference type="OMA" id="MNTKRNX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031769">
        <property type="term" value="F:glucagon receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071377">
        <property type="term" value="P:cellular response to glucagon stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900118">
        <property type="term" value="P:negative regulation of execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090280">
        <property type="term" value="P:positive regulation of calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035774">
        <property type="term" value="P:positive regulation of insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014823">
        <property type="term" value="P:response to activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.590">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015550">
        <property type="entry name" value="Glucagon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000532">
        <property type="entry name" value="Glucagon_GIP_secretin_VIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11418">
        <property type="entry name" value="GLUCAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11418:SF0">
        <property type="entry name" value="PRO-GLUCAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00123">
        <property type="entry name" value="Hormone_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00275">
        <property type="entry name" value="GLUCAGON"/>
    </dbReference>
    <dbReference type="SMART" id="SM00070">
        <property type="entry name" value="GLUCA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00260">
        <property type="entry name" value="GLUCAGON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611675" evidence="5">
        <location>
            <begin position="21"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="6">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="180" mass="20944" checksum="8D9B4FF05B9F15FF" modified="2019-09-18" version="1">MKSLYFVAGLFVMLVQGSWQRSLQNTEEKSSSFPAPQTDPLGDPDQINEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNKNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVNIVEELRRRHADGSFSDEMNTVLDSLATRDFINWLLQTKITDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GN73</accession>
    <name>A0A4W2GN73_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">5-hydroxytryptamine receptor 3A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">5-hydroxytryptamine receptor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Serotonin receptor 3A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Serotonin-gated ion channel receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">HTR3A</name>
    </gene>
    <organism evidence="31 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Forms homopentameric as well as heteropentameric serotonin-activated cation-selective channel complexes with HTR3B or HTR3C or HTR3D or HTR3E. The homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Heteropentameric complexes display properties which resemble that of neuronal serotonin-activated channels in vivo. Interacts with RIC3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GN73"/>
    <dbReference type="STRING" id="30522.A0A4W2GN73"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008045.1">
        <property type="protein sequence ID" value="ENSBIXP00000029165.1"/>
        <property type="gene ID" value="ENSBIXG00000010699.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032539.1">
        <property type="protein sequence ID" value="ENSBIXP00005019612.1"/>
        <property type="gene ID" value="ENSBIXG00005022765.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157705"/>
    <dbReference type="OMA" id="KFFVVIR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904602">
        <property type="term" value="C:serotonin-activated cation-selective channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099507">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022850">
        <property type="term" value="F:serotonin-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140227">
        <property type="term" value="P:serotonin-gated cation-selective signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19011">
        <property type="entry name" value="LGIC_ECD_5-HT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19063">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000020">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000017">
        <property type="entry name" value="5-hydroxytryptamine receptor 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008132">
        <property type="entry name" value="5HT3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008133">
        <property type="entry name" value="5HT3_rcpt_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049944">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01709">
        <property type="entry name" value="5HT3ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01708">
        <property type="entry name" value="5HT3RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 27" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 27" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 27" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 27" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516536" description="5-hydroxytryptamine receptor 3A" evidence="27">
        <location>
            <begin position="31"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="29">
        <location>
            <begin position="48"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="30">
        <location>
            <begin position="261"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00080492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="490" mass="56005" checksum="0815DA298B364E24" modified="2019-09-18" version="1">ALREATMLPWLPRALLGLLLPTLLAPWGEAKHRGDPQPPNASRPALLRLSDHLLANYKKGVRPVRDWRTPTTVSIDVIVYAILSVDEKNQVLTTYIWYRQYWTDEFLQWDPEDFDNITKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYVRHHGEVQNYKPLQVMTACTLDIYNFPFDIQNCSLTFTSWLHTIQDINISLWRLPEKVKFDRSVFMNQGEWELLGVLTQFQEFSIESSDSYAEMKFYVVIRRRPLFYAVSLLLPSIFLMVVDIVGFYLPPDSGERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETILIVRLVHKQDLQQPVPAWLRHLVLERVTLLLCLGDQSTARRPAATSQAAKTDDCSDMGNHYSHLGGPRDLEKTPRGRGSPPPPPREASLAVRGLLQELTSIRHFLEKREGSREVARDWLRVGSVLDRLLFRIYLLAVLAYSITLVTLWSIWQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GUX8</accession>
    <name>A0A4W2GUX8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GUX8"/>
    <dbReference type="STRING" id="30522.A0A4W2GUX8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030655.1">
        <property type="protein sequence ID" value="ENSBIXP00000017915.1"/>
        <property type="gene ID" value="ENSBIXG00000021825.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036862.1">
        <property type="protein sequence ID" value="ENSBIXP00005022492.1"/>
        <property type="gene ID" value="ENSBIXG00005025371.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214430"/>
    <dbReference type="OMA" id="CWNTEVE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00095">
        <property type="entry name" value="IFab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000001">
        <property type="entry name" value="Interferon alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF37">
        <property type="entry name" value="INTERFERON OMEGA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00266">
        <property type="entry name" value="INTERFERONAB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00076">
        <property type="entry name" value="IFabd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00252">
        <property type="entry name" value="INTERFERON_A_B_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2 6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611715" evidence="7">
        <location>
            <begin position="24"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="186" mass="20961" checksum="C6F74B21C988F949" modified="2019-09-18" version="1">MACVLSLLMALVLVSYGPGGSLGCDLSQNHVLVGRQNLRLLGQMRRLSPRFCLQDRKDFAFPQEMVEGGQLHEAQAISVLHEILQQTFNLFHTERSSAAWDTTLLEQLHTGLHQQLDDLDACLGQVMGEEDSALGRTGPTLAVKRYFQGIHVYLKEKEYSDCAWEIVIMEIMRSLSSSTNLQERLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H1W7</accession>
    <name>A0A4W2H1W7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pleckstrin homology domain-containing family F member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PLEKHF1</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027371851.1">
        <property type="nucleotide sequence ID" value="XM_027516050.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371852.1">
        <property type="nucleotide sequence ID" value="XM_027516051.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371853.1">
        <property type="nucleotide sequence ID" value="XM_027516052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H1W7"/>
    <dbReference type="SMR" id="A0A4W2H1W7"/>
    <dbReference type="STRING" id="30522.A0A4W2H1W7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019475.1">
        <property type="protein sequence ID" value="ENSBIXP00000010587.1"/>
        <property type="gene ID" value="ENSBIXG00000016040.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052779.1">
        <property type="protein sequence ID" value="ENSBIXP00005025003.1"/>
        <property type="gene ID" value="ENSBIXG00005027531.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876623"/>
    <dbReference type="CTD" id="79156"/>
    <dbReference type="GeneTree" id="ENSGT00940000160728"/>
    <dbReference type="OMA" id="DDKADQW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001244">
        <property type="term" value="P:positive regulation of intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01218">
        <property type="entry name" value="PH_Phafin2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000247">
        <property type="entry name" value="pleckstrin homology domain-containing family F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000284">
        <property type="entry name" value="pleckstrin homology domain-containing family F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051765">
        <property type="entry name" value="PH_domain-containing_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037871">
        <property type="entry name" value="PH_Phafin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280:SF2">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="35"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="16">
        <location>
            <begin position="152"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="219"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="279" mass="31003" checksum="71941479C3703494" modified="2019-09-18" version="1">MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLETLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLLATGLQPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRERFLLPRLSSKPLRVCSLCFRELAAQKRKEDVEEQGLGSPGPSAHLAGAVCGASSGDDDDSDEDREGSGDGDWPSHVEFYASGVSWSSFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H2D6</accession>
    <name>A0A4W2H2D6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PSMB4</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with PRPF19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
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        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
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        <property type="entry name" value="Proteasome_bsu_CS"/>
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        <property type="entry name" value="METALLOPROTEASE TLDD"/>
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        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
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        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025941.1"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="264" mass="29031" checksum="B2C4029A25FCC76A" modified="2019-09-18" version="1">MEALLESRSGLWAGGPAPGQFYRIPPTPGSSVDPVSALYGSPITRTQNPMVTGTSVLGLKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARELVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSAETNWDIAHMISGFE</sequence>
</entry>
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    <accession>A0A4W2H3L0</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="project" value="Ensembl"/>
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        <location>
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            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="323"/>
            <end position="520"/>
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            <end position="162"/>
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    </feature>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="595" mass="66586" checksum="63F2056CA7499B2D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H6U5</accession>
    <name>A0A4W2H6U5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H6U5"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000015228.1">
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        <property type="gene ID" value="ENSBIXG00000029250.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005027667.1"/>
        <property type="gene ID" value="ENSBIXG00005030304.1"/>
    </dbReference>
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    <dbReference type="OMA" id="MESHHAH"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051306">
        <property type="entry name" value="Homeobox_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123:SF3">
        <property type="entry name" value="DOUBLE HOMEOBOX PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="88"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="90"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="428" mass="44533" checksum="26E7118D47E027C6" modified="2019-09-18" version="1">MARARVSPEKPGSRRRRLVLKPSQKDALQALFQQNPYPGIATRERLARELGIDESRVQVWFQNQRRRRSKQSRPPSEHVRQEGEGGPTEARRKRTVISPSQTRILVQAFTRDRFPGIAAREELARQTGIPEPRIQIWFQNRRARHPQRSPSGPGNGRAQGPGGAPATTTTPAPEDRRAPPAPLMIFVVQPSPAALQPSGRPPPPPQGAAPWAACSPAVTAPGLPGQGAILPPGQPETHIPRWPESPSGEGTAPPLEPQPQAPSLPSSTSLLDELLAATGVPDTQAPSPGAAADEGVGPALPGAPSFLDELLAATGTPDTPGPSLGPSADERAHLALPGELLAAAGLPGSPGPSPGSSPVVAGPHPALPGPPSLLEEILAATAIQDTPWSSPGSPAGEEGVEATLETPLSEDEYQALLDMLPGSPGPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HFT0</accession>
    <name>A0A4W2HFT0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">T-cell-specific transcription factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TCF7</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402297.1">
        <property type="nucleotide sequence ID" value="XM_027546496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HFT0"/>
    <dbReference type="SMR" id="A0A4W2HFT0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038600.1">
        <property type="protein sequence ID" value="ENSBIXP00000023141.1"/>
        <property type="gene ID" value="ENSBIXG00000025712.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008877.1">
        <property type="protein sequence ID" value="ENSBIXP00005030098.1"/>
        <property type="gene ID" value="ENSBIXG00005010211.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895368"/>
    <dbReference type="CTD" id="6932"/>
    <dbReference type="GeneTree" id="ENSGT00940000159831"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000009">
        <property type="entry name" value="transcription factor 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF33">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="14">
        <location>
            <begin position="304"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="12">
        <location>
            <begin position="304"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="374"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="419" mass="45131" checksum="D1A6B0AB7477F805" modified="2019-09-18" version="1">MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPERDLAELKSSLVNESEGAAGDAGVPGAGAGARGEAEVGAEALGREHTSQRLFPDKLPESLEDGLKAPECTSGMYKDTVYSAFNLLMHYPPPSGAGQHPQPQPPLHNKASQPSHGVPQLSPLYDHFSSPHPTPAPADISQKQGVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWPSPPLYPLSPSCGYRQHFPAPTAAPGAPYPRFTHPPLMLGSGVPGHPAAIPHPAIVPSSGKQELQPYDRSLKAQADSKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESNSGGKRNAFGTYPEKAAAPAPFLPMTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HG07</accession>
    <name>A0A4W2HG07_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acylamino-acid-releasing enzyme</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Acyl-peptide hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Acylaminoacyl-peptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">APEH</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Also, involved in the degradation of oxidized and glycated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2HG07"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008181.1">
        <property type="protein sequence ID" value="ENSBIXP00000004270.1"/>
        <property type="gene ID" value="ENSBIXG00000010637.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005438.1">
        <property type="protein sequence ID" value="ENSBIXP00005031206.1"/>
        <property type="gene ID" value="ENSBIXG00005011475.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013172"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000047">
        <property type="entry name" value="Acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000043">
        <property type="entry name" value="acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="15">
        <location>
            <begin position="48"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="521"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="735" mass="81587" checksum="0A3AF8F29E8ECDE4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HGV4</accession>
    <name>A0A4W2HGV4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA polymerase epsilon subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA polymerase II subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">POLE4</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4. Interaction with POLE3 is a prerequisite for further binding with POLE and POLE2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027554792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HGV4"/>
    <dbReference type="SMR" id="A0A4W2HGV4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046616.1">
        <property type="protein sequence ID" value="ENSBIXP00000014647.1"/>
        <property type="gene ID" value="ENSBIXG00000019281.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004572.1">
        <property type="protein sequence ID" value="ENSBIXP00005031516.1"/>
        <property type="gene ID" value="ENSBIXG00005011846.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901002"/>
    <dbReference type="CTD" id="56655"/>
    <dbReference type="GeneTree" id="ENSGT00940000160888"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22929">
        <property type="entry name" value="HFD_POLE4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA polymerase epsilon 4, accessory subunit"/>
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    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF79">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
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    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
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        <location>
            <begin position="39"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="116" mass="12065" checksum="D6E1A72411F97B55" modified="2019-09-18" version="1">MAAAAPGSGAAREEEGTGGDAATPQPPAPTSAPGARLSRLPLARVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HJR8</accession>
    <name>A0A4W2HJR8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Colipase like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CLPSL2</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HJR8"/>
    <dbReference type="STRING" id="30522.A0A4W2HJR8"/>
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        <property type="protein sequence ID" value="ENSBIXP00000005764.1"/>
        <property type="gene ID" value="ENSBIXG00000011899.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005157.1">
        <property type="protein sequence ID" value="ENSBIXP00005031289.1"/>
        <property type="gene ID" value="ENSBIXG00005005817.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011494"/>
    <dbReference type="OMA" id="SDCCLMD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032094">
        <property type="term" value="P:response to food"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Colipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10041">
        <property type="entry name" value="COLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10041:SF3">
        <property type="entry name" value="COLIPASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51342">
        <property type="entry name" value="COLIPASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611902" evidence="1">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="93" mass="10133" checksum="3F6E1134B9214B85" modified="2019-09-18" version="1">MALAIALLLSGVLLPCWGEFALFKKGCSHHSECFSDCCLINLDSGGAFCAPRARITMTCLPQTRSAINIVCPCRVGLKCIQKDPDCSRRCRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HWQ0</accession>
    <name>A0A4W2HWQ0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 262</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM262</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSBIXP00000028404.1"/>
        <property type="gene ID" value="ENSBIXG00000009557.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019077.1">
        <property type="protein sequence ID" value="ENSBIXP00005034298.1"/>
        <property type="gene ID" value="ENSBIXG00005015194.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064783"/>
    <dbReference type="OMA" id="QYREENH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036128">
        <property type="term" value="C:CatSper complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040431">
        <property type="entry name" value="TM262"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37998:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED AUXILIARY SUBUNIT TMEM262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37998">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="116" mass="13873" checksum="800C42AC7300E90A" modified="2019-09-18" version="1">MRWRDRVAVLFFPQGMILTMAALMLFFIHLAVFASDVHNFWVTYRYDRMSFRYTVVLMFSQVISICWAAMGSLYAEMTYDKFLRCFSLTILMLNGAMFFNRLSLEFLAIQYREESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HXK5</accession>
    <name>A0A4W2HXK5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Anaphase promoting complex subunit 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HXK5"/>
    <dbReference type="STRING" id="30522.A0A4W2HXK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052337.1">
        <property type="protein sequence ID" value="ENSBIXP00000025637.1"/>
        <property type="gene ID" value="ENSBIXG00000027716.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027358.1">
        <property type="protein sequence ID" value="ENSBIXP00005037882.1"/>
        <property type="gene ID" value="ENSBIXG00005019879.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000938"/>
    <dbReference type="OMA" id="EGTDQDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026182">
        <property type="entry name" value="ANAPC15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22526">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22526:SF2">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15243">
        <property type="entry name" value="ANAPC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15133" checksum="3B0A701280F50B2D" modified="2025-10-08" version="2">MIPGLLRAMSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSIAEKDNNLVPIGKPASEHYDDEEEEDEDDDEDSEEDSEDDEDMQDMDEMNDYNESPDDGEVNEVDMEGNEQDQDQWMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HY95</accession>
    <name>A0A4W2HY95_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 22">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="18 22">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">USP13</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with UFD1. Interacts (via UBA domains) with SIAH2 (when ubiquitinated). Interacts with BAG6; the interaction is direct and may mediate UBL4A deubiquitination. Interacts (via UBA 2 domain) with AMFR; the interaction is direct. Interacts with UBL4A; may be indirect via BAG6. Interacts with NEDD4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18 22">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="18 22"/>
    <dbReference type="RefSeq" id="XP_027396139.1">
        <property type="nucleotide sequence ID" value="XM_027540338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HY95"/>
    <dbReference type="SMR" id="A0A4W2HY95"/>
    <dbReference type="STRING" id="30522.A0A4W2HY95"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035528.1">
        <property type="protein sequence ID" value="ENSBIXP00000021075.1"/>
        <property type="gene ID" value="ENSBIXG00000024252.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023815.1">
        <property type="protein sequence ID" value="ENSBIXP00005036347.1"/>
        <property type="gene ID" value="ENSBIXG00005017875.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891879"/>
    <dbReference type="CTD" id="8975"/>
    <dbReference type="GeneTree" id="ENSGT00940000157401"/>
    <dbReference type="OMA" id="ASTECAY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904288">
        <property type="term" value="F:BAT3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036506">
        <property type="term" value="P:maintenance of unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035523">
        <property type="term" value="P:protein K29-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044313">
        <property type="term" value="P:protein K6-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14384">
        <property type="entry name" value="UBA1_UBP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000047">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000770">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000042">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000063">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF105">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 13"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
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    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="7 18 22" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11 18 22" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14 18 20" id="KW-0862">Zinc</keyword>
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        <location>
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            <end position="295"/>
        </location>
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            <end position="861"/>
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            <end position="693"/>
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        <location>
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            <end position="767"/>
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        <location>
            <position position="345"/>
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    </feature>
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        <location>
            <position position="823"/>
        </location>
    </feature>
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        <location>
            <position position="211"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="214"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00064879"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU366025"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="863" mass="97278" checksum="04357616964550B5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I4Z0</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="211"/>
            <end position="271"/>
        </location>
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            <begin position="213"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
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    </feature>
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            <begin position="34"/>
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            <end position="79"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="338" mass="36922" checksum="69345E12FAA333DB" modified="2019-09-18" version="1">MTQSLSGPRTLGKVSVRREAPGTPWPRRNGDPAASPPSPAQPFRPPRMEASASAQTPHPHEPISFGIDQILNSPDQDSASAPRGPDGASYLGGPPGGRPGATYPSLPTSFAGLGAPFEDAGSYSVNLSLAPAGVIRVPAHRPLPGAVPPPLPSALPAMPSVPTVSSLGGLNFPWMESSRRFVKDRFTAAAALTPFTVTRRIGHPYQNRTPPKRKKPRTSFSRVQICELEKRFHRQKYLASAERAALAKSLKMTDAQVKTWFQNRRTKWRRQTAEEREAERQQASRLMLQLQHDAFQKSLNDSIQPDPLCLHNSSLFALQNLQPWEEDSSKVPAVTSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I564</accession>
    <name>A0A4W2I564_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Staphylococcal nuclease domain-containing protein</fullName>
            <ecNumber evidence="7">3.1.31.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SND1</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Endonuclease that mediates miRNA decay of both protein-free and AGO2-loaded miRNAs. As part of its function in miRNA decay, regulates mRNAs involved in G1-to-S phase transition. Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for STAT5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.31.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a ternary complex with STAT6 and POLR2A. Associates with the RNA-induced silencing complex (RISC). Interacts with the RISC components AGO2, FMR1 and TNRC6A. Interacts with GTF2E1 and GTF2E2. Interacts with PIM1. Interacts with STAT5. Interacts with SYT11 (via C2 2 domain); the interaction with SYT11 is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="RefSeq" id="XP_027394881.1">
        <property type="nucleotide sequence ID" value="XM_027539080.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2I564"/>
    <dbReference type="STRING" id="30522.A0A4W2I564"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028490.1">
        <property type="protein sequence ID" value="ENSBIXP00000037155.1"/>
        <property type="gene ID" value="ENSBIXG00000020687.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033172.1">
        <property type="protein sequence ID" value="ENSBIXP00005040489.1"/>
        <property type="gene ID" value="ENSBIXG00005023220.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891253"/>
    <dbReference type="CTD" id="27044"/>
    <dbReference type="GeneTree" id="ENSGT00510000047270"/>
    <dbReference type="OMA" id="ARCADHH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097433">
        <property type="term" value="C:dense body"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:RNAi effector complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:3' overhang single-stranded DNA endodeoxyribonuclease activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:RISC complex binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:miRNA catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:mRNA catabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010564">
        <property type="term" value="P:regulation of cell cycle process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00175">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd20433">
        <property type="entry name" value="Tudor_TDRD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000047">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000001">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000002">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000003">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000004">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000005">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047386">
        <property type="entry name" value="Tudor_TDRD11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="9">
        <location>
            <begin position="193"/>
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        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="9">
        <location>
            <begin position="341"/>
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        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="9">
        <location>
            <begin position="525"/>
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        </location>
    </feature>
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        <location>
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            <end position="787"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001544"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045880"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046953"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="910" mass="101989" checksum="E4EC29351E39BE73" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="68" mass="7978" checksum="FB6D04CA8E405DC1" modified="2019-09-18" version="1">MEVDAASEGGRDGPRERRGFGEAERQQQNHEVRSQSGAAVSPKHSYWLDLWLFILFDVIWFFFVYFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BLR3</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSBIXP00005030152.1"/>
        <property type="gene ID" value="ENSBIXG00005010244.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
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            <end position="206"/>
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        <source>
            <dbReference type="PROSITE" id="PS50054"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="217" mass="24812" checksum="1219B346355365BA" modified="2019-09-18" version="1">MTGLVLCEPTELYNILNQVTKLSRLTEPNYLCLLDVRSKREYDESHVITALLVKKELDAFQPYPIEIMPGRIYLGNFKQACDPKIQKDLKISAHVNISMETGPFFVGDADKLLHIQIEDSLEANITPFLRHLCHFIDIHLELNSVILVFSTLGISRSCAAILAYLMHWNGQTLKKSWAYLKKCKNNMRPNRALVAQLSEWEKVVLGDIVTDIQNPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMJ7</accession>
    <name>A0A4W2BMJ7_BOBOX</name>
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        <submittedName>
            <fullName evidence="5">Transforming growth factor beta regulator 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">TBRG1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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            <end position="154"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="411" mass="45120" checksum="D899ACB689CA10DB" modified="2019-09-18" version="1">MSLLGSLASSPRDPLQSSKARMKKLPKKSQNEKYRLKYLRLRRAAKATVFENAAICDEIARLEEKFLKAKEERRYLLKKLLQLQALTEGDVQAAAPSHSSSLPLTYTVASSVGTLQGTGPVSGPSTGAEEPFGKKSKKEKKEKGKENNKLEVLKKTSKKKKMEAGARKPVQPIALDPSGRPVFPIGLGGLTVYSLGEIITDRPGFHDETAIYPVGYCSTRVYASMRSPDQECLYTCQIKDGGTQPQFEIVPEDDPQNAIISSSADACHAELLKTISTAMGKLIPNLHPSGADFFGFSHPAIHNLIQSCPGARKCVNYQWVKFDVCKPGDSPPPQEQLENDIATSFEAFQRHTFDEDHSDPILQGSLDLPELQPTAFVSSYQPMFLTHEPLVDAHLQHLKSPSPCSPSQSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNW0</accession>
    <name>A0A4W2BNW0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyribonuclease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNase I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNW0"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000053227.1">
        <property type="protein sequence ID" value="ENSBIXP00000000399.1"/>
        <property type="gene ID" value="ENSBIXG00000029872.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:programmed cell death involved in cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DNase1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Deoxyribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018057">
        <property type="entry name" value="Deoxyribonuclease-1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016202">
        <property type="entry name" value="DNase_I"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11371">
        <property type="entry name" value="DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11371:SF32">
        <property type="entry name" value="DEOXYRIBONUCLEASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000988">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00130">
        <property type="entry name" value="DNASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00476">
        <property type="entry name" value="DNaseIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00918">
        <property type="entry name" value="DNASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021231120" description="Deoxyribonuclease" evidence="11">
        <location>
            <begin position="21"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Essential for enzymatic activity" evidence="9">
        <location>
            <begin position="194"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007359"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000988-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000988-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="305" mass="34936" checksum="206CAA83AEC54961" modified="2019-09-18" version="1">MPLPLACLLLLLLSTHSALALKICSFNVRSFGESKKANCNAMDVIVKVIKRCDIILLMEIKDSSNRICPTLMEKLNGNSRKGITYNYVISSRLGRNTYKEQYAFLYKEKLVSVKQSYLYHDYQAGDADVFSREPFVVWFQSPYTAVKDFVIVPLHTTPETSVREIDELADVYTDVKRRWNAENFIFMGDFNAGCSYVPKKAWKDIRLRTDPKFVWLIGDQEDTTVKKSTNCAYDRIVLRGQNIVNSVVPQSNLVFDFQKAYRLSESKALDVSDHFPVEFKLQSSRAFTNSKKSVSSKKKKKTSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUM8</accession>
    <name>A0A4W2BUM8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUM8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010831.1">
        <property type="protein sequence ID" value="ENSBIXP00000002973.1"/>
        <property type="gene ID" value="ENSBIXG00000009396.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330">
        <property type="entry name" value="P300 TRANSCRIPTIONAL COFACTOR JMY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330:SF9">
        <property type="entry name" value="PROLINE-RICH PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="364" mass="40254" checksum="110189B508673A42" modified="2019-09-18" version="1">MPKFKQRRRKLKAKAKRLFKRKEASHLQSKLVTPPPPPPSPERAVIPSTDIPISRSWLRLSWNFRFPSFTNAIKLLTNRVWSIYSWCQSCMTQSFEVLKNTIFPSRLCHRELRSLKQRFCVLENEVCKLQEALKAISENSSCTSCGQACHTGGEHPDVPACALASPADSAALRPPSPAQPASRPPPPLPPPPPPPPLPPPPPPTAPLLLRKPALTKALQAGPLKKNGPMQITVKDLLTVKLKKTQSLDERRKLVPSPKAQKALVTISDLQRVTLKPKSKALSTRVTNVLITPGKSQLDLRKLLRKVDVERSPGGTPLTNKENVETGTGLTPVMTQALKRKFQLAHPRSPPQTLPVSTSSFDEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BW54</accession>
    <name>A0A4W2BW54_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22 24">E3 ubiquitin-protein ligase CBL</fullName>
            <ecNumber evidence="7 24">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">CBL</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 24">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="24">The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7 24"/>
    <dbReference type="RefSeq" id="XP_027419291.1">
        <property type="nucleotide sequence ID" value="XM_027563490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BW54"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009765.1">
        <property type="protein sequence ID" value="ENSBIXP00000003484.1"/>
        <property type="gene ID" value="ENSBIXG00000009905.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041879.1">
        <property type="protein sequence ID" value="ENSBIXP00005025949.1"/>
        <property type="gene ID" value="ENSBIXG00005028390.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905758"/>
    <dbReference type="CTD" id="867"/>
    <dbReference type="GeneTree" id="ENSGT00940000155772"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042059">
        <property type="term" value="P:negative regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16708">
        <property type="entry name" value="RING-HC_Cbl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09920">
        <property type="entry name" value="SH2_Cbl-b_TKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14393">
        <property type="entry name" value="UBA_c-Cbl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000030">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000154">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024162">
        <property type="entry name" value="Adaptor_Cbl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014741">
        <property type="entry name" value="Adaptor_Cbl_EF_hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003153">
        <property type="entry name" value="Adaptor_Cbl_N_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014742">
        <property type="entry name" value="Adaptor_Cbl_SH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024159">
        <property type="entry name" value="Cbl_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007">
        <property type="entry name" value="CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02262">
        <property type="entry name" value="Cbl_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02761">
        <property type="entry name" value="Cbl_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02762">
        <property type="entry name" value="Cbl_N3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47668">
        <property type="entry name" value="N-terminal domain of cbl (N-cbl)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51506">
        <property type="entry name" value="CBL_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
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        </location>
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        </location>
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        </location>
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    <sequence length="912" mass="100528" checksum="F3559A201F0EE263" modified="2019-09-18" version="1"> </sequence>
</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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</entry>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="11">Microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. Probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes.</text>
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        </subcellularLocation>
    </comment>
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        <text evidence="2 12">Belongs to the DP1 family.</text>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TB2_DP1_HVA22"/>
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    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF39">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 3"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="55"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="255" mass="29251" checksum="22A9E4E5993C9B5A" modified="2019-09-18" version="1">MVSWIISRAVVLVFGMLYPAYYSYKAVKTKNVKEYVRWMMYWIVFALYTVIETVADQTVAWFPLYYELKIAFVIWLLSPYTRGASLIYRKFLHPLLSSKEREIDDYIVQAKERGYETMVNFGRQGLNLAATAAVTAAVKSQGAITERLRSFSMHDLTAIQGDEPVGQRSYQPLPETKKKSKPGSGETAGYGIPLRDGDEKTDEEAEGPYSDDEMLTHKGLRRSQSMKSVKTVKGRKEVRYGSLKYKVKKRPQVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C078</accession>
    <name>A0A4W2C078_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myopalladin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MYPN</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with TTN/titin, NEB, NEBL, ACTN2 and CARP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the myotilin/palladin family.</text>
    </comment>
    <dbReference type="SMR" id="A0A4W2C078"/>
    <dbReference type="STRING" id="30522.A0A4W2C078"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006883.1">
        <property type="protein sequence ID" value="ENSBIXP00000004889.1"/>
        <property type="gene ID" value="ENSBIXG00000011189.1"/>
    </dbReference>
    <dbReference type="OMA" id="DEMDHKP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20972">
        <property type="entry name" value="IgI_2_Titin_Z1z2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000256">
        <property type="entry name" value="myopalladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000399">
        <property type="entry name" value="myopalladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000761">
        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001108">
        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF13">
        <property type="entry name" value="MYOPALLADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="269"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="435"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="945"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="1073"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="1172"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="18"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="93"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="168"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="361"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="554"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="770"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="850"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="770"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1320" mass="145224" checksum="E2F0570BF6C183C8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1F5</accession>
    <name>A0A4W2C1F5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C1F5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003472.1">
        <property type="protein sequence ID" value="ENSBIXP00000006580.1"/>
        <property type="gene ID" value="ENSBIXG00000012671.1"/>
    </dbReference>
    <dbReference type="OMA" id="HSICRQI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="98"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="430" mass="49069" checksum="038D3B9D47109116" modified="2019-09-18" version="1">MASIKDRNGMDLIEAEDIKKRWQEYTEELHKKDLHDPDNHKGMITPTYLKPDILECEVRWVLGSITTNKASGGDGIPVELLQILKDDTVKVLHSICRQIWKTQQWPQDWKRSVFISIPKKGNLKECSNYCIIALISHASKVMLKILQARLQQYVNREFPDAQAGFRKGRGTRGQIANIRWIMEKAREFQKNIYFCFIDYAKALDCVDHNKLWKILKEMGIPDHLTCLLRNLYTGQEATVSTGHGPTDWFQIGKGVHQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKTAGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASSPITSWEINGEIVETEADFIFLGSKITADGDCSHEIKRRLLLGSKVMTNLDSIFKSRDITLSTKVRLVKAMVFLVVMYGCESWTIKKAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1G4</accession>
    <name>A0A4W2C1G4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-defensin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C1G4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053827.1">
        <property type="protein sequence ID" value="ENSBIXP00000000098.1"/>
        <property type="gene ID" value="ENSBIXG00000030263.1"/>
    </dbReference>
    <dbReference type="OMA" id="RIYSMRK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031731">
        <property type="term" value="F:CCR6 chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.360.10:FF:000001">
        <property type="entry name" value="Beta-defensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.360.10">
        <property type="entry name" value="Antimicrobial Peptide, Beta-defensin 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001855">
        <property type="entry name" value="Defensin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20515">
        <property type="entry name" value="BETA-DEFENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20515:SF2">
        <property type="entry name" value="DEFENSIN BETA 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00711">
        <property type="entry name" value="Defensin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57392">
        <property type="entry name" value="Defensin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0211">Defensin</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Beta-defensin-like" evidence="7">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="62" mass="7009" checksum="DEFAD7D687175882" modified="2019-09-18" version="1">SQCCFPADTFFPLSSLFRIYSMRKIPLSCCRNIGICVPIKCSGNMRQIGTCLGAQVKCCRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2D6</accession>
    <name>A0A4W2C2D6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pecanex-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PCNX2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the pecanex family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000004787.1">
        <property type="protein sequence ID" value="ENSBIXP00000005924.1"/>
        <property type="gene ID" value="ENSBIXG00000012065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039797">
        <property type="entry name" value="Pecanex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007735">
        <property type="entry name" value="Pecanex_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12372">
        <property type="entry name" value="PECANEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12372:SF5">
        <property type="entry name" value="PECANEX-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05041">
        <property type="entry name" value="Pecanex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="829"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="854"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="891"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="938"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1016"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1110"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="Pecanex C-terminal" evidence="8">
        <location>
            <begin position="1606"/>
            <end position="1830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="92"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="429"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="578"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="708"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1861"/>
            <end position="1992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="585"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="732"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1875"/>
            <end position="1889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1890"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1923"/>
            <end position="1936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2119" mass="234965" checksum="2320659C5C5CD0E5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3H8</accession>
    <name>A0A4W2C3H8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3H8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002061.1">
        <property type="protein sequence ID" value="ENSBIXP00000007330.1"/>
        <property type="gene ID" value="ENSBIXG00000013299.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="466" mass="53579" checksum="CBD0011654658EBF" modified="2019-09-18" version="1">MGSIKDRNGMNLTEAEDIKKRWQEYTEELYKKDLHDLDNHDDVITDLEPDILECEVKWALESIATNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGIRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILQEMGIPDHLICLLRNLYAGQEARVRTGHGTTDWFQIGKGVCQGCILSPCLFKLYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLTAESKEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGEIVSDFIFLGSKITADGDCSHEIKRRLFLGRKVMINLDSIFKSRDITLPTKVHLVKAVVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKISPGISLEGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5F5</accession>
    <name>A0A4W2C5F5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 121B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM121B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM121 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399190.1">
        <property type="nucleotide sequence ID" value="XM_027543389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5F5"/>
    <dbReference type="STRING" id="30522.A0A4W2C5F5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002646.1">
        <property type="protein sequence ID" value="ENSBIXP00000007030.1"/>
        <property type="gene ID" value="ENSBIXG00000013052.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035761.1">
        <property type="protein sequence ID" value="ENSBIXP00005021745.1"/>
        <property type="gene ID" value="ENSBIXG00005024700.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893385"/>
    <dbReference type="CTD" id="27439"/>
    <dbReference type="GeneTree" id="ENSGT00940000154822"/>
    <dbReference type="OMA" id="HRVVSNQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026624">
        <property type="entry name" value="CECR6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032776">
        <property type="entry name" value="CECR6/TMEM121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47399">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47399:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14997">
        <property type="entry name" value="CECR6_TMEM121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="506" mass="51344" checksum="2EF17F31925457EA" modified="2019-09-18" version="1">MRPAPGAPRAAPPPARRQPRFLRGRSGSGSSGGPGGSSDSSAGAEREDDDESASISRPLVSAEPPGTPAASRASTPTSARSMTAADLGAGAGAGAVGGAGGPGPGPGPSCRSCCGCCGRRARSGRGGGRRGCSSGSGCRWGYQALSVALLLAQGGLLDVYLIAVTDLYWCSWVATDLVVAAGWAIFFAKNSRGRRGGAHAHHPHHPHAAPLHLPAAPAGAAGAKARGARGGAGGPGPAGPVGAAGEFAFAYLAWLIYSIAFTPKVVLILGTSILDLIELRAPFGTTGFRLTMALSAPLLYCLVRAIGEAGATPGSAGPLLLQPQRHRAAGCFLGTCLDLLDSFALVELMLDGRAPLPAHLRYLLLAVYFLALASPVLWLYELHAAASPRARASRPGGCSCLLSLLGSCLVDAPLLALRCLLALSYQQPLSVFMLKNLFFLGCRGLEALEGCWDQGLTASPSRARSGYGAPPSAPPAPGAPQLGHCISEDEGCAHGYVNTLAVASQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5P5</accession>
    <name>A0A4W2C5P5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">DNA nucleotidylexotransferase</fullName>
            <ecNumber evidence="4 14">2.7.7.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DNTT</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.31" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5P5"/>
    <dbReference type="STRING" id="30522.A0A4W2C5P5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002873.1">
        <property type="protein sequence ID" value="ENSBIXP00000006899.1"/>
        <property type="gene ID" value="ENSBIXG00000012965.1"/>
    </dbReference>
    <dbReference type="OMA" id="PKVINLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003912">
        <property type="term" value="F:DNA nucleotidylexotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18443">
        <property type="entry name" value="BRCT_DNTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.210.10:FF:000003">
        <property type="entry name" value="DNA nucleotidylexotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000028">
        <property type="entry name" value="DNA nucleotidylexotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000041">
        <property type="entry name" value="DNA nucleotidylexotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000010">
        <property type="entry name" value="DNA polymerase lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000003">
        <property type="entry name" value="DNA polymerase mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019843">
        <property type="entry name" value="DNA_pol-X_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018944">
        <property type="entry name" value="DNA_pol_lambd_fingers_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027292">
        <property type="entry name" value="TdT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001726">
        <property type="entry name" value="TdT/Mu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF21">
        <property type="entry name" value="DNA NUCLEOTIDYLEXOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10391">
        <property type="entry name" value="DNA_pol_lambd_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000817">
        <property type="entry name" value="DNA_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501175">
        <property type="entry name" value="TDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00871">
        <property type="entry name" value="DNAPOLXTDT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81585">
        <property type="entry name" value="PsbU/PolX domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00522">
        <property type="entry name" value="DNA_POLYMERASE_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0780">Terminal addition</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="17">
        <location>
            <begin position="27"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
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        <ligand>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="509" mass="58280" checksum="C03271536884E014" modified="2019-09-18" version="1">MDPLCTASSGPRKKRPRQVGASMASPPHDIKFQNLVLFILEKKMGTTRRNFLMELARRKGFRVENELSDSVTHIVAENNSGSEVLEWLQVQNIRASSQLELLDVSWLIESMGAGKPVEITGKHQLVVRTDYSATPNPGFQKTPPLAVKKISQYACQRKTTLNNYNHIFTDAFEILAENSEFKENEVSYVTFMRAASVLKSLPFTIISMKDTEGIPCLGDKVKCIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRSLSKIMSDKTLKFTKMQKAGFLYYEDLVSCVTRAEAEAVGVLVKEAVWAFLPDAFVTMTGGFRRGKKIGHDVDFLITSPGSAEDEEQLLPKVINLWEKKGLLLYYDLVESTFEKFKLPSRQVDTLDHFQKCFLILKLHHQRVDSSKANQQEGKTWKAIRVDLVMCPYENRAFALLGWTGSRQFERDIRRYATHERKMMLDNHALYDKTKRVFLKAESEEEIFAHLGLDYIEPWERNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6X6</accession>
    <name>A0A4W2C6X6_BOBOX</name>
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            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <begin position="1"/>
            <end position="219"/>
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        </source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="411" mass="47106" checksum="7381C696BF9510AE" modified="2019-09-18" version="1">MLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANVWIIEKAREFQKNICFCFIDYAKAFDCVDHNKLWKILREMGIPDHLTCLLRNLYADQEATVRSGYGTTDWFQIGKGVHQGCILSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLFMKVKVESQKVGLKLNIHKTKIMASGPITSWEIDGETVETVSDFILGGSKITADGDCSHEIKKCLVLGRKVMTNLDSIFKSRDITLPSKVCVVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRFLRVPWTARRSNQSILKEISPGCSLEGLMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRGQQRLRWLDGITDLMDMSLSELRELVMDREAWRAVIHGVAKSQTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7J0</accession>
    <name>A0A4W2C7J0_BOBOX</name>
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        <submittedName>
            <fullName evidence="7">H1.4 linker histone, cluster member</fullName>
        </submittedName>
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    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
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    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H1/H5 family.</text>
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        <property type="gene ID" value="ENSBIXG00000013493.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004495.1">
        <property type="protein sequence ID" value="ENSBIXP00005006382.1"/>
        <property type="gene ID" value="ENSBIXG00005011883.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155501"/>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1" id="KW-0164">Citrullination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="36"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
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        </location>
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            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
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            <end position="140"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
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            <end position="160"/>
        </location>
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            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
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    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007546.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="219" mass="21889" checksum="ADD676C073EF660B" modified="2019-09-18" version="1">MSETAPAAPAAPAPAEKTPVKKKARKAAGAAKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAATGEAKPKAKKAGAAKPKKPAGAAKKPKKATGAATPKKSAKKTPKKAKKPAAAAGAKKAKSPKKAKAAKPKKAPKSPAKAKAVKPKAAKPKTAKPKAAKPKKAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C824</accession>
    <name>A0A4W2C824_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hyaluronoglucosaminidase</fullName>
            <ecNumber evidence="5">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CEMIP</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEMIP family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027376895.1">
        <property type="nucleotide sequence ID" value="XM_027521094.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A4W2C824"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001252.1">
        <property type="protein sequence ID" value="ENSBIXP00000007726.1"/>
        <property type="gene ID" value="ENSBIXG00000006445.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018636.1">
        <property type="protein sequence ID" value="ENSBIXP00005034119.1"/>
        <property type="gene ID" value="ENSBIXG00005014994.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879524"/>
    <dbReference type="CTD" id="57214"/>
    <dbReference type="GeneTree" id="ENSGT00940000153636"/>
    <dbReference type="OMA" id="DSCKFAD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd13938">
        <property type="entry name" value="PANDER_like_TMEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052252">
        <property type="entry name" value="CEMIP/CEMIP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055400">
        <property type="entry name" value="CEMIP_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039473">
        <property type="entry name" value="TMEM2_PANDER-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15535:SF15">
        <property type="entry name" value="CELL MIGRATION-INDUCING AND HYALURONAN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15535">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24605">
        <property type="entry name" value="CEMIP_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52031">
        <property type="entry name" value="GG_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610394" description="hyaluronoglucosaminidase" evidence="16">
        <location>
            <begin position="31"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="17">
        <location>
            <begin position="44"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1361" mass="152767" checksum="89935CE8397A6AB6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA02</accession>
    <name>A0A4W2CA02_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">C-type lectin domain family 12 member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CLEC12A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027397092.1">
        <property type="nucleotide sequence ID" value="XM_027541291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CA02"/>
    <dbReference type="STRING" id="30522.A0A4W2CA02"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018093.1">
        <property type="protein sequence ID" value="ENSBIXP00000009678.1"/>
        <property type="gene ID" value="ENSBIXG00000015327.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003577.1">
        <property type="protein sequence ID" value="ENSBIXP00005031814.1"/>
        <property type="gene ID" value="ENSBIXG00005012250.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892450"/>
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    <dbReference type="GeneTree" id="ENSGT00940000162719"/>
    <dbReference type="OMA" id="TWMWHED"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030545">
        <property type="term" value="F:signaling receptor regulator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001199">
        <property type="term" value="P:negative regulation of dendritic cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050777">
        <property type="term" value="P:negative regulation of immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032815">
        <property type="term" value="P:negative regulation of natural killer cell activation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902564">
        <property type="term" value="P:negative regulation of neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042916">
        <property type="entry name" value="CLEC12A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47647:SF2">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 12 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47647">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 12 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="13">
        <location>
            <begin position="133"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="264" mass="30952" checksum="8DA2B18EC49D5F75" modified="2019-09-18" version="1">MSEEVTYADLKFQDSSQTENIQKFDKFEKEDPPVPSHVWRQGALALTLLCLLLLIGLGVFGAICMEKLNKLQNFKEELQRNLSLQLMQNTNSSQKIRNLSIKLEEIATKLCRELYTKNKEHKCKPCPEEWMWHDDKCYLLHAYQRQETWQKSDEICSGYNASLLTIKSKSVLEFIKSLMLSNFWLGLSPRKDNPRYRNLDTVILLHWFTRNTSVINDKMYCGWIHYTTVYYTKCTETKSIICEKLAQSVKIESILMSEVLDGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CB76</accession>
    <name>A0A4W2CB76_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CB76"/>
    <dbReference type="STRING" id="30522.A0A4W2CB76"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017250.1">
        <property type="protein sequence ID" value="ENSBIXP00000009164.1"/>
        <property type="gene ID" value="ENSBIXG00000014870.1"/>
    </dbReference>
    <dbReference type="OMA" id="CLVVVHP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052688">
        <property type="entry name" value="Gamma-glutamyltransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47278">
        <property type="entry name" value="GLUTATHIONE HYDROLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47278:SF1">
        <property type="entry name" value="GLUTATHIONE HYDROLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="489" mass="49483" checksum="C4A047D10A932529" modified="2019-09-18" version="1">MEPEAGPVLYQKLRVWEPSLESEEEEEEISEQLILDASGPHDSSGNKAGRLPGAWAQLVAALLLLAIGFSLAVRQLCSSGASPGALGSGAPPASGHSHRPGVYHHGAIISPAAECSRLGRELFVAGGNIVDAGVGAALCLAVVHPHTTGLGATYWGLFHNSSSGNSTALTSGPAQTLAPGLGLPSALPALHMLHTHFGRLPWPHLLVGPISLAQKGFLVDTSLASALAAQDTKGLCPLLCHANGTPLGPGTQVTNTKLAAVLHKASLAPTPDLSGDALLSLLAEDLGLEGPSVGPRPTLEPALQLPLPQGILFTTPSPSAGPELLELLEASLQSAGPSPAPCPALPQAAAAPRSSVLATVDSSGSVLLLTSSLNSSFGSGHLSPSTGVLLSNLVAESAAGAWACPLIFRDISDDTEVDVLGLVASGTPAAARVMTHALLSHLQAPNPDQQGPTESPRACAQGTLLQVAAHTEHAHVSSVPSGCCPFQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCD4</accession>
    <name>A0A4W2CCD4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCD4"/>
    <dbReference type="STRING" id="30522.A0A4W2CCD4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017956.1">
        <property type="protein sequence ID" value="ENSBIXP00000009596.1"/>
        <property type="gene ID" value="ENSBIXG00000015251.1"/>
    </dbReference>
    <dbReference type="OMA" id="WINGMLY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF21">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="82" mass="9633" checksum="18905FA37C1EE7BA" modified="2019-09-18" version="1">TEKLISNFLKRYSDHSQYWIGLRRKPGQTWKWINGMLYNQDWFKIIGIGECAYLHKIGISSASTHLVRKWICSKPDICTSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFI4</accession>
    <name>A0A4W2CFI4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CFI4"/>
    <dbReference type="STRING" id="30522.A0A4W2CFI4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021260.1">
        <property type="protein sequence ID" value="ENSBIXP00000011733.1"/>
        <property type="gene ID" value="ENSBIXG00000016931.1"/>
    </dbReference>
    <dbReference type="OMA" id="YQCHSCG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000620">
        <property type="entry name" value="Zinc finger protein 263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002004">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000754">
        <property type="entry name" value="Zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001396">
        <property type="entry name" value="Zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001745">
        <property type="entry name" value="Zinc finger protein 658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001011">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="624"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="655" mass="75347" checksum="70099123E8EBC6F6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CH66</accession>
    <name>A0A4W2CH66_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 579</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CH66"/>
    <dbReference type="STRING" id="30522.A0A4W2CH66"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045585.1">
        <property type="protein sequence ID" value="ENSBIXP00000012425.1"/>
        <property type="gene ID" value="ENSBIXG00000017504.1"/>
    </dbReference>
    <dbReference type="OMA" id="SPRYTHW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000111">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000344">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF14">
        <property type="entry name" value="ZINC FINGER PROTEIN 579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="121"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="451"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="544" mass="58058" checksum="5D82EE7A17857B2C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHK5</accession>
    <name>A0A4W2CHK5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
            <shortName evidence="16">CNP</shortName>
            <shortName evidence="16">CNPase</shortName>
            <ecNumber evidence="6 16">3.1.4.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CNP</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates. May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>a nucleoside 2',3'-cyclic phosphate + H2O = a nucleoside 2'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14489"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:66954"/>
            <dbReference type="ChEBI" id="CHEBI:78552"/>
            <dbReference type="EC" id="3.1.4.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 16">Exists as monomers and homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the 2H phosphoesterase superfamily. CNPase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.37" evidence="6 16"/>
    <dbReference type="RefSeq" id="XP_027372652.1">
        <property type="nucleotide sequence ID" value="XM_027516851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHK5"/>
    <dbReference type="STRING" id="30522.A0A4W2CHK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044992.1">
        <property type="protein sequence ID" value="ENSBIXP00000011210.1"/>
        <property type="gene ID" value="ENSBIXG00000002564.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056630.1">
        <property type="protein sequence ID" value="ENSBIXP00005046788.1"/>
        <property type="gene ID" value="ENSBIXG00005031498.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877087"/>
    <dbReference type="CTD" id="1267"/>
    <dbReference type="GeneTree" id="ENSGT00510000048410"/>
    <dbReference type="OMA" id="DAYKINP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009214">
        <property type="term" value="P:cyclic nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048709">
        <property type="term" value="P:oligodendrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000795">
        <property type="entry name" value="Tetratricopeptide repeat protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1740.10:FF:000001">
        <property type="entry name" value="Tetratricopeptide repeat protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1740.10">
        <property type="entry name" value="2',3'-cyclic nucleotide 3'-phosphodiesterase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CNPase"/>
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    </dbReference>
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        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10156:SF0">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05881">
        <property type="entry name" value="CNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000970">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Cyclic nucleotide phosphodiesterase catalytic" evidence="19">
        <location>
            <begin position="185"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="329"/>
        </location>
    </feature>
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        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000610"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008662"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011781"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012317"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00014478"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
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        <source>
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            <dbReference type="PIRSR" id="PIRSR000970-2"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF05881"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011210.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="420" mass="47305" checksum="2474115751AC9E54" modified="2019-09-18" version="1">MSRGFSRKSQTFLPKVFFRKMSSSGAKDKPELQFPFLQDEETVATLQECKTLFILRGLPGSGKSTLARFIVDKYRDGTKMVSADSYKITPGARGSFSEEYKQLDEDLAACCRRDFRVLVLDDTNHERERLEQLFELADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSAALWKTGQTFLEELGNHKAFKKELRHFVSGDEPREKIELVTYFGKRPPGVLHCTTKFCDYGKAAGAEEYAQQDVVKKSYCKAFTLTISALFVTPKTTGARVELSEQQLALWPNDVDKLSPSDNLPRGSRAHITLGCAGDVEAVQTGIDLLEIVRQEKGGSRGEEVGELSRGKLYSLGSGRWMLSLAKKMEVRAIFTGYYGKGKAVPIRSGRKGGSFQSCTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIL7</accession>
    <name>A0A4W2CIL7_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Coiled-coil domain containing 97</fullName>
        </submittedName>
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    <gene>
        <name evidence="3" type="primary">CCDC97</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSBIXG00000016825.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005036016.1"/>
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000015495"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040233">
        <property type="entry name" value="CCD97-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018613">
        <property type="entry name" value="Ccdc97-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31840">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31840:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09747">
        <property type="entry name" value="CCD97-like_C"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCD97-like C-terminal" evidence="2">
        <location>
            <begin position="154"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="219"/>
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            <begin position="290"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09747"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="341" mass="39051" checksum="6E3DE1939B0FAC73" modified="2019-09-18" version="1">MEAVATAAREPGEGHTEPSPGHWGELSWTPVLPRPQDKAEAAEGTPRALDADPPEVEDPAVSAMLHAVAASRLPVCSQQQGEPDLTEPEKVAILGQLYHEKPLVFLERFRTGLREEHLPCFGHLRGDHRADFYCAEVARQGTTRPRTLRTRLRNRRYAALRELIQGGEYFSDEQMRFRAPLLYEQYIGQYLTQEELSARTPAHRPPKPGPPGTPTCPLSDLLLQSYQERELQQRLLQQQEEEEACLEEEDEEEDEDEEDQRPGKDSEAWVPDSEERLILREEFTSRMHQRFLDGKDGDFDYSTVDDNPDFDNLDIVARDEEERYFDEEEPEDAPSPELDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ04</accession>
    <name>A0A4W2CJ04_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neurotrophin-4</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJ04"/>
    <dbReference type="STRING" id="30522.A0A4W2CJ04"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045890.1">
        <property type="protein sequence ID" value="ENSBIXP00000013087.1"/>
        <property type="gene ID" value="ENSBIXG00000018056.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:synaptic vesicle"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:growth factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005163">
        <property type="term" value="F:nerve growth factor receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021675">
        <property type="term" value="P:nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038180">
        <property type="term" value="P:nerve growth factor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Brain-derived neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020408">
        <property type="entry name" value="Nerve_growth_factor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002072">
        <property type="entry name" value="Nerve_growth_factor-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019846">
        <property type="entry name" value="Nerve_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020432">
        <property type="entry name" value="Neurotrophin-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11589">
        <property type="entry name" value="NERVE GROWTH FACTOR NGF -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11589:SF8">
        <property type="entry name" value="NEUROTROPHIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00243">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01915">
        <property type="entry name" value="NEUROTROPHN4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00268">
        <property type="entry name" value="NGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00140">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00248">
        <property type="entry name" value="NGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50270">
        <property type="entry name" value="NGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Nerve growth factor-related" evidence="7">
        <location>
            <begin position="185"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="306" mass="31725" checksum="870B7450C8854E1D" modified="2019-09-18" version="1">MGRGGCNLFQPPSSSASPLPSPLLPPSAFHPGAITWAEQGLLGGAAALTLGPLHFQRNTEKRSVLGFRPPPPPASLTCLFPFPLGGCPLPLLRCQEGGGHLCPQQGPPGAWGPPAPELGGGGGADRSSNHPLLLMGPLVPRVALSRGTPTGPPLLFLLESGAFGEPVGSPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKAASAGEGGPGGGGGGCRGVDRRHWVSECKAKQSYVRALTTDAQGRVGWRWIRIDTACVCTLLSRTGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLD0</accession>
    <name>A0A4W2CLD0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase PPP1R11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Atypical E3 ubiquitin-protein ligase which ubiquitinates TLR2 at 'Lys-754' leading to its degradation by the proteasome. Plays a role in regulating inflammatory cytokine release and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2. Inhibitor of protein phosphatase 1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with TLR2 and UBE2D2.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLD0"/>
    <dbReference type="STRING" id="30522.A0A4W2CLD0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002471.1">
        <property type="protein sequence ID" value="ENSBIXP00000007112.1"/>
        <property type="gene ID" value="ENSBIXG00000013108.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="147"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="203" mass="22440" checksum="40B7712C0E0CE4ED" modified="2019-09-18" version="1">MDSATGRLNRVRYPVFRNRPSSNLHRGFLLNLCFARPCSHCSPRLLLPYKSDASSAKVVSPPCGACALSHYGGTNPATQRFDAFSTRKGKGGPQNASHPEENRSLTIKLRKRKPEKKVEWTSDTVDNEHMGRRSSKCCCIYEKPRAFGESSTESDDEEEEGCGHTHCVRGHRKGRRHATPGPSPTSPPQPPDPSQPPPGPMQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CME3</accession>
    <name>A0A4W2CME3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CME3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001780.1">
        <property type="protein sequence ID" value="ENSBIXP00000007479.1"/>
        <property type="gene ID" value="ENSBIXG00000013420.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000007">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000061">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000117">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000148">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000310">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000431">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000445">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000473">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000824">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000006">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056986">
        <property type="entry name" value="JAG1_1/2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026219">
        <property type="entry name" value="Jagged/Serrate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01414">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23575">
        <property type="entry name" value="JAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02059">
        <property type="entry name" value="JAGGEDFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 16" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12 14 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 14 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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        <property type="term" value="P:regulation of dendrite development"/>
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        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
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        <property type="term" value="P:single fertilization"/>
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        <property type="entry name" value="adapter molecule crk isoform X1"/>
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        <property type="entry name" value="crk-like protein isoform X1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
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        <property type="entry name" value="CRK-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
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    <dbReference type="PROSITE" id="PS50002">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="14"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="123"/>
            <end position="183"/>
        </location>
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    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="234"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="184"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00059037"/>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="302" mass="33244" checksum="51DA5C030BB058DD" modified="2019-09-18" version="1">MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGVFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPVGSVSAPSLPAAEETLEYVRTLYDFPGNDAEDLPFKKGEILVIVEKPEEQWWSARNKDGRVGMIPVPYVEKLVRASAQGKPGNRNSSSYGVPEPAHAYAQPQTTAPPPAAGAPGAAVSPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIIDPQNPDESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ59</accession>
    <name>A0A4W2CQ59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Epithelial splicing regulatory protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RNA-binding motif protein 35A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RNA-binding protein 35A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ESRP1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESRP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027417315.1">
        <property type="nucleotide sequence ID" value="XM_027561514.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSBIXT00000026658.1">
        <property type="protein sequence ID" value="ENSBIXP00000015305.1"/>
        <property type="gene ID" value="ENSBIXG00000003562.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009030.1">
        <property type="protein sequence ID" value="ENSBIXP00005004086.1"/>
        <property type="gene ID" value="ENSBIXG00005010129.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904413"/>
    <dbReference type="CTD" id="54845"/>
    <dbReference type="GeneTree" id="ENSGT00940000159511"/>
    <dbReference type="OMA" id="MISEPYX"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12736">
        <property type="entry name" value="RRM1_ESRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12739">
        <property type="entry name" value="RRM2_ESRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12742">
        <property type="entry name" value="RRM3_ESRP1_ESRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000041">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000070">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000023">
        <property type="entry name" value="epithelial splicing regulatory protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000056">
        <property type="entry name" value="epithelial splicing regulatory protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034427">
        <property type="entry name" value="ESRP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="225"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="326"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="681" mass="75375" checksum="58373189B1BFB3EC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRH6</accession>
    <name>A0A4W2CRH6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4 14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PYCR1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:14107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:14111"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRH6"/>
    <dbReference type="STRING" id="30522.A0A4W2CRH6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043840.1">
        <property type="protein sequence ID" value="ENSBIXP00000008903.1"/>
        <property type="gene ID" value="ENSBIXG00000006090.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000064">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000003">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF6">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 14" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="16">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="17">
        <location>
            <begin position="164"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="434" mass="45392" checksum="CAFF5D2AA36C5DCC" modified="2019-09-18" version="1">MSVGFIGAGQLAFALAKGFTAAGVVAAHKIMASSPDMDLATVSALRKMGVNLTHHNKETVQHSDVLFLAVKPHIIPFILDEIAANIEARHIVVSCAAGVTISSIEKKLTAFQPAPKVIRCMTNTPVVVREGATVYATGTHAQVEDGRLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQHPGQLKDNVCSPGGATIHALHVLESGGFRSLLIKAVEASCTRTRWASATAHPLCHWLNRWLSSTHLAVGRARGALGLQCTASLTIPHHAVRRRCLPGPGCAPHCFITCGLLTGVVIQAREAGQEEISVGLMIPPSSWPCGESERCAGGTGTTSVRSVKAERAGDRHGVASWLRVPVASSGLQDGQDCFSASYLPSSPSPQASLPLSDSDART</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRL9</accession>
    <name>A0A4W2CRL9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ZFP3 zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZFP3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRL9"/>
    <dbReference type="SMR" id="A0A4W2CRL9"/>
    <dbReference type="STRING" id="30522.A0A4W2CRL9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016867.1">
        <property type="protein sequence ID" value="ENSBIXP00000008949.1"/>
        <property type="gene ID" value="ENSBIXG00000014665.1"/>
    </dbReference>
    <dbReference type="OMA" id="YHECSEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000111">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001738">
        <property type="entry name" value="ZFP3 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001121">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001542">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="507" mass="58337" checksum="75AFDFEBECBC74DE" modified="2019-09-18" version="1">MGTENKEVIPKEEISEESQPHGAFLEKMEEKLPKVVCQGHEFGAVCEEDILEGHSRESTEKILGQESSEERDFASELIIFKKSPSSEKDQEKDESERICRLSSNLLTRQGDTTLEAVSTFATSGQNFIENLGPNKTHRSSVGEKPHTCKECGKAFNQNSHLIQHMRVHSGEKPFECKECGKTFGTNSSLRRHLRIHAGEKPFACNECGKAFIQSSHLIHHHRIHTGERPYKCEECGKAFSQNSALILHQRIHTGEKPYECNECGKTFRVSSQLIQHQRIHTEERYHECNECGKAFKHSSGLIRHQKIHTGEKPYLCNECGKGFGQSSELIRHQRIHTGDKPYECNECGKTFGQNSEIIRHIRIHTGEKPYVCKECGKAFRGNSELLRHERIHTGEKPYECFECGKAFRRTSHLIVHQRIHTGEKPHQCNECARTFWDNSELLLHQKIHIGEKPYECNECEKTFSQHSQLIIHQRIHTGEKPYECQECQKTFSRSSHLLRHQSVHCME</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWC4</accession>
    <name>A0A4W2CWC4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stabilizer of axonemal microtubules 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SAXO1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWC4"/>
    <dbReference type="SMR" id="A0A4W2CWC4"/>
    <dbReference type="STRING" id="30522.A0A4W2CWC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030354.1">
        <property type="protein sequence ID" value="ENSBIXP00000017712.1"/>
        <property type="gene ID" value="ENSBIXG00000004143.1"/>
    </dbReference>
    <dbReference type="OMA" id="KQPVPME"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF9">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05217">
        <property type="entry name" value="SAXO1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="477" mass="54593" checksum="1DEBF8C960D207A2" modified="2019-09-18" version="1">MAPTKGKCVCELCSCGRHHCPHLPTKIYDKTEKPCLLSEYTENYPVYHSYLPRESFKPKMDYQRACTPMEGLTTSRRDFGPHKVLPVKIHQPNPFVPSEENMDLQTTYKQDYNPYPLCRVDPFKPRDSKYPCGDKMESLPTYKADYLPWNQPRRELLRPPHHYRPASTKFDSRTTQQDDYSMKGLVNTRSCKPPAVPKLCNVPLEDLTNYKMSYVAHPLEKRFVHESEKFRPCEIPFESLTTHKESYRGLMGEPAKSLKPPARPYGLDTPFSNTTEFRDKYQAWPTPQVFSKPPSMYVPPEEKMDLLTTVQTHYTYPKGAPAESCRPALSVKKGGRFEGSTTTKEDYKQWASTRTEPAKPIPQLNLPTEPLDCLTTARAHYVPHLPMMTKSCKPVWSGPQGNIPVEGQTTYTISFTPKEMSRCLASYPEPPGYIFEEIDALGHRIYRPVSQTGSRRSSRFSVGDSENPNQQELTVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWP9</accession>
    <name>A0A4W2CWP9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Kinesin-like protein KIF16B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">KIF16B</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000019736.1">
        <property type="protein sequence ID" value="ENSBIXP00000010738.1"/>
        <property type="gene ID" value="ENSBIXG00000007703.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06874">
        <property type="entry name" value="PX_KIF16B_SNX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000005">
        <property type="entry name" value="Kinesin family member 16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000022">
        <property type="entry name" value="Kinesin family member 16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000021">
        <property type="entry name" value="kinesin-like protein KIF16B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF8">
        <property type="entry name" value="KINESIN FAMILY MEMBER 16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kinesin motor" evidence="20">
        <location>
            <begin position="3"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="21">
        <location>
            <begin position="1171"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="631"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="872"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1076"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="373"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="954"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="872"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1306" mass="149561" checksum="5FD6578F551AC7D9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CY28</accession>
    <name>A0A4W2CY28_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PTPRT</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000022196.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005000433.1">
        <property type="protein sequence ID" value="ENSBIXP00005032871.1"/>
        <property type="gene ID" value="ENSBIXG00005013934.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155326"/>
    <dbReference type="OMA" id="MPEYDTE"/>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0051393">
        <property type="term" value="F:alpha-actinin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045294">
        <property type="term" value="F:alpha-catenin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
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        <property type="term" value="F:delta-catenin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045295">
        <property type="term" value="F:gamma-catenin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:protein phosphatase binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:STAT family protein binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:thiolester hydrolase activity"/>
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        <property type="term" value="F:transmembrane receptor protein tyrosine phosphatase activity"/>
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        <property type="term" value="P:cellular response to interleukin-6"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of cell migration"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of receptor signaling pathway via STAT"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd14630">
        <property type="entry name" value="R-PTPc-T-1"/>
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    </dbReference>
    <dbReference type="CDD" id="cd14634">
        <property type="entry name" value="R-PTPc-T-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000006">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000152">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000009">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000025">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF12">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="MAM" evidence="20">
        <location>
            <begin position="7"/>
            <end position="168"/>
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    <sequence length="1456" mass="163202" checksum="773448F0A6A0B26A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZ06</accession>
    <name>A0A4W2CZ06_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8">Myosin light chain kinase, smooth muscle</fullName>
            <ecNumber evidence="7">2.7.11.18</ecNumber>
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            <fullName evidence="28">Kinase-related protein</fullName>
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        <alternativeName>
            <fullName evidence="26">Telokin</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="34 35" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <cofactor evidence="1">
            <name>Ca(2+)</name>
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            <name>Mg(2+)</name>
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    <comment type="subunit">
        <text evidence="27">All isoforms including Telokin bind calmodulin. Interacts with SVIL. Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2.</text>
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        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
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        <subcellularLocation>
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            <location evidence="4">Cytoskeleton</location>
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        <text evidence="6">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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    <keyword evidence="18 29" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="15 29" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
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    <feature type="domain" description="Ig-like" evidence="32">
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            <begin position="306"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="999"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1434"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1659"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="2014"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="1029"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1306"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="30">
        <location>
            <begin position="1674"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="2017"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="1740"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00030959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00062188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00078563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2163" mass="236198" checksum="8116228BEB75D684" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1Y2</accession>
    <name>A0A4W2D1Y2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SUMO1 activating enzyme subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SAE1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1Y2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022706.1">
        <property type="protein sequence ID" value="ENSBIXP00000012655.1"/>
        <property type="gene ID" value="ENSBIXG00000017728.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036110.1">
        <property type="protein sequence ID" value="ENSBIXP00005041773.1"/>
        <property type="gene ID" value="ENSBIXG00005024933.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075007"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:SUMO activating enzyme complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:SUMO activating enzyme activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Aos1_SUMO"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="2">
        <location>
            <begin position="19"/>
            <end position="256"/>
        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="265" mass="29278" checksum="11599E2E5B4143C9" modified="2019-09-18" version="1">MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASQVLLVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTENIEKKPESFFTQFDAKVVFCSVKEALEVDWSSDKAKAALKRTTPDYFLLQVLLKFRTDKGRDPSSDTFGEDSELLLQIRNDVLDALGVNPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3X2</accession>
    <name>A0A4W2D3X2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3X2"/>
    <dbReference type="SMR" id="A0A4W2D3X2"/>
    <dbReference type="STRING" id="30522.A0A4W2D3X2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023790.1">
        <property type="protein sequence ID" value="ENSBIXP00000013378.1"/>
        <property type="gene ID" value="ENSBIXG00000018315.1"/>
    </dbReference>
    <dbReference type="OMA" id="MPECEMT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000878">
        <property type="entry name" value="Zinc finger protein 544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000416">
        <property type="entry name" value="zinc finger protein 879 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="299" mass="33633" checksum="39A1CA4868AD607C" modified="2019-09-18" version="1">MREGPLRPGTGPQGKELPGKVNPDHHGLGTDDCLPSRTAQQRVPPGDILYERESRGLVKGPLTHEGNFPCKCGECGKAFHRRSQLTTHQLIHTGEKPYECSECGKGFCDSTRLIKHSIIHTGEKPYNCLECGKAFYRKSYLKEHQRIHTGEKPYVCTECGRAFVYCSTFILHKRAHTGEKPYGCKECGKAFSSRSSLSRHFTIHSAEKPYKCTECGKAFNRRAYLSSHQRIHSGEKPYTCVECRKTFWWSSSLVRHSIIHTGEKPCDSSLTQRQRVHTGGSPVSVTDVGQPRTLTSFHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D424</accession>
    <name>A0A4W2D424_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D424"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034770.1">
        <property type="protein sequence ID" value="ENSBIXP00000020594.1"/>
        <property type="gene ID" value="ENSBIXG00000004772.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="408" mass="47076" checksum="764476A20FB663D1" modified="2019-09-18" version="1">MLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANILWFIEKAREFQKNLYFCFIDYAKAFDCVDHNQLWKILKEMGIPYHLTCLLRNLYAGQEATVKTGHGTTDWFQIGKGVRQGCILSHCLFNFYAEYIMINAALEEAQAGIKIAGRNINNLRYADDTTLTAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVQTVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNVDSIFKSRDITLPTKVRLVKAMVFPVGMYGCESWTVKKAEHRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRVDSLEKTLMLGGRRRGRQRMRWLDGITDSMDVSLSELWELVMDREAWHAAIHGVSKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D618</accession>
    <name>A0A4W2D618_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 239</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D618"/>
    <dbReference type="STRING" id="30522.A0A4W2D618"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033393.1">
        <property type="protein sequence ID" value="ENSBIXP00000019682.1"/>
        <property type="gene ID" value="ENSBIXG00000023233.1"/>
    </dbReference>
    <dbReference type="OMA" id="IHREVHA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001195">
        <property type="entry name" value="Zinc finger protein 239"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002357">
        <property type="entry name" value="Zinc finger protein 782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF143">
        <property type="entry name" value="ENDOTHELIAL ZINC FINGER PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="404" mass="45817" checksum="118D07A30C55709B" modified="2019-09-18" version="1">MESEDYSSLKVPIQMATQDSSFCKKEPQDPQESKSLFVPEESTERKLTGAKSPPIEHCSENLQDKLVADVKEVVLPLLKGETVCHTGQLKESLEPFDYNHKDVCGWKSRVASHHYQRAHTEEKPCSHQDCGKTCTASPGGHPGEKIRPSQKVFKCSQCGRDFRERSDLVLHQRDHTEEKPYRCDQCGKGFTRSSSLLIHREVHADEKPYKCDKCGKGFTRSSSLLIHHSVHTGEKPYKCDKCGKGFTQSSKLHIHQRVHTGEKPYECGECGMSFSQRSNLHIHQRIHTGERPYKCGECGKGFSQSSNLHIHRCSHTGEKPYQCYDCGKGFSQSSDLRIHLRVHTGEKPYHCGKCGKGFSQSSKLLIHQRVHTGEKPYECNKCGKGFSQSSNLHIHQRVHRKDPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D688</accession>
    <name>A0A4W2D688_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Embryonal Fyn-associated substrate</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EFS</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSBIXT00000033484.1">
        <property type="protein sequence ID" value="ENSBIXP00000019757.1"/>
        <property type="gene ID" value="ENSBIXG00000004591.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005037721.1">
        <property type="protein sequence ID" value="ENSBIXP00005023078.1"/>
        <property type="gene ID" value="ENSBIXG00005025931.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183008"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="P:cell migration"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FAT-like_EFS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Breast cancer anti-estrogen resistance 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAS_C"/>
    </dbReference>
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        <property type="entry name" value="CAS_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654">
        <property type="entry name" value="CAS SCAFFOLDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654:SF14">
        <property type="entry name" value="EMBRYONAL FYN-ASSOCIATED SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12026">
        <property type="entry name" value="CAS_C"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAS family C-terminal" evidence="3">
        <location>
            <begin position="279"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="468" mass="48677" checksum="7CB229A6B21B0507" modified="2019-09-18" version="1">MAIATSLYVVPPPARLCPTSRPPTGPCPPSPDPIYKVPRGGGTQLAAPGAALEVYDVPPTALRVPSNGPYDSPASFARPLAQVAPQSLGEDEAPYDVPLAPKSPSDLELDLEWEGGREPGPPLYAAPSNLKRASALLNLYEAPEELLADGEGGGADEGIYDVPLLGPETPPSPEPLGASASSDLDTLALLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPAPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREVEDHPAGHHNEYEGIPVAEEYDYVHLKGMDKVQEARPPDKASPGDPEQLEREPPEQQEALSPGESLVLPTGDLQLLHFYAGQCQGHYSTLQAAVAALMSSTQAKQPPHLFVPHGKRALVAAHRLVFVGDTLGRLAASAPLRAQVGAAGTALGQALRATVLAIKGAALGYPSSPAAEEMAQCVADLAGQALQFTTLLTSLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7H1</accession>
    <name>A0A4W2D7H1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">To the propeptide regions of cysteine proteases.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7H1"/>
    <dbReference type="SMR" id="A0A4W2D7H1"/>
    <dbReference type="STRING" id="30522.A0A4W2D7H1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036693.1">
        <property type="protein sequence ID" value="ENSBIXP00000021837.1"/>
        <property type="gene ID" value="ENSBIXG00000005049.1"/>
    </dbReference>
    <dbReference type="OMA" id="EGETCCC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cathepsin L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000006">
        <property type="entry name" value="Cathepsin S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021313649" evidence="12">
        <location>
            <begin position="21"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="120"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="334" mass="37559" checksum="31EE3E0C2F31E25F" modified="2019-09-18" version="1">MPINRMWGLTVLLLPVVSFALYPEEILDTQWELWKKTYRKQYNSKGDEISRRLIWEKNLKHISIHNLEASLGVHTYELAMNHLGDMTSEEVVQKMTGLKVPASRSRSNDTLYIPDWEGRAPDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGCGGGYMTNAFQYVQKNRGIDSEDAYPYVGQDENCMYNPTGKAAKCRGYREIPEGNEKALKRAVARVGPISVAIDASLTSFQFYRKGVYYDENCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFPKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8W7</accession>
    <name>A0A4W2D8W7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Fibrinogen C domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">FIBCD1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Homotetramer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8W7"/>
    <dbReference type="STRING" id="30522.A0A4W2D8W7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026518.1">
        <property type="protein sequence ID" value="ENSBIXP00000015201.1"/>
        <property type="gene ID" value="ENSBIXG00000019754.1"/>
    </dbReference>
    <dbReference type="OMA" id="MVNERWK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF45">
        <property type="entry name" value="FIBRINOGEN C DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="16">
        <location>
            <begin position="235"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="461" mass="50498" checksum="046CC37B7EFCC560" modified="2019-09-18" version="1">MVNDRWKTMGGASQLEDRPRDKPQRPSCSYVLCTVLLSLAVLLAVAVTGAVLLLNHARVPGTAPPPVVSTGPAGANSALVTVDRADSSRLSILIDPRCPDLADGFARLEGAQASVLQALSQRQAEPRLPGEQEQELLDTLADQLPRLLARASELQAECSGLRKGHGALGQGLSALQSEQGRLIQLLSESQGHMAHLVNSVSDVLDALQRDRGLARPHVKADLQRAPARGSRPRGCANGSRPRDCLDVLLSGQQEDGVYSVFPTHDPAGFQVYCDMRTDGGGWTVFQRREDGSVNFFRGWEAYRDGFGKLTGEHWLGLKRIHALTTQAAYELHVDLEDFDNGTAYAHYGSFGVGLFSVDPEEDGYPLTVADYSGTAGDSLLKHSGMKFTTKDRDSDHSENNCAAFYRGAWWYRNCHTSNLNGQYLRGAHASYADGVEWSSWTGWQYSLKFSEMKIRPVREDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8Y9</accession>
    <name>A0A4W2D8Y9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-binding cassette sub-family F member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8Y9"/>
    <dbReference type="STRING" id="30522.A0A4W2D8Y9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035006.1">
        <property type="protein sequence ID" value="ENSBIXP00000020639.1"/>
        <property type="gene ID" value="ENSBIXG00000023929.1"/>
    </dbReference>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000688">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058770">
        <property type="entry name" value="PWI_ABCF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26051">
        <property type="entry name" value="PWI_ABCF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="178"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="492"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="709" mass="79796" checksum="0D87674A34858340" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9R3</accession>
    <name>A0A4W2D9R3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GJB3</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9R3"/>
    <dbReference type="STRING" id="30522.A0A4W2D9R3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038024.1">
        <property type="protein sequence ID" value="ENSBIXP00000022748.1"/>
        <property type="gene ID" value="ENSBIXG00000025536.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038292.1">
        <property type="protein sequence ID" value="ENSBIXP00005023467.1"/>
        <property type="gene ID" value="ENSBIXG00005006016.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234513"/>
    <dbReference type="OMA" id="HFFPISN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="248" mass="28834" checksum="EB18AF16382DD8D1" modified="2019-09-18" version="1">MISTCENALASQTGRRRRDTAERLHCSQDLNSKWKINALQPPQSEVKVNIWEIKPPDFSYKLYTSLRLAEKPSRTIMQEERRKIPDFPGKMVHLPSLRNPPKKAVSPKFITTFPRLDSHKAKLMFVESGKYPNGVYLNPKPHDFRQNECNLPNFVTTYEKDPFGLKFKSQHLSTVSGCPLLKNGQQNSTERFSTYKPRECAWDSKLILPKAPWPVKSASYTRHRRRRGAYSAFMDRVEEKFNKTCKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAX6</accession>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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        <text evidence="5">Belongs to the SPATA31 family.</text>
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        <property type="component" value="Chromosome 8"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR027970">
        <property type="entry name" value="SPATA31-like"/>
    </dbReference>
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        <property type="entry name" value="ACROSOME-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN SPATA31F1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15371">
        <property type="entry name" value="DUF4599"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAM75"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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        <location>
            <begin position="51"/>
            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="417"/>
            <end position="445"/>
        </location>
    </feature>
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        <location>
            <begin position="475"/>
            <end position="617"/>
        </location>
    </feature>
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        <location>
            <begin position="542"/>
            <end position="567"/>
        </location>
    </feature>
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            <begin position="917"/>
            <end position="952"/>
        </location>
    </feature>
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        <location>
            <begin position="1014"/>
            <end position="1087"/>
        </location>
    </feature>
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            <end position="1137"/>
        </location>
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        <location>
            <begin position="1224"/>
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        </location>
    </feature>
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        <location>
            <begin position="1248"/>
            <end position="1277"/>
        </location>
    </feature>
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        <location>
            <begin position="551"/>
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        </location>
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        <location>
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            <end position="1026"/>
        </location>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="1256"/>
            <end position="1270"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF14650"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021405.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1334" mass="148336" checksum="09E3E7D74CAF8505" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DB73</accession>
    <name>A0A4W2DB73_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Taste receptor type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113893637</name>
    </gene>
    <organism evidence="15 16">
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
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        <text evidence="2 12">Belongs to the G-protein coupled receptor T2R family.</text>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:bitter taste receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Taste receptor type 2 member 7"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TAS2R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394:SF63">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2 MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05296">
        <property type="entry name" value="TAS2R"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
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    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
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    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="129"/>
            <end position="153"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="199"/>
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            <begin position="230"/>
            <end position="253"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="259"/>
            <end position="282"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007376"/>
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            <dbReference type="ARBA" id="ARBA00022606"/>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Ensembl" id="ENSBIXP00000023267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="309" mass="35107" checksum="89DF9B663638F5B9" modified="2019-09-18" version="1">MLSIVEGLLLFVAVNESVLGVLGNGFIGLVNCINCVKNKKISTLSLILTGLASSRFCLIWIITTDAYVRVFSPDMYLSGNLSQYIAYLWIIMNQSSVWFTTSLSIFYFLKIANFSHCIFLWLKGHITEILLLLMGCLPISWLFTFPNITMPFINNIMKNRSTTGLVTMQKSEYFINQILFNLGTFLVFVLCLITCFLIITSLWRHNRRMQLNATGFRDPSTEAHIKAMKILVSFIILFILYFVGTAIQILSVTVPENKLLFIFGMTTTILYPCGHSFILILGNSKLNQASLRVLKLLKSSKQNFSLYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDY1</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="30522.A0A4W2DDY1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 24"/>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 24"/>
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    </dbReference>
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        <location>
            <begin position="538"/>
            <end position="574"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="574" mass="62098" checksum="93C0F7109A627BE2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DED4</accession>
    <name>A0A4W2DED4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-fold modifier 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with UBA5. Interacts with UFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UFM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DED4"/>
    <dbReference type="STRING" id="30522.A0A4W2DED4"/>
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        <property type="gene ID" value="ENSBIXG00000025366.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01766">
        <property type="entry name" value="Ubl_UFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000044">
        <property type="entry name" value="Ubiquitin-fold modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005375">
        <property type="entry name" value="UFM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825:SF5">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03671">
        <property type="entry name" value="Ufm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="107" mass="11191" checksum="C3C925A5EDDFA9C2" modified="2019-09-18" version="1">MSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIILEIVGSVFHCCLAAHTGSSSGVNGSTICVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJN3</accession>
    <name>A0A4W2DJN3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Janus kinase and microtubule interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">JAKMIP2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the JAKMIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401847.1">
        <property type="nucleotide sequence ID" value="XM_027546046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJN3"/>
    <dbReference type="SMR" id="A0A4W2DJN3"/>
    <dbReference type="STRING" id="30522.A0A4W2DJN3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040708.1">
        <property type="protein sequence ID" value="ENSBIXP00000024515.1"/>
        <property type="gene ID" value="ENSBIXG00000026665.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024698.1">
        <property type="protein sequence ID" value="ENSBIXP00005014382.1"/>
        <property type="gene ID" value="ENSBIXG00005003009.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895197"/>
    <dbReference type="CTD" id="9832"/>
    <dbReference type="GeneTree" id="ENSGT00940000153713"/>
    <dbReference type="OMA" id="RNLELEX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024836">
        <property type="entry name" value="JAKMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031994">
        <property type="entry name" value="JAKMIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935:SF7">
        <property type="entry name" value="JANUS KINASE AND MICROTUBULE-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16034">
        <property type="entry name" value="JAKMIP_CC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Janus kinase and microtubule-interacting protein C-terminal" evidence="5">
        <location>
            <begin position="409"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="281"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="471"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="667"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="820" mass="96212" checksum="99D2E1A12350BAD0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DME2</accession>
    <name>A0A4W2DME2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
            <ecNumber evidence="13">4.4.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cysteine desulfhydrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Cysteine-protein sulfhydrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Gamma-cystathionase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Homocysteine desulfhydrase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CTH</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L,L-cystathionine + H2O = 2-oxobutanoate + L-cysteine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14005"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="4.4.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14006"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-cysteine + H2O = hydrogen sulfide + pyruvate + NH4(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24931"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="EC" id="4.4.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:24932"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14501"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.4.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:14502"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24923"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="EC" id="4.4.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-selenocystathionine + H2O = L-selenocysteine + 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:31151"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57843"/>
            <dbReference type="ChEBI" id="CHEBI:62226"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:31152"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homotetramer. Interacts with CALM in a calcium-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EC" id="4.4.1.2" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DME2"/>
    <dbReference type="SMR" id="A0A4W2DME2"/>
    <dbReference type="STRING" id="30522.A0A4W2DME2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042237.1">
        <property type="protein sequence ID" value="ENSBIXP00000025526.1"/>
        <property type="gene ID" value="ENSBIXG00000027605.1"/>
    </dbReference>
    <dbReference type="OMA" id="EHTFVDM"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00202"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047982">
        <property type="term" value="F:homocysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000009">
        <property type="entry name" value="Cystathionine gamma-synthase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 20 21" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="20">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="405" mass="44380" checksum="A90E52B591CD12E0" modified="2019-09-18" version="1">MQEKEASPHGFLPRFQHFATQAIHVGQEPEQWTSQAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYSLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVATEFGLKISFVDCSKPKLLEAAITPETKLVWIETPTNPSLKMIDIEACAHTVHKHGDIILVVDNTFMSAYFQRPLSLGADICMYSATKYMNGHSDVVMGLVSLNSESLHDRLRFLQNSLGAVPSPIDCYLCNRGLKTLQVRMEKHFENGMAVAQFLESNPQVEKVIYPGLPSHPQHELAKRQCTGCPGMVTFYIKGSLQHAETFLQNLKLFTLAESLGGYESLAELPAIMTHASVPKSDREVLGISDTLIRLSVGLEDKQDLLDDLDQALKAANPPNASSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNN6</accession>
    <name>A0A4W2DNN6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNN6"/>
    <dbReference type="STRING" id="30522.A0A4W2DNN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042560.1">
        <property type="protein sequence ID" value="ENSBIXP00000025745.1"/>
        <property type="gene ID" value="ENSBIXG00000027841.1"/>
    </dbReference>
    <dbReference type="OMA" id="KTQSWFE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="Toastrack_DUF4097"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34094">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34094:SF1">
        <property type="entry name" value="PROTEIN FAM185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021312634" description="DUF4097 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4097" evidence="2">
        <location>
            <begin position="121"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="385" mass="41487" checksum="08293EBD4DC84671" modified="2019-09-18" version="1">MFPLSGWGLGCLRLCLLRVWAGEATGRWACGTCQSRLYGSGGSQPEVSRSDPVRGTLKEWALLVSPFGRLRARLPCHLTVRPLDPLTYPDGDRVLVTVSGVEGGARALAGLQVNYDEAQKEVTIVADVIDHQASVEVNAPLKFDLNIQSSGSGCVKVQNIECDNCKIETEQGTSILQSVKSQKLHVQTTGGKVICLGTVYGNIDIHASDKSTVTVDKLQGSSVNISTEDGLLKVKYLYTESSFLSSAAGDITLGSVHGNITLRSKMGNITVDSSSGCLNALAQQGAIDVYVSQLGKVELKVPKGSILVKVPSSLQAYLQLSGKEIDVNSDVHVEEMNEAHRDDGVIITGFLNQTSKHEKWIKADAPKGTVRFRSQSWFQSLKLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP22</accession>
    <name>A0A4W2DP22_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="18">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">PLCD1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Binds phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = 1D-myo-inositol 1-phosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58433"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with TGM2.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="18"/>
    <dbReference type="RefSeq" id="XP_027378534.1">
        <property type="nucleotide sequence ID" value="XM_027522733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DP22"/>
    <dbReference type="SMR" id="A0A4W2DP22"/>
    <dbReference type="GlyCosmos" id="A0A4W2DP22">
        <property type="glycosylation" value="2 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000011455.1">
        <property type="protein sequence ID" value="ENSBIXP00000028059.1"/>
        <property type="gene ID" value="ENSBIXG00000006428.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880505"/>
    <dbReference type="CTD" id="5333"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120548">
        <property type="term" value="F:phosphatidylinositol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16217">
        <property type="entry name" value="EFh_PI-PLCdelta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13363">
        <property type="entry name" value="PH_PLC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08593">
        <property type="entry name" value="PI-PLCc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000071">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000144">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000088">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000098">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000018">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000019">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028391">
        <property type="entry name" value="PLC-delta1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046975">
        <property type="entry name" value="PLC-delta1_EF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF210">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
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        <source>
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    <sequence length="756" mass="86073" checksum="EBA47E8BAEE2D398" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSW5</accession>
    <name>A0A4W2DSW5_BOBOX</name>
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        <recommendedName>
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        <alternativeName>
            <fullName evidence="15">pVHL</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
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        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the VHL family.</text>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:response to hypoxia"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="VHL_alpha_dom_sf"/>
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            <begin position="38"/>
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            <begin position="131"/>
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        <location>
            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="8"/>
            <end position="20"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010057"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059036"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="191" mass="21612" checksum="84857E6260B507FD" modified="2019-09-18" version="1">MPRKAGDAEEAEAGAEEASAEDPGAEREMEAGRLRPVLRSVNTREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTSDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPEDYRRLDIVRSLYEDLEDHPNVQKDLVRLTQEHIESQGEDEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTG1</accession>
    <name>A0A4W2DTG1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Parvin beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PARVB</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the parvin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTG1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005774.1">
        <property type="protein sequence ID" value="ENSBIXP00000029880.1"/>
        <property type="gene ID" value="ENSBIXG00000000937.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071963">
        <property type="term" value="P:establishment or maintenance of cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21336">
        <property type="entry name" value="CH_PARVB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21338">
        <property type="entry name" value="CH_PARVB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000015">
        <property type="entry name" value="Parvin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000011">
        <property type="entry name" value="Parvin, beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028433">
        <property type="entry name" value="Parvin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12114:SF7">
        <property type="entry name" value="BETA-PARVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12114">
        <property type="entry name" value="PARVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039131">
        <property type="entry name" value="Parvin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="50"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="217"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="327" mass="37540" checksum="8F0CEECA1C6A6F8F" modified="2019-09-18" version="1">MSDLQEEGKNAINSPMCPAPVEVHPEDSLLEENEERTVIDPTSRDDPRFKELVKVLIDWINDVLVEERIIVKQLEEDLYDGQVLQKLLEKLADCKLNVAEVTQSEIGQKQKLQTVLEAVQELLRPHSRPLQWTVDSIHGKNLVAILHLLVALAMHFRAPIHLPEHVSVQVVVVRKREGLLHSSHVTEELTTTTEMMMGRFERDAFDTLFDHAPDKLSVVKKSLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEDYFVPLHNFYLTPDSFDQKVHNVSFAFELMLDGGLKKPKARPEDVVNLDLKSTLRVLYNLFTKYKHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTY8</accession>
    <name>A0A4W2DTY8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FA complementation group E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FANCE</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTY8"/>
    <dbReference type="STRING" id="30522.A0A4W2DTY8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005211.1">
        <property type="protein sequence ID" value="ENSBIXP00000030063.1"/>
        <property type="gene ID" value="ENSBIXG00000011860.1"/>
    </dbReference>
    <dbReference type="OMA" id="LRLPWIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07439">
        <property type="entry name" value="FANCE_c-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.480:FF:000002">
        <property type="entry name" value="Fanconi anemia, complementation group E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039685">
        <property type="entry name" value="FANCE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021025">
        <property type="entry name" value="Fanconi_anaemia_gr_E_prot_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32094">
        <property type="entry name" value="FANCONI ANEMIA GROUP E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32094:SF5">
        <property type="entry name" value="FANCONI ANEMIA GROUP E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11510">
        <property type="entry name" value="FA_FANCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fanconi Anaemia group E protein C-terminal" evidence="2">
        <location>
            <begin position="279"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="541" mass="58781" checksum="1144449A5DA5A72A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1C3</accession>
    <name>A0A4W2E1C3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal ATPase PEX6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Peroxisomal biogenesis factor 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PEX6</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Component of the PEX1-PEX6 AAA ATPase complex, a protein dislocase complex that mediates the ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, an essential step for PEX5 recycling. Specifically recognizes PEX5 monoubiquitinated at 'Cys-11', and pulls it out of the peroxisome lumen through the PEX2-PEX10-PEX12 retrotranslocation channel. Extraction by the PEX1-PEX6 AAA ATPase complex is accompanied by unfolding of the TPR repeats and release of bound cargo from PEX5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with PEX1; forming the PEX1-PEX6 AAA ATPase complex, which is composed of a heterohexamer formed by a trimer of PEX1-PEX6 dimers. Interacts with PEX26; interaction is direct and promotes recruitment to peroxisomal membranes. Interacts with ZFAND6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
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        <property type="entry name" value="DPBB_PEX6"/>
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    </dbReference>
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        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEROXISOMAL ATPASE PEX6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DPBB_PEX6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEX6_vert_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="462"/>
            <end position="597"/>
        </location>
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    <feature type="domain" description="AAA+ ATPase" evidence="21">
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            <begin position="736"/>
            <end position="874"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030576.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="980" mass="103778" checksum="0CD4E71BA0FB9149" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="370" mass="42578" checksum="B410BF86898970B5" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKAFDYVDYNKLWKILKEMGIPEHLICFLRNLYAGQEATVKTGHGQTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSILKSRDITLPTKICLVKAMVFPVVVYGCESWTVKKAEGRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISFEGMMLKLKLQYFGHLMRRVDSLEETLMLGGTGGRRRRGRQRMRWLDGITDSMDVSLSELWELVMEAWRAAIHGVAKSWTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1M8</accession>
    <name>A0A4W2E1M8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock protein beta-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HSPB3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibitor of actin polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376267.1">
        <property type="nucleotide sequence ID" value="XM_027520466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E1M8"/>
    <dbReference type="SMR" id="A0A4W2E1M8"/>
    <dbReference type="STRING" id="30522.A0A4W2E1M8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044315.1">
        <property type="protein sequence ID" value="ENSBIXP00000032756.1"/>
        <property type="gene ID" value="ENSBIXG00000015406.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005533.1">
        <property type="protein sequence ID" value="ENSBIXP00005005857.1"/>
        <property type="gene ID" value="ENSBIXG00005011429.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879177"/>
    <dbReference type="CTD" id="8988"/>
    <dbReference type="GeneTree" id="ENSGT00940000161247"/>
    <dbReference type="OMA" id="HGPRMDE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06477">
        <property type="entry name" value="ACD_HspB3_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000046">
        <property type="entry name" value="Heat shock protein beta-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033894">
        <property type="entry name" value="HSPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47097">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47097:SF1">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="10">
        <location>
            <begin position="47"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="149" mass="16722" checksum="10020106766950AC" modified="2019-09-18" version="1">MAKIILRHLIETPVRYEQEFEARGLEDCRLDHALYALPGPTTVDLGKARAAQAPPVDAAEMPPQRGESRFQVLLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGIETKDLSAILCHDGILVVEVKDSAGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1Y0</accession>
    <name>A0A4W2E1Y0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease P protein subunit p25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RPP25</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5. POP7 forms a heterodimer with RPP25 that binds to the P3 stem loop of the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E1Y0"/>
    <dbReference type="SMR" id="A0A4W2E1Y0"/>
    <dbReference type="STRING" id="30522.A0A4W2E1Y0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044640.1">
        <property type="protein sequence ID" value="ENSBIXP00000033197.1"/>
        <property type="gene ID" value="ENSBIXG00000015938.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008015.1">
        <property type="protein sequence ID" value="ENSBIXP00005030409.1"/>
        <property type="gene ID" value="ENSBIXG00005010480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002564"/>
    <dbReference type="OMA" id="WENKDPQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000006">
        <property type="entry name" value="Ribonuclease P protein subunit p25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051958">
        <property type="entry name" value="Alba-like_NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516:SF5">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="12">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="139"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="199" mass="20948" checksum="56A86A368871E780" modified="2019-09-18" version="1">MENFRKVRSEEAPVGSGDEEGGPGSGPFADLAPGAVHMRVKEGSKIRNLLAFATASMAQPATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYQSVRELWQSLPPEPTPGQKPGEPAASLSVLKNVPSLAILLSKDALDPRQPGYQPPNPHPGPSSQPTAPTSKRSLGEPAAGESSAKRSQPEPGAAEEEQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4C4</accession>
    <name>A0A4W2E4C4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4C4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000629.1">
        <property type="protein sequence ID" value="ENSBIXP00000031590.1"/>
        <property type="gene ID" value="ENSBIXG00000013870.1"/>
    </dbReference>
    <dbReference type="OMA" id="IMSWQID"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="27"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="359" mass="40585" checksum="E5D68820F4CF2500" modified="2019-09-18" version="1">MNKASGGDGIPVELFQIQDDAVKVLHSICQQIWKTQDWKRSVFTSIPKIGNAKECSNYCTIALISHASKVMLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANIRWIIKKAREFQKDIYFCFIDYAKAFDCVDHNKLWKILKEIGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEHIMRNAGLEEAQAGIKIAGRNINHLRYADDTTLMAESEEELKSLLMKVKEESEKVGVKLNIQKTKIMASGPIMSWQIDGETVETVSDFIFGGSKITADGDCSHEIKRRLLLGRKVMTDLDSIFKSRDITLPTKVCLVKAMVFPVVMYGCESWTVKKAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E587</accession>
    <name>A0A4W2E587_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904956</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E587"/>
    <dbReference type="STRING" id="30522.A0A4W2E587"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022269.1">
        <property type="protein sequence ID" value="ENSBIXP00000034480.1"/>
        <property type="gene ID" value="ENSBIXG00000017477.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005050013.1">
        <property type="protein sequence ID" value="ENSBIXP00005019866.1"/>
        <property type="gene ID" value="ENSBIXG00005023049.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286967"/>
    <dbReference type="OMA" id="VNHHSFI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF53">
        <property type="entry name" value="OLFACTORY RECEPTOR 51H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="315" mass="35488" checksum="6E44CB02D61AAA4C" modified="2019-09-18" version="1">MNTSSSHVNHHSFILTGIPGMPDKNPWMAFPLGFLYTMTLLGNGTILAIIKVDRSLHEPMYYFLSILALTDVGLSMSTLPSMLSIFWFNALEVPFDACITQMFFIHSFGEVESGVLVSMAFDRFVAIRDPLHYASILTHGIIGKIGIAVIIRAVCVVFPVPFLIKRLPFCHSNVLSHSYCLHQDAMRLACASTRVNSLYGLIVVIFTLGFDAFIILFSYVLILKTVLDIASRAERLKALNTCLSHICAVLLFYFPLIGVTMIHRFGKHLSPVVHTFMANTYLLLPPLLNPVVYSVKTKQIQRRIIHVFQRRKNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6T5</accession>
    <name>A0A4W2E6T5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Collagen alpha-1(XII) chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">COL12A1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Trimer of identical chains each containing 190 kDa of non-triple-helical sequences.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2E6T5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017650.1">
        <property type="protein sequence ID" value="ENSBIXP00000032496.1"/>
        <property type="gene ID" value="ENSBIXG00000001941.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035987">
        <property type="term" value="P:endodermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd01482">
        <property type="entry name" value="vWA_collagen_alphaI-XII-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000018">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000489">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000554">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000639">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000704">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000816">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000008">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000121">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000480">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF17">
        <property type="entry name" value="COLLAGEN ALPHA-1(XII) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021450317" description="Collagen alpha-1(XII) chain" evidence="17">
        <location>
            <begin position="24"/>
            <end position="3077"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="27"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="140"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="336"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="440"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="634"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="725"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="817"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="907"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="999"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1089"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="1199"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1387"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1477"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1568"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1658"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1757"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1852"/>
            <end position="1937"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1938"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="2029"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="2120"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="2209"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="2325"/>
            <end position="2498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1076"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2295"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2752"/>
            <end position="2898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2933"/>
            <end position="2981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1079"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2301"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2786"/>
            <end position="2796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2819"/>
            <end position="2828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2830"/>
            <end position="2839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2855"/>
            <end position="2864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2866"/>
            <end position="2876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2943"/>
            <end position="2952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="3077" mass="335470" checksum="4C1FB0DEAC710D29" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8L4</accession>
    <name>A0A4W2E8L4_BOBOX</name>
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        <submittedName>
            <fullName evidence="10">Solute carrier family 22 member 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC22A8</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.</text>
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    <dbReference type="STRING" id="30522.A0A4W2E8L4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000028046.1"/>
        <property type="gene ID" value="ENSBIXG00000009114.1"/>
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        <property type="component" value="Chromosome 29"/>
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        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 22 member 6"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
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    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
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    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004749">
        <property type="entry name" value="Orgcat_transp/SVOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
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        <property type="entry name" value="2A0119"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="144"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="238"/>
            <end position="257"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
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    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="83"/>
            <end position="498"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="515"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="518"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="543" mass="59523" checksum="3D39E33DD5A1BB1C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB15</accession>
    <name>A0A4W2EB15_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM187 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EB15"/>
    <dbReference type="STRING" id="30522.A0A4W2EB15"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019195.1">
        <property type="protein sequence ID" value="ENSBIXP00000033153.1"/>
        <property type="gene ID" value="ENSBIXG00000015881.1"/>
    </dbReference>
    <dbReference type="OMA" id="AWDKDST"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039311">
        <property type="entry name" value="FAM187A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32178">
        <property type="entry name" value="FAM187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32178:SF7">
        <property type="entry name" value="IG-LIKE V-TYPE DOMAIN-CONTAINING PROTEIN FAM187A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021254226" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="268"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="421" mass="48434" checksum="C30D00AF71FB2AA7" modified="2019-09-18" version="1">MNLAHTTVLLWAWGSLQAFEIVEKENIFQRTPCPAFLMFDNAAYLTDMSFELPCPCKPEEVSAVVWYYQKHLGSSHTKVLTDFDGRVLTEAAQVRVGSDMLVRFSIRMFSLLVFRAQPEDSGLYFCGTRKGDYFYAYDVDIQSSEGMVATFKDQGQEPLEDEYHGSLRVFTTFWEWTPCDRCGVRGEQWRIGLCYLQSPDLSPRYRKILPNVVSCGSRAVPRQLRAKASDHNPELLVRSCLMPCEKKKKVQEGVMAIFNYVSKVGSRPWLPQVPIQFHQQRLGHGLIISCPGARPEHAVAWDKDHQYLYRTQYLKGVNGSMRVFIDHGNHLHIRFTQLEDRGIYYCWRQGERIAGFRLGVTSPGRYPVSFSDPETRAALGLILIGYMLITVIFISIHLCRCCCYLFRFCPNFSPRLSRPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBV9</accession>
    <name>A0A4W2EBV9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Complement C1q tumor necrosis factor-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Collagenous repeat-containing sequence 26 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Secretory protein CORS26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">C1QTNF3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027376114.1">
        <property type="nucleotide sequence ID" value="XM_027520313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBV9"/>
    <dbReference type="STRING" id="30522.A0A4W2EBV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044792.1">
        <property type="protein sequence ID" value="ENSBIXP00000033416.1"/>
        <property type="gene ID" value="ENSBIXG00000016188.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014579.1">
        <property type="protein sequence ID" value="ENSBIXP00005001258.1"/>
        <property type="gene ID" value="ENSBIXG00005029341.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879067"/>
    <dbReference type="CTD" id="114899"/>
    <dbReference type="GeneTree" id="ENSGT00940000161378"/>
    <dbReference type="OMA" id="HMLALFF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035356">
        <property type="term" value="P:intracellular triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032715">
        <property type="term" value="P:negative regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071638">
        <property type="term" value="P:negative regulation of monocyte chemotactic protein-1 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901223">
        <property type="term" value="P:negative regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070165">
        <property type="term" value="P:positive regulation of adiponectin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000006">
        <property type="entry name" value="complement C1q tumor necrosis factor-related protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF52">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611066" description="Complement C1q tumor necrosis factor-related protein 3" evidence="9">
        <location>
            <begin position="23"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="10">
        <location>
            <begin position="186"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="319" mass="34953" checksum="FF1C05E0C4729D15" modified="2019-09-18" version="1">MLGRQLVYWHLLALLFLPFCLCQDEYMEVSGRANKVVARIVQSHQQTGRSGSRREKVRERSHPRTGTVDNNTSTDLKSLRPDELPPPEVDDLAQITPFWGQSPQTGGLPPDCSKCCHGDYSFRGYQGPPGPPGPPGIPGNHGNNGNNGATGHEGAKGEKGDKGDLGPRGERGQHGPKGEKGYPGIPPELQIAFMASLATHFTNQNSGIIFSSVETNIGNFFDVMTGRFGAPVSGVYFFTFSMMKHEDVEEVYVYLMHNGNTVFSMYSYETKGKSDTSSNHAVLKLAKGDEVWLRMGNGALHGDHQRFSTFAGFLLFETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBZ6</accession>
    <name>A0A4W2EBZ6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBZ6"/>
    <dbReference type="STRING" id="30522.A0A4W2EBZ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007494.1">
        <property type="protein sequence ID" value="ENSBIXP00000029335.1"/>
        <property type="gene ID" value="ENSBIXG00000010920.1"/>
    </dbReference>
    <dbReference type="OMA" id="MDWLQLF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016412">
        <property type="term" value="F:serine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF3">
        <property type="entry name" value="GHRELIN O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="436" mass="49309" checksum="96FC4FEA29F591AA" modified="2019-09-18" version="1">MMDWLQLFFLDPVSLYQGAAFPFALLFNHLCVMDSFSTQARYLFLLAGGSALAVAAMGAFAVLVFIPALCTVVLIHSLGPQDVHRPTFLFQMTWQTLCHLGLHYTEYYLQEAPSTRFCITLSSLMLLTQKITSLSLDIREGKVVAPSGRIPNKNSLSEHLHAALPYLSYLLFFPALLGGPLCSFQRFQARVEGSSSLWSRHSFWALTWRALQILGLESLKVIVSGVVGVGAGLGGCRQLQCVFVLWSTAGLFKLTYYSHWLLDDALLRAAGFGSELGRSPGEEGLLPDADIWTLETTHRIALFARKWNQSTARWLRRLVFQQRRTWPLLQTFLFSAWWHGLHPGQVFGFLCWAVMVEADYLIHAFASVFISSWPMRLLYRALAWAHTQLIIAYIMLAVEARSLSSLWLLWNSYSSVFPTVYCILLLLLAKRKHKCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EC97</accession>
    <name>A0A4W2EC97_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serpin family B member 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SERPINB13</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serpin family. Ov-serpin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381954.1">
        <property type="nucleotide sequence ID" value="XM_027526153.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027381955.1">
        <property type="nucleotide sequence ID" value="XM_027526154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EC97"/>
    <dbReference type="SMR" id="A0A4W2EC97"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007159.1">
        <property type="protein sequence ID" value="ENSBIXP00000029434.1"/>
        <property type="gene ID" value="ENSBIXG00000011064.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028019.1">
        <property type="protein sequence ID" value="ENSBIXP00005016593.1"/>
        <property type="gene ID" value="ENSBIXG00005003355.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882936"/>
    <dbReference type="CTD" id="5275"/>
    <dbReference type="GeneTree" id="ENSGT00940000162214"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000071">
        <property type="entry name" value="Serpin B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF161">
        <property type="entry name" value="SERPIN B13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="13"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="363" mass="40750" checksum="E565060F8C858757" modified="2019-09-18" version="1">MDSLVPASTSFGLDLFKDLSKTDEGNVLFSPAGISTTIGMLPPMIRGATATQEQELEKTEEIHHQLQRVLSEISKPNDDYELKIANRLFGEKTYLFLQKYLDYVEKHYHASLEPVDFVNAADESRKKINSWVESQTNEKIKDLLPDGSLSSSIKLVVVNVIYFKGQWDREFKKENTKEEEFWLNKSTSKSVPMMTQRQSFSFKTLEDVPAKILGLPYRSHDLSMFVLLPNDIDGLEKIIDKITPEKLIEWTSAGRMEERAVDLHLPRFRAAGSYDLEAASAGLCAAGLAGSPVGAGLRAQRLLHRSVLEVTEAGTEAAAATAVGFAVTPAQDWERFHCNHPFLFFIRHNESDSVLFFGRFSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EE03</accession>
    <name>A0A4W2EE03_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EE03"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021617.1">
        <property type="protein sequence ID" value="ENSBIXP00000034190.1"/>
        <property type="gene ID" value="ENSBIXG00000017120.1"/>
    </dbReference>
    <dbReference type="OMA" id="HKCHICC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004137">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002239">
        <property type="entry name" value="Zinc finger protein 226"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000149">
        <property type="entry name" value="Zinc finger protein 569"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001509">
        <property type="entry name" value="Zinc finger protein 616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001442">
        <property type="entry name" value="zinc finger protein 696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF246">
        <property type="entry name" value="GENE, 19965-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
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    <sequence length="569" mass="65820" checksum="F15D0425427234A0" modified="2019-09-18" version="1"> </sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EII1"/>
    <dbReference type="SMR" id="A0A4W2EII1"/>
    <dbReference type="STRING" id="30522.A0A4W2EII1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029993.1">
        <property type="protein sequence ID" value="ENSBIXP00000037800.1"/>
        <property type="gene ID" value="ENSBIXG00000021466.1"/>
    </dbReference>
    <dbReference type="OMA" id="HAEQQDK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028192">
        <property type="entry name" value="BMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32014">
        <property type="entry name" value="BCL-2-MODIFYING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32014:SF2">
        <property type="entry name" value="BCL-2-MODIFYING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15185">
        <property type="entry name" value="BMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="184" mass="20703" checksum="51AFAC06486FE852" modified="2019-09-18" version="1">MEPPQCVEELEDDVFQPEDGEPGTQPRSLLSADLFAQSQLDCPLSRLQLFPLTHCCGPGLRPTSQEDKATQTLSPASPSQGVMLPCGVTEEPQRLFYGHAGYRLPLPASFPAGLPLGEQPPEGQWQHRAEIQIARKLQCIADQFHRLHMQQYQQNRNRMWWQILLFLHNVALNGDENRNGAGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJ93</accession>
    <name>A0A4W2EJ93_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJ93"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028899.1">
        <property type="protein sequence ID" value="ENSBIXP00000037342.1"/>
        <property type="gene ID" value="ENSBIXG00000007397.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="346" mass="39834" checksum="588FFD5FCB1982E4" modified="2019-09-18" version="1">MLKILQARLQQYMNCELPDVQAGYRKGRGTRDQIANICWIIEKAREFQKNIYFCFTDYAKAFDCVDHNKLWKILKEMGIPDHLTCFLRNLYAGQEATVRTGHGTTDWFQIGKGVYQGCIFSPCLFNLYAEYIMRNAGLEEAQAGIEIAGRNINNLRYADDTTLLAESEEELKSLLMKVKEETEKVGLKLNIQKTKIMASGPITSWEIDGETVETVETVSDFIFWSSKITADGDCSHEIKRCLLLGRKIMTNLDSILKSRDITLPTKVRLVKAMVFPVVLYGCESWTVKQTEHQRIDAFELWCWRRLLRVPWTARRSNPSTLKEICPGCSLEGLMLRLKLQYFGHVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EL60</accession>
    <name>A0A4W2EL60_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EL60"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046704.1">
        <property type="protein sequence ID" value="ENSBIXP00000036069.1"/>
        <property type="gene ID" value="ENSBIXG00000019428.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="377" mass="43686" checksum="789905E6363E1E41" modified="2019-09-18" version="1">MLHSICQQIWKTQQWPLDWKRSVFIPIPKKGNAKECSNYRTIALISHTSKVMLKILQARLQQYVNPELPDVQAGFRKGKGTRDQIANIRWIMEKAREFQKNIYFCFIDYAQVFDCVDHNKLWKILKEMGIPDHLICLLKNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMPENEEELKSLLMKVKVESEKVGLKLNIHKMKIMAPGPITSWEIDGETVETVSDFIFWGSQITADGDCSQEIKRRLLLGRKVMTNLDSIFKIRDVILPTKVCLVKAMVFPVVMYGYESWTVKKAECRRIDAFELWCWRRLLRVPWTTRRSNQSILKEISPGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPG6</accession>
    <name>A0A4W2EPG6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CNN3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027392249.1">
        <property type="nucleotide sequence ID" value="XM_027536448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPG6"/>
    <dbReference type="SMR" id="A0A4W2EPG6"/>
    <dbReference type="STRING" id="30522.A0A4W2EPG6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032229.1">
        <property type="protein sequence ID" value="ENSBIXP00000038763.1"/>
        <property type="gene ID" value="ENSBIXG00000002782.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011920.1">
        <property type="protein sequence ID" value="ENSBIXP00005002594.1"/>
        <property type="gene ID" value="ENSBIXG00005005360.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889590"/>
    <dbReference type="CTD" id="1266"/>
    <dbReference type="GeneTree" id="ENSGT00940000154539"/>
    <dbReference type="OMA" id="YHSEYQD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21284">
        <property type="entry name" value="CH_CNN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000040">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF17">
        <property type="entry name" value="CALPONIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
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</entry>
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</entry>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="127" mass="14084" checksum="FCC09789B18317CF" modified="2019-09-18" version="1">MGNQVICCVALCLFGAGTVAGGVTQTPKYLLKKEGGALTLECEQDFDYDSMYWYRQDPGLGLRRIFFSQFVNTVQKEDIAKGYSASREKKPFFPLTVTSAQKNQTALYLCAASRDTVTQSHLLSAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ES75</accession>
    <name>A0A4W2ES75_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ephrin type-B receptor 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Tyrosine-protein kinase-defective receptor EPH-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">EPHB6</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027394594.1">
        <property type="nucleotide sequence ID" value="XM_027538793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ES75"/>
    <dbReference type="SMR" id="A0A4W2ES75"/>
    <dbReference type="STRING" id="30522.A0A4W2ES75"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049296.1">
        <property type="protein sequence ID" value="ENSBIXP00000039629.1"/>
        <property type="gene ID" value="ENSBIXG00000023545.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009121.1">
        <property type="protein sequence ID" value="ENSBIXP00005004043.1"/>
        <property type="gene ID" value="ENSBIXG00005010085.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891081"/>
    <dbReference type="CTD" id="2051"/>
    <dbReference type="GeneTree" id="ENSGT00940000160399"/>
    <dbReference type="OMA" id="PQGPSCM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050798">
        <property type="term" value="P:activated T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000525">
        <property type="term" value="P:positive regulation of T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001806">
        <property type="term" value="P:type IV hypersensitivity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10475">
        <property type="entry name" value="EphR_LBD_B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ephrin type-A receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ephrin type-B receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ephrin type-B receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ephrin type-B receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000004">
        <property type="entry name" value="ephrin type-B receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000143">
        <property type="entry name" value="Ephrin type-B receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF15">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
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    </dbReference>
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        <property type="entry name" value="SAM_DOMAIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 18" id="KW-0732">Signal</keyword>
    <keyword evidence="2 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611171" description="Ephrin type-B receptor 6" evidence="18">
        <location>
            <begin position="32"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="581"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="22">
        <location>
            <begin position="33"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="360"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="473"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="656"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="20">
        <location>
            <begin position="934"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="662"/>
            <end position="670"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="75"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059300"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062552"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1007" mass="108802" checksum="646602AFFFEBD484" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUY7</accession>
    <name>A0A4W2EUY7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL1m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EUY7"/>
    <dbReference type="STRING" id="30522.A0A4W2EUY7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036808.1">
        <property type="protein sequence ID" value="ENSBIXP00000040768.1"/>
        <property type="gene ID" value="ENSBIXG00000024907.1"/>
    </dbReference>
    <dbReference type="OMA" id="MWDKSSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000003">
        <property type="entry name" value="39S ribosomal protein L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005879">
        <property type="entry name" value="Ribosomal_uL1_mit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01170">
        <property type="entry name" value="rplA_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="325" mass="36630" checksum="2EA84C95F6DD1365" modified="2019-09-18" version="1">MAATVRCFGRVLIHHQRCSLATVTSQTSLYPCCIYVPVPNRHFAAAAKPATKTKKGTKEKASNEKKDDIEKIKSYPFMEGEPEDDVYLKRLYPRQIYEVEKAVNLLKKFQVLDFTNPKQGVYLDLTLDMTLGKKKKVEPFASVLSLPYPFISEMSKVAVFTGNASEIKIAEENGAAFAGGTNLIQKILDDEIQADFYIAVPEIMPELNPLRKKLKTRFPKFNRNSVGRDIPKMLELFKTGLEIKVDEERENFLETKIATLDMPSDQIAANLQAVINEVCRQRPLNLGPFVVRAFLRSSTSEGLLLKIEPLLPKEGETKESDKKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EW05</accession>
    <name>A0A4W2EW05_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EW05"/>
    <dbReference type="STRING" id="30522.A0A4W2EW05"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037786.1">
        <property type="protein sequence ID" value="ENSBIXP00000041215.1"/>
        <property type="gene ID" value="ENSBIXG00000025389.1"/>
    </dbReference>
    <dbReference type="OMA" id="WMLDELT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000051">
        <property type="entry name" value="40S ribosomal protein S4, X isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS4 N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 central region" evidence="8">
        <location>
            <begin position="95"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 C-terminal" evidence="10">
        <location>
            <begin position="212"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="263" mass="29398" checksum="5FD8B87BDB0D7083" modified="2019-09-18" version="1">MARGPKKHLKYVAAPKHWMLDELTGVFAPRPSTGPHKLRECLPLFIFLRNRLKYALTGDEVKKICMQRFTKIDGKVCTDITYPAGFMDVISIDKTGENFHLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNGTIQIDLETGKITDFIKFDTGNLCMVTGGASLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVFGKGNKPWISLPCGKGICLTIAEERDKRLAAKQSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZA9</accession>
    <name>A0A4W2EZA9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Family with sequence similarity 13 member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FAM13A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZA9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040349.1">
        <property type="protein sequence ID" value="ENSBIXP00000042474.1"/>
        <property type="gene ID" value="ENSBIXG00000026541.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="entry name" value="FAM13"/>
    </dbReference>
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        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
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        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF18">
        <property type="entry name" value="PROTEIN FAM13A"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM13A-like" evidence="4">
        <location>
            <begin position="621"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="622"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="103"/>
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    </feature>
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        <location>
            <begin position="146"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="197"/>
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            <begin position="398"/>
            <end position="407"/>
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            <begin position="412"/>
            <end position="422"/>
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        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF26116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="697" mass="80067" checksum="D9712209BE79F878" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2 6">Belongs to the CREG family.</text>
    </comment>
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    </dbReference>
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        <property type="entry name" value="Split_barrel_FMN-bd"/>
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        <property type="entry name" value="CREG1 PROTEIN"/>
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    </dbReference>
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        <location>
            <begin position="32"/>
            <end position="221"/>
        </location>
    </feature>
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        <location>
            <begin position="51"/>
            <end position="217"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="221" mass="23973" checksum="D3B39D2183B8834A" modified="2019-09-18" version="1">MAGCACGFVRAVLAFLLAPALVTLLVAPARGRGGRGHGDWDAAELLLPPLPPRENAALVARFVTNLCDWGALATISTQEGVRGRPFADVLSLSDGPPGKGSGVPYFYLSPLQQSVGNLQENPYATLTMSLAETNFCRKYGFDPQSPLCAHIILSGTVIKVSETEMDVAKNSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPAEYYNATSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F532</accession>
    <name>A0A4W2F532_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F532"/>
    <dbReference type="STRING" id="30522.A0A4W2F532"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052819.1">
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        <property type="gene ID" value="ENSBIXG00000005932.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Putative e3 ubiquitin-protein ligase march8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45981">
        <property type="entry name" value="LD02310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="23">
        <location>
            <begin position="320"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="328"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="88"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="537" mass="60138" checksum="0E68FFE3F94E5843" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FIH1</accession>
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        <recommendedName>
            <fullName evidence="12">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SNX33</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376592.1">
        <property type="nucleotide sequence ID" value="XM_027520791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FIH1"/>
    <dbReference type="SMR" id="A0A4W2FIH1"/>
    <dbReference type="STRING" id="30522.A0A4W2FIH1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002866.1">
        <property type="protein sequence ID" value="ENSBIXP00000006909.1"/>
        <property type="gene ID" value="ENSBIXG00000012964.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006500.1">
        <property type="protein sequence ID" value="ENSBIXP00005005365.1"/>
        <property type="gene ID" value="ENSBIXG00005011075.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879425"/>
    <dbReference type="CTD" id="257364"/>
    <dbReference type="GeneTree" id="ENSGT00940000160162"/>
    <dbReference type="OMA" id="QAFQMDP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:endosome organization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0044351">
        <property type="term" value="P:macropinocytosis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:mitotic cytokinesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR037427">
        <property type="entry name" value="SNX33_BAR"/>
    </dbReference>
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        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019497">
        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
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        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF3">
        <property type="entry name" value="SORTING NEXIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10456">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027744">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF64268">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
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    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="227"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="67"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="571" mass="65224" checksum="AA998BB0753C118C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FPM6</accession>
    <name>A0A4W2FPM6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="20">Ubiquitin carboxyl-terminal hydrolase 39</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="21">U4/U6.U5 tri-snRNP-associated 65 kDa protein</fullName>
        </alternativeName>
    </protein>
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        <name evidence="26" type="primary">USP39</name>
    </gene>
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        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 29">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">The U4/U6-U5 tri-snRNP complex is a building block of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027411338.1">
        <property type="nucleotide sequence ID" value="XM_027555537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FPM6"/>
    <dbReference type="SMR" id="A0A4W2FPM6"/>
    <dbReference type="STRING" id="30522.A0A4W2FPM6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019383.1">
        <property type="protein sequence ID" value="ENSBIXP00000033241.1"/>
        <property type="gene ID" value="ENSBIXG00000015989.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004126.1">
        <property type="protein sequence ID" value="ENSBIXP00005006574.1"/>
        <property type="gene ID" value="ENSBIXG00005012067.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901310"/>
    <dbReference type="CTD" id="10713"/>
    <dbReference type="GeneTree" id="ENSGT00390000007992"/>
    <dbReference type="OMA" id="QLRRFKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000068">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000030">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="25">
        <location>
            <begin position="103"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="225"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="565" mass="65337" checksum="16C4D75746EC311E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FT69</accession>
    <name>A0A4W2FT69_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox C8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HOXC8</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398877.1">
        <property type="nucleotide sequence ID" value="XM_027543076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FT69"/>
    <dbReference type="SMR" id="A0A4W2FT69"/>
    <dbReference type="STRING" id="30522.A0A4W2FT69"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032726.1">
        <property type="protein sequence ID" value="ENSBIXP00000019217.1"/>
        <property type="gene ID" value="ENSBIXG00000004077.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016120.1">
        <property type="protein sequence ID" value="ENSBIXP00005008595.1"/>
        <property type="gene ID" value="ENSBIXG00005013635.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893232"/>
    <dbReference type="CTD" id="3224"/>
    <dbReference type="GeneTree" id="ENSGT00940000161194"/>
    <dbReference type="OMA" id="PSIMFPW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048705">
        <property type="term" value="P:skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000072">
        <property type="entry name" value="Homeobox protein Hox-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050948">
        <property type="entry name" value="Antp_homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46166">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46166:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="206"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="242" mass="27740" checksum="4A2A53BDA4CFA878" modified="2019-09-18" version="1">MSSYFVNPLFSKYKGGESLEPAYYDCRFPQSVGRSHALVYGPGGSAPGFQHASHHVQDFFHHGTSGISNSGYQQNPCSLSCHGDASKFYGYEALPRQSLYGAQQEASVVQYPDCKSSANTNSSEGQGHLNQNSSPSLMFPWMRPHAPGRRSGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKLPGARDEEKVEEEGNEEEEKEEEEKEENKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FW90</accession>
    <name>A0A4W2FW90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase</fullName>
            <ecNumber evidence="5">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">4'-phosphopantetheinyl transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">AASDHPPT</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A, regardless of whether the CoA is presented in the free thiol form or as an acetyl thioester, to a serine residue of a broad range of acceptors including the acyl carrier domain of FASN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>apo-[ACP] + CoA = holo-[ACP] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>apo-[ACP] + acetyl-CoA = acetyl-[ACP] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:46565"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027419790.1">
        <property type="nucleotide sequence ID" value="XM_027563989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FW90"/>
    <dbReference type="SMR" id="A0A4W2FW90"/>
    <dbReference type="STRING" id="30522.A0A4W2FW90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004861.1">
        <property type="protein sequence ID" value="ENSBIXP00000005883.1"/>
        <property type="gene ID" value="ENSBIXG00000012022.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018440.1">
        <property type="protein sequence ID" value="ENSBIXP00005010177.1"/>
        <property type="gene ID" value="ENSBIXG00005014858.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905956"/>
    <dbReference type="CTD" id="60496"/>
    <dbReference type="GeneTree" id="ENSGT00390000004663"/>
    <dbReference type="OMA" id="WVFEESL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000006">
        <property type="entry name" value="L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055066">
        <property type="entry name" value="AASDHPPT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF10">
        <property type="entry name" value="L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22624">
        <property type="entry name" value="AASDHPPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611472" description="L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase" evidence="16">
        <location>
            <begin position="21"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="4'-phosphopantetheinyl transferase N-terminal" evidence="18">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="17">
        <location>
            <begin position="125"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="309" mass="35687" checksum="F2671D6AF09DE0FA" modified="2019-09-18" version="1">MVLLAWRLCVLPAMEGVRWAFPCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAALAGRLMIRKLVAEKLNIPWNNIRLQRTAKGKPVLAKDSLNPYPNFNFNISHQGDYAVLAAESELQVGIDIMKTSFPGRGSIPEFFHIMKRKFTDKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDISPLNLDIGQVYKETRLFLDGEEEKEWAFEESKIDEHHFVAVALRKPNGSRHQNVTSQDDSKATQRQFTILTFNDLISSAVPMTPEDPSFWDCFCFTEEISIRNGTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G1C8</accession>
    <name>A0A4W2G1C8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mex-3 RNA binding family member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MEX3B</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027377301.1">
        <property type="nucleotide sequence ID" value="XM_027521500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G1C8"/>
    <dbReference type="STRING" id="30522.A0A4W2G1C8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000696.1">
        <property type="protein sequence ID" value="ENSBIXP00000007983.1"/>
        <property type="gene ID" value="ENSBIXG00000001522.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046037.1">
        <property type="protein sequence ID" value="ENSBIXP00005012165.1"/>
        <property type="gene ID" value="ENSBIXG00005002623.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879746"/>
    <dbReference type="CTD" id="84206"/>
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        <property type="component" value="Chromosome 21"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:P-body"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="KH-I_MEX3_rpt1"/>
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        <property type="entry name" value="RING-HC_MEX3B"/>
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        <property type="entry name" value="Mex-3 RNA-binding family member B"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Mex-3 RNA-binding family member D"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="K Homology domain, type 1"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="RING FINGER AND KH DOMAIN CONTAINING PROTEIN 1"/>
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        <property type="entry name" value="RNA-BINDING PROTEIN MEX3B"/>
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        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="536"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="259"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="359"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="497"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="501"/>
            <end position="526"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="587" mass="59778" checksum="66C1FA261C458C26" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GE66</accession>
    <name>A0A4W2GE66_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor SPF27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BCAS2</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPF27 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027536000.1"/>
    </dbReference>
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        <property type="gene ID" value="ENSBIXG00000023982.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005015185.1"/>
        <property type="gene ID" value="ENSBIXG00005002655.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:precatalytic spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:Prp19 complex"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:oocyte development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:ovarian follicle development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296">
        <property type="entry name" value="BCAS2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SPF27"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="139"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005015185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="225" mass="26120" checksum="96629B175B696890" modified="2019-09-18" version="1">MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESTWVSLVSKNYEIERTIVQLENELFQMKQQHGEANKENIRQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GE83</accession>
    <name>A0A4W2GE83_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">NEDD8-activating enzyme E1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NAE1</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372164.1">
        <property type="nucleotide sequence ID" value="XM_027516363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GE83"/>
    <dbReference type="STRING" id="30522.A0A4W2GE83"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018307.1">
        <property type="protein sequence ID" value="ENSBIXP00000009810.1"/>
        <property type="gene ID" value="ENSBIXG00000015422.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028065.1">
        <property type="protein sequence ID" value="ENSBIXP00005016633.1"/>
        <property type="gene ID" value="ENSBIXG00005020228.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876767"/>
    <dbReference type="CTD" id="8883"/>
    <dbReference type="GeneTree" id="ENSGT00550000074901"/>
    <dbReference type="OMA" id="KLITHQY"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043523">
        <property type="term" value="P:regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150052">
        <property type="term" value="P:regulation of postsynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01493">
        <property type="entry name" value="APPBP1_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000174">
        <property type="entry name" value="NEDD8-activating enzyme E1 regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000187">
        <property type="entry name" value="NEDD8-activating enzyme E1 regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030667">
        <property type="entry name" value="APP-BP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF29">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039099">
        <property type="entry name" value="APP-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="8">
        <location>
            <begin position="13"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="541" mass="61228" checksum="7F0D36A835471B38" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GHA1</accession>
    <name>A0A4W2GHA1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phagophore assembly factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PHAF1</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GHA1"/>
    <dbReference type="STRING" id="30522.A0A4W2GHA1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018405.1">
        <property type="protein sequence ID" value="ENSBIXP00000032812.1"/>
        <property type="gene ID" value="ENSBIXG00000015499.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029269.1">
        <property type="protein sequence ID" value="ENSBIXP00005017408.1"/>
        <property type="gene ID" value="ENSBIXG00005020907.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153528"/>
    <dbReference type="OMA" id="YRKNDQK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="422" mass="47592" checksum="997079E24CD8ECF1" modified="2019-09-18" version="1">MLDLEVVPERSLGNEQWEFALGMPLAQAVAILQKHCRIIKNVQVLYSEQSPLSHDLILNLTQDGIKLLFDAFNQRLKVIEVYDLTKVKLKYCGVHFNSQAIAPTIEQIDQSFGATHPGVYNSAEQLFHLNFRGLSFSFQLDSWTEAPKYEPNFAHGLASLQIPHGATVKRMYIYSGNSLQDTKAPVMPLSCFLGNVYAESVDVLRDGTGPSGLRLRLLAAGCGPGLLADAKMRVFERSVYFGDSCQDVLSMLGSPHKVFYKSEDKMKIHSPSPHKQVPSKCNDYFFNYFTLGVDILFDANTHKVKKFVLHTNYPGHYNFNIYHRCEFKIPLTVKRENTEGQTETCTTYSKWDNIQELLGHPVEKPVVLHRSSSPNNTNPFGSTFCFGLQRMIFEVMQNNHIASVTLYGPPRPGAHLRTAELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HEF8</accession>
    <name>A0A4W2HEF8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit NDUFA4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the complex IV NDUFA4 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HEF8"/>
    <dbReference type="SMR" id="A0A4W2HEF8"/>
    <dbReference type="STRING" id="30522.A0A4W2HEF8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015038.1">
        <property type="protein sequence ID" value="ENSBIXP00000000983.1"/>
        <property type="gene ID" value="ENSBIXG00000029133.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011065.1">
        <property type="protein sequence ID" value="ENSBIXP00005029345.1"/>
        <property type="gene ID" value="ENSBIXG00005009270.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154268"/>
    <dbReference type="OMA" id="VSWGRKN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256:SF4">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT NDUFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="88" mass="9983" checksum="A7AA4AF099160C22" modified="2019-09-18" version="1">MICRTVSQAKKHPSLIPFFLFIGAVGTGAALYVLHLALFNPLLNNNDSWNKLGPNGQYKFYSVNVDYSKLKKEGPDSYMKRFTIKLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HIV2</accession>
    <name>A0A4W2HIV2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TGFB induced factor homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TGIF2</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TALE/TGIF homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414419.1">
        <property type="nucleotide sequence ID" value="XM_027558618.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414420.1">
        <property type="nucleotide sequence ID" value="XM_027558619.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414421.1">
        <property type="nucleotide sequence ID" value="XM_027558620.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414422.1">
        <property type="nucleotide sequence ID" value="XM_027558621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HIV2"/>
    <dbReference type="SMR" id="A0A4W2HIV2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030441.1">
        <property type="protein sequence ID" value="ENSBIXP00000017772.1"/>
        <property type="gene ID" value="ENSBIXG00000021714.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006209.1">
        <property type="protein sequence ID" value="ENSBIXP00005030967.1"/>
        <property type="gene ID" value="ENSBIXG00005011217.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903064"/>
    <dbReference type="CTD" id="60436"/>
    <dbReference type="GeneTree" id="ENSGT00940000159849"/>
    <dbReference type="OMA" id="ICHSTSP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000059">
        <property type="entry name" value="TGFB-induced factor homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="237" mass="25768" checksum="FB61840D89659ED4" modified="2019-09-18" version="1">MSDSDLGEEEGLLSLAGKRKRRGNLPKESVKILRDWLYLHRYNAYPSEQEKLSLSGQTNLSVLQICNWFINARRRLLPDMLRKDGKDPNQYTISRRGGKASDVALPRGGSPSVLAVSVPAPTNVLSLSMCSMPLHSGQGDKPGAAFPQGELEPPKPLVTPGSTLTLLTRAEAGSPTGGLFNTPPPTPPEQDKEDFSGFQLLVEVALQRAAEMELQKQQNPSPALLRTPIALVPENPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HS09</accession>
    <name>A0A4W2HS09_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZNF3</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383521.1">
        <property type="nucleotide sequence ID" value="XM_027527720.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383522.1">
        <property type="nucleotide sequence ID" value="XM_027527721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HS09"/>
    <dbReference type="STRING" id="30522.A0A4W2HS09"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053276.1">
        <property type="protein sequence ID" value="ENSBIXP00000026509.1"/>
        <property type="gene ID" value="ENSBIXG00000029904.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000438.1">
        <property type="protein sequence ID" value="ENSBIXP00005032866.1"/>
        <property type="gene ID" value="ENSBIXG00005013463.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883752"/>
    <dbReference type="CTD" id="7551"/>
    <dbReference type="GeneTree" id="ENSGT00940000153104"/>
    <dbReference type="OMA" id="MGDRPHK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004137">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000824">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000944">
        <property type="entry name" value="zinc finger protein 232 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF366">
        <property type="entry name" value="ZINC FINGER PROTEIN ZFP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="12">
        <location>
            <begin position="52"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="447" mass="50444" checksum="272C74A9F9F52D0E" modified="2019-09-18" version="1">METQADRVSQEPQALLESALPSSKGPTFSDKGGLGDEMLAAALLKAKAQELVTFEDVAVYFIRKEWKRLEPAQRDLYRDVMLENYGNVFSLDGETRTEPDSEISEDRGSHGVLLGRFQKDISQGLKFEEAYEQEVSLKRQLGSSSGERLNRKMQEFGQVAAEEQRAPTGGRSEKCGDLRDSFTVSANLISHQRLPVGDRPHKCDECSKSFNRTSDLIQHQRIHTGEKPYECSECGKAFSQSSHLIQHQRIHTGEKPYECNDCGKTFSCSSALILHRRIHTGEKPYECNECGKTFSWSSTLTHHQRIHTGEKPYACNECGKAFSRSSTLIHHQRIHTGEKPYECNECGKAFSQSSHLYQHQRIHTGEKPYECMECGGKFTYSSGLIQHQRIHTGENPYECSECGKAFRYSSALVRHQRIHTGEKPLNVMGVSKSSLQVTAEVNIREST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HV14</accession>
    <name>A0A4W2HV14_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Syntaxin-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein unc-18 homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein unc-18 homolog A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">STXBP1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027412136.1">
        <property type="nucleotide sequence ID" value="XM_027556335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HV14"/>
    <dbReference type="SMR" id="A0A4W2HV14"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027620.1">
        <property type="protein sequence ID" value="ENSBIXP00000036779.1"/>
        <property type="gene ID" value="ENSBIXG00000020273.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021058.1">
        <property type="protein sequence ID" value="ENSBIXP00005035165.1"/>
        <property type="gene ID" value="ENSBIXG00005016295.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901667"/>
    <dbReference type="CTD" id="6812"/>
    <dbReference type="GeneTree" id="ENSGT00940000155127"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903305">
        <property type="term" value="P:regulation of regulated secretory pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="594" mass="67569" checksum="2DD0715F875CE0F3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I0M5</accession>
    <name>A0A4W2I0M5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CCL17</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes. Acts by binding to CCR4 at T-cell surface. Mediates GM-CSF/CSF2-driven pain and inflammation. In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation. Plays a role in wound healing, mainly by inducing fibroblast migration into the wound.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027370326.1">
        <property type="nucleotide sequence ID" value="XM_027514525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I0M5"/>
    <dbReference type="SMR" id="A0A4W2I0M5"/>
    <dbReference type="STRING" id="30522.A0A4W2I0M5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017570.1">
        <property type="protein sequence ID" value="ENSBIXP00000009345.1"/>
        <property type="gene ID" value="ENSBIXG00000001918.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022294.1">
        <property type="protein sequence ID" value="ENSBIXP00005035719.1"/>
        <property type="gene ID" value="ENSBIXG00005016945.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875867"/>
    <dbReference type="CTD" id="6361"/>
    <dbReference type="GeneTree" id="ENSGT01100000263482"/>
    <dbReference type="OMA" id="CCLDYFK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000012">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF111">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4 10" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516566" description="C-C motif chemokine" evidence="10">
        <location>
            <begin position="24"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="11">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="102" mass="11102" checksum="731E84BE8D9BB565" modified="2019-09-18" version="1">MAPLKTVLLVAVLLGALLQDTHAARAGNVGRECCLQFYKGSIPQKVLVGWYQTSDDCPNKAIVLVTRSGRTICANPKDKTVKKAMKYLQKQKKSPASALQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I6Q0</accession>
    <name>A0A4W2I6Q0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CYB5A</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I6Q0"/>
    <dbReference type="STRING" id="30522.A0A4W2I6Q0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013992.1">
        <property type="protein sequence ID" value="ENSBIXP00000001454.1"/>
        <property type="gene ID" value="ENSBIXG00000028540.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031140.1">
        <property type="protein sequence ID" value="ENSBIXP00005039586.1"/>
        <property type="gene ID" value="ENSBIXG00005022055.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156770"/>
    <dbReference type="OMA" id="AFDDFGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF154">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="11">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="98" mass="11182" checksum="469592F435F2C9FE" modified="2019-09-18" version="1">MAEESSKAVKYYTLEEIQKHNNSKSTWLILHYKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDARELSKTFIIGELHPDDRSKITKPSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IBF3</accession>
    <name>A0A4W2IBF3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP binding cassette subfamily D member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ABCD4</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408216.1">
        <property type="nucleotide sequence ID" value="XM_027552415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IBF3"/>
    <dbReference type="SMR" id="A0A4W2IBF3"/>
    <dbReference type="STRING" id="30522.A0A4W2IBF3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049148.1">
        <property type="protein sequence ID" value="ENSBIXP00000039413.1"/>
        <property type="gene ID" value="ENSBIXG00000023349.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038173.1">
        <property type="protein sequence ID" value="ENSBIXP00005042676.1"/>
        <property type="gene ID" value="ENSBIXG00005026163.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899107"/>
    <dbReference type="CTD" id="5826"/>
    <dbReference type="GeneTree" id="ENSGT00950000182955"/>
    <dbReference type="OMA" id="KQFHDME"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990830">
        <property type="term" value="P:cellular response to leukemia inhibitory factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009235">
        <property type="term" value="P:cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001178">
        <property type="entry name" value="ATP-binding cassette sub-family D member 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="40"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="389"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="606" mass="68838" checksum="F207A74702AE69D3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IK43</accession>
    <name>A0A4W2IK43_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hyaluronan and proteoglycan link protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cartilage-linking protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Proteoglycan link protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HAPLN1</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HAPLN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401473.1">
        <property type="nucleotide sequence ID" value="XM_027545672.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401474.1">
        <property type="nucleotide sequence ID" value="XM_027545673.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401475.1">
        <property type="nucleotide sequence ID" value="XM_027545674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IK43"/>
    <dbReference type="SMR" id="A0A4W2IK43"/>
    <dbReference type="STRING" id="30522.A0A4W2IK43"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042991.1">
        <property type="protein sequence ID" value="ENSBIXP00000043932.1"/>
        <property type="gene ID" value="ENSBIXG00000028119.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041926.1">
        <property type="protein sequence ID" value="ENSBIXP00005044529.1"/>
        <property type="gene ID" value="ENSBIXG00005028378.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895029"/>
    <dbReference type="CTD" id="1404"/>
    <dbReference type="GeneTree" id="ENSGT00940000159267"/>
    <dbReference type="OMA" id="ERACHDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05877">
        <property type="entry name" value="Ig_LP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03518">
        <property type="entry name" value="Link_domain_HAPLN_module_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03519">
        <property type="entry name" value="Link_domain_HAPLN_module_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000631">
        <property type="entry name" value="Hyaluronan and proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000001">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF10">
        <property type="entry name" value="HYALURONAN AND PROTEOGLYCAN LINK PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612139" description="Hyaluronan and proteoglycan link protein 1" evidence="15">
        <location>
            <begin position="16"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="38"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="17">
        <location>
            <begin position="159"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="17">
        <location>
            <begin position="259"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="354" mass="40288" checksum="065D155378A1283C" modified="2019-09-18" version="1">MKSLLLLVLISFCWADHHSDNYTVDHDRVIHIQAENGPRLLVEAEQAKVFSRRGGNVTLPCKFYRDPTAFGSGTHKIRIKWTKLTSDYLKEVDVFVSMGYHKKTYGGYHGRVFLKGGSDNDASLVITDLTLEDYGRYKCEVIEGLEDDTAVVALDLQGVVFPYFPRLGRYNLNFHEAQQACLDQDAVIASFDQLYDAWRSGLDWCNAGWLSDGSVQYPITKPREPCGGQNTVPGVRNYGFWDKDKSRYDVFCFTSNFNGRFYYLIHPTKLTYDEAVQACLNDGAQIAKVGQIFAAWKLLGYDRCDAGWLADGSVRYPISRPRRRCSPSEAAVRFVGFPDKKHKLYGVYCFRAYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IKS7</accession>
    <name>A0A4W2IKS7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Low-density lipoprotein receptor-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">105 kDa low-density lipoprotein receptor-related protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LRP3</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. Its precise role is however unclear, since it does not bind to very low density lipoprotein (VLDL) or to LRPAP1 in vitro.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Binds GGA1 and GGA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <location evidence="13">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371790.1">
        <property type="nucleotide sequence ID" value="XM_027515989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IKS7"/>
    <dbReference type="SMR" id="A0A4W2IKS7"/>
    <dbReference type="STRING" id="30522.A0A4W2IKS7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019824.1">
        <property type="protein sequence ID" value="ENSBIXP00000033424.1"/>
        <property type="gene ID" value="ENSBIXG00000016239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053553.1">
        <property type="protein sequence ID" value="ENSBIXP00005044792.1"/>
        <property type="gene ID" value="ENSBIXG00005004954.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876591"/>
    <dbReference type="CTD" id="4037"/>
    <dbReference type="GeneTree" id="ENSGT00940000160021"/>
    <dbReference type="OMA" id="PTVNIFL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045599">
        <property type="term" value="P:negative regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000050">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000055">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000021">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000031">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000089">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
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</entry>
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</entry>
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</entry>
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    <reference evidence="16 17 18" key="1">
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Lacks formylglycine generating activity and is unable to convert newly synthesized inactive sulfatases to their active form. Inhibits the activation of sulfatases by SUMF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer and heterodimer with SUMF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase-modifying factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383015.1">
        <property type="nucleotide sequence ID" value="XM_027527214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BN57"/>
    <dbReference type="SMR" id="A0A4W2BN57"/>
    <dbReference type="STRING" id="30522.A0A4W2BN57"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014124.1">
        <property type="protein sequence ID" value="ENSBIXP00000001397.1"/>
        <property type="gene ID" value="ENSBIXG00000028613.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053128.1">
        <property type="protein sequence ID" value="ENSBIXP00005025587.1"/>
        <property type="gene ID" value="ENSBIXG00005028083.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883473"/>
    <dbReference type="CTD" id="25870"/>
    <dbReference type="GeneTree" id="ENSGT00940000162897"/>
    <dbReference type="OMA" id="CVRYRAS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1580.10:FF:000002">
        <property type="entry name" value="sulfatase-modifying factor 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="entry name" value="paralog of FGE (formylglycine-generating enzyme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051043">
        <property type="entry name" value="Sulfatase_Mod_Factor_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150:SF33">
        <property type="entry name" value="INACTIVE C-ALPHA-FORMYLGLYCINE-GENERATING ENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150">
        <property type="entry name" value="SULFATASE MODIFYING FACTOR 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610243" description="Inactive C-alpha-formylglycine-generating enzyme 2" evidence="14">
        <location>
            <begin position="26"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase-modifying factor enzyme-like" evidence="15">
        <location>
            <begin position="28"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="301" mass="33829" checksum="3C338435297C6FD5" modified="2019-09-18" version="1">MGISLSPLLTVLSLLSGRWLELGNGQTVNMVQLPGGRFQMGTDSPDGRDGEGPVREVTVKPFAIDIFPVTNKDFREFVREKKYRTEAEVFGWSFVFEDLVSDELRNKATQRMQSLLWWLPVERAFWRQPAGPGSGIREKLEFPVVHVSWNDARAYCAWRGKRLPTEEEWEFAARGGLKGQVYPWGNKFQPNRTNLWQGKFPKGDKAEDGFHGVSPVNAFPPQNDYGLYDLVGNVWEWTASQYQAADQDMRVLRGASWIDTADGSANHRARVTTRMGNTPDSASDNLGFRCASGAGRPPGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQ36</accession>
    <name>A0A4W2BQ36_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin-related family member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Mu-protocadherin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CDHR5</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Intermicrovillar adhesion molecule that forms, via its extracellular domain, calcium-dependent heterophilic complexes with CDHR2 on adjacent microvilli. Thereby, controls the packing of microvilli at the apical membrane of epithelial cells. Through its cytoplasmic domain, interacts with microvillus cytoplasmic proteins to form the intermicrovillar adhesion complex/IMAC. This complex plays a central role in microvilli and epithelial brush border differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5. Interacts (via cytoplasmic domain) with USH1C and MYO7B; required for proper localization of CDHR5 to microvilli tips and its function in brush border differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Microvillus membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQ36"/>
    <dbReference type="STRING" id="30522.A0A4W2BQ36"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012539.1">
        <property type="protein sequence ID" value="ENSBIXP00000002126.1"/>
        <property type="gene ID" value="ENSBIXG00000008631.1"/>
    </dbReference>
    <dbReference type="OMA" id="PDYEANT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000261">
        <property type="entry name" value="Cadherin-related family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF243">
        <property type="entry name" value="CADHERIN-RELATED FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021426819" description="Cadherin-related family member 5" evidence="21">
        <location>
            <begin position="21"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="649"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="118"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="267"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="444"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="704"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="792"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="459"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="504"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="551"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="798"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="826" mass="86148" checksum="5E6D6092145DF590" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQ69</accession>
    <name>A0A4W2BQ69_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4">EZH inhibitory protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQ69"/>
    <dbReference type="STRING" id="30522.A0A4W2BQ69"/>
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        <property type="protein sequence ID" value="ENSBIXP00000001238.1"/>
        <property type="gene ID" value="ENSBIXG00000028779.1"/>
    </dbReference>
    <dbReference type="OMA" id="IRRCTAQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052882">
        <property type="entry name" value="EZH_Inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22467:SF1">
        <property type="entry name" value="EZH INHIBITORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22467">
        <property type="entry name" value="EZH INHIBITORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="446"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="53622" checksum="E34AB941D5FD8A22" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYJ2</accession>
    <name>A0A4W2BYJ2_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Family with sequence similarity 120 member C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAM120C</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the constitutive coactivator of PPAR-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027390484.1">
        <property type="nucleotide sequence ID" value="XM_027534683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYJ2"/>
    <dbReference type="STRING" id="30522.A0A4W2BYJ2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005746.1">
        <property type="protein sequence ID" value="ENSBIXP00000005446.1"/>
        <property type="gene ID" value="ENSBIXG00000000942.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016156.1">
        <property type="protein sequence ID" value="ENSBIXP00005008585.1"/>
        <property type="gene ID" value="ENSBIXG00005013694.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887510"/>
    <dbReference type="CTD" id="54954"/>
    <dbReference type="GeneTree" id="ENSGT00530000063168"/>
    <dbReference type="OMA" id="RYLPMNN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000009">
        <property type="entry name" value="Constitutive coactivator of PPAR-gamma-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026784">
        <property type="entry name" value="Coact_PPARg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15976">
        <property type="entry name" value="CONSTITUTIVE COACTIVATOR OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15976:SF15">
        <property type="entry name" value="CONSTITUTIVE COACTIVATOR OF PPAR-GAMMA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="511"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="932"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="969"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="515"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1010"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1050"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1084"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1094" mass="120145" checksum="17E779A798A49AB3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZ33</accession>
    <name>A0A4W2BZ33_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Antp homeobox family. Proboscipedia subfamily.</text>
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    <dbReference type="SMR" id="A0A4W2BZ33"/>
    <dbReference type="STRING" id="30522.A0A4W2BZ33"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005330.1">
        <property type="protein sequence ID" value="ENSBIXP00000005639.1"/>
        <property type="gene ID" value="ENSBIXG00000011804.1"/>
    </dbReference>
    <dbReference type="OMA" id="DLPHFNF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000145">
        <property type="entry name" value="homeobox protein Hox-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF7">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="138"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="140"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="39"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="192"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
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</entry>
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                <person name="Bickhart D.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <sequence length="738" mass="87189" checksum="48B4A68593095095" modified="2019-09-18" version="1"> </sequence>
</entry>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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</entry>
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    <accession>A0A4W2C1S5</accession>
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    </protein>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006633.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
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    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <dbReference type="Pfam" id="PF23788"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1237" mass="138816" checksum="04D902CA56369124" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
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                <person name="Rosen B.D."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="229" mass="26310" checksum="BE006E822A211C25" modified="2019-09-18" version="1">MAKTQAYFVQFWPQVLQCFAGLYHRLQKCWSVVKGFCSNNEEEYIPPAESIFHKEKILTLGNILTDNSLALEQRAQAAYRIGLLAFTGGPTAGNFAAEYMKEVAHLLQNYEMVPKIKILLLQSVASWCYLNPVNQRRAKHLQFIPILIDLFNDTSESTMTSETNSSLLVKFWACYVLSVMTCNNLPCMQELKHCSSLKYHLQILASENWSGWPENFAEVLYFLIGFHRH</sequence>
</entry>
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    <accession>A0A4W2C9B2</accession>
    <name>A0A4W2C9B2_BOBOX</name>
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            <fullName evidence="5">N-acetylaspartylglutamate synthase</fullName>
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        </recommendedName>
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            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-L-aspartate + L-glutamate + ATP = N-acetyl-L-aspartyl-L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40035"/>
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            <dbReference type="ChEBI" id="CHEBI:16953"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:76931"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RimK family.</text>
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    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072590">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="beta-citrylglutamate synthase B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000022">
        <property type="entry name" value="beta-citrylglutamate synthase B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000014">
        <property type="entry name" value="beta-citrylglutamate synthase B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF5">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="119"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="386" mass="42519" checksum="6E4A2B853B04614D" modified="2019-09-18" version="1">MCSSVAAKLWFLTDRRIREDYPQKEILRALKAKCCEEELDFRAVVMDELVLTVEQGNLGLRINGELITAYPQVVVVRVPTPWVQSDSDITVLRHLEKMGCRLMNRPQAILNCVNKFWTFQELAGHGVPLPDTFSYGGHENFAKMIDEAEVLEFPMVVKNTRGHRGKAVFLARDKHHLADLSHLIRHEAPYLFQKYVKESHGRDVRVIVVGGRVVGTMLRCSTDGRMQSNCSLGGVGMMCSLSEQGKQLAIQVSNILGMDVCGIDLLMKDDGSFCVCEANANVGFIAFDKACNLDVAGIIADYAASLLPSGRLTRRMSLLSVVSTASETSEPELGPPANTAVDNMSASSSSVDSDPETTERELLTKLPGGLFNMNQLLANEIKLLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA12</accession>
    <name>A0A4W2CA12_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spermatogenesis-associated protein 2 PUB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CA12"/>
    <dbReference type="STRING" id="30522.A0A4W2CA12"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044276.1">
        <property type="protein sequence ID" value="ENSBIXP00000009718.1"/>
        <property type="gene ID" value="ENSBIXG00000015350.1"/>
    </dbReference>
    <dbReference type="OMA" id="EPPSAHY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048839">
        <property type="entry name" value="SPATA2_PUB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326:SF9">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2/TAMOZHENNIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21388">
        <property type="entry name" value="SPATA2_PUB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 2 PUB-like" evidence="3">
        <location>
            <begin position="80"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="478" mass="51762" checksum="FBCC5DA994C06A4C" modified="2019-09-18" version="1">MSWPRAPGPKEDTPETLLEDLISFYLEGAGEGRLRVCRQATLTSRAQQLLDTGPPLQLYLPEEVAPDSGAPCFAQHIDHKLVRALEFLELVSIHLLLFPWRKEIRSLKTYTGSFAYWVRPVLSEHTLHTLLGRLGYMATSEVEFSLVQAISEEDTKQMVFEIFLTRVACEAVLRTPGRQLLGPGRERVAGPHHRPGSEMGLQEALWEAQPGLDPSAGVGAKSALAEHPDARCSLPVALNQPEVSTAPHGLLTGPLFPLGSLRHASTCSDSEEFLTCYSDLALHRTPLLPWDQPWSSLEGKQLQGPGPGPSPAPGEVAAASGSSGEQPWVPDGASECKGATVPSQLLQRPRSCLSENSLAPKPDALPDPAALGMDTEPPSTSSEMEELCEHFTHLLRTSTPAGYLRDTPSPRVEEEGQSEPLVGPEPASKGGSPDGKVALLWRSPQASTSWTRAPSAYYVPLKGLEEPVPTRRSYTSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBU9</accession>
    <name>A0A4W2CBU9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adhesion G-protein coupled receptor G1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">G-protein coupled receptor 56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">ADGRG1</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="24">Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1-CT) predominantly remain associated and non-covalently linked. ADGRG1 NT self-associates in a trans-trans manner; the homophilic interaction enhances receptor signaling. Interacts with TGM2. Interacts with heparin; leading to the reduction of ADGRG1 shedding. Interacts with COL3A1. Part of a GPCR-tetraspanin complex at least consisting of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of ADGRG1 with GNA11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBU9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017672.1">
        <property type="protein sequence ID" value="ENSBIXP00000009402.1"/>
        <property type="gene ID" value="ENSBIXG00000006936.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090330">
        <property type="term" value="P:regulation of platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160221">
        <property type="term" value="P:Rho-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000117">
        <property type="entry name" value="adhesion G-protein coupled receptor G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000014">
        <property type="entry name" value="Adhesion G-protein coupled receptor G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040950">
        <property type="entry name" value="ADGRG1_GAIN_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003910">
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        <property type="match status" value="1"/>
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        <location>
            <begin position="1"/>
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            <end position="691"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010933"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00019701"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="691" mass="77418" checksum="FC04FEE9157B2E2D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CE61</accession>
    <name>A0A4W2CE61_BOBOX</name>
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        <recommendedName>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Promotes cell proliferation.</text>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSBIXP00005033342.1"/>
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    </dbReference>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011199.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="260" mass="29521" checksum="C57B8957CBE98422" modified="2019-09-18" version="1">MEEVREGPELGGGMEEPASPQEPASPPPPPSPPSPAAPETPRLPAPESPTEAHARQLLLQEWAPPRGSLELPPRLTWKLLFLRRPLYRNLLRSPNPEGINIYEPAPPTGPTQQPLEALGNFRGWHIRTEKLQQNLSWTVKQQCVDLLAEGLWEELLDDEQPRITVMDWFEDSRLDACVYELHVWLLAADRSTVIAQHHVAPRTSGRGPPGHWIQVSHVFRQYGPGVRFVHFLHKTKNRMERGGLRRTRVTDSSVSVQFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CF43</accession>
    <name>A0A4W2CF43_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Succinate dehydrogenase complex assembly factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SDHAF1</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I LYR family. SDHAF1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CF43"/>
    <dbReference type="SMR" id="A0A4W2CF43"/>
    <dbReference type="STRING" id="30522.A0A4W2CF43"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019456.1">
        <property type="protein sequence ID" value="ENSBIXP00000010576.1"/>
        <property type="gene ID" value="ENSBIXG00000002301.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009108.1">
        <property type="protein sequence ID" value="ENSBIXP00005004049.1"/>
        <property type="gene ID" value="ENSBIXG00005010034.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157289"/>
    <dbReference type="OMA" id="MGTFVRP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20268">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045295">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052687">
        <property type="entry name" value="SDHAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47046">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47046:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="6">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="118" mass="13253" checksum="CA5D10FC5D676AA1" modified="2019-09-18" version="1">MSRHSRLQRQVLSLYRELLRAGRGKPGAEARVRAEFRQHACLPRSDVLRIEYLYRRGRRQLQMLRSGHATAMGAFVRTRGPTEESNGAGAPGTLSGEGDDPRKPLDSMRTPKTPLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGE9</accession>
    <name>A0A4W2CGE9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin-6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CDH6</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGE9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044905.1">
        <property type="protein sequence ID" value="ENSBIXP00000010987.1"/>
        <property type="gene ID" value="ENSBIXG00000016363.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000017">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000006">
        <property type="entry name" value="Cadherin-9 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF322">
        <property type="entry name" value="CADHERIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 15 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021343101" description="Cadherin-6" evidence="18">
        <location>
            <begin position="22"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="79"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="160"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="213"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="328"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="432"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="734" mass="82149" checksum="5FA585889CE460CB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHA9</accession>
    <name>A0A4W2CHA9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ATP6V0A1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR. Interacts with SPAAR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Clathrin-coated vesicle membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372711.1">
        <property type="nucleotide sequence ID" value="XM_027516910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHA9"/>
    <dbReference type="STRING" id="30522.A0A4W2CHA9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044951.1">
        <property type="protein sequence ID" value="ENSBIXP00000011102.1"/>
        <property type="gene ID" value="ENSBIXG00000002247.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043431.1">
        <property type="protein sequence ID" value="ENSBIXP00005026909.1"/>
        <property type="gene ID" value="ENSBIXG00005001971.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877114"/>
    <dbReference type="CTD" id="535"/>
    <dbReference type="GeneTree" id="ENSGT00950000182881"/>
    <dbReference type="OMA" id="FYLWFFL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097401">
        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF68">
        <property type="entry name" value="V-TYPE PROTON ATPASE 116 KDA SUBUNIT A 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="549"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="576"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="641"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="772"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="839" mass="96503" checksum="62F2B49ECF649F4C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ18</accession>
    <name>A0A4W2CJ18_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000021545.1">
        <property type="protein sequence ID" value="ENSBIXP00000011920.1"/>
        <property type="gene ID" value="ENSBIXG00000017083.1"/>
    </dbReference>
    <dbReference type="OMA" id="TQCENRE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000965">
        <property type="entry name" value="Neurotrophin receptor-interacting factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001530">
        <property type="entry name" value="Zinc finger protein 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000204">
        <property type="entry name" value="Zinc finger protein 331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000663">
        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000052">
        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="43"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="564"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="676"/>
            <end position="703"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="1372" mass="156665" checksum="0FECC704E41FE111" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK00</accession>
    <name>A0A4W2CK00_BOBOX</name>
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        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <sequence length="500" mass="51683" checksum="AFFAEBBFE584A553" modified="2019-09-18" version="1">MGASGPRRGPGPPDGGWGWAVLGACFVITGFAYGFPKAVSVFFRALMRDFGAGYSDTAWVSSIMLAMLYGTGPVSSILVTRFGCRPVMLVGGLLASAGMILASFATRLLELYLTAGVLTGLGLALNFQPSLIMLGLYFERRRPLANGLAAAGSPVFLSALSPLGQQLLEHFGWRGGFLLLGGLLLNCCACGAVMRPPPGPGPRPRRDSAGDAPGEAEAHLAGPRVREAPSGGRTRRRLLDVTVCADRAFAVYAVTKFLMALGLFVPAILLVNYAKDAGVPDSEAAFLLSIVGFVDIVARPACGALAGLARLRPHVAYLFSLALMANGLTDLSSARARSYSALVAFCIAFGLSYGMVGALQFEVLMAAVGAPRFPSALGLVLLLEAVAVLIGPPSAGRLVDALKNYEIIFYLAGSEVALAGIFMAVATNCCLRRPRDGPPSPGTEGGAGDGEEAEAEVDSDLPPAGTEEPGGLEAVEVPSPGTGPSQPEVKMEPGLALESV</sequence>
</entry>
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    <name>A0A4W2CMZ1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4">Mitochondrial ribosomal protein L53</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7894" checksum="8821171081CB4EB2" modified="2019-09-18" version="1">MAAALARLGLRSVKQVRVQFCPFEKNVESTRRWASLDYARRSPDRPGNAQCFRLPHPGQGRGGERGQARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CN80</accession>
    <name>A0A4W2CN80_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113906038</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CN80"/>
    <dbReference type="STRING" id="30522.A0A4W2CN80"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023827.1">
        <property type="protein sequence ID" value="ENSBIXP00000013422.1"/>
        <property type="gene ID" value="ENSBIXG00000018355.1"/>
    </dbReference>
    <dbReference type="OMA" id="GVCIFHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="49"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="316" mass="35503" checksum="628CC2AA0A2E219C" modified="2019-09-18" version="1">MALNHEPGVTMKNTTEVTIFVLKGLTDNPEIQFILFFLFLAMYLFTLIGNLGLVVLVIGDCRLHNPMYYFLSVLSSVDACYSSVITPKMLADFTSKNKTILLPECAAQMFLFTTFGTTECFLLAAMAYDRYVAIYNPLLYSASMAPRLYVPFIIASYVGGIVHATVHTVATFSLSFCASNEIRHVFCDIPPLLAISCSDTHTNQLLLFYLVGSIEIVTVLIVLISYSFILLAVLRMHSAEGRRKVFSTCGSHLTGVCIFHGTILFMYVRPSSSYALDHDMIVSLFYSVVIPMLNPIIYSLRNKDVKEAMKRMFWRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNB6</accession>
    <name>A0A4W2CNB6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotrimer. Heterotrimer; with other ASIC proteins producing functional channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC4 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CNB6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025628.1">
        <property type="protein sequence ID" value="ENSBIXP00000014650.1"/>
        <property type="gene ID" value="ENSBIXG00000019282.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.470.10:FF:000001">
        <property type="entry name" value="Acid-sensing (proton-gated) ion channel family member 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000001">
        <property type="entry name" value="Acid-sensing ion channel subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF13">
        <property type="entry name" value="ACID-SENSING ION CHANNEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 17" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="12 17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="493"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="544" mass="59547" checksum="551000F4F20D975D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQP9</accession>
    <name>A0A4W2CQP9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">L3MBTL histone methyl-lysine binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">L3MBTL3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031503"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015659.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="698" mass="79954" checksum="491613D115A36013" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUX5</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="25">Serine/threonine-protein kinase H1</fullName>
            <ecNumber evidence="8">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Protein serine kinase H1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PSKH1</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_027372008.1">
        <property type="nucleotide sequence ID" value="XM_027516207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUX5"/>
    <dbReference type="SMR" id="A0A4W2CUX5"/>
    <dbReference type="STRING" id="30522.A0A4W2CUX5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044461.1">
        <property type="protein sequence ID" value="ENSBIXP00000010097.1"/>
        <property type="gene ID" value="ENSBIXG00000015671.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050058.1">
        <property type="protein sequence ID" value="ENSBIXP00005040442.1"/>
        <property type="gene ID" value="ENSBIXG00005006519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876705"/>
    <dbReference type="CTD" id="5681"/>
    <dbReference type="GeneTree" id="ENSGT00940000157041"/>
    <dbReference type="OMA" id="SYAGEHW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14087">
        <property type="entry name" value="STKc_PSKH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000416">
        <property type="entry name" value="Serine/threonine-protein kinase H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 27 28" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="24" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0519">Myristate</keyword>
    <keyword evidence="15 27 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 28" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="98"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="378"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00072468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="424" mass="48009" checksum="83C6943A3BBDCD2F" modified="2019-09-18" version="1">MGCGTSKVLPEPPKDVQLDLVKKVEPFSGTKSDVYKHFITEVDSVGPLKGGFPAASQGANPSPGTPRTSHTEPPSEPPRRARVAKYRAKFDPRVTAKYDIKALIGRGSFSRVVRVEHRATRQPYAIKMIETKYREGREVCESELRVLRRVRHANIIQLVEVFETQERVYMVMELATGGELFDRIIAKGSFTERDATRVLQMVLDGVRYLHALGITHRDLKPENLLYYHPGTDSKIIITDFGLASARKKGDDCLMKTTCGTPEYIAPEVLVRKPYTNSVDMWALGVIAYILLSGTMPFEDDNRTRLYRQILRGKYSYSGEPWPSVSNLAKDFIDRLLTVDPGARMTALQALRHPWVVSMAASSSMKNLHRSISQNLLKRASSRCQSTKSAQSTRSSRSTRSNKSRRVRERELRELNLRYQQQYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYR5</accession>
    <name>A0A4W2CYR5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYR5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020880.1">
        <property type="protein sequence ID" value="ENSBIXP00000011493.1"/>
        <property type="gene ID" value="ENSBIXG00000016715.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20669">
        <property type="entry name" value="Cyp2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000001">
        <property type="entry name" value="Cytochrome P450, family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_CYP2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF275">
        <property type="entry name" value="CYTOCHROME P450 2F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01957">
        <property type="entry name" value="EP450ICYP2F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021489971" evidence="15">
        <location>
            <begin position="25"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="491" mass="55901" checksum="4A91B07D972405A1" modified="2019-09-18" version="1">MDSISTAILLLILALVCLLLTTSSKGKGRLPPGPRALPFLGNLLQLRSQDMLTSLTKLSKEFGAVYTVYLGPRRVVVLSGYQAVKEALVDQAEEFSGRGNYPVFFNFTKGNGIAFSNGDRWKVLRKYSVQILRNFGMGKRTIEERILEEGHFLLEELRKTQGKPFDPTFVVSRSVSNIICSVIFGSRFDYDDDRLLTIIHLINENFQIMSSPWGEMYNMFPNLLDWVPGPHRRLFKNYGRIKDIIARSVREHQASLDPNSPRDFIDCFLTKMAQEKQDPLSHFFMDTLLMTTHNLLFGGTETVGTTLRHAFRLLMKYPEVQVRVQEEIDRVVGRERLPTVEDRAAMPYTDAVIHEVQRFADVIPMSLPHRVIRDTNFRGFTIPRGTDVITLLNTVHYDPSQFLKPKEFNPEHFLDANMSFKKSPAFMPFSAGRRLCLGEALARMELFLYLTAILQSFSLQPLGAPEDIDLTPLSSGLGNLPRPYQLCVLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D168</accession>
    <name>A0A4W2D168_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D168"/>
    <dbReference type="SMR" id="A0A4W2D168"/>
    <dbReference type="STRING" id="30522.A0A4W2D168"/>
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        <property type="protein sequence ID" value="ENSBIXP00000012382.1"/>
        <property type="gene ID" value="ENSBIXG00000017476.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032885.1">
        <property type="protein sequence ID" value="ENSBIXP00005019816.1"/>
        <property type="gene ID" value="ENSBIXG00005023000.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000195352"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
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        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="117" mass="13407" checksum="45C69CB3BB169CEE" modified="2019-09-18" version="1">MTTSPTFSQCTGQVKTRLVEGLCRRSHSIEVHPQNVCWAGTPPLGPCCFLLTRQLYDDYIEEFDCPVLDEERTVDKLENQMSEGGGLVDYYGCDFFSELWLHIVFVLKTDKCFVQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1A9</accession>
    <name>A0A4W2D1A9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1A9"/>
    <dbReference type="STRING" id="30522.A0A4W2D1A9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030774.1">
        <property type="protein sequence ID" value="ENSBIXP00000017993.1"/>
        <property type="gene ID" value="ENSBIXG00000021894.1"/>
    </dbReference>
    <dbReference type="OMA" id="GFMCHVT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48424">
        <property type="entry name" value="DYNEIN LIGHT CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48424:SF3">
        <property type="entry name" value="DYNEIN LIGHT CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="119" mass="13337" checksum="A61A5902753E1EB1" modified="2019-09-18" version="1">MLLTRFEQENGHLAQVEVDEVFGFMCHITTEVPPHDAVPGGVVLLIKLLLDMGRNVLLYIILFQCLSSTLHRVLLHLLRHIGILDYGLSVIHGYQGAGADAWMASPIQWTMVWVDSGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1C3</accession>
    <name>A0A4W2D1C3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Glutamate decarboxylase 2</fullName>
            <ecNumber evidence="7">4.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">65 kDa glutamic acid decarboxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Glutamate decarboxylase 65 kDa isoform</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">GAD2</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the production of GABA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27 28">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Presynaptic cell membrane</location>
            <topology evidence="23">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 28">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1C3"/>
    <dbReference type="STRING" id="30522.A0A4W2D1C3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045588.1">
        <property type="protein sequence ID" value="ENSBIXP00000012442.1"/>
        <property type="gene ID" value="ENSBIXG00000017522.1"/>
    </dbReference>
    <dbReference type="OMA" id="AGMVIFK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012442.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="585" mass="65413" checksum="7DE4F9A5EA62D2A6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1K9</accession>
    <name>A0A4W2D1K9_BOBOX</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">LOC113899444</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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            <topology evidence="12">Multi-pass membrane protein</topology>
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        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_4"/>
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    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="47"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="98"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="228"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="308" mass="34720" checksum="88D715235C563E50" modified="2019-09-18" version="1">MNETNHSQVTEFVLLGLSNSQELQPFLFLIFSLLYLAILLGNFLIILTVTSDFRLHTPMYFLLANLSFIDICVASFATPKMIADFLVQRKTISFDACLAQIFFVHLFTGSEMVILVSMAYDRYVAICRPLHYMTIMSHRVCIILVLISWCVGFIHTTSQLAFTVNLPFCGPNQVDSFFCDLPLVTKLACIDTYVVSLLIVADSGFLSLSSFLLLVLSYTVILVTVRSHSSASMAKARSTLTAHITVVTLFFGPCIFIYVWPFSSYSIDKVLAVFYTIFTPILNPVIYTLRNKEMKAAMSKLKSRYLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D259</accession>
    <name>A0A4W2D259_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">25/26-hydroxycholesterol 7alpha-hydroxylase</fullName>
            <ecNumber evidence="18">1.14.14.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Oxysterol 7-alpha-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC113904213</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 20 21">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; bile acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 20">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.29" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D259"/>
    <dbReference type="STRING" id="30522.A0A4W2D259"/>
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        <property type="protein sequence ID" value="ENSBIXP00000012740.1"/>
        <property type="gene ID" value="ENSBIXG00000017801.1"/>
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    <dbReference type="OMA" id="WSEVVQM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0033783">
        <property type="term" value="F:25-hydroxycholesterol 7-alpha-hydroxylase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:iron ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:oxysterol 7-alpha-hydroxylase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:bile acid biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cholesterol metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450 family 7 subfamily B member 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF0">
        <property type="entry name" value="CYTOCHROME P450 7B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="9 20" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 20 21" id="KW-0349">Heme</keyword>
    <keyword evidence="12 20 21" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 20 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0492">Microsome</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077814"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="510" mass="58915" checksum="65835500C43841F7" modified="2019-09-18" version="1">TAEDMNAGMGGFWPEPWLTRSLGYPGLALAAALLLLVLCLNARRTRRPGEPPLIKGWLPYFGQALKLQKDPLGFMTSLQKQYGDIFTLLLGGKYITFILDPFHYTSVAKNQKLSFQIFTNKFLKRVFSIKKMLTDSDLIDEIHSTYQFLQGKHLDILMESTMQNLKQVFEPQLLKTTSWSTEYLLPFCNSVIFEMTFTTIYGNILADDKKTFITELKDDFLKFDEKFTRLASGIPIELLGNIKSVRTKLIKDLTIESLAKLQGMSEVVQRRNDILEKYYTPKDTEIGAHHLGLLWASVTNTVPTMFWAMYYLLRNPKAMAVLRDEIDHLLQSTGQKKGPGFSIYLTREQLDSLVYLESTILEVLRLCSFSGIFRFVQEDLTLHLESQDCCLRKGDFVVIFPPILHHDPEIFEAPDEFRFDRFTENGKKKTTFFKRGKKLKYYHLPFGLGVSKCPGRFLAMVEIKQLLVVLLTYFDLEIIDNKPLELNYSRFLFGIPYPDSDVLFRYKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2S8</accession>
    <name>A0A4W2D2S8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2S8"/>
    <dbReference type="STRING" id="30522.A0A4W2D2S8"/>
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        <property type="gene ID" value="ENSBIXG00000004656.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5021453736" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
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            <end position="124"/>
        </location>
    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="127" mass="14079" checksum="96533791231A3B76" modified="2019-09-18" version="1">MGNQVICCVALCLFGAGTVAGEVTQTPKYLLKKEGGALTLECEQDFDYDSMYWYRQDPGLGLRLIFFSRVVNTVQKEDIAKGYSASRENKPFFPLTVTSAQKNQTALYLCAASRDTVTQSHLLSAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D452</accession>
    <name>A0A4W2D452_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Zinc finger protein 134</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D452"/>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="466" mass="53129" checksum="1C7CD12F86EB859A" modified="2019-09-18" version="1">MAAAALRGPPQACVTFEDVAIYFSQDEWRLLNETQRFLYCDVMLENFALTTSLGCWHGVDKEEAASEQSVSVAVSHIHASRADSLTQMAHPCTLCGPTLKDVLHLDEHRETRHGLKPYTCGACGRQFWFSANFDQPQKLYDVEKLLRGDKGKTSFVTNYRACEEPLLSEKPFAYKEEQKKFQASLGSHQQKATPSNRKTRSPESGLALHIGQMHYKCIECGKAFSRKDTLVQHQRIHTGEKPYECSECGKAFSRKATLVQHQRIHTGEKPYECSECGKAFSRKDNLTQHKRVHTGEMPYKCGECGKYFSHHSNLIVHQRVHNGARPYKCNDCGKVFRHKSTLVQHESIHTGENPYVCSDCGKSFGHKYTLIKHQRIHTEARPFECIECGKFFSRSSDFIAHQRVHTGERPFVCSKCGKDFIRTSHLVRHQKVHTGERPYECNECGKSYSLSSHLIRHQKVHAAGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D557</accession>
    <name>A0A4W2D557_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="15">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
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            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with WIP and actin. Interacts with TBC1D23.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D557"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000032890.1">
        <property type="protein sequence ID" value="ENSBIXP00000019344.1"/>
        <property type="gene ID" value="ENSBIXG00000022976.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FKBP-15_dom"/>
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    </dbReference>
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        <property type="entry name" value="PPIase_FKBP_dom"/>
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    <dbReference type="PANTHER" id="PTHR44927">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="18">
        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="294"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="921"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="567"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="663"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="814"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="929"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1024"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1045"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1104"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1171"/>
            <end position="1183"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1183" mass="129611" checksum="4FE79BE6AEA217FF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6N2</accession>
    <name>A0A4W2D6N2_BOBOX</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2D6N2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033731.1">
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        <property type="gene ID" value="ENSBIXG00000023365.1"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="140" mass="15404" checksum="AF06DB991D93432D" modified="2019-09-18" version="1">YWGRLCLWPSLWNPLGTFQSFISKESPQGSGQVLVKVMECLEHQALTDLGSHEAYGTTGESSCFCGLAPPLDMNRMEPMWSILEDKCLHLRSNSVEDIQRATTNLPLSYGGVFCASTHTPIPPSASLCQKMDEQEPVSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8I7</accession>
    <name>A0A4W2D8I7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3">NADP-retinol dehydrogenase</fullName>
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        </recommendedName>
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    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; retinol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A4W2D8I7"/>
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        <property type="protein sequence ID" value="ENSBIXP00000020588.1"/>
        <property type="gene ID" value="ENSBIXG00000023887.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="Retinol dehydrogenase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF70">
        <property type="entry name" value="RETINOL DEHYDROGENASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="adh_short"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="20"/>
            <end position="340"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="340" mass="37807" checksum="B37D6A4430A1D5CC" modified="2019-09-18" version="1">MVGVLLLLLLLPFLLYIAAPQIRKMLSSGVCTSTIQLPGKVAVVTGANAGIGKETAKELARRGARVYLACRDVQNGELVAREIQMMTGNQQVLVRKLDLADTKSIRAFAKRFLEEEKHLHILINNAGVMMCPYSKTADGFEMHMGVNHLGHFLLTHLLLEKLEESAPSRVVNVSSLAHLLGRIHFHNLQGEKFYQSGLAYCHSKLANILFTQELARRLKASGSGVTVYSVHPGTVNSELVRHSALMRWIWWIFSFFIKTPQQGAQTSLYCALTEGLEVLSGNHFSDCHVAWVSAQARNETVARRLWDVSCDLLGIPVDWLMMAAGPKRSLKQTTQHTLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8T7</accession>
    <name>A0A4W2D8T7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8T7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034904.1">
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        <property type="gene ID" value="ENSBIXG00000023961.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="367" mass="42475" checksum="51F633FA36701AA7" modified="2019-09-18" version="1">MGSIKDRNGIDLTEAEDIKKRWQEYTEELYKKELHNQDNHDGVITHLEPDILEREVKWALERITTNKASGGDGIPVELFQILKDDAVKVLHAICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASQVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANIHWIIEKARQFQKSIYFCFIHYTKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQKAIVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNTGLEEAQAGIKTAGRNINNLRYADDTTLMAESEEELLKSLLMKVKEESEKVGLNLNIQKTKVMASGPITLWVINGETVETASDFFWLQNHYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBE9</accession>
    <name>A0A4W2DBE9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family. USP17 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBE9"/>
    <dbReference type="STRING" id="30522.A0A4W2DBE9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050050.1">
        <property type="protein sequence ID" value="ENSBIXP00000021593.1"/>
        <property type="gene ID" value="ENSBIXG00000024638.1"/>
    </dbReference>
    <dbReference type="OMA" id="GENDYHC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000070">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 17-like protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF651">
        <property type="entry name" value="INACTIVE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 17-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="77"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="503" mass="54349" checksum="4D4446A53194252D" modified="2019-09-18" version="1">QPMRSLHVRGWGCGEAPKGPRGRGPGPFLEPDRLREGGGPSPALGRPQRGDLAPGSAGLTPGQKGALSWKGPWGVGAGLQNLGNTCYVNAALQCLSHTPPLASWMVSQQHATLCPARSACTLCAMRAHVTRALLHAGEVIRPRKDLLAGFHRHQQEDAHEFLMFTLNAMQQGCLSASQPSGHASEDTTVIRQIFGGTWRSQIQCLRCLGVSDTFDPYLDISLDITAAQSVEQALRELVKPEKLDADNAYDCGVCLRKVPATKRLTLHSTSQVLVLVLKRFTPVSGAKRAQEVRYPQCLDLQPYTSERKAGPLGYVLYAVLVHSGWSCERGHYFCYVRAGNGQWYKMDDAKVTACDETAALSQSAYVLFYAREGAWEGGAGGGAAAPVGADPTDPGSLQETPVEGMSLEQWRRLQEHSRPKPALELRKVQSALPAGAVVIHQSKHGGGRNRTPPQQEHERLDRPSTDTPPPGPKNVGNGPCASGRARATKGKNKKPRPSLGLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DC23</accession>
    <name>A0A4W2DC23_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DC23"/>
    <dbReference type="STRING" id="30522.A0A4W2DC23"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039215.1">
        <property type="protein sequence ID" value="ENSBIXP00000023576.1"/>
        <property type="gene ID" value="ENSBIXG00000005353.1"/>
    </dbReference>
    <dbReference type="OMA" id="MKFPIMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027895">
        <property type="entry name" value="DUF4533"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36289">
        <property type="entry name" value="CHROMOSOME 12 OPEN READING FRAME 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36289:SF1">
        <property type="entry name" value="CHROMOSOME 12 OPEN READING FRAME 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15047">
        <property type="entry name" value="DUF4533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="238" mass="26889" checksum="8E3CB84E6AFC38AB" modified="2019-09-18" version="1">MSSESEKDKERFIQAAKTFFFHMQDLASFTNALTKLFNSSMSTQILLMTVKEDGNVKNVFEQMLRIFKEMQSVVAAKQDPMQSEPLSSKIATAMFSMVEKSNNIRELQQSTKEMFKNVQSPIIASVLNSGNILESLESSLLLLMKYPIMNLQLSDFYRKEQSDATTSEKSPGPSKATTIDALKKLQDVLNIENAKYTIKSAADQMEQIVKTMGPILEVLQKAIKAMETKFSVFKKPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCI6</accession>
    <name>A0A4W2DCI6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DAZ-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deleted in azoospermia-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DAZAP1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">RNA-binding protein, which may be required during spermatogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with DAZ and DAZL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027402392.1">
        <property type="nucleotide sequence ID" value="XM_027546591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCI6"/>
    <dbReference type="SMR" id="A0A4W2DCI6"/>
    <dbReference type="STRING" id="30522.A0A4W2DCI6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036946.1">
        <property type="protein sequence ID" value="ENSBIXP00000022011.1"/>
        <property type="gene ID" value="ENSBIXG00000005094.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895410"/>
    <dbReference type="CTD" id="26528"/>
    <dbReference type="OMA" id="FVMFRKA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12574">
        <property type="entry name" value="RRM1_DAZAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12327">
        <property type="entry name" value="RRM2_DAZAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000129">
        <property type="entry name" value="DAZ-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000093">
        <property type="entry name" value="Putative DAZ-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034134">
        <property type="entry name" value="DAZAP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034131">
        <property type="entry name" value="DAZAP1_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF18">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="113"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="74"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="185"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="363"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="406" mass="43356" checksum="01298497031B597D" modified="2019-09-18" version="1">MNNSGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSTPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPATPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYSQYGYGQDLASFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFK2</accession>
    <name>A0A4W2DFK2_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Small transmembrane regulator of ion transport 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">STRIT1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFK2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000022932.1"/>
        <property type="gene ID" value="ENSBIXG00000002079.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007063.1">
        <property type="protein sequence ID" value="ENSBIXP00005030827.1"/>
        <property type="gene ID" value="ENSBIXG00005010857.1"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="P:regulation of slow-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20247">
        <property type="entry name" value="DWORF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044529">
        <property type="entry name" value="DWORF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22030">
        <property type="entry name" value="DWORF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="35" mass="3885" checksum="5ED5D1F1CD69C5F9" modified="2019-09-18" version="1">MAEKAEATLSRYVVPILLLIGWIVGCIVMVYVVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGR1</accession>
    <name>A0A4W2DGR1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGR1"/>
    <dbReference type="STRING" id="30522.A0A4W2DGR1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051027.1">
        <property type="protein sequence ID" value="ENSBIXP00000023529.1"/>
        <property type="gene ID" value="ENSBIXG00000001816.1"/>
    </dbReference>
    <dbReference type="OMA" id="MKCSLMQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="88" mass="10327" checksum="7BC7D74CE919A7CB" modified="2019-09-18" version="1">MKVRHFNGFLKLMKCSLMQRKGNHTTKEENRQLKTVEQVVVWAPPRTSLICFSEEETGCREREGGKNVVTMLFLLNKEKTFSCVWIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DH03</accession>
    <name>A0A4W2DH03_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SLX9 ribosome biosis factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SLX9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DH03"/>
    <dbReference type="STRING" id="30522.A0A4W2DH03"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052255.1">
        <property type="protein sequence ID" value="ENSBIXP00000025517.1"/>
        <property type="gene ID" value="ENSBIXG00000027599.1"/>
    </dbReference>
    <dbReference type="OMA" id="GAKEWAF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109">
        <property type="entry name" value="PROTEIN FAM207A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN SLX9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="219" mass="24081" checksum="16327407154F7509" modified="2019-09-18" version="1">MGKVRSLRARVHQVAVRPTGEAAPGLASPAREVAPLQASTGGVAGKELACLNADVFAGTTIDPSALVQQLDVETRSVTSIRRGAEAKTVLPKKEKLKLRRERWLQKIEAIKLAEQKLKAERRRRATVVVGDLQPLRDALPELLELEAGGRRPPTRRRAASRPRPAELSRMSAMQRLQLLEEERTRFQELLASPAYRASPLLAIGRQLAEQMQLEGGGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIU3</accession>
    <name>A0A4W2DIU3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Multiple epidermal growth factor-like domains protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Epidermal growth factor-like protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MEGF9</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIU3"/>
    <dbReference type="SMR" id="A0A4W2DIU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048638.1">
        <property type="protein sequence ID" value="ENSBIXP00000018784.1"/>
        <property type="gene ID" value="ENSBIXG00000022559.1"/>
    </dbReference>
    <dbReference type="OMA" id="GTCHTDS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:tissue development"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Laminin subunit gamma 2"/>
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        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 9"/>
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        <location>
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        </location>
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            <begin position="35"/>
            <end position="612"/>
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        <location>
            <begin position="525"/>
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        <location>
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            <begin position="29"/>
            <end position="206"/>
        </location>
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        <location>
            <begin position="85"/>
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            <begin position="105"/>
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            <begin position="137"/>
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            <begin position="196"/>
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        <source>
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        </source>
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    <sequence length="612" mass="63380" checksum="6F3F8FCA386CF68D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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            <taxon>Eutheria</taxon>
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            <taxon>Pecora</taxon>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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            <begin position="69"/>
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        </location>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="210" mass="21788" checksum="D94A63EA2F59DBE5" modified="2019-09-18" version="1">MTAPKTCDRARVASTLRYCMMVSGTVVLVAGTLCFAWWSEGDAGPPPGQSAPPTGCPEPGAPGALLRSVSFFCCGAGGLLLLFGLLWSVKASTQKLPRWDPYHLSRDLYYLTVESSEKQSCRTPKVITVPTYEEAVCCPLAGGPLILPASPVEEDLERSASGDALPGAQPPLPPPSYESLIFAAGGISGETARGAACSLTGPVQITEGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DL55</accession>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name evidence="16" type="primary">DDX3X</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000027039.1"/>
        <property type="gene ID" value="ENSBIXG00000028898.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005012392.1"/>
        <property type="gene ID" value="ENSBIXG00005016992.1"/>
    </dbReference>
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    <dbReference type="OMA" id="CYRSWVR"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="DEADc_DDX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000160">
        <property type="entry name" value="ATP-dependent RNA helicase DDX3X"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="645" mass="71309" checksum="CAE5B32349BC4F4D" modified="2019-09-18" version="1"> </sequence>
</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="110" mass="12739" checksum="1E85F08AA481373F" modified="2019-09-18" version="1">MEKRLQEAQLYKEKGNQCYREGKYRDAVSGYHRALLQLRGLDPSLPSPIPNLGPQGLALTPEQENLLHTTQTDCYNNLADANVRRYLQRTQLELSSYHRKEKQLYLGMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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    <reference evidence="7 8 9" key="1">
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                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
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        <property type="term" value="C:chromaffin granule membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:phagocytic vesicle membrane"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:specific granule"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003540"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021850.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="323" mass="36141" checksum="04A4C3562745AF4A" modified="2019-09-18" version="1">MASIWVGKRGTIRDYAGFNPSVDAEAIRKAIRGIGTDEKTLISILTERTNAQRLLIAKEYQALCGKELKDDLKGDLSGHFKHLMVALVTPPAVFDAKQLKKSMKGMGTNEDALIEILTTRTSKQMQEIGHAYYTAYKKSLGDEISSETSGDFRKALLILANGRRDESLKVDEQLARKDAQILYNAGEKRWGTDEDAFTDILCLRSFPQLKLTFDEYRNISQKDIEDSIKGELSGHFEDLLLAIVRCARNTPAFLAERLYRALKGAGTDEFTLNRIMVSRSEIDLLDIRAEFKKLSGYSLYSAIKSDTSGDYEITLLKICGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DU18</accession>
    <name>A0A4W2DU18_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gastric inhibitory polypeptide</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glucose-dependent insulinotropic polypeptide</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glucagon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DU18"/>
    <dbReference type="STRING" id="30522.A0A4W2DU18"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005099.1">
        <property type="protein sequence ID" value="ENSBIXP00000030089.1"/>
        <property type="gene ID" value="ENSBIXG00000011909.1"/>
    </dbReference>
    <dbReference type="OMA" id="GHSRFHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031769">
        <property type="term" value="F:glucagon receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039078">
        <property type="entry name" value="GIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000532">
        <property type="entry name" value="Glucagon_GIP_secretin_VIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15211:SF0">
        <property type="entry name" value="GASTRIC INHIBITORY POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15211">
        <property type="entry name" value="GLUCOSE-DEPENDENT INSULINOTROPIC POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00123">
        <property type="entry name" value="Hormone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00070">
        <property type="entry name" value="GLUCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00260">
        <property type="entry name" value="GLUCAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="9">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="143" mass="16381" checksum="B4676DC26ED91694" modified="2019-09-18" version="1">MVVTIIYCCALCSYGTSVSLSLPSLPLRFHSKASGSQPRATRYAEGTFISDYSIAMDKIRQQDFVNWLLAQKGKKSDWIHNITQREAGALELAHQSNRKEEAKEKQGSLPKNPSDEDLLKHLLIRELLAWMVDQMELCRLRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DU45</accession>
    <name>A0A4W2DU45_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tubulin-folding cofactor E</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DU45"/>
    <dbReference type="STRING" id="30522.A0A4W2DU45"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005010.1">
        <property type="protein sequence ID" value="ENSBIXP00000030119.1"/>
        <property type="gene ID" value="ENSBIXG00000001079.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000008">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000268">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000593">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="507" mass="56619" checksum="457D8327F7734A61" modified="2019-09-18" version="1">MNSTSTSDVIGRRVEVNGEHATVRFSGLVPPVAGLWLGVEWDNPERGKHDGSHEGTVYFKCRHPTAGSFIRPHKVNFGVDFLTAIKNRYVLEDEPKEEETEQIVIIGNKPVETIGFDSVIKQQSQLSKLQDVSLRNCAVNGAGDKGEIAKACPNIRSIDLSKNLLSSWEEVIDIADQLKHLEVLNLSENKLTSPSSSPSPTGTFSTLKLLVLNRTGVTWAEVLRCASGWPVLEKLYLESNNIIISERPTDVLQTVKLLDLSSNQLIDENQLFLIAYLPRLEQLILSDIGISSIHFPDAGIGCKTSMFPSLQYLVLNDNQIAQWSVMNELDKLQSLHALSCTRNPLTEGSKDAQTTRQFIIARIGQLRTLNKCAIEPEERRGAELDYRKAFGNEWKKAGGHQDPEKNRPNEEFLAAHPRYQALCLKYGAPEDGELKTQQPFLLKNQLLTLKIKYPNQHDQKVIEKQLPGKNGCLSPLFLLLNRWPSHEAAGSRTVHSECGLLQLVSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DV53</accession>
    <name>A0A4W2DV53_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inositol-phosphate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DV53"/>
    <dbReference type="STRING" id="30522.A0A4W2DV53"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010535.1">
        <property type="protein sequence ID" value="ENSBIXP00000028363.1"/>
        <property type="gene ID" value="ENSBIXG00000009505.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020552">
        <property type="entry name" value="Inositol_monoPase_Li-sen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF29">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00378">
        <property type="entry name" value="LIIMPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="198"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="411" mass="44154" checksum="12BF8E2898F7DBD1" modified="2019-09-18" version="1">MKASGEDGEAPAGGPWEECFEAAVQLALRAGQIIRKALSEEKRVSTKTSAADLVTETDHVVEALILQELQTRFPSHRFIAEEAAAAGAKCVLTPSPTWIVDPIDGTCNFVHRFPTVAVSIGFAVNQELEFGVIYHCTEERLYTGRRGQGAFCNGQRLRVSGETDLSKALVLTEIGPRRDPATLKLFLSNMERLLHAGAHGYASPPGTSGQAQQPATPWVGAGHTHASQSAARSSTAPGGGRALSSAPSVTSVMPHPLSLPWVCPMTAAAPRGLNPRSSRVRRVKAEFFCCSTSHTSGSATEGWSVSIGGSVGQHRSGLRAQSRDCGKRPWAPVQLLSQETFRGHRWSRAIQCAHPLPLWQGSCHWQLHPGTLPSGCGHRRRLLPVWPALLGPGCSHSHHQGSWGRCDGHVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DX30</accession>
    <name>A0A4W2DX30_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Electron transfer flavoprotein subunit alpha</fullName>
            <shortName evidence="9">Alpha-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ETFA</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.</text>
    </comment>
    <comment type="function">
        <text evidence="9">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 10">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity. Interacts with TASOR.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DX30"/>
    <dbReference type="SMR" id="A0A4W2DX30"/>
    <dbReference type="STRING" id="30522.A0A4W2DX30"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002555.1">
        <property type="protein sequence ID" value="ENSBIXP00000030958.1"/>
        <property type="gene ID" value="ENSBIXG00000001278.1"/>
    </dbReference>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 9 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="11">
        <location>
            <begin position="22"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="248"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="262"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="279"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="333" mass="34957" checksum="93C5E2B20B962001" modified="2019-09-18" version="1">MFRAAAPGQLRRATSLLRFQSTLVIAEHANDTLAPITLNTITAAKHLGGEVSCLVAGTKCDKVAQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILATQKQFNHTHICAGASAFGKNLLPRIAAKLDVAPISDIIAIKSPDTFVRTIYAGNAICTVKCDEKVKVFSVRGTSFEAAAASGGSASSEKASSTSPVGISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDLQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTELLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1Z5</accession>
    <name>A0A4W2E1Z5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSBIXP00000030091.1"/>
        <property type="gene ID" value="ENSBIXG00000011906.1"/>
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    <dbReference type="OMA" id="AVEQQWE"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="502" mass="58421" checksum="DDFAE3B03AB88E78" modified="2019-09-18" version="1">MGEFNSDDHYIYYCRQESLRRNGVAIMVNKRVRNAVLGGNLKNDRMISVHFQGKPFNITVIQVYAPTSNAEEAEVEQFYEDLQNLLELTPKKDVIFIIGDWNAKVGSQETPGVTGTFGFGIRNEGGQGLIEFCQENALVIANTIFQQHKRRLYTWTSPDGQHRNQIDYILCSQRWRSSIQSAKTRSDCGSDHELLIAKFRLKLKKVRKTTRPFRYDLNQIPYDYTVEVRNRFKDLDLIDRLPDELWTEVHDIVQETGIKTIPMEKKCKKAKWLSGEALQIAVKRREEKSKGEKERYKHLNAEFQRIARRDKKAFLSDQCKETEENNRMGKTRDLFKKIRDTKGIFNAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITHLEPDILECEVKWALESITTNKASGDDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTTALISHASKVMLKILQARLQAIHEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E452</accession>
    <name>A0A4W2E452_BOBOX</name>
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        <recommendedName>
            <fullName evidence="5">Scaffolding anchor of CK1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E452"/>
    <dbReference type="STRING" id="30522.A0A4W2E452"/>
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        <property type="protein sequence ID" value="ENSBIXP00000034056.1"/>
        <property type="gene ID" value="ENSBIXG00000016966.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
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    </dbReference>
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        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipase D/nuclease"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <end position="295"/>
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            <end position="379"/>
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            <begin position="320"/>
            <end position="334"/>
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            <begin position="335"/>
            <end position="347"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
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            <end position="361"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="435" mass="47844" checksum="C8DF93513A23A954" modified="2019-09-18" version="1">MSRARHVGKIQKRLEDVKSQWVQPARADFSDNESARLATDALLDGGPEAYWQALSQEGEVDFLSSVEAQYIQAQAKEPPCAPEPPGAAEAGPKGLDTCSLQSGTYFPVASEGSEPALLHTWASAEKPYMKEKSSATVYFQTDKHSNIRDLIRRCITRTSQVLAILMDVFTDVEIFCDILEAANKRGVFVCVLLDQGGVKLFQEMCDKAQISDTHLKNISIRSVEGEVYCAKSGRKFSGQIREKFIISDWRYVLCGSYSFTWLCGHVHRNILSKFTGQPVELFDEEFRHLYASSKPVMGLKSPRLATPLQPGAAPGPPPGRLSDSSGSASDRTSSNPFNSPSTGSNPQTRSLSTSSGPGSPLAPNPLLASRFQPHHSPWGALTPQAHFSPRPHDSLPALYSNLNAYRPTRLQLEHLGLVPRVAHTWRPFLQASPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E596</accession>
    <name>A0A4W2E596_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel subfamily K member 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E596"/>
    <dbReference type="SMR" id="A0A4W2E596"/>
    <dbReference type="STRING" id="30522.A0A4W2E596"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001577.1">
        <property type="protein sequence ID" value="ENSBIXP00000031278.1"/>
        <property type="gene ID" value="ENSBIXG00000001369.1"/>
    </dbReference>
    <dbReference type="OMA" id="LQSDCSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Potassium channel subfamily K member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005407">
        <property type="entry name" value="KCNK9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF75">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01585">
        <property type="entry name" value="TASK3CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="206"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="299"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="503" mass="55154" checksum="4D4586BEECD5774B" modified="2019-09-18" version="1">MALRSGAGCFGRVWRRVSGLPDSWPRRSSSLLCLSAFSPEPGPAPPLPRQPPRGGSGCGGPGGGGPPQPPRPPPPRCCCCCCCCRRRLQLGGGGGGGGGGGGGRRALQWDARGCEPAGHAPRAGSLLAAMKRQNVRTLSLIVCTFTYLLVGAAVFDALESDHEMREEEKLKAEEIRIKGKYNISSEDYRQLELVILQSEPHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTDAGKAFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLLKRIKRCCGLRNTEVSMENMVTVGFFSCMGTLCIGAAAFSQCEEWSFFHAYYYCFITLTTIGFGDYVALQTKGALQKKPLYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNSEDERRDAEERASLAGNRNSMVIHIPEEARQGRPRYKADVADLQSVCSCTCYRPQEYGGRSAAHQNSFSAKLAPQYFHSISYKIEEISPSTLKNSLFPSPISSISPGLHSFTDNHRLMRRRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5N2</accession>
    <name>A0A4W2E5N2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Serine/threonine-protein kinase Chk2</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">CHK2 checkpoint homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Checkpoint kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">CHEK2</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34 35" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24 36">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 36">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="26">Homodimer. Homodimerization is part of the activation process but the dimer may dissociate following activation. Interacts with PML. Interacts with TP53. Interacts with RB1; phosphorylates RB1. Interacts with BRCA1. Interacts (phosphorylated at Thr-68) with MDC1; requires ATM-mediated phosphorylation of CHEK2. Interacts with TP53BP1; modulates CHEK2 phosphorylation at Thr-68 in response to ionizing radiation. Interacts with CDC25A; phosphorylates CDC25A and mediates its degradation in response to ionizing radiation. Interacts with CUL1; mediates CHEK2 ubiquitination and regulation. Interacts with CDKN2AIP. Interacts (via protein kinase domain) with CCAR2 (via N-terminus). Interacts with SIRT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027422222.1">
        <property type="nucleotide sequence ID" value="XM_027566421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5N2"/>
    <dbReference type="SMR" id="A0A4W2E5N2"/>
    <dbReference type="STRING" id="30522.A0A4W2E5N2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001167.1">
        <property type="protein sequence ID" value="ENSBIXP00000031416.1"/>
        <property type="gene ID" value="ENSBIXG00000013682.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015643.1">
        <property type="protein sequence ID" value="ENSBIXP00005000700.1"/>
        <property type="gene ID" value="ENSBIXG00005029873.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907354"/>
    <dbReference type="CTD" id="11200"/>
    <dbReference type="GeneTree" id="ENSGT00800000124190"/>
    <dbReference type="OMA" id="MLCAVQY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22666">
        <property type="entry name" value="FHA_CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14084">
        <property type="entry name" value="STKc_Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000354">
        <property type="entry name" value="Serine/threonine-protein kinase Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000011">
        <property type="entry name" value="Serine/threonine-protein kinase Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000255">
        <property type="entry name" value="serine/threonine-protein kinase Chk2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="22" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="20" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FHA" evidence="31">
        <location>
            <begin position="104"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="211"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="14"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00060832"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00063397"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070912"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00080352"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="534" mass="60251" checksum="557C24C0E7E411C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6W0</accession>
    <name>A0A4W2E6W0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="117"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="405" mass="46707" checksum="3B884ED212FA9F75" modified="2019-09-18" version="1">MGKTRDLFKKIRDTKRTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKRDLHDPDNHDGVITHLEPDILECEVKWALESITMNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALFSHTSKVMLKILQARLQQYVNRELPDVQAGFRKDRGTRDQIANIHWIMEKAREFQKNIYFCFIDYAKAFDCVDHNQLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTEWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFWAPKSLRMVTAAMKLKDAYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E879</accession>
    <name>A0A4W2E879_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">PICALM interacting mitotic regulator</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">PIMREG</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E879"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017072.1">
        <property type="protein sequence ID" value="ENSBIXP00000032259.1"/>
        <property type="gene ID" value="ENSBIXG00000014790.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35819">
        <property type="entry name" value="PICALM INTERACTING MITOTIC REGULATOR PIMREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35819:SF1">
        <property type="entry name" value="PROTEIN PIMREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07326">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="239" mass="26246" checksum="933BE78FBBF5E418" modified="2019-09-18" version="1">MASRWPAVGASLRRRSLQNQERLEESEALQPVASHPDTSSGALGSLCRQFQRRLPLRAVSLNLGVGPSWKRLETPEPGQQGLQAAAHSAKSTLGAMSQRIQESCQSGTKWLVETQVKARRRKRGAQKGSSPPAHSLSQRSTRLSVAAPAHSTLGPWEKEYHRLSVQMSSRAHPWRRSRREAAFRSPYSSAEPLCSPSESDSDLEPVGAGIRHLQKVSQELEEAIVAEDSGDMTVSLIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB35</accession>
    <name>A0A4W2EB35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSBIXT00000008468.1">
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="418" mass="47723" checksum="2AA48E3A60512A12" modified="2019-09-18" version="1">MGSIKDRNGMDLTEAGDTKKRWQEYTEELYKKDIHDPDNHNGVITDLEPDMLECEVKWTLESITMNKASGGDGIPVELFQILKDDAVRVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALISHASKVMLKILQARLQQYVNRELPHVQAGFRKGRGTRDQIANIQWIMEKARSFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMEISDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVHQGCILSPCLFNLYAEYIIRNAGLEDAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVKTVPDFIFLGSKITADGDCSHEIKRCLLLGRKVMTNLDSIFKSRDSTLPTKVRLVKAMVFPVIMLWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDC8</accession>
    <name>A0A4W2EDC8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ninein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NIN</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
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</entry>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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    <name>A0A4W2EI82_BOBOX</name>
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    <reference evidence="19 20" key="1">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and with relatively high calcium permeability. Furthermore, CTP functions as a weak affinity agonist for P2RX1.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Functional P2XRs are organized as homomeric and heteromeric trimers. Forms heterodimer with P2RX2. Forms heterodimer with P2RX4. Forms heterodimer with P2RX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 18">Belongs to the P2X receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EI82"/>
    <dbReference type="SMR" id="A0A4W2EI82"/>
    <dbReference type="STRING" id="30522.A0A4W2EI82"/>
    <dbReference type="GlyCosmos" id="A0A4W2EI82">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000017357.1">
        <property type="protein sequence ID" value="ENSBIXP00000032389.1"/>
        <property type="gene ID" value="ENSBIXG00000014948.1"/>
    </dbReference>
    <dbReference type="OMA" id="DCDLDWS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003044">
        <property type="entry name" value="P2X1_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053792">
        <property type="entry name" value="P2X_RECEPTOR_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF9">
        <property type="entry name" value="P2X PURINOCEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005713">
        <property type="entry name" value="P2X_purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01308">
        <property type="entry name" value="P2X1RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01212">
        <property type="entry name" value="P2X_RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="290"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="17">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="117"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="399" mass="44820" checksum="B377FAD9C13A002A" modified="2019-09-18" version="1">MAQRIQDELASFFFEYDTPRMVLVRNKKVGIVFRLIQLVVLVYVVGWVFVYEKGYQTSGSLISSVSVKLKGLAVTQLPSLGPQVWDVADYVFPVQGDSSFVVMTNFIATPRQAQGHCAENPEGGTCTNNSGCTPGKAERKAQGIRTGKCVAFNDTVQTCEIFGWCPVEVDDNIPCPALLREAENFTLFIKNSISFPRFKVTRRNLVEEVDARYMKTCLFHKTLHPLCPVFKLGYVVQESGQNFSTLAEKGGVVGITIDWHCDLDWHVRHCKPIYEFHGLYEEKKLSQGFNFRFARHFVENGTNYRHLFKVFGIRFDILVDGKAGKFDIIPTMTTIGSGIGIFGVATVLCDLLLLHILPKRHYYKQKKFKYAEDMGPGAGQHDPAATGSTLVLQENMKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIB4</accession>
    <name>A0A4W2EIB4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000037676.1"/>
        <property type="gene ID" value="ENSBIXG00000004041.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="42811" checksum="572152B4C846F66E" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKAFDCVDHKKLWKILKEMGIPDHLIRLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLLNLYAEYIMRNTGLEEAQAGIKIAGRNINHLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASSPNTSWEIDGETVETVSDFIFWGSKITADGDCSHETKRCLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVNKPERQRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRADSLEKTLMLGGIGGRRRRGRQRMRWLDGITDLMDVSLGELRELVMDREAWRAAIHGVAKSLTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJI0</accession>
    <name>A0A4W2EJI0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">APC-binding protein EB1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">End-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MAPRE1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAPRE family.</text>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <sequence length="692" mass="78055" checksum="7352EC92681A4DEC" modified="2019-09-18" version="1"> </sequence>
</entry>
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    <accession>A0A4W2ESV5</accession>
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    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-EC"/>
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    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="119"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="393" mass="45044" checksum="B06481D0693E7107" modified="2019-09-18" version="1">MFKLVSGKAEEPEIKLPTSAGSWKKQEFQKNIYFCFIDYTKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYTGQEATVRTGHGSTDWFQIGKGVRQGCILSPCLFNLHAEYIMRKAGLEEAQAGIKIARRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETAETVSDFIFLGSKITADGHCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESQTVKKAEHQRIDAFELWCWRRLLRVPWTARRSNQSLLKEISPECSLEGMMLKLKLQYFGHLMQRADSLEKTLMLGGNGGRRRRGQQRMRWLDGITDSMDLSLSELRELVMDRETWCAAIHGISKSQTRLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EX08</accession>
    <name>A0A4W2EX08_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SH2 domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SH2D5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in synaptic plasticity regulation through the control of Rac-GTP levels.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with BCR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EX08"/>
    <dbReference type="SMR" id="A0A4W2EX08"/>
    <dbReference type="STRING" id="30522.A0A4W2EX08"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038513.1">
        <property type="protein sequence ID" value="ENSBIXP00000041538.1"/>
        <property type="gene ID" value="ENSBIXG00000025661.1"/>
    </dbReference>
    <dbReference type="OMA" id="QDCGPEG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13157">
        <property type="entry name" value="PTB_tensin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000290">
        <property type="entry name" value="SH2 domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000072">
        <property type="entry name" value="SH2 domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832:SF3">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832">
        <property type="entry name" value="SHC (SRC HOMOLOGY DOMAIN C-TERMINAL) ADAPTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PID" evidence="9">
        <location>
            <begin position="28"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="301"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="397"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00071088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="428" mass="46888" checksum="EF8C706E5AE975A5" modified="2019-09-18" version="1">MQKAGAGGRRASDCGPAPQRPRCITKFAQYVGSFPVDDLDPQESVWLVQQQLWALKDCPRRRAVILKFSLQGLKIYSGEGEVLLMAHALRRILYATWCPADCQFAFMARNPRSPASKLFCHLFVGSQPGEVQILHLLLCRSFQLAYLLQHPEERARPEPCPEPVGDAPLKPVPGPGGPPGLVREPFGRDQLSQNVNALVSFRRLPAEGSGGSGKELLESASRGGTRHLRLGNPYCSPTLVRKKAIRSKVLRSGAYRACTYETQLQLSAREALPAAWEAWPRGPGGPSCLVESEGSLTENIWAFAGISRTSALALLRRDVLGAFLLWPEPGANGQWCLSVRTQCGVVPHQVFRNHVGRYCVEHLPAEFPSLEALVEHHAGTERSLFCSLDMGRLNPGYEEQDCGPSGQPPRTLRPLGHAKSEAELQGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYQ5</accession>
    <name>A0A4W2EYQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Interleukin-10 receptor subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cytokine receptor class-II member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cytokine receptor family 2 member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Interleukin-10 receptor subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">IL10RB</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer with IFNLR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II cytokine receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EYQ5"/>
    <dbReference type="SMR" id="A0A4W2EYQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039853.1">
        <property type="protein sequence ID" value="ENSBIXP00000042195.1"/>
        <property type="gene ID" value="ENSBIXG00000026309.1"/>
    </dbReference>
    <dbReference type="OMA" id="QWDSPAF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004920">
        <property type="term" value="F:interleukin-10 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001312">
        <property type="entry name" value="Interleukin 10 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001363">
        <property type="entry name" value="Interleukin 10 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF50">
        <property type="entry name" value="INTERLEUKIN-10 RECEPTOR SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021311069" description="Interleukin-10 receptor subunit beta" evidence="19">
        <location>
            <begin position="20"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="112"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="317" mass="36132" checksum="644770A050366B3B" modified="2019-09-18" version="1">MARSLLSWLGGCLLMSALGMVPPPENVRMNSVNFKNILRWESPAFPKGNLTFTAQYQSYRKFQDTCTSILLTECDFSSLSKYGDHTLRVRAEFADESSEWINITFCPVDDTTIGPPRMQVEALANSLHVRFFAPRIENEPEPWTMRNIYNSWTYHVRYWKNGSDEKFSISGQYDFEFLRNLESQTTYCVQVRGFLSDRNKAGEWSEPVCEQTTIDETTPSWMVASVLAASVCAALLLLLGCFFLLRCVYRKARHAFPPRNSLPQHLKEFMSHPHHSTLLLFSFPLSDENEVFDKLSVITEVSESCKLNPGAGCGLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZ72</accession>
    <name>A0A4W2EZ72_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Double-stranded RNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="21">3.5.4.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ADAR</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>adenosine in double-stranded RNA + H2O + H(+) = inosine in double-stranded RNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:10120"/>
            <dbReference type="Rhea" id="RHEA-COMP:13885"/>
            <dbReference type="Rhea" id="RHEA-COMP:13886"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.37"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.5.4.37" evidence="21"/>
    <dbReference type="RefSeq" id="XP_027387774.1">
        <property type="nucleotide sequence ID" value="XM_027531973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZ72"/>
    <dbReference type="SMR" id="A0A4W2EZ72"/>
    <dbReference type="STRING" id="30522.A0A4W2EZ72"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039626.1">
        <property type="protein sequence ID" value="ENSBIXP00000042067.1"/>
        <property type="gene ID" value="ENSBIXG00000001625.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009360.1">
        <property type="protein sequence ID" value="ENSBIXP00005029953.1"/>
        <property type="gene ID" value="ENSBIXG00005010010.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886003"/>
    <dbReference type="CTD" id="103"/>
    <dbReference type="GeneTree" id="ENSGT00940000157243"/>
    <dbReference type="OMA" id="ERMQMKR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19913">
        <property type="entry name" value="DSRM_DRADA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19915">
        <property type="entry name" value="DSRM_DRADA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000412">
        <property type="entry name" value="Double-stranded RNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000313">
        <property type="entry name" value="double-stranded RNA-specific adenosine deaminase isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000005">
        <property type="entry name" value="Putative double-stranded RNA-specific adenosine deaminase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044456">
        <property type="entry name" value="ADAR1_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044457">
        <property type="entry name" value="ADAR1_DSRM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042371">
        <property type="entry name" value="Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF107">
        <property type="entry name" value="DOUBLE-STRANDED RNA-SPECIFIC ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02295">
        <property type="entry name" value="z-alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00550">
        <property type="entry name" value="Zalpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50139">
        <property type="entry name" value="Z_BINDING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="15 23" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="18" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Z-binding" evidence="26">
        <location>
            <begin position="130"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Z-binding" evidence="26">
        <location>
            <begin position="241"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="25">
        <location>
            <begin position="452"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="25">
        <location>
            <begin position="563"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="25">
        <location>
            <begin position="675"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="27">
        <location>
            <begin position="835"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="334"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="523"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="620"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066545"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069511"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1175" mass="130452" checksum="D94D785009FB5512" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0L5</accession>
    <name>A0A4W2F0L5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">CD84 molecule</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0L5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041115.1">
        <property type="protein sequence ID" value="ENSBIXP00000042904.1"/>
        <property type="gene ID" value="ENSBIXG00000026919.1"/>
    </dbReference>
    <dbReference type="OMA" id="YTYVMVT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of interleukin-6 production"/>
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        <property type="term" value="P:positive regulation of monocyte chemotactic protein-1 production"/>
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        <property type="term" value="P:positive regulation of tumor necrosis factor production"/>
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        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ig_SLAM-like_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SLAM family member 7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080:SF103">
        <property type="entry name" value="SLAM FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021500042" evidence="13">
        <location>
            <begin position="32"/>
            <end position="351"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
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        <location>
            <begin position="147"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042904.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="351" mass="39373" checksum="BEA951C4BF6DFC64" modified="2019-09-18" version="1">MPCCVQKFIVKTMAHHHLWILLFCLQTCLEAAGSDTDIFIVNGILGESATFPLNIQPSQKVTIISWHSKTSVAFVKPGNFGEEPIVTITHQNYHERISVSGQNYNLELRNLRIEDSGIYKADINVETSKVTTTRSYNLQVYRRLEKPKITQSFVTSVNSTCNVTLMCSVDKEEKNVTYSWSPRGEEGNVLQIFQTPDNQEETYTCTAWNPVSNNSDSISAQQLCADITMTPHTRHTGLMSGLAVLSVLIVILPSVVLFLLCKKGEGSFLKNFSKNPDASSKKTIYTYITVSRNTQPAESRIYDEIPQSKVLPTKEEPVNTIYSIMQYSDKMEKTSTQNSKPLGTSSYEIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F143</accession>
    <name>A0A4W2F143_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Synapsin III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SYN3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synapsin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F143"/>
    <dbReference type="STRING" id="30522.A0A4W2F143"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051943.1">
        <property type="protein sequence ID" value="ENSBIXP00000043096.1"/>
        <property type="gene ID" value="ENSBIXG00000006841.1"/>
    </dbReference>
    <dbReference type="OMA" id="IGSAYKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Synapsin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000042">
        <property type="entry name" value="Synapsin III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000008">
        <property type="entry name" value="Synapsin III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001359">
        <property type="entry name" value="Synapsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020898">
        <property type="entry name" value="Synapsin_ATP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019735">
        <property type="entry name" value="Synapsin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019736">
        <property type="entry name" value="Synapsin_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020897">
        <property type="entry name" value="Synapsin_pre-ATP-grasp_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841:SF27">
        <property type="entry name" value="SYNAPSIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02078">
        <property type="entry name" value="Synapsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02750">
        <property type="entry name" value="Synapsin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10581">
        <property type="entry name" value="Synapsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01368">
        <property type="entry name" value="SYNAPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNAPSIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00416">
        <property type="entry name" value="SYNAPSIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Synapsin pre-ATP-grasp" evidence="7">
        <location>
            <begin position="89"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Synapsin ATP-binding" evidence="8">
        <location>
            <begin position="193"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="400"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="536"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="579" mass="63176" checksum="94DFD953D0096B3A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3H9</accession>
    <name>A0A4W2F3H9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PTPN12</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3H9"/>
    <dbReference type="SMR" id="A0A4W2F3H9"/>
    <dbReference type="STRING" id="30522.A0A4W2F3H9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052367.1">
        <property type="protein sequence ID" value="ENSBIXP00000043608.1"/>
        <property type="gene ID" value="ENSBIXG00000027767.1"/>
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    <dbReference type="OMA" id="PCIIDKI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14604">
        <property type="entry name" value="PTPc-N12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
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        <property type="entry name" value="PTN12"/>
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        <property type="entry name" value="PTN12/18/22"/>
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    <dbReference type="InterPro" id="IPR047251">
        <property type="entry name" value="PTN12_cat"/>
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        <property type="entry name" value="PTP_cat"/>
    </dbReference>
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        <property type="entry name" value="Tyr_Pase_AS"/>
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    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
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    <dbReference type="PANTHER" id="PTHR45983">
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        <property type="entry name" value="Y_phosphatase"/>
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        <property type="entry name" value="Tyr-Ptase_nr12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="28"/>
            <end position="293"/>
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    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
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            <begin position="207"/>
            <end position="284"/>
        </location>
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        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="504"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="588"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="661"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="702"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="626"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="773"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="782" mass="88034" checksum="279A8D9E353D902F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3J9</accession>
    <name>A0A4W2F3J9_BOBOX</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3J9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042443.1">
        <property type="protein sequence ID" value="ENSBIXP00000043597.1"/>
        <property type="gene ID" value="ENSBIXG00000027745.1"/>
    </dbReference>
    <dbReference type="OMA" id="NDYWHSC"/>
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        <property type="component" value="Chromosome 3"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="52" mass="6396" checksum="027295E221909770" modified="2019-09-18" version="1">MYFWGEETLEPKEENIMEAKHICHCSHRNCFNSWHCYPCHDHWHSCIPIDCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3K9</accession>
    <name>A0A4W2F3K9_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113897579</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="Ensembl" id="ENSBIXT00000042460.1">
        <property type="protein sequence ID" value="ENSBIXP00000043607.1"/>
        <property type="gene ID" value="ENSBIXG00000027757.1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="304" mass="34272" checksum="0954768EFD395CCC" modified="2019-09-18" version="1">MAEENMTLVIEFVLTGLTDLPRLQVPLFLVFLVIYLTTMAGNLGLIFLIWKDPHLHTPMYSFLGSLAFVDACSSSSVTPKMLVNTLDKSQMMSLFECMAQYYFFGSSATTECFLLVVMAYDRYAAICSPLLYPVVMSNRLCTCLISASYAIGFLHPIIHVGLLSRLTFCRSNIIHHFYCEILPLFAISCTDPSINALVVFIFAAFIQAFTFTGIIVSCICVLFAILKKKSEKGRSKAFSTCSAHLLSVSLFYCTLFFMYVRPGSGQDQYQDKMYSLFYTIIIPLLNPFIYSLRNKEVLGLNKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FAZ4</accession>
    <name>A0A4W2FAZ4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FAZ4"/>
    <dbReference type="STRING" id="30522.A0A4W2FAZ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053502.1">
        <property type="protein sequence ID" value="ENSBIXP00000026426.1"/>
        <property type="gene ID" value="ENSBIXG00000030056.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013080.1">
        <property type="protein sequence ID" value="ENSBIXP00005002023.1"/>
        <property type="gene ID" value="ENSBIXG00005006188.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111659"/>
    <dbReference type="OMA" id="FSPMGPT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068">
        <property type="entry name" value="BONE MARROW PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068:SF20">
        <property type="entry name" value="PCOTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="162" mass="16544" checksum="0AE53BD5CC7DA861" modified="2019-09-18" version="1">MWILSNLMGVSDGGNLLSTVSPTVKALLGKAGISPIFPFSPRSPFQPLGPRTPGSPWGPRGPASPLGPGFPMGPVGPSSPVGPEGPVLPSGPSGPTGPTSPFSPFCPWGPVSPVRPFSPGGPTSPFGPCFPGGPLEPWSPLMPFASTCPSAPEKEEKEQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FCL5</accession>
    <name>A0A4W2FCL5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transcription factor EB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TFEB</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MiT/TFE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FCL5"/>
    <dbReference type="STRING" id="30522.A0A4W2FCL5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003880.1">
        <property type="protein sequence ID" value="ENSBIXP00000030498.1"/>
        <property type="gene ID" value="ENSBIXG00000012495.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010952.1">
        <property type="protein sequence ID" value="ENSBIXP00005003103.1"/>
        <property type="gene ID" value="ENSBIXG00005000479.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159691"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
        <property type="term" value="P:humoral immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18927">
        <property type="entry name" value="bHLHzip_TFEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000003">
        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024098">
        <property type="entry name" value="bHLHzip_TFEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021802">
        <property type="entry name" value="MiT/TFE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR EB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11851">
        <property type="entry name" value="DUF3371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15951">
        <property type="entry name" value="MITF_TFEB_C_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="469"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="344"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="597"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="683"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="603"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="683"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="713" mass="77904" checksum="BA5E9F3CA30A162F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FCV9</accession>
    <name>A0A4W2FCV9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TBC1 domain family member 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TBC1D30</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398242.1">
        <property type="nucleotide sequence ID" value="XM_027542441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FCV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027618.1">
        <property type="protein sequence ID" value="ENSBIXP00000015946.1"/>
        <property type="gene ID" value="ENSBIXG00000020275.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010775.1">
        <property type="protein sequence ID" value="ENSBIXP00005003199.1"/>
        <property type="gene ID" value="ENSBIXG00005000493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892930"/>
    <dbReference type="CTD" id="23329"/>
    <dbReference type="GeneTree" id="ENSGT00940000159226"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000011">
        <property type="entry name" value="TBC1 domain family member 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000009">
        <property type="entry name" value="TBC1 domain family member 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
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        <property type="entry name" value="Tbc1d30_C"/>
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        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 30"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN TRAP , GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4682"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RabGAP-TBC"/>
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        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
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        <property type="entry name" value="TBC_RABGAP"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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            <begin position="227"/>
            <end position="435"/>
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            <begin position="31"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="801"/>
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        <location>
            <begin position="812"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
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            <begin position="885"/>
            <end position="901"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067508"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="901" mass="100073" checksum="03E20D2551DEF4F7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FH13</accession>
    <name>A0A4W2FH13_BOBOX</name>
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        <recommendedName>
            <fullName evidence="20">Guanine nucleotide-binding protein G(i) subunit alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Adenylate cyclase-inhibiting G alpha protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">GNAI2</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00005004824.1"/>
        <property type="gene ID" value="ENSBIXG00005010693.1"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:ciliary basal body"/>
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        <property type="term" value="C:heterotrimeric G-protein complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:hippocampal mossy fiber to CA3 synapse"/>
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        <property type="entry name" value="Gprotein_alpha_I"/>
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    </dbReference>
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        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00503">
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    </dbReference>
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        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
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        <property type="entry name" value="GPROTEINAI"/>
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        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 22" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="10 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
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        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="124"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="128"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <location>
            <begin position="232"/>
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        <ligand>
            <name>GTP</name>
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        <location>
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            <end position="263"/>
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        <ligand>
            <name>GTP</name>
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            <position position="263"/>
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            <name>GTP</name>
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        <ligand>
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            <position position="408"/>
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            <name>GTP</name>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00039891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="436" mass="48865" checksum="656CD92C5B9DF3E5" modified="2019-09-18" version="1">MAPPPALPRPSPRLDSLRPRPRPALPSVRGGLEGTARRRSELPTRELPVEGRRRERSGVGRGRAGPGPCGGRAAAGPADGGMGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIIHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FIK8</accession>
    <name>A0A4W2FIK8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transducer of ERBB2, 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TOB1</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027373448.1">
        <property type="nucleotide sequence ID" value="XM_027517647.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027373449.1">
        <property type="nucleotide sequence ID" value="XM_027517648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FIK8"/>
    <dbReference type="SMR" id="A0A4W2FIK8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003812.1">
        <property type="protein sequence ID" value="ENSBIXP00000006415.1"/>
        <property type="gene ID" value="ENSBIXG00000012527.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006679.1">
        <property type="protein sequence ID" value="ENSBIXP00005005270.1"/>
        <property type="gene ID" value="ENSBIXG00005011003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877495"/>
    <dbReference type="CTD" id="10140"/>
    <dbReference type="GeneTree" id="ENSGT00940000154208"/>
    <dbReference type="OMA" id="NNGMFPG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060212">
        <property type="term" value="P:negative regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045668">
        <property type="term" value="P:negative regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060390">
        <property type="term" value="P:regulation of SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.90:FF:000001">
        <property type="entry name" value="TOB1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015676">
        <property type="entry name" value="Tob1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17537:SF6">
        <property type="entry name" value="PROTEIN TOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17537">
        <property type="entry name" value="TRANSDUCER OF ERBB2 TOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00960">
        <property type="entry name" value="BTG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01203">
        <property type="entry name" value="BTG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="4">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Anti-proliferative protein" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="341" mass="37791" checksum="CE4B3E45188FD3FC" modified="2019-09-18" version="1">MQLEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPEKPYKGSGFRCIHVGEKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQIGEKGPVKVLYVDDNNENGCELDKEIKNSFNPEAQVFMPVSDPASSVSSSPSPPFGHSAAVSPTFMPRSTQPLTFTTATFAATKFGSTKMKNSGRSNKVARTSPINLGLNVNDLLKQKAISSSMHSLYGLGLGNQQQPQQQQQPSQPPPPPQQQQQQKTSALSPNAKEFIFPNMQGQGSTNGMFPGDSPLNLSPLQYSNAFDVFAAYGGLNEKSFVDGLNFSLNNMQYSNQQFQPVMAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FKY5</accession>
    <name>A0A4W2FKY5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DENN domain containing 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DENND1C</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027403239.1">
        <property type="nucleotide sequence ID" value="XM_027547438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FKY5"/>
    <dbReference type="SMR" id="A0A4W2FKY5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032416.1">
        <property type="protein sequence ID" value="ENSBIXP00000038839.1"/>
        <property type="gene ID" value="ENSBIXG00000022732.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005664.1">
        <property type="protein sequence ID" value="ENSBIXP00005005795.1"/>
        <property type="gene ID" value="ENSBIXG00005011411.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895797"/>
    <dbReference type="CTD" id="79958"/>
    <dbReference type="GeneTree" id="ENSGT00940000161573"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000003">
        <property type="entry name" value="DENN domain containing 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000001">
        <property type="entry name" value="Putative DENN domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040032">
        <property type="entry name" value="DENND1A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF25">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="13"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="473"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="606"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="665"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="727"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="798" mass="86822" checksum="4A3FAB0141D0996D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FR04</accession>
    <name>A0A4W2FR04_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein Abitram</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Actin-binding transcription modulator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein Simiate</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABITRAM</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with F-actin. Interacts with G-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABITRAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FR04"/>
    <dbReference type="SMR" id="A0A4W2FR04"/>
    <dbReference type="STRING" id="30522.A0A4W2FR04"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033759.1">
        <property type="protein sequence ID" value="ENSBIXP00000019893.1"/>
        <property type="gene ID" value="ENSBIXG00000023355.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001371.1">
        <property type="protein sequence ID" value="ENSBIXP00005007919.1"/>
        <property type="gene ID" value="ENSBIXG00005006334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00450000040303"/>
    <dbReference type="OMA" id="GKACEDH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051489">
        <property type="term" value="P:regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000048">
        <property type="entry name" value="Protein Abitram"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039169">
        <property type="entry name" value="Abitram"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651:SF0">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="187" mass="20863" checksum="22CBFD0772D9FF50" modified="2019-09-18" version="1">MTTEPKDPVADLAPEPAVPSLVDRYFTRWYKADVKGKPCEDHCILQHSNRICVITLAGSHPVLQSGKTIKSISYQISTNCSRLQNKVSGKFKRGAQFLTELAPLCKIYCSDGEEYTISSCVRGRLMEVNENILHKPSILQEKPSTEGYIAVVLPKFEESKSITEGLLTQKEYEEVVVKRLRATTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FSE0</accession>
    <name>A0A4W2FSE0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Cadherin EGF LAG seven-pass G-type receptor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Multiple epidermal growth factor-like domains protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">CELSR2</name>
    </gene>
    <organism evidence="35 37">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36 37" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027392104.1">
        <property type="nucleotide sequence ID" value="XM_027536303.1"/>
    </dbReference>
    <dbReference type="STRING" id="30522.A0A4W2FSE0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033793.1">
        <property type="protein sequence ID" value="ENSBIXP00000019949.1"/>
        <property type="gene ID" value="ENSBIXG00000004630.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015894.1">
        <property type="protein sequence ID" value="ENSBIXP00005008410.1"/>
        <property type="gene ID" value="ENSBIXG00005013574.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889522"/>
    <dbReference type="CTD" id="1952"/>
    <dbReference type="GeneTree" id="ENSGT00940000157493"/>
    <dbReference type="OMA" id="FTLYIVE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033326">
        <property type="term" value="P:cerebrospinal fluid secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097475">
        <property type="term" value="P:motor neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021999">
        <property type="term" value="P:neural plate anterior/posterior regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021591">
        <property type="term" value="P:ventricular system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000112">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000005">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000156">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000285">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000020">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000062">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000005">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000089">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000010">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000023">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000029">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000038">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000040">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
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            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="2920" mass="316841" checksum="0C77D4E1730668AD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FY83</accession>
    <name>A0A4W2FY83_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OCM</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FY83"/>
    <dbReference type="STRING" id="30522.A0A4W2FY83"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016615.1">
        <property type="protein sequence ID" value="ENSBIXP00000032079.1"/>
        <property type="gene ID" value="ENSBIXG00000014550.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019633.1">
        <property type="protein sequence ID" value="ENSBIXP00005010978.1"/>
        <property type="gene ID" value="ENSBIXG00005015523.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161875"/>
    <dbReference type="OMA" id="CTCISIL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16255">
        <property type="entry name" value="EFh_parvalbumin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF4">
        <property type="entry name" value="ONCOMODULIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
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            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
            <position position="91"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
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        </ligand>
    </feature>
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        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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    </feature>
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        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="109" mass="12099" checksum="4BF3D72BE9B94A72" modified="2019-09-18" version="1">MSITDVLSADDIAAALQECQDPDTFEPQKFFQTSGLAKMSASQVKDVFRFIDNDQSGYLDEEELKFFLQKFESGARELTESETKSLMAAADNDGDGKIGADEFQEMVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G524</accession>
    <name>A0A4W2G524_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein turtle homolog A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Immunoglobulin superfamily member 9A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">IGSF9</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Functions in dendrite outgrowth and synapse maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the immunoglobulin superfamily. Turtle family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384674.1">
        <property type="nucleotide sequence ID" value="XM_027528873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G524"/>
    <dbReference type="SMR" id="A0A4W2G524"/>
    <dbReference type="STRING" id="30522.A0A4W2G524"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040996.1">
        <property type="protein sequence ID" value="ENSBIXP00000042833.1"/>
        <property type="gene ID" value="ENSBIXG00000026824.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023006.1">
        <property type="protein sequence ID" value="ENSBIXP00005013245.1"/>
        <property type="gene ID" value="ENSBIXG00005017383.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884339"/>
    <dbReference type="CTD" id="57549"/>
    <dbReference type="GeneTree" id="ENSGT00940000160444"/>
    <dbReference type="OMA" id="FVMGPNV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060077">
        <property type="term" value="C:inhibitory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050807">
        <property type="term" value="P:regulation of synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000272">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000323">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000389">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001243">
        <property type="entry name" value="Protein turtle homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000226">
        <property type="entry name" value="protein turtle homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000245">
        <property type="entry name" value="protein turtle homolog B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000321">
        <property type="entry name" value="protein turtle homolog B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF244">
        <property type="entry name" value="PROTEIN TURTLE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
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    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="1179"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="735"/>
            <end position="760"/>
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    </feature>
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            <begin position="24"/>
            <end position="110"/>
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    <feature type="domain" description="Ig-like" evidence="24">
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            <begin position="136"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="226"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="322"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="418"/>
            <end position="502"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="507"/>
            <end position="611"/>
        </location>
    </feature>
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        <location>
            <begin position="623"/>
            <end position="718"/>
        </location>
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            <begin position="604"/>
            <end position="626"/>
        </location>
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        <location>
            <begin position="768"/>
            <end position="915"/>
        </location>
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            <begin position="1015"/>
            <end position="1079"/>
        </location>
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            <begin position="610"/>
            <end position="626"/>
        </location>
    </feature>
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            <begin position="782"/>
            <end position="800"/>
        </location>
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        <location>
            <begin position="824"/>
            <end position="842"/>
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            <end position="915"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00034103"/>
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            <dbReference type="ARBA" id="ARBA00057902"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061549"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075030"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1179" mass="127056" checksum="3030BC49022853F8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G9L2</accession>
    <name>A0A4W2G9L2_BOBOX</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TBX1</name>
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    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Bovinae</taxon>
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    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
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        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <begin position="108"/>
            <end position="291"/>
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        </source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="486" mass="51933" checksum="0F45731A6AC9B53F" modified="2019-09-18" version="1">MHFSTVTRDMEAFTASSLSGLGAAGGFPGAASPGADPYGAREPPPPRYEPCSAAAPGAPGPPHAYPFAPAAGAASSAAEPEGPGSSCVAAAKAPVKKNAKVASVSVQLEMKALWDEFNQLGTEMIVTKAGRRMFPTFQVKLFGMDPMADYMLLMDFVPVDDKRYRYAFHSSSWLVAGKADPATPGRVHYHPDSPAKGAQWMKQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVYVDPRKDSEKYAEENFKTFVFEETRFTAVTAYQNHRITQLKIASNPFAKGFRDCDPEDWPRNHRPGALPIMSAFARSRNPVASPTQPNGAEKDTAEARREFERDAGGPAGLGDPAHPPQLLARVLSPALPGPGVPLPGAPGGRPSPPHPELRLEAPGASEPLHHHPYKYPAAAYDHYLGAKSRPAPYPLPGLRGHGFHAHPHPHAHPHHHPAVSPAAAAAAAAAAAAAASMYSSAGAAPPGSYDYCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GA85</accession>
    <name>A0A4W2GA85_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anterior gradient 2, protein disulphide isomerase family member</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AGR2</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AGR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395799.1">
        <property type="nucleotide sequence ID" value="XM_027539998.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395800.1">
        <property type="nucleotide sequence ID" value="XM_027539999.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395801.1">
        <property type="nucleotide sequence ID" value="XM_027540000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GA85"/>
    <dbReference type="SMR" id="A0A4W2GA85"/>
    <dbReference type="STRING" id="30522.A0A4W2GA85"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038789.1">
        <property type="protein sequence ID" value="ENSBIXP00000023278.1"/>
        <property type="gene ID" value="ENSBIXG00000025770.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047332.1">
        <property type="protein sequence ID" value="ENSBIXP00005014776.1"/>
        <property type="gene ID" value="ENSBIXG00005018693.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891657"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063273"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0002162">
        <property type="term" value="F:dystroglycan binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:epidermal growth factor receptor binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048546">
        <property type="term" value="P:digestive tract morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:lung goblet cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:mucus secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of developmental growth"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903896">
        <property type="term" value="P:positive regulation of IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903899">
        <property type="term" value="P:positive regulation of PERK-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02960">
        <property type="entry name" value="AGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000036">
        <property type="entry name" value="anterior gradient protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051099">
        <property type="entry name" value="AGR/TXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15337:SF1">
        <property type="entry name" value="ANTERIOR GRADIENT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15337">
        <property type="entry name" value="ANTERIOR GRADIENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611576" evidence="7">
        <location>
            <begin position="21"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="175" mass="19937" checksum="885DB1C03C2E14A3" modified="2019-09-18" version="1">MEKIPVSAFLLLVALSYTLAKDTTVKPGAKKDTKDSQLKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKAFAENKEIQRLAEQFVLLNLVYETTDKHLSPDGQYVPRILFVDPSLTVRADITGRYSNRLYAYEPSDTALLLDNMKKALKLLKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GSY1</accession>
    <name>A0A4W2GSY1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NIPA like domain containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NIPAL4</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401621.1">
        <property type="nucleotide sequence ID" value="XM_027545820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GSY1"/>
    <dbReference type="STRING" id="30522.A0A4W2GSY1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052159.1">
        <property type="protein sequence ID" value="ENSBIXP00000025381.1"/>
        <property type="gene ID" value="ENSBIXG00000005764.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035175.1">
        <property type="protein sequence ID" value="ENSBIXP00005021371.1"/>
        <property type="gene ID" value="ENSBIXG00005024348.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895096"/>
    <dbReference type="CTD" id="348938"/>
    <dbReference type="GeneTree" id="ENSGT00940000159087"/>
    <dbReference type="OMA" id="MGAGEVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF7">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="404" mass="44002" checksum="88739C7034A605B9" modified="2019-09-18" version="1">MELPASNSSCENGSLISLYCSSQKVLCQIISDLQPEVPSNVTSNSWQERFRKNYSFYVGLGLAILSSFLVGSSIILKKKGLQRLVASGATRAVDGGYGYLKDSMWWAGFLTMAAGEVANFGAYAFAPATVVTPLGALSILISAIFSSYFLGESLNLLGKLGCVICVAGSTVMVIHAPEEEKISTIMEMAAKMKDTGYIVFAVFLLVSCLILIFVVAPRYGQRNILIYITICSVIGAFSVSSSKGLGITIRNFFQGLPVVRHPLPYILSLMLALSISTQVNFLNRALDIFNTSLVFPIYYVFFTTTVMTSSIILFKEWYRMSAVDIVGTLSGFVTIILGVFMLHAFKDLDISRSSLPHMHKNPPATPAPEPSVIRLGDKNVLVDNMELSSTPSPEEKPKVFIAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GV96</accession>
    <name>A0A4W2GV96_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fucose mutarotase</fullName>
            <ecNumber evidence="3">5.1.3.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FUOM</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose (6-deoxy-L-galactose) exists as alpha-L-fucose (29.5%) and beta-L-fucose (70.5%), the beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-L-fucose = beta-L-fucose</text>
            <dbReference type="Rhea" id="RHEA:25580"/>
            <dbReference type="ChEBI" id="CHEBI:42548"/>
            <dbReference type="ChEBI" id="CHEBI:42589"/>
            <dbReference type="EC" id="5.1.3.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; L-fucose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Mainly homodimer, but also exists as homotetramer, homooctamer, and homodecamer. The homodimeric form seems catalytically inactive.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RbsD / FucU family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.29" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027385374.1">
        <property type="nucleotide sequence ID" value="XM_027529573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GV96"/>
    <dbReference type="SMR" id="A0A4W2GV96"/>
    <dbReference type="STRING" id="30522.A0A4W2GV96"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004119.1">
        <property type="protein sequence ID" value="ENSBIXP00000030406.1"/>
        <property type="gene ID" value="ENSBIXG00000006972.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036503.1">
        <property type="protein sequence ID" value="ENSBIXP00005022245.1"/>
        <property type="gene ID" value="ENSBIXG00005025168.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884705"/>
    <dbReference type="CTD" id="282969"/>
    <dbReference type="GeneTree" id="ENSGT00390000001197"/>
    <dbReference type="OMA" id="PVWDTYT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036373">
        <property type="term" value="F:L-fucose mutarotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060180">
        <property type="term" value="P:female mating behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045665">
        <property type="term" value="P:negative regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1650.10:FF:000005">
        <property type="entry name" value="Fucose mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1650.10">
        <property type="entry name" value="RbsD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023750">
        <property type="entry name" value="RbsD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050443">
        <property type="entry name" value="RbsD/FucU_mutarotase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007721">
        <property type="entry name" value="RbsD_FucU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31690">
        <property type="entry name" value="FUCOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31690:SF4">
        <property type="entry name" value="FUCOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05025">
        <property type="entry name" value="RbsD_FucU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102546">
        <property type="entry name" value="RbsD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="154" mass="16641" checksum="05A2FE3C5B0F4E95" modified="2019-09-18" version="1">MVVLKGVPALLSPELLFALARMGHGDEIVLADVNFPSSSICRGGPEEIRADGLGIPQLLEAVLQLLPLDTYVQSPAMVMELVPSDRKSGLLTPVWTSYQSILSRAGYEFSLGMVERFAFYERAKKAFAVVATGETALYGNLILKKGVLAPKDLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H4X2</accession>
    <name>A0A4W2H4X2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adenine nucleotide translocase lysine methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ANTKMT</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANT/ATPSC lysine N-methyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H4X2"/>
    <dbReference type="SMR" id="A0A4W2H4X2"/>
    <dbReference type="STRING" id="30522.A0A4W2H4X2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014837.1">
        <property type="protein sequence ID" value="ENSBIXP00000001066.1"/>
        <property type="gene ID" value="ENSBIXG00000028997.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040685.1">
        <property type="protein sequence ID" value="ENSBIXP00005025121.1"/>
        <property type="gene ID" value="ENSBIXG00005027657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014771"/>
    <dbReference type="OMA" id="HEFRIPG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905706">
        <property type="term" value="P:regulation of mitochondrial ATP synthesis coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610:SF5">
        <property type="entry name" value="ADENINE NUCLEOTIDE TRANSLOCASE LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="226" mass="24316" checksum="B1CD501A2E469A58" modified="2019-09-18" version="1">MEQDDPAEALTELRERRPGPLELLQVAAGSGLAVYAVWALLLQPGFRRVPLRLQVPYVGASARQVEHVLSLLRGRPGKTVDLGSGDGRIVLAAHRCGLRPAVGYELNPWLVGLARLRAWRAGCAGSVRYHREDLWKVSLRDCHNVSVFLAPSVLPLLEDKLQAELPAGARVVSGRFPLPTWQPVAVVGEGLDRVWAYDIHRGGPAGQAVPGPSSASVLGTPNSQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HBU1</accession>
    <name>A0A4W2HBU1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HBU1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045725.1">
        <property type="protein sequence ID" value="ENSBIXP00000012727.1"/>
        <property type="gene ID" value="ENSBIXG00000017778.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055395.1">
        <property type="protein sequence ID" value="ENSBIXP00005027243.1"/>
        <property type="gene ID" value="ENSBIXG00005030871.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159622"/>
    <dbReference type="OMA" id="WNQMVET"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000370">
        <property type="entry name" value="CMRF35-like molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF115">
        <property type="entry name" value="IMMUNOGLOBULIN SUBTYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611851" description="Immunoglobulin domain-containing protein" evidence="6">
        <location>
            <begin position="30"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="7">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="176" mass="19488" checksum="909CD640B91B1D1D" modified="2019-09-18" version="1">PHLGLRKGLWTRLCFTGCLSLSGPRSVTGIVGGSMSVECQYQEAFVDDIKYWCKHPCVSPWKIVETRESEREVRRGRVSIRDRPASLTFTVTLESLREEDEGTYGCGIYVPLSRDPTFQVEVSVIPGGTAVSWRAPGAPASRWWSWGSAAAARGGRWSPGAHVGLWMLWRPLPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HDC4</accession>
    <name>A0A4W2HDC4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small membrane A-kinase anchor protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">C2orf88</name>
        <name evidence="9" type="synonym">C2H2orf88</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small membrane AKAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HDC4"/>
    <dbReference type="SMR" id="A0A4W2HDC4"/>
    <dbReference type="STRING" id="30522.A0A4W2HDC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052690.1">
        <property type="protein sequence ID" value="ENSBIXP00000026119.1"/>
        <property type="gene ID" value="ENSBIXG00000028211.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008586.1">
        <property type="protein sequence ID" value="ENSBIXP00005030202.1"/>
        <property type="gene ID" value="ENSBIXG00005000827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011537"/>
    <dbReference type="OMA" id="QTFPFPN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027969">
        <property type="entry name" value="Small_membr_AKAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36471">
        <property type="entry name" value="SMALL MEMBRANE A-KINASE ANCHOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36471:SF1">
        <property type="entry name" value="SMALL MEMBRANE A-KINASE ANCHOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15127">
        <property type="entry name" value="SmAKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="95" mass="10969" checksum="1AB45BB1678F1C50" modified="2019-09-18" version="1">MGCMKSKQTFPFPTTLESEKRHVSEENFMPEEKFLPRIPSLVNVKEEVKEPPGPQIVVFEFAQHLSQEILSDALQQWAGNNLKYYDIPYIESEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HHV3</accession>
    <name>A0A4W2HHV3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TBC1 domain family member 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TBC1D9</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027423206.1">
        <property type="nucleotide sequence ID" value="XM_027567405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HHV3"/>
    <dbReference type="STRING" id="30522.A0A4W2HHV3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005780.1">
        <property type="protein sequence ID" value="ENSBIXP00000029876.1"/>
        <property type="gene ID" value="ENSBIXG00000011621.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010944.1">
        <property type="protein sequence ID" value="ENSBIXP00005029394.1"/>
        <property type="gene ID" value="ENSBIXG00005009304.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907791"/>
    <dbReference type="CTD" id="23158"/>
    <dbReference type="GeneTree" id="ENSGT00940000157878"/>
    <dbReference type="OMA" id="HMCFYAY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13351">
        <property type="entry name" value="PH-GRAM1_TCB1D9_TCB1D9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13354">
        <property type="entry name" value="PH-GRAM2_TCB1D9_TCB1D9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000013">
        <property type="entry name" value="Putative TBC1 domain family member 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000008">
        <property type="entry name" value="TBC1 domain family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000119">
        <property type="entry name" value="TBC1 domain family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000041">
        <property type="entry name" value="TBC1 domain family member 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000002">
        <property type="entry name" value="TBC1 domain family member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000033">
        <property type="entry name" value="TBC1 domain family member 9B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036014">
        <property type="entry name" value="TCB1D9/TCB1D9B_PH-GRAM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036017">
        <property type="entry name" value="TCB1D9/TCB1D9B_PH-GRAM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF3">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="515"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="886"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="415"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1072"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1136"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1077"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1136"/>
            <end position="1145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1267" mass="143214" checksum="DD7F0A6AD1BEF375" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HN78</accession>
    <name>A0A4W2HN78_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="26">Hyperpolarization activated cyclic nucleotide gated potassium channel 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">HCN3</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386855.1">
        <property type="nucleotide sequence ID" value="XM_027531054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HN78"/>
    <dbReference type="SMR" id="A0A4W2HN78"/>
    <dbReference type="STRING" id="30522.A0A4W2HN78"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039901.1">
        <property type="protein sequence ID" value="ENSBIXP00000042215.1"/>
        <property type="gene ID" value="ENSBIXG00000026329.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004374.1">
        <property type="protein sequence ID" value="ENSBIXP00005031559.1"/>
        <property type="gene ID" value="ENSBIXG00005011927.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885569"/>
    <dbReference type="CTD" id="57657"/>
    <dbReference type="GeneTree" id="ENSGT00940000162023"/>
    <dbReference type="OMA" id="HMANHSW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071320">
        <property type="term" value="P:cellular response to cAMP"/>
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    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.287.70:FF:000031">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, putative"/>
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    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
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        <property type="entry name" value="Putative potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
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        <property type="entry name" value="K/Na_HCN_channel"/>
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    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
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    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF7">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 3"/>
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        <property type="entry name" value="cNMP_binding"/>
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        <property type="entry name" value="Ion_trans"/>
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        <property type="entry name" value="EAGCHANLFMLY"/>
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        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
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    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
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        <location>
            <begin position="430"/>
            <end position="536"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="552"/>
            <end position="572"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="647"/>
            <end position="666"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="691"/>
            <end position="783"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="11"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="14"/>
            <end position="36"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="647"/>
            <end position="661"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031559.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="783" mass="86777" checksum="01547C5FEA9DA677" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I6F5</accession>
    <name>A0A4W2I6F5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="17">Disintegrin and metalloproteinase domain-containing protein 17</fullName>
            <ecNumber evidence="16">3.4.24.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TNF-alpha convertase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">TNF-alpha-converting enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ADAM17</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNFalpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.</text>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
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        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000016975.1"/>
        <property type="gene ID" value="ENSBIXG00000003967.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049229.1">
        <property type="protein sequence ID" value="ENSBIXP00005039389.1"/>
        <property type="gene ID" value="ENSBIXG00005002613.1"/>
    </dbReference>
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    <dbReference type="CTD" id="6868"/>
    <dbReference type="GeneTree" id="ENSGT00940000155443"/>
    <dbReference type="OMA" id="EHDPDIT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045121">
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        <property type="term" value="C:ruffle membrane"/>
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        <property type="term" value="F:interleukin-6 receptor binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005112">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043120">
        <property type="term" value="F:tumor necrosis factor binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cell adhesion mediated by integrin"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cellular response to high density lipoprotein particle stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071679">
        <property type="term" value="P:commissural neuron axon guidance"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:defense response to Gram-positive bacterium"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:ERBB2-EGFR signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:germinal center formation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of neuron projection development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:Notch receptor processing"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
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        <property type="term" value="P:regulation of mast cell apoptotic process"/>
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        <property type="term" value="P:response to lipopolysaccharide"/>
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        <property type="term" value="P:response to xenobiotic stimulus"/>
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        <property type="term" value="P:spleen development"/>
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        <property type="term" value="P:T cell differentiation in thymus"/>
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        <property type="term" value="P:wound healing, spreading of epidermal cells"/>
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        <property type="entry name" value="ADAM17_MPD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZnMc_TACE_like"/>
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        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Disintegrin domain"/>
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        <property type="entry name" value="ADAM10_ADAM17"/>
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        <property type="entry name" value="ADAM_Metalloproteinase"/>
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        <property type="entry name" value="Disintegrin_dom"/>
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        <property type="entry name" value="Disintegrin_dom_sf"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Peptidase_M12B"/>
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        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Reprolysin_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
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    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="10" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
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    <keyword evidence="7 23" id="KW-0732">Signal</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
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    <keyword evidence="9 20" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
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    <feature type="chain" id="PRO_5044612051" description="Disintegrin and metalloproteinase domain-containing protein 17" evidence="23">
        <location>
            <begin position="18"/>
            <end position="825"/>
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        <location>
            <begin position="670"/>
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        <location>
            <begin position="221"/>
            <end position="472"/>
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        <location>
            <begin position="473"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="728"/>
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        <location>
            <begin position="769"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="792"/>
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        <location>
            <begin position="816"/>
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        <location>
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        <location>
            <position position="403"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
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            <name>Zn(2+)</name>
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            <position position="413"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075778"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="825" mass="92998" checksum="47EB1F6E8DA17103" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I8T6</accession>
    <name>A0A4W2I8T6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TIMM8B</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Probable mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer; possibly composed of 3 copies of TIMM8B and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I8T6"/>
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        <property type="protein sequence ID" value="ENSBIXP00000029319.1"/>
        <property type="gene ID" value="ENSBIXG00000010898.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029061.1">
        <property type="protein sequence ID" value="ENSBIXP00005038670.1"/>
        <property type="gene ID" value="ENSBIXG00005020798.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155479"/>
    <dbReference type="OMA" id="NEICWDK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000005">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim8 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="16">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="96" mass="11055" checksum="F7872AB2D502F216" modified="2019-09-18" version="1">MRRLRKVGNWTRKMAELGEADEAELQRLVAAEQQKAQFTAQVHHFMELCWDKCVEKPGNRLDSRTENCLSSCVDRFIDTTLAITSRFAQIVQRGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IEF6</accession>
    <name>A0A4W2IEF6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Target of myb1 membrane trafficking protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TOM1</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TOM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397644.1">
        <property type="nucleotide sequence ID" value="XM_027541843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IEF6"/>
    <dbReference type="SMR" id="A0A4W2IEF6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041296.1">
        <property type="protein sequence ID" value="ENSBIXP00000024896.1"/>
        <property type="gene ID" value="ENSBIXG00000027029.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035442.1">
        <property type="protein sequence ID" value="ENSBIXP00005041461.1"/>
        <property type="gene ID" value="ENSBIXG00005004200.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892676"/>
    <dbReference type="CTD" id="10043"/>
    <dbReference type="GeneTree" id="ENSGT00940000156865"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14236">
        <property type="entry name" value="GAT_TOM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16995">
        <property type="entry name" value="VHS_Tom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000001">
        <property type="entry name" value="TOM1-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000003">
        <property type="entry name" value="TOM1-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014645">
        <property type="entry name" value="TOM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856:SF32">
        <property type="entry name" value="TARGET OF MYB1 MEMBRANE TRAFFICKING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856">
        <property type="entry name" value="VHS DOMAIN CONTAINING PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036948">
        <property type="entry name" value="TOM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="6">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="7">
        <location>
            <begin position="215"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="454"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005041461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="492" mass="54212" checksum="5A7DB8BA95F58945" modified="2019-09-18" version="1">MDFLLGNPFSSPVGQRIEKATDGSLQSEDWALNMEICDIINETEEGPKDAFRAVKKRIVGNKNFHEVMLALTVLETCVKNCGHRFHVLVASQDFVEGVLVRTILPKNNPPTIVHDKVLTLIQSWADAFRSSPDLTGVVAVYEDLRRKGLEFPMTDLDMLSPIHTPQRTVFNSETQSGQNSVGTDTSHRGDSNQQTTPLHTSALLPSDTPITPTPEQIGKLRSELEMVSGNVRVMSEMLTELVPTQTEPADLELLQELNRTCRSMQQRVLELIPRIANEQLTEELLIVNDNLNNVFLRHERFERLRTGQTTKAPSEAEAAPDLIDMGPDPVATGNLSSQLAEMNLGSSSVRAGLQSLEASGRLEDEFDMFALTRGSSLADQRKEVKYEAPQATDGLAGALDARQQSTGAIPVTQACLMEDIEKWLSTDVGNDVEEPKGVTSEEFDKFLEERAKVADRLPNLSSPSTEGPPGPPPGTALRKKTQEKDDDMLFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2II63</accession>
    <name>A0A4W2II63_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Keratin-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">KRTAP11-1</name>
        <name evidence="5" type="synonym">LOC113898381</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with hair keratins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PMG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2II63"/>
    <dbReference type="STRING" id="30522.A0A4W2II63"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040178.1">
        <property type="protein sequence ID" value="ENSBIXP00000024187.1"/>
        <property type="gene ID" value="ENSBIXG00000026429.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053713.1">
        <property type="protein sequence ID" value="ENSBIXP00005044974.1"/>
        <property type="gene ID" value="ENSBIXG00005004999.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111463"/>
    <dbReference type="OMA" id="CQPVGGI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007659">
        <property type="entry name" value="Keratin_matx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007951">
        <property type="entry name" value="KRTAP_PMG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23260">
        <property type="entry name" value="KERATIN ASSOCIATED PROTEIN 3-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23260:SF9">
        <property type="entry name" value="KERATIN-ASSOCIATED PROTEIN 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05287">
        <property type="entry name" value="PMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="159" mass="16886" checksum="BF74D913C0953F1C" modified="2019-09-18" version="1">MSYNCSTRTCSSRRIGGEYTVPVATVSTPDADCLSGIYLPSSFQTGSWLLDHCQETCCEPTLCQSTCYQPAPCVSNPVQVTSRQTTCVSSPYSTTCSRPVTFVSSGCQPLSGISTVCKPVRSISTVCQPVGGVSTICQPACGVSRTYQQSCVSSCRRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BJQ6</accession>
    <name>A0A4W2BJQ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="32">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24 32">Postsynaptic cell membrane</location>
            <topology evidence="24 32">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="28">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2BJQ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053970.1">
        <property type="protein sequence ID" value="ENSBIXP00000000030.1"/>
        <property type="gene ID" value="ENSBIXG00000030351.1"/>
    </dbReference>
    <dbReference type="OMA" id="MPDEFPS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007611">
        <property type="term" value="P:learning or memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045471">
        <property type="term" value="P:response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06378">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13718">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000593">
        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000344">
        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000020">
        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2B, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000007">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000009">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018884">
        <property type="entry name" value="NMDAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10565">
        <property type="entry name" value="NMDAR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 32" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16 31" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22 32" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14 32" id="KW-0406">Ion transport</keyword>
    <keyword evidence="21 32" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19 32" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="17 32" id="KW-0675">Receptor</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 32" id="KW-0732">Signal</keyword>
    <keyword evidence="13 32" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 32" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="32">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027142759" description="Glutamate receptor" evidence="32">
        <location>
            <begin position="25"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="820"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="34">
        <location>
            <begin position="431"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="35">
        <location>
            <begin position="439"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1021"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="1033"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="1052"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="1070"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="689"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="690"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="731"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="30">
        <location>
            <position position="662"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="30">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="31">
        <location>
            <begin position="745"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00060784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1464" mass="164718" checksum="6382F00CDC5071A0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BN88</accession>
    <name>A0A4W2BN88_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cohesin subunit SA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SCC3 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Stromal antigen</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">STAG3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the meiosis-specific cohesin complex, which also contains the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer. Such complex likely contains RAD21, or the meiosis-specific related protein REC8. Interacts with CCDC79/TERB1; recruiting cohesin to telomeres to develop structural rigidity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the cohesin complex which is composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
            <location evidence="11">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SCC3 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000000440.1"/>
        <property type="gene ID" value="ENSBIXG00000029821.1"/>
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        <property type="component" value="Chromosome 25"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:meiotic cohesin complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:establishment of meiotic sister chromatid cohesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Cohesin subunit SA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF8">
        <property type="entry name" value="COHESIN SUBUNIT SA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="79"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
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            <begin position="1052"/>
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        </location>
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        <location>
            <begin position="1108"/>
            <end position="1122"/>
        </location>
    </feature>
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        <location>
            <begin position="1138"/>
            <end position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057292"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064253"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU369063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1182" mass="133234" checksum="FE66BE936BE04EC5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNM4</accession>
    <name>A0A4W2BNM4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113878298</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
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        <location>
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            <end position="218"/>
        </location>
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            <begin position="40"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="310" mass="34495" checksum="509B3AE61C9872A2" modified="2019-09-18" version="1">MRDENQSSVLDFFLLGVSSHQEQEDFFVLFLFIYSITLIGNLLILAIHSDIRLHNPMYFLLANLSFVDIFFSSVTIPKALANHLLGTKAISFGGCLTQMYFMIALANTDSYILAAMAYDRAVAITRPLQYTTIMSPWTCVLLVIGSWVVGNGNALPHTLLTASLSFCGNQEVANFYCDIASLLKLSCSDIHFNVKMMYLGVGVFSMPLLCIIISYVGVFSTVLRVPSTKGVLKAFSTCGSHLTVVSLYYGTVMGMYFRPLTSYSLKDAVITVMYIAVTPMLNPFIYTLRNRVMKDALEKLFSKRTSSHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BP58</accession>
    <name>A0A4W2BP58_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tryptase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.</text>
            <dbReference type="EC" id="3.4.21.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.59" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BP58"/>
    <dbReference type="STRING" id="30522.A0A4W2BP58"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052999.1">
        <property type="protein sequence ID" value="ENSBIXP00000000515.1"/>
        <property type="gene ID" value="ENSBIXG00000029748.1"/>
    </dbReference>
    <dbReference type="OMA" id="PVCMPDP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF119">
        <property type="entry name" value="SERINE PROTEASE 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021405173" description="tryptase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="36"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00050838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="311" mass="34000" checksum="E92EB444EF1B8C87" modified="2019-09-18" version="1">MRQVLAAVLLLLLLRSGTRGGEALNACGRPRMLNRMVGGQNALEGEWPWQVSIQRNGSHFCGGSLITDRWVLTAAHCFSNTSETSLYQVLLGVLQLARPGPHAVYARVKQVESNPQYQGMASSADVALVELEAPVTFTNYILPVCVPDPSVVFESGMKCWVTGWGTPSEQDSLPKPRTLQKLAVPIISTPKCNLLYSKDAESGFQPRTIKDDMLCAGFAEGKRDACKGDSGGPLVCLVGQVWLQAGVISWGEGCARRNRPGVYIRLTSHHDWIHRIIPELQFQRAGPGSAQKRDPRTRQPPGRNSARYPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BPV1</accession>
    <name>A0A4W2BPV1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Microtubule-associated protein RP/EB family member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MAPRE2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BPV1"/>
    <dbReference type="STRING" id="30522.A0A4W2BPV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012679.1">
        <property type="protein sequence ID" value="ENSBIXP00000002046.1"/>
        <property type="gene ID" value="ENSBIXG00000008581.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026515.1">
        <property type="protein sequence ID" value="ENSBIXP00005015585.1"/>
        <property type="gene ID" value="ENSBIXG00005019431.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043329"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032014">
        <property type="term" value="P:positive regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120183">
        <property type="term" value="P:positive regulation of focal adhesion disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051549">
        <property type="term" value="P:positive regulation of keratinocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000002">
        <property type="entry name" value="microtubule-associated protein RP/EB family member 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="56"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="13">
        <location>
            <begin position="235"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="170"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="322" mass="36393" checksum="9B760BE3C8718469" modified="2019-09-18" version="1">MPGPTQTLSPNGENNNDIIQDNGTIIPFRKHTVRGERSYSWGMAVNVYSTSITQETMSRHDIIAWVNDIVSLNYTKVEQLCSGAAYCQFMDMLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKVIPVEKLVKGRFQDNLDFIQWFKKFYDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKSSPASKPGSTPSRPSSAKRASSSGSASRSDKDLETQVIQLNEQVHSLKLALEGVEKERDFYFGKLREIELLCQEHGQENDDLVQRLMDVLYASDEHDNFSCKSGSGQLQKKYFRLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQ41</accession>
    <name>A0A4W2BQ41_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PHD and RING finger domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PHRF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="6">Interacts with POLR2A (via the C-terminal domain).</text>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
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    </dbReference>
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        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="17">
        <location>
            <begin position="63"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="199"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="16">
        <location>
            <begin position="430"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="578" mass="65482" checksum="DE12B7A83D91E9B5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BR28</accession>
    <name>A0A4W2BR28_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BR28"/>
    <dbReference type="STRING" id="30522.A0A4W2BR28"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011856.1">
        <property type="protein sequence ID" value="ENSBIXP00000002472.1"/>
        <property type="gene ID" value="ENSBIXG00000000202.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035708.1">
        <property type="protein sequence ID" value="ENSBIXP00005021715.1"/>
        <property type="gene ID" value="ENSBIXG00005024661.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244869"/>
    <dbReference type="OMA" id="MCAYGPI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="7"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="279" mass="30896" checksum="CCF1086167219A79" modified="2019-09-18" version="1">MRNHTELNEFILLGIPQTEGMKTVLLGIFSLIYPLTLLGNLLILLAVMLVYFSGQNRAISYKGCAVQLFFYHFLGSTEGCFYSVMAYDRFVAICHPLRYKLIMRPGVCVGLVLAAWLVGCLQATILTSFTFQLPYCGPNQVDHFFCDIPVILPLACADSSFAQGVASTSVGFLALMLWLSVCVSYTRIGIAILRIHSAEGRQKAFSTCSAHLTAILCAFGPIIIVYLQPTPNPLLDAMVQLLNNTVSPMLNSLIYSLRNKEVKSSLKRIFHNVVFTVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BT86</accession>
    <name>A0A4W2BT86_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Speckle targeted PIP5K1A-regulated poly(A) polymerase</fullName>
            <ecNumber evidence="6">2.7.7.19</ecNumber>
            <ecNumber evidence="7">2.7.7.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RNA-binding motif protein 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">U6 snRNA-specific terminal uridylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>RNA(n) + UTP = RNA(n)-3'-uridine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14785"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17348"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173116"/>
            <dbReference type="EC" id="2.7.7.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="24">Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="6"/>
    <dbReference type="EC" id="2.7.7.52" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BT86"/>
    <dbReference type="SMR" id="A0A4W2BT86"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011829.1">
        <property type="protein sequence ID" value="ENSBIXP00000002481.1"/>
        <property type="gene ID" value="ENSBIXG00000008949.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050265">
        <property type="term" value="F:RNA uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12279">
        <property type="entry name" value="RRM_TUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000008">
        <property type="entry name" value="speckle targeted PIP5K1A-regulated poly(A) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000305">
        <property type="entry name" value="speckle targeted PIP5K1A-regulated poly(A) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034388">
        <property type="entry name" value="Star-PAP_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF127">
        <property type="entry name" value="SPECKLE TARGETED PIP5K1A-REGULATED POLY(A) POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18 27" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="29">
        <location>
            <begin position="56"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="252"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="636"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="871" mass="93798" checksum="BB5BDA20EB78F803" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVJ9</accession>
    <name>A0A4W2BVJ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transmembrane protein 141</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TMEM141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027412698.1">
        <property type="nucleotide sequence ID" value="XM_027556897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVJ9"/>
    <dbReference type="SMR" id="A0A4W2BVJ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010166.1">
        <property type="protein sequence ID" value="ENSBIXP00000003277.1"/>
        <property type="gene ID" value="ENSBIXG00000009694.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054025.1">
        <property type="protein sequence ID" value="ENSBIXP00005027024.1"/>
        <property type="gene ID" value="ENSBIXG00005029089.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901873"/>
    <dbReference type="CTD" id="85014"/>
    <dbReference type="GeneTree" id="ENSGT00940000153116"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3350.20">
        <property type="entry name" value="Tmem141 protein family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026788">
        <property type="entry name" value="Tmem141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038259">
        <property type="entry name" value="Tmem141_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47229">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47229:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15110">
        <property type="entry name" value="TMEM141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="108" mass="11785" checksum="431EB64678F5FBEA" modified="2019-09-18" version="1">MVNLGLSRVDDAVASKHPGLGEYAACQSNAFVKGVSTFVTGTGATFGLQMLVQRKLPYPFQWKVLLAVVAGSVASYWVTRVESQKCSNLWLFLETGQLPKDMGTDRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVY9</accession>
    <name>A0A4W2BVY9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="12">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">USP2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase C19 family. USP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027419279.1">
        <property type="nucleotide sequence ID" value="XM_027563478.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027419280.1">
        <property type="nucleotide sequence ID" value="XM_027563479.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027419281.1">
        <property type="nucleotide sequence ID" value="XM_027563480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVY9"/>
    <dbReference type="SMR" id="A0A4W2BVY9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009892.1">
        <property type="protein sequence ID" value="ENSBIXP00000003413.1"/>
        <property type="gene ID" value="ENSBIXG00000009854.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042165.1">
        <property type="protein sequence ID" value="ENSBIXP00005026136.1"/>
        <property type="gene ID" value="ENSBIXG00005028585.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905752"/>
    <dbReference type="CTD" id="9099"/>
    <dbReference type="GeneTree" id="ENSGT00940000161289"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000024">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="268"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="53"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="606" mass="68101" checksum="4713919E8BDEE909" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWW6</accession>
    <name>A0A4W2BWW6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BWW6"/>
    <dbReference type="STRING" id="30522.A0A4W2BWW6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009231.1">
        <property type="protein sequence ID" value="ENSBIXP00000003762.1"/>
        <property type="gene ID" value="ENSBIXG00000010151.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000052692.1">
        <property type="protein sequence ID" value="ENSBIXP00000044007.1"/>
        <property type="gene ID" value="ENSBIXG00000005912.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008601.1">
        <property type="protein sequence ID" value="ENSBIXP00005004301.1"/>
        <property type="gene ID" value="ENSBIXG00005010256.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155186"/>
    <dbReference type="OMA" id="DTHRTGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051523">
        <property type="entry name" value="KISH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13229">
        <property type="entry name" value="PROTEIN KISH-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="60" mass="6783" checksum="14C3ED6C902E5191" modified="2019-09-18" version="1">MSAIFNFQSLLAVILLLICTYAYIRFLAPSLLDRNKTGLLGIFWKCARIGERKSPYVAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYM8</accession>
    <name>A0A4W2BYM8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hepatocyte growth factor-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Macrophage stimulatory protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MST1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts (via beta chain) with MST1R (via SEMA domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase S1 family. Plasminogen subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYM8"/>
    <dbReference type="STRING" id="30522.A0A4W2BYM8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008147.1">
        <property type="protein sequence ID" value="ENSBIXP00000004286.1"/>
        <property type="gene ID" value="ENSBIXG00000007460.1"/>
    </dbReference>
    <dbReference type="OMA" id="YQIPRCT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000002">
        <property type="entry name" value="Hepatocyte growth factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000004">
        <property type="entry name" value="Hepatocyte growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000055">
        <property type="entry name" value="Hepatocyte growth factor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000009">
        <property type="entry name" value="Hepatocyte growth factor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000004">
        <property type="entry name" value="Hepatocyte growth factor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000064">
        <property type="entry name" value="Hepatocyte growth factor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024174">
        <property type="entry name" value="HGF/MST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043575">
        <property type="entry name" value="MSP_HGFL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050759">
        <property type="entry name" value="Serine_protease_kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261:SF12">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261">
        <property type="entry name" value="PLASMINOGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
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    <sequence length="725" mass="81879" checksum="2D684881D27F1A45" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C396</accession>
    <name>A0A4W2C396_BOBOX</name>
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        <recommendedName>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <text evidence="2">Putative odorant or sperm cell receptor.</text>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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            <end position="290"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="35840" checksum="39804C8B6583160F" modified="2019-09-18" version="1">MANLTSVSGFLLVGFSADRKLQILHALLFLMTYLLALTGNLLIITITTLDHRLHSPMYYFLKHLSFLDLCFISVTVPQAIANSLMNNGYISLDQCILQVFFFIALASSEVAILTVMSYDRYVAICQPLQYETIMDPCACRHAVIAAWVAGGLSGLMHAAVNFSIPLCGDKVIHQFFCDVPQMLKLACSYEFINEIAVAAFTTSTAFICLISIVLSYIRIFSAVLRIPSAEGRTKVFSTCLPHLFVVTFFLSAAGFEFLRPPSDSQSAMDLMFSIFYTVIPPTLNPVIYSLRNEAMKAALRKTLSKEEFTQRKMHLKAIFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5Z3</accession>
    <name>A0A4W2C5Z3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable chloride channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:66338"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the tweety family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5Z3"/>
    <dbReference type="STRING" id="30522.A0A4W2C5Z3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043523.1">
        <property type="protein sequence ID" value="ENSBIXP00000008206.1"/>
        <property type="gene ID" value="ENSBIXG00000014113.1"/>
    </dbReference>
    <dbReference type="OMA" id="HTWQHKR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07912">
        <property type="entry name" value="Tweety_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF4">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-0868">Chloride</keyword>
    <keyword evidence="9 14" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="694"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="569"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="719"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="790"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="807"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="830" mass="88376" checksum="604B3C0F17F55F44" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C660</accession>
    <name>A0A4W2C660_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Forkhead box protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FOXL1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ESR1. Interacts with UBE2I/UBC9. Interacts with SMAD3. Interacts with DDX20.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027369664.1">
        <property type="nucleotide sequence ID" value="XM_027513863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C660"/>
    <dbReference type="SMR" id="A0A4W2C660"/>
    <dbReference type="STRING" id="30522.A0A4W2C660"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015885.1">
        <property type="protein sequence ID" value="ENSBIXP00000008272.1"/>
        <property type="gene ID" value="ENSBIXG00000014151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875450"/>
    <dbReference type="CTD" id="2300"/>
    <dbReference type="OMA" id="SCFPLHF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20027">
        <property type="entry name" value="FH_FOXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047514">
        <property type="entry name" value="FH_FOXL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF204">
        <property type="entry name" value="FORKHEAD BOX PROTEIN L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="11">
        <location>
            <begin position="49"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="9">
        <location>
            <begin position="49"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="136"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="346" mass="37363" checksum="529F35D14177D03C" modified="2019-09-18" version="1">MSHLFSPRLPALATSPMLYLYGPERPGLPLAFAPAGALAASGRVEPPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGGPEAKRVRAETQERGAEAPPEVQSAGGRPRPATPIQERAPARPSPLREASSPPPASLPWPRPGSPEGDAGDTLQGAAAVAVGQPERTVDGRGTPPRPASRSSPKSSDKSKSFSIDSILARRQGQKPTGGGELLGVAKQGPGGCLGASLLATSSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQLPEAVLHFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8N7</accession>
    <name>A0A4W2C8N7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase AMFR</fullName>
            <ecNumber evidence="12">2.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Autocrine motility factor receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.36" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C8N7"/>
    <dbReference type="STRING" id="30522.A0A4W2C8N7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044011.1">
        <property type="protein sequence ID" value="ENSBIXP00000009218.1"/>
        <property type="gene ID" value="ENSBIXG00000014935.1"/>
    </dbReference>
    <dbReference type="OMA" id="EPFCIWT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14421">
        <property type="entry name" value="CUE_AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16455">
        <property type="entry name" value="RING-H2_AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000149">
        <property type="entry name" value="E3 ubiquitin-protein ligase AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040675">
        <property type="entry name" value="AMFR_Ube2g2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18442">
        <property type="entry name" value="G2BR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="456"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="19">
        <location>
            <begin position="571"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="619"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="712"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="679"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="723"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="758" mass="84030" checksum="41CA7157A76070A6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C946</accession>
    <name>A0A4W2C946_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">3'-5' RNA helicase YTHDC2</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability. Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs. Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease. Required for both spermatogenesis and oogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 29">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MEIOC; binds transcripts that regulate the mitotic cell cycle inhibiting progression into metaphase, thereby allowing meiotic prophase to proceed normally. Interacts (via ANK repeats) with XRN1. Interacts with ZCCHC4. Associates with the small ribosomal subunit. Interacts with RBM46.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="SMR" id="A0A4W2C946"/>
    <dbReference type="STRING" id="30522.A0A4W2C946"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015615.1">
        <property type="protein sequence ID" value="ENSBIXP00000008102.1"/>
        <property type="gene ID" value="ENSBIXG00000014004.1"/>
    </dbReference>
    <dbReference type="OMA" id="EWIKRAN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
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    <keyword evidence="10" id="KW-0347">Helicase</keyword>
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    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0744">Spermatogenesis</keyword>
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        <location>
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            <end position="105"/>
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        <location>
            <begin position="202"/>
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        <location>
            <begin position="505"/>
            <end position="537"/>
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        <location>
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        <location>
            <begin position="1287"/>
            <end position="1417"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="1163"/>
            <end position="1287"/>
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        <location>
            <begin position="15"/>
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            <begin position="1163"/>
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            <begin position="1190"/>
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            <begin position="1230"/>
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        <location>
            <begin position="1249"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1429" mass="160297" checksum="9AB60BF2CEE70469" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9W6</accession>
    <name>A0A4W2C9W6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8 9">Stanniocalcin-2</fullName>
            <shortName evidence="9">STC-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">STC2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 9">Has an anti-hypocalcemic action on calcium and phosphate homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the stanniocalcin family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00005018970.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000005989"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046885">
        <property type="term" value="P:regulation of hormone biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004978">
        <property type="entry name" value="Stanniocalcin"/>
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    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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            <begin position="25"/>
            <end position="302"/>
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            <begin position="25"/>
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            <begin position="218"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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            <begin position="26"/>
            <end position="44"/>
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        <location>
            <begin position="227"/>
            <end position="264"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="302" mass="33193" checksum="035FB236F9D164EE" modified="2019-09-18" version="1">MCAERLGQFVTLALVLVTFDPARGTDATNTPEGPQDRGSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFSCISRKCPAIKEMVFQLQRECYLKHDLCSAAQENIRVMVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQAQCEQSWGSLCSILSFCTSAIQRPPTAPPEHQLQGDRAKLSRGHPAEMGHHLAEPSSRETGRGAKGERGSKSHPNAHARGRAAGPGAQGTSGSSEWEDEQSEYSDIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI92</accession>
    <name>A0A4W2CI92_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial</fullName>
            <ecNumber evidence="4">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for catalysing the entry and efficient transfer of electrons within complex I. Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 26">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000045771.1">
        <property type="protein sequence ID" value="ENSBIXP00000012811.1"/>
        <property type="gene ID" value="ENSBIXG00000017858.1"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
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    </dbReference>
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        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
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        <property type="entry name" value="Mopterin_OxRdtase"/>
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        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
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    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
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    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
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    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="17" id="KW-0408">Iron</keyword>
    <keyword evidence="18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="20" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="27">
        <location>
            <begin position="30"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="29">
        <location>
            <begin position="108"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="28">
        <location>
            <begin position="245"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00045300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="727" mass="79442" checksum="BEAD8AB5D76FC22D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLG4</accession>
    <name>A0A4W2CLG4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Laminin subunit alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Laminin M chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Laminin-12 subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Laminin-2 subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Laminin-4 subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Merosin heavy chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LAMA2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (laminin-211 or merosin), laminin-4 (laminin-221 or S-merosin) and laminin-12 (laminin-213). Interacts with FBLN1, FBLN2 and NID2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CLG4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022618.1">
        <property type="protein sequence ID" value="ENSBIXP00000012616.1"/>
        <property type="gene ID" value="ENSBIXG00000017773.1"/>
    </dbReference>
    <dbReference type="OMA" id="SHSRINF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000106">
        <property type="entry name" value="Heparan sulfate proteoglycan 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000074">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000069">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000082">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000242">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000512">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000189">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000250">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000315">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000008">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000052">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000057">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000061">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000063">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000065">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000017">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000051">
        <property type="entry name" value="Laminin subunit alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000094">
        <property type="entry name" value="Laminin subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000128">
        <property type="entry name" value="laminin subunit alpha-2 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000461">
        <property type="entry name" value="laminin subunit alpha-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000026">
        <property type="entry name" value="laminin subunit alpha-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009254">
        <property type="entry name" value="Laminin_aI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010307">
        <property type="entry name" value="Laminin_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF291">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06008">
        <property type="entry name" value="Laminin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06009">
        <property type="entry name" value="Laminin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9 22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 21" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021480005" description="Laminin subunit alpha-2" evidence="23">
        <location>
            <begin position="23"/>
            <end position="3122"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="27">
        <location>
            <begin position="35"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="414"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="469"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="26">
        <location>
            <begin position="538"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="757"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="807"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="865"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="918"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="967"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="1014"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="1060"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="1106"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="26">
        <location>
            <begin position="1180"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="1420"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="1527"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="24">
        <location>
            <begin position="2145"/>
            <end position="2328"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="24">
        <location>
            <begin position="2340"/>
            <end position="2521"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="24">
        <location>
            <begin position="2526"/>
            <end position="2710"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="24">
        <location>
            <begin position="2763"/>
            <end position="2934"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="24">
        <location>
            <begin position="2939"/>
            <end position="3119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="1682"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="2034"/>
            <end position="2140"/>
        </location>
    </feature>
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        </location>
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    <feature type="disulfide bond" evidence="21">
        <location>
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    <feature type="disulfide bond" evidence="21">
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            <begin position="2683"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00064740"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00083678"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50027"/>
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    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
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    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="3122" mass="343395" checksum="EC0A1D3700C32671" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLH5</accession>
    <name>A0A4W2CLH5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="13">Amino acid transporter heavy chain SLC3A1</fullName>
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            <fullName evidence="16">Neutral and basic amino acid transport protein</fullName>
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        <alternativeName>
            <fullName evidence="15">Solute carrier family 3 member 1</fullName>
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        <alternativeName>
            <fullName evidence="14">b(0,+)-type amino acid transporter-related heavy chain</fullName>
        </alternativeName>
    </protein>
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        <name evidence="20" type="primary">SLC3A1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Disulfide-linked heterodimer composed of the catalytic light subunit SLC7A9 and the heavy subunit SLC3A1. The heterodimer is the minimal functional unit. Assembles in non-covalently linked heterotetramers (dimers of heterodimers) and higher order oligomers; the oligomerization is mediated by SLC3A1 likely to prevent degradation in the endoplasmic reticulum and facilitate heteromer trafficking to the plasma membrane. Disulfide-linked heterodimer composed of the catalytic light subunit SLC7A13 and the heavy subunit SLC3A1.</text>
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        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSBIXP00000012626.1"/>
        <property type="gene ID" value="ENSBIXG00000017693.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005019282.1"/>
        <property type="gene ID" value="ENSBIXG00005022554.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000158103"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:aspartate transmembrane transport"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:gene expression"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:L-cystine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Maltase A3, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 3 (amino acid transporter heavy chain), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
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    </dbReference>
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        <property type="entry name" value="GH"/>
    </dbReference>
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        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="19">
        <location>
            <begin position="125"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="685" mass="78653" checksum="3762ECBBF958AF56" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMM1</accession>
    <name>A0A4W2CMM1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">IQ motif and SEC7 domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IQSEC3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a guanine nucleotide exchange factor (GEF) for ARF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DLG1 and DLG4. Interacts with GPHN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRAG family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CMM1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001599.1">
        <property type="protein sequence ID" value="ENSBIXP00000007555.1"/>
        <property type="gene ID" value="ENSBIXG00000001366.1"/>
    </dbReference>
    <dbReference type="OMA" id="QALTCYA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13318">
        <property type="entry name" value="PH_IQSEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000001">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000001">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000096">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033742">
        <property type="entry name" value="IQSEC_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF318">
        <property type="entry name" value="IQ MOTIF AND SEC7 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16453">
        <property type="entry name" value="IQ_SEC7_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="SEC7" evidence="13">
        <location>
            <begin position="758"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="177"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="331"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="547"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="629"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1117"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1171"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1248"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1125"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1141"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1195"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1269"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1304" mass="139503" checksum="505E29DEC6FFB783" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CN59</accession>
    <name>A0A4W2CN59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor EC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TFEC</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MiT/TFE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CN59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045949.1">
        <property type="protein sequence ID" value="ENSBIXP00000013233.1"/>
        <property type="gene ID" value="ENSBIXG00000003142.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18925">
        <property type="entry name" value="bHLHzip_TFEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000003">
        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021802">
        <property type="entry name" value="MiT/TFE_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR EC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11851">
        <property type="entry name" value="DUF3371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="110"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="317" mass="35673" checksum="9C7886AA472007CE" modified="2019-09-18" version="1">MTLDHQILNQSFKRSHPPTPSSELLVQHGHPSPKSDTGLTGNPLTKLLTLGKEDDNTEWHVSGSILDVYSGEQEISPVNMGLTSASCPSSLPMKREITETDTRALAKERQKKDNHNLIERRRRYNINYRIKELGTLIPKSNDPDMRWNKGTILKASVEYIKWLQKEQQRAQELEHRQKKLEQANRRLLLRIQELEIQARAHGLPTLASLVTVDLGAHITKQTHLEQNSGDYCQQLVLSQGTSPELCDQAMAFSDPLSHFTDLSFSAALKEEQRLDNMLLDDTVSPFGTDPLLSAISPAVSKESSRRSSFSSEDGDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQN1</accession>
    <name>A0A4W2CQN1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQN1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024906.1">
        <property type="protein sequence ID" value="ENSBIXP00000014162.1"/>
        <property type="gene ID" value="ENSBIXG00000018914.1"/>
    </dbReference>
    <dbReference type="OMA" id="GHYCEMF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22609">
        <property type="entry name" value="Kunitz_TKDP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF373">
        <property type="entry name" value="SERINE PEPTIDASE INHIBITOR, KUNITZ TYPE, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="376"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="433" mass="47318" checksum="94B53B16096D1FD7" modified="2019-09-18" version="1">MILGIQFDNHTQEELPTLEIEYSTLSEEIKGVETIHKQGIEKRAVLPVVKGILGGVLKGVKIFGKGASILTGLAEIIKKALKGQVMISGIQFDNSTQEELPTLEIEYATLSEEITGVETMHKQGIEKRSVKGILSGVLKGVKIVGKGASILIGLAEIVKKALKGQVMISGIQFDNHTQEEMPTLQIEYSTLIEENKGVETSHKQRLEKRAVLPVVKGILGGVLKGVKIFGQGASVLTGLAEIIKKALKGQVMISGIQFDNHTQEELPTLKIEYSTLIEENKGVETSQKRVLEKRSVVHVVKSILGGVIKGTKIFGKGASILTGLAEIVKKALKGQVMISRIQFDNHMQEELPTLNMEYSTLNEENKVSPAFMPAFCVEPKFVGVCNASMTRYFYNAQTGHCELFVYSGCGGNENNFPTLEECMKTCYPKAQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXK7</accession>
    <name>A0A4W2CXK7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113900860</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXK7"/>
    <dbReference type="STRING" id="30522.A0A4W2CXK7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028592.1">
        <property type="protein sequence ID" value="ENSBIXP00000016570.1"/>
        <property type="gene ID" value="ENSBIXG00000003851.1"/>
    </dbReference>
    <dbReference type="OMA" id="IVIMSEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15235">
        <property type="entry name" value="7tmA_OR1A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35500" checksum="3CC55CC4B53FDF9E" modified="2019-09-18" version="1">MERLNQTSSVSEFILLGLSSRPQDQKPLFILFLIIYLVTITGNLLIVLAICSDPQLHTPMYFFLSVLSFTDICFTTTIVPRMLVNFLSHKTISYAGCLSQMYFIYALGNTDSCLLAVMAFDRYVAICDPFHYVTIMGHRRCVLLVAFSCSLPHFHSLLHVLLLNQLTFCDSNVIHHFLCDLSPLMKLSCSPTLINEIVIMTEGPVLLVTPFLFITFSYIRILIAILKIPSTSGKHKAFSTCGSHLTVVTLFYGSIFYVYLQPVSTYTVRDHVATIVYTVLSSMLNPFIYSLRNKDLKQSLRKLVGRRKPQTAPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYC8</accession>
    <name>A0A4W2CYC8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Forkhead box protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DUSP15</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with ESR1. Interacts with UBE2I/UBC9. Interacts with SMAD3. Interacts with DDX20.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYC8"/>
    <dbReference type="STRING" id="30522.A0A4W2CYC8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029187.1">
        <property type="protein sequence ID" value="ENSBIXP00000016962.1"/>
        <property type="gene ID" value="ENSBIXG00000021042.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
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    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
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    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="92"/>
            <end position="200"/>
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            <begin position="1"/>
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            <begin position="412"/>
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            <begin position="479"/>
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            <begin position="71"/>
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            <begin position="285"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <sequence length="628" mass="68197" checksum="8F015A0FF603A44D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0I2</accession>
    <name>A0A4W2D0I2_BOBOX</name>
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        <recommendedName>
            <fullName evidence="20">Synaptojanin-2</fullName>
            <ecNumber evidence="7">3.1.3.36</ecNumber>
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        <alternativeName>
            <fullName evidence="21">Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2</fullName>
        </alternativeName>
    </protein>
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        <name evidence="26" type="primary">SYNJ2</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.36"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000021676.1"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0045121">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:SH3 domain binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="IP5"/>
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        <property type="entry name" value="IPPc"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="SAC_dom"/>
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        <property type="entry name" value="SYNJ1/2_RRM"/>
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    <dbReference type="InterPro" id="IPR034973">
        <property type="entry name" value="SYNJ2_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF148">
        <property type="entry name" value="SYNAPTOJANIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1866"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01165">
        <property type="entry name" value="DUF1866"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 22" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="SAC" evidence="25">
        <location>
            <begin position="120"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="24">
        <location>
            <begin position="890"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1029"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1084"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1195"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1418"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="1102"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1125"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="1261"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="1299"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1309"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1377"/>
            <end position="1393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00056929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00077889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1487" mass="163690" checksum="05A698AE90509CA9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7P4</accession>
    <name>A0A4W2D7P4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Frizzled-6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">FZD6</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="22">Interacts with LMBR1L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7P4"/>
    <dbReference type="STRING" id="30522.A0A4W2D7P4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000014754.1"/>
        <property type="gene ID" value="ENSBIXG00000007154.1"/>
    </dbReference>
    <dbReference type="OMA" id="FLKHNNR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15032">
        <property type="entry name" value="7tmF_FZD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07450">
        <property type="entry name" value="CRD_FZ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000036">
        <property type="entry name" value="frizzled-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000014">
        <property type="entry name" value="frizzled-6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041770">
        <property type="entry name" value="FZ6_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026543">
        <property type="entry name" value="FZD6_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF75">
        <property type="entry name" value="FRIZZLED-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="17 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 26" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0807">Transducer</keyword>
    <keyword evidence="10 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021216489" description="Frizzled-6" evidence="26">
        <location>
            <begin position="19"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="27">
        <location>
            <begin position="19"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="28">
        <location>
            <begin position="195"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="588"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="654"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00062611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="714" mass="80310" checksum="F8D83F0325D8E284" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8L3</accession>
    <name>A0A4W2D8L3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8L3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034807.1">
        <property type="protein sequence ID" value="ENSBIXP00000020619.1"/>
        <property type="gene ID" value="ENSBIXG00000006315.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="184"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="471" mass="54472" checksum="DD96BAB11A2B16D6" modified="2019-09-18" version="1">MSQCMFVKPQPPKYYLVFQIAVKRREAKSERKKEKYKHLNAEFQRIARRDKKAFFSDQCKEIEENNKMGKTRDLFKKIRDTKGTFHAKMSSIKDRNGMDLKEAEDIKKRWKEYTEELYKKDLHDPDNHDGVITDLEPDILECEVKWALESITTNKASGGDRIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRPVFILIPKKGNAKECSNYHTIALISHASKVILKILQARRQQYVNRELPDVQAGFRKGRGSRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILQEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLHAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPVTSWEIDGETVSDFVFLGSKITADEIKDAAMKLKDGYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8U9</accession>
    <name>A0A4W2D8U9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc-finger CCCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8U9"/>
    <dbReference type="STRING" id="30522.A0A4W2D8U9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049567.1">
        <property type="protein sequence ID" value="ENSBIXP00000020708.1"/>
        <property type="gene ID" value="ENSBIXG00000004786.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DUF5571"/>
    </dbReference>
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        <property type="entry name" value="Znf-CCCH_3"/>
    </dbReference>
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        <property type="entry name" value="RIKEN CDNA 1700017N19 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17732">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15663">
        <property type="entry name" value="zf-CCCH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-finger CCCH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="415" mass="47324" checksum="9B73122BE6EE31A6" modified="2019-09-18" version="1">MEMQQNCSISCFWETQPLGCVKISCIFYHSKPRNINGLFLPPSSNIILQKETQEGIPPPTQSQEPLKPQENISRPIHHPLVLKTNFEEEEEEEGEQNDASSLWTKTPEEIEEKRAIKEMCYKSGEYYRVHTPSDISSSKSIASIVEKEIEKSLESGSELQEGDGLTVPTKFSLFERQGEIKASLDRKPRTDIAAFENGGGDCYVPQRIIFLGVDENEVLTEEKESTISKCSNAKDNKDSPHPKRSLTTRLVSTRHVLNATENISMKCREDLSSMNDVHPVMKPHFKGVKKRKWIYDEPKNFPDPGMQRAVQASNPKNKMSYHRNNKNKNSENASYIHVQRDAVRTVSLNAPPHGRPPHGSYNKADVNKEPKFKLCSDKYMSTSYNGSAWQKRIPFSKTYSKNEKIYTEPRRNGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9T4</accession>
    <name>A0A4W2D9T4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Testis expressed 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TEX46</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9T4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038043.1">
        <property type="protein sequence ID" value="ENSBIXP00000022769.1"/>
        <property type="gene ID" value="ENSBIXG00000025561.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055067.1">
        <property type="protein sequence ID" value="ENSBIXP00005000101.1"/>
        <property type="gene ID" value="ENSBIXG00005030685.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007769"/>
    <dbReference type="OMA" id="IVWNKMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038788">
        <property type="entry name" value="TEX46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39412">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39412:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17671">
        <property type="entry name" value="DUF5531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18816" checksum="9A06E46C2A133C37" modified="2019-09-18" version="1">MLGDLMSLFWSLQGILASSGTIGALMAWLISYKPALFGFLFLLLLLSNWLVKHELKRTLQEPQQEEAEQSRTCEAKTNGNSLNTKEVERLHACFALQDKILERLMFSEMKLKVLENQMFIVWNRMNHHKQSSRRRTFPGGKHRMRRRESLFSILSDCTSNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAY3</accession>
    <name>A0A4W2DAY3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GRK6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027402647.1">
        <property type="nucleotide sequence ID" value="XM_027546846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAY3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035976.1">
        <property type="protein sequence ID" value="ENSBIXP00000021336.1"/>
        <property type="gene ID" value="ENSBIXG00000024462.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051208.1">
        <property type="protein sequence ID" value="ENSBIXP00005041863.1"/>
        <property type="gene ID" value="ENSBIXG00005004269.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895532"/>
    <dbReference type="CTD" id="2870"/>
    <dbReference type="GeneTree" id="ENSGT00940000158544"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05630">
        <property type="entry name" value="STKc_GRK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF15">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="186"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="449"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="582" mass="66585" checksum="EF1EBA9FC2E85057" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBG0</accession>
    <name>A0A4W2DBG0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050023.1">
        <property type="protein sequence ID" value="ENSBIXP00000021519.1"/>
        <property type="gene ID" value="ENSBIXG00000004975.1"/>
    </dbReference>
    <dbReference type="OMA" id="FANDICL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="420" mass="48388" checksum="E4F26777C0266416" modified="2019-09-18" version="1">MSSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHNDMITHLEPDILECEVKWALESITMNKASGGDRIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALISHASKVMLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANISWIMEKAREFHKNIYFCFIDYAKAFDCVNHNQLWKILKEMGIPEHLICLLRHLYTCQEATVRTGHRTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEETEKVGLKLNIQKTKIMASSPTTSWEIDGETVETVSEFIFLGSKITTDGDCSHEIKRRLLLGRKVMTNLDSILESRDITLPTKVRLVKAMVFPVVMYGCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DC81</accession>
    <name>A0A4W2DC81_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 17">Hyaluronidase</fullName>
            <ecNumber evidence="13 17">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13 17">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13 17">Belongs to the glycosyl hydrolase 56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="13 17"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DC81"/>
    <dbReference type="SMR" id="A0A4W2DC81"/>
    <dbReference type="STRING" id="30522.A0A4W2DC81"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028181.1">
        <property type="protein sequence ID" value="ENSBIXP00000016351.1"/>
        <property type="gene ID" value="ENSBIXG00000003780.1"/>
    </dbReference>
    <dbReference type="OMA" id="PFLWVWN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007342">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000065">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018155">
        <property type="entry name" value="Hyaluronidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001439">
        <property type="entry name" value="Hyaluronidase_PH20/Hyal5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769">
        <property type="entry name" value="HYALURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769:SF20">
        <property type="entry name" value="HYALURONIDASE PH-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01630">
        <property type="entry name" value="Glyco_hydro_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038193">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500773">
        <property type="entry name" value="Hyaluronidase_PH20_Hyal5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00846">
        <property type="entry name" value="GLHYDRLASE56"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00848">
        <property type="entry name" value="SPERMPH20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 13 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021225783" description="Hyaluronidase" evidence="19">
        <location>
            <begin position="36"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="477"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="15">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="60"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="383"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="439"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU610713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="553" mass="62281" checksum="63B68C2A38B6DB35" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DD45</accession>
    <name>A0A4W2DD45_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peroxisomal biogenesis factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peroxisomal assembly protein PEX3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in peroxisome biosynthesis and integrity. Assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, is necessary for the import of peroxisomal membrane proteins in the peroxisomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with PEX19.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DD45"/>
    <dbReference type="SMR" id="A0A4W2DD45"/>
    <dbReference type="STRING" id="30522.A0A4W2DD45"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028763.1">
        <property type="protein sequence ID" value="ENSBIXP00000016682.1"/>
        <property type="gene ID" value="ENSBIXG00000020798.1"/>
    </dbReference>
    <dbReference type="OMA" id="HRGWKDL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006966">
        <property type="entry name" value="Peroxin-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28080">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28080:SF1">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04882">
        <property type="entry name" value="Peroxin-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="373" mass="42103" checksum="2609F2D4944FA86E" modified="2019-09-18" version="1">MLRSTWNFLKRHKKKCIFLGTVLGGVYILGKYGQKKIREIQEREAAEYIAQARRQYHFESNQRTCNMTVLSMLPTLREALMQQLNSESLTALLKTRPSNKLEIWEDLKIISFTRSIVAVYSTCMLVVLLRVQLNIIGGYIYLDNAAVGKNGTTVLAPPDVQQQYLSSIQHLLGDGLTELITVIKQAVQKILGSVSLKHSLSLLDLEQKLKEIRDLVEQHKSSSWINNDGSKSLLCHYMMPDEETPLAVQACGLSPRDVTTIKLLNETRDMLESPDFSTVLNTCLSRGFSRLLDNMAEFFRPTEQDLQHGNSINSLSSVSLPLAKIIPIINGQIHSVCSETPSHFVQDLLMMEQVKDFAANVYEAFSTPQQLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDY9</accession>
    <name>A0A4W2DDY9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hyaluronan and proteoglycan link protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Brain link protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">HAPLN2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HAPLN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027385838.1">
        <property type="nucleotide sequence ID" value="XM_027530037.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385843.1">
        <property type="nucleotide sequence ID" value="XM_027530042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DDY9"/>
    <dbReference type="SMR" id="A0A4W2DDY9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040347.1">
        <property type="protein sequence ID" value="ENSBIXP00000024290.1"/>
        <property type="gene ID" value="ENSBIXG00000026502.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044501.1">
        <property type="protein sequence ID" value="ENSBIXP00005033308.1"/>
        <property type="gene ID" value="ENSBIXG00005014046.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884916"/>
    <dbReference type="CTD" id="60484"/>
    <dbReference type="GeneTree" id="ENSGT00940000161384"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033268">
        <property type="term" value="C:node of Ranvier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008065">
        <property type="term" value="P:establishment of blood-nerve barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085029">
        <property type="term" value="P:extracellular matrix assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03518">
        <property type="entry name" value="Link_domain_HAPLN_module_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03519">
        <property type="entry name" value="Link_domain_HAPLN_module_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000846">
        <property type="entry name" value="Hyaluronan and proteoglycan link protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000001">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF8">
        <property type="entry name" value="HYALURONAN AND PROTEOGLYCAN LINK PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610766" description="Hyaluronan and proteoglycan link protein 2" evidence="14">
        <location>
            <begin position="28"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="35"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="16">
        <location>
            <begin position="149"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="16">
        <location>
            <begin position="246"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="340" mass="37910" checksum="042BBDF31C23A34F" modified="2019-09-18" version="1">MQGRISLPTLCHFLLPWAFTTFHRALGSQAPHPGPHYLLPPIHEVIHSRRGATTTLPCVLGAPPPSYKVRWSKVEPGELRETPILITNGLHARGYGPLGGRARMRRGHRLDASLVIAGVRLEDEGRYRCELINGIEDESVAMTLRLEGVVFPYQPSRGRYQFNYYEAKQACEEQDGRLATYAQLYQAWTEGLDWCNAGWLLEGSVRYPVLTARAPCGGRGRPGIRSYGPRDRKRDRYDAFCFTSALSGHVFFVPGRLSLSEAHAACRRRGAMVAKVGHLYAAWKFSGLDQCDGGWLADGSVRFPITTPRPRCGGLPDPGVRSFGFPRPQQADYGTYCYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DF42</accession>
    <name>A0A4W2DF42_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rap1 GTPase-GDP dissociation stimulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RAP1GDS1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a GEF (guanine nucleotide exchange factor) for prenylated RHOA. Acts as a GEF for RHOC. Chaperones the downstream trafficking and/or processing of small newly prenylated GTPases. Escorts RAC1 to the nucleus.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Acts as a GEF (guanine nucleotide exchange factor) for the Rho family of small GTP-binding proteins (G proteins) that stimulates the dissociation of GDP to enable subsequent binding of GTP. Additionally, appears to chaperone the processing and/or trafficking of small GTPases containing a C-terminal polybasic region independently of GEF activity. Targets include RAP1A/RAP1B, RHOA, RHOB, RHOC, RAC1 and KRAS. Regulates mitochondrial dynamics by controlling RHOT function to promote mitochondrial fission during high calcium conditions. Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with RABL3. Interacts with RHOT1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with prenylated RHOA; the interaction is direct and in a 1:1 stoichiometry. Interacts with RAP1A. Interacts with KRAS. Interacts with RAC1. Interacts with RAP1B. Preferentially interacts with prenylated GTPases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with unprenylated RHOA; the interaction is direct. Interacts with RAP1A. Interacts with KRAS. Interacts with RAC1. Interacts with RAP1B. Preferentially interacts with unprenylated GTPases that will become geranylgeranylated. May also interact with prenylated GTPases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027399814.1">
        <property type="nucleotide sequence ID" value="XM_027544013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DF42"/>
    <dbReference type="SMR" id="A0A4W2DF42"/>
    <dbReference type="STRING" id="30522.A0A4W2DF42"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051807.1">
        <property type="protein sequence ID" value="ENSBIXP00000024794.1"/>
        <property type="gene ID" value="ENSBIXG00000026939.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027955.1">
        <property type="protein sequence ID" value="ENSBIXP00005016552.1"/>
        <property type="gene ID" value="ENSBIXG00005020210.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894113"/>
    <dbReference type="CTD" id="5910"/>
    <dbReference type="GeneTree" id="ENSGT00390000014293"/>
    <dbReference type="OMA" id="NGTEHQM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086098">
        <property type="term" value="P:angiotensin-activated signaling pathway involved in heart process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003300">
        <property type="term" value="P:cardiac muscle hypertrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032471">
        <property type="term" value="P:negative regulation of endoplasmic reticulum calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051561">
        <property type="term" value="P:positive regulation of mitochondrial calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070376">
        <property type="term" value="P:regulation of ERK5 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904464">
        <property type="term" value="P:regulation of matrix metallopeptidase secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000127">
        <property type="entry name" value="rap1 GTPase-GDP dissociation stimulator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000141">
        <property type="entry name" value="rap1 GTPase-GDP dissociation stimulator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000144">
        <property type="entry name" value="rap1 GTPase-GDP dissociation stimulator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="14">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="14">
        <location>
            <begin position="358"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="608" mass="66403" checksum="88AD6DC6EDD9B579" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGT7</accession>
    <name>A0A4W2DGT7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cyclin dependent kinase inhibitor 2A</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGT7"/>
    <dbReference type="STRING" id="30522.A0A4W2DGT7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030836.1">
        <property type="protein sequence ID" value="ENSBIXP00000018034.1"/>
        <property type="gene ID" value="ENSBIXG00000021932.1"/>
    </dbReference>
    <dbReference type="OMA" id="PNRYGRS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000107">
        <property type="entry name" value="cyclin-dependent kinase 4 inhibitor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF4">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="160" mass="16697" checksum="B8521FCE1BBAF68E" modified="2019-09-18" version="1">EGRALGSSMETSADLLAAAAALGWAEEVRALLEAGASANAPNRYGRSAIQVMMMGSARVAELLLLHGADPNCADPATLTRPVHDAAREGFLDTLVALHRAGAQLDVRDAWGRLPVDLAEERGHRDVARYLRAAAEDTEGGSHASADSAEGPADSSDLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DID0</accession>
    <name>A0A4W2DID0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SLC9A9</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399923.1">
        <property type="nucleotide sequence ID" value="XM_027544122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DID0"/>
    <dbReference type="SMR" id="A0A4W2DID0"/>
    <dbReference type="STRING" id="30522.A0A4W2DID0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039663.1">
        <property type="protein sequence ID" value="ENSBIXP00000023874.1"/>
        <property type="gene ID" value="ENSBIXG00000005447.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014402.1">
        <property type="protein sequence ID" value="ENSBIXP00005001353.1"/>
        <property type="gene ID" value="ENSBIXG00005007868.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894151"/>
    <dbReference type="CTD" id="285195"/>
    <dbReference type="GeneTree" id="ENSGT00940000160094"/>
    <dbReference type="OMA" id="FVKAMIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032009">
        <property type="term" value="C:early phagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090382">
        <property type="term" value="P:phagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002090">
        <property type="entry name" value="NHE-6/7/9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF61">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01088">
        <property type="entry name" value="NAHEXCHNGR6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="33"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="590"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="640" mass="72348" checksum="5C8031808CB3BE89" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJ61</accession>
    <name>A0A4W2DJ61_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NmrA-like family domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Interacts with ASS1. Interaction is enhanced by low NADPH/NADP(+) ratios, which results in inhibition of ASS1 activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJ61"/>
    <dbReference type="STRING" id="30522.A0A4W2DJ61"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053635.1">
        <property type="protein sequence ID" value="ENSBIXP00000026377.1"/>
        <property type="gene ID" value="ENSBIXG00000030140.1"/>
    </dbReference>
    <dbReference type="OMA" id="FMENTVA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05251">
        <property type="entry name" value="NmrA_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000181">
        <property type="entry name" value="NmrA-like family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF16">
        <property type="entry name" value="NMRA-LIKE FAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="10">
        <location>
            <begin position="149"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="444" mass="48418" checksum="1D7716DC70E43CDD" modified="2019-09-18" version="1">MQCISVRPDLRKFGLRRLRAGGSRPSAEFARVGTNHLCLRRGVACPLRAVRARTARIRKQPSLGSLHAGCLGKHGFARLPLELRARVGATCRVASPLCRGLGCSLTGPQFPERFTPRPPLGGTAGLEGPGKGCGLLGTTPSAPAHMADKKLVVVFGATGAQGGSVARTLLEDGTFRVRVVTRDPGQRAAKQLRLQGAEVVQGDQDDEASMELALSGAHATFIVTNYWENCSQEQEVKQGKLLADLAKRLGLRYVVYSGLENIKKLTAGRLTVGHFDGKGEVEEYFRDIGVPMTSVRLPCYFENLLSYFLPQKAPDGRSYLLSLPMGDVPIDGMSVADLGPVVLSLLKTPEEYVGRNIGLSTCRHTVEEYAALLTKHTGKAVRDAKTSPEDYEKLGFPGAQDLANMFRFYALKPDRNIELTLKLNPKARRLDQWLEQHKEDFAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJM4</accession>
    <name>A0A4W2DJM4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Phospholipid phosphatase 3</fullName>
            <ecNumber evidence="12">3.1.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="35">Lipid phosphate phosphohydrolase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="34">PAP2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Phosphatidate phosphohydrolase type 2b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Phosphatidic acid phosphatase 2b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">PLPP3</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="38 39 40" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Independently of this phosphatase activity may also function in the Wnt signaling pathway and the stabilization of beta-catenin/CTNNB1, thereby regulating cell proliferation, migration and differentiation in angiogenesis or yet in tumor growth. Also plays a role in integrin-mediated cell-cell adhesion in angiogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(9Z)-octadecenoyl-sn-glycero-3-phosphate + H2O = (9Z-octadecenoyl)-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:50884"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
            <dbReference type="ChEBI" id="CHEBI:84973"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:50885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43244"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:43245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O = 1,2-dihexadecanoyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43236"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:72859"/>
            <dbReference type="ChEBI" id="CHEBI:82929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:43237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>N-(9Z-octadecenoyl)-ethanolamine phosphate + H2O = N-(9Z-octadecenoyl) ethanolamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:62160"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
            <dbReference type="ChEBI" id="CHEBI:145465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:62161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-(octanoyl)-sphing-4-enine-1-phosphate + H2O = N-octanoylsphing-4-enine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:62040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45815"/>
            <dbReference type="ChEBI" id="CHEBI:85376"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:62041"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:27430"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a monoacyl-sn-glycero-3-phosphate + H2O = a monoacylglycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:46736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:77589"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:46737"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>an N-acylsphing-4-enine 1-phosphate + H2O = an N-acylsphing-4-enine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:33743"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57674"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:33744"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>sphing-4-enine 1-phosphate + H2O = sphing-4-enine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27518"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:60119"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:27519"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="24">Phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="30">Forms functional homodimers and homooligomers that are not required for substrate recognition and catalytic activity. Can also form heterooligomers with other PLPP2 and PLPP3. Interacts with CTNND1; negatively regulates the PLPP3-mediated stabilization of beta-catenin/CTNNB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Basolateral cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">trans-Golgi network membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane raft</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027392930.1">
        <property type="nucleotide sequence ID" value="XM_027537129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJM4"/>
    <dbReference type="STRING" id="30522.A0A4W2DJM4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040703.1">
        <property type="protein sequence ID" value="ENSBIXP00000024505.1"/>
        <property type="gene ID" value="ENSBIXG00000026646.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047209.1">
        <property type="protein sequence ID" value="ENSBIXP00005036849.1"/>
        <property type="gene ID" value="ENSBIXG00005018511.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889827"/>
    <dbReference type="CTD" id="8613"/>
    <dbReference type="GeneTree" id="ENSGT00940000156450"/>
    <dbReference type="OMA" id="YPYKRST"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106235">
        <property type="term" value="F:ceramide-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070097">
        <property type="term" value="F:delta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060020">
        <property type="term" value="P:Bergmann glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001568">
        <property type="term" value="P:blood vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033631">
        <property type="term" value="P:cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001702">
        <property type="term" value="P:gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010595">
        <property type="term" value="P:positive regulation of endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904906">
        <property type="term" value="P:positive regulation of endothelial cell-matrix adhesion via fibronectin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034112">
        <property type="term" value="P:positive regulation of homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902068">
        <property type="term" value="P:regulation of sphingolipid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030111">
        <property type="term" value="P:regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006670">
        <property type="term" value="P:sphingosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000013">
        <property type="entry name" value="Phospholipid phosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF79">
        <property type="entry name" value="PHOSPHOLIPID PHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21 36" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 36" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 36" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="37">
        <location>
            <begin position="131"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049314"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00065293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00074742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00075478"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00080459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00080707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00082411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="311" mass="35033" checksum="B365EECCA46CFE97" modified="2019-09-18" version="1">MQNYKYDKAIVAESKNGGSPALNNNPRKGGSKRVLLICLDLFCLFMAGLPFIIIETSTIKPYHRGFYCNDESIKYPQKTGETINDAVLTAVGIVIAILAIITGEFYRIYYLKEKSRSTIQNPYVAALYKQVGCFLFGCAISQSFTDIAKVSIGRLRPHFLNVCNPDFSQINCSVGYIQNYRCRGEDSKVQEARKSFFSGHASFSMYTMLYLVLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKTKTTLSLPPSAIRKDMLSPVDIDRSNHHNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJS1</accession>
    <name>A0A4W2DJS1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJS1"/>
    <dbReference type="STRING" id="30522.A0A4W2DJS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032601.1">
        <property type="protein sequence ID" value="ENSBIXP00000019126.1"/>
        <property type="gene ID" value="ENSBIXG00000022809.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="T cell receptor alpha variable 38-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF45">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="11">
        <location>
            <begin position="8"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="18"/>
            <end position="97"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12885" checksum="2C65436FC882A61A" modified="2019-09-18" version="1">AQKVTQDQPYVTSQIGQSVILNCWYEVSWSGYTHYLYWYKQLPSEEMTFLIRQESSGPNARNGRYSVNFQKAQNSISLTISALQLEDSAKYFFGCRQNHGRTVIHKDILEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKZ9</accession>
    <name>A0A4W2DKZ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTG anti-proliferation factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BTG3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402105.1">
        <property type="nucleotide sequence ID" value="XM_027546304.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402197.1">
        <property type="nucleotide sequence ID" value="XM_027546396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKZ9"/>
    <dbReference type="SMR" id="A0A4W2DKZ9"/>
    <dbReference type="STRING" id="30522.A0A4W2DKZ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051798.1">
        <property type="protein sequence ID" value="ENSBIXP00000024784.1"/>
        <property type="gene ID" value="ENSBIXG00000006153.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034561.1">
        <property type="protein sequence ID" value="ENSBIXP00005020967.1"/>
        <property type="gene ID" value="ENSBIXG00005023982.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895272"/>
    <dbReference type="CTD" id="10950"/>
    <dbReference type="GeneTree" id="ENSGT00950000182952"/>
    <dbReference type="OMA" id="HYGYRPR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.90:FF:000002">
        <property type="entry name" value="BTG anti-proliferation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033332">
        <property type="entry name" value="BTG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978">
        <property type="entry name" value="B-CELL TRANSLOCATION GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978:SF6">
        <property type="entry name" value="PROTEIN BTG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00960">
        <property type="entry name" value="BTG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01203">
        <property type="entry name" value="BTG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Anti-proliferative protein" evidence="3">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="252" mass="29132" checksum="B51983A3261AE01D" modified="2019-09-18" version="1">MKNEIAAVVFFFTRLVRKHDKLKKEAVERFAEKLTLILQEKYKNHWYPEKPSKGQAYRCIRVNKFQRVDPDVLKACENSCILYSDLGLPKELTLWVDPCEVCCRYGEKNNAFIVASFENEDENKDEISKKVTRALDKVTSDYHSGSSSSDEETCKEVEVKPNSVAATPSPVYQISELIFPPLPMWHPLPRKKPIMYRGNGNQSHYPPPIPFGYPNQGRKNKPYRPVPVTWVPPPGMHCDRNHWINPHMLAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMY3</accession>
    <name>A0A4W2DMY3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMY3"/>
    <dbReference type="STRING" id="30522.A0A4W2DMY3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042555.1">
        <property type="protein sequence ID" value="ENSBIXP00000025730.1"/>
        <property type="gene ID" value="ENSBIXG00000027829.1"/>
    </dbReference>
    <dbReference type="OMA" id="EIGHCND"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="108" mass="12207" checksum="038FB07220C33B8B" modified="2019-09-18" version="1">MKESKLGAGQAVSAVEGAVPSPPEAGRAPAPRPRVCRRSVFAGEYRFRFPGVAASRHSILKFFGHCNDLDREMRKCLKNEYMEKRNKSRELGNAMRKRLFNPPEESEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DPW2</accession>
    <name>A0A4W2DPW2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ARF GTPase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARF4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DPW2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053377.1">
        <property type="protein sequence ID" value="ENSBIXP00000026472.1"/>
        <property type="gene ID" value="ENSBIXG00000029969.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000098.1">
        <property type="protein sequence ID" value="ENSBIXP00005027703.1"/>
        <property type="gene ID" value="ENSBIXG00005030221.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156297"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001343">
        <property type="entry name" value="ADP-ribosylation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17716" checksum="FAE2E06C3227A214" modified="2019-09-18" version="1">MGLTISSLFSRLFGKKQMRILMGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEGAEELQKMLQEDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQF7</accession>
    <name>A0A4W2DQF7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Family with sequence similarity 240 member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FAM240A</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQF7"/>
    <dbReference type="STRING" id="30522.A0A4W2DQF7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010070.1">
        <property type="protein sequence ID" value="ENSBIXP00000028522.1"/>
        <property type="gene ID" value="ENSBIXG00000006774.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049572.1">
        <property type="protein sequence ID" value="ENSBIXP00005019058.1"/>
        <property type="gene ID" value="ENSBIXG00005003799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000069333"/>
    <dbReference type="OMA" id="IGKQTCY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040261">
        <property type="entry name" value="FAM240"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40387:SF3">
        <property type="entry name" value="PROTEIN FAM240A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40387">
        <property type="entry name" value="PROTEIN FAM240B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="78" mass="9725" checksum="177B517E1629B97A" modified="2019-09-18" version="1">MNNQYVRREVFCRNTCHELKRFWEREIGKQTYYRESEEHRLGRSALRKLREEWKQKVETKLRLRNNPDETEKQANVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQX4</accession>
    <name>A0A4W2DQX4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-1,4-glucuronyltransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">I-beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Poly-N-acetyllactosamine extension enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">B4GAT1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-O-[beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP-alpha-D-glucuronate = 3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46860"/>
            <dbReference type="Rhea" id="RHEA-COMP:15023"/>
            <dbReference type="Rhea" id="RHEA-COMP:17482"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:142405"/>
            <dbReference type="ChEBI" id="CHEBI:177336"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 49 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027387276.1">
        <property type="nucleotide sequence ID" value="XM_027531475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQX4"/>
    <dbReference type="SMR" id="A0A4W2DQX4"/>
    <dbReference type="STRING" id="30522.A0A4W2DQX4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009538.1">
        <property type="protein sequence ID" value="ENSBIXP00000028685.1"/>
        <property type="gene ID" value="ENSBIXG00000006611.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023788.1">
        <property type="protein sequence ID" value="ENSBIXP00005036336.1"/>
        <property type="gene ID" value="ENSBIXG00005017852.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885811"/>
    <dbReference type="CTD" id="11041"/>
    <dbReference type="GeneTree" id="ENSGT00940000157679"/>
    <dbReference type="OMA" id="SGQYRIY"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043189">
        <property type="entry name" value="B4GAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420:SF1">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="415" mass="47231" checksum="1B0FB74C8F8266C2" modified="2019-09-18" version="1">MQMSYAIRCAFYQLLLAALMLVAMLQLLYLSLLSGLHGQEEQDQYFEFFPPSPRSVDQVKAQLRTALASGGVLDASGDYRVYRGLLKTTMDPNDVILATHASVDNLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLTYALSSHCPDMRARVAMHLVCPSRYEAAVPDPREPGEFALLRSCQEVFDKLARVAQPGVNYALGTNVSYPNNLLRNLAREGANYALVIDVDMVPSEGLWRSLREMLDQSKQWAGTALVVPAFEIRRARRMPMNKNELLQLYQVGEVRPFYYGLCTPCQAPTNYSRWVNLPEETLLRPAYVVPWQDPWEPFYVAGGKVPTFDERFRQYGFNRISQACELHVAGFDFEVLNEGFLVHKGFKEVLKFHPQKEAENQHNKILYRQFKQELKAKYPDSPRHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRL4</accession>
    <name>A0A4W2DRL4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRL4"/>
    <dbReference type="STRING" id="30522.A0A4W2DRL4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015321.1">
        <property type="protein sequence ID" value="ENSBIXP00000026847.1"/>
        <property type="gene ID" value="ENSBIXG00000029300.1"/>
    </dbReference>
    <dbReference type="OMA" id="WIRNISK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015180">
        <property type="term" value="F:L-alanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015193">
        <property type="term" value="F:L-proline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF190">
        <property type="entry name" value="NEUTRAL AMINO ACID UNIPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="102"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="548" mass="60718" checksum="4059E08CAEC0EDE5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DS81</accession>
    <name>A0A4W2DS81_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DS81"/>
    <dbReference type="STRING" id="30522.A0A4W2DS81"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050200.1">
        <property type="protein sequence ID" value="ENSBIXP00000021880.1"/>
        <property type="gene ID" value="ENSBIXG00000005065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000016">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="14"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="112" mass="12536" checksum="86F4B4383B20DA48" modified="2019-09-18" version="1">TSDERDRNNGCSFKKCKCSRKESYSVYVYQVLKQVHLDSGISSKAMGIMNSFVNDVFEGISGEASCLVHYNKHMTITSRDIQTTRLMLLPGELAKHTMSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT35</accession>
    <name>A0A4W2DT35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Forkhead box protein O1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Forkhead box protein O1A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Forkhead in rhabdomyosarcoma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FOXO4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT35"/>
    <dbReference type="STRING" id="30522.A0A4W2DT35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006896.1">
        <property type="protein sequence ID" value="ENSBIXP00000029516.1"/>
        <property type="gene ID" value="ENSBIXG00000011177.1"/>
    </dbReference>
    <dbReference type="OMA" id="VHTEGHS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20062">
        <property type="entry name" value="FH_FOXO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000032">
        <property type="entry name" value="Forkhead box protein O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047409">
        <property type="entry name" value="FH_FOXO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032067">
        <property type="entry name" value="FOXO-TAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767:SF3">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16676">
        <property type="entry name" value="FOXO-TAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="13">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="11">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="182"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="511" mass="54237" checksum="FEE72CC3596D0B62" modified="2019-09-18" version="1">MDPVNENSAREAAAIVDLDPDFEPQSRPRSCTWPLPRPELAPEPSEASEVESGLGEKVHPEGRSDGRTQPTLLPARLPDSAGGPQPGILGAVTGPRKGGSRRNAWGNQSYAELISQAIESAPEKRLTLAQIYEWMVRTVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIKVHNEATGKSSWWMLNPEGGKSGKAPRRRAASMDSSSRLLRGRSKTPKKKPAVLPAPREGATPRSPAGHFAKWSGSPCSRNREEADAWSTFRPRSSSNASTVSTRPSPREPEPEVLAEEEMPASASGYAGGVPPTLKEDLELLDGLNLTSPHSLLSRSSLSGFSLQHPGVPGPLHTYSTSLFSPAEGPLSAGEGCFSSSQSLEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGPSSSKLGTGGGLCPKPLEAAGPSGLVPTLPMIAPAPPPVMGGAPVPKPLGAPVLTPPTEAPSQDRMPQDLDLDIYMENLECDMDNIINDLMDGDEGLDFNFEPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUX9</accession>
    <name>A0A4W2DUX9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Potassium voltage-gated channel subfamily C member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">KCNC1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027419217.1">
        <property type="nucleotide sequence ID" value="XM_027563416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUX9"/>
    <dbReference type="SMR" id="A0A4W2DUX9"/>
    <dbReference type="STRING" id="30522.A0A4W2DUX9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010760.1">
        <property type="protein sequence ID" value="ENSBIXP00000028291.1"/>
        <property type="gene ID" value="ENSBIXG00000009427.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003786.1">
        <property type="protein sequence ID" value="ENSBIXP00005031746.1"/>
        <property type="gene ID" value="ENSBIXG00005012180.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905722"/>
    <dbReference type="CTD" id="3746"/>
    <dbReference type="GeneTree" id="ENSGT00940000156912"/>
    <dbReference type="OMA" id="QTQSWAL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030673">
        <property type="term" value="C:axolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
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        <property type="term" value="F:delayed rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd18414">
        <property type="entry name" value="BTB_KCNC1_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Potassium channel, voltage-gated Shaw-related subfamily C, member 4"/>
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    <dbReference type="FunFam" id="1.20.120.350:FF:000014">
        <property type="entry name" value="Potassium channel, voltage-gated Shaw-related subfamily C, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily C member 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
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    </dbReference>
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        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
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        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
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        <property type="entry name" value="T1-type_BTB"/>
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        <property type="entry name" value="VG_K_chnl"/>
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        <property type="entry name" value="Volt_channel_dom_sf"/>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY C MEMBER 1"/>
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    </dbReference>
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        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01581">
        <property type="entry name" value="KV31CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="273"/>
            <end position="291"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="416"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="21">
        <location>
            <begin position="8"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="585" mass="65825" checksum="DCE52D32848E2DDE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXV1</accession>
    <name>A0A4W2DXV1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine dioxygenase</fullName>
            <ecNumber evidence="13">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CDO1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of cysteine to cysteine sulfinic acid with addition of molecular dioxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXV1"/>
    <dbReference type="SMR" id="A0A4W2DXV1"/>
    <dbReference type="STRING" id="30522.A0A4W2DXV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000678.1">
        <property type="protein sequence ID" value="ENSBIXP00000031570.1"/>
        <property type="gene ID" value="ENSBIXG00000001524.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027221.1">
        <property type="protein sequence ID" value="ENSBIXP00005016051.1"/>
        <property type="gene ID" value="ENSBIXG00005019797.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018226"/>
    <dbReference type="OMA" id="YTENQVT"/>
    <dbReference type="UniPathway" id="UPA00012">
        <property type="reaction ID" value="UER00537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000045">
        <property type="entry name" value="Cysteine dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0883">Thioether bond</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="11">
        <location>
            <begin position="163"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="270" mass="30708" checksum="C3496C4F1BEFC4F9" modified="2019-09-18" version="1">MGCQKAPCSGGERLRESHPKRWDRRGQRSARTRLPREPGSFAWALPAHVLAALSTLLSPAARRVPPVTRGMERTEVLKPRTLADLIRVLHQLFAGEEINVEEVQAVMEAYESNPAEWAVYAKFDQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKMLQGNLKETLFAWPDKKSNEMIKKSERILRENQCAYINDSIGLHRVENISHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVIMTFHSKFGIKTPFTTSGSLENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYE7</accession>
    <name>A0A4W2DYE7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED24</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYE7"/>
    <dbReference type="STRING" id="30522.A0A4W2DYE7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006807.1">
        <property type="protein sequence ID" value="ENSBIXP00000029544.1"/>
        <property type="gene ID" value="ENSBIXG00000011213.1"/>
    </dbReference>
    <dbReference type="OMA" id="RWSDSQW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021429">
        <property type="entry name" value="Mediator_Med24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
        <property type="entry name" value="Med24_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1018" mass="113516" checksum="CCD5DDF14C7CD506" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYL0</accession>
    <name>A0A4W2DYL0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYL0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008445.1">
        <property type="protein sequence ID" value="ENSBIXP00000029026.1"/>
        <property type="gene ID" value="ENSBIXG00000000607.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044845.1">
        <property type="protein sequence ID" value="ENSBIXP00005033749.1"/>
        <property type="gene ID" value="ENSBIXG00005002026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="366" mass="42230" checksum="2D26675210C28C46" modified="2019-09-18" version="1">MGSIKDRNGMNLTEAEDIKKRWQEYTELYKKDLHDQDNHDAVITHLESDILECEVKWALESITTNKASGGNGIPVELFQILKDDVVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSKYSTIALISHSSKVMLKMLQARLLQYVNREIPDVQAGFRKGRGMRDQTANIRWIMEKAREFQKNIYFSFIDYAKAFDCVDHNKLRKNLKEMGIPDHLTCFLRNLYAGQEATVRTGHGTIDWFQIGKGVRQGCISSPCLFNLYAEYIMRNARLEEAQAGIKTAGRNINNFRYADDTTLMAENEEELKSLLTKVKEDSEKVGLKLNIQKRKIMASGPITSWQIDGETGNTVRLYFSGLQNHCRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2Z9</accession>
    <name>A0A4W2E2Z9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic polyadenylation element binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CPEB3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384768.1">
        <property type="nucleotide sequence ID" value="XM_027528967.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384769.1">
        <property type="nucleotide sequence ID" value="XM_027528968.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384770.1">
        <property type="nucleotide sequence ID" value="XM_027528969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E2Z9"/>
    <dbReference type="STRING" id="30522.A0A4W2E2Z9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001977.1">
        <property type="protein sequence ID" value="ENSBIXP00000031150.1"/>
        <property type="gene ID" value="ENSBIXG00000001336.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884375"/>
    <dbReference type="CTD" id="22849"/>
    <dbReference type="OMA" id="CWAAIHA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008135">
        <property type="term" value="F:translation factor activity, RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12724">
        <property type="entry name" value="RRM1_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12726">
        <property type="entry name" value="RRM2_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000008">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.40:FF:000001">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000009">
        <property type="entry name" value="cytoplasmic polyadenylation element-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF7">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="457"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="565"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="714" mass="77929" checksum="F76986BC17C9961D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5U6</accession>
    <name>A0A4W2E5U6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC113905212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027417997.1">
        <property type="nucleotide sequence ID" value="XM_027562196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5U6"/>
    <dbReference type="STRING" id="30522.A0A4W2E5U6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022901.1">
        <property type="protein sequence ID" value="ENSBIXP00000034703.1"/>
        <property type="gene ID" value="ENSBIXG00000017753.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905212"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF121">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS, SUBFAMILY A, MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="193" mass="22067" checksum="DB5890AFF640766C" modified="2019-09-18" version="1">MPPDHLVLKKETRMLGAVQVMIGLINGALGRIWLLFYTRQFEELSVEYKPIALLSGYPFWTFLFFIISGVLTIQTEKKRSPNSVLTLFRRAGLWEGENQQTVECEEQGRVIKKIPNGKVRFVTVYASTTTDASGTDVSLFVQRRDDSGRGFESLRVQVRTAVFSQEQAKKNRDELDLLDMTESSLWVPRMPCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E630</accession>
    <name>A0A4W2E630_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ribosomal protein S6 kinase delta-1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">52 kDa ribosomal protein S6 kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RPS6KC1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell. Plays a role in the recruitment of PRDX3 to early endosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with SPHK1 and phosphatidylinositol 3-phosphate. Interacts (via PX domain) with PRDX3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027421421.1">
        <property type="nucleotide sequence ID" value="XM_027565620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E630"/>
    <dbReference type="SMR" id="A0A4W2E630"/>
    <dbReference type="STRING" id="30522.A0A4W2E630"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000672.1">
        <property type="protein sequence ID" value="ENSBIXP00000031566.1"/>
        <property type="gene ID" value="ENSBIXG00000013861.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036929.1">
        <property type="protein sequence ID" value="ENSBIXP00005022539.1"/>
        <property type="gene ID" value="ENSBIXG00005025469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906890"/>
    <dbReference type="CTD" id="26750"/>
    <dbReference type="GeneTree" id="ENSGT00940000155656"/>
    <dbReference type="OMA" id="GHSSELW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02677">
        <property type="entry name" value="MIT_SNX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07287">
        <property type="entry name" value="PX_RPK118_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05576">
        <property type="entry name" value="STKc_RPK118_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000221">
        <property type="entry name" value="ribosomal protein S6 kinase delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000005">
        <property type="entry name" value="ribosomal protein S6 kinase delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000017">
        <property type="entry name" value="ribosomal protein S6 kinase delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051866">
        <property type="entry name" value="Intracell_Sig-Traffick_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042132">
        <property type="entry name" value="PX_S6K-delta-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035053">
        <property type="entry name" value="STK_RPK118-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="1061" mass="118026" checksum="CA28AB5E16B2476F" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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            <dbReference type="Proteomes" id="UP000429181"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="156" mass="17488" checksum="F2A8EF7E13086D25" modified="2019-09-18" version="1">MWKRIDHQLKIKAGDGPQAGQFKELGPGREPAVPHPLSLSEFQTVPVFEDISQHVKEVGSQLVKKVNAIFQLDITKDGKTVHQWTIDLKNGSGDTYRGPARLPADTVFTIPEPVFMELILGKMNPQKAFLAGKFKVSGKVLLGQKLERVFKDWAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB80</accession>
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        <submittedName>
            <fullName evidence="11">G protein-coupled receptor 107</fullName>
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        <name evidence="11" type="primary">GPR107</name>
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    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EB80"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026648.1">
        <property type="protein sequence ID" value="ENSBIXP00000036374.1"/>
        <property type="gene ID" value="ENSBIXG00000019839.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054103">
        <property type="entry name" value="CAND6-7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF12">
        <property type="entry name" value="PROTEIN GPR107"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF21904">
        <property type="entry name" value="CAND6-7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021298833" evidence="8">
        <location>
            <begin position="40"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="CAND6/7 N-terminal" evidence="10">
        <location>
            <begin position="43"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="9">
        <location>
            <begin position="265"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="194"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="556" mass="62336" checksum="AEEDC8C329B3223A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECY0</accession>
    <name>A0A4W2ECY0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Tumor necrosis factor alpha-induced protein 3</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Putative DNA-binding protein A20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Zinc finger protein A20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">TNFAIP3</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 34">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C64 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECY0"/>
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    <dbReference type="STRING" id="30522.A0A4W2ECY0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028214.1">
        <property type="protein sequence ID" value="ENSBIXP00000037031.1"/>
        <property type="gene ID" value="ENSBIXG00000020552.1"/>
    </dbReference>
    <dbReference type="OMA" id="KCSGYCN"/>
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        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039532">
        <property type="term" value="P:negative regulation of cytoplasmic pattern recognition receptor signaling pathway"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032691">
        <property type="term" value="P:negative regulation of interleukin-1 beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035523">
        <property type="term" value="P:protein K29-linked deubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990168">
        <property type="term" value="P:protein K33-linked deubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002237">
        <property type="term" value="P:response to molecule of bacterial origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22766">
        <property type="entry name" value="OTU_TNFAIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4770:FF:000005">
        <property type="entry name" value="tumor necrosis factor alpha-induced protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000011">
        <property type="entry name" value="tumor necrosis factor alpha-induced protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.30:FF:000001">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.30:FF:000003">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.30:FF:000004">
        <property type="entry name" value="tumor necrosis factor alpha-induced protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.30:FF:000002">
        <property type="entry name" value="Tumor necrosis factor, alpha-induced protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF3">
        <property type="entry name" value="TUMOR NECROSIS FACTOR ALPHA-INDUCED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="22" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 28" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="30">
        <location>
            <begin position="92"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="31">
        <location>
            <begin position="381"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="31">
        <location>
            <begin position="467"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="31">
        <location>
            <begin position="591"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="31">
        <location>
            <begin position="641"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="31">
        <location>
            <begin position="746"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072722"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00077155"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00079762"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00451"/>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="780" mass="88203" checksum="E15BBCB965ECBE5C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEZ9</accession>
    <name>A0A4W2EEZ9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904800</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="STRING" id="30522.A0A4W2EEZ9"/>
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        <property type="gene ID" value="ENSBIXG00000017622.1"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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            <end position="51"/>
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        <location>
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        </location>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35623" checksum="1231923D9BDA39F8" modified="2019-09-18" version="1">MGGILYNSSELPTFTLTGLPGLENSQHWMFLLLGTLYIVSIVGNALILFIVKEEQSLHQPMYYFLSLLSVNDLGVSFSTLPTVLATFCFHLRKISFDSCMAQMFFIHFFSFMESGVLLVMSFDRYVAICNPLRYTTMLTDSRVVCMGLWVITRSFCMVFPLPFLLKRLSFCKANILSHAYCLHPDLIHLPCGDITVNNIFGLFIVVSTFGLDSALILFSYVLILHSVLAIASREERLKTFNTCVSHLCAVLIFYVPMVGVSMTARYGRHGPLLVHTVMSLIYLFVPPMLNPVIYSIKTKEIRRRLVRMLVGTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFD6</accession>
    <name>A0A4W2EFD6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFD6"/>
    <dbReference type="STRING" id="30522.A0A4W2EFD6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022994.1">
        <property type="protein sequence ID" value="ENSBIXP00000034826.1"/>
        <property type="gene ID" value="ENSBIXG00000017868.1"/>
    </dbReference>
    <dbReference type="OMA" id="MLCDFFK"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35016" checksum="D111A3F508D0BE29" modified="2019-09-18" version="1">MVSLPGFSLPQESHGALKAAERRELALGQNINLMTHFILLGFSEHPELQVILFSLFLGIFFMTLVWKLGLIVLIAVESHLHSPMYFFFGNLSFIDLSYTSSIAPKTLCDFFREQKTISLMGCAAQFFFFIGMGTRTLPALRSPPRSAQPSPLCAALPALRSPRDAPVCVRMAVATRSVSGLQFCGLRVIHHFFCDLPPLLVLSCFSPFLTLVVLVSYGYIMAAVTRIRSTPGWTKAFNTCASHLITVTLFYGPGLFAYLHSGVGYSPGQDMVVSLFCGVVSPKAEPHHIQFEKQGDKRCPEKTQGKKPMFTLCLIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFM5</accession>
    <name>A0A4W2EFM5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD finger protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PHF13</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response. Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis. Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation. Essential for testicular stem-cell differentiation and sustained spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027420831.1">
        <property type="nucleotide sequence ID" value="XM_027565030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFM5"/>
    <dbReference type="SMR" id="A0A4W2EFM5"/>
    <dbReference type="STRING" id="30522.A0A4W2EFM5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025591.1">
        <property type="protein sequence ID" value="ENSBIXP00000035948.1"/>
        <property type="gene ID" value="ENSBIXG00000019249.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023685.1">
        <property type="protein sequence ID" value="ENSBIXP00005036293.1"/>
        <property type="gene ID" value="ENSBIXG00005017804.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906627"/>
    <dbReference type="CTD" id="148479"/>
    <dbReference type="GeneTree" id="ENSGT00530000063882"/>
    <dbReference type="OMA" id="IPLHPQC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062072">
        <property type="term" value="F:histone H3K9me2/3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15632">
        <property type="entry name" value="PHD_PHF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000039">
        <property type="entry name" value="PHD finger protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041947">
        <property type="entry name" value="PHD_PHF13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571:SF13">
        <property type="entry name" value="PHD FINGER PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="14">
        <location>
            <begin position="234"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="300" mass="33589" checksum="685F93938F4FE1F6" modified="2019-09-18" version="1">MDSDSCAAAFHPEEYSPSYKRRRTVEDFNKFCTFVLAYAGYIPYPKEEVPLRSSPSPANSTAGTIDSDGWDASFADISSTVPLPVSDRCFGHLQPTLLQRAKPSNFLLDRKKTDKLKKKKKRKRRDSDVPGKEGYVGGLLQLEAADPYAETPTSPTLQDIPQAAGDPCSGWDSDTPSSGSCTTVSPDQVKEIKTEGKRTIVRQGKQVVFRDEDSTGNDEDIMVDSDDDSWDLVTCFCMKPFAGRPMIECNECHTWIHLSCAKIRKSNVPEVFVCQKCRDSKFDIRRSNRSRMGSRKLFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFV0</accession>
    <name>A0A4W2EFV0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Snake toxin/toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFV0"/>
    <dbReference type="STRING" id="30522.A0A4W2EFV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002608.1">
        <property type="protein sequence ID" value="ENSBIXP00000030935.1"/>
        <property type="gene ID" value="ENSBIXG00000001267.1"/>
    </dbReference>
    <dbReference type="OMA" id="PKSCCYT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000003">
        <property type="entry name" value="lymphocyte antigen 6E isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051110">
        <property type="entry name" value="Ly-6/neurotoxin-like_GPI-ap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16983">
        <property type="entry name" value="UPAR/LY6 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16983:SF16">
        <property type="entry name" value="UPAR_LY6 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021270827" description="Snake toxin/toxin-like domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Snake toxin/toxin-like" evidence="6">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="105" mass="11106" checksum="AF3256CEF523EFF0" modified="2019-09-18" version="1">MAVPRALLALLLAAASAVHLGEALSCITCEQPTALPLCKNITYCKPNEIACKTTLVTVEAEFPFNESPVVTSTCASSCEATDPDSIGAAHPIFCCFHDLCNSECW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGV6</accession>
    <name>A0A4W2EGV6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGV6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047192.1">
        <property type="protein sequence ID" value="ENSBIXP00000036728.1"/>
        <property type="gene ID" value="ENSBIXG00000020228.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043105.1">
        <property type="protein sequence ID" value="ENSBIXP00005045133.1"/>
        <property type="gene ID" value="ENSBIXG00005028939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="414" mass="47735" checksum="8A78D2D1241A8A71" modified="2019-09-18" version="1">MNIYCILLNYLTVMDRVKDLHLVNSKPDSLRSTKTQQWPQDWKRSVFIPIPKKDNAKECSNYRTIAIVSHASKVILKILQAKHQQYMNRELLDVQAGFRKGRGTRDQIAKSHWIIEKAREFQKNICFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEAIVRTGHGTTDWFQIGKEVRQGCIRSPCLFNLYTEYIMRNAGLEEAQAGIKIAGRNTNHLRYANDTTHMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVADFIFLGSKITADGDCSHEIKRSLLLGRKVMTNLDSILKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAEHQRIDAFELWCWRRLLRTARRSNQSILKEISPGSSLEGMTLKFKLQYFGHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHV4</accession>
    <name>A0A4W2EHV4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Terminal nucleotidyltransferase 4B</fullName>
            <ecNumber evidence="6">2.7.7.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Non-canonical poly(A) RNA polymerase PAPD5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">PAP-associated domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Terminal guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Terminal uridylyltransferase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Topoisomerase-related function protein 4-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">TENT4B</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35 36" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027369430.1">
        <property type="nucleotide sequence ID" value="XM_027513629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EHV4"/>
    <dbReference type="STRING" id="30522.A0A4W2EHV4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016812.1">
        <property type="protein sequence ID" value="ENSBIXP00000032165.1"/>
        <property type="gene ID" value="ENSBIXG00000007791.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016756.1">
        <property type="protein sequence ID" value="ENSBIXP00005008982.1"/>
        <property type="gene ID" value="ENSBIXG00005007491.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875294"/>
    <dbReference type="CTD" id="64282"/>
    <dbReference type="GeneTree" id="ENSGT00940000158301"/>
    <dbReference type="OMA" id="MYRAPRA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070568">
        <property type="term" value="F:guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060212">
        <property type="term" value="P:negative regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905870">
        <property type="term" value="P:positive regulation of 3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000015">
        <property type="entry name" value="Terminal nucleotidyltransferase 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF51">
        <property type="entry name" value="TERMINAL NUCLEOTIDYLTRANSFERASE 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="23" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="21" id="KW-0464">Manganese</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="20" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="33">
        <location>
            <begin position="213"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="32">
        <location>
            <begin position="401"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="103"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="563"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="31">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="31">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="617"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="639"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="655"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00076411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00079354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00081892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00082009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="712" mass="77596" checksum="A527B8B246908C83" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIZ8</accession>
    <name>A0A4W2EIZ8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">p53 apoptosis effector related to PMP-22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Keratinocyte-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PERP</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027407479.1">
        <property type="nucleotide sequence ID" value="XM_027551678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EIZ8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047428.1">
        <property type="protein sequence ID" value="ENSBIXP00000037037.1"/>
        <property type="gene ID" value="ENSBIXG00000020558.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038824.1">
        <property type="protein sequence ID" value="ENSBIXP00005042948.1"/>
        <property type="gene ID" value="ENSBIXG00005026496.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898478"/>
    <dbReference type="CTD" id="64065"/>
    <dbReference type="GeneTree" id="ENSGT00530000063484"/>
    <dbReference type="OMA" id="PTYFYSS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000014">
        <property type="entry name" value="p53 apoptosis effector related to PMP-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF4">
        <property type="entry name" value="P53 APOPTOSIS EFFECTOR RELATED TO PMP-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20641" checksum="4CE90907D087E704" modified="2019-09-18" version="1">MLRCGLACERCRWILPLLLLSAIVFDIIALAGRGWLQSSDDSQTSSLWWHCLLEVCDSLMTYAWGRAAAAMLLCGFIILVICFILSFFALCGPQMLVFLRVIGGLLALAAVFQIISLVIYPVKYTQTFNLHLNPAVTYIYNWAYGFGWAATIILIGCAFFFCCLPNYEDDLLGNAKPRYFYTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EL65</accession>
    <name>A0A4W2EL65_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centrosomal protein 152</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEP152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSBIXT00000025981.1">
        <property type="protein sequence ID" value="ENSBIXP00000036096.1"/>
        <property type="gene ID" value="ENSBIXG00000003470.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057659">
        <property type="entry name" value="CEP152_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057664">
        <property type="entry name" value="CEP152_PLK4_bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF6">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 152 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25770">
        <property type="entry name" value="CC_CEP63-bind_CEP152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25769">
        <property type="entry name" value="PLK4_bind_CEP152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEP152 CEP63 binding coiled coil" evidence="3">
        <location>
            <begin position="1276"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1389"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="244"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="623"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="742"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="989"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1250"/>
        </location>
    </feature>
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        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1399"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1658"/>
            <end position="1670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1725" mass="198519" checksum="7D49EE5A89BFE2A9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMR6</accession>
    <name>A0A4W2EMR6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030613.1">
        <property type="protein sequence ID" value="ENSBIXP00000038064.1"/>
        <property type="gene ID" value="ENSBIXG00000021793.1"/>
    </dbReference>
    <dbReference type="OMA" id="MEHGVIG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="68" mass="7729" checksum="809BE3067B29EEC1" modified="2025-10-08" version="2">MAVPNDLGWGSFWRTKCNTVAAMRVFAVGLWEGVEHKMEHGVIGGGFETFGFEKRGWRCGQNESRPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERZ6</accession>
    <name>A0A4W2ERZ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="37">Choline/ethanolaminephosphotransferase 1</fullName>
            <ecNumber evidence="34">2.7.8.1</ecNumber>
            <ecNumber evidence="35">2.7.8.2</ecNumber>
            <ecNumber evidence="36">2.7.8.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="38">1-alkenyl-2-acylglycerol choline phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="48" type="primary">CEPT1</name>
    </gene>
    <organism evidence="48 49">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="48 49 50" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="48" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="31">Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-choline = 1,2-di-(9Z-hexadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54336"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:83717"/>
            <dbReference type="ChEBI" id="CHEBI:84417"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54337"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-ethanolamine = 1,2-di-(9Z-hexadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:84417"/>
            <dbReference type="ChEBI" id="CHEBI:138145"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:54341"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1,2-didecanoyl-sn-glycerol + CDP-choline = 1,2-didecanoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18155"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78226"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,2-dioctanoyl-sn-glycerol + CDP-choline = 1,2-dioctanoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54232"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:76979"/>
            <dbReference type="ChEBI" id="CHEBI:78228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-O-alkyl-2-acyl-sn-glycerol + CDP-choline = a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="ChEBI" id="CHEBI:52595"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:36180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + CDP-choline = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54352"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55430"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77184"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:54353"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-O-hexadecyl-2-acetyl-sn-glycerol + CDP-choline = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54348"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:44811"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:75936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:54349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:82949"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:54245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-ethanolamine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54253"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>CDP-choline + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54240"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:54241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>CDP-choline + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:32940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>CDP-ethanolamine + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74986"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:54249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>CDP-ethanolamine + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32943"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.7.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:32944"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>a 1-O-(1Z-alkenyl)-2-acyl-sn-glycerol + CDP-choline = a 1-O-(1Z-alkenyl)-2-acyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77286"/>
            <dbReference type="ChEBI" id="CHEBI:77296"/>
            <dbReference type="EC" id="2.7.8.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:36228"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="32">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="33">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 45">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.1" evidence="34"/>
    <dbReference type="EC" id="2.7.8.2" evidence="35"/>
    <dbReference type="EC" id="2.7.8.22" evidence="36"/>
    <dbReference type="RefSeq" id="XP_027392012.1">
        <property type="nucleotide sequence ID" value="XM_027536211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ERZ6"/>
    <dbReference type="STRING" id="30522.A0A4W2ERZ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034293.1">
        <property type="protein sequence ID" value="ENSBIXP00000039704.1"/>
        <property type="gene ID" value="ENSBIXG00000023626.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045554.1">
        <property type="protein sequence ID" value="ENSBIXP00005011171.1"/>
        <property type="gene ID" value="ENSBIXG00005002434.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889483"/>
    <dbReference type="CTD" id="10390"/>
    <dbReference type="GeneTree" id="ENSGT00950000183117"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047359">
        <property type="term" value="F:1-alkenyl-2-acylglycerol choline phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004142">
        <property type="term" value="F:diacylglycerol cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004307">
        <property type="term" value="F:ethanolaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000002">
        <property type="entry name" value="Choline/ethanolamine phosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF27">
        <property type="entry name" value="CHOLINE_ETHANOLAMINEPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
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    <sequence length="416" mass="46450" checksum="76B421EC5BCB5BB6" modified="2019-09-18" version="1">MSGHRSTRKRCGDSHPESPLGFGHMSTPGCVLNKLFQLPTPPLSRHQLKRLEEHRYQSAGRSLLEPLMQGYWEWLVGRVPSWIAPNLITIIGLSINICTTVLLVFYCPTATEQAPLWAYIACACGLFIYQSLDAIDGKQARRTNSSSPLGELFDHGCDSLSTVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTYVSGTLRFGIIDVTEVQIFIIIMHLLAVIGGPPFWQSMIPVLNIQMKLFPALCTVAGTIFSCTNYFRVIFTGGVGKNGSTIAGTSVLSPFLHIGSVITLAAMIYKKSAVQLFEKHPCLYILTFGFVSAKITNKLVVAHMTKSEMHLHDTAFIGPALLFLDQYFNSFIDEYIVLWIALVFSFFDLIRYCVSVCNQIASHLHIHVFRIKVSTAHSNHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVV4</accession>
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            <fullName evidence="6">Profilin</fullName>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="2 6">Belongs to the profilin family.</text>
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    <sequence length="87" mass="9563" checksum="8C83D258235C869E" modified="2019-09-18" version="1">MTGQVFFVNGLTLGGQKCSVIRDSLLQDGEFTMDLRTKSTGGAPTFNITVTMTAKTLVLLMGKEGVHGGMINKKCYEMDSHLRRSQY</sequence>
</entry>
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    <accession>A0A4W2EXK9</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bickhart D.M."/>
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                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="32"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="486" mass="55635" checksum="AE0A924767D7DEAD" modified="2019-09-18" version="1">MNKASGGDGNPVELFQILKDDAVKVLHSICQQIWKTQQWPQDCKRSVFIPIPKKGNVKECSNYRTIAVISHASKVMFKILQARLQQYVNGELPDVQAAFRKGRGTRDQIANICWIMEKAREFQKNIYFCFIDYAKAFDCVNHKKLWKIVKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGATVETVSDFIFLGSKITDGDCSHEIKRCLLLVRKVMTNLDSILKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERQRIDAFELWCWRRLMRVPWTAKRSNQSILKEISPGISLGGMMLKLKLRYCGHLMRRVDSLDKTLMLGGIGGKRRRGRQRMRWLDGITDSMDVSLSELRELVMDREAWRAVIHEVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1T5</accession>
    <name>A0A4W2F1T5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTPase IMAP family member GIMD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GIMAP family P-loop NTPase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GIMD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399690.1">
        <property type="nucleotide sequence ID" value="XM_027543889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F1T5"/>
    <dbReference type="SMR" id="A0A4W2F1T5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041970.1">
        <property type="protein sequence ID" value="ENSBIXP00000043338.1"/>
        <property type="gene ID" value="ENSBIXG00000027428.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050844.1">
        <property type="protein sequence ID" value="ENSBIXP00005041382.1"/>
        <property type="gene ID" value="ENSBIXG00005024421.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894053"/>
    <dbReference type="CTD" id="100507096"/>
    <dbReference type="GeneTree" id="ENSGT00530000069080"/>
    <dbReference type="OMA" id="GKAMTDP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001392">
        <property type="entry name" value="GTPase IMAP family member GIMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF103">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER GIMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="6"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="217" mass="24765" checksum="D4D5DBC28B316BD0" modified="2019-09-18" version="1">MMNSNKMTINLALFGMTQSGKSSAGNIILGSTDFHSSFAPCSVTRDCSLGRSCHFRSFMRRGGQEVTLQVQVLDTPGYPHSRLSKKHVRQEVREALAHHFGQEGLHLALLVQRADVPLCGQEESSPVQMIQELLGHTWMNYTAILFTHAEKIEEAGFNEDEYLREASETLLKLLNSIQHRYIFQYKKGNSLSEQRLKILERIIEFVKENCYQVLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1Z1</accession>
    <name>A0A4W2F1Z1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acyl-CoA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DBI</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F1Z1"/>
    <dbReference type="STRING" id="30522.A0A4W2F1Z1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052190.1">
        <property type="protein sequence ID" value="ENSBIXP00000043406.1"/>
        <property type="gene ID" value="ENSBIXG00000027515.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005002583.1">
        <property type="protein sequence ID" value="ENSBIXP00005007337.1"/>
        <property type="gene ID" value="ENSBIXG00005012635.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154846"/>
    <dbReference type="OMA" id="WEGKKGM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032994">
        <property type="term" value="C:protein-lipid complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036042">
        <property type="term" value="F:long-chain fatty acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001140">
        <property type="term" value="P:positive regulation of phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF54">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ACB" evidence="10">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="97" mass="11080" checksum="A37E333EC9B22FD8" modified="2019-09-18" version="1">MSQVIRVPSLAAKAEFDKAAEEVKHLKTKPADEEMLFIYSHYKQATVGDINTERPGMLDFKGKAKWDAWNELKGTSKEDAMKAYIDKVEELKKKYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3B0</accession>
    <name>A0A4W2F3B0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3B0"/>
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        <property type="entry name" value="RT_nLTR_like"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="RT_dom"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="273"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="595" mass="67623" checksum="35A9E091363B3A32" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F4A4</accession>
    <name>A0A4W2F4A4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prostaglandin F2-alpha receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Prostanoid FP receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PTGFR</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="gene ID" value="ENSBIXG00000005900.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:prostaglandin F receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:inflammatory response"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:response to lipid"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="PglndnF_rcpt"/>
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        <property type="entry name" value="Prostglndn_DP_rcpt"/>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROSTAGLANDIN F2-ALPHA RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROSTAGLNDNR"/>
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        <property type="entry name" value="PROSTANOIDR"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
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        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
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        <location>
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            <end position="274"/>
        </location>
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            <begin position="43"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013486"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00030154"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="362" mass="41031" checksum="5E1AC4D696C3C13A" modified="2019-09-18" version="1">MSTNSSIQPVSPESELLSNTTCQLEEDLSISFSIIFMTVGILSNSLAIAILMKAYQRFRQKYKSSFLLLASALVITDFFGHLINGTIAVFVYASDKDWIYFDKSNILCSIFGICMVFSGLCPLFLGSLMAIERCIGVTKPIFHSTKITTKHVKMMLSGVCFFAVFVALLPILGHRDYKIQASRTWCFYKTDEIKDWEDRFYLLLFAFLGLLALGISFVCNAITGISLLKVKFRSQQHRQGRSHHFEMVIQLLGIMCVSCICWSPFLVTMASIGMNIQDFKDSCERTLFTLRMATWNQILDPWVYILLRKAVLRNLYVCTRRCCGVHVISLHVWELSSIKNSLKVAAISDLPVTEKVTQQTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F5I7</accession>
    <name>A0A4W2F5I7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase DZIP3</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase DZIP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DZIP3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A4W2F5I7"/>
    <dbReference type="STRING" id="30522.A0A4W2F5I7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043444.1">
        <property type="protein sequence ID" value="ENSBIXP00000044268.1"/>
        <property type="gene ID" value="ENSBIXG00000028439.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16460">
        <property type="entry name" value="RING-H2_DZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000308">
        <property type="entry name" value="E3 ubiquitin-protein ligase DZIP3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033103">
        <property type="entry name" value="DZIP3_RING-H2_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041249">
        <property type="entry name" value="HEPN_DZIP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056872">
        <property type="entry name" value="TTC3/DZIP3-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056870">
        <property type="entry name" value="TTC3/DZIP3/RBM44-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043866">
        <property type="entry name" value="TTC3/DZIP3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15727:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15727">
        <property type="entry name" value="RING FINGER PROTEIN 214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18738">
        <property type="entry name" value="HEPN_DZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24525">
        <property type="entry name" value="TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24905">
        <property type="entry name" value="TTC3_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19179">
        <property type="entry name" value="TTC3_DZIP3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="1145"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="642"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1086"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="805"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1088"/>
            <end position="1114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1205" mass="137869" checksum="7471DB0EE6975619" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FAQ3</accession>
    <name>A0A4W2FAQ3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Troponin C, slow skeletal and cardiac muscles</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TNNC1</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379039.1">
        <property type="nucleotide sequence ID" value="XM_027523238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FAQ3"/>
    <dbReference type="SMR" id="A0A4W2FAQ3"/>
    <dbReference type="STRING" id="30522.A0A4W2FAQ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053909.1">
        <property type="protein sequence ID" value="ENSBIXP00000026282.1"/>
        <property type="gene ID" value="ENSBIXG00000030313.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012355.1">
        <property type="protein sequence ID" value="ENSBIXP00005002386.1"/>
        <property type="gene ID" value="ENSBIXG00005008732.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880720"/>
    <dbReference type="CTD" id="7134"/>
    <dbReference type="GeneTree" id="ENSGT00940000153541"/>
    <dbReference type="OMA" id="QKSEFRA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990584">
        <property type="term" value="C:cardiac Troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031013">
        <property type="term" value="F:troponin I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031014">
        <property type="term" value="F:troponin T binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086003">
        <property type="term" value="P:cardiac muscle cell contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032972">
        <property type="term" value="P:regulation of muscle filament sliding speed"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014883">
        <property type="term" value="P:transition between fast and slow fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055010">
        <property type="term" value="P:ventricular cardiac muscle tissue morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000033">
        <property type="entry name" value="Troponin C, slow skeletal and cardiac muscles"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF47">
        <property type="entry name" value="TROPONIN C1, SLOW SKELETAL AND CARDIAC TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="161" mass="18417" checksum="5FB0BC46D503A243" modified="2019-09-18" version="1">MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLEELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FS49</accession>
    <name>A0A4W2FS49_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribosomal protein S6 kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RPS6KA2</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027407826.1">
        <property type="nucleotide sequence ID" value="XM_027552025.1"/>
    </dbReference>
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    <sequence length="733" mass="83106" checksum="C847C957F9162487" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 9" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="111"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="281"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="376" mass="41912" checksum="062DA89F5B7FE6A6" modified="2019-09-18" version="1">MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNIKDTLGLGRTRRKTSARDASPTPSTDAEFPANGGGADRIYDLSIPAVVKFAYAAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQVGWFPSNYVLEELDEAAGDAPGGSSLRPGATLSNGQGARALQVVQTLYPFSSVTEEELNFDKGETMEVIEKPENDPEWWRCRNARGQVGLVPKNYVVVLSEGPAPHAGSGPAGPARAGRFAGREWYYGNVTRHQAECALNARGVQGDFLVRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FVF4</accession>
    <name>A0A4W2FVF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein with KRAB and SCAN domains 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ZKSCAN5</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383482.1">
        <property type="nucleotide sequence ID" value="XM_027527681.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383483.1">
        <property type="nucleotide sequence ID" value="XM_027527682.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383484.1">
        <property type="nucleotide sequence ID" value="XM_027527683.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383485.1">
        <property type="nucleotide sequence ID" value="XM_027527684.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383486.1">
        <property type="nucleotide sequence ID" value="XM_027527685.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383488.1">
        <property type="nucleotide sequence ID" value="XM_027527687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FVF4"/>
    <dbReference type="STRING" id="30522.A0A4W2FVF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053413.1">
        <property type="protein sequence ID" value="ENSBIXP00000000291.1"/>
        <property type="gene ID" value="ENSBIXG00000029996.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017349.1">
        <property type="protein sequence ID" value="ENSBIXP00005009397.1"/>
        <property type="gene ID" value="ENSBIXG00005001885.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883739"/>
    <dbReference type="CTD" id="23660"/>
    <dbReference type="GeneTree" id="ENSGT00940000162299"/>
    <dbReference type="OMA" id="KSQRCND"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000139">
        <property type="entry name" value="zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000185">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein with KRAB and SCAN domains 5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001898">
        <property type="entry name" value="Zinc finger protein with KRAB and SCAN domains 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000642">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001643">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="18">
        <location>
            <begin position="51"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="19">
        <location>
            <begin position="218"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="550"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="578"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="662"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="718"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="746"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="802"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="832" mass="95670" checksum="E142C1ADB3CB5D59" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FWG5</accession>
    <name>A0A4W2FWG5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSBIXG00000016475.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005009782.1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="383" mass="43733" checksum="8FA7A137980266FA" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQAGFRKSRERNQKSNCQYPLDHGKSKSSRKTSISALLTMPKPLTVVDHNKLWKILKEMGIADHLTYLLRNLYASQEATVRTGHGTDWFQIGKGVRQGCILSACSFNLYAEYIMRNAGLEEAQARIKISGRNINNLRYADDTTLMAESEEELKSLLMKVKEENEKVGSELNIQKTKIMVSGPITSWEIDGETVETVSDFIVLGSKITADGDCSHEIKRHLLFGRKVMTNLDSILKSRDITLPTNVRLVKAMVFPVVTYGCESWTVKKAECRRIDGFELWCWRRLLKVPWTARRSNQSILKETSPGCSLEGLKLKLKLQYFGHLKRRVDSLEKTLMLGGIEGRKRRGQQDEMAGWHHRLDGHVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G7S7</accession>
    <name>A0A4W2G7S7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G7S7"/>
    <dbReference type="STRING" id="30522.A0A4W2G7S7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046424.1">
        <property type="protein sequence ID" value="ENSBIXP00000035683.1"/>
        <property type="gene ID" value="ENSBIXG00000018989.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024153.1">
        <property type="protein sequence ID" value="ENSBIXP00005013996.1"/>
        <property type="gene ID" value="ENSBIXG00005018056.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282777"/>
    <dbReference type="OMA" id="YLGRFWI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="90" mass="9829" checksum="DAE2F36CF7808FF3" modified="2019-09-18" version="1">MPGSALGLARRALSGRPFQTAPCSTHRALSRSAALRKVTKAQGGCPKSFVLWGCSPKRPRKRRTSLKLAWRGTFCSSSLKTSEVNRPARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GDW6</accession>
    <name>A0A4W2GDW6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS17m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">28S ribosomal protein S17, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GDW6"/>
    <dbReference type="SMR" id="A0A4W2GDW6"/>
    <dbReference type="STRING" id="30522.A0A4W2GDW6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000499.1">
        <property type="protein sequence ID" value="ENSBIXP00000031611.1"/>
        <property type="gene ID" value="ENSBIXG00000013929.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027899.1">
        <property type="protein sequence ID" value="ENSBIXP00005016514.1"/>
        <property type="gene ID" value="ENSBIXG00005020161.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064130"/>
    <dbReference type="OMA" id="KHFREND"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000137">
        <property type="entry name" value="28S ribosomal protein S17, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039193">
        <property type="entry name" value="Ribosomal_uS17m_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14446" checksum="E092F22CE2D89937" modified="2019-09-18" version="1">MSVVRSSVHAKWIVGKVIGTAMQKTAKVRVTRLVLDPYLLKYFNKQKTYFAHDALQQCTVGDIVLLKALPVPRTKHVKHELAEIVFKVGQVVDPVTGKRCAGTTYLESPVDLETTPLAKNLEELSLSTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GMC5</accession>
    <name>A0A4W2GMC5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neuromedin-K receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">NK-3 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Neurokinin B receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tachykinin receptor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TACR3</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399727.1">
        <property type="nucleotide sequence ID" value="XM_027543926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GMC5"/>
    <dbReference type="SMR" id="A0A4W2GMC5"/>
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    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
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            <begin position="335"/>
            <end position="357"/>
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        <location>
            <begin position="100"/>
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            <begin position="412"/>
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            <begin position="438"/>
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            <begin position="156"/>
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    <sequence length="463" mass="52061" checksum="0DEAFF1F9AA1624C" modified="2019-09-18" version="1">MASLPVAANWTNGGTAGADIGNLSALEPGAAAGKAETEWLQLLVQAGNLSASFSPLGLAAASPAPSQSRSNITNQFVQPSWRIALWSLAYGVVVAVAVFGNLIVIWIILAHKRMRTVTNYFLVNLAFSDASMAAFNTLVNFIYALHSEWYFGANYCRFQNFFPITAVFASIYSMTAISVDRYMAIIDPLKPRLSATATKIVIGGIWILAFLLALPQCLYSKTKVMPGRTLCYVQWPEGPKQHFTYHIIVIILVYCFPLLVMGITYTIVGITLWGGEIPGDTCDKYHEQLKAKRKVVKMMIIVVVTFAICWLPYHIYFILTAIYQQLNRWKYIQQVYLASFWLAMSSTMYNPIIYCCLNKRFRAGFKRAFRWCPFIEVSSYDELELKTTRFHPTRQSSLYTVTRMESMTVVFDPSDADNTRSSRKKRVPPRDPSFNGCSRQNSKSGSTTSSFISSPYTSVDEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GST1</accession>
    <name>A0A4W2GST1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Src substrate cortactin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">CTTN</name>
    </gene>
    <organism evidence="30 32">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31 32" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <location evidence="11">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027388756.1">
        <property type="nucleotide sequence ID" value="XM_027532955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GST1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007838.1">
        <property type="protein sequence ID" value="ENSBIXP00000029223.1"/>
        <property type="gene ID" value="ENSBIXG00000010796.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033645.1">
        <property type="protein sequence ID" value="ENSBIXP00005020333.1"/>
        <property type="gene ID" value="ENSBIXG00005023643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886595"/>
    <dbReference type="CTD" id="2017"/>
    <dbReference type="GeneTree" id="ENSGT00940000158565"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11959">
        <property type="entry name" value="SH3_Cortactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000087">
        <property type="entry name" value="Src substrate cortactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035716">
        <property type="entry name" value="Cortactin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003134">
        <property type="entry name" value="Hs1_Cortactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF15">
        <property type="entry name" value="SRC SUBSTRATE CORTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02218">
        <property type="entry name" value="HS1_rep"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51090">
        <property type="entry name" value="CORTACTIN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="25" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-0168">Coated pit</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 27" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="20" id="KW-0770">Synapse</keyword>
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        <location>
            <begin position="443"/>
            <end position="501"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
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        <location>
            <begin position="293"/>
            <end position="405"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="28">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="320"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00073863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="501" mass="56158" checksum="DEB53C0537ADBF88" modified="2019-09-18" version="1">MWKASAGHTVSITPDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQVDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQSDRVDKSAVGFDYQGETEKHGSQRDYSRGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKPQLHESQKDYSKGFGGKYGVQKDRMDKNASTFEDVAAVSSSYQKTVPVEAVNSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAQERQEQEAARRQLDEQARAQKPTPPASPTPQPAQERPPSSPVYEDAAPFRAEPSPSREPELGYSVETTEYPEAYVSEGVYESTEAPGHYRAEEGTYDEYENDLGITAIALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGLCKGRYGLFPANYVELRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GYW7</accession>
    <name>A0A4W2GYW7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thioredoxin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TXN2</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397564.1">
        <property type="nucleotide sequence ID" value="XM_027541763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GYW7"/>
    <dbReference type="SMR" id="A0A4W2GYW7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041081.1">
        <property type="protein sequence ID" value="ENSBIXP00000042892.1"/>
        <property type="gene ID" value="ENSBIXG00000026900.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037242.1">
        <property type="protein sequence ID" value="ENSBIXP00005022756.1"/>
        <property type="gene ID" value="ENSBIXG00005025598.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892649"/>
    <dbReference type="CTD" id="25828"/>
    <dbReference type="GeneTree" id="ENSGT00530000064086"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000001">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
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        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF3">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="51"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="166" mass="18416" checksum="D796F7C155EA5F4A" modified="2019-09-18" version="1">MAQRLLLRRFLTSIISGKPSQSRWAPVASRALQTPQYSPGYLTVTPSQARSIYTTRVCSTTFNIQDGPDFQDRVVNSETPVVVDFHAQWCGPCKILGPRLEKVVAKQHGKVVMAKVDIDDHTDLALEYEVSAVPTVLAMKNGDVVDKFVGIKDEDQLEAFLKKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H9R0</accession>
    <name>A0A4W2H9R0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Calcium channel flower domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CACFD1</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel flower family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H9R0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009709.1">
        <property type="protein sequence ID" value="ENSBIXP00000028631.1"/>
        <property type="gene ID" value="ENSBIXG00000009920.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015610.1">
        <property type="protein sequence ID" value="ENSBIXP00005027889.1"/>
        <property type="gene ID" value="ENSBIXG00005029903.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000529"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="130" mass="13639" checksum="68E0A1B172791459" modified="2019-09-18" version="1">MSGAGGAAVAPGSSAQPAQEEGMTWWYRWLCRLSGVLGAVSCAISGLFNCITIHPLNIAAGVWMVMAVVPIVISLTLTTLLGNAIAFATGVLYGLSALGKKGDAISYARIQQQRQQVDEEKLTDTLEGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H9S2</accession>
    <name>A0A4W2H9S2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-related protein Rab-43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RAB43</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Phagosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379303.1">
        <property type="nucleotide sequence ID" value="XM_027523502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H9S2"/>
    <dbReference type="SMR" id="A0A4W2H9S2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011625.1">
        <property type="protein sequence ID" value="ENSBIXP00000002586.1"/>
        <property type="gene ID" value="ENSBIXG00000009058.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042762.1">
        <property type="protein sequence ID" value="ENSBIXP00005026493.1"/>
        <property type="gene ID" value="ENSBIXG00005005001.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880874"/>
    <dbReference type="CTD" id="339122"/>
    <dbReference type="GeneTree" id="ENSGT00940000161980"/>
    <dbReference type="OMA" id="FIERHGN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071346">
        <property type="term" value="P:cellular response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090382">
        <property type="term" value="P:phagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035526">
        <property type="term" value="P:retrograde transport, plasma membrane to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019068">
        <property type="term" value="P:virion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01864">
        <property type="entry name" value="Rab19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000803">
        <property type="entry name" value="Ras-related protein Rab-43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048040">
        <property type="entry name" value="Rab19/43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="210" mass="23088" checksum="FA538E45931EC686" modified="2019-09-18" version="1">MAGPGPGDPDEQYDFLFKLVLVGDASVGKTCVVQRFKTGTFSERQGSTIGVDFTMKTLEIQGQRVKLQIWDTAGQERFRTITQSYYRSANGAILAYDITKRSSFLSVPHWIEDVRKYAGSNIVQLLIGNKSDLGQLREVPLAEAQGLAEHYDILCAIETSAKDSSNVEEAFVRVATELVVRHGGPRLSEKGADHIQLDSKDVAESWGCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HEE9</accession>
    <name>A0A4W2HEE9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lin-37 DREAM MuvB core complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LIN37</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027370167.1">
        <property type="nucleotide sequence ID" value="XM_027514366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HEE9"/>
    <dbReference type="SMR" id="A0A4W2HEE9"/>
    <dbReference type="STRING" id="30522.A0A4W2HEE9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019285.1">
        <property type="protein sequence ID" value="ENSBIXP00000033193.1"/>
        <property type="gene ID" value="ENSBIXG00000015932.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010444.1">
        <property type="protein sequence ID" value="ENSBIXP00005029566.1"/>
        <property type="gene ID" value="ENSBIXG00005009509.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875690"/>
    <dbReference type="CTD" id="55957"/>
    <dbReference type="GeneTree" id="ENSGT00390000002748"/>
    <dbReference type="OMA" id="SNVARWK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031523">
        <property type="term" value="C:Myb complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045023">
        <property type="term" value="P:G0 to G1 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028226">
        <property type="entry name" value="LIN37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336">
        <property type="entry name" value="LIN37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336:SF3">
        <property type="entry name" value="PROTEIN LIN-37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15306">
        <property type="entry name" value="LIN37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="246" mass="28480" checksum="32D9CD60EA95C424" modified="2019-09-18" version="1">MFPVKVKVEKSELEMAKARNQLDAVLQCLLEKSHMDRERLDEEPGKTSLDTHNKDCSITATGKRPSARFPHQRRKKRREMDEGLAEGGPQRSNTYVIKLFDRSVDLAQFSENTPLYPICRAWMRNSPTVRERERSPSSPLPPLPEDEEGSEVTNSKSRDVYKLPPPTAPGPPGDACRSRIPSPLQPETQGTPDDEPSEPEPSPSTLIYRNMQRWKRIRQRWKEASHRNQLRYSESMKILREMYERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HG55</accession>
    <name>A0A4W2HG55_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HG55"/>
    <dbReference type="STRING" id="30522.A0A4W2HG55"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004669.1">
        <property type="protein sequence ID" value="ENSBIXP00000005962.1"/>
        <property type="gene ID" value="ENSBIXG00000001117.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012834.1">
        <property type="protein sequence ID" value="ENSBIXP00005028782.1"/>
        <property type="gene ID" value="ENSBIXG00005008532.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111487"/>
    <dbReference type="OMA" id="NMTRNAW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="2">
        <location>
            <begin position="25"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17086" checksum="0ED6A296E225BB63" modified="2025-10-08" version="2">MAQASSSGLPGKSETRRRTSPNSRRKPSISKVILGFVADKGAGNRVSLAALKKAVATRGYNMTRNAWRFKRVLQGLVDKGMLKQVTGKGATGSFLMGKNHASKFKLKAKRRRQRRQRQRGQRRPVQRQLLVGSKPGHKRPIKGVRRAAKCRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HM02</accession>
    <name>A0A4W2HM02_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear envelope integral membrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NEMP1</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398094.1">
        <property type="nucleotide sequence ID" value="XM_027542293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HM02"/>
    <dbReference type="STRING" id="30522.A0A4W2HM02"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026396.1">
        <property type="protein sequence ID" value="ENSBIXP00000036246.1"/>
        <property type="gene ID" value="ENSBIXG00000003529.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001944.1">
        <property type="protein sequence ID" value="ENSBIXP00005032365.1"/>
        <property type="gene ID" value="ENSBIXG00005012888.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892858"/>
    <dbReference type="CTD" id="23306"/>
    <dbReference type="GeneTree" id="ENSGT00390000002174"/>
    <dbReference type="OMA" id="MAGCMKM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043131">
        <property type="term" value="P:erythrocyte enucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598:SF2">
        <property type="entry name" value="NUCLEAR ENVELOPE INTEGRAL MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="50958" checksum="7DD73BB3322BA8A4" modified="2019-09-18" version="1">MAGGMKVAVLPAVGAGPWSWGAGGCGAVRLLLVLFGCFVCGSAGIDLNVVTLRESEILFMNTSRQSCYKNVLIPKWHDIWTRIQIRVNSSKLVRVTQVENEDKLKELEQFSIWNFFSSFLKEKLNDTYINVGLYSTKTCLKVEILEEDTKYSVIVTRRFDPKLFLIFLLGLTLFFCGDLLSRSQIFYYSTGMSVGIVASLLIIIFIVSKFMPKKSPIYIILVGGWSFSLYLIQLVFKNLQEIWRCYWQYLLSYVLAVGFMSFAVCYKYGPLENERSINLLTWTLQLLGLCFMYSSIQIPHIALAIVVIALCTKNLDYPIHWLYITYRKMCKATEKTVPPRLLTEEEYRLQGEVETRKALEQLREYCNSPDCSAWKTVSRIQSPKRFADFVEGSFHLTPNEVSVHEQEYGLGSIIAQDELSEETSSEEEDSDSRYPLVVQQNSFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HRA6</accession>
    <name>A0A4W2HRA6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Canopy FGF signaling regulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CNPY2</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the canopy family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398034.1">
        <property type="nucleotide sequence ID" value="XM_027542233.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398035.1">
        <property type="nucleotide sequence ID" value="XM_027542234.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398037.1">
        <property type="nucleotide sequence ID" value="XM_027542236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HRA6"/>
    <dbReference type="SMR" id="A0A4W2HRA6"/>
    <dbReference type="STRING" id="30522.A0A4W2HRA6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026031.1">
        <property type="protein sequence ID" value="ENSBIXP00000014869.1"/>
        <property type="gene ID" value="ENSBIXG00000003477.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017769.1">
        <property type="protein sequence ID" value="ENSBIXP00005033704.1"/>
        <property type="gene ID" value="ENSBIXG00005005638.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892836"/>
    <dbReference type="CTD" id="10330"/>
    <dbReference type="GeneTree" id="ENSGT00940000161158"/>
    <dbReference type="OMA" id="HLKCLVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010988">
        <property type="term" value="P:regulation of low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042415">
        <property type="entry name" value="CNPY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13341">
        <property type="entry name" value="MIR-INTERACTING SAPOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13341:SF6">
        <property type="entry name" value="PROTEIN CANOPY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611960" evidence="3">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20668" checksum="DD70D7B3900C3643" modified="2019-09-18" version="1">MKGWGWLALLLGALLGTTWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEVCDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HU34</accession>
    <name>A0A4W2HU34_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PTPN22</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2HU34"/>
    <dbReference type="STRING" id="30522.A0A4W2HU34"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034779.1">
        <property type="protein sequence ID" value="ENSBIXP00000039882.1"/>
        <property type="gene ID" value="ENSBIXG00000023911.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024099.1">
        <property type="protein sequence ID" value="ENSBIXP00005036469.1"/>
        <property type="gene ID" value="ENSBIXG00005018325.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160958"/>
    <dbReference type="OMA" id="MMNQQSK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071225">
        <property type="term" value="P:cellular response to muramyl dipeptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032715">
        <property type="term" value="P:negative regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032717">
        <property type="term" value="P:negative regulation of interleukin-8 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070433">
        <property type="term" value="P:negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903753">
        <property type="term" value="P:negative regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032720">
        <property type="term" value="P:negative regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035644">
        <property type="term" value="P:phosphoanandamide dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000566">
        <property type="term" value="P:positive regulation of CD8-positive, alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002230">
        <property type="term" value="P:positive regulation of defense response to virus by host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071663">
        <property type="term" value="P:positive regulation of granzyme B production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032727">
        <property type="term" value="P:positive regulation of interferon-alpha production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032728">
        <property type="term" value="P:positive regulation of interferon-beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900227">
        <property type="term" value="P:positive regulation of NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042307">
        <property type="term" value="P:positive regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902523">
        <property type="term" value="P:positive regulation of protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034141">
        <property type="term" value="P:positive regulation of toll-like receptor 3 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034145">
        <property type="term" value="P:positive regulation of toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034157">
        <property type="term" value="P:positive regulation of toll-like receptor 7 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034165">
        <property type="term" value="P:positive regulation of toll-like receptor 9 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002685">
        <property type="term" value="P:regulation of leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030217">
        <property type="term" value="P:T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14602">
        <property type="entry name" value="PTPc-N22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047170">
        <property type="entry name" value="PTN12/18/22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047253">
        <property type="entry name" value="PTN22_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983">
        <property type="entry name" value="TYROSINE PHOSPHATSE N18, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983:SF1">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="203"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="656"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="717"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="762"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="787"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="543"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005036469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="808" mass="90799" checksum="A9D69D00D9BC0B34" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYT2</accession>
    <name>A0A4W2HYT2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NEDD4 binding protein 2 like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">N4BP2L1</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027413261.1">
        <property type="nucleotide sequence ID" value="XM_027557460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HYT2"/>
    <dbReference type="SMR" id="A0A4W2HYT2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014840.1">
        <property type="protein sequence ID" value="ENSBIXP00000026996.1"/>
        <property type="gene ID" value="ENSBIXG00000028994.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021195.1">
        <property type="protein sequence ID" value="ENSBIXP00005035223.1"/>
        <property type="gene ID" value="ENSBIXG00005016371.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902328"/>
    <dbReference type="CTD" id="90634"/>
    <dbReference type="GeneTree" id="ENSGT00940000161564"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026302">
        <property type="entry name" value="NEDD4-bd_p2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13308">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="173" mass="20472" checksum="521B8AC06D976205" modified="2019-09-18" version="1">MEESFLESFGRLSLRQQQPPPPRPPAPPPLRGTPPRRHSFRKHLYLLRGLPGSGKTTLARQLQHDFPRALIFSTDDFFFREDGAYEFNPDFLEEAHEWNQKRARKAMRNGISPIIIDNTNLHAWEMKPYAVMVFQTEQKNLFRLEMDMVVFRPEMKKHSWSPKRENTPNERTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I0L8</accession>
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        <submittedName>
            <fullName evidence="7">Formin like 2</fullName>
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    </protein>
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        <name evidence="7" type="primary">FMNL2</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
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    <dbReference type="RefSeq" id="XP_027416349.1">
        <property type="nucleotide sequence ID" value="XM_027560548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I0L8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039770.1">
        <property type="protein sequence ID" value="ENSBIXP00000042135.1"/>
        <property type="gene ID" value="ENSBIXG00000026245.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026306.1">
        <property type="protein sequence ID" value="ENSBIXP00005037424.1"/>
        <property type="gene ID" value="ENSBIXG00005019262.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903883"/>
    <dbReference type="CTD" id="114793"/>
    <dbReference type="GeneTree" id="ENSGT00940000155515"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000001">
        <property type="entry name" value="Formin-like 1, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000045">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formin, FH2 domain"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="FH2_Formin"/>
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        <property type="entry name" value="FH2_Formin_sf"/>
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        <property type="entry name" value="FH3_dom"/>
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    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
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        <property type="entry name" value="GBD/FH3_dom"/>
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        <property type="entry name" value="GTPase-bd"/>
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        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORMIN-LIKE PROTEIN 2"/>
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    </dbReference>
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        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
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        <property type="entry name" value="Drf_FH3"/>
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    </dbReference>
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        <property type="entry name" value="Drf_GBD"/>
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    </dbReference>
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        <property type="entry name" value="FH2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="23"/>
            <end position="469"/>
        </location>
    </feature>
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        <location>
            <begin position="617"/>
            <end position="1008"/>
        </location>
    </feature>
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        <location>
            <begin position="1040"/>
            <end position="1077"/>
        </location>
    </feature>
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        <location>
            <begin position="521"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="381"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="548"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="583"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123473" checksum="35084A60479954E0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IFZ2</accession>
    <name>A0A4W2IFZ2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small integral membrane protein 36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SMIM36</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSBIXP00000028621.1"/>
        <property type="gene ID" value="ENSBIXG00000000480.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005044243.1"/>
        <property type="gene ID" value="ENSBIXG00005028057.1"/>
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        <property type="component" value="Chromosome 19"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="93" mass="10291" checksum="3C14316D52FE09BE" modified="2019-09-18" version="1">MEFYLEIDPVTLNLIILVSSYVILLLVFLISCVLYDCRGKDPSKEYAPEASLDAQPSIRLVVMQQGSPGAPWARRSSLHFGNHTPVGKKSTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BKB3</accession>
    <name>A0A4W2BKB3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ARPC1B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).</text>
    </comment>
    <comment type="function">
        <text evidence="11">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027382241.1">
        <property type="nucleotide sequence ID" value="XM_027526440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BKB3"/>
    <dbReference type="SMR" id="A0A4W2BKB3"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000053491.1">
        <property type="protein sequence ID" value="ENSBIXP00000000255.1"/>
        <property type="gene ID" value="ENSBIXG00000030044.1"/>
    </dbReference>
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    <dbReference type="CTD" id="10095"/>
    <dbReference type="OMA" id="IWDVKVT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000030">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10709:SF10">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="40976" checksum="B412F9C50DC93748" modified="2019-09-18" version="1">MAYHSFLVEPISCHAWNKDRTQIAICPNNHEVHIYEKSGNKWVQVHELKEHNGQVTGIDWAPDSNRIVTCGTDRNAYVWTLKGRTWKPTLVILRINRAARCVRWAPNEKKFAVGSGSRVISICYFEQENDWWVCKHIKKPIRSTVLSLDWHPNSVLLAAGSCDFKCRIFSAYIKEVEERPAPTPWGSKMPFGELMFESSSSCGWVHGVCFSANGSRVAWVSHDSTVCLADADKKMAVATLASETLPLLAVTFITESSLVAAGHDCFPVLFTYDSAAGKLSFGGRLDVPKQSSQRGLTARERFQNLDKKASSEGSAAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGMDGGMSIWDVRSLESALKDLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRL7</accession>
    <name>A0A4W2BRL7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ral transcription factor IIIC subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GTF3C1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2BRL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011515.1">
        <property type="protein sequence ID" value="ENSBIXP00000002684.1"/>
        <property type="gene ID" value="ENSBIXG00000000230.1"/>
    </dbReference>
    <dbReference type="OMA" id="TIIQDGI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16169">
        <property type="entry name" value="Tau138_eWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056467">
        <property type="entry name" value="eWH_GTF3C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035625">
        <property type="entry name" value="Tfc3-like_eWH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056428">
        <property type="entry name" value="WH_GTF3C1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24101">
        <property type="entry name" value="WHD_GTF3C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23704">
        <property type="entry name" value="WHD_GTF3C1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="General transcription factor 3C polypeptide 1 winged-helix" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="174"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="GTF3C1 extended winged-helix" evidence="9">
        <location>
            <begin position="610"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="470"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="715"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1041"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1184"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1591"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1806"/>
            <end position="1891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1905"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1054"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1192"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1217"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1595"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1806"/>
            <end position="1820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2096" mass="236173" checksum="E9496EEE1AF780AC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUR8</accession>
    <name>A0A4W2BUR8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pro-thyrotropin-releasing hormone</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUR8"/>
    <dbReference type="STRING" id="30522.A0A4W2BUR8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010748.1">
        <property type="protein sequence ID" value="ENSBIXP00000003014.1"/>
        <property type="gene ID" value="ENSBIXG00000009430.1"/>
    </dbReference>
    <dbReference type="OMA" id="PDWFSKR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008437">
        <property type="term" value="F:thyrotropin-releasing hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042755">
        <property type="term" value="P:eating behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001692">
        <property type="term" value="P:histamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014050">
        <property type="term" value="P:negative regulation of glutamate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014054">
        <property type="term" value="P:positive regulation of gamma-aminobutyric acid secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032024">
        <property type="term" value="P:positive regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008857">
        <property type="entry name" value="TRH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17530">
        <property type="entry name" value="PRO-THYROTROPIN-RELEASING HORMONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17530:SF2">
        <property type="entry name" value="PRO-THYROTROPIN-RELEASING HORMONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05438">
        <property type="entry name" value="TRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001795">
        <property type="entry name" value="TRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5 9" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021523966" description="Pro-thyrotropin-releasing hormone" evidence="9">
        <location>
            <begin position="25"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="241" mass="26808" checksum="5C5129E8DFAFF021" modified="2019-09-18" version="1">MPGSWFLLAMALTLTLTGVPGGRAQLDVAQQEADLSEQQGLEDLLRQAERLLLLREDLQRLREDQDDSVSESQIFQPDWFSKRQHPGKREEEEADEAVEGEEEGGAAGLHKRQHPGRRGDVAAWAVDAGQQKRQHPGRRASWLGYAVTKRQHPGRRLVDPRAQRSWEEAGEDEDEGGELMSEKRQHPGKRALGSPCGPRGPCGQASLLLGLLDDLSRGQGAEEKRQHPGRRAAWASEPLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BV33</accession>
    <name>A0A4W2BV33_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase RNFT1</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING finger and transmembrane domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BV33"/>
    <dbReference type="STRING" id="30522.A0A4W2BV33"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011033.1">
        <property type="protein sequence ID" value="ENSBIXP00000002863.1"/>
        <property type="gene ID" value="ENSBIXG00000009310.1"/>
    </dbReference>
    <dbReference type="OMA" id="GCIHSRL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16741">
        <property type="entry name" value="RING-HC_RNFT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000440">
        <property type="entry name" value="RING finger and transmembrane domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044235">
        <property type="entry name" value="RNFT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNFT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860">
        <property type="entry name" value="UNCHARACTERIZED RING FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="241"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="373"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="433" mass="49613" checksum="A6E42271A1793660" modified="2019-09-18" version="1">FSLFLPSLPGPRSAHRHERRQSREAKKSQNKYSRAMQANCSQLHSPSGAAGSEDASASQCVQARLTGEASCLYSGDVHIPISSLPKECAENPSSRNTRSGVHSCTHGCTHSRLRSHTHNEARQPDDSDMEYGDHGSSSFSEFRYLFKWLQKSLPYILILGVKLVMQHTTGISLGIGLLTTFIYANKSIVNQVFLRERCSKIQCAWLLVFLAGSSVLLYYTFHSQSLYYSLIFLNPTLDYSSFWEVLWVVGITDFILKFLFMGLKCLILLVPSLIMPFKSKGYWYMLLEELCQYYRTFVPIPVWFRYLISYGEFGNVTRWSLGILLALLYLILKLLDFFGHLRTFRQVLRIFVTRPSYGVPASKRQCSDVDDICSICQAEFQKPILLICQHIFCEECITLWFNREKTCPLCRTVISDHINKWKDGATSSHLQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWG1</accession>
    <name>A0A4W2BWG1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Out at first protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OAF</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419273.1">
        <property type="nucleotide sequence ID" value="XM_027563472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BWG1"/>
    <dbReference type="STRING" id="30522.A0A4W2BWG1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009961.1">
        <property type="protein sequence ID" value="ENSBIXP00000003384.1"/>
        <property type="gene ID" value="ENSBIXG00000009790.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042677.1">
        <property type="protein sequence ID" value="ENSBIXP00005026440.1"/>
        <property type="gene ID" value="ENSBIXG00005028761.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905747"/>
    <dbReference type="CTD" id="220323"/>
    <dbReference type="GeneTree" id="ENSGT00390000012008"/>
    <dbReference type="OMA" id="CHYGLSL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026315">
        <property type="entry name" value="Oaf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053897">
        <property type="entry name" value="Oaf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053894">
        <property type="entry name" value="OAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13423">
        <property type="entry name" value="OUT AT FIRST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13423:SF2">
        <property type="entry name" value="OUT AT FIRST PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22873">
        <property type="entry name" value="OAF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14941">
        <property type="entry name" value="OAF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610299" description="Out at first protein homolog" evidence="3">
        <location>
            <begin position="34"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Out at first protein BRICHOS-like" evidence="4">
        <location>
            <begin position="42"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Out at first C-terminal" evidence="5">
        <location>
            <begin position="215"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="282" mass="31419" checksum="AB62182A635EBA83" modified="2019-09-18" version="1">MRPPGRCGVPRASPALLLLTLLVPLLWAPRGVGAGAGAGAPSELRVRVRLPDGQVTEESLQADSDADSISLELRKPDGTLISFTADFKKDVKIFRALILGELEKGQSQFQALCFVTRLHHNEIIPSEAMAKLRQKNPRAVRQAEEVRGPEQMHMDIAVNFSQGALLSPHLRNVCAEAVDAIYTRQEDVRFWLEQGVDGSVFEALPKASEQVLLPRCGQVGDRGKPCICRYGLSLAWYPCMLKYCHGRDRPAPYKCGIRSCQKNYSFDFYVPQKQLCLWDEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BY75</accession>
    <name>A0A4W2BY75_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD repeat-containing protein 46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">WDR46</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Scaffold component of the nucleolar structure. Required for localization of DDX21 and NCL to the granular compartment of the nucleolus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with DDX21, NCL, NOP2 and EBNA1BP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BY75"/>
    <dbReference type="SMR" id="A0A4W2BY75"/>
    <dbReference type="STRING" id="30522.A0A4W2BY75"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005993.1">
        <property type="protein sequence ID" value="ENSBIXP00000005320.1"/>
        <property type="gene ID" value="ENSBIXG00000011538.1"/>
    </dbReference>
    <dbReference type="OMA" id="EFLPYHW"/>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="WD repeat domain 46"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
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        <location>
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            <end position="513"/>
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        <location>
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            <end position="66"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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            <end position="541"/>
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    </feature>
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        <location>
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            <end position="578"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="605" mass="67524" checksum="02D37F45393B41F7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYZ6</accession>
    <name>A0A4W2BYZ6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2BYZ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007865.1">
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        <property type="gene ID" value="ENSBIXG00000010758.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Gprotein_alpha_I"/>
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    </dbReference>
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        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF67">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-alpha"/>
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    </dbReference>
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        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
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        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="9" id="KW-0844">Vision</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="170"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="195"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="264"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="349" mass="39895" checksum="60B86F2A79C158DB" modified="2019-09-18" version="1">MGAGSAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZB6</accession>
    <name>A0A4W2BZB6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mesencephalic astrocyte derived neurotrophic factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MANF</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARMET family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZB6"/>
    <dbReference type="STRING" id="30522.A0A4W2BZB6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007623.1">
        <property type="protein sequence ID" value="ENSBIXP00000004542.1"/>
        <property type="gene ID" value="ENSBIXG00000010862.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009501.1">
        <property type="protein sequence ID" value="ENSBIXP00005029904.1"/>
        <property type="gene ID" value="ENSBIXG00005007421.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007160"/>
    <dbReference type="OMA" id="EVCKGCA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071542">
        <property type="term" value="P:dopaminergic neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045333">
        <property type="entry name" value="ARMET-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019345">
        <property type="entry name" value="ARMET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045332">
        <property type="entry name" value="ARMET_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12990">
        <property type="entry name" value="ARMET-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12990:SF10">
        <property type="entry name" value="MESENCEPHALIC ASTROCYTE-DERIVED NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10208">
        <property type="entry name" value="ARMET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20145">
        <property type="entry name" value="ARMET_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610314" evidence="6">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="ARMET N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="ARMET C-terminal" evidence="7">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20830" checksum="B8A366957138AF15" modified="2025-10-08" version="2">MWRMWATHGLAVALALSVLPASRALRQGDCEVCISYLGRFYQDLKDRDVTFSPASIEKELIKFCREARGKENRLCYYIGATEDAATKIINEVSKPLSHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASSRTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZV4</accession>
    <name>A0A4W2BZV4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head protein 9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RSPH9</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the flagellar radial spoke RSP9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379768.1">
        <property type="nucleotide sequence ID" value="XM_027523967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZV4"/>
    <dbReference type="SMR" id="A0A4W2BZV4"/>
    <dbReference type="STRING" id="30522.A0A4W2BZV4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007177.1">
        <property type="protein sequence ID" value="ENSBIXP00000004746.1"/>
        <property type="gene ID" value="ENSBIXG00000011057.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014495.1">
        <property type="protein sequence ID" value="ENSBIXP00005001300.1"/>
        <property type="gene ID" value="ENSBIXG00005029313.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881159"/>
    <dbReference type="CTD" id="221421"/>
    <dbReference type="GeneTree" id="ENSGT00390000018686"/>
    <dbReference type="OMA" id="TFYHVPN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060091">
        <property type="term" value="C:kinocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120336">
        <property type="term" value="C:radial spoke head 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120338">
        <property type="term" value="C:radial spoke head 3"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904158">
        <property type="term" value="P:axonemal central apparatus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062177">
        <property type="term" value="P:radial spoke assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055316">
        <property type="entry name" value="RSP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="276" mass="31294" checksum="BACEE065190F3DDB" modified="2019-09-18" version="1">MDAESLLLALELASGSGQGLSPDRRASLLTSLMLVKRDYRFDRVLFWGRILGLVADYYIAQGVSEDQLAPRKTLYSLNCMEWSLLPPATKEMETQTSVVKGRFMGDPSHEYEHTELQKVNEGEKVFEEEVVVQIKEETRLVSVIDQIDKAVAVIPRGALFKTPFGPINVNRTFEGLSLSEAKKLSSYFHFREPVELKNKTLLEKAELDPSLDFMDSLEHDIPKGSWSVQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYIYVGTGEKNLDLPFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1I5</accession>
    <name>A0A4W2C1I5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C1I5"/>
    <dbReference type="STRING" id="30522.A0A4W2C1I5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053757.1">
        <property type="protein sequence ID" value="ENSBIXP00000000130.1"/>
        <property type="gene ID" value="ENSBIXG00000030226.1"/>
    </dbReference>
    <dbReference type="OMA" id="QMDWEKV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051762">
        <property type="entry name" value="UBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46318">
        <property type="entry name" value="UPSTREAM BINDING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46318:SF1">
        <property type="entry name" value="UPSTREAM-BINDING FACTOR 1-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="115"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="115"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="171" mass="20380" checksum="1C6FC1FA416575EC" modified="2019-09-18" version="1">MTELPGPYNCPKDLEMVLPKSQDDWSKEDIVHLLEYMEKSIPSNDRHTFKMTQSMVDWEKVAFKDFSGEKCKLKWLEISYNLRKFHTFKEVIQEAKENVNNPSKSRNHKKHPDLPKRPLTAYLLFFKEMRPQYLQKHPKMSNQELTKVLSEEYRKLPEHLKTQAVSWGGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3T4</accession>
    <name>A0A4W2C3T4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">U6 snRNA-associated Sm-like protein LSm2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LSM2</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3T4"/>
    <dbReference type="SMR" id="A0A4W2C3T4"/>
    <dbReference type="STRING" id="30522.A0A4W2C3T4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003796.1">
        <property type="protein sequence ID" value="ENSBIXP00000006418.1"/>
        <property type="gene ID" value="ENSBIXG00000012534.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024900.1">
        <property type="protein sequence ID" value="ENSBIXP00005036805.1"/>
        <property type="gene ID" value="ENSBIXG00005018478.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016597"/>
    <dbReference type="OMA" id="DNISCTD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120115">
        <property type="term" value="C:Lsm2-8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000009">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005036805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="95" mass="10835" checksum="623591A09A6ABACE" modified="2019-09-18" version="1">MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVTDPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQDAARKEALQQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C520</accession>
    <name>A0A4W2C520_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA splicing endonuclease subunit 54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TSEN54</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C520"/>
    <dbReference type="STRING" id="30522.A0A4W2C520"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002893.1">
        <property type="protein sequence ID" value="ENSBIXP00000006892.1"/>
        <property type="gene ID" value="ENSBIXG00000006385.1"/>
    </dbReference>
    <dbReference type="OMA" id="AMVLQHI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.150:FF:000001">
        <property type="entry name" value="tRNA splicing endonuclease subunit 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024337">
        <property type="entry name" value="tRNA_splic_suSen54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024336">
        <property type="entry name" value="tRNA_splic_suSen54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027">
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR21027:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
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        <property type="entry name" value="tRNA_int_end_N2"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
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        <location>
            <begin position="63"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="27"/>
            <end position="46"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="191"/>
            <end position="295"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="528" mass="59367" checksum="02C5222C85899B2A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C530</accession>
    <name>A0A4W2C530_BOBOX</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        </source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7C1</accession>
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        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7C1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001273.1">
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        <property type="gene ID" value="ENSBIXG00000013637.1"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
    </feature>
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            <end position="29"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="811" mass="91254" checksum="ADB3AF1D8B55E79D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C997</accession>
    <name>A0A4W2C997_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">Zinc finger protein 710</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZNF710</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377917.1">
        <property type="nucleotide sequence ID" value="XM_027522116.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A4W2C997"/>
    <dbReference type="SMR" id="A0A4W2C997"/>
    <dbReference type="STRING" id="30522.A0A4W2C997"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016115.1">
        <property type="protein sequence ID" value="ENSBIXP00000008428.1"/>
        <property type="gene ID" value="ENSBIXG00000014274.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047972.1">
        <property type="protein sequence ID" value="ENSBIXP00005016001.1"/>
        <property type="gene ID" value="ENSBIXG00005019736.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880101"/>
    <dbReference type="CTD" id="374655"/>
    <dbReference type="GeneTree" id="ENSGT00940000161170"/>
    <dbReference type="OMA" id="KEMAYYS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000161">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000182">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000186">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000191">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000203">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001180">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000451">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000502">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000649">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000684">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390:SF79">
        <property type="entry name" value="ASPARAGINE-RICH ZINC FINGER PROTEIN AZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="575"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="664" mass="74527" checksum="A4936F25F5AE3431" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAN9</accession>
    <name>A0A4W2CAN9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Torsin 1A interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113906816</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TOR1AIP family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000008974.1"/>
        <property type="gene ID" value="ENSBIXG00000014682.1"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="torsin-1A-interacting protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038599">
        <property type="entry name" value="LAP1C-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008662">
        <property type="entry name" value="TOIP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046753">
        <property type="entry name" value="TOIP1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046754">
        <property type="entry name" value="TOIP1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18843">
        <property type="entry name" value="TORSIN-1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18843:SF2">
        <property type="entry name" value="TORSIN-1A-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05609">
        <property type="entry name" value="LAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20443">
        <property type="entry name" value="LAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Torsin-1A-interacting protein 1/2 N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Torsin-1A-interacting protein 1/2 AAA+ activator" evidence="12">
        <location>
            <begin position="236"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="51604" checksum="B10043D2A0699AE2" modified="2019-09-18" version="1">MTDRGLRDPPEDPQKDLENDPSLNTQEATVTTSTTEEAQTPDPAPGLNKDHQEIETVGPGSTDTDAQSENPDETDCGKYLKDTEGENSQSFQGGEQGHQLPSESLSEDPVDPETKCSPSDKVGRTDAHIVGSSAALPEHVSDGPGASQELPVVDPQDAQSPGHSSAGLESQHTLRRRLLAPETETHQQETQELEENKEKNAQGTIRRIDKKDFLSYGSIIFCFLVLIIVLLSSVNSYYSSPAQQVPKNPALEAFLVHFSQLKDKFPGQSSFLWQRGRKFLQKHLNASHPSEPATIIFTAAREGRETLKCLSHHVADAYTSAQKVSAIQIDGAGKTLQDSDKVKHSVDRELSSGFEKGQKAAVVHHFESLPAGSTLIFYKYCDHENAAFKDVALVLTVLLEEETLEASVSPRETEEKVRDLLWARFTNSNTPSSFNHMDSDKLSGLWSRISHLVLPVQPVRSIEERGCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CC65</accession>
    <name>A0A4W2CC65_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CC65"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017834.1">
        <property type="protein sequence ID" value="ENSBIXP00000009521.1"/>
        <property type="gene ID" value="ENSBIXG00000006455.1"/>
    </dbReference>
    <dbReference type="OMA" id="SHIRFYR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="107"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="574" mass="66659" checksum="E43152221D1D1F49" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCN0</accession>
    <name>A0A4W2CCN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SLC4A1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Component of the ankyrin-1 complex in the erythrocyte, composed of ANK1, RHCE, RHAG, SLC4A1, EPB42, GYPA, GYPB and AQP1. Interacts with STOM; this interaction positively regulates SLC4A1 activity. Interacts with GYPA; a GYPA monomer is bound at each end of the SLC4A1 dimer forming a heterotetramer. Three SLC4A1 dimers (Band 3-I, Band 3-II and Band 3-III) participates in the ankyrin-1 complex. Interacts (via the cytoplasmic domain) with EPB42; this interaction is mediated by the SLC4A1 Band 3-I dimer. Interacts (via the cytoplasmic domain) directly with ANK1; this interaction is mediated by the SLC4A1 Band 3-II and Band 3-III dimers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with TMEM139.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCN0"/>
    <dbReference type="STRING" id="30522.A0A4W2CCN0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019558.1">
        <property type="protein sequence ID" value="ENSBIXP00000010635.1"/>
        <property type="gene ID" value="ENSBIXG00000016085.1"/>
    </dbReference>
    <dbReference type="OMA" id="YSHFPIW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015106">
        <property type="term" value="F:bicarbonate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000015">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001717">
        <property type="entry name" value="Anion_exchange"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002977">
        <property type="entry name" value="Anion_exchange_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018241">
        <property type="entry name" value="Anion_exchange_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF12">
        <property type="entry name" value="BAND 3 ANION TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00165">
        <property type="entry name" value="ANIONEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01187">
        <property type="entry name" value="ANIONEXHNGR1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00219">
        <property type="entry name" value="ANION_EXCHANGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00220">
        <property type="entry name" value="ANION_EXCHANGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="587"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="638"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="670"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="712"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="751"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="806"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="978"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1004"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="22">
        <location>
            <begin position="194"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="21">
        <location>
            <begin position="524"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="21">
        <location>
            <begin position="710"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1059" mass="118113" checksum="931852C470E95B80" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCN6</accession>
    <name>A0A4W2CCN6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transposase 22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCN6"/>
    <dbReference type="STRING" id="30522.A0A4W2CCN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019550.1">
        <property type="protein sequence ID" value="ENSBIXP00000010640.1"/>
        <property type="gene ID" value="ENSBIXG00000016084.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTECKWI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1820:FF:000002">
        <property type="entry name" value="LINE-1 retrotransposable element ORF1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.390">
        <property type="entry name" value="L1 transposable element, trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042566">
        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035300">
        <property type="entry name" value="L1_dsRBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043636">
        <property type="entry name" value="L1_RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035301">
        <property type="entry name" value="L1_trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17490">
        <property type="entry name" value="Tnp_22_dsRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17489">
        <property type="entry name" value="Tnp_22_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02994">
        <property type="entry name" value="Transposase_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L1 transposable element trimerization" evidence="4">
        <location>
            <begin position="40"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="L1 transposable element RRM" evidence="3">
        <location>
            <begin position="81"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="L1 transposable element dsRBD-like" evidence="5">
        <location>
            <begin position="181"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="29376" checksum="0FBF3D3A2BDD2864" modified="2019-09-18" version="1">MQERFNKDLEEIKESQYIMNNAINEIKNTLEATNSRITEAEDRISELEDRMVEISESERIKEKRIKRNEDNLRDLQDNIKRYNIRIIGVPEEEDKKKDHEKILEEIIVENFPKMGKEIITQVQETQRVPNRINPRQNTPRHILIKLTKIKHKEQILKAAREKQQITHKGIPIRITADLSIETLQARREWQDILKVMKENNLQPRLLYLARISFKYEGEIKSFSDKQKLREFSTTKPALQQILKDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCT4</accession>
    <name>A0A4W2CCT4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CKLF like MARVEL transmembrane domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCT4"/>
    <dbReference type="STRING" id="30522.A0A4W2CCT4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018204.1">
        <property type="protein sequence ID" value="ENSBIXP00000009746.1"/>
        <property type="gene ID" value="ENSBIXG00000015378.1"/>
    </dbReference>
    <dbReference type="OMA" id="PPKFRDS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF43">
        <property type="entry name" value="CKLF-LIKE MARVEL TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="29590" checksum="A1F10BF150BF614F" modified="2019-09-18" version="1">MVPRAHSPRLAGKTEVAALRGSRVPRRHILVATRVPGGLGLSAGSHSCVRPRGRGGAQPLGRDAGSQGRAGRRRARFRATGPVGHHQAQGSTMDAEKENPPSRASKSGRPPARSVRIAEGPHPERSSGSTLSEHSVSAAPSAYILKQPSTPATLSQEEKEAIQKRAEGRAKVPPKFRDSIKRFFFSPTGALKIIRLGLLIGAFIFFIIAEAPESYIAITILETCIVVFFILIYMLTLHHLMTYLHWPLLVSVPFHNLHINFALSPRVKAVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEK2</accession>
    <name>A0A4W2CEK2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEK2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020750.1">
        <property type="protein sequence ID" value="ENSBIXP00000011404.1"/>
        <property type="gene ID" value="ENSBIXG00000002599.1"/>
    </dbReference>
    <dbReference type="OMA" id="NYSTITF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="32"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="362" mass="41257" checksum="5071822765380425" modified="2019-09-18" version="1">MNKASGGDGIAVDIFQILKDDTVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYSTITFISHASKVMLKILQARLQQHINRELPDVQADFRKGRGTRDQIANIYWIIEKAREFQKSIYFCFIDYAFDCVDHNKLWKILKEMGVPDHLSCLLRNLYAGQEATVRTGHGTTDWFQIGNGVRQGCILSPCLFNFYAEYIMRNTGLEESQAGVKIAGRNINNLRYTDDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWQIDGETVETVADFIFLGTKITADGDCSHEIKRCLLLGRKVITNLDNILKSRDITLPTKVRLVKAMVFPVVIYGCESWTIKKAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFA7</accession>
    <name>A0A4W2CFA7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Coiled-coil domain containing 88A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CCDC88A</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021438184" description="UPAR/Ly6 domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="7">
        <location>
            <begin position="139"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="253" mass="27314" checksum="FDAC6D8ED8FDC81C" modified="2019-09-18" version="1">MGACHFQGLLLLFLVGAPTLIMAQKLFCQKGTFMGIQEDATNMFNWTSEKVEACDNGTLCQETILLIKTAGTKTAILATKSCSLDGTPAITFIQHTAAPSLAAISYSNYCEDPFCNNREGLYDIWNIQETEEETKGTTSLHCPTCLALGSCLNAPSVACPNNTDRCYQGKLQVSEGNVNSLLEIKGCTSIIGCRLMSGVFKIGPLWVKETCPSMSISTRKIDNGATWLHTSVWKLKLLLMLLLLILGGSASGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIX9</accession>
    <name>A0A4W2CIX9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIX9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021450.1">
        <property type="protein sequence ID" value="ENSBIXP00000011873.1"/>
        <property type="gene ID" value="ENSBIXG00000017058.1"/>
    </dbReference>
    <dbReference type="OMA" id="MNASWER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="418" mass="48149" checksum="83B9A486A6809FB3" modified="2019-09-18" version="1">MKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKSIYFCFIDYAKAFDCVDHNKLWEILKEMGTPDHLICLLRNLHAGQEATVRTGHGTTDWFQIGNRVHQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITTDGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAECRRIDAFAEWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJB4</accession>
    <name>A0A4W2CJB4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Caveolin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CAV2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Caveola</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395391.1">
        <property type="nucleotide sequence ID" value="XM_027539590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJB4"/>
    <dbReference type="STRING" id="30522.A0A4W2CJB4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023497.1">
        <property type="protein sequence ID" value="ENSBIXP00000013194.1"/>
        <property type="gene ID" value="ENSBIXG00000018164.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009237.1">
        <property type="protein sequence ID" value="ENSBIXP00005003990.1"/>
        <property type="gene ID" value="ENSBIXG00005009987.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891495"/>
    <dbReference type="CTD" id="858"/>
    <dbReference type="GeneTree" id="ENSGT00950000183006"/>
    <dbReference type="OMA" id="TRIFMDD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002080">
        <property type="term" value="C:acrosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002095">
        <property type="term" value="C:caveolar macromolecular signaling complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032795">
        <property type="term" value="F:heterotrimeric G-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097110">
        <property type="term" value="F:scaffold protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071711">
        <property type="term" value="P:basement membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044794">
        <property type="term" value="P:host-mediated activation of viral process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001937">
        <property type="term" value="P:negative regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014859">
        <property type="term" value="P:negative regulation of skeletal muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060161">
        <property type="term" value="P:positive regulation of dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019065">
        <property type="term" value="P:receptor-mediated endocytosis of virus by host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014856">
        <property type="term" value="P:skeletal muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019076">
        <property type="term" value="P:viral release from host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018361">
        <property type="entry name" value="Caveolin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF3">
        <property type="entry name" value="CAVEOLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01210">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18160" checksum="863DAA5EADF49AF5" modified="2019-09-18" version="1">MGLETEKADVQLFMDDDSYSRHSSVDYADPDKFVDPGSDRDPHRLNSHLKVGFEDVIAEPVSTHSFDKVWICSHALFEMSKYVIYKFLTVFLAIPLAFAAGILFATLSCLHIWIIMPFVKTCLMVLPSVQTIWKSVTDVVIAPLCTSIGRSFSSVSLQLSHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLG7</accession>
    <name>A0A4W2CLG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PET100 cytochrome c oxidase chaperone</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLG7"/>
    <dbReference type="STRING" id="30522.A0A4W2CLG7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046284.1">
        <property type="protein sequence ID" value="ENSBIXP00000013951.1"/>
        <property type="gene ID" value="ENSBIXG00000003273.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021013.1">
        <property type="protein sequence ID" value="ENSBIXP00005035143.1"/>
        <property type="gene ID" value="ENSBIXG00005007509.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016884"/>
    <dbReference type="OMA" id="WRLEVFR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="10108" checksum="455836E2B6A052F7" modified="2019-09-18" version="1">MGVKLEVFRMTIYLTFPVAMFWIANQAEWFEDYVIQRKRELWPPEKEDQRRELEEFKERRRKQRQEKLPRPSSQSNSHIHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ47</accession>
    <name>A0A4W2CQ47_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQ47"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016122.1">
        <property type="protein sequence ID" value="ENSBIXP00000008444.1"/>
        <property type="gene ID" value="ENSBIXG00000014287.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900118">
        <property type="term" value="P:negative regulation of execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20245">
        <property type="entry name" value="humanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028139">
        <property type="entry name" value="Humanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33895">
        <property type="entry name" value="HUMANIN-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33895:SF5">
        <property type="entry name" value="HUMANIN-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15040">
        <property type="entry name" value="Humanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="24" mass="2781" checksum="0E761A18F778D4B1" modified="2019-09-18" version="1">MATRGFYCLLLPTSEIDLPVKRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ88</accession>
    <name>A0A4W2CQ88_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="9">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EIF5A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027374000.1">
        <property type="nucleotide sequence ID" value="XM_027518199.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027374001.1">
        <property type="nucleotide sequence ID" value="XM_027518200.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027374002.1">
        <property type="nucleotide sequence ID" value="XM_027518201.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027374005.1">
        <property type="nucleotide sequence ID" value="XM_027518204.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027374006.1">
        <property type="nucleotide sequence ID" value="XM_027518205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQ88"/>
    <dbReference type="SMR" id="A0A4W2CQ88"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016183.1">
        <property type="protein sequence ID" value="ENSBIXP00000008486.1"/>
        <property type="gene ID" value="ENSBIXG00000000964.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877757"/>
    <dbReference type="CTD" id="1984"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
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        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
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        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
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        <property type="entry name" value="eIF-5a"/>
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    <dbReference type="Pfam" id="PF21485">
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        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Translation proteins SH3-like domain"/>
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            <begin position="83"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="154" mass="16832" checksum="07EF043C7DEA3091" modified="2019-09-18" version="1">MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQF6</accession>
    <name>A0A4W2CQF6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4">BPTI/Kunitz inhibitor domain-containing protein</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSBIXP00000014192.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005011851.1"/>
        <property type="gene ID" value="ENSBIXG00005016194.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Kunitz_BPTI"/>
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        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kunitz_BPTI"/>
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        <property type="entry name" value="Kunitz_BPTI_sf"/>
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        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="4">
        <location>
            <begin position="37"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="95" mass="10744" checksum="6DF6B6345EEC1ED5" modified="2019-09-18" version="1">DAFTLSVCFFCRDLAVTTILLFHRDPPIPIALQPDICLEPPYMGPCKTVMIRYFYNAYTGLCERFRYGGCGGNKNNFLTKEECMKTCGQVAGSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUD8</accession>
    <name>A0A4W2CUD8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUD8"/>
    <dbReference type="STRING" id="30522.A0A4W2CUD8"/>
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        <property type="protein sequence ID" value="ENSBIXP00000016973.1"/>
        <property type="gene ID" value="ENSBIXG00000021045.1"/>
    </dbReference>
    <dbReference type="OMA" id="VEWQTYH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07401">
        <property type="entry name" value="MPP_TMEM62_N"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
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        <property type="entry name" value="Ig_TMM62"/>
    </dbReference>
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        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
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        <property type="entry name" value="MPP_TMEM62"/>
    </dbReference>
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        <property type="entry name" value="TMEM62_C"/>
    </dbReference>
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        <property type="entry name" value="HELICASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14795:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24384">
        <property type="entry name" value="Ig_TMM62"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Metallo-dependent phosphatases"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="526"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="58"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM62 Ig-like" evidence="3">
        <location>
            <begin position="308"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM62 C-terminal" evidence="4">
        <location>
            <begin position="434"/>
            <end position="569"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="642" mass="72287" checksum="99D8AD4530AA5E30" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWZ1</accession>
    <name>A0A4W2CWZ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Activated RNA polymerase II transcriptional coactivator p15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SUB1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SUB1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Interacts with CSTF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375828.1">
        <property type="nucleotide sequence ID" value="XM_027520027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWZ1"/>
    <dbReference type="SMR" id="A0A4W2CWZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019908.1">
        <property type="protein sequence ID" value="ENSBIXP00000010851.1"/>
        <property type="gene ID" value="ENSBIXG00000016280.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878883"/>
    <dbReference type="CTD" id="10923"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.31.10:FF:000001">
        <property type="entry name" value="Activated RNA polymerase II transcriptional coactivator p15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="13">
        <location>
            <begin position="64"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="127" mass="14369" checksum="0DAE0CB1B7EFFE15" modified="2019-09-18" version="1">MPKSKELVSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDAEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXJ3</accession>
    <name>A0A4W2CXJ3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the large ribosomal subunit. May bind IPO9 with low affinity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXJ3"/>
    <dbReference type="STRING" id="30522.A0A4W2CXJ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031001.1">
        <property type="protein sequence ID" value="ENSBIXP00000018139.1"/>
        <property type="gene ID" value="ENSBIXG00000004262.1"/>
    </dbReference>
    <dbReference type="OMA" id="KLWASIT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000020">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049633">
        <property type="entry name" value="Ribosomal_eL6_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005568">
        <property type="entry name" value="Ribosomal_uL6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03868">
        <property type="entry name" value="Ribosomal_L6e_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01170">
        <property type="entry name" value="RIBOSOMAL_L6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 N-terminal" evidence="15">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="346" mass="39729" checksum="93213475E82C6AF2" modified="2019-09-18" version="1">MAGEKAEKPDTKEKKPEAKKADAGKKAKKVEKVKKVKKGKPHCSQNPVLVRGIGRYSRSAMYSRKALYKRKYSAAKSKVEKKKKVRVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSKHVRKLRASITPGTILIILTGRHRGKRVVFLKQLGSGLLLVTGPLSLNRVPLRRTHQKFVIATSTKIDISGVKIPEHLTDTYFKKKKLRKPRHQEGEIFDTEREKYEITEQRKVDQKAVDSQILRRIKAVPQLQGYLRSVFALTNRRANELSEDYRQCSTKVINNHSESTPFLCNENIVKMCPRWLFALFSPINRLLDMMVIRHLSWKKIIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXP6</accession>
    <name>A0A4W2CXP6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 91</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP91 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXP6"/>
    <dbReference type="STRING" id="30522.A0A4W2CXP6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031063.1">
        <property type="protein sequence ID" value="ENSBIXP00000018196.1"/>
        <property type="gene ID" value="ENSBIXG00000022055.1"/>
    </dbReference>
    <dbReference type="OMA" id="VQTMYRD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026720">
        <property type="entry name" value="CFAP91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032840">
        <property type="entry name" value="CFAP91_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455:SF10">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14738">
        <property type="entry name" value="CFAP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP91" evidence="9">
        <location>
            <begin position="191"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="653"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="764" mass="89742" checksum="E2A801BACCDA73BC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CY12</accession>
    <name>A0A4W2CY12_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">KH domain-containing RNA-binding protein QKI</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein quaking</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">QKI</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Homodimer; does not require RNA to homodimerize. Able to heterodimerize with BICC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the quaking family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CY12"/>
    <dbReference type="STRING" id="30522.A0A4W2CY12"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031359.1">
        <property type="protein sequence ID" value="ENSBIXP00000018369.1"/>
        <property type="gene ID" value="ENSBIXG00000022199.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060284">
        <property type="term" value="P:regulation of cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22465">
        <property type="entry name" value="KH-I_Hqk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4010:FF:000001">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000055">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032367">
        <property type="entry name" value="Quaking_NLS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032377">
        <property type="entry name" value="STAR_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF125">
        <property type="entry name" value="KH DOMAIN-CONTAINING RNA-BINDING PROTEIN QKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16551">
        <property type="entry name" value="Quaking_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16544">
        <property type="entry name" value="STAR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="K Homology" evidence="20">
        <location>
            <begin position="118"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="379" mass="41243" checksum="53082556B8869561" modified="2019-09-18" version="1">MTNRPQVSDLAGGRGSPPLPPSPAAAAAAGGVSCGAWNMVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYF7</accession>
    <name>A0A4W2CYF7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-defensin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Has antibacterial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the beta-defensin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYF7"/>
    <dbReference type="SMR" id="A0A4W2CYF7"/>
    <dbReference type="STRING" id="30522.A0A4W2CYF7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029097.1">
        <property type="protein sequence ID" value="ENSBIXP00000016900.1"/>
        <property type="gene ID" value="ENSBIXG00000020980.1"/>
    </dbReference>
    <dbReference type="OMA" id="RCWNLHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.360.10">
        <property type="entry name" value="Antimicrobial Peptide, Beta-defensin 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050544">
        <property type="entry name" value="Beta-defensin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025933">
        <property type="entry name" value="Beta_defensin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001:SF3">
        <property type="entry name" value="BETA-DEFENSIN 123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001">
        <property type="entry name" value="BETA-DEFENSIN 123-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13841">
        <property type="entry name" value="Defensin_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="5 10" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="7 10" id="KW-0211">Defensin</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021493677" description="Beta-defensin" evidence="10">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-defensin" evidence="11">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU231113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="67" mass="7828" checksum="16B54329E92B2A0A" modified="2019-09-18" version="1">MKLLSLILAGLLLLSQLTPGGTRKCWNFHGKCRHKCFKKEKVYVYCTNNKLCCVKSRYLPKNLPWQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZ84</accession>
    <name>A0A4W2CZ84_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZ84"/>
    <dbReference type="SMR" id="A0A4W2CZ84"/>
    <dbReference type="STRING" id="30522.A0A4W2CZ84"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032080.1">
        <property type="protein sequence ID" value="ENSBIXP00000018816.1"/>
        <property type="gene ID" value="ENSBIXG00000022583.1"/>
    </dbReference>
    <dbReference type="OMA" id="MDPAMEY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10337">
        <property type="entry name" value="SH2_BCAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000007">
        <property type="entry name" value="SH2 domain containing 3C (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000013">
        <property type="entry name" value="SH2 domain-containing protein 3C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051853">
        <property type="entry name" value="SH2-Ras-GEF_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044102">
        <property type="entry name" value="SH2_SHEP1/BCAR3/NSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14247:SF10">
        <property type="entry name" value="BREAST CANCER ANTI-ESTROGEN RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14247">
        <property type="entry name" value="BREAST CANCER ANTI-ESTROGEN RESISTANCE PROTEIN 3 HOMOLOG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="154"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="7">
        <location>
            <begin position="549"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="348"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="826" mass="92737" checksum="9E49D48FF75E87AD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2R8</accession>
    <name>A0A4W2D2R8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. LISCH7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2R8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031606.1">
        <property type="protein sequence ID" value="ENSBIXP00000018509.1"/>
        <property type="gene ID" value="ENSBIXG00000004328.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070506">
        <property type="term" value="F:high-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051874">
        <property type="entry name" value="Ig-like_domain-LISCH7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008664">
        <property type="entry name" value="LISCH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923:SF3">
        <property type="entry name" value="IMMUNOGLOBULIN-LIKE DOMAIN-CONTAINING RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923">
        <property type="entry name" value="TRANSMEMBRANE AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05624">
        <property type="entry name" value="LSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="17"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="402"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="550" mass="63379" checksum="BC43D80853150D34" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3Z9</accession>
    <name>A0A4W2D3Z9_BOBOX</name>
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        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3Z9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046027.1">
        <property type="protein sequence ID" value="ENSBIXP00000013408.1"/>
        <property type="gene ID" value="ENSBIXG00000000681.1"/>
    </dbReference>
    <dbReference type="OMA" id="CVEASHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
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        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
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        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="521" mass="59972" checksum="9065CCD4F68CDF0A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7J9</accession>
    <name>A0A4W2D7J9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SCRAPIE-RESPONSIVE PROTEIN 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR17463">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021246873" evidence="1">
        <location>
            <begin position="21"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="98" mass="10952" checksum="3EC3465EB694C080" modified="2019-09-18" version="1">MKFTVLALAVGLTLLLGVQAMPANRLSCYRKILKDRNCHSLPEGVADLTKIDVNVQDHFWDGKGCEMVCYCNFSELLCCPKDVFFGPKVSFVIPCNNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8C9</accession>
    <name>A0A4W2D8C9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Decorin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Bone proteoglycan II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 16">May affect the rate of fibrils formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN. Interacts with DPT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14 16">Secreted</location>
            <location evidence="2 14 16">Extracellular space</location>
            <location evidence="2 14 16">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14 16">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8C9"/>
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        <property type="gene ID" value="ENSBIXG00000004758.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000038">
        <property type="entry name" value="Biglycan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016352">
        <property type="entry name" value="SLRP_I_decor/aspor/byglycan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF14">
        <property type="entry name" value="DECORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002490">
        <property type="entry name" value="SLRP_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14 16" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14 16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021511165" description="Decorin" evidence="14 16">
        <location>
            <begin position="17"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="17">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="55"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR002490-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="360" mass="39879" checksum="F7B5B61C2A67CB5E" modified="2019-09-18" version="1">MKATIIFLLVAQVSWAGPFQQKGLFDFMLEDEASGIGPEEHFPEVPEIEPMGPVCPFRCQCHLRVVQCSDLGLEKVPKDLPPDTALLDLQNNKITEIKDGDFKNLKNLHTLILINNKISKISPGAFAPLVKLERLYLSKNQLKELPEKMPKTLQELRVHENEITKVRKSVFNGLNQMIVVELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITTIPQGLPPSLTELHLDGNKITKVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLNNNKLVKVPGGLADHKYIQVVYLHNNNISAIGSNDFCPPGYNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRAAVQLGNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9I3</accession>
    <name>A0A4W2D9I3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cannabinoid receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CNR2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376273.1">
        <property type="nucleotide sequence ID" value="XM_027520472.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027376276.1">
        <property type="nucleotide sequence ID" value="XM_027520475.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027376282.1">
        <property type="nucleotide sequence ID" value="XM_027520481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9I3"/>
    <dbReference type="SMR" id="A0A4W2D9I3"/>
    <dbReference type="STRING" id="30522.A0A4W2D9I3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037882.1">
        <property type="protein sequence ID" value="ENSBIXP00000022662.1"/>
        <property type="gene ID" value="ENSBIXG00000007505.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054412.1">
        <property type="protein sequence ID" value="ENSBIXP00005000338.1"/>
        <property type="gene ID" value="ENSBIXG00005030340.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879181"/>
    <dbReference type="CTD" id="1269"/>
    <dbReference type="GeneTree" id="ENSGT01140000282530"/>
    <dbReference type="OMA" id="AFDRYIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004949">
        <property type="term" value="F:cannabinoid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030595">
        <property type="term" value="P:leukocyte chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099553">
        <property type="term" value="P:trans-synaptic signaling by endocannabinoid, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001551">
        <property type="entry name" value="Canbinoid_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002230">
        <property type="entry name" value="Cnbnoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00523">
        <property type="entry name" value="CANABINOID2R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00362">
        <property type="entry name" value="CANNABINOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="50"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="361" mass="40033" checksum="E0BDA311DFCBB9A9" modified="2019-09-18" version="1">MEICLKIEAANGSSDGLNFNPMKEYMILSGPQKIAIAVLCTLLGLLSALENLVVLYLIGSSHRLRKKPSYLFIGSLAGADFLASVVFASSFVHFHVFDGVDSKAVFLLKIGSVTLTFTASLGSLLLTAIDRYLCLRYPPTYKALLTRRRALVTLGIMWVLAALVSYLPLMGWTCCPRPCSELFPLIPNDYLLGWLLFIAALFAGIIYTYAHVLWKAHQHVASLAEHRDRHLSGIARMRLDVRLAKTLGMLLAVLFIFWFPVLALMVYSLGARLSDQVKKVFAFCSLLCLVNSMVNPIIYALRSGEIRSSAHHRLARWKKCVRGLGPEGKGEIPRSSVTETEADVKTTPGLDSRELSWPDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA20</accession>
    <name>A0A4W2DA20_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Metaxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MTX3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA20"/>
    <dbReference type="SMR" id="A0A4W2DA20"/>
    <dbReference type="STRING" id="30522.A0A4W2DA20"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049809.1">
        <property type="protein sequence ID" value="ENSBIXP00000021144.1"/>
        <property type="gene ID" value="ENSBIXG00000024299.1"/>
    </dbReference>
    <dbReference type="OMA" id="ANYFFGN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03212">
        <property type="entry name" value="GST_C_Metaxin1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03078">
        <property type="entry name" value="GST_N_Metaxin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017410">
        <property type="entry name" value="Metaxin1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF30">
        <property type="entry name" value="METAXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038150">
        <property type="entry name" value="Metaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01180">
        <property type="entry name" value="SUF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="11">
        <location>
            <begin position="173"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="278"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="35133" checksum="6D1F2882BA2D89FB" modified="2019-09-18" version="1">MAAPLELSCWGGGWGLPSVHSDSLVVMAYAKFSGAPLKVNVIDNNWRGSRGDVPVLTTEDNTISQPAKILNFLRKQKYNADYELSAKQGADTLAYIALLEEKLLPAVLHTFWVESDNYFTVTKPWFASRMPFPLSLILPGRMSKGALNRILLTRGEPPFYHLREVEAQIYRDAKECLNLLSNRLGTSQFFFGDMPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDILNSYFRVSLGGISPAGQETVDANLQKLTQLVNKESNLIEKMDDNLRQSPQLPPRKLPTLKLTPAEEESNSLHRLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCT4</accession>
    <name>A0A4W2DCT4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Securin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the securin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCT4"/>
    <dbReference type="STRING" id="30522.A0A4W2DCT4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037089.1">
        <property type="protein sequence ID" value="ENSBIXP00000022105.1"/>
        <property type="gene ID" value="ENSBIXG00000006288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006940">
        <property type="entry name" value="Securin_separation_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10418:SF2">
        <property type="entry name" value="SECURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10418">
        <property type="entry name" value="SECURIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04856">
        <property type="entry name" value="Securin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="194" mass="22547" checksum="97FB7D984097A6E0" modified="2019-09-18" version="1">LKTLIFYIYIYIYTHTCIYEPGTHVASKDRLNMGSVPSVKALDGRSQISMPRVDKCLMLHQLYQKLPERLWELSTENRQLSLPKRLLRRLKAKSYVPASDDTYSEIEKFFPFNPLDFESFNLPEEHQMQHLPLNGVPLMILDEERELEQLLHVGPFSPLKMSPPLWESNLLQSLSSILSTLDVELPPVCYDLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DE64</accession>
    <name>A0A4W2DE64_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRELI domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PRELID2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027401888.1">
        <property type="nucleotide sequence ID" value="XM_027546087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DE64"/>
    <dbReference type="SMR" id="A0A4W2DE64"/>
    <dbReference type="STRING" id="30522.A0A4W2DE64"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040471.1">
        <property type="protein sequence ID" value="ENSBIXP00000024371.1"/>
        <property type="gene ID" value="ENSBIXG00000026554.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023510.1">
        <property type="protein sequence ID" value="ENSBIXP00005036221.1"/>
        <property type="gene ID" value="ENSBIXG00005017702.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895208"/>
    <dbReference type="CTD" id="153768"/>
    <dbReference type="GeneTree" id="ENSGT00950000182810"/>
    <dbReference type="OMA" id="CRNVVPE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="177" mass="20485" checksum="354713FBFA5CADD2" modified="2019-09-18" version="1">MGVTVDVHEVFKYPFEQVVASFLRKYPNPMDKNVISVKIVEERKDVSTGIIYRKRIAVCQNVVPEILRKVNILKVPNIQLEEESWFNPQERNMAIRSHCLTWTQYASMKEESVFRESTENPNWTEFIQKGRISITGAGFLNCVLESFARSFLRQGVQKGIRIMEMLLKEQCGAPLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEG8</accession>
    <name>A0A4W2DEG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclosome subunit 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ANAPC13</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the APC13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401347.1">
        <property type="nucleotide sequence ID" value="XM_027545546.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401357.1">
        <property type="nucleotide sequence ID" value="XM_027545556.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401368.1">
        <property type="nucleotide sequence ID" value="XM_027545567.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401376.1">
        <property type="nucleotide sequence ID" value="XM_027545575.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401385.1">
        <property type="nucleotide sequence ID" value="XM_027545584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEG8"/>
    <dbReference type="SMR" id="A0A4W2DEG8"/>
    <dbReference type="STRING" id="30522.A0A4W2DEG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040663.1">
        <property type="protein sequence ID" value="ENSBIXP00000024491.1"/>
        <property type="gene ID" value="ENSBIXG00000026634.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038363.1">
        <property type="protein sequence ID" value="ENSBIXP00005042743.1"/>
        <property type="gene ID" value="ENSBIXG00005004478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894978"/>
    <dbReference type="CTD" id="25847"/>
    <dbReference type="GeneTree" id="ENSGT00390000008673"/>
    <dbReference type="OMA" id="PHDDIAV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141198">
        <property type="term" value="P:protein branched polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28672">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28672:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05839">
        <property type="entry name" value="Apc13p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="74" mass="8521" checksum="F3C52A7E3821CB3B" modified="2019-09-18" version="1">MDSEVQRDGRILDLIDDAWREDKLPYEDVAIPLNELPEPEQDNGGTTESVKEQEMKWTDLALQYLHENVPPIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEI1</accession>
    <name>A0A4W2DEI1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113888813</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEI1"/>
    <dbReference type="STRING" id="30522.A0A4W2DEI1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051630.1">
        <property type="protein sequence ID" value="ENSBIXP00000024506.1"/>
        <property type="gene ID" value="ENSBIXG00000026643.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030883">
        <property type="term" value="F:endogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030884">
        <property type="term" value="F:exogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071723">
        <property type="term" value="F:lipopeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048006">
        <property type="term" value="P:antigen processing and presentation, endogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048007">
        <property type="term" value="P:antigen processing and presentation, exogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21029">
        <property type="entry name" value="IgC1_CD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000254">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000002">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF175">
        <property type="entry name" value="ANTIGEN-PRESENTING GLYCOPROTEIN CD1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="268"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="418" mass="46972" checksum="5392AB5F610BD3BE" modified="2019-09-18" version="1">MSGTGDSRKRSARGSRAGKGEDARGGRAQTDAPRKAGSLIQQLENLLLPASSCLAPGDRARFRASRHHPFFPNLTVSFFLFFFLFPSVLSLPFPRLLGSVFSPDPQTPFSFHGLQISSLANSSWTRTDCLGWLGELQPYTWRNESDTIRFLKPWSRGTFSDQQWEQLQHTFQVYRSSFTKVLWEFVKRLHAELPLEIQGSAGCELLQGNTSESFLRAAFQGRDVLSFQGMSWVSAPDAPPWVQEVCKVINLDQGTKETVHWLLHDICPELVRGLLQTGKSELEKQVKPEAWLSSGPSPGPGRLLLVCHVSGFYPKPVRVMWMKGEQEEPGTRQGDVMPNVDSTWYLRVTLDVAAGEAAGLSCQVKHSSLGDQDIILYWDGNHVSRGLIVILVLLVFVLLFVGGLVFWFRKHRRYQDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEZ3</accession>
    <name>A0A4W2DEZ3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Prenylcysteine oxidase 1 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PCYOX1L</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prenylcysteine oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEZ3"/>
    <dbReference type="STRING" id="30522.A0A4W2DEZ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040966.1">
        <property type="protein sequence ID" value="ENSBIXP00000024681.1"/>
        <property type="gene ID" value="ENSBIXG00000026809.1"/>
    </dbReference>
    <dbReference type="OMA" id="NMWAVEG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001735">
        <property type="term" value="F:prenylcysteine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030327">
        <property type="term" value="P:prenylated protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030328">
        <property type="term" value="P:prenylcysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000081">
        <property type="entry name" value="prenylcysteine oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010795">
        <property type="entry name" value="Prenylcys_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017046">
        <property type="entry name" value="Prenylcysteine_Oxase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944">
        <property type="entry name" value="FARNESYLCYSTEINE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944:SF2">
        <property type="entry name" value="PRENYLCYSTEINE OXIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07156">
        <property type="entry name" value="Prenylcys_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036292">
        <property type="entry name" value="Prenylcysteine_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Prenylcysteine lyase" evidence="10">
        <location>
            <begin position="199"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR036292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="572" mass="62878" checksum="28E6578730AE415D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFV0</accession>
    <name>A0A4W2DFV0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Fc gamma receptor IIb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FCGR2B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027383360.1">
        <property type="nucleotide sequence ID" value="XM_027527559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041461.1">
        <property type="protein sequence ID" value="ENSBIXP00000025006.1"/>
        <property type="gene ID" value="ENSBIXG00000027135.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027686.1">
        <property type="protein sequence ID" value="ENSBIXP00005016364.1"/>
        <property type="gene ID" value="ENSBIXG00005020073.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883646"/>
    <dbReference type="CTD" id="2213"/>
    <dbReference type="GeneTree" id="ENSGT01050000244808"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019864">
        <property type="term" value="F:IgG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019770">
        <property type="term" value="F:IgG receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001788">
        <property type="term" value="P:antibody-dependent cellular cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050766">
        <property type="term" value="P:positive regulation of phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032760">
        <property type="term" value="P:positive regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05753">
        <property type="entry name" value="Ig2_FcgammaR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000217">
        <property type="entry name" value="High affinity immunoglobulin gamma Fc receptor I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000356">
        <property type="entry name" value="Low affinity immunoglobulin gamma Fc region receptor III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF97">
        <property type="entry name" value="LOW AFFINITY IMMUNOGLOBULIN GAMMA FC REGION RECEPTOR II-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0390">IgG-binding protein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610774" evidence="14">
        <location>
            <begin position="43"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="48"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="144"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="297" mass="33091" checksum="8B0F632A3D66A0F0" modified="2019-09-18" version="1">MGIPSFLAFPAARRNRAHCTPWHPWGHMLLWTALLFLAPVSGKPADLPKAVVTIQPAWINVLREDHVTLTCQGTSFSAGNLTTWFHNGSSIHTQKQPSYSFRAGSNDSGSYRCQREQTSLSDPVHLDVISDWLLLQTPSLVFQEGEPIMLRCHSWRNQPLNKITFYQDRKSKIFSYQRTNFSIPRANLSHSGQYHCTAFIGKMLHSSQPVNITVQESSSSGPSSMTAVAIGTCFAAVAIVAAIITWFRLRRKPISAGLTDAENDAARTEAENTVTYSLLSHPDVAEEDSESDYQKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DHF7</accession>
    <name>A0A4W2DHF7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Family with sequence similarity 241 member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FAM241A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM241 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399628.1">
        <property type="nucleotide sequence ID" value="XM_027543827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DHF7"/>
    <dbReference type="SMR" id="A0A4W2DHF7"/>
    <dbReference type="STRING" id="30522.A0A4W2DHF7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042381.1">
        <property type="protein sequence ID" value="ENSBIXP00000025624.1"/>
        <property type="gene ID" value="ENSBIXG00000027704.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051983.1">
        <property type="protein sequence ID" value="ENSBIXP00005023637.1"/>
        <property type="gene ID" value="ENSBIXG00005007640.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894022"/>
    <dbReference type="CTD" id="132720"/>
    <dbReference type="GeneTree" id="ENSGT00940000154340"/>
    <dbReference type="OMA" id="MCSAGQL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027953">
        <property type="entry name" value="DUF4605"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052502">
        <property type="entry name" value="FAM241_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33690">
        <property type="entry name" value="DUF4605 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33690:SF1">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 241 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15378">
        <property type="entry name" value="DUF4605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4605" evidence="8">
        <location>
            <begin position="79"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="138" mass="15070" checksum="3A999FB398573F54" modified="2019-09-18" version="1">MCSAGQLLGGGGGGGGSGGERDEDRDALAERAAAGTEQESGASPRRRGRRPLEEREQDIEESQNHTGEPVGDDYKKMGTLFGELNKSLLNMGFTRMYFGEQIVEPVIVIFFWVMLWFLGLPAFGLVALLCLVIIYVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKC7</accession>
    <name>A0A4W2DKC7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytosolic Fe-S cluster assembly factor NUBP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nucleotide-binding protein 2</fullName>
            <shortName evidence="12">NBP 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EME2</name>
        <name evidence="12" type="synonym">NUBP2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytoskeleton</location>
            <location evidence="12">Microtubule organizing center</location>
            <location evidence="12">Centrosome</location>
        </subcellularLocation>
        <text evidence="12">Enriched at the centrosomes during mitosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKC7"/>
    <dbReference type="STRING" id="30522.A0A4W2DKC7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015304.1">
        <property type="protein sequence ID" value="ENSBIXP00000026850.1"/>
        <property type="gene ID" value="ENSBIXG00000029288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000796">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03039">
        <property type="entry name" value="NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028600">
        <property type="entry name" value="NUBP2/Cfd1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="377"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="445" mass="46765" checksum="A2D0EAF310016D2C" modified="2019-09-18" version="1">MARPGPGRAGSARRGLGERRPPTWEISDSDAEGPAGVETPARGRDPAEERRPAAEALRRLRPGQAVRRLAVLVDPEPGNLAGVRHIVLVLSGKGGVGKSTISTELALALRHAGKKVGILDVDLCGPSIPRMLRAQGRAVHQGDSGWVPVFVDREQSISLMSVGFLLEQPDEAVVWRGPKKNALIKQFVSDVAWGQLDYLLVDTPPGTSDEHMAVVDALRPHSPLGALVVTTPQAVSVGDVRRELTFCRKVGLRVIGLVENMSGFVCPHCSECTNVFSKGGGEELARHAGVPFLGEWAGAATGPCPPQGEGASSHPADSPPRESCQPQAPCAFFCRSTREAAGPAGWLLGVLGRLSPGMGPAGPRQCCPRAPGLRGWTGPPSLALTAHSPPPPRLGAPGPRAHAEPGGWPGLHPGLPRQPRLPRALLHRPEDSQRDACGALLTEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DND6</accession>
    <name>A0A4W2DND6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in the correct development and proper functioning of the peripheral and central nervous system and be involved in cell adhesion and intercellular communication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2DND6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042404.1">
        <property type="protein sequence ID" value="ENSBIXP00000025646.1"/>
        <property type="gene ID" value="ENSBIXG00000027729.1"/>
    </dbReference>
    <dbReference type="OMA" id="GWNCGRE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000005">
        <property type="entry name" value="Contactin associated protein-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000026">
        <property type="entry name" value="contactin-associated protein-like 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF46">
        <property type="entry name" value="CONTACTIN-ASSOCIATED PROTEIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021471524" evidence="14">
        <location>
            <begin position="27"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1238"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="15">
        <location>
            <begin position="30"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="16">
        <location>
            <begin position="180"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="16">
        <location>
            <begin position="367"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="546"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="18">
        <location>
            <begin position="582"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="16">
        <location>
            <begin position="791"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="957"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="16">
        <location>
            <begin position="1013"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1306" mass="145721" checksum="8325C8F2E6B6563D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQ68</accession>
    <name>A0A4W2DQ68_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein polybromo-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">BRG1-associated factor 180</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin. Interacts with PHF10/BAF45A. Interacts with acetylated 'Lys-14' of histone H3 (H3K14ac), and may also interact with other acetylated or methylated Lys residues on histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2DQ68"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053890.1">
        <property type="protein sequence ID" value="ENSBIXP00000026288.1"/>
        <property type="gene ID" value="ENSBIXG00000030305.1"/>
    </dbReference>
    <dbReference type="OMA" id="WQFYETL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05524">
        <property type="entry name" value="Bromo_polybromo_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05517">
        <property type="entry name" value="Bromo_polybromo_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05520">
        <property type="entry name" value="Bromo_polybromo_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05518">
        <property type="entry name" value="Bromo_polybromo_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05515">
        <property type="entry name" value="Bromo_polybromo_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05526">
        <property type="entry name" value="Bromo_polybromo_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21984">
        <property type="entry name" value="HMG-box_PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000009">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000011">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000013">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000006">
        <property type="entry name" value="protein polybromo-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000010">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000015">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000003">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000002">
        <property type="entry name" value="Rap guanine nucleotide exchange factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000012">
        <property type="entry name" value="Rap guanine nucleotide exchange factor like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF230">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="429"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="667" mass="73798" checksum="9C60DE8342E4D0A3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E516</accession>
    <name>A0A4W2E516_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Telomerase-binding protein EST1A</fullName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SMG6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).</text>
    </comment>
    <comment type="function">
        <text evidence="12">Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">May form homooligomers. Associated component of the telomerase holoenzyme complex. Interacts with TERT, independently of the telomerase RNA. Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits. Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay. Interacts with DHX34; the interaction is RNA-independent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Chromosome</location>
            <location evidence="2 15">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The PINc domain confers endonuclease activity and is expected to bind the catalytic metal ion.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="STRING" id="30522.A0A4W2E516"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015513.1">
        <property type="protein sequence ID" value="ENSBIXP00000026783.1"/>
        <property type="gene ID" value="ENSBIXG00000029414.1"/>
    </dbReference>
    <dbReference type="OMA" id="CSPDVWQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09885">
        <property type="entry name" value="PIN_Smg6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000094">
        <property type="entry name" value="telomerase-binding protein EST1A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000014">
        <property type="entry name" value="telomerase-binding protein EST1A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF0">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN EST1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 15" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="PIN" evidence="17">
        <location>
            <begin position="1397"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="180"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="341"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="968"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1005"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="341"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="411"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="968"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1570" mass="176343" checksum="A6FF6CC8387B2192" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5Q8</accession>
    <name>A0A4W2E5Q8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoinositide-3-kinase regulatory subunit 6</fullName>
        </submittedName>
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        <name evidence="1" type="primary">PIK3R6</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
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        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 6"/>
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        <property type="entry name" value="PI3K_1B_p101"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="756" mass="84011" checksum="C14AD8FFEBFAFA0C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6P8</accession>
    <name>A0A4W2E6P8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Syntaxin-1B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.</text>
    </comment>
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        <text evidence="11">Interacts with OTOF. Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the syntaxin family.</text>
    </comment>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:exocytic insertion of neurotransmitter receptor to postsynaptic membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
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        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="191"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35982" checksum="25BF837F68149B4C" modified="2019-09-18" version="1">MKDRTQELRSAKDSDDEEEVVHVDRDHFMDEFFEQVEEIRGCIEKLSEDVEQVKKQHSAILAAPNPDEKTKQELEDLTTDIKKTANKVRSKLKAIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMTEYNATQSKYRDRCKDRIQRQLEITGRTTTNEELEDMLESGKLAIFTDDIKMDSQMTKQALNEIETRHNEIIKLETSIRELHDMFVDMAMLVESQGEMIDRIEYNVEHSVDYVERAVSDTKKAVKYQSKARRPGYPLSPGVSGIPTALGPQEGWSHGWRDAAASLSGIRSVTAHLWASDGPKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8N8</accession>
    <name>A0A4W2E8N8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSBIXG00000014986.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN LIGHT CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48424:SF3">
        <property type="entry name" value="DYNEIN LIGHT CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="118" mass="13150" checksum="ACB9889E22FAF255" modified="2019-09-18" version="1">MLLTRFEQENGHLAQVEVDEVFGFMCHITTEVPPHDAVPGGIVLLVKLLLDMGRNVLLYIILFQCLSSTLHRVLLHLLRYIGILDYGLSVTHGYQGGRGSLVGCRLWGRTESDTTEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9H6</accession>
    <name>A0A4W2E9H6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Does not bind lactose, and may not bind carbohydrates.</text>
    </comment>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="F:carbohydrate binding"/>
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        <property type="entry name" value="GALECTIN-RELATED PROTEIN"/>
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        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
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        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="323"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="156"/>
        </location>
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        <location>
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            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="455" mass="48701" checksum="E126EF1DEFB4735F" modified="2019-09-18" version="1">MKAQRPFIIFIPWKVRNESFLALRLNSLKEISSSGIHLHLQSSLDLGLHCPTCPQAQPEKEGGGRRGGAGMSQAPVTWQAPASPPHPRPHPPCRRRGPTRRPPPRTPGHWQASPSRPSLLPPSNGRGAGQAEQAAAAQPGLRPVPGSRAGPRRPLNAALGRHGAALATFFLGRRQRHPEEESSLDSAAPSRPRRLPPTLGPAPPPPPLPTPPPPPPPGPAGPVGAGPPGVRARVPARVCAPQPASDPRLRPAQDRPGIPARASRPTSPAARPPAAHPAAPREKMAGSVADSDAVVKLDDGHLNNSLGSPVQADVYFPRLIVPFCGHIKGGMRPGKKVLVMGIVDLNPESFAISLTCGDSEDPPADVAIELKAVFTDRQLLRNSCISGERGEEQSAIPYFPFIPDQPFRVEILCEHPRFRVFVDGHQLFDFYHRIQTLSAIDTIKINGDLQITKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9Y5</accession>
    <name>A0A4W2E9Y5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nucleolar protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Apoptosis repressor with CARD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">NOL3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E9Y5"/>
    <dbReference type="STRING" id="30522.A0A4W2E9Y5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018445.1">
        <property type="protein sequence ID" value="ENSBIXP00000032840.1"/>
        <property type="gene ID" value="ENSBIXG00000015520.1"/>
    </dbReference>
    <dbReference type="OMA" id="MGNSQER"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:caspase binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:death effector domain binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:death receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090201">
        <property type="term" value="P:negative regulation of release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="nucleolar protein 3 isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052685">
        <property type="entry name" value="Apoptosis_Repressor_CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22797">
        <property type="entry name" value="CARD6/NUCLEOLAR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22797:SF37">
        <property type="entry name" value="NUCLEOLAR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CARD" evidence="19">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="111"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
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            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="206" mass="22577" checksum="FFA13008BCFE5757" modified="2019-09-18" version="1">MGNTQERPSETIDHERKRLVETLQADSGLLLDALLARGVLTGPEYEALDALPDAERRVRRLLLLVQSKGEAACQELLSCAQRTVRAPDPTWDWQHVGTGYRERSYDPPCPGHWTPGAADSGTTCPWLPRAPESDEARGPEDSEAAQSGTLEEAEPELEAETSEGAEPELDPQMDPEPEPEAEPEPEAEPEPEPEPDFEAGDESEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAN6</accession>
    <name>A0A4W2EAN6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calcium binding protein 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CAB39</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371771.1">
        <property type="nucleotide sequence ID" value="XM_027515970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAN6"/>
    <dbReference type="SMR" id="A0A4W2EAN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026968.1">
        <property type="protein sequence ID" value="ENSBIXP00000036505.1"/>
        <property type="gene ID" value="ENSBIXG00000020000.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876577"/>
    <dbReference type="CTD" id="51719"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000025">
        <property type="entry name" value="Calcium-binding protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF11">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="341" mass="39869" checksum="EC710A528B6F9811" modified="2019-09-18" version="1">MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQQNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSAEFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQAKLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPAQQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB33</accession>
    <name>A0A4W2EB33_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arginine and glutamate rich 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ARGLU1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARGLU1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EB33"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047152.1">
        <property type="protein sequence ID" value="ENSBIXP00000036659.1"/>
        <property type="gene ID" value="ENSBIXG00000020135.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035000.1">
        <property type="protein sequence ID" value="ENSBIXP00005021254.1"/>
        <property type="gene ID" value="ENSBIXG00005024261.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111249"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033371">
        <property type="entry name" value="ARGLU1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31711">
        <property type="entry name" value="ARGININE AND GLUTAMATE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31711:SF1">
        <property type="entry name" value="ARGININE AND GLUTAMATE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15346">
        <property type="entry name" value="ARGLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="255" mass="31099" checksum="2909CB6DB2624941" modified="2019-09-18" version="1">MGRSRSRSSSRSKHTKSSKHNKKRSRSRSRSRDKERVRKRSKSRESKRNRRRDRERASSPPDRIDIFGRTVSKRSSLDEKQKREEEEKKAEFERQRKIRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEELERQRQAELAAQKAREEEERAKREELERILEENNRKIAEAQAKLAEEQLRIVEEQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPKLSFSLKTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECD6</accession>
    <name>A0A4W2ECD6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Inter-alpha-trypsin inhibitor heavy chain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ITIH2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITIH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECD6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044928.1">
        <property type="protein sequence ID" value="ENSBIXP00000033588.1"/>
        <property type="gene ID" value="ENSBIXG00000016419.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01461">
        <property type="entry name" value="vWA_interalpha_trypsin_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000013">
        <property type="entry name" value="inter-alpha-trypsin inhibitor heavy chain H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010600">
        <property type="entry name" value="ITI_HC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF14">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06668">
        <property type="entry name" value="ITI_HC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021307325" evidence="8">
        <location>
            <begin position="19"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="VIT" evidence="10">
        <location>
            <begin position="45"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="299"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="935" mass="104996" checksum="8EC99148CC21BF45" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECF6</accession>
    <name>A0A4W2ECF6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034585.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="431" mass="47824" checksum="CD889A1EF6865485" modified="2019-09-18" version="1">MEFSERKRSRKSQSFKLVNRDYHHEVYKISDFSSDVNGEAKETQPIFLGDESMEIKKQITGMRRLLNDSAGRIYQRVGKEGEKLKEEPQDLDAIWPQRLNSAEAPPSLHPSSRGTWNELPPQSGQFSGQYGTRSRTFQSQPHTAGSSNGELPVVNSSAGSNCCTCNCQSTLQAILQELKTMRKLMQIQAVGTQNRQQPPISLICSQRTAVSRKRNKKKKVPPKTVEPLTVKQKPSALEAEKKAAGAPENSSLPVAEHASPGESHILGFGIVLESPASDPEVQLAEGFDVFMPKSQLDSILSNYTRSGSLLFRKLVCAFFDDKTLANSLPNGKRKRGLNDNRKGLDQNIVGAIKVFTEKYCTANHVDKLPGPRDWVQILQDQIKLARRRLKRGSAEAVDGDERLDSVSLTPTGHILDTRREKPEDTGTAFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EE60</accession>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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    <sequence length="405" mass="46966" checksum="6A079A388CCBF225" modified="2019-09-18" version="1">MEKKCKKAKWLSGEALQIAVKRREAKSKGEKERYKHLNAEFQRIARRDKKAFLSDQCKEIEENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITDLEPDILECEVKWALESITMNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYMNCELPDVQAGFRKGRGTRDQIANIHWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLICILRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFJ9</accession>
    <name>A0A4W2EFJ9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Low-density lipoprotein receptor-related protein 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. May act as a tumor suppressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">May interact with RACK1, ZFYVE9 and NMRK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <location evidence="13">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000035897.1"/>
        <property type="gene ID" value="ENSBIXG00000019230.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:neuron migration"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000024">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="low-density lipoprotein receptor-related protein 12"/>
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    </dbReference>
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        <property type="entry name" value="low-density lipoprotein receptor-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000003">
        <property type="entry name" value="Putative low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
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        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021483691" description="Low-density lipoprotein receptor-related protein 12" evidence="19">
        <location>
            <begin position="28"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="20">
        <location>
            <begin position="28"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="20">
        <location>
            <begin position="240"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="604"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="783"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="669"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="783"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="840" mass="93083" checksum="8BE9B798CBE745F2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGC6</accession>
    <name>A0A4W2EGC6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oxytocin-neurophysin 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OXT</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Neurophysin 1 specifically binds oxytocin.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the vasopressin/oxytocin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGC6"/>
    <dbReference type="STRING" id="30522.A0A4W2EGC6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026734.1">
        <property type="protein sequence ID" value="ENSBIXP00000036412.1"/>
        <property type="gene ID" value="ENSBIXG00000019885.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021032.1">
        <property type="protein sequence ID" value="ENSBIXP00005035139.1"/>
        <property type="gene ID" value="ENSBIXG00005016246.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004511"/>
    <dbReference type="OMA" id="ACVEENY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005185">
        <property type="term" value="F:neurohypophyseal hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031855">
        <property type="term" value="F:oxytocin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031894">
        <property type="term" value="F:V1A vasopressin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.9.10:FF:000001">
        <property type="entry name" value="oxytocin-neurophysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.9.10">
        <property type="entry name" value="Neurohypophysial hormone domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000981">
        <property type="entry name" value="Neurhyp_horm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036387">
        <property type="entry name" value="Neurhyp_horm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022423">
        <property type="entry name" value="Neurohypophysial_hormone_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681">
        <property type="entry name" value="NEUROPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681:SF2">
        <property type="entry name" value="OXYTOCIN-NEUROPHYSIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00220">
        <property type="entry name" value="Hormone_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00184">
        <property type="entry name" value="Hormone_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00831">
        <property type="entry name" value="NEUROPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00003">
        <property type="entry name" value="NH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49606">
        <property type="entry name" value="Neurophysin II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00264">
        <property type="entry name" value="NEUROHYPOPHYS_HORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="2" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="172" mass="17794" checksum="BC1C40976B20022E" modified="2019-09-18" version="1">MLSTSLFMAAMTNTPQRHRSAHAAAGLKRPDPRDGRSPRPGDQRVCTMAGSSLACCLLGLLALTSACYIQNCPLGGKRAVLDLDVRTCLPCGPGGKGRCFGPSICCGDELGCFVGTAEALRCQEENYLPSPCQSGQKPCGSGGRCAAAGICCSPDGCHEDPACDPEAAFSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGG8</accession>
    <name>A0A4W2EGG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HARBI1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027418289.1">
        <property type="nucleotide sequence ID" value="XM_027562488.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027418291.1">
        <property type="nucleotide sequence ID" value="XM_027562490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGG8"/>
    <dbReference type="STRING" id="30522.A0A4W2EGG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046295.1">
        <property type="protein sequence ID" value="ENSBIXP00000035500.1"/>
        <property type="gene ID" value="ENSBIXG00000018796.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025169.1">
        <property type="protein sequence ID" value="ENSBIXP00005036936.1"/>
        <property type="gene ID" value="ENSBIXG00005018637.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905351"/>
    <dbReference type="CTD" id="283254"/>
    <dbReference type="GeneTree" id="ENSGT00940000154348"/>
    <dbReference type="OMA" id="AEPNCKV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF253">
        <property type="entry name" value="NUCLEASE HARBI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="13">
        <location>
            <begin position="148"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="349" mass="39003" checksum="BD618E2CAE4DBC1D" modified="2019-09-18" version="1">MAIPITVLDCDLLLYGRGHRTLDRFKLDDVTDEYLMSMYGFPRQFIYYLVELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNRKGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWLLGDSSFFLRTWLMTPLHIPETPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKSSHIILACCVLHNISLEHGMDVWSSPVTGPVEQPPEEEYEHMESLDLEADRIRQELMLTHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHL6</accession>
    <name>A0A4W2EHL6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="gene ID" value="ENSBIXG00000020716.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="L1-EN"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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        <property type="entry name" value="RT_dom"/>
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        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNase I-like"/>
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        <property type="entry name" value="RT_POL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="497"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1272" mass="149524" checksum="9474FCD118D9743F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKH6</accession>
    <name>A0A4W2EKH6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="11">Netrin-5</fullName>
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        <alternativeName>
            <fullName evidence="12">Netrin-1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NTN5</name>
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    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in neurogenesis. Prevents motor neuron cell body migration out of the neural tube.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027371029.1">
        <property type="nucleotide sequence ID" value="XM_027515228.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027515229.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2EKH6"/>
    <dbReference type="STRING" id="30522.A0A4W2EKH6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023507.1">
        <property type="protein sequence ID" value="ENSBIXP00000034870.1"/>
        <property type="gene ID" value="ENSBIXG00000017972.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038847.1">
        <property type="protein sequence ID" value="ENSBIXP00005023831.1"/>
        <property type="gene ID" value="ENSBIXG00005026518.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876244"/>
    <dbReference type="CTD" id="126147"/>
    <dbReference type="GeneTree" id="ENSGT00940000161874"/>
    <dbReference type="OMA" id="DPCYDPQ"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="NTR_netrin-1_like"/>
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    </dbReference>
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        <property type="entry name" value="Netrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000048">
        <property type="entry name" value="Netrin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000020">
        <property type="entry name" value="Netrin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
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        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
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        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF262">
        <property type="entry name" value="NETRIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611124" description="Netrin-5" evidence="15">
        <location>
            <begin position="17"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="275"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="17">
        <location>
            <begin position="345"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="489" mass="53180" checksum="94ED61179EAC16F2" modified="2019-09-18" version="1">MPVTFALWLLLSQASADPCYHPGGRPRFCLPPVTQLAGMAASCPQACGPSPGVDLGPRAPCNGSLTLGLGGPFLLSSVSLRFCTPGPPALVLSAAWATGGPWRSLWRRPAWPGVLGGPERVIFRVPAGPKASVVASHLRLELGGRGGLAAAGVRGRCQCHGHAARCAARDRPPRCRCRHHTTGPGCESCQPSHRDWPWRPATPRHPHPCLPCSCNQHARRCRFNSELFRLSGGRSGGVCERCRHHTAGRHCHYCQPGFWRDPGQPISSRKACRACQCHPIGATGGACNQTNGQCSCKLGVTGLTCNRCGPGYQQSRSPRMPCQRILEATTTLATTPHAYSSDPQCQNYCNTSDTRIHMSLQRYCQQDYVLHAQVLASEAADTTWQRLAVRVLAVYKQGAQPVRRGSQAAWVPRADLTCGCLRLRPASHYLLLGRTAGGPDPTRLVLDRHGLALPWRPRWARPLRRLQQEERAGGCRTLRPSTLSPEPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKR1</accession>
    <name>A0A4W2EKR1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anillin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Bleb</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKR1"/>
    <dbReference type="STRING" id="30522.A0A4W2EKR1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024170.1">
        <property type="protein sequence ID" value="ENSBIXP00000035309.1"/>
        <property type="gene ID" value="ENSBIXG00000018558.1"/>
    </dbReference>
    <dbReference type="OMA" id="PKIEQQT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032059">
        <property type="term" value="C:bleb"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000915">
        <property type="term" value="P:actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01263">
        <property type="entry name" value="PH_anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000111">
        <property type="entry name" value="anillin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012966">
        <property type="entry name" value="AHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031970">
        <property type="entry name" value="Anillin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037840">
        <property type="entry name" value="PH_Anillin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF27">
        <property type="entry name" value="ANILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08174">
        <property type="entry name" value="Anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16018">
        <property type="entry name" value="Anillin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="456"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="631"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="458"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="475"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="638"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="661"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00057106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1129" mass="124411" checksum="BB724833173B6E0B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EL24</accession>
    <name>A0A4W2EL24_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Espin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Ectoplasmic specialization protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells. Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia. Involved in the elongation of actin in stereocilia. In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EL24"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046563.1">
        <property type="protein sequence ID" value="ENSBIXP00000035885.1"/>
        <property type="gene ID" value="ENSBIXG00000003407.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000174">
        <property type="entry name" value="Espin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052420">
        <property type="entry name" value="Espin/Espin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24153">
        <property type="entry name" value="ESPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24153:SF14">
        <property type="entry name" value="ESPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1009">Hearing</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="205"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="18">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="334"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="798"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="350"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="PROSITE" id="PS51082"/>
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        <source>
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        <source>
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    </evidence>
    <sequence length="852" mass="91819" checksum="BB2505DE74A05502" modified="2019-09-18" version="1"> </sequence>
</entry>
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    <accession>A0A4W2ELV7</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        </source>
    </evidence>
    <sequence length="408" mass="46898" checksum="D7BAA809DEBFAA01" modified="2019-09-18" version="1">MLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANICWIIEKAREFQKNIYFCFIDYAKAFDCVHHNKLWKILKEMGIPDHLICLLRNLYAGQEAAVRTEHGTTDWFQIGKGIHQGCILSPCLFNLYAEYTMRNAGLEETKAGIKIAGRNIYNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSDFVFLGSKITADGDCSLEIKRCLLLGRKVMTNLDSIFKSRDITLPTKVHLVKDMVFPVVLYGCENWTVKKAECRRIDAFELWCWRRLLRVPWTARRSNQPILKEISPGISLEGMMLKLKLQYFGPLMRRVDSLEKTLMLGGIGGRRRRGQQRMRWLDGITDSMDVSLGELQELVMDREAWRAAIYGVAKSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQK5</accession>
    <name>A0A4W2EQK5_BOBOX</name>
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        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member A3</fullName>
        </submittedName>
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        <name evidence="7" type="primary">SLC35A3</name>
    </gene>
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    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="37"/>
            <end position="59"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="249"/>
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        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039026.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="285" mass="31493" checksum="622C065684A009CA" modified="2019-09-18" version="1">MSANLKYLSLGILVFQTTSLVLTMRYSRTLKEEGPRYLSSTAVVVAELLKIMACILLVYKDSKCSLRALNRILHDEILNKPMETLKLAIPSGIYTLQNNLLYVALSNLDAATYQWPSDSQELNSKELSAGSQFVGLMAVLTACFSSGFAGVYFEKILKETKQSVWIRNIQLGFFGSIFGLMGVYVYDGELVSKNGFFQGYNRLTWIVVVLQALGGLVIAAVIKYADNILKGFATSLSIILSTLISYFWLQDFVPTSVFFLGAILVITATFLYGYDPKPAGNPTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ER57</accession>
    <name>A0A4W2ER57_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113885398</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ER57"/>
    <dbReference type="STRING" id="30522.A0A4W2ER57"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033315.1">
        <property type="protein sequence ID" value="ENSBIXP00000039259.1"/>
        <property type="gene ID" value="ENSBIXG00000023196.1"/>
    </dbReference>
    <dbReference type="OMA" id="HYHIIMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF166">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="46"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="317" mass="35178" checksum="38354CFC033CF34D" modified="2019-09-18" version="1">MAIMNGDARNRTAAPEFTLEGFPAVQHLGKVLFSVHLLMYLASVTGNTLIITITWADHHLQTPMYFFLSSFSFLECCFTTTVIPKLLAIFLLGRQKISFAACITQASVFLFLGATVFFLMAVLSLDRYMAICKPLYYPTIMNPRVCSLLVSACLVLGFSLMVVPVTKLSQLSFCGSRVIPHFFCDLGPLINLSCSDIRSIEMLAFGLALFILSTSFIITIISYSNIVVTILHLPSAKERQKAFSTCSSHFIVLSLMYGSCVFIYMKPKGTNQLDSNKGAALVNTVVTPLLNPVIYTLRNKQVHQALRNALTRVRLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUH4</accession>
    <name>A0A4W2EUH4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EUH4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036446.1">
        <property type="protein sequence ID" value="ENSBIXP00000040579.1"/>
        <property type="gene ID" value="ENSBIXG00000024664.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009101">
        <property type="term" value="P:glycoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000807">
        <property type="entry name" value="galactosylceramide sulfotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF55">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="399" mass="46202" checksum="7C068652B6DC8892" modified="2019-09-18" version="1">MPSFLGGLQRCFWAVLLLLALAVLLLGCIMHVDIGLLMPQLGDQADGPPVTNVMFLKTHKTASSTVLNILYRFAETHNLSAALPAGGRFHLGYPWLFLARYVEGAEQGGPRQRFNIMCNHLRFNPPEVQKVMHNDTFYFSILRNPVFQLESSFVYYKSHVPAFRNVSSLDAFLASPWTYYNQSLGLSNAHARNSMWFDLGFDNDAPPEEGYVRARLLDVEQRFQLLLIAEHFDESMVLLRRLLRWRLDDVVAFRLNSRSQHSVTPLSPAGQERAKHWCALDWRLYQHFNRTFWARLRAELSPRRLRSEVARLRERRRELAAMCLQDSEPKNQSQITDFRLRPYQSGRADILGYNLRPGLDNQTLQMCQRMVMPELQYMAHLYTLQFPDKPPKNIAFLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZM5</accession>
    <name>A0A4W2EZM5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Multimerin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MMRN1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Multimeric. Composed of varying sized, disulfide-linked multimers, the smallest of which is a homotrimer. Proteolysis of the promultimerin in the N-terminal region, leads to the mature p155 form that is stored in platelets. Interacts with factor V/Va.</text>
    </comment>
    <comment type="subcellular location">
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with DLEC1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410687.1">
        <property type="nucleotide sequence ID" value="XM_027554886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FDX9"/>
    <dbReference type="SMR" id="A0A4W2FDX9"/>
    <dbReference type="STRING" id="30522.A0A4W2FDX9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046409.1">
        <property type="protein sequence ID" value="ENSBIXP00000014234.1"/>
        <property type="gene ID" value="ENSBIXG00000000595.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009926.1">
        <property type="protein sequence ID" value="ENSBIXP00005003633.1"/>
        <property type="gene ID" value="ENSBIXG00005009807.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901043"/>
    <dbReference type="CTD" id="10574"/>
    <dbReference type="GeneTree" id="ENSGT00550000074832"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032212">
        <property type="term" value="P:positive regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="543" mass="59443" checksum="E5367265CFAC03EF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FI61</accession>
    <name>A0A4W2FI61_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Stomatin-like protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">STOML2</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405372.1">
        <property type="nucleotide sequence ID" value="XM_027549571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FI61"/>
    <dbReference type="SMR" id="A0A4W2FI61"/>
    <dbReference type="STRING" id="30522.A0A4W2FI61"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026550.1">
        <property type="protein sequence ID" value="ENSBIXP00000015229.1"/>
        <property type="gene ID" value="ENSBIXG00000019789.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007749.1">
        <property type="protein sequence ID" value="ENSBIXP00005004737.1"/>
        <property type="gene ID" value="ENSBIXG00005010632.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897160"/>
    <dbReference type="CTD" id="30968"/>
    <dbReference type="GeneTree" id="ENSGT01030000234614"/>
    <dbReference type="OMA" id="YLQMLPK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901612">
        <property type="term" value="F:cardiolipin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042608">
        <property type="term" value="F:T cell receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035710">
        <property type="term" value="P:CD4-positive, alpha-beta T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010876">
        <property type="term" value="P:lipid localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032743">
        <property type="term" value="P:positive regulation of interleukin-2 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990046">
        <property type="term" value="P:stress-induced mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000008">
        <property type="entry name" value="Stomatin-like protein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="STML2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="7">
        <location>
            <begin position="36"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="356" mass="38733" checksum="EA8307A9056CC983" modified="2019-09-18" version="1">MLARAARGTGALLLKGSVQASARAPRRASSGLPRNTVVLFVPQQEAWVVERMGRFHRILEPGLNILIPVLDRIRYVQSLKEIVINVPEQSAVTLDNVTLQIDGVLYLRIMDPYKASYGVEDPEYAVTQLAQTTMRSELGKLSLDKVFRERESLNASIVDAINQAADCWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGTRESAINVAEGKKQAQILASEAEKAEQINQAAGEASAVLAKAKAKAEAIRILAAALTQHNGDAAASLTVAEQYVSAFSKLAKDSNTILLPSNPGDVTSMVAQAMGVYGALTKAPIPEAQDSVSSRSSRDVRSTDASLDEELDRVKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FR72</accession>
    <name>A0A4W2FR72_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related protein Rab-25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RAB25</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB25 regulates epithelial cell differentiation, proliferation and survival, thereby playing key roles in tumorigenesis. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts (GTP-bound form) with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Interacts with VPS33B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Pseudopodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386417.1">
        <property type="nucleotide sequence ID" value="XM_027530616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FR72"/>
    <dbReference type="SMR" id="A0A4W2FR72"/>
    <dbReference type="STRING" id="30522.A0A4W2FR72"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040098.1">
        <property type="protein sequence ID" value="ENSBIXP00000042330.1"/>
        <property type="gene ID" value="ENSBIXG00000001478.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001241.1">
        <property type="protein sequence ID" value="ENSBIXP00005007994.1"/>
        <property type="gene ID" value="ENSBIXG00005001504.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885338"/>
    <dbReference type="CTD" id="57111"/>
    <dbReference type="GeneTree" id="ENSGT00940000158230"/>
    <dbReference type="OMA" id="KRACCIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031260">
        <property type="term" value="C:pseudopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031489">
        <property type="term" value="F:myosin V binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003382">
        <property type="term" value="P:epithelial cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010634">
        <property type="term" value="P:positive regulation of epithelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031268">
        <property type="term" value="P:pseudopodium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="213" mass="23537" checksum="29ECDCE77E266B9B" modified="2019-09-18" version="1">MGNRAEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAIKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQSREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNNARTNAVTLGSGPAGQELGPGEKRACCISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FSL1</accession>
    <name>A0A4W2FSL1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box J2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FOXJ2</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027397038.1">
        <property type="nucleotide sequence ID" value="XM_027541237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FSL1"/>
    <dbReference type="STRING" id="30522.A0A4W2FSL1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044191.1">
        <property type="protein sequence ID" value="ENSBIXP00000009411.1"/>
        <property type="gene ID" value="ENSBIXG00000015137.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016440.1">
        <property type="protein sequence ID" value="ENSBIXP00005008765.1"/>
        <property type="gene ID" value="ENSBIXG00005013812.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892412"/>
    <dbReference type="CTD" id="55810"/>
    <dbReference type="GeneTree" id="ENSGT00940000161053"/>
    <dbReference type="OMA" id="QGPHLYP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007141">
        <property type="term" value="P:male meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of vascular associated smooth muscle cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Forkhead box protein J3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FH_FOXJ2"/>
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        <property type="entry name" value="Fork_head_dom"/>
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    <dbReference type="InterPro" id="IPR045912">
        <property type="entry name" value="FOXJ2/3-like"/>
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    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="FORKHEAD BOX PROTEIN J2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46078">
        <property type="entry name" value="FORKHEAD BOX PROTEIN J2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORKHEAD"/>
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        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="66"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="66"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="125"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="523"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="561" mass="60824" checksum="286B5520A0374A47" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GF89</accession>
    <name>A0A4W2GF89_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">26S proteasome regulatory subunit p27</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PSMD9</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with PSMC3. Part of a transient complex (modulator) containing PSMD9, PSMC6 and PSMC3 formed during the assembly of the 26S proteasome.</text>
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        <text evidence="1">Belongs to the proteasome subunit p27 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027566867.1"/>
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    <dbReference type="SMR" id="A0A4W2GF89"/>
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        <property type="gene ID" value="ENSBIXG00000015221.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005028079.1">
        <property type="protein sequence ID" value="ENSBIXP00005016648.1"/>
        <property type="gene ID" value="ENSBIXG00005020244.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PSMD9"/>
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    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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            <end position="194"/>
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            <end position="127"/>
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        <location>
            <begin position="1"/>
            <end position="11"/>
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            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054581"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062195"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="221" mass="24266" checksum="42C4BE7D200453BC" modified="2019-09-18" version="1">MSEKAGSQSGGSPEASGVTVSDVQELIRRKEEIEAQIKANYEVLESQKGIGMNEPLVDCEGYPRADVDLYQVRTARHNIVCLQNDHKAVMKQVEDALHQLHARDKEKQARDLAEAHREALSRDQSQGLSPAQAFAKVNSISPGSPASIAGLQVDDEILEFGSVNTQNFQSLQNIGSVVQHSEGKPLNVTVMRRGEKHQLRLVPTRWAGKGLLGCNIIPLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GUU1</accession>
    <name>A0A4W2GUU1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cerebral dopamine neurotrophic factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CDNF</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARMET family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027415688.1">
        <property type="nucleotide sequence ID" value="XM_027559887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GUU1"/>
    <dbReference type="SMR" id="A0A4W2GUU1"/>
    <dbReference type="STRING" id="30522.A0A4W2GUU1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022661.1">
        <property type="protein sequence ID" value="ENSBIXP00000034648.1"/>
        <property type="gene ID" value="ENSBIXG00000017699.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036817.1">
        <property type="protein sequence ID" value="ENSBIXP00005022457.1"/>
        <property type="gene ID" value="ENSBIXG00005025344.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903557"/>
    <dbReference type="CTD" id="441549"/>
    <dbReference type="GeneTree" id="ENSGT00390000007160"/>
    <dbReference type="OMA" id="DSWGEVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071542">
        <property type="term" value="P:dopaminergic neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000003">
        <property type="entry name" value="Mesencephalic astrocyte-derived neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000003">
        <property type="entry name" value="Mesencephalic astrocyte-derived neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045333">
        <property type="entry name" value="ARMET-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019345">
        <property type="entry name" value="ARMET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045332">
        <property type="entry name" value="ARMET_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12990">
        <property type="entry name" value="ARMET-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12990:SF9">
        <property type="entry name" value="CEREBRAL DOPAMINE NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10208">
        <property type="entry name" value="ARMET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20145">
        <property type="entry name" value="ARMET_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611764" evidence="6">
        <location>
            <begin position="27"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="ARMET N-terminal" evidence="8">
        <location>
            <begin position="36"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="ARMET C-terminal" evidence="7">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="187" mass="20732" checksum="AD6857903470B509" modified="2019-09-18" version="1">MWCGGAAAMVAFCAGLWVSNPGRAQGQEAGGIPGADCEVCQEFLSRFYNSLIARGVNFSLDTIEKELINFCLDVKGKENRLCYYLGATKDAATKILSEVTRPMSVHMPAAKICEKLKKMDSQICELKYEKKLDLASVDLSKMRVAELKQILHSWGEECRACAEKTDYVNLIKELAPKYAATHPQTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H614</accession>
    <name>A0A4W2H614_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional enhancer factor TEF-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">TEA domain family member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TEAD2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with YAP1 and WWTR1/TAZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027370966.1">
        <property type="nucleotide sequence ID" value="XM_027515165.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027370967.1">
        <property type="nucleotide sequence ID" value="XM_027515166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H614"/>
    <dbReference type="SMR" id="A0A4W2H614"/>
    <dbReference type="STRING" id="30522.A0A4W2H614"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023436.1">
        <property type="protein sequence ID" value="ENSBIXP00000035005.1"/>
        <property type="gene ID" value="ENSBIXG00000018134.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040711.1">
        <property type="protein sequence ID" value="ENSBIXP00005025148.1"/>
        <property type="gene ID" value="ENSBIXG00005027736.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876206"/>
    <dbReference type="CTD" id="8463"/>
    <dbReference type="GeneTree" id="ENSGT00950000182956"/>
    <dbReference type="OMA" id="IFQFWSG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140552">
        <property type="term" value="C:TEAD-YAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001223">
        <property type="term" value="F:transcription coactivator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
        <property type="term" value="P:cellular response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003143">
        <property type="term" value="P:embryonic heart tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048368">
        <property type="term" value="P:lateral mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048339">
        <property type="term" value="P:paraxial mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000736">
        <property type="term" value="P:regulation of stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000002">
        <property type="entry name" value="transcriptional enhancer factor TEF-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="14">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="12">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="187"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="451" mass="49674" checksum="B0F0EF6A3A28F515" modified="2019-09-18" version="1">MGEPRAGAPLDDGSGWTGSEEGSEEGTGGSEGAGGDGGPDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSREIQSKLKALNVDQVSKDKAFQTMATMSSAQLISAPSLQAKLGPAGPQTPELFQFWSGSSGPPWNVPDVKPFSQTPFSLSLTPPSTDLPGYEPPQALSPPALPPPAPSPPAWQARALGTARLQLVEFSAFVEPPDATDSYQRHLFVHISQHCPSPGAPPLESVDVRQIYDKFPEKKGGLRELYDRGPPHAFFLVKFWADLNWGPSGEEVGAGGSSGGFYGVSSQYESLEHMTLTCSSKVCSFGKQVVEKVETERAQLEDGRFVYRLLRSPMCEYLVNFLHKLRQLPERYMMNSVLENFTILQVVTNRDTQELLLCTAYVFEVSTSERGAQHHIYRLVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H756</accession>
    <name>A0A4W2H756_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Calpain 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CAPN8</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H756"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028802.1">
        <property type="protein sequence ID" value="ENSBIXP00000016728.1"/>
        <property type="gene ID" value="ENSBIXG00000020822.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043456.1">
        <property type="protein sequence ID" value="ENSBIXP00005026950.1"/>
        <property type="gene ID" value="ENSBIXG00005005137.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160090"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF374">
        <property type="entry name" value="CALPAIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="45"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 7">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 7">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 7">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="381" mass="42485" checksum="FF20CF3FB9E0ACB6" modified="2019-09-18" version="1">MAALAARVARQRAATEGLGSSQKAVRYLGQDFETLRQQCLDSGLLFKDPEFPACPSALGYKDLGPCSPRTQGVVWKRPMELCPSPQFIVGGATRTDICQGALGDCWLLAAIASLTLNRELLYRVVPRDQSFQENYAGIFRFQFWQYGEWVEVVVDDRLPTKNGKLLFLHSEEGSEFWSALLEKAYAKLNGSYEALTGGSTVEGFEDFTGGISEFYNLKKPPASLFQIIQKALRSGSLLACSIDISSAAETEAITSQKLVKGHAYSVTGVEEVDFRGCPERLIRLRNPWGEVEWKGAWSDDAPEWNYIDPRQKEQLGKKAEDGEFWMSFSDFLRQFSRLEICNLSLDSLSSEEAHKWSLVLFNGRWTRGSTAGGCQNYPGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H926</accession>
    <name>A0A4W2H926_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomal protein of 19 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CEP19</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027393183.1">
        <property type="nucleotide sequence ID" value="XM_027537382.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393192.1">
        <property type="nucleotide sequence ID" value="XM_027537391.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393200.1">
        <property type="nucleotide sequence ID" value="XM_027537399.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393211.1">
        <property type="nucleotide sequence ID" value="XM_027537410.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393220.1">
        <property type="nucleotide sequence ID" value="XM_027537419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H926"/>
    <dbReference type="SMR" id="A0A4W2H926"/>
    <dbReference type="STRING" id="30522.A0A4W2H926"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032806.1">
        <property type="protein sequence ID" value="ENSBIXP00000019283.1"/>
        <property type="gene ID" value="ENSBIXG00000004492.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043134.1">
        <property type="protein sequence ID" value="ENSBIXP00005026710.1"/>
        <property type="gene ID" value="ENSBIXG00005002172.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889942"/>
    <dbReference type="CTD" id="84984"/>
    <dbReference type="GeneTree" id="ENSGT00390000016356"/>
    <dbReference type="OMA" id="AKKCGIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097712">
        <property type="term" value="P:vesicle targeting, trans-Golgi to periciliary membrane compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029412">
        <property type="entry name" value="CEP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19K CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14933">
        <property type="entry name" value="CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="163" mass="19189" checksum="F12BA1DA7380BDC9" modified="2019-09-18" version="1">MMCTAKKCGIRFQPPAIILIYENEIKGKSRQRIMPVRNFSKYSDCSRAAEQLKNNPRHKGYLEQVSLKQLEKLFSFLRGNLWGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKKKDDPNFVYDIEVEFPQDEQLQSCGWDTESAEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HHF4</accession>
    <name>A0A4W2HHF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MARS1</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Plays a role in the synthesis of ribosomal RNA in the nucleolus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2HHF4"/>
    <dbReference type="SMR" id="A0A4W2HHF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026595.1">
        <property type="protein sequence ID" value="ENSBIXP00000036351.1"/>
        <property type="gene ID" value="ENSBIXG00000019811.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003809.1">
        <property type="protein sequence ID" value="ENSBIXP00005031738.1"/>
        <property type="gene ID" value="ENSBIXG00005012184.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075017"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10307">
        <property type="entry name" value="GST_C_MetRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00939">
        <property type="entry name" value="MetRS_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000009">
        <property type="entry name" value="Methionine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000010">
        <property type="entry name" value="methionine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000026">
        <property type="entry name" value="Methionine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000136">
        <property type="entry name" value="methionine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041598">
        <property type="entry name" value="MARS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18485">
        <property type="entry name" value="GST_N_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00762">
        <property type="entry name" value="WHEP_TRS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12 18" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="GST C-terminal" evidence="19">
        <location>
            <begin position="74"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="20">
        <location>
            <begin position="839"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="898" mass="100633" checksum="255EFA0607BE17BD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HMN9</accession>
    <name>A0A4W2HMN9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Glypican 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GPC2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HMN9"/>
    <dbReference type="SMR" id="A0A4W2HMN9"/>
    <dbReference type="STRING" id="30522.A0A4W2HMN9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053148.1">
        <property type="protein sequence ID" value="ENSBIXP00000000437.1"/>
        <property type="gene ID" value="ENSBIXG00000029829.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001193.1">
        <property type="protein sequence ID" value="ENSBIXP00005032612.1"/>
        <property type="gene ID" value="ENSBIXG00005013178.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244897"/>
    <dbReference type="OMA" id="GFHTQPI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019803">
        <property type="entry name" value="Glypican_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF24">
        <property type="entry name" value="GLYPICAN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01207">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 12" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611914" evidence="14">
        <location>
            <begin position="22"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="519"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="578" mass="63145" checksum="741F10082E3CA92B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HWG2</accession>
    <name>A0A4W2HWG2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="23">STEAP4 metalloreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">STEAP4</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395122.1">
        <property type="nucleotide sequence ID" value="XM_027539321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HWG2"/>
    <dbReference type="STRING" id="30522.A0A4W2HWG2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046743.1">
        <property type="protein sequence ID" value="ENSBIXP00000014882.1"/>
        <property type="gene ID" value="ENSBIXG00000019493.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022313.1">
        <property type="protein sequence ID" value="ENSBIXP00005035722.1"/>
        <property type="gene ID" value="ENSBIXG00005016949.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891366"/>
    <dbReference type="CTD" id="79689"/>
    <dbReference type="GeneTree" id="ENSGT00390000008042"/>
    <dbReference type="OMA" id="GWERNPK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033212">
        <property type="term" value="P:iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070207">
        <property type="term" value="P:protein homotrimerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF5">
        <property type="entry name" value="METALLOREDUCTASE STEAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="22">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="21">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="470" mass="53523" checksum="A4B34F0D54974D3D" modified="2019-09-18" version="1">MEKTSTDASPLTMYSSEKQETVCIFGTGDFGRSLGLKMLQCGYSIVFGSRTPGMSSLLPNDAEVMSYSDAAQKSDIIIITIHREHYDFLTELTEVLKGKILVDVSNNLKINQYPESNAEYLAQLVPGTHVVKAFNTISAWALQSGALDASRQVFICGNDSKAKQRVMDVVRSLGLTPLDKGSLMAANEIENYPLQLFPMWRFPFYLSAVLCVFFFFYCVIREVIYPYVYEKRDRTFRLPISIPNRVFPIAALTLLALVYLPGVIAAILQLYRGTKYRRFPDWLDHWMLCRKQLGLIALGFAFLHVLYTLVIPIRYYVRWTSNNRTITQVITKKENPFSTTNSWLSDSYIALGMLGFFLFVLLGITSLPSVSNMVNWREFRFVQSKLGYLTLILCTAHTLVYGGKRFLNPSNLVWYLPSAYVIALIIPCTVLVIKFILILPCIDKTLMRIRQGWERNPKYSESSLNGKTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYJ4</accession>
    <name>A0A4W2HYJ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serpin B5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Maspin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Peptidase inhibitor 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SERPINB5</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with IRF6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serpin family. Ov-serpin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381860.1">
        <property type="nucleotide sequence ID" value="XM_027526059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HYJ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007193.1">
        <property type="protein sequence ID" value="ENSBIXP00000004739.1"/>
        <property type="gene ID" value="ENSBIXG00000011046.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028142.1">
        <property type="protein sequence ID" value="ENSBIXP00005038244.1"/>
        <property type="gene ID" value="ENSBIXG00005020257.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882875"/>
    <dbReference type="CTD" id="5268"/>
    <dbReference type="GeneTree" id="ENSGT00940000160674"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02057">
        <property type="entry name" value="serpinB5_maspin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000009">
        <property type="entry name" value="serpin B5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000014">
        <property type="entry name" value="Serpin family B member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000240">
        <property type="entry name" value="Serpin_B9/Maspin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033836">
        <property type="entry name" value="SERPINB5_serpin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF55">
        <property type="entry name" value="SERPIN B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00676">
        <property type="entry name" value="MASPIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="12">
        <location>
            <begin position="13"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="375" mass="42234" checksum="1B1A7E6F31AE942D" modified="2019-09-18" version="1">MEALRLANSAFAVDLYKQLCEKEPADNILFSPICLSTSLSLAQVGAKGDTADEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVEKSLNLSTEFISSTKRPYAKEMETVDFKDKLEETKGQINNSIKELTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRINQTDTKPVQMMNMEATFCMGHIDGVNCKIIELPFQNKHLSMLILLPKDVEDGPTGLEQVEKQLNSETLLQWTNPSMMANAKVKLSIPKFKVEKMIDPKASLENLGLKNIFNEDTSDFSGMSETKGVALSNVIHRVSLEITEDGGDSIEVPGSRILQHKDEFNADHPFIYIIRHNKTRNIVFFGKFCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I1X2</accession>
    <name>A0A4W2I1X2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">Serine/threonine-protein kinase SBK1</fullName>
            <ecNumber evidence="2 14">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">SH3 domain-binding kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SBK1</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in signal-transduction pathways related to the control of brain development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2 14"/>
    <dbReference type="RefSeq" id="XP_027383008.1">
        <property type="nucleotide sequence ID" value="XM_027527207.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383010.1">
        <property type="nucleotide sequence ID" value="XM_027527209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I1X2"/>
    <dbReference type="SMR" id="A0A4W2I1X2"/>
    <dbReference type="STRING" id="30522.A0A4W2I1X2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011667.1">
        <property type="protein sequence ID" value="ENSBIXP00000002572.1"/>
        <property type="gene ID" value="ENSBIXG00000009027.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030677.1">
        <property type="protein sequence ID" value="ENSBIXP00005039373.1"/>
        <property type="gene ID" value="ENSBIXG00005007164.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883470"/>
    <dbReference type="CTD" id="388228"/>
    <dbReference type="GeneTree" id="ENSGT00940000154852"/>
    <dbReference type="OMA" id="IFCFIKY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13987">
        <property type="entry name" value="STKc_SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000337">
        <property type="entry name" value="Serine/threonine-protein kinase SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016234">
        <property type="entry name" value="Ser/Thr_kinase_Sbk1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000566">
        <property type="entry name" value="Ser/Thr_PK_Sbk1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="53"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="321"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="418" mass="45571" checksum="0F328EC7AC2243FE" modified="2019-09-18" version="1">MSVGCPEPEPPHSLPCCGPGTAPGLGAGVPLLTEDMQALTLRTVAASDVTKHYELVRELGKGTYGKVDLVAYKGTGTKMALKFVNKSKTKLKNFLREVSITNSLSSSPFIIKVFDVVFETEDCYVFAQEYAPAGDLFDIIPPQVGLPEDTVKRCVQQLGLALDFMHGRQLVHRDIKPENVLLFDRECRRVKLADFGMTRRVGCRVKRVSGTIPYTAPEVCQAGRADGFAVDTGVDVWAFGVLIFCVLTGNFPWEAASGADAFFEEFVRWQRGRLPGLPSQWRRFTEPALRMFQRLLALEPERRGPAKEVFRFLKHELTSELRRRPSHRARKPAGDRPPAAGPLRLEAPGPLKRTVLTESGSGSRPAPPAVGPGPVPVPVPVPVPEPGLAPPGPPGRTDGRPDKSKGQVVLATAIEICV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I5U3</accession>
    <name>A0A4W2I5U3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apolipoprotein A-II</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Apolipoprotein A2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">APOA2</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein A2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383590.1">
        <property type="nucleotide sequence ID" value="XM_027527789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I5U3"/>
    <dbReference type="SMR" id="A0A4W2I5U3"/>
    <dbReference type="STRING" id="30522.A0A4W2I5U3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051913.1">
        <property type="protein sequence ID" value="ENSBIXP00000043042.1"/>
        <property type="gene ID" value="ENSBIXG00000027093.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026663.1">
        <property type="protein sequence ID" value="ENSBIXP00005037582.1"/>
        <property type="gene ID" value="ENSBIXG00005019464.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883787"/>
    <dbReference type="CTD" id="336"/>
    <dbReference type="GeneTree" id="ENSGT00390000003306"/>
    <dbReference type="OMA" id="LTICSFE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034366">
        <property type="term" value="C:spherical high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034190">
        <property type="term" value="F:apolipoprotein receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008035">
        <property type="term" value="F:high-density lipoprotein particle binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070653">
        <property type="term" value="F:high-density lipoprotein particle receptor binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0055102">
        <property type="term" value="F:lipase inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060228">
        <property type="term" value="F:phosphatidylcholine-sterol O-acyltransferase activator activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:receptor ligand activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904646">
        <property type="term" value="P:cellular response to amyloid-beta"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071402">
        <property type="term" value="P:cellular response to lipoprotein particle stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:high-density lipoprotein particle assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034384">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060621">
        <property type="term" value="P:negative regulation of cholesterol import"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002719">
        <property type="term" value="P:negative regulation of cytokine production involved in immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050995">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010903">
        <property type="term" value="P:negative regulation of very-low-density lipoprotein particle remodeling"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:phospholipid efflux"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032757">
        <property type="term" value="P:positive regulation of interleukin-8 production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050996">
        <property type="term" value="P:positive regulation of lipid catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050766">
        <property type="term" value="P:positive regulation of phagocytosis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030300">
        <property type="term" value="P:regulation of intestinal cholesterol absorption"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043691">
        <property type="term" value="P:reverse cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034370">
        <property type="term" value="P:triglyceride-rich lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006801">
        <property type="entry name" value="ApoA-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036172">
        <property type="entry name" value="ApoA-II_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11027">
        <property type="entry name" value="APOLIPOPROTEIN A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11027:SF0">
        <property type="entry name" value="APOLIPOPROTEIN A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04711">
        <property type="entry name" value="ApoA-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82936">
        <property type="entry name" value="Apolipoprotein A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0345">HDL</keyword>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612044" description="Apolipoprotein A-II" evidence="9">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="100" mass="11202" checksum="399C4EB3F0D15FB3" modified="2019-09-18" version="1">MKLLALTVLLLTICGLEGALVRRQAEESNLQSLVSQYFQTVADYGKDLVEKAKGSELQTQAKAYFEKTQEELTPFFKKAGTDLLNFLSSFIDPKKQPATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2INP4</accession>
    <name>A0A4W2INP4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SCAN domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC113879525</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027376900.1">
        <property type="nucleotide sequence ID" value="XM_027521099.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00000041384.1"/>
        <property type="gene ID" value="ENSBIXG00000025641.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044069.1">
        <property type="protein sequence ID" value="ENSBIXP00005045771.1"/>
        <property type="gene ID" value="ENSBIXG00005001567.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879525"/>
    <dbReference type="GeneTree" id="ENSGT00940000164888"/>
    <dbReference type="OMA" id="EGYPEIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF14">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="72"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16535" checksum="571B893BB81E5EF4" modified="2019-09-18" version="1">METEGYPEMQRDVEVQNDEIRSPPNERMAEEPEGILIAPPQVQVPPESLDEDSEDDLETVPRRSPLNPAASRQRFRQFRYEDAAGPRDVLRHLQELAGQWLRPDIHTKEQIVEMLVQEQFQAVLPEELRAWALRCQPGVRITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2INZ9</accession>
    <name>A0A4W2INZ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2INZ9"/>
    <dbReference type="STRING" id="30522.A0A4W2INZ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006771.1">
        <property type="protein sequence ID" value="ENSBIXP00000004942.1"/>
        <property type="gene ID" value="ENSBIXG00000011233.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040732.1">
        <property type="protein sequence ID" value="ENSBIXP00005043909.1"/>
        <property type="gene ID" value="ENSBIXG00005027673.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000187885"/>
    <dbReference type="OMA" id="MISIQEN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="116" mass="12776" checksum="E7DDF31187CF98DF" modified="2019-09-18" version="1">VHGHEESWGGHQDELQRPQTHMGNGEKVVVADTIAARLEGVADEGRLLITPHALGSHHQHHDSEDEDDREPYSANGSRMGSGPWVRACVPIHHRPVLGSHMLLYTQCLHNGTFVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BKP8</accession>
    <name>A0A4W2BKP8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CLDN6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027382799.1">
        <property type="nucleotide sequence ID" value="XM_027526998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BKP8"/>
    <dbReference type="SMR" id="A0A4W2BKP8"/>
    <dbReference type="STRING" id="30522.A0A4W2BKP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053235.1">
        <property type="protein sequence ID" value="ENSBIXP00000000395.1"/>
        <property type="gene ID" value="ENSBIXG00000029878.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013041.1">
        <property type="protein sequence ID" value="ENSBIXP00005028716.1"/>
        <property type="gene ID" value="ENSBIXG00005008448.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883339"/>
    <dbReference type="CTD" id="9074"/>
    <dbReference type="GeneTree" id="ENSGT00940000163060"/>
    <dbReference type="OMA" id="NHYMARY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001618">
        <property type="term" value="F:virus receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003925">
        <property type="entry name" value="Claudin6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01445">
        <property type="entry name" value="CLAUDIN6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="221" mass="23545" checksum="9376E51AFCEC849B" modified="2019-09-18" version="1">MASAGLQILGIVLTLFGWVNALVSCTLPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVITLLVALLGLLVYLAGAKCTTCVEDKDSKARLVLTSGIIFVISGVLTLIPVCWTAHTIIQDFYNPLVVEAQKRELGASLYLGWAASGLLLLGGGLLCCTCPSGGSRSSSHYMARYSVSAPHTASRGPSEYPTKNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BLR6</accession>
    <name>A0A4W2BLR6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BLR6"/>
    <dbReference type="STRING" id="30522.A0A4W2BLR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015331.1">
        <property type="protein sequence ID" value="ENSBIXP00000000831.1"/>
        <property type="gene ID" value="ENSBIXG00000029306.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04029">
        <property type="entry name" value="C2A_SLP-4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037303">
        <property type="entry name" value="SLP-4/5_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF4">
        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="237"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="389" mass="44181" checksum="67B3E6FEBB46390B" modified="2019-09-18" version="1">MATVTIPGRDKREEKQLCHIQGTKRASSFHNATTVKELEGSPTPSRRAHFLYVLALGFYCSMSFEVPKLKSGRSVLEAESESLDSYTADSDTMSRRDSLDKSGLFPEWKKMSAPKPQVEKEAQLRGQNVVSVDEGEMIFKKNTRKILRPSEYTKSVIDLRPEDVGHESGALGDRSKSVPGLSVDMEEEEEEEDIDHLVKLHRQKLARSSMRSGSSMSTIGSMMSIYSEAGDFGNIFVTGKIAFSLKYEQQTQALVIHVKECQQLAYADEAKKRSNPYVKAYLLPDKSRQGKRKTSIKQDTINPLYDEILRYEIPESLLAQRTLQFSVWHHGRFGRNTFLGEAEVQMDSWKLDKKLDHCLPLHGKVFNSNFRCSWMASVSNLFCYKPGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BLX2</accession>
    <name>A0A4W2BLX2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tripartite motif-containing protein 64-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
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        <property type="entry name" value="zf-RING_UBOX"/>
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        <property type="entry name" value="BUTYPHLNCDUF"/>
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        <property type="entry name" value="BBOX"/>
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        <property type="entry name" value="RING"/>
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        <property type="entry name" value="B-box zinc-binding domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="56"/>
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    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="51378" checksum="F6693B208CB579E2" modified="2019-09-18" version="1">MDSDTLQVFQSELTCSVCMNCFLDPITIDCGHSFCRPCLSLCWEEGQTPRRCPECRGIAETPDFKTNIVLKRLVSLARQARADHDHSSEEQICVTHQEAKGFFCEADQTLLCGPCSERPEHTAHSHSPIHRAAEESREKLLKRMGSLWKIREEMQILLNQEAKKTWSFKDYVALRKEMITAEYQRMLLFLREEEQLHLEALEREAKEMCEQLEESVFRMTQYRASLKEMYRELTGMCCKPDRELLQVRKEEMQKPQPVNPELTCLPVTGILDMLNNFRVDNILSQTTTIHHMSLSEDGNDASVMFRDEHHGTSRQPQIVESVVSWGTRAFASGRHYWELDVTHSSSWILGICKDILITNTSINIYSDEACILFSKKVNNHYSLFTNTPPYIQYVKRPLGRIGVFLDYDNGTVSFYDVCRGSLIYRFLPSPFSFPLKPFLCLRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BM42</accession>
    <name>A0A4W2BM42_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">glutamate carboxypeptidase II</fullName>
            <ecNumber evidence="20">3.4.17.21</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BM42"/>
    <dbReference type="SMR" id="A0A4W2BM42"/>
    <dbReference type="STRING" id="30522.A0A4W2BM42"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015111.1">
        <property type="protein sequence ID" value="ENSBIXP00000000945.1"/>
        <property type="gene ID" value="ENSBIXG00000029157.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 29"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000059">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000002">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF36">
        <property type="entry name" value="GLUTAMATE CARBOXYPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="22">
        <location>
            <begin position="171"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="24">
        <location>
            <begin position="357"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="23">
        <location>
            <begin position="627"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000945.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="750" mass="84620" checksum="C4CD414D9B73B3F6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BM72</accession>
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        <recommendedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 4H</fullName>
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        <name evidence="14" type="primary">EIF4H</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Bovinae</taxon>
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Koren S."/>
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                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="228" mass="25219" checksum="5074AC696268B9F5" modified="2019-09-18" version="1">MADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGFRDDFLGGRGGSRPGDRRTGPPMGSRFRDGPPLRGPNMDFREPTEEERAQRPRLQLKPRTVATPLNQVANPNSAIFGGARPREEVVHKEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTB0</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="7" type="primary">XIRP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
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    </feature>
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    </feature>
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        <location>
            <begin position="1055"/>
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        </location>
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        <location>
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            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1412"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1487"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1540"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1579"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1626"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1646"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1659"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1692"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1755"/>
            <end position="1767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1820" mass="194754" checksum="9D4FC23F245AB59A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BU68</accession>
    <name>A0A4W2BU68_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Endothelial lipase</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
            <ecNumber evidence="5">3.1.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phospholipase A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LIPG</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Exerts both phospholipase and triglyceride lipase activities. More active as a phospholipase than a triglyceride lipase. Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1,2,3-tributanoylglycerol + H2O = dibutanoylglycerol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40475"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:35020"/>
            <dbReference type="ChEBI" id="CHEBI:76478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41384"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64563"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18689"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57875"/>
            <dbReference type="EC" id="3.1.1.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 24">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EC" id="3.1.1.32" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027381722.1">
        <property type="nucleotide sequence ID" value="XM_027525921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BU68"/>
    <dbReference type="SMR" id="A0A4W2BU68"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009360.1">
        <property type="protein sequence ID" value="ENSBIXP00000003687.1"/>
        <property type="gene ID" value="ENSBIXG00000010105.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039521.1">
        <property type="protein sequence ID" value="ENSBIXP00005043279.1"/>
        <property type="gene ID" value="ENSBIXG00005004622.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882788"/>
    <dbReference type="CTD" id="9388"/>
    <dbReference type="GeneTree" id="ENSGT00940000159394"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032376">
        <property type="term" value="P:positive regulation of cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010983">
        <property type="term" value="P:positive regulation of high-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050746">
        <property type="term" value="P:regulation of lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043691">
        <property type="term" value="P:reverse cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01758">
        <property type="entry name" value="PLAT_LPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000014">
        <property type="entry name" value="Endothelial lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000109">
        <property type="entry name" value="Lipase, endothelial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002330">
        <property type="entry name" value="Lipo_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF13">
        <property type="entry name" value="ENDOTHELIAL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00822">
        <property type="entry name" value="LIPOLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 25" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610307" description="Endothelial lipase" evidence="25">
        <location>
            <begin position="21"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="26">
        <location>
            <begin position="347"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="21">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="21">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="21">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00074427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="500" mass="56307" checksum="87D64106DB7EB92B" modified="2019-09-18" version="1">MRNTVLQLCLWSAYCGFATGDPTPLGPEGRLQGEFDKSRVVPTAAKPPVRFNLRTSEDPEDEGCDLSLGHSQPLEDCGFNVTAKTFFIIHGWTMSGMFENWLYKLVSALQTREKGANVVVVDWLPLAHQLYVDAVNNTRGVGHSIAQMLDWLQGKEDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPLFEGADVHRRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTIAEVLKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKTRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEMEPTFYVTLYGTNADSQVLSLEMVEQIGLNATNTFLVYTEEDLGDLLKIKLTWEGKSQSWYNLWKELRSYLTQSRGSKQELHIRRIRVKSGETQRRLTFCAEDLENTLISPGQELWFHKCLDGWRMKNQTSPTVEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYT8</accession>
    <name>A0A4W2BYT8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYT8"/>
    <dbReference type="SMR" id="A0A4W2BYT8"/>
    <dbReference type="STRING" id="30522.A0A4W2BYT8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008001.1">
        <property type="protein sequence ID" value="ENSBIXP00000004354.1"/>
        <property type="gene ID" value="ENSBIXG00000007461.1"/>
    </dbReference>
    <dbReference type="OMA" id="RSKYIQP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090734">
        <property type="term" value="C:site of DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16480">
        <property type="entry name" value="RING-H2_TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052639">
        <property type="entry name" value="TRAIP_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="443"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="76"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="194"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="472" mass="53466" checksum="8F9144E687E2357E" modified="2019-09-18" version="1">MPIRALCTICSDFFDHSRDVAAIHCGHTFHSHCLIQWFETAPSRTCPQCRIQVGKRTIINKLYFDLAPEEENVLDTEFLKNELDNTRALLSQKEKEKRDSQLIIDSLRDTLDERNATVESLQRALDKAEMLCSTLKKQMKYLEQQQDEAKQAREEARRLRSKMKNMERIELLLRSQRPEVEEMIRDMGVGQSAVEQLAVYCVSLKKEYENLKEARKASGELADKLKKDLLSSRSKLQTVCSELDQAKLELKSSQKDLQSADKEIASLKKKLTILQETLKLPPGDSETINRLVLESPAPMEMVSLKLRRPAFGDDIDLNATFNVDTPPTQPSSTQYGLAKRLFPKKAHSPVQDIPKKMPKGPRQESQLSLGGQRCLGEPDEELASAFPVFIRNAVLGQKQTKRVRAEPCRSTDAVRIGFDGLGGRTKFIQPTDTTMIRPLPVKPKPKARARQKLGARTAPTPSQATLDNFLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C4C3</accession>
    <name>A0A4W2C4C3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LIPK</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol. Shows a preferential hydrolysis at the sn-3 position of triacylglycerol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39931"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:39932"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1,2,3-trioctanoylglycerol + H2O = 1,2-dioctanoyl-sn-glycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:76978"/>
            <dbReference type="ChEBI" id="CHEBI:76979"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:40048"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384823.1">
        <property type="nucleotide sequence ID" value="XM_027529022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C4C3"/>
    <dbReference type="STRING" id="30522.A0A4W2C4C3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001471.1">
        <property type="protein sequence ID" value="ENSBIXP00000007594.1"/>
        <property type="gene ID" value="ENSBIXG00000013561.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033855.1">
        <property type="protein sequence ID" value="ENSBIXP00005020465.1"/>
        <property type="gene ID" value="ENSBIXG00005006003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884437"/>
    <dbReference type="CTD" id="643414"/>
    <dbReference type="GeneTree" id="ENSGT00940000160031"/>
    <dbReference type="OMA" id="GYPYEKY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000012">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610361" description="Lipase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="13">
        <location>
            <begin position="77"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="396" mass="45437" checksum="171B8CF670DAD1D9" modified="2019-09-18" version="1">MWQLLAATCWMLLLGPVFGYHKKGCTTNPEATMNISQIISYWGYPYEIYDVVTEDGYILGTYRIPHGRGCPKTAPKPVVYLQHGLVASASNWICNLPNNSLAFLLADVGYDVWLGNSRGNTWSRKHLKFSPKSPEYWAFSLDEMAKYDLPATINFIIKKTRQEQLYYVGHSQGTTIAFIAFSTNPELAKRIKIFFALAPVTTLKYTQSPMKKLTNLSRKAVKVLFGDKMFSPHTFFEQFIATKVCNRKIFRRICSNFIFTLSGFDPKNLNMSRLDVYFAQSSAGTSVQTMLHWAQAVNSGRFQAFDWGNPDQNMKHFHQLTPPLYNVSNMEVPTAVWSGGQDYVADLKDVENLLPKIPKLIYYKSIPHYNHVDFYLGQDAPLEIYQDLIRMMEEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6K0</accession>
    <name>A0A4W2C6K0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 3C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPP1R3C</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with PPP1CC catalytic subunit of PP1 and associates with glycogen. Forms complexes with glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to glycogen phosphorylase glycogen synthase and phosphorylase kinase.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384716.1">
        <property type="nucleotide sequence ID" value="XM_027528915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6K0"/>
    <dbReference type="SMR" id="A0A4W2C6K0"/>
    <dbReference type="STRING" id="30522.A0A4W2C6K0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001851.1">
        <property type="protein sequence ID" value="ENSBIXP00000007442.1"/>
        <property type="gene ID" value="ENSBIXG00000013374.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050985.1">
        <property type="protein sequence ID" value="ENSBIXP00005021697.1"/>
        <property type="gene ID" value="ENSBIXG00005024657.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884360"/>
    <dbReference type="CTD" id="5507"/>
    <dbReference type="GeneTree" id="ENSGT00940000155648"/>
    <dbReference type="OMA" id="FCISYQS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22815">
        <property type="entry name" value="PBD_PPP1R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2440:FF:000001">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017434">
        <property type="entry name" value="Pase-1_reg-su_3B/C/D_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030683">
        <property type="entry name" value="PP1_3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF15">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038207">
        <property type="entry name" value="PP1_GT_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500813">
        <property type="entry name" value="PP1_PTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 3" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="4">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR038207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="318" mass="36317" checksum="DCBE545123529554" modified="2019-09-18" version="1">MSCTRMIQVLDPRPLTSSVMPVDVAVRLCLAHSPPLKSFLSPYDDFQRRNFVNKLKPLKSCLNIKQEARAQDDWKPSHNQAKKRVVFADSKGLSLTAIHVFSDLPEEPVWDLQFDLLDLNDISSGLKLHEEKNLILDFPQPSADYLSFRNHFQKNSVCLENCSLQERTVTGTVKVKNMSFEKKVQIRITFDSWQSYNDVDCAYMKNVYGGSESDTFSFAIDLPSVIPTKEKIEFCVAYHANGQVFWDNNEGQNYRIVHVQWKPDGVQTQMAAQDCAFHQAPPPKAELESTVFGSPRLASGLFPEWQSWGRMENLASYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCR3</accession>
    <name>A0A4W2CCR3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 527</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF527</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCR3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044763.1">
        <property type="protein sequence ID" value="ENSBIXP00000010715.1"/>
        <property type="gene ID" value="ENSBIXG00000002283.1"/>
    </dbReference>
    <dbReference type="OMA" id="KEPWMVQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002371">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000006">
        <property type="entry name" value="Zinc finger protein 184 (Kruppel-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000101">
        <property type="entry name" value="zinc finger protein 436 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001174">
        <property type="entry name" value="zinc finger protein 527 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001401">
        <property type="entry name" value="zinc finger protein 527 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001487">
        <property type="entry name" value="zinc finger protein 527 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002050">
        <property type="entry name" value="zinc finger protein 527 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF247">
        <property type="entry name" value="ZINC FINGER PROTEIN 977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="554"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="609" mass="71245" checksum="1A984DF5F4FDE527" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCX5</accession>
    <name>A0A4W2CCX5_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Signal induced proliferation associated 1 like 3</fullName>
        </submittedName>
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        <name evidence="8" type="primary">SIPA1L3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Rap1_Ran_GAP"/>
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    </dbReference>
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        <property type="entry name" value="SIGNAL-INDUCED PROLIFERATION-ASSOCIATED 1-LIKE PROTEIN 3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02145">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </feature>
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        </location>
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        </location>
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        </location>
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        <location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1788" mass="194648" checksum="CED35A5926118F8B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CF22</accession>
    <name>A0A4W2CF22_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLCO2B1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
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        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:monoatomic ion transport"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0043252">
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
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    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 2B1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KAZAL_2"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="483"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="683"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="709" mass="76733" checksum="329ED3A3BEDE6C7F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFV4</accession>
    <name>A0A4W2CFV4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-2-glycoprotein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Apolipoprotein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Beta-2-glycoprotein I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">APOH</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CFV4"/>
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        <property type="gene ID" value="ENSBIXG00000017059.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011871.1"/>
        </source>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="345" mass="38245" checksum="EB292C0CCD894AFE" modified="2019-09-18" version="1">MLPPALVLLLGFLCHVAIAGRTCPKPDELPFSTVVPLKRTYEPGEQIVFSCQPGYVSRGGIRRFTCPLTGLWPINTLKCMPRVCPFAGILENGTVRYTTFEYPNTISFSCHTGFYLKGASSAKCTEEGKWSPDLPVCAPITCPPPPIPKFASLSVYKPLAGNNSFYGSKAVFKCLPHHAMFGNDTVTCTEHGNWTQLPECREVRCPFPSRPDNGFVNHPANPVLYYKDTATFGCHETYSLDGPEEVECSKFGNWSAQPSCKASCKLSIKRATVIYEGERVAIQNKFKNGMLHGQKVSFFCKNKEKKCSYTEDAQCIDGTIEIPKCFKEHSSLAFWKTDASDVKPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHI1</accession>
    <name>A0A4W2CHI1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 Z</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Uba6-specific E2 conjugating enzyme 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin carrier protein Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin-protein ligase Z</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A4W2CHI1"/>
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    <dbReference type="STRING" id="30522.A0A4W2CHI1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005121.1">
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23809">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000046">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF26">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="17">
        <location>
            <begin position="186"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="441" mass="47581" checksum="B940164DD9C75CA0" modified="2019-09-18" version="1">MRAGPVHTLAWRAQTPTLSGGLWVDALAERFSLLWEGRGGASAARAREARVGTLCPRSRWCRSGREQRPLWSADVLPSSPGSEGAMAESPTEEAATATAGAGAAGPGASGVTGVVGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGAALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENTEMDSDSSSSGTETDLHGSLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ24</accession>
    <name>A0A4W2CJ24_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="22">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. In podocytes, is involved in the regulation of lamellipodia formation. Acts downstream of AVIL to allow ARP2/3 complex assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="22"/>
    <dbReference type="STRING" id="30522.A0A4W2CJ24"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004001.1">
        <property type="protein sequence ID" value="ENSBIXP00000006312.1"/>
        <property type="gene ID" value="ENSBIXG00000012482.1"/>
    </dbReference>
    <dbReference type="OMA" id="FPETAWQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16203">
        <property type="entry name" value="EFh_PI-PLCepsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08596">
        <property type="entry name" value="PI-PLCc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17229">
        <property type="entry name" value="RA1_PLC-epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01780">
        <property type="entry name" value="RA2_PLC-epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000092">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000085">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000086">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000118">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000090">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046973">
        <property type="entry name" value="PLC-epsilon1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028398">
        <property type="entry name" value="PLC-epsilon1_RA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046974">
        <property type="entry name" value="PLC_epsilon1_EF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF6">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="13 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 22" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="17 22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="19" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="25">
        <location>
            <begin position="1026"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="24">
        <location>
            <begin position="1122"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="26">
        <location>
            <begin position="1406"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="328"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="954"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1525"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="850"/>
            <end position="862"/>
        </location>
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            <end position="1551"/>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00059831"/>
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        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1572" mass="176691" checksum="1A3F1DD3896F1083" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ98</accession>
    <name>A0A4W2CJ98_BOBOX</name>
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        <submittedName>
            <fullName evidence="7">Reticulon 4 receptor like 2</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">RTN4RL2</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="RETICULON 4 RECEPTOR LIKE 1"/>
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    </dbReference>
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        <property type="entry name" value="L domain-like"/>
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    </dbReference>
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        <location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="461" mass="50013" checksum="FD2FB6BABD52AA30" modified="2019-09-18" version="1">MLCTCYPAPPTVSCQANNFSAVPRALPPGTQRLFLQNNLIGALRPGSFGPSLLTLWLFSNNLSAIYPGTFRHLQALEELDLGDNRHLRSLEPDTFQGLERLQSLHLYRCQLSSLPGTIFRGLVSLQYLYLQENSLVHLQEAIAGPANHPRRKASLSVSGLWISSCDPLPTPTWTALLLPHPGLGPDCPAPCASDADSDSNDSNSNPAFIEHQDDLFADLANLSHLFLHGNRLRLLTEHVFRGLGSLDRLLLHGNRLQGVHRAAFRGLGRLTILYLFNNSLASLPGEALADLPSLEFLRLNANPWACDCRARPLWAWFQRARVSSSDVTCASPPERRGRDLRALREADFQACPPAAPTRPGGRARANSSSNHLYGVAEAGAPPADPSTLYRDLPAEDARGSPGGDAPTEEDYWGGYGSEDRRGEQTCPGAACQAPPDSRAPARAPGLAAPLLCLLILAPHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKG9</accession>
    <name>A0A4W2CKG9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904781</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CKG9"/>
    <dbReference type="STRING" id="30522.A0A4W2CKG9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045568.1">
        <property type="protein sequence ID" value="ENSBIXP00000012436.1"/>
        <property type="gene ID" value="ENSBIXG00000017489.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032787.1">
        <property type="protein sequence ID" value="ENSBIXP00005019770.1"/>
        <property type="gene ID" value="ENSBIXG00005022924.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286908"/>
    <dbReference type="OMA" id="HHYGHSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF87">
        <property type="entry name" value="OLFACTORY RECEPTOR 51F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="48"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="332" mass="36916" checksum="26F4856FFE996352" modified="2019-09-18" version="1">LTPLIMWVLNNTSAQPLTFLLTGIPGLRAAKIWVSIPVCLLYAGALSGNSVILFVVLREQSLHEPMYYFLSMLSATDLSLSLCTLSTTLGVFWFEAREINLNACVAQMFFLHGFTFMESGVLLAMAFDRFVAICDPLRYTTILTNARIAQIGMSMLTRNVAVMLPVVLFVKRLSFCSSMVLSHSYCYHVDLIQLSCTDNRINSILGLFALFSTTGFDCPCILLSYVLIIRSVLSITSIEGRQKAFNTCISHISAVAIFYIPLISLSLVHRYGHSAPPFVHTIMANIFLLIPPVLNPIIYSVKTKQIQKAVIKVLIQKELILLAHALFLTLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPI4</accession>
    <name>A0A4W2CPI4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA fragmentation factor subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA fragmentation factor 45 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Inhibitor of CAD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DFFA</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibitor of the caspase-activated DNase (DFF40).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of DFFA and DFFB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027420746.1">
        <property type="nucleotide sequence ID" value="XM_027564945.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2CPI4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000015073.1"/>
        <property type="gene ID" value="ENSBIXG00000019643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906591"/>
    <dbReference type="CTD" id="1676"/>
    <dbReference type="OMA" id="NWDIRKT"/>
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        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:deoxyribonuclease inhibitor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:apoptotic DNA fragmentation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of apoptotic DNA fragmentation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900118">
        <property type="term" value="P:negative regulation of execution phase of apoptosis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA fragmentation factor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CIDE-N_dom"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA_fragmentation_mid_dom"/>
    </dbReference>
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        <property type="entry name" value="CELL DEATH ACTIVATOR CIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAATION FACTOR SUBUNIT ALPHA"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CIDE-N" evidence="14">
        <location>
            <begin position="17"/>
            <end position="96"/>
        </location>
    </feature>
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        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
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        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
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        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00059184"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066125"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00082309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="329" mass="36432" checksum="7CDAE6A3D6BA240B" modified="2019-09-18" version="1">MEVPGGPAAPEPGEIRALKQCLLRRNKSREQHGVAASCLKELRNKACDILAIDKSLAPVTLVLAEDGTIVDDDDYFLCLPANTKFVVLAGNEKWTHNSDGGTAWITQESFDRDETDSGAGLKWKNVARQLKEDLSSIILLSEEELQMLIDVPCSELAQELSQSHVAVQGLQNTLQQVLDQREEARQSRQLLELYLRALEKEGGILSKQQESRADLGDESDAVDTGIRESFSEVTPASQILLALKEKPAPELSLSSQDLELVAKEDPKALAVALNWDLKKTEAVQQACNQELRLRLQQVQSLRSLRSISARTSSLPGDMENPKRTRRDPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRS7</accession>
    <name>A0A4W2CRS7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tripartite motif containing 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TRIM14</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRS7"/>
    <dbReference type="STRING" id="30522.A0A4W2CRS7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025515.1">
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        <property type="gene ID" value="ENSBIXG00000019225.1"/>
    </dbReference>
    <dbReference type="OMA" id="WWIGAAY"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13738">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tripartite motif containing 14"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044116">
        <property type="entry name" value="SPRY_PRY_TRIM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF11">
        <property type="entry name" value="TRIPARTITE MOTIF CONTAINING 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="311"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="504" mass="56131" checksum="6215EDF343DCADAE" modified="2019-09-18" version="1">MLRPYPPPHPTPPPPAGLRAAPCYPPFPAGTGRAPPQRPAFDSPRASRCCSAAAQKLGGRMAGSAVLKGTPGGLGPAEPEDARGWLCSEHGDRVVELFCRRCRRCVCALCPVLGSHRGHPIGLALDEAARVQKFTQECLKHLATKKQQQVGNITQIEDAAEKLKAHAESSKTWLTGKFAELRLLLEEEEALAKKFIDKNTQLALQAYTEQIKSCGEQIDVMNTLSNRIWTISQETNPVQLLQEYTATEQQIQQQMSLGELCHPVPHSFEPVKSFFKDLVEAMQSLLQTPLDVRLKESRNCQLSGSCVQPGSLLKTSPSPERSLFLKYARTPCLEPDTMHARLRLSADLLTVRCSLLGRLGPTAALRFDALWQVLSRDCFAAGRHYWEVDVQEAGVGWWVGAAYGSLRRHGASDAARLGCNRQSWCLKRYDLEYWAFHDCQRSRLRPRHDPDRLGVFLDYEAGVLAFYDVTGGMSHLHTFRAAFQEPLYPALRLWEGAISISRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSF1</accession>
    <name>A0A4W2CSF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Rabankyrin-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Ankyrin repeat and FYVE domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Ankyrin repeats hooked to a zinc finger motif</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with RAB5A (in GTP-bound form). Interacts with RHOD (independent of GTP-loaded status). Interacts with EHD1. Interacts with VPS26A; the interaction is independent of EHD1 and is indicative for an association with the cargo recognition subcomplex of the retromer complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSF1"/>
    <dbReference type="STRING" id="30522.A0A4W2CSF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043998.1">
        <property type="protein sequence ID" value="ENSBIXP00000009191.1"/>
        <property type="gene ID" value="ENSBIXG00000014904.1"/>
    </dbReference>
    <dbReference type="OMA" id="WGLEQVV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18501">
        <property type="entry name" value="BACK_ANKFY1_Rank5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18303">
        <property type="entry name" value="BTB_POZ_Rank-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15728">
        <property type="entry name" value="FYVE_ANFY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000116">
        <property type="entry name" value="Ankyrin repeat and FYVE domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000142">
        <property type="entry name" value="Ankyrin repeat and FYVE domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000184">
        <property type="entry name" value="Ankyrin repeat and FYVE domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000210">
        <property type="entry name" value="Ankyrin repeat and FYVE domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000104">
        <property type="entry name" value="Ankyrin repeat and FYVE domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000086">
        <property type="entry name" value="Ankyrin repeat and FYVE domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000166">
        <property type="entry name" value="rabankyrin-5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049765">
        <property type="entry name" value="ANFY1_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049764">
        <property type="entry name" value="ANFY1_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049763">
        <property type="entry name" value="ANKFY1_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF191">
        <property type="entry name" value="RABANKYRIN-5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 18" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="21">
        <location>
            <begin position="68"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="322"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="490"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="543"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="622"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="656"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="771"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="804"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="872"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="907"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="940"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="1045"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="22">
        <location>
            <begin position="1106"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="6"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1171" mass="129134" checksum="3B8C14835A1C9A45" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSF2</accession>
    <name>A0A4W2CSF2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="455" mass="52313" checksum="188BF0E3178300AA" modified="2019-09-18" version="1">MPASLENSAVAKDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYMNRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKTLKEMGIPEHLICLLRNVYAGQEATVRTGHGTTDWFQIGKEVRKGYILSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINHLRYADDTTLMAETEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSDFIFGGSKITADGDCSHEVKRRLLLGRKVMTNLDSIFKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAEHQRIDGFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRNVDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVNLSELQELVMDREAWRAVIHGLQRVGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUC0</accession>
    <name>A0A4W2CUC0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <location>
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            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1272" mass="149545" checksum="7AC06FDC00EF9A24" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CX49</accession>
    <name>A0A4W2CX49_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="169" mass="19528" checksum="4D0F2FD7B8228D35" modified="2019-09-18" version="1">DGDGPVRLRAIEGRVKCGIFGSMDSSYIMKDVLPGPYPKTLEEQVTIAKKYNMQVEDEEPWLDDGMGYGNYLKFPDSSQQERDPWCDWDHPDLMLNWGEPRLWGLGVYIRKRMDVSSMPVSWNLTCKQLHSFTAFMTFMFWVWGELYHSPGNTFLFKMLSSSEVGSFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYQ8</accession>
    <name>A0A4W2CYQ8_BOBOX</name>
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        <recommendedName>
            <fullName evidence="23">Aminopeptidase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">ERAP2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 23">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer with ERAP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYQ8"/>
    <dbReference type="STRING" id="30522.A0A4W2CYQ8"/>
    <dbReference type="GlyCosmos" id="A0A4W2CYQ8">
        <property type="glycosylation" value="5 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000031719.1">
        <property type="protein sequence ID" value="ENSBIXP00000018586.1"/>
        <property type="gene ID" value="ENSBIXG00000022403.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000007">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000046">
        <property type="entry name" value="Endoplasmic reticulum aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="32"/>
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            <end position="263"/>
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        <location>
            <position position="370"/>
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        <ligand>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="455"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00063803"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
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        <source>
            <dbReference type="RuleBase" id="RU364040"/>
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        <source>
            <dbReference type="Pfam" id="PF01433"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11838"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17900"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="960" mass="110415" checksum="B439E24EF264828F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D184</accession>
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        <recommendedName>
            <fullName evidence="2">5'-AMP-activated protein kinase subunit gamma-1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="17" type="primary">PRKAG1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 14" id="KW-0129">CBS domain</keyword>
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    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="125"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="198"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="272"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="330" mass="37497" checksum="40C9C8D5B1798FDA" modified="2019-09-18" version="1">MEAVPSSDSYPAVENEHLQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2B3</accession>
    <name>A0A4W2D2B3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2B3"/>
    <dbReference type="STRING" id="30522.A0A4W2D2B3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022926.1">
        <property type="protein sequence ID" value="ENSBIXP00000012801.1"/>
        <property type="gene ID" value="ENSBIXG00000003049.1"/>
    </dbReference>
    <dbReference type="OMA" id="ISTLWFI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15936">
        <property type="entry name" value="7tmA_OR4D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="101"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="371" mass="41703" checksum="85D59E0043A12486" modified="2019-09-18" version="1">MAAVPVCSDFGAKKIKFVTASTFPLLCAMTGRPLEWQTTPGFLPQEPHEWKLRKKKKHDPMEQGNHSRVKEFIFQGLTQSRELSRVLFLLLSLVYTTTVLGNLLIMVTVSCESRLQTPMYFLLRNLSVLDICFSSTTAPKVLVDLLSERKTISFRGCLTQMFFFHLLGGADIFSLSVMAFDRYVAISKPLHYVTIMSRGRCIGLIVASWAGGFVHSIVQISLLLPLPFCGPNIVDGFYCDVPQVLTLACTDTFVLELLMISNNGLISTLWFVFLLVSYTVILMMLRSHSGEGRRKAISTCTAHITVVTLHFVPCIYVYARPFSALPMDRAVSITFTVIIPVLNPLIYTLRNQEMKAAMKRLKKRLGPSEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2S5</accession>
    <name>A0A4W2D2S5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2S5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049248.1">
        <property type="protein sequence ID" value="ENSBIXP00000020107.1"/>
        <property type="gene ID" value="ENSBIXG00000023477.1"/>
    </dbReference>
    <dbReference type="OMA" id="AHMNCSH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF38">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021266135" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="160" mass="17747" checksum="D0B6464708DC2252" modified="2019-09-18" version="1">MLSSLLRLASLCLLGARCISAEVAQSPSHRVIEKKQAVNLSCDPISGHESLFWYYHAVGKEMKLLIYFLRESMQDDSGMPKGRFTAERMEGTSSTLRVHSAELGDSGVYFCASSRDTVLQSSVLSVQNHILSAPLKSGSPEQKSFPMPPLQGREERNNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2U3</accession>
    <name>A0A4W2D2U3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2U3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049268.1">
        <property type="protein sequence ID" value="ENSBIXP00000020147.1"/>
        <property type="gene ID" value="ENSBIXG00000004663.1"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="RT_dom"/>
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    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="410" mass="47316" checksum="4102B4D1039911B3" modified="2019-09-18" version="1">MLKILQARLRQYVNRELPDVQAGFRKGRGIRNQIANIRWIIEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNINNVRYADDTTLMAESKEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITLWEINGETVSDFLFWGSKITADGDCSHEIKRCLLLGRKVMSNLDSIFKSRDITLPTKVRLVKAMVFPVIMYGCESWTVKKAERQRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRRGRQRMRWLDGITDSMDVSLSELWELVMDREGWRAVILGVAGSRTRLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D580</accession>
    <name>A0A4W2D580_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D580"/>
    <dbReference type="STRING" id="30522.A0A4W2D580"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048937.1">
        <property type="protein sequence ID" value="ENSBIXP00000019407.1"/>
        <property type="gene ID" value="ENSBIXG00000023028.1"/>
    </dbReference>
    <dbReference type="OMA" id="GTHFCAV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042605">
        <property type="term" value="F:peptide antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04983">
        <property type="entry name" value="IgV_TCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051006">
        <property type="entry name" value="TCR_variable_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343:SF24">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 29_DELTA VARIABLE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021458075" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="29"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="153" mass="16976" checksum="B48E63ECF3DE6C3E" modified="2019-09-18" version="1">MSKLLGASLLILWLQSDWVKSQQKNSAQQQVKQNPPSLSVTEGGISILNCDYDNTVLDYFQWYRKYPTKSLAFLISISSVLEKNEDGRFAVFHNRSAKHLSLHISASQPGDSALYLCAASTRCSPGTCSLYSNVLWGLRLRGTHTCFPLCTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5N0</accession>
    <name>A0A4W2D5N0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5N0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035721.1">
        <property type="protein sequence ID" value="ENSBIXP00000021191.1"/>
        <property type="gene ID" value="ENSBIXG00000004906.1"/>
    </dbReference>
    <dbReference type="OMA" id="QYMNHEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="315" mass="35932" checksum="B4E2484F818B5727" modified="2019-09-18" version="1">MVLNFLNFCLSEKLFISPSILNEILAGYSHFGCRFFPFSTLNISCHSLLPCRVSAERSAVKHMDFPLYVTCCFSLDAFNILSLCLIFISLISMCLGVFLLGFILYETLYKKKGSAKECSNYCTIALISHASKVMLKILQARLQQYMNHEFPDVQAGFQKGRGTRDQIANIRWIMDKAREFQKNIYFCFIDYAKAFDCVDHKKLWKILKEMGIPDHLMCLLRNLYSGQEATVRTGHGTTDWFQIGKGVHQGCILSPCLFNLHAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5Z9</accession>
    <name>A0A4W2D5Z9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Max dimerization protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Max-associated protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MXD3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027402684.1">
        <property type="nucleotide sequence ID" value="XM_027546883.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027402685.1">
        <property type="nucleotide sequence ID" value="XM_027546884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5Z9"/>
    <dbReference type="SMR" id="A0A4W2D5Z9"/>
    <dbReference type="STRING" id="30522.A0A4W2D5Z9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035908.1">
        <property type="protein sequence ID" value="ENSBIXP00000021304.1"/>
        <property type="gene ID" value="ENSBIXG00000003809.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035874.1">
        <property type="protein sequence ID" value="ENSBIXP00005041663.1"/>
        <property type="gene ID" value="ENSBIXG00005005767.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895546"/>
    <dbReference type="CTD" id="83463"/>
    <dbReference type="GeneTree" id="ENSGT00940000161050"/>
    <dbReference type="OMA" id="HTDADHC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000073">
        <property type="entry name" value="MAX dimerization protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF6">
        <property type="entry name" value="MAX DIMERIZATION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="57"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="206" mass="23558" checksum="55F7F62E6CCDA9B2" modified="2019-09-18" version="1">MEPVASNIQVLLQAAEFLERREREAEHGYASLCPHRSPGPVHRRRKRSPQAPGALDSGRSVHNELEKRRRAQLKRCLEQLKQQMPLGADCVRYTTLSLLRRARMHIQKLEEQEQRARQLKEKLRSKQQSLRRQLEQLRGLGTVGERERLRADSLDSSGLSSERSDSDQEELEVDVESLVFGGEAELLRGFSAGQEHSYSHSGSAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9J2</accession>
    <name>A0A4W2D9J2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ankyrin repeat and SOCS box containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ASB4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395920.1">
        <property type="nucleotide sequence ID" value="XM_027540119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9J2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037850.1">
        <property type="protein sequence ID" value="ENSBIXP00000022636.1"/>
        <property type="gene ID" value="ENSBIXG00000025429.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045630.1">
        <property type="protein sequence ID" value="ENSBIXP00005011339.1"/>
        <property type="gene ID" value="ENSBIXG00005015829.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891715"/>
    <dbReference type="CTD" id="51666"/>
    <dbReference type="GeneTree" id="ENSGT00940000158874"/>
    <dbReference type="OMA" id="PIQYVLK"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000193">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000271">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF10">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 29-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="426" mass="48324" checksum="32DB2E789DB5EDC5" modified="2019-09-18" version="1">MDGTAAPITKSAAAKLVKRNFLEALKSNDFGKLKAILIQRQIDVDTVFEVEDENMVLASYKQGYWLPSYKLKSSWATGLHLSVLFGHVECLLVLLDHNATINCRPNGKTPLHVACEMANLDCVKILCDRGAKLNCYSLSGHTALHFCTTPNSILCAKQLVWRGANVNMKTNNQDEETPLHTAAHFGLPELVAFYVEHGAIVDSVNAHMETPLAIATYWALRFKEQEYSRDHHLICRMLLDYKAEVNARDDDFKSPLHKAAWNCDHVLMDMMLEAGAEANLMDINGCAAIQYVLKVTSVRPAARPEICYQLLLNHGAARIYPPQFHKVIQACHSYPKAIEVVVNAYEHIKWNVKWKRAIPDDDLERHWDFYHSLFTVCSNSPRTLMHLSRCAIRRTLHSRCHRVIPLLSLPLSLKKYLLLEPEGIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAV1</accession>
    <name>A0A4W2DAV1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CCDC24</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027393298.1">
        <property type="nucleotide sequence ID" value="XM_027537497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAV1"/>
    <dbReference type="STRING" id="30522.A0A4W2DAV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038643.1">
        <property type="protein sequence ID" value="ENSBIXP00000023180.1"/>
        <property type="gene ID" value="ENSBIXG00000005265.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010198.1">
        <property type="protein sequence ID" value="ENSBIXP00005003498.1"/>
        <property type="gene ID" value="ENSBIXG00005009630.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889998"/>
    <dbReference type="CTD" id="149473"/>
    <dbReference type="GeneTree" id="ENSGT00390000011926"/>
    <dbReference type="OMA" id="LASTCRW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031367">
        <property type="entry name" value="CCDC24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28601">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28601:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15669">
        <property type="entry name" value="CCDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="312" mass="34733" checksum="D8D7B22B7A5D64AD" modified="2019-09-18" version="1">MPGDSPSLWELVEEHVPLPERPEVKRILGETTVDLSLELRAEVVMLRSLLQEARSSQAPGSRPTSDLSSLLAPPPLVRDLVRQELRQLLQGLRRKAICEGRDQTQAWVQYSPRVLRFALEEPRRDLPEQEMFQMRAGDPSSCYRDLSVIKDQLNMSHIDQVAGHLRALLEEECRALEREIPILQRCLEEEYIGAARPSKPSLEPTLAELKEQKAAMQQELQSPLRPSCVSSSHRPQPLGSSSQGPGPLSCLHGAAGVWARPLQCCLPAPPLEHCPKPRSLASTCRWGRKIQCSPRKRPASTPMSSAAPQAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBK2</accession>
    <name>A0A4W2DBK2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxynitrite isomerase THAP4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">THAP domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">THAP4</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Heme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo, possibly modulating the transcriptional activity residing in the N-terminal region.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>peroxynitrite = nitrate</text>
            <dbReference type="Rhea" id="RHEA:63116"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:25941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:63117"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the C-terminal section; belongs to the nitrobindin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBK2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050078.1">
        <property type="protein sequence ID" value="ENSBIXP00000021646.1"/>
        <property type="gene ID" value="ENSBIXG00000024688.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07828">
        <property type="entry name" value="lipocalin_heme-bd-THAP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000014">
        <property type="entry name" value="THAP domain-containing protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045165">
        <property type="entry name" value="Nitrobindin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014878">
        <property type="entry name" value="THAP4-like_heme-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15854:SF4">
        <property type="entry name" value="PEROXYNITRITE ISOMERASE THAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15854">
        <property type="entry name" value="THAP4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08768">
        <property type="entry name" value="THAP4_heme-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="24">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="84"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="584" mass="63395" checksum="2144E2F27FBDA5D9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCQ7</accession>
    <name>A0A4W2DCQ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCQ7"/>
    <dbReference type="STRING" id="30522.A0A4W2DCQ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047514.1">
        <property type="protein sequence ID" value="ENSBIXP00000016529.1"/>
        <property type="gene ID" value="ENSBIXG00000003832.1"/>
    </dbReference>
    <dbReference type="OMA" id="YICIFFT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
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        <location>
            <begin position="273"/>
            <end position="292"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
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        <source>
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        </source>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="313" mass="34810" checksum="3045386B9629B554" modified="2019-09-18" version="1">MTRENQSSVSEFLLLGLPIRPEQQGVFFALFLGMYLTTVLGNLLILLLIRLDACLHTPMYFFLSHLALTDVSFSSVTIPKMLINMHTWEQSIPYAGCVTQMYFFIFFTDLENFLLSSMAYDRYVAVCHPLHYSTVMGQGLCTLLVTVSWILSCANALCHTLLLTRLSFCVDLSIPHFFCDLGALLKLSCSDTSLNELAIFTAGVAVIVLPLICILISYGCIGVAILKVPSTKGICKALSTCGSHLSVVSLYYGTIIALYFFPSSNTSRDKSTIASVMYTVVTPLLNPFIYSLRNRDMKEALERLLHRAKVLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DF56</accession>
    <name>A0A4W2DF56_BOBOX</name>
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        <recommendedName>
            <fullName evidence="17">Interleukin-18 receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CD218 antigen-like family member A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">IL1 receptor-related protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Interleukin-18 receptor alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">IL18R1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Within the IL18 receptor complex, responsible for the binding of the pro-inflammatory cytokine IL18, but not IL1A nor IL1B. Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells. Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Forms a ternary complex with IL18 and IL18RAP. Within this complex, IL18R1 is involved in ligand-binding and IL18RAP in signaling leading to NF-kappa-B and JNK activation. Interacts with SLC12A3 in peritoneal macrophages; this interaction is increased by IL18 treatment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410573.1">
        <property type="nucleotide sequence ID" value="XM_027554772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DF56"/>
    <dbReference type="STRING" id="30522.A0A4W2DF56"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029925.1">
        <property type="protein sequence ID" value="ENSBIXP00000017429.1"/>
        <property type="gene ID" value="ENSBIXG00000021434.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003196.1">
        <property type="protein sequence ID" value="ENSBIXP00005007034.1"/>
        <property type="gene ID" value="ENSBIXG00005012403.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900980"/>
    <dbReference type="CTD" id="8809"/>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="OMA" id="HFMGRDE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045092">
        <property type="term" value="C:interleukin-18 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:interleukin-18 binding"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:natural killer cell activation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0120163">
        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of non-canonical NF-kappaB signal transduction"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of T-helper 1 cell cytokine production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of type II interferon production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:T-helper 1 cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Interleukin 1 receptor accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interleukin 18 receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interleukin 18 receptor 1"/>
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        <property type="entry name" value="Interleukin-18 receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF6">
        <property type="entry name" value="INTERLEUKIN-18 RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="217"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="22">
        <location>
            <begin position="370"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="538" mass="61965" checksum="4BECB4EC2B0277EF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGP1</accession>
    <name>A0A4W2DGP1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113899613</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGP1"/>
    <dbReference type="STRING" id="30522.A0A4W2DGP1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048248.1">
        <property type="protein sequence ID" value="ENSBIXP00000017994.1"/>
        <property type="gene ID" value="ENSBIXG00000021895.1"/>
    </dbReference>
    <dbReference type="OMA" id="LRKWDVH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15226">
        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000012">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="55"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="326" mass="36773" checksum="F92A3496AC3CD203" modified="2019-09-18" version="1">MKEVTTEDISWNESMSETNHSTVTEFIFLGLSNSQELQIFLFVFFFVFYVGIVFGNLLIVITVASDSHLHSPMYFLLANLSLIDLCLSSVTAPKTIADFFSKCKVISFKGCLAQIFLLHFFGGSELMILIAMAFDRYVAICKPLHYTTIMRGNVCVGFVAAAWGTGFLHSVSQLAFAVNLPFCGPNEVDSFYCDLPRVVKLACTDTYRLDIMVIVNSGVLTVCSFFLLITSYTVILVTIQHLPSERSSKALSTLTAHITVVLLFFGPCIFIYAWPFPIKSLDKFLAVFYSVVTPFLNPMIYTLRNKDMKTAMRWLRKWNINSRIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DHK2</accession>
    <name>A0A4W2DHK2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Choline transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Choline/H+ antiporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DHK2"/>
    <dbReference type="STRING" id="30522.A0A4W2DHK2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042569.1">
        <property type="protein sequence ID" value="ENSBIXP00000025749.1"/>
        <property type="gene ID" value="ENSBIXG00000027862.1"/>
    </dbReference>
    <dbReference type="OMA" id="KEEFYYG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF42">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="462"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="657"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="721" mass="82405" checksum="A648B35E6B08BCFC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DHZ5</accession>
    <name>A0A4W2DHZ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DHFR</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027401512.1">
        <property type="nucleotide sequence ID" value="XM_027545711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DHZ5"/>
    <dbReference type="SMR" id="A0A4W2DHZ5"/>
    <dbReference type="STRING" id="30522.A0A4W2DHZ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042703.1">
        <property type="protein sequence ID" value="ENSBIXP00000025827.1"/>
        <property type="gene ID" value="ENSBIXG00000027925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052639.1">
        <property type="protein sequence ID" value="ENSBIXP00005024794.1"/>
        <property type="gene ID" value="ENSBIXG00005027356.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895049"/>
    <dbReference type="CTD" id="1719"/>
    <dbReference type="GeneTree" id="ENSGT00390000010283"/>
    <dbReference type="OMA" id="RDNQLPW"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025827.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="187" mass="21603" checksum="56E55F8D742ED894" modified="2019-09-18" version="1">MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFQYFQRMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDVVWIVGGSSVYKEAMNKPGHVRLFVTRIMQEFESDAFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DI41</accession>
    <name>A0A4W2DI41_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Rho guanine nucleotide exchange factor 19</fullName>
        </submittedName>
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        <name evidence="8" type="primary">ARHGEF19</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
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    </dbReference>
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    <feature type="domain" description="DH" evidence="7">
        <location>
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            <end position="565"/>
        </location>
    </feature>
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        <location>
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            <end position="21"/>
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    </feature>
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        <location>
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            <end position="347"/>
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            <end position="320"/>
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        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="807" mass="89078" checksum="5B411AF5DE95311F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIJ6</accession>
    <name>A0A4W2DIJ6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Melanocortin 2 receptor accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MRAP</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MRAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027394155.1">
        <property type="nucleotide sequence ID" value="XM_027538354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIJ6"/>
    <dbReference type="STRING" id="30522.A0A4W2DIJ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040040.1">
        <property type="protein sequence ID" value="ENSBIXP00000024118.1"/>
        <property type="gene ID" value="ENSBIXG00000005505.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890454"/>
    <dbReference type="CTD" id="56246"/>
    <dbReference type="OMA" id="PLCIWRQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031780">
        <property type="term" value="F:corticotropin hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030545">
        <property type="term" value="F:signaling receptor regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070996">
        <property type="term" value="F:type 1 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031781">
        <property type="term" value="F:type 3 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031782">
        <property type="term" value="F:type 4 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031783">
        <property type="term" value="F:type 5 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106070">
        <property type="term" value="P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028111">
        <property type="entry name" value="MRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675:SF2">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15183">
        <property type="entry name" value="MRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="194" mass="21132" checksum="7F6A96D691AC79F3" modified="2019-09-18" version="1">MSGPPPDLPPSPTALISEEALSSETHRHTWMILHKSAGPSPGELGAAPEGPPGSDAGMANRTNISAPYYSYEYYLDYLDLMPVDEKKLRANKHSIVIAFWVSLAAFVVFLFLILLYMSWSGSPQTRNATQQPPTCSWNLGLNLPLCIWRQPSTPGPHRARSRNQTPEHPGCPAATTREPLNLSTPGTILLSSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIV0</accession>
    <name>A0A4W2DIV0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039949.1">
        <property type="protein sequence ID" value="ENSBIXP00000024056.1"/>
        <property type="gene ID" value="ENSBIXG00000026340.1"/>
    </dbReference>
    <dbReference type="OMA" id="QPAQGCY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="135" mass="14041" checksum="03314E29B3E056EC" modified="2025-10-08" version="2">MGFTGPHPPSLGGAEHRSAVYSSSVSTNRLDTAQMAMIRYTFTCTRTASASEAPDNAREEALGAWPLSSSDSSTVAVAVSAGSPWSFTSTSRRWRGASASSRARVVLISPVYSPTRNGVLAPPAAGCSSYDSHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJF0</accession>
    <name>A0A4W2DJF0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">KLF transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KLF2</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJF0"/>
    <dbReference type="STRING" id="30522.A0A4W2DJF0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040580.1">
        <property type="protein sequence ID" value="ENSBIXP00000024427.1"/>
        <property type="gene ID" value="ENSBIXG00000026590.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021017.1">
        <property type="protein sequence ID" value="ENSBIXP00005011900.1"/>
        <property type="gene ID" value="ENSBIXG00005016244.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163163"/>
    <dbReference type="OMA" id="DCHTQMM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000237">
        <property type="entry name" value="Krueppel-like factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF109">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="483"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="513"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="543"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="174"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="330"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="376"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="566" mass="59386" checksum="4FB12D097319B7FA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKM3</accession>
    <name>A0A4W2DKM3_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Myozenin 3</fullName>
        </submittedName>
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        <name evidence="3" type="primary">MYOZ3</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myozenin family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2DKM3"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:telethonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008438">
        <property type="entry name" value="MYOZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941">
        <property type="entry name" value="MYOZENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941:SF15">
        <property type="entry name" value="MYOZENIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05556">
        <property type="entry name" value="Calsarcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="245" mass="27008" checksum="F02E3185094273A8" modified="2019-09-18" version="1">MIPKEQKGPVMTTTGDLTEPVPLLDLGKKLSVPQDLMMEELSLRNNRGSLLFQKRQRRVQKFTFEFAASPRAVVAGSAKEKVTGTAEPGMVANSHEGKNYRSELHIYPASHGGPGDAESMASRLVSTYNPSALAPGYAEPLKGIPPEKFNHTAIPKGYRCPWQEFISYRDYLGEGGSHTPSLVEYRNFNKTPMPFGGPLVGETLPRAGTPFVPELTSGLELLRLRPSFNRVAQGWVRNLPESEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DL08</accession>
    <name>A0A4W2DL08_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DL08"/>
    <dbReference type="STRING" id="30522.A0A4W2DL08"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014464.1">
        <property type="protein sequence ID" value="ENSBIXP00000027112.1"/>
        <property type="gene ID" value="ENSBIXG00000028782.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:virion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.180">
        <property type="entry name" value="Gamma-retroviral matrix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.375.10">
        <property type="entry name" value="Human Immunodeficiency Virus Type 1 Capsid Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_retro_matrix_sf"/>
    </dbReference>
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        <property type="entry name" value="Gag_P30"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050462">
        <property type="entry name" value="Retroviral_Gag-Pol_poly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010999">
        <property type="entry name" value="Retrovr_matrix"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33166">
        <property type="entry name" value="GAG_P30 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02093">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47836">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus capsid protein, N-terminal core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="530" mass="60751" checksum="000A3F63D3E19EAA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLW9</accession>
    <name>A0A4W2DLW9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C12H13orf42</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLW9"/>
    <dbReference type="STRING" id="30522.A0A4W2DLW9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013386.1">
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        <property type="gene ID" value="ENSBIXG00000008250.1"/>
    </dbReference>
    <dbReference type="OMA" id="YSRTQDC"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
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        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="318" mass="36089" checksum="066A8FBC689D7199" modified="2019-09-18" version="1">MLRKLHSILHSGPHRKAEVAESPYYHGPSSAVRLIRSSSVYVVGDHGEKFSESLKKYRSAGSMDPSLYYLQQEGDRAWMLSRTQDCLQYLQELLALRKKYLSSLNDLKPSRAPGVSSTSSKSSRGVKKSAAKEMKKPTPKKYSQFSADVAEAIAFFDSIIAELDTEKRPRAAEVDLQNEDVDFDVATSSREHSLHSNWILRAPRRRSEDVAGHTTAGQFRRSTERRTIGTQRRLERHPIYLPKAVEGAFSTLKFKPKACKKDLGSSRQILFNFSGEDTDWDSELFAPEPPASLGEDHYETENPGQWLLREQLWERTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRG1</accession>
    <name>A0A4W2DRG1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRG1"/>
    <dbReference type="STRING" id="30522.A0A4W2DRG1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036353.1">
        <property type="protein sequence ID" value="ENSBIXP00000021592.1"/>
        <property type="gene ID" value="ENSBIXG00000007648.1"/>
    </dbReference>
    <dbReference type="OMA" id="RTISRWM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4 15" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13 15" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="17">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="140" mass="15659" checksum="E64A80475773E69F" modified="2019-09-18" version="1">MPDPAKSAPAPKKGSKKAVTKVQKKDGKRRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKYTHKYILKSAYTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRQ1</accession>
    <name>A0A4W2DRQ1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rabphilin 3A like (without C2 domains)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPH3AL</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRQ1"/>
    <dbReference type="STRING" id="30522.A0A4W2DRQ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015191.1">
        <property type="protein sequence ID" value="ENSBIXP00000026886.1"/>
        <property type="gene ID" value="ENSBIXG00000029227.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF4">
        <property type="entry name" value="RAB EFFECTOR NOC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RabBD" evidence="4">
        <location>
            <begin position="41"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="374" mass="40876" checksum="36C5AB0E684FE5AE" modified="2019-09-18" version="1">MADTIFGSGCDQWVCPNDRQLALRAKLHTGWSVHTYQTEKQRKSQSLSPAEVEAILQVIQRAERLDILEQQRVGRLVERLETMRRNVMGNGLSQCLLCGEVLGFLGSSSVFCKDCRKVWKRSGAWFYKGIPKFILPLKIPGQADHPSFRPLPVEPAEQEPRSTETSRVYTWARGRVVSSDSDSDSDLSSSSLDDRLRPAGVRDPKGNKPWGESGGSVESLKMGPTRPASCLSGSQSSLASETGTGSADPRGAPAPWLDPEGPGEYQATFQPAHLLPVCPPPLWAAPLHVACLVLTCAPTLESARGCFQFPPFTERCARCWGNPGEPDPSLPTMNPDLKADHLCLFSAVISILSFIKCNLSKYFTVPVRVTGTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSL8</accession>
    <name>A0A4W2DSL8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSL8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036979.1">
        <property type="protein sequence ID" value="ENSBIXP00000022040.1"/>
        <property type="gene ID" value="ENSBIXG00000025002.1"/>
    </dbReference>
    <dbReference type="OMA" id="WRAVIYG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021389080" description="RNA-directed DNA polymerase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="434" mass="49942" checksum="73B612F18CD6F9F0" modified="2019-09-18" version="1">MLGIQTITNILLLSLISHATKVMLKILQARLQQYVNCELPDVKAGFRKGRGTRNQIANIHWIMEKAREFQKNIYFCFIDYAKAFEDVDHNKLWKILQEMAISDYLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYMMRNTGLEEAQAGIKIAGRNINNLRYADDTTLMAASEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSDFIFGGSKITTDGDCSHEIKRRLLLGRKVMTNLDSIFKSRDIILPTMVHLVKAMVFPVVMYGYDSWTVKKAEHPRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEALMLKLKLQYFGHLMRRVDSLKKTLMLGGIGGRRRRGQQRMRWLDGITDLMDVSLNELWELVMDREAWRAVIYGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXG5</accession>
    <name>A0A4W2DXG5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXG5"/>
    <dbReference type="STRING" id="30522.A0A4W2DXG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007872.1">
        <property type="protein sequence ID" value="ENSBIXP00000029204.1"/>
        <property type="gene ID" value="ENSBIXG00000010753.1"/>
    </dbReference>
    <dbReference type="OMA" id="HQDYIFY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11254">
        <property type="entry name" value="Sema_3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF27">
        <property type="entry name" value="SEMAPHORIN-3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021195648" evidence="10">
        <location>
            <begin position="19"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="12">
        <location>
            <begin position="31"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="619"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="707"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="758"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="707"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="761"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="793" mass="89194" checksum="367AF2C4B342F69E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZR6</accession>
    <name>A0A4W2DZR6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SDS3 homolog, SIN3A corepressor complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SUDS3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZR6"/>
    <dbReference type="STRING" id="30522.A0A4W2DZR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017252.1">
        <property type="protein sequence ID" value="ENSBIXP00000032330.1"/>
        <property type="gene ID" value="ENSBIXG00000014871.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033895.1">
        <property type="protein sequence ID" value="ENSBIXP00005040800.1"/>
        <property type="gene ID" value="ENSBIXG00005023607.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144281"/>
    <dbReference type="OMA" id="YERRWYH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="149"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="432" mass="49266" checksum="D18A6656780D0A7A" modified="2019-09-18" version="1">MAESGGRQAARDPEVAAGARPPLPRAGARGGAWRRRGRGRRRSGSWSRRLPQALRRGRRAWRAGGCGSRGARSASGGGDMSAAALLAPAPAPAGAPPAPEYYPEEDEELESAEDDERSCRGRESDEDTEDASETDLAKHDEEDFVEMKEQMYQDKLASLKRQLQQLQEGTLQEYQKRMKKLDQQYKERIRNAELFLQLETEQVERNYIKEKKAAVKEFEDKKVELKENLIAELEEKKKMIENEKLTMELTGDSMEVKPIMTRKLRRRPNDPVPIPDKRRKPAPAQLNYLLTDEQIMEDLRTLNKLKSPKRPASPSSPEHLPTTPAESPAQRFEARIEDGKLYYDKRWYHKSQAIYLESKDNQKLSCVISSVGANEIWVRKTSDSTKMRIYLGQLQRGLFVIRRRSVMIQRASQETTTKSYNAASLCLAKECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E006</accession>
    <name>A0A4W2E006_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Neurogenic locus notch homolog protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="24">Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOTCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2E006"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005063.1">
        <property type="protein sequence ID" value="ENSBIXP00000030098.1"/>
        <property type="gene ID" value="ENSBIXG00000006969.1"/>
    </dbReference>
    <dbReference type="OMA" id="ACPQVHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd21705">
        <property type="entry name" value="JMTM_Notch4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000185">
        <property type="entry name" value="basement membrane-specific heparan sulfate proteoglycan core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000142">
        <property type="entry name" value="Crumbs cell polarity complex component 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000136">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.320:FF:000001">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000152">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000456">
        <property type="entry name" value="Neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000647">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000738">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3310:FF:000004">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000651">
        <property type="entry name" value="neurogenic locus notch homolog protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000723">
        <property type="entry name" value="neurogenic locus notch homolog protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000060">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000092">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000125">
        <property type="entry name" value="Neurogenic locus notch protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000109">
        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000479">
        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000541">
        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000570">
        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000588">
        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000260">
        <property type="entry name" value="Notch receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000054">
        <property type="entry name" value="Slit guidance ligand 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008297">
        <property type="entry name" value="Notch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035993">
        <property type="entry name" value="Notch-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051355">
        <property type="entry name" value="Notch/Slit_guidance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022355">
        <property type="entry name" value="Notch_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010660">
        <property type="entry name" value="Notch_NOD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011656">
        <property type="entry name" value="Notch_NODP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836:SF23">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836">
        <property type="entry name" value="SLIT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06816">
        <property type="entry name" value="NOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07684">
        <property type="entry name" value="NODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00066">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002279">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01452">
        <property type="entry name" value="LNOTCHREPEAT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01983">
        <property type="entry name" value="NOTCH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01987">
        <property type="entry name" value="NOTCH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01338">
        <property type="entry name" value="NOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01339">
        <property type="entry name" value="NODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90193">
        <property type="entry name" value="Notch domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50258">
        <property type="entry name" value="LNR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0010">Activator</keyword>
    <keyword evidence="15 28" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="26" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="17 27 29" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 29" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 31" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021258965" description="Neurogenic locus notch homolog protein 4" evidence="31">
        <location>
            <begin position="22"/>
            <end position="1992"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
        <location>
            <begin position="22"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
        <location>
            <begin position="62"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
        <location>
            <begin position="116"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
        <location>
            <begin position="191"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
        <location>
            <begin position="231"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="32">
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        <recommendedName>
            <fullName evidence="10">Kelch domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">KLHDC3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(KLHDC3) complex specifically recognizes proteins with a glycine (Gly) at the C-terminus, leading to their ubiquitination and degradation: recognizes the C-terminal -Arg-(Xaa)n-Arg-Gly, -Arg-(Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly degrons. The CRL2(KLHDC3) complex mediates ubiquitination and degradation of truncated SELENOV and SEPHS2 selenoproteins produced by failed UGA/Sec decoding, which end with a glycine. May be involved in meiotic recombination process.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a CRL2(KLHDC3) complex, also named ECS(KLHDC3) complex, composed of CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter KLHDC3. May form oligomers as a KLHDC3-ELOB-ELOC complex; this interaction is likely autoinhibitory for the E3 ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027380372.1">
        <property type="nucleotide sequence ID" value="XM_027524571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3C4"/>
    <dbReference type="SMR" id="A0A4W2E3C4"/>
    <dbReference type="STRING" id="30522.A0A4W2E3C4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003560.1">
        <property type="protein sequence ID" value="ENSBIXP00000030598.1"/>
        <property type="gene ID" value="ENSBIXG00000012635.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012859.1">
        <property type="protein sequence ID" value="ENSBIXP00005028773.1"/>
        <property type="gene ID" value="ENSBIXG00005008566.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881554"/>
    <dbReference type="CTD" id="116138"/>
    <dbReference type="GeneTree" id="ENSGT00940000157952"/>
    <dbReference type="OMA" id="SQETYVF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140627">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000074">
        <property type="entry name" value="Kelch domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000136">
        <property type="entry name" value="Kelch domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052637">
        <property type="entry name" value="KLHDC3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="382" mass="43058" checksum="FCA0DADCB9294F08" modified="2019-09-18" version="1">MLRWTVHLEGGPRRVNHAAVAVGHRVYSFGGYCSGEDYETLRQIDVHIFNAVSLRWTKLPPVRPAARGQAPVVPYMRYGHSTVLIDDTVFLWGGRNDTEGACNVLYAFDVNTHKWSTPRVSGTVPGARDGHSACVLGKTMYIFGGYEQLADCFSNDIHKLDTSTMTWTLICTKGNPARWRDFHSATMLGSHMYVFGGRADRFGPFHSNNEIYCNRIRVFDTRTEAWLDCPPTPVLPEGRRSHSAFGYNGELYIFGGYNARLNRHFHDLWKFNPVSFTWKKIEPKGKGPCPRRRQCCCIVGDKIVLFGGTSPSPEEGLGDEFDLIDHSDLHILDFSPSLKTLCKLAVIQYNLDQSCLPHDIRWELNAMTTNSNISRPIVSSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3X4</accession>
    <name>A0A4W2E3X4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tigger transposable element derived 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TIGD5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tigger transposable element derived protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416263.1">
        <property type="nucleotide sequence ID" value="XM_027560462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3X4"/>
    <dbReference type="SMR" id="A0A4W2E3X4"/>
    <dbReference type="STRING" id="30522.A0A4W2E3X4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002982.1">
        <property type="protein sequence ID" value="ENSBIXP00000030813.1"/>
        <property type="gene ID" value="ENSBIXG00000012913.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015841.1">
        <property type="protein sequence ID" value="ENSBIXP00005027818.1"/>
        <property type="gene ID" value="ENSBIXG00005030024.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903877"/>
    <dbReference type="CTD" id="84948"/>
    <dbReference type="GeneTree" id="ENSGT00940000154420"/>
    <dbReference type="OMA" id="AYKCLAP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021556">
        <property type="term" value="P:central nervous system formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007379">
        <property type="term" value="P:segment specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000293">
        <property type="entry name" value="Tigger transposable element-derived protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF56">
        <property type="entry name" value="TIGGER TRANSPOSABLE ELEMENT-DERIVED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04218">
        <property type="entry name" value="CENP-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="7">
        <location>
            <begin position="52"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HTH CENPB-type" evidence="8">
        <location>
            <begin position="117"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="643" mass="70331" checksum="167DEB9E7A96DC9D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4F8</accession>
    <name>A0A4W2E4F8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4F8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002453.1">
        <property type="protein sequence ID" value="ENSBIXP00000030990.1"/>
        <property type="gene ID" value="ENSBIXG00000001285.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017208.1">
        <property type="protein sequence ID" value="ENSBIXP00005033462.1"/>
        <property type="gene ID" value="ENSBIXG00005014218.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016534"/>
    <dbReference type="OMA" id="GIQNAQR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040005">
        <property type="entry name" value="Polr1has"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41403">
        <property type="entry name" value="RCG43477-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41403:SF3">
        <property type="entry name" value="ZNRD1 ANTISENSE RNA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="209" mass="25029" checksum="34B48040FEFA9996" modified="2019-09-18" version="1">MVKEQEKGSEIKKRLEGKTTQSIERQKRYYLLPEREMKHIERHIHRGRRVREFKSKTFKQLPPHSSEIKFPRIVLGGHGIQNTQRRKQVNEREQMQIKDHRERMIRGRELVKQKLKEGILRKDQSQPPMHGKCESIRSIKKEIKEYESVIAYPLFQPCNSSRIKVNVLMEKSQNREENNTIIKPLQRRFLTMPPFLRSQIGKIKDLVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4M8</accession>
    <name>A0A4W2E4M8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase</fullName>
            <ecNumber evidence="9">3.6.1.62</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4M8"/>
    <dbReference type="SMR" id="A0A4W2E4M8"/>
    <dbReference type="STRING" id="30522.A0A4W2E4M8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002251.1">
        <property type="protein sequence ID" value="ENSBIXP00000031063.1"/>
        <property type="gene ID" value="ENSBIXG00000001311.1"/>
    </dbReference>
    <dbReference type="OMA" id="IHPVGET"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000097">
        <property type="entry name" value="Putative mRNA-decapping enzyme 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF5">
        <property type="entry name" value="MRNA-DECAPPING ENZYME 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="349"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="557" mass="60150" checksum="065BD4BDAB082E5D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E564</accession>
    <name>A0A4W2E564_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Poly(A) polymerase</fullName>
            <ecNumber evidence="14">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PAPOLA</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Polymerase that creates the 3'-poly(A) tail of mRNA's.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="14"/>
    <dbReference type="RefSeq" id="XP_027376940.1">
        <property type="nucleotide sequence ID" value="XM_027521139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E564"/>
    <dbReference type="SMR" id="A0A4W2E564"/>
    <dbReference type="STRING" id="30522.A0A4W2E564"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016061.1">
        <property type="protein sequence ID" value="ENSBIXP00000031887.1"/>
        <property type="gene ID" value="ENSBIXG00000001679.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046465.1">
        <property type="protein sequence ID" value="ENSBIXP00005035879.1"/>
        <property type="gene ID" value="ENSBIXG00005017204.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879549"/>
    <dbReference type="CTD" id="10914"/>
    <dbReference type="GeneTree" id="ENSGT00940000154598"/>
    <dbReference type="OMA" id="PAYPAMC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180011">
        <property type="term" value="P:cytosolic mRNA polyadenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="739" mass="82441" checksum="7C89C15E33232CFF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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        <alternativeName>
            <fullName evidence="13">Neurofilament triplet H protein</fullName>
        </alternativeName>
    </protein>
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        <name evidence="19" type="primary">NEFH</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro). Forms heterodimers with INA (in vitro).</text>
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        <subcellularLocation>
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            <location evidence="2">Axon</location>
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        <subcellularLocation>
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        </subcellularLocation>
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        <text evidence="15">Belongs to the intermediate filament family.</text>
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    <keyword evidence="6 15" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="IF rod" evidence="18">
        <location>
            <begin position="92"/>
            <end position="406"/>
        </location>
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        <location>
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        <location>
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            <end position="1081"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="308" mass="33949" checksum="12CD6367F6A424CD" modified="2019-09-18" version="1">MSHPSAPPPYEDRSPLYPGSPPPGGYGQPSVMPGGYPAYPQPGYGHPAGYPQPMPPVHPMPMNYGAGHGYDGGERAVSDTFGSGDWDDRKVRHAFIRKVYTIISIQLLVTVGIIAVFTFVKPVGDFVRANMAVYYASYAVFLVTYLTLACCQGPRRRFPWNIILLIIFTLAMAYMTGTISSVYKTKAVIIAMIITAVVSISVTIFCFQTKVDFTSCTGLFCVLGIVMTVTGIITVIVLVFKYVYWLHMVYAALGAICFTLFLAYDTQMVLGNRKHTISPEDYITGALQIYTDIVYIFTFVLQLVGSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBI2</accession>
    <name>A0A4W2EBI2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Elongation factor Ts, mitochondrial</fullName>
            <shortName evidence="6">EF-Ts</shortName>
            <shortName evidence="6">EF-TsMt</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">TSFM</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBI2"/>
    <dbReference type="SMR" id="A0A4W2EBI2"/>
    <dbReference type="STRING" id="30522.A0A4W2EBI2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026920.1">
        <property type="protein sequence ID" value="ENSBIXP00000036480.1"/>
        <property type="gene ID" value="ENSBIXG00000019961.1"/>
    </dbReference>
    <dbReference type="OMA" id="QEYMLDD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14275">
        <property type="entry name" value="UBA_EF-Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000031">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.20:FF:000007">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.20:FF:000008">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="entry name" value="Elongation factor Ts, dimerisation domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="ELONGATION FACTOR TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741:SF0">
        <property type="entry name" value="ELONGATION FACTOR TS, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25025">
        <property type="entry name" value="EF-Ts_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="Elongation factor Ts (EF-Ts), dimerisation domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFTs/EF1B dimerisation" evidence="7">
        <location>
            <begin position="175"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="383" mass="41817" checksum="E475A22401B2F113" modified="2019-09-18" version="1">MRPLDSRWRCPRSARSGAEFWPRCHAVGPWLHRCADRLHCTATGKMSLLRSLRLCLVARTGSCPLSALGPGPLLPSLQAGLPLLQSPQQWHTFHSGSWLSSASSKELLMKLRRKTGYSFINCKKALETCGGDLKQAESWLHKQAQKEGWSKAARLHGRKTKEGLIGLLQEGDTTVLVEVNCETDFVSRNLKFQQLVQQVALGTLLHCQNLKDQLSTYSKGFLNSSELSELPAGPEREGSLKDQLALAIGKLGENMILKRAAWVKVPAGFYVGSYVHGAMHSPSLHNLVLGKYGALVICETSELKANLADLGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPHGVSVVDFVRFECGEGEDAADAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDB9</accession>
    <name>A0A4W2EDB9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">PDZ domain containing ring finger 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PDZRN4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDB9"/>
    <dbReference type="STRING" id="30522.A0A4W2EDB9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029331.1">
        <property type="protein sequence ID" value="ENSBIXP00000037521.1"/>
        <property type="gene ID" value="ENSBIXG00000004005.1"/>
    </dbReference>
    <dbReference type="OMA" id="TMEQDYY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06715">
        <property type="entry name" value="PDZ1-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06716">
        <property type="entry name" value="PDZ2-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16719">
        <property type="entry name" value="RING-HC_LNX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000214">
        <property type="entry name" value="E3 ubiquitin-protein ligase PDZRN3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000028">
        <property type="entry name" value="PDZ domain containing ring finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000133">
        <property type="entry name" value="PDZ domain containing ring finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF6">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="225"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="403"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="519"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="723"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="660"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="723"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="737"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="766"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="806"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1037" mass="117394" checksum="2C975F999E21621E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFK5</accession>
    <name>A0A4W2EFK5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Potassium voltage-gated channel subfamily A member 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Voltage-gated potassium channel subunit Kv1.7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCNA7</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterotetramer of potassium channel proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homotetramer and heterotetramer of potassium channel proteins. Interacts with KCNAB1 and KCNAB2. Interacts with DLG1, DLG2 and DLG4 via their PDZ domains. Interacts with SIGMAR1. Detected in a complex with KCNA1. Interacts with KCNA2. Part of a complex containing KCNA1, KCNAB1 and LGI1. Interacts (via cytoplasmic N-terminal domain) with KCNRG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.7/KCNA7 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027370990.1">
        <property type="nucleotide sequence ID" value="XM_027515189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFK5"/>
    <dbReference type="SMR" id="A0A4W2EFK5"/>
    <dbReference type="STRING" id="30522.A0A4W2EFK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045891.1">
        <property type="protein sequence ID" value="ENSBIXP00000034946.1"/>
        <property type="gene ID" value="ENSBIXG00000018058.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039912.1">
        <property type="protein sequence ID" value="ENSBIXP00005043453.1"/>
        <property type="gene ID" value="ENSBIXG00005027144.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876218"/>
    <dbReference type="CTD" id="3743"/>
    <dbReference type="GeneTree" id="ENSGT00940000162339"/>
    <dbReference type="OMA" id="MPFDDPF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18408">
        <property type="entry name" value="BTB_POZ_KCNA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000028">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000122">
        <property type="entry name" value="potassium voltage-gated channel subfamily A member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048219">
        <property type="entry name" value="KCNA7_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF155">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="22">
        <location>
            <begin position="13"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="456" mass="50473" checksum="AC2D8862EB4A6698" modified="2019-09-18" version="1">MEPGCPPPCGCCERVVLNVAGLRFETRARTLGRFPDTLLGDPVRRSRFYDGARREYFFDRHRPSFDAVLYYYQSGGRLRRPAHVPLDVFLEEVAFYGLGGAALARLREDEGCPLPTERPLPRHAFARQLWLLFEFPESSQAARVLAVVSVLVILVSIVVFCLETLPDFRDDRDNPGLAAVAAAGPFPARLNGSSPVPGPLPRLPFDDPFFVVETLCICWFSFELLVRLAACPSKTAFFKNVMNLIDFVAILPYFVALGTELARQRGVGQPAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLRASMRELGLLIFFLFLGVVLFSSAVYFAEVDRADSHFTSIPESFWWAVVTMTTVGYGDMAPVTVGGKIVGSLCAIAGVLTISLPVPVIVSNFSYFYHRETDDEEAGMYSHVDTQPCGPLEGKVNGGLVDPEVSELPPPLWAPPGKHMVTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHK8</accession>
    <name>A0A4W2EHK8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dedicator of cytokinesis 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DOCK10</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EHK8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026100.1">
        <property type="protein sequence ID" value="ENSBIXP00000036132.1"/>
        <property type="gene ID" value="ENSBIXG00000019547.1"/>
    </dbReference>
    <dbReference type="OMA" id="RACNTPP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060997">
        <property type="term" value="P:dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08697">
        <property type="entry name" value="C2_Dock-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11699">
        <property type="entry name" value="DHR2_DOCK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13267">
        <property type="entry name" value="PH_DOCK-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000016">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000015">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037809">
        <property type="entry name" value="C2_Dock-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF71">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="194"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="6">
        <location>
            <begin position="685"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="7">
        <location>
            <begin position="1703"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1304"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1315"/>
            <end position="1326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2199" mass="251603" checksum="039E3BD3D087FAFD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EI05</accession>
    <name>A0A4W2EI05_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPATA31-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SPATA31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EI05"/>
    <dbReference type="STRING" id="30522.A0A4W2EI05"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046938.1">
        <property type="protein sequence ID" value="ENSBIXP00000036375.1"/>
        <property type="gene ID" value="ENSBIXG00000006375.1"/>
    </dbReference>
    <dbReference type="OMA" id="HWINAEM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027970">
        <property type="entry name" value="SPATA31-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21859">
        <property type="entry name" value="ACROSOME-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21859:SF15">
        <property type="entry name" value="PROTEIN SPATA31F1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15371">
        <property type="entry name" value="DUF4599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="SPATA31-like" evidence="8">
        <location>
            <begin position="52"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="194" mass="22284" checksum="68FA365AC4E4401C" modified="2019-09-18" version="1">MLSPTFILWDVGYPFYTYGSIIIIALIIWQVKKNHRGLKLGPNRNCCRRHQKVKQRAKEKTPRARRHSRKEADKPLELLSIMRSQGWLPQEGSVRRLLCADPCCQICNSVALEIQQLISAVEADLGNKLKHFTQWINPDMKGQGRKECILRCKDEKVAKTKTKKAEKSPPSTKRPMKGAKLEKEEGFFDAPSAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJ97</accession>
    <name>A0A4W2EJ97_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="5">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SCAMP2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCAMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377861.1">
        <property type="nucleotide sequence ID" value="XM_027522060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJ97"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019214.1">
        <property type="protein sequence ID" value="ENSBIXP00000033169.1"/>
        <property type="gene ID" value="ENSBIXG00000002422.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008966.1">
        <property type="protein sequence ID" value="ENSBIXP00005030075.1"/>
        <property type="gene ID" value="ENSBIXG00005000787.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880048"/>
    <dbReference type="CTD" id="10066"/>
    <dbReference type="GeneTree" id="ENSGT00940000156476"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF7">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="328" mass="36703" checksum="0E5578B7ACA3932C" modified="2019-09-18" version="1">MSSFDTNPFADPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVPVSQMPGASQPAVLQPSVEPTQPTPQAVASAAQASLLRQQEELDRKAAELERKERELQNTVANLHVRENNWPPLPVWCPVKPCFYQDFSTEIPADYQRICKMLYYLWMLHSVTLFLNLLACLAWFLVDTSRGVDFGLSILWFVIFTPCAFLCWYRPIYKAFRSDNSFSFFVFFFVFFCQIGIYIIQLIGIPSLGDSGWIAALSTMKHNLAVSVIMMVVAGFFTLCAVLSLFLLKRVHSLYRRTGASFQQAQEEFSQGIFSSRTFRSAASSAAQGAFQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKB6</accession>
    <name>A0A4W2EKB6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
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            <begin position="822"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="904"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1105" mass="113858" checksum="728052281A46FD76" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EN91</accession>
    <name>A0A4W2EN91_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease A-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EN91"/>
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        <property type="protein sequence ID" value="ENSBIXP00000038307.1"/>
        <property type="gene ID" value="ENSBIXG00000022104.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EDDM3A/EDDM3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023412">
        <property type="entry name" value="RNaseA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16788">
        <property type="entry name" value="EPIDIDYMAL SECRETORY PROTEIN E3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16788:SF0">
        <property type="entry name" value="EPIDIDYMAL SECRETORY PROTEIN E3-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00074">
        <property type="entry name" value="RnaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNAse_Pc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54076">
        <property type="entry name" value="RNase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021341077" description="Ribonuclease A-domain domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease A-domain" evidence="4">
        <location>
            <begin position="26"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="146" mass="17583" checksum="1730D87C10025273" modified="2019-09-18" version="1">MASTLKALGSLLALLFPLCGLLVHSQNLSWREFMKQHHLSTNWEFSKYKCNDLMRERGIPKNKNYHIFIYTLWHKIVHICMRNWGDRHRNVYIWATYPFEVLKCIRKNSRGNYKDYKSYSYIEFHCSRNAFVDGIEDMRLLEDISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EN99</accession>
    <name>A0A4W2EN99_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113899425</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EN99"/>
    <dbReference type="STRING" id="30522.A0A4W2EN99"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030956.1">
        <property type="protein sequence ID" value="ENSBIXP00000038212.1"/>
        <property type="gene ID" value="ENSBIXG00000021994.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR11G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF364">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="46"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="35035" checksum="4A211DE065EF71DC" modified="2019-09-18" version="1">SSPNSMNVSGVNTVTEFILLSFPCSREGQILLFMLFSALYLLTLMGNGSIICAVCWDQRLHTPMYILLANFSFLEIWYVTSTVPNMLANFLSDNKFISFSGCFLQFYFFFSLGSTECFFLAIMAFDRYLAICWPLHYPTLMTGRLCANLVISCWVLGFLWFLIPIIIISQMSFCGSRIIDHFLCDPGPLLALTCTRVPVIELTSSTLSSLLLFIPFLFTMVSYALVLQAVLKFPSSAGRRKAFSTCGSHLTVVSLFYGSVMVMYVSPTSEHDAGMQKIVTLFYSVVTPLINPVIYSLRNKDMKHAMKKLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ER42</accession>
    <name>A0A4W2ER42_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc transporter ZIP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Solute carrier family 39 member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Zrt- and Irt-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SLC39A3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29352"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ER42"/>
    <dbReference type="SMR" id="A0A4W2ER42"/>
    <dbReference type="STRING" id="30522.A0A4W2ER42"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033780.1">
        <property type="protein sequence ID" value="ENSBIXP00000039453.1"/>
        <property type="gene ID" value="ENSBIXG00000006918.1"/>
    </dbReference>
    <dbReference type="OMA" id="HHHGHFN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF221">
        <property type="entry name" value="ZINC TRANSPORTER ZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="34005" checksum="B7D2EB3D567AC53B" modified="2019-09-18" version="1">MVKLLVAKILCMVGMFFFMLLGSLLPVKIIEMDFEKAHRSKKILSLCNTFGGGVFLATCFNALLPAVREKLKEVLTLAHISTDYPLAETIMLLGFFMTVFLEQLVLTFRKERPAFIDLETFNASSDAGSDSEYESPFMGGPRGHALYAEPHGHSHGLSVQELSRSSPLRLLSLVFALSAHSVFEGLALGLQEEGEKVVSLFVGVAIHETLVAVALGINMARSAMALRDAAKLAVTVSAMIPLGISLGLGIESAQGMPSSVASVLLQGLAGGTFLFVTFFEILAKELEEKSDRLLKVLFLVLGYTVLAGMVFIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ER45</accession>
    <name>A0A4W2ER45_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ER45"/>
    <dbReference type="STRING" id="30522.A0A4W2ER45"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033255.1">
        <property type="protein sequence ID" value="ENSBIXP00000039212.1"/>
        <property type="gene ID" value="ENSBIXG00000023174.1"/>
    </dbReference>
    <dbReference type="OMA" id="GWKNAQT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="140"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="405"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="441" mass="49040" checksum="AAEE76C8BB2C7679" modified="2019-09-18" version="1">MALALMCGRRQLLRLLRPQRQFHSVAGPCQWPRKPLTAGLGFPADRCLGHPRYVLLMAPGPRGLSTSAVSFGEAQVPAPPVIPATPPPTAVPEVASGEAADVIQAAAEQSFAELGLGSYTPVGLIQNLLEFMHVNLGLPWWGAIAACTVLARCLVFPLIVKGQREAAKIHNHLPEIQKFSARIREAKLTGNHTEFYRASSEMTFYQKKHDIKLFRPLILPLTQAPIFISFFIALREMANLPVPSLQTGGLWWFQDLTLSDPIYVLPLVVTATMWGVLELGAETGMQSSDLQWMRNFIRLMPLAVLPITIHFPTAVFMYWLSSNMFSLGQVACLRIPAVRTVLKIPQRVVHDPDKLAPREGFLKSFKQGWKNAEMAHQLQERERRMQNHLELAARGPLRQTFAHNPLLQHGKNDPPNTPNSSSSSSSSNKAKSKQPWRDTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ES34</accession>
    <name>A0A4W2ES34_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ES34"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034650.1">
        <property type="protein sequence ID" value="ENSBIXP00000039820.1"/>
        <property type="gene ID" value="ENSBIXG00000023806.1"/>
    </dbReference>
    <dbReference type="OMA" id="HICIETR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="42626" checksum="E4D102BF45A3F17B" modified="2019-09-18" version="1">MREIEENKNKWKHIPCSSMKRLKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVHRGCILSPCLFNLYAEYIMRNPGLEETQAGLKTAGRNINHLRYADDTTLMAESEEELQSLLMKVKVESEKVGLKLNIQKTKIMASSPITSWEIDGETVSDFIFLGSTITADGDCSHEIKRRLLLGRKVMTNLDSILKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAEHRRIDAFELWCWRRLWRVPWTTRRSNQSILKEISPGISLEGVMLKLKLQYVGHLMRNIDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVMDREAWCAAIHGVAKSRTGLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESM3</accession>
    <name>A0A4W2ESM3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Preautophagosomal structure membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESM3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035125.1">
        <property type="protein sequence ID" value="ENSBIXP00000040030.1"/>
        <property type="gene ID" value="ENSBIXG00000024073.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF14">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="739"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="848"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="880"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="890"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="924" mass="100891" checksum="079AB77EDA92BD84" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVM9</accession>
    <name>A0A4W2EVM9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Shroom family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SHROOM3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EVM9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037010.1">
        <property type="protein sequence ID" value="ENSBIXP00000040837.1"/>
        <property type="gene ID" value="ENSBIXG00000024996.1"/>
    </dbReference>
    <dbReference type="OMA" id="QRCAVPD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06750">
        <property type="entry name" value="PDZ_shroom2_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000100">
        <property type="entry name" value="Shroom family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014800">
        <property type="entry name" value="ASD1_dom"/>
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    <dbReference type="InterPro" id="IPR014799">
        <property type="entry name" value="ASD2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF33">
        <property type="entry name" value="PROTEIN SHROOM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08688">
        <property type="entry name" value="ASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08687">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51306">
        <property type="entry name" value="ASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="25"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="ASD1" evidence="12">
        <location>
            <begin position="934"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="ASD2" evidence="13">
        <location>
            <begin position="1661"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="350"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="619"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="697"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="788"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1143"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="748"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="825"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="845"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="891"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="902"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1183"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1372"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1424"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1445"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1510"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1582"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1600"/>
            <end position="1620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1988" mass="215166" checksum="654A2D31FBBF33CE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWC7</accession>
    <name>A0A4W2EWC7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="10">Replication protein A 14 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Replication factor A protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RPA3</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395878.1">
        <property type="nucleotide sequence ID" value="XM_027540077.1"/>
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    <dbReference type="SMR" id="A0A4W2EWC7"/>
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        <property type="protein sequence ID" value="ENSBIXP00000041347.1"/>
        <property type="gene ID" value="ENSBIXG00000025592.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046188.1">
        <property type="protein sequence ID" value="ENSBIXP00005035548.1"/>
        <property type="gene ID" value="ENSBIXG00005016720.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891691"/>
    <dbReference type="CTD" id="6119"/>
    <dbReference type="GeneTree" id="ENSGT00390000008029"/>
    <dbReference type="OMA" id="HEFPEYY"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:damaged DNA binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:DNA replication"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:nucleotide-excision repair"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication protein A 14 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114:SF1">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114">
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    </dbReference>
    <dbReference type="Pfam" id="PF08661">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="121" mass="13508" checksum="A26F8F8165E9A3B6" modified="2019-09-18" version="1">MVDVMESPKARINASMLAQFIDQPVCFVGRLEKIHPTGKMFILSDGEGKNVTIELMEPLDEEISGIVEVVGRVTAKATIMCASYVQFKEDNHPFDLGLYNEAVKITHEFPQFFPLGVVQYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWD4</accession>
    <name>A0A4W2EWD4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSBIXT00000037546.1">
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        <property type="gene ID" value="ENSBIXG00000004991.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="336" mass="38482" checksum="5041E7FBAEA55503" modified="2019-09-18" version="1">MGIPDHLICLLRNLYVGQEATVRTGHGTTDWFQIGKGVRQGCMLSPCLFNFYAEYIMRNAGLEETQAGIKIARRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPTTSRELDEETVETVSDFIFLDSKITTDGDFSHEIKRCLLLGRKVITNLDSIFKSRDITLPTKVRLVKAMVFPLVMYGCESWTVKKAERQRIDAFELWCWRRLLRVPWTARRSNQSNLKEISPGISLEGMILKLKLQSFGHLMRRVDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLGELRELVLDREAWRAVLHGVVKSQTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWP2</accession>
    <name>A0A4W2EWP2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Krueppel-like factor 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EWP2"/>
    <dbReference type="SMR" id="A0A4W2EWP2"/>
    <dbReference type="STRING" id="30522.A0A4W2EWP2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038328.1">
        <property type="protein sequence ID" value="ENSBIXP00000041461.1"/>
        <property type="gene ID" value="ENSBIXG00000002045.1"/>
    </dbReference>
    <dbReference type="OMA" id="FQGCIKV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21573">
        <property type="entry name" value="KLF16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000595">
        <property type="entry name" value="Krueppel-like factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000232">
        <property type="entry name" value="Krueppel-like factor 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF71">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="202"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="252" mass="25636" checksum="EBEE5B02779DB26D" modified="2019-09-18" version="1">MSAAVACVDYFAADVLMAISSGAVVHRGRPGPEGAGPATGLDVRAPRREAAPPGPPGPPPPAPAVLGPGPGAAAAAPHLLAASILADLRGGPSAAPGGASPASSSSAASSPSSGRAPGAAPAAAKSHRCPFPGCAKAYYKSSHLKSHLRTHTGERPFACDWPGCDKKFARSDELARHHRTHTGEKRFPCPLCSKRFTRSDHLTKHARRHPGFRPELLRRQGTRSTSPSDSLPCSLAGSPAPSPVPSPAPVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EX81</accession>
    <name>A0A4W2EX81_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Taste receptor type 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor T2R family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EX81"/>
    <dbReference type="STRING" id="30522.A0A4W2EX81"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050897.1">
        <property type="protein sequence ID" value="ENSBIXP00000041695.1"/>
        <property type="gene ID" value="ENSBIXG00000005289.1"/>
    </dbReference>
    <dbReference type="OMA" id="ATWFATC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033038">
        <property type="term" value="F:bitter taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15024">
        <property type="entry name" value="7tm_TAS2R42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000042">
        <property type="entry name" value="Taste receptor type 2 member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007960">
        <property type="entry name" value="TAS2R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394:SF78">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05296">
        <property type="entry name" value="TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3 12" id="KW-0919">Taste</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="318" mass="36205" checksum="9C287283406070FC" modified="2019-09-18" version="1">MPSGIENTFLAATIGGFLIGMLGNGFIVLVNCIDLVKRQKLSSADCILTGLAISRISQLWVILCDSFLLVLWPHLYAIDKLTKVVSSFWILSNHLATWFATCLSVFYFFKVASFSHPCFTWLRWQIRSVVLVLLLGSLSLLFLNYESIYTLSHILTNSYKIYVRNSTWSSDVSETHYLHQLIVFNFINLIPFLLSLTSLLLLVLSLMRHIRNLQLNPSSKDLSTEAHKRAMKIVMSFLFLFIIHVSSILLIAWVFLKLQGRLAQLVVVLTSTVFPSSHSFILILGNSKLRQNALRLLWYLNCHPKRNVSASHKEQSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1Q6</accession>
    <name>A0A4W2F1Q6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="19">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + NAD(+) = 20-oxo-(5Z,8Z,11Z,14Z)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39799"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:76624"/>
            <dbReference type="ChEBI" id="CHEBI:76645"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:39800"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>20-oxo-(5Z,8Z,11Z,14Z)-eicosatetraenoate + NAD(+) + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenedioate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:39803"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:76645"/>
            <dbReference type="ChEBI" id="CHEBI:76647"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39804"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>S-nitrosoglutathione + NADH + H(+) = S-(hydroxysulfenamide)glutathione + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:78371"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:78372"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F1Q6"/>
    <dbReference type="SMR" id="A0A4W2F1Q6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051844.1">
        <property type="protein sequence ID" value="ENSBIXP00000042963.1"/>
        <property type="gene ID" value="ENSBIXG00000006840.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080007">
        <property type="term" value="F:S-nitrosoglutathione reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF4">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0520">NAD</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="20">
        <location>
            <begin position="17"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="374" mass="39677" checksum="307A47ECEC560C0F" modified="2019-09-18" version="1">MANQVIKCKAAVAWEAGKPLSIEEVEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGNYPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAALNAAKVEPGSTCAVFGLGGVGLAVIMGCKMAGAARIIGVDINKDKFARAKEFGASECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGISVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHSLPFDQINEAFDLMHAGKSIRTVVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F364</accession>
    <name>A0A4W2F364_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fatty acid-binding protein, intestinal</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Fatty acid-binding protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Intestinal-type fatty acid-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F364"/>
    <dbReference type="SMR" id="A0A4W2F364"/>
    <dbReference type="STRING" id="30522.A0A4W2F364"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042967.1">
        <property type="protein sequence ID" value="ENSBIXP00000043913.1"/>
        <property type="gene ID" value="ENSBIXG00000028098.1"/>
    </dbReference>
    <dbReference type="OMA" id="FMEAMGV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19445">
        <property type="entry name" value="FABP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031272">
        <property type="entry name" value="FABP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="12">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="132" mass="15036" checksum="282112D7A885BF68" modified="2019-09-18" version="1">MAFDGTWKVDRNENYEKFMEKMGINVVKRKLAAHDNLKLIITQEGNKFTVKESSTFRSIEIIFELGVTFNYSLADGTELSGAWALEGDKLVGKFKRLDNGNALNTVREIIGGEMVQTYTYEGVEAKRIFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F489</accession>
    <name>A0A4W2F489_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Mucolipin TRP cation channel 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MCOLN2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="12">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="417"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="485"/>
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        <location>
            <begin position="90"/>
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        <location>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <sequence length="566" mass="65596" checksum="4FDA4B5F4FA717EC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F5C7</accession>
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        <submittedName>
            <fullName evidence="16">Secreted frizzled related protein 4</fullName>
        </submittedName>
    </protein>
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        <name evidence="16" type="primary">SFRP4</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="10">Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.</text>
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            <location evidence="1">Secreted</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="component" value="Chromosome 4"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of epidermal cell differentiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0002092">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of BMP signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
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        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF7">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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    <feature type="chain" id="PRO_5044611288" evidence="13">
        <location>
            <begin position="19"/>
            <end position="346"/>
        </location>
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        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
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        <location>
            <begin position="178"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="291"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00055544"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000259" key="14">
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            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="346" mass="39573" checksum="C01209CE3A3FF9E5" modified="2019-09-18" version="1">MLLSILTALCLWLRLALGVRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPDDVKWIDITPDMMVQERPLDIDCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQQRTAQDKKRTAGRTSRSNAPKPKGKPPAPKPASPKKNIKARSAPKSTNPKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FQC7</accession>
    <name>A0A4W2FQC7_BOBOX</name>
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        <submittedName>
            <fullName evidence="6">Leucine rich repeat containing 58</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LRRC58</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_027530332.1"/>
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    <dbReference type="SMR" id="A0A4W2FQC7"/>
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    <dbReference type="GeneTree" id="ENSGT00940000156556"/>
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        <property type="component" value="Chromosome 1"/>
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    </dbReference>
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        <property type="entry name" value="LRR-SigEffector_domain"/>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752:SF13">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="7"/>
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        <property type="entry name" value="L domain-like"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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            <begin position="122"/>
            <end position="196"/>
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            <begin position="342"/>
            <end position="361"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="371" mass="40596" checksum="740B0504DBEC3A09" modified="2019-09-18" version="1">MEEVGGAVATAGEAELNLSRLSVSLETLESELEARGEERRGAREALLRLLLPHNRLVSLPRALGSGFPHLQLLDVSGNALTALGPELLALRGLRTLLAKNNRLGGPGAFPKGLAQSPLCRSLQVLNLSGNCFQEVPTSLLELRALQTLSLGGNQLQSIPAEIENLRSLECLYLGGNFIKEIPPELGNLPSLNYLVLCDNKIQSVPPQLSQLHSLRSLSLHNNLLTYLPREILNLIHLEELSLRGNPLVVRFVRDLTYDPPTLLELAARTIKIRNISYTPYDLPGNLLRYLSLASNCPNPKCGGVYFDCCVRQIKFVDFCGKYRLPLMHYLCSPECSSPCSSASHSSTSQSESDSEDEASVAAHRMQKVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FX59</accession>
    <name>A0A4W2FX59_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spindlin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPIN1</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIN/STSY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027406185.1">
        <property type="nucleotide sequence ID" value="XM_027550384.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027406186.1">
        <property type="nucleotide sequence ID" value="XM_027550385.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2FX59"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000034349.1">
        <property type="protein sequence ID" value="ENSBIXP00000039713.1"/>
        <property type="gene ID" value="ENSBIXG00000004701.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044952.1">
        <property type="protein sequence ID" value="ENSBIXP00005009959.1"/>
        <property type="gene ID" value="ENSBIXG00005014731.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897583"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182925"/>
    <dbReference type="OMA" id="CMCEYRK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009303">
        <property type="term" value="P:rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Spindlin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spindlin/Ssty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003671">
        <property type="entry name" value="SPIN/Ssty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042567">
        <property type="entry name" value="SPIN/Ssty_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10405">
        <property type="entry name" value="SPINDLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02513">
        <property type="entry name" value="Spin-Ssty"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="262" mass="29588" checksum="05C13DE0E26EFD8D" modified="2019-09-18" version="1">MKTPFGKTPGQRSRADAGHAGVSASMMKKRTSHKKHRSSVGPSKPVSQPRRNIVGCRIQHGWKEGNGPVTQWKGTVLDQVPVNPSLYLIKYDGFDCVYGLELNKDERVSALEVLPDRVATSRISDAHLADTMIGKAVEHMFETEDGSKDEWRGMVLARAPIMNTWFYITYEKDPVLYMYQLLDDYKEGDLRIMPDSNDSPPAEREPGEVVDSLVGKQVEYAKEDGSKRTGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FYW8</accession>
    <name>A0A4W2FYW8_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 202-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC113876140</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSBIXP00000035397.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005011228.1"/>
        <property type="gene ID" value="ENSBIXG00005015702.1"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="component" value="Chromosome 18"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="266" mass="29800" checksum="B07AE4236861A186" modified="2019-09-18" version="1">MKPLLQSSYCVQSPMSTVEPISSWEEDRKFVLRAWCLVFIILATAMLLSVLDGRMAYLHGAYTGYVGFWTNCKKHTCADLRQVTVLIHMSMGFMILAVILALVLLLAMGFSFRPALRRLNKTDLVFSTLSSFTVMQGGRGCLWLASLSGGLWVPVQPRVHLWPHLSAGLLILLSLTLFIANCEMLKPRPQVSYLVTTYLSWGASALMLWAGILSYLNYMGMWGKGTSSTERRMSYRRWASLQNTRKSISEQLSSDAGSKHAEDLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FZD9</accession>
    <name>A0A4W2FZD9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromodomain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BRD2</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with E2F1. Interacts with (acetylated) STAT3; promoting STAT3 recruitment to chromatin. Interacts with CTCF; promoting BRD2 recruitment to chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the BET family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027525134.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027525135.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027525136.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027525137.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027525138.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380940.1">
        <property type="nucleotide sequence ID" value="XM_027525139.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2FZD9"/>
    <dbReference type="STRING" id="30522.A0A4W2FZD9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006146.1">
        <property type="protein sequence ID" value="ENSBIXP00000005249.1"/>
        <property type="gene ID" value="ENSBIXG00000011489.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018058.1">
        <property type="protein sequence ID" value="ENSBIXP00005009894.1"/>
        <property type="gene ID" value="ENSBIXG00005014704.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881989"/>
    <dbReference type="CTD" id="6046"/>
    <dbReference type="GeneTree" id="ENSGT00940000153385"/>
    <dbReference type="OMA" id="GGMDQHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140033">
        <property type="term" value="F:acetylation-dependent protein binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:histone H3K14ac reader activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:histone H4K12ac reader activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000330">
        <property type="term" value="P:positive regulation of T-helper 17 cell lineage commitment"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071168">
        <property type="term" value="P:protein localization to chromatin"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
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        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
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        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF240">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="91"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="364"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="18">
        <location>
            <begin position="634"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="268"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="456"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="739"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="481"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022853"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040998"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044509"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="803" mass="88122" checksum="DE0F35E3A3A55D2A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GAC6</accession>
    <name>A0A4W2GAC6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Interferon regulatory factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">IRF1</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Monomer. Homodimer. Interacts with EP300. Interacts with MYD88. Interacts with PIAS3. Interacts with SPOP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the IRF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402882.1">
        <property type="nucleotide sequence ID" value="XM_027547081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GAC6"/>
    <dbReference type="SMR" id="A0A4W2GAC6"/>
    <dbReference type="STRING" id="30522.A0A4W2GAC6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034140.1">
        <property type="protein sequence ID" value="ENSBIXP00000020187.1"/>
        <property type="gene ID" value="ENSBIXG00000023537.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023895.1">
        <property type="protein sequence ID" value="ENSBIXP00005013818.1"/>
        <property type="gene ID" value="ENSBIXG00005007805.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895645"/>
    <dbReference type="CTD" id="3659"/>
    <dbReference type="GeneTree" id="ENSGT00940000156288"/>
    <dbReference type="OMA" id="HSGYTIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043374">
        <property type="term" value="P:CD8-positive, alpha-beta T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035458">
        <property type="term" value="P:cellular response to interferon-beta"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045590">
        <property type="term" value="P:negative regulation of regulatory T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032728">
        <property type="term" value="P:positive regulation of interferon-beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032735">
        <property type="term" value="P:positive regulation of interleukin-12 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000564">
        <property type="term" value="P:regulation of CD8-positive, alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034124">
        <property type="term" value="P:regulation of MyD88-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060333">
        <property type="term" value="P:type II interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000065">
        <property type="entry name" value="Interferon regulatory factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017431">
        <property type="entry name" value="IRF1/IRF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF3">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038196">
        <property type="entry name" value="IFN_RF1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00601">
        <property type="entry name" value="IRF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="18">
        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="92"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="15">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="15">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="16">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038196-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038196-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="322" mass="36241" checksum="273CA27DBF5CA7BB" modified="2019-09-18" version="1">MPITRMRMRPWLEMQINSNQIPGLIWINKEEMIFQIPWKHAAKHGWDINKDACLFRSWAIHTGRYKAGEKEPDPKTWKANFRCAMNSLPDIEEVKDQSRNKGSSAVRVYRMLPPLTKSQRKERKSKSSRDARSKAKKKPYGEYSPDTFSDGLSSSTLPDDHSNYTVRSYMGQDLDIERTLTPALSPCGVSSTLPNWSIPVEIVPDSTSDLYNFQVSPMPSTSEAATDEDEEGKLTEDIMKLLEQTGWQQTSVDGKGYLLNEPGAQPTSVYGEFSCKEEPEVDSPGGYIGLISSDMKNMDPSWLDSLLTPVRLPSIQAIPCAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GLQ6</accession>
    <name>A0A4W2GLQ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylcholine:ceramide cholinephosphotransferase 1</fullName>
            <ecNumber evidence="13">2.7.8.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sphingomyelin synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SGMS1</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-(9Z-octadecenoyl)-2-acyl-sn-3-glycerol + a sphingomyelin = a 1-(9Z-octadecenoyl)-2-acyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:43320"/>
            <dbReference type="ChEBI" id="CHEBI:17636"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:78421"/>
            <dbReference type="ChEBI" id="CHEBI:82983"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:43321"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:43322"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N-hexadecanoyl-(4R)-hydroxysphinganine + a 1,2-diacyl-sn-glycero-3-phosphocholine = N-hexadecanoyl-(4R)-hydroxysphinganine-phosphocholine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:42140"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:65107"/>
            <dbReference type="ChEBI" id="CHEBI:78650"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:42141"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:42142"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N-hexadecanoylsphinganine + a 1,2-diacyl-sn-glycero-3-phosphocholine = N-hexadecanoyl-sphinganine-1-phosphocholine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:41796"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:67042"/>
            <dbReference type="ChEBI" id="CHEBI:78647"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41797"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:41798"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:18765"/>
            <dbReference type="ChEBI" id="CHEBI:17636"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="EC" id="2.7.8.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18766"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:18767"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acylsphing-4-enine 1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:36079"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:73203"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:36080"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.27" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027384740.1">
        <property type="nucleotide sequence ID" value="XM_027528939.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384741.1">
        <property type="nucleotide sequence ID" value="XM_027528940.1"/>
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    <dbReference type="RefSeq" id="XP_027384742.1">
        <property type="nucleotide sequence ID" value="XM_027528941.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384743.1">
        <property type="nucleotide sequence ID" value="XM_027528942.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384744.1">
        <property type="nucleotide sequence ID" value="XM_027528943.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384745.1">
        <property type="nucleotide sequence ID" value="XM_027528944.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384747.1">
        <property type="nucleotide sequence ID" value="XM_027528946.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384748.1">
        <property type="nucleotide sequence ID" value="XM_027528947.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384749.1">
        <property type="nucleotide sequence ID" value="XM_027528948.1"/>
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    <dbReference type="RefSeq" id="XP_027384750.1">
        <property type="nucleotide sequence ID" value="XM_027528949.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384751.1">
        <property type="nucleotide sequence ID" value="XM_027528950.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384752.1">
        <property type="nucleotide sequence ID" value="XM_027528951.1"/>
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    <dbReference type="RefSeq" id="XP_027384753.1">
        <property type="nucleotide sequence ID" value="XM_027528952.1"/>
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    <dbReference type="RefSeq" id="XP_027384754.1">
        <property type="nucleotide sequence ID" value="XM_027528953.1"/>
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    <dbReference type="RefSeq" id="XP_027384755.1">
        <property type="nucleotide sequence ID" value="XM_027528954.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384756.1">
        <property type="nucleotide sequence ID" value="XM_027528955.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384757.1">
        <property type="nucleotide sequence ID" value="XM_027528956.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384758.1">
        <property type="nucleotide sequence ID" value="XM_027528957.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384759.1">
        <property type="nucleotide sequence ID" value="XM_027528958.1"/>
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    <dbReference type="RefSeq" id="XP_027384760.1">
        <property type="nucleotide sequence ID" value="XM_027528959.1"/>
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        <property type="nucleotide sequence ID" value="XM_027528960.1"/>
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        <property type="nucleotide sequence ID" value="XM_027528962.1"/>
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    <dbReference type="STRING" id="30522.A0A4W2GLQ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001330.1">
        <property type="protein sequence ID" value="ENSBIXP00000031367.1"/>
        <property type="gene ID" value="ENSBIXG00000013614.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032937.1">
        <property type="protein sequence ID" value="ENSBIXP00005019848.1"/>
        <property type="gene ID" value="ENSBIXG00005023052.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884371"/>
    <dbReference type="CTD" id="259230"/>
    <dbReference type="GeneTree" id="ENSGT00940000158306"/>
    <dbReference type="OMA" id="HWPLRCP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000139">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0002950">
        <property type="term" value="F:ceramide phosphoethanolamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:ceramide biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:sphingomyelin biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09514">
        <property type="entry name" value="SAM_SGMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000040">
        <property type="entry name" value="Phosphatidylcholine:ceramide cholinephosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF28">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:CERAMIDE CHOLINEPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="22">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00038996"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042067"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049904"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050817"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051574"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="413" mass="48487" checksum="C33C88949693C171" modified="2019-09-18" version="1">MKEVVYWSPEKVADWLQENAMPEYCEPLEHFTGRDLINLTQEDFTKPPLCLVTSDNGQRLLHMIETLKMEHHLEAHKNGHANGHLSIGTGSPSPNGSFSIKVKPNGMPNGYRKEMIKIPMPEPERSQYPMEWGKTFLAFLYALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLIQWLLLKYKSIISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQLRRIMKLIAGGGLSITGSHNMCGDYLYSGHTVMLTLTYLFIKEYSPRRLWWYHWLCWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLFWWYHTMANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVIHLSRQVKYSRLVNDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H284</accession>
    <name>A0A4W2H284_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM118B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FAM118B</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in Cajal bodies formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAM118 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027387589.1">
        <property type="nucleotide sequence ID" value="XM_027531788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H284"/>
    <dbReference type="SMR" id="A0A4W2H284"/>
    <dbReference type="STRING" id="30522.A0A4W2H284"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014271.1">
        <property type="protein sequence ID" value="ENSBIXP00000027166.1"/>
        <property type="gene ID" value="ENSBIXG00000028679.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052633.1">
        <property type="protein sequence ID" value="ENSBIXP00005024783.1"/>
        <property type="gene ID" value="ENSBIXG00005027365.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885937"/>
    <dbReference type="CTD" id="79607"/>
    <dbReference type="GeneTree" id="ENSGT00390000010215"/>
    <dbReference type="OMA" id="WGKQNEL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038916">
        <property type="entry name" value="FAM118"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28623">
        <property type="entry name" value="PROTEIN FAM118B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28623:SF1">
        <property type="entry name" value="PROTEIN FAM118B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13289">
        <property type="entry name" value="SIR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="351" mass="39537" checksum="4112F2DCC74EC6A6" modified="2019-09-18" version="1">MASTGSQASDIDKILGFFSDGAPPTKKPRKLLPSLKTKKPRELVLVIGTGISAAVAPQVPALKSWKGLIQALLDAAIDFDLLEDEESKKFQKCLHEDKNLVHVAHDLIQKLSPRTSNVRSTFFKDCLYEVFDDLESKMEDSGKQLLQSVLHLMENGALVLTTNFDNLLELYAADQGKQLESLDLTDEKKVLEWAQEKRKLSVLHIHGVYTNPSGIVLHPAGYQNVLRNTEVMREIQKLYENKSFLFLGCGWTVDDTTFQALFLEAVKHKSDLEHFMLVRRGDVDEFKKLRENMLDKGIKVISYGNDYADLPEYFKRLTCEISTRGRSAGIVREGQLNGSSTAHSEIRDHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H636</accession>
    <name>A0A4W2H636_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Four and a half LIM domains protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FHL3</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with SOX15; the interaction recruits FHL3 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027393570.1">
        <property type="nucleotide sequence ID" value="XM_027537769.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393571.1">
        <property type="nucleotide sequence ID" value="XM_027537770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H636"/>
    <dbReference type="SMR" id="A0A4W2H636"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037240.1">
        <property type="protein sequence ID" value="ENSBIXP00000040960.1"/>
        <property type="gene ID" value="ENSBIXG00000025141.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042337.1">
        <property type="protein sequence ID" value="ENSBIXP00005026238.1"/>
        <property type="gene ID" value="ENSBIXG00005028592.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890110"/>
    <dbReference type="CTD" id="2275"/>
    <dbReference type="GeneTree" id="ENSGT00950000183028"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09423">
        <property type="entry name" value="LIM1_FHL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09427">
        <property type="entry name" value="LIM2_FHL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09346">
        <property type="entry name" value="LIM3_FHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09434">
        <property type="entry name" value="LIM4_FHL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000013">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000030">
        <property type="entry name" value="Four and a half LIM domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000064">
        <property type="entry name" value="Four and a half LIM domains protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000095">
        <property type="entry name" value="four and a half LIM domains protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056807">
        <property type="entry name" value="LIM_FHL1/2/3/5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF5">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25076">
        <property type="entry name" value="LIM_FHL2-3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 17" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="38"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="99"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="219"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="280" mass="31182" checksum="CD125F5352E77B85" modified="2019-09-18" version="1">MSEAFDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HIU3</accession>
    <name>A0A4W2HIU3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA ligase 1</fullName>
            <ecNumber evidence="3">6.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RLIG1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Functions as an RNA ligase, in vitro. The ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate. In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP. Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids. May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2HIU3"/>
    <dbReference type="STRING" id="30522.A0A4W2HIU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034928.1">
        <property type="protein sequence ID" value="ENSBIXP00000039959.1"/>
        <property type="gene ID" value="ENSBIXG00000023969.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008790.1">
        <property type="protein sequence ID" value="ENSBIXP00005030127.1"/>
        <property type="gene ID" value="ENSBIXG00005010182.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044938"/>
    <dbReference type="OMA" id="KQFMYSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041211">
        <property type="entry name" value="RLIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31219">
        <property type="entry name" value="CHROMOSOME 28 C12ORF29 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31219:SF2">
        <property type="entry name" value="RNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17720">
        <property type="entry name" value="RLIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0692">RNA repair</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="325" mass="37430" checksum="1B7871AC3D93A881" modified="2019-09-18" version="1">MRRLGSVQRKMPCVFVTEVKEEPSTKREHQPFKVLATETLSHKALDADIHNAIPTEKVDGTCCYVTNYKGQPYLWARLDRKPNKLAEKRFKNFLHLKQNSKEFFWNVEEDFKPVPECWIPAKEIEQINGNPIPDENGHIPGWVPVEKNSKQHCWHSSVVDYESEIALVLRHHPDDPGLLEISAVPLSDLLEQTLELIGTNINGNPYGLGSKKHPLHLLIPHGAFQIRNLPTLKHSDLLSWFDGCREGKIEGIVWHCNDGCLIKVHRHHLGLCWPIPDTYMNSKPVIINMNLNKYEYAFDAKCLFNHFSKIDNQKFGRLKDIILTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IDQ7</accession>
    <name>A0A4W2IDQ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Syndecan</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SDC1</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain). Interacts with MDK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the syndecan proteoglycan family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411804.1">
        <property type="nucleotide sequence ID" value="XM_027556003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IDQ7"/>
    <dbReference type="SMR" id="A0A4W2IDQ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047980.1">
        <property type="protein sequence ID" value="ENSBIXP00000017453.1"/>
        <property type="gene ID" value="ENSBIXG00000021454.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051461.1">
        <property type="protein sequence ID" value="ENSBIXP00005042163.1"/>
        <property type="gene ID" value="ENSBIXG00005004335.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901529"/>
    <dbReference type="CTD" id="6382"/>
    <dbReference type="GeneTree" id="ENSGT00940000161171"/>
    <dbReference type="OMA" id="VFCFQAV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001050">
        <property type="entry name" value="Syndecan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027789">
        <property type="entry name" value="Syndecan/Neurexin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030479">
        <property type="entry name" value="Syndecan_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10915">
        <property type="entry name" value="SYNDECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10915:SF5">
        <property type="entry name" value="SYNDECAN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01034">
        <property type="entry name" value="Syndecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00964">
        <property type="entry name" value="SYNDECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 15" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 15" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612101" description="Syndecan" evidence="18">
        <location>
            <begin position="23"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Neurexin/syndecan/glycophorin C" evidence="19">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="31"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="141"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="311" mass="32316" checksum="31E163B82F35BBC1" modified="2019-09-18" version="1">MRRAALWLWLCALALRLQPALLHSVAVNMPPEDQDGSGDDSDNFSGSGAGALPDITSSHTPSTWKDLGPVTTTATAPEPTSPDAIAASTTILPTGEQPEGGRAVLLAEVEPGLTAQKEATHPPSETTLHPTTHSVSTARATMAPGPATSHPHRDVQPDHHETSAPTGRGRMEPHRPHVEEGGPPATEKAAEEDPSTQIPVGEGSGEQDFTFDLSGENAAGAAGEPGSRNGAPEDPEATGATGASQGLLDRKEVLGGVIAGGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IHB9</accession>
    <name>A0A4W2IHB9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipid scramblase CLPTM1L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cisplatin resistance-related protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cleft lip and palate transmembrane protein 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CLPTM1L</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Scramblase that mediates the translocation of glucosaminylphosphatidylinositol (alpha-D-GlcN-(1-6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol, GlcN-PI) across the endoplasmic reticulum (ER) membrane, from the cytosolic leaflet to the luminal leaflet of the ER membrane, where it participates in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a lipid glycoconjugate involved in post-translational modification of proteins. Can also translocate 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) (phosphatidylinositol or PI), as well as several other phospholipids (1,2-diacyl-sn-glycero-3-phosphocholine, 1,2-diacyl-sn-glycero-3-phosphoethanolamine), and N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI) in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = 6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71495"/>
            <dbReference type="ChEBI" id="CHEBI:190691"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71491"/>
            <dbReference type="ChEBI" id="CHEBI:57997"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375932.1">
        <property type="nucleotide sequence ID" value="XM_027520131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IHB9"/>
    <dbReference type="STRING" id="30522.A0A4W2IHB9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021115.1">
        <property type="protein sequence ID" value="ENSBIXP00000011634.1"/>
        <property type="gene ID" value="ENSBIXG00000007114.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052537.1">
        <property type="protein sequence ID" value="ENSBIXP00005043472.1"/>
        <property type="gene ID" value="ENSBIXG00005006940.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878946"/>
    <dbReference type="CTD" id="81037"/>
    <dbReference type="GeneTree" id="ENSGT00530000063461"/>
    <dbReference type="OMA" id="TTMWRAF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="538" mass="62117" checksum="117971FE8A7CE038" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2INB1</accession>
    <name>A0A4W2INB1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin-17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SNX17</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels. Binds to NPxY sequences in the cytoplasmic tails of target cargos. Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits. Also required for maintenance of normal cell surface levels of APP and LRP1. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with APP (via cytoplasmic YXNPXY motif). Interacts with KIF1B. Interacts with the C-termini of P-selectin, PTC, LDLR, VLDLR, LRP1 and LRP8. Interacts with KRIT1 (via N-terminus). Interacts with HRAS. Interacts with ITGB1 and ITGB5 (via NPxY motif). Interacts with CCDC22 and CCDC93; the interaction associates SNX17 with the CCC complex. Interacts (via C-terminus) with VPS26C and VPS35L; the interactions are direct and associate SNX17 with the retriever complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411615.1">
        <property type="nucleotide sequence ID" value="XM_027555814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2INB1"/>
    <dbReference type="SMR" id="A0A4W2INB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048314.1">
        <property type="protein sequence ID" value="ENSBIXP00000018148.1"/>
        <property type="gene ID" value="ENSBIXG00000004202.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043931.1">
        <property type="protein sequence ID" value="ENSBIXP00005045646.1"/>
        <property type="gene ID" value="ENSBIXG00005005239.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901451"/>
    <dbReference type="CTD" id="9784"/>
    <dbReference type="GeneTree" id="ENSGT00950000183212"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050750">
        <property type="term" value="F:low-density lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035904">
        <property type="term" value="P:aorta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003279">
        <property type="term" value="P:cardiac septum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060976">
        <property type="term" value="P:coronary vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001822">
        <property type="term" value="P:kidney development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13337">
        <property type="entry name" value="FERM-like_C_SNX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16121">
        <property type="entry name" value="FERM_F1_SNX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06885">
        <property type="entry name" value="PX_SNX17_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.60:FF:000001">
        <property type="entry name" value="Sorting nexin-17 isoform1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000145">
        <property type="entry name" value="Sorting nexin-17 isoform1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000094">
        <property type="entry name" value="Sorting nexin-17 isoform1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000008">
        <property type="entry name" value="Sorting nexin-17 isoform1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048763">
        <property type="entry name" value="SNX17-31_FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048767">
        <property type="entry name" value="SNX17-31_FERM_F2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040842">
        <property type="entry name" value="SNX17/31_FERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037836">
        <property type="entry name" value="SNX17_FERM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028666">
        <property type="entry name" value="SNX17_FERM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF16">
        <property type="entry name" value="SORTING NEXIN-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21273">
        <property type="entry name" value="SNX17-27-31_F1_FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21271">
        <property type="entry name" value="SNX17-31_F2_FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18116">
        <property type="entry name" value="SNX17_FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="15">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="16">
        <location>
            <begin position="115"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="470" mass="52825" checksum="3ABC73220EA919FE" modified="2019-09-18" version="1">MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYAQTVADIERGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGGTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLESPDASRESMVKLSSKLSAVSLRGIGTPGTDASASDVHGNFAFEGIGDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IRN1</accession>
    <name>A0A4W2IRN1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inducible T-cell costimulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ICOS</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homodimer; disulfide-linked. Interacts with ICOSLG. Interacts with PIK3R1. Interacts with TBK1; this interaction is critical for the maturation of T follicular regulatory cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027420850.1">
        <property type="nucleotide sequence ID" value="XM_027565049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IRN1"/>
    <dbReference type="SMR" id="A0A4W2IRN1"/>
    <dbReference type="STRING" id="30522.A0A4W2IRN1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045633.1">
        <property type="protein sequence ID" value="ENSBIXP00000012544.1"/>
        <property type="gene ID" value="ENSBIXG00000017606.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056859.1">
        <property type="protein sequence ID" value="ENSBIXP00005046996.1"/>
        <property type="gene ID" value="ENSBIXG00005031595.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906635"/>
    <dbReference type="CTD" id="29851"/>
    <dbReference type="GeneTree" id="ENSGT00390000000801"/>
    <dbReference type="OMA" id="QDKEDCF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002517">
        <property type="term" value="P:T cell tolerance induction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061470">
        <property type="term" value="P:T follicular helper cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000874">
        <property type="entry name" value="Inducible T-cell costimulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039943">
        <property type="entry name" value="ICOS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20904:SF0">
        <property type="entry name" value="INDUCIBLE T-CELL COSTIMULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20904">
        <property type="entry name" value="INDUCIBLE T-CELL COSTIMULATOR ICOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15910">
        <property type="entry name" value="V-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612183" description="Inducible T-cell costimulator" evidence="12">
        <location>
            <begin position="20"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="13">
        <location>
            <begin position="23"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23953" checksum="F288AA99E11B95A6" modified="2019-09-18" version="1">MKSDLRYFFLFCIQVEILAGEFNDSAASEMFIFHNGGVQILCKYPDTVRQFKMQLLKGDNVLCDLTKTKENEDTVSIRNLNVCKFQLSNNSVSFFLYNLDSSYASYYICKLSIFDPPPFQVDILSREYLNIYESELCCQLKFWLPIGCAAFVTVCVFGCVLMYWLTKKKYPTSVHDPNSEYMFMAAVNTAKKPAPTDVTRNLELPGTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQG1</accession>
    <name>A0A4W2BQG1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 236</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF236</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000014219.1">
        <property type="protein sequence ID" value="ENSBIXP00000001356.1"/>
        <property type="gene ID" value="ENSBIXG00000028659.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000573">
        <property type="entry name" value="Putative zinc finger protein 236"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000264">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000301">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000376">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000481">
        <property type="entry name" value="zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001012">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001071">
        <property type="entry name" value="zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002098">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002170">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002376">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002941">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003384">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000308">
        <property type="entry name" value="zinc finger protein 236 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000753">
        <property type="entry name" value="zinc finger protein 236 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002311">
        <property type="entry name" value="zinc finger protein 236 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000226">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000385">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001017">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF21">
        <property type="entry name" value="ZINC FINGER PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="255"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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            <end position="598"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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            <end position="1024"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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        </location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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        </location>
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        <location>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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    </feature>
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<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the H-rev107 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRQ1"/>
    <dbReference type="STRING" id="30522.A0A4W2BRQ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011335.1">
        <property type="protein sequence ID" value="ENSBIXP00000002720.1"/>
        <property type="gene ID" value="ENSBIXG00000009178.1"/>
    </dbReference>
    <dbReference type="OMA" id="GIMFSIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051496">
        <property type="entry name" value="H-rev107_PLA/AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943">
        <property type="entry name" value="HRAS-LIKE SUPPRESSOR - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943:SF31">
        <property type="entry name" value="PHOSPHOLIPASE A AND ACYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="LRAT" evidence="6">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="164" mass="18669" checksum="F5EB8D74FA90AD26" modified="2019-09-18" version="1">MELHSSFQIRRPGTRDHKGLIQSHTALTLTHLLIPLWMWWTLGKISGTGWSSIMSSLADRARVKKERLCDVVGKDPYRVNNKHDDKYNPFPPSKIVQLVEKLVGREFCYHLLHTNCEHFVNELRYGVSRSDQVRDAVTAATILGVGLIALSSGIMFSINKKQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRU8</accession>
    <name>A0A4W2BRU8_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4">Protein Njmu-R1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C19H17orf75</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2BRU8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012987.1">
        <property type="protein sequence ID" value="ENSBIXP00000001900.1"/>
        <property type="gene ID" value="ENSBIXG00000008443.1"/>
    </dbReference>
    <dbReference type="OMA" id="FEDAICP"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099041">
        <property type="term" value="P:vesicle tethering to Golgi"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028280">
        <property type="entry name" value="Njmu-R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14416">
        <property type="entry name" value="PROTEIN NJMU-R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14416:SF2">
        <property type="entry name" value="PROTEIN NJMU-R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15053">
        <property type="entry name" value="Njmu-R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="43616" checksum="011602384D5BBDC9" modified="2019-09-18" version="1">MLPSLQESLDGDEKELESSEEGGSAEEPSSHYCLYSYRGSRLAQQRADSDDGSPGGTNAETPSGDDFSLSLVDTNLPSEVEPELRSFIAKRLSKGAVFEGLGNVAAVELRIPGYRVGCYYCLFQQEKLLPQTASTDSEQKSSEYVVCFLGGSEKGLELFRLELDKYIQGLKNNMNCEESGGENHIQSYLSSWFADVVCPIQRVVHLFQEKLTFLLHAALSYTPVEVKGSDEKTKRDIHRFLSVASLQGLIHEGTMTSLCMAMTEEQQKSVIIDCSSSQPQFHNAGSNRFCEDWMQAFMNGAEGGNPFLFRQVLENFKLKAIQDTNNLKRFIRQAEMNHYALFKCYMFLKNCGSGDILLKIVKVEHEEMPEAKNVVAVLEEFMKEAAAQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSA8</accession>
    <name>A0A4W2BSA8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Methyl-CpG-binding domain protein 4</fullName>
            <ecNumber evidence="11">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 11">Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">Interacts with MLH1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSA8"/>
    <dbReference type="STRING" id="30522.A0A4W2BSA8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010891.1">
        <property type="protein sequence ID" value="ENSBIXP00000002941.1"/>
        <property type="gene ID" value="ENSBIXG00000009370.1"/>
    </dbReference>
    <dbReference type="OMA" id="MTECHKS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000051">
        <property type="entry name" value="Methyl-CpG-binding domain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.890.10:FF:000013">
        <property type="entry name" value="Methyl-CpG-binding domain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017352">
        <property type="entry name" value="MBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
        <property type="entry name" value="MeCP2/MBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074:SF7">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074">
        <property type="entry name" value="METHYL-CPG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038005">
        <property type="entry name" value="Methyl_CpG_bd_MBD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MBD" evidence="13">
        <location>
            <begin position="76"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00055831"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069821"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR038005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="559" mass="63406" checksum="7FFFDB353221BB17" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSC7</accession>
    <name>A0A4W2BSC7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-box transcription factor TBX6</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
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    </comment>
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        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX6"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        </location>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTG2</accession>
    <name>A0A4W2BTG2_BOBOX</name>
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        <submittedName>
            <fullName evidence="6">Arkadia (RNF111) N-terminal like PKA signaling regulator 2N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARK2N</name>
    </gene>
    <organism evidence="5 7">
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        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSBIXT00000009999.1">
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029306">
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    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15303">
        <property type="entry name" value="RNF111_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="86"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        </location>
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    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF15303"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="350" mass="37444" checksum="39A6670222717C4E" modified="2019-09-18" version="1">MKMEEAVGKVEELIESEVPPKTSEQETAKEEDGSVELESQVPKDGVVDSTVLSSMPCLLMELRRDSSESQLASTESDKPTTGRVYESDSSNHCMLSPSSSGHLADSDTLSSAEENEPSQAETAVEGDPSGVSGATVGRKSRRSRSESETSTMAAKKNRQSSDKQNGRVAKVKGHRSQKHKERIRLLRQKREAAARKKYNLLQDSSTSDSDLTCDSSTSSSDDDEEVSGSSKTITAEIPDGPPVVAHYDMSDTGSEPEVVNVDNLLAAAVVQEHSNSVGGQDTGATWRTSGLLEELNAEAGHLDPGFLASEKTSAGNAPLNEEINIASSDSEVEIVGVQEHARSRVAVMKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BW11</accession>
    <name>A0A4W2BW11_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Semaphorin-3B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BW11"/>
    <dbReference type="STRING" id="30522.A0A4W2BW11"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007820.1">
        <property type="protein sequence ID" value="ENSBIXP00000004442.1"/>
        <property type="gene ID" value="ENSBIXG00000010782.1"/>
    </dbReference>
    <dbReference type="OMA" id="ISLHRCA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000008">
        <property type="entry name" value="semaphorin-3B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF37">
        <property type="entry name" value="SEMAPHORIN-3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021364690" description="Semaphorin-3B" evidence="12">
        <location>
            <begin position="25"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="30"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="578"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="707"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="737"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="753" mass="83840" checksum="B147262B41B4685A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BW66</accession>
    <name>A0A4W2BW66_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BW66"/>
    <dbReference type="STRING" id="30522.A0A4W2BW66"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010194.1">
        <property type="protein sequence ID" value="ENSBIXP00000003268.1"/>
        <property type="gene ID" value="ENSBIXG00000009711.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045773">
        <property type="term" value="P:positive regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061003">
        <property type="term" value="P:positive regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098974">
        <property type="term" value="P:postsynaptic actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11960">
        <property type="entry name" value="SH3_Abp1_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000046">
        <property type="entry name" value="Drebrin-like protein isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035717">
        <property type="entry name" value="Drebrin-like_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF12">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="24" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="26" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="27" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="25" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 29" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="20" id="KW-0770">Synapse</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="31">
        <location>
            <begin position="329"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022949"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="503" mass="56103" checksum="107A9F69459F65FE" modified="2019-09-18" version="1">MDRGTWQATVHGVAIVRQDLMSKPLPPLTGPSLLMKETAMTSVWLALETGEGVNDVRKGACANHVSTMAGFLKGAHVTINARAEEDVEPECIMQKVARASGANYTFHKESSRFQDTGPQAPVGSVYQKTNAVSEIKRVGKDSFWAKAEKEEENRRLEEKRRAEEERQQLEQERRERELREAALREQRYQEQGGEAGLQSRKYEQHEVLSRNREEQPAHPREIFKQKERAMSTTSISSPQPGKLKSPFLQKQLTQPDTPISRESPHATSRPRADLREEPVPSIPPCSVQVEEEAVYEEPPEQETFYEEPPAVQQQDASSEPIDHYPGLSGKGLCARALYDYQAADETEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIDRLCLVEASETPIVPLVEAPGTHHRQPHLVRPIQDGPEGAYGPLQHRCVADVKFHLCVLDGLGPPLGLLSPLLAQLCIEPTTEAILLVPGALTMPNHHKAVGRHSGGDWWVVDRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1L3</accession>
    <name>A0A4W2C1L3_BOBOX</name>
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        <recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="450" mass="48544" checksum="E098534DB093251E" modified="2019-09-18" version="1">KWSQSQGHGGRGVCRGEVHLPPGHSKPLSLPDPPVSEPSSLPACCPALEEAAVAPTRPALPLRGHWARAGGGGGGRWGGNWGLVRLAQSGPGLQVRGETGEGVAAGEEKWREGGFGTGAGSPRRTEARGSRGFPHWKLARSGLLRRSLGPESDPARGHLSLTPALTTSGGGAAWKGCLAQHPQREARGRWGPPAAGRRRRGSWRGRPASSQVAGAGLACPSPAPLQQLRRPEGWGAARKEAGSARRIDASQTMTSCGQQPLNVLAVLSLLISAVLSAHFRVCEPYTDHKGRYHFGFHCPRLSDNKTFVLCCHHNNTVFKYCCNETEFQAVMQANLTAGSEGYMHNNYTALLGVWIYGFFVLMLLVLDLLYYSAMNYDICKVYLARWGIHGRWMKQDPRRWGNPARAPRPGQPAPQPQPPPGPLPQAPQAVHTLQRDSHTPPLMTFQSSSA</sequence>
</entry>
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    <accession>A0A4W2C296</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="105" mass="11450" checksum="20A12A36CACFE239" modified="2019-09-18" version="1">MCSLLLVHIAPPIARLHLLSSSSLTKQHQARPPECISRLPPLSPKLQPRLPDFPSTPCQPCAEGFQPLVPAFLPILPLQVNATPATCPLTFSTRVLLPVYTSYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2Q1</accession>
    <name>A0A4W2C2Q1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subunit">
        <text evidence="5">Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
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        <property type="gene ID" value="ENSBIXG00000001270.1"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin lambda variable 2-8"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021246548" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="212" mass="23060" checksum="3C953A8AC349A80C" modified="2019-09-18" version="1">MAWSPLLLTLVTLCTGSWAQAVLTQPPSVSGSLGQTVTISCTGSSNNIGILGVSWYQQIPGSAPRTLIYNSNKRPSGVPDRFSGTKSGNTGTLTIASLQAEDEADYYCASADLSLTSPTVLQARREVRQKPALLSESGLWGQKHPLQRQPLHLMFGLRLCSVTPRNLVQKNLFTSFLIHPFSSFPLQQNMSDFLNLSRNPQCQGQVPWGQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C641</accession>
    <name>A0A4W2C641_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Archaelysin family metallopeptidase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AMZ1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable zinc metalloprotease.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C641"/>
    <dbReference type="STRING" id="30522.A0A4W2C641"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043539.1">
        <property type="protein sequence ID" value="ENSBIXP00000008252.1"/>
        <property type="gene ID" value="ENSBIXG00000001603.1"/>
    </dbReference>
    <dbReference type="OMA" id="LAKWEMF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052009">
        <property type="entry name" value="Archaemetzincin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32205:SF4">
        <property type="entry name" value="ARCHAEMETZINCIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32205">
        <property type="entry name" value="ARCHAEMETZINCIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="560"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="581" mass="62309" checksum="D7132E1577E321EF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6R5</accession>
    <name>A0A4W2C6R5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Enoyl-CoA delta isomerase 2</fullName>
            <ecNumber evidence="4">5.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21 22">Delta(3),Delta(2)-enoyl-CoA isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Dodecenoyl-CoA isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Peroxisomal 3,2-trans-enoyl-CoA isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">ECI2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2E)-tetradecenoyl-CoA = (3Z)-tetradecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:29847"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
            <dbReference type="ChEBI" id="CHEBI:61968"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:29849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3E)-nonenoyl-CoA = (2E)-nonenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:46068"/>
            <dbReference type="ChEBI" id="CHEBI:76292"/>
            <dbReference type="ChEBI" id="CHEBI:85655"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:46069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3E)-octenoyl-CoA = (2E)-octenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:49852"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:131962"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:49853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(3E)-tetradecenoyl-CoA = (2E)-tetradecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:47476"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
            <dbReference type="ChEBI" id="CHEBI:87710"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:47477"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3Z)-octenoyl-CoA = (2E)-octenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:46044"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:85640"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45228"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45900"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="ChEBI" id="CHEBI:85489"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:45901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.8" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6R5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012835.1">
        <property type="protein sequence ID" value="ENSBIXP00000001974.1"/>
        <property type="gene ID" value="ENSBIXG00000008518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000013">
        <property type="entry name" value="Enoyl-CoA delta isomerase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000026">
        <property type="entry name" value="Enoyl-CoA delta isomerase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000084">
        <property type="entry name" value="Enoyl-CoA delta isomerase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF1">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ACB" evidence="26">
        <location>
            <begin position="38"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="393" mass="42772" checksum="733056A1F4541715" modified="2019-09-18" version="1">MAGLAGRFARRWFSGLLGSPLQVPALGLHVRGPAMLASQKDFNNAVSQVKLLKEDPGNEVKLKLYALYKQATEGPCNVPKPGMLDFINKTKWDAWNALGSLSKEAARQNYVDLVSRLSASSESPSPEAPAADRKQPESDSLVVTSEDGITTIRLNRPAKKNALTTQMYHDIIAALQAASKDESAITVLTGSGDYYCSGNDLTNFTHLPAGGLEEMARSAAALLRDFVNCFIDFPKPLVAVVNGPAVGISVTILGLFDVVYATDRASFHTPFSHLGQSPEGCSSYTFPKIMGSSKAAEMLLFGKKLTAQEACAQGLVTEVFPDGTFQKEVWARLKAYSKLPPNAMRISKQIIRNREKEKLHAVNAEESSVLRERWQSDECMNAIASFLSRKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBX4</accession>
    <name>A0A4W2CBX4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBX4"/>
    <dbReference type="STRING" id="30522.A0A4W2CBX4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017197.1">
        <property type="protein sequence ID" value="ENSBIXP00000009121.1"/>
        <property type="gene ID" value="ENSBIXG00000014835.1"/>
    </dbReference>
    <dbReference type="OMA" id="PGHRMFL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="152" mass="16719" checksum="2EC823C0AA6718E1" modified="2019-09-18" version="1">IKRKFVTDGIFKGELNESFTEELAEVRSMLHQPGQKSLSWPPGHRMFLVRRAGGFRELTAVVQKKFGFPDGRVELYAEKGATGGLQGVLGVKGKSMLPWDPAGKTGPKKPLPEDVSIVEAKDETRPPPLSLNRGSFPSPEDRPNPRDPTQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CC31</accession>
    <name>A0A4W2CC31_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calmodulin regulated spectrin associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CAMSAP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CC31"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008989.1">
        <property type="protein sequence ID" value="ENSBIXP00000003876.1"/>
        <property type="gene ID" value="ENSBIXG00000010263.1"/>
    </dbReference>
    <dbReference type="OMA" id="SNQRTCV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000001">
        <property type="entry name" value="Calmodulin-regulated spectrin-associated protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF3">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="216"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1423"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="348"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="584"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="649"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="726"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1042"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1254"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="851"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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        <source>
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    <sequence length="1562" mass="171294" checksum="6CDA2F1B8CE14ED2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCQ7</accession>
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        <recommendedName>
            <fullName evidence="8">Inhibitor of Bruton tyrosine kinase</fullName>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="7">Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.</text>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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            <topology evidence="1">Peripheral membrane protein</topology>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1415" mass="157440" checksum="2847B11740ACD7BA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
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                <consortium name="Ensembl"/>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="336" mass="37948" checksum="7A358844A85530A9" modified="2019-09-18" version="1">MGIPDHLTCLLRNLYTGQEATVRTGHGTTDWLQIGKGVHQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRSINNLRYADDTTLMAESEEELKSLLMKVKEESETVGLKLNIQKTKIMASGLITSWEIDGQTVATVSDFIFLGSKITADGDCGHEIKRRLLLGRKVMTNLDSTFKSRDITLPTKVHLVKAMVFPVVMCGCESWTVKKAERQRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGCSLEGLMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGKRRRGRQRMRWLDGITDSMDVSLSELRELVMDREAWCAVIHGVAKSQTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CG90</accession>
    <name>A0A4W2CG90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vasopressin V1b receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">AVPR1B</name>
        <name evidence="15" type="synonym">LOC113888834</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027420108.1">
        <property type="nucleotide sequence ID" value="XM_027564307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CG90"/>
    <dbReference type="SMR" id="A0A4W2CG90"/>
    <dbReference type="STRING" id="30522.A0A4W2CG90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045419.1">
        <property type="protein sequence ID" value="ENSBIXP00000012065.1"/>
        <property type="gene ID" value="ENSBIXG00000002839.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006051.1">
        <property type="protein sequence ID" value="ENSBIXP00005031019.1"/>
        <property type="gene ID" value="ENSBIXG00005011239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033007.1">
        <property type="protein sequence ID" value="ENSBIXP00005040412.1"/>
        <property type="gene ID" value="ENSBIXG00005023064.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906331"/>
    <dbReference type="CTD" id="553"/>
    <dbReference type="GeneTree" id="ENSGT01050000244882"/>
    <dbReference type="OMA" id="FNSHLWP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005000">
        <property type="term" value="F:vasopressin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045907">
        <property type="term" value="P:positive regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001992">
        <property type="term" value="P:regulation of systemic arterial blood pressure by vasopressin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15386">
        <property type="entry name" value="7tmA_V1bR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000094">
        <property type="entry name" value="Vasopressin V1a receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015076">
        <property type="entry name" value="V1R_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001817">
        <property type="entry name" value="Vasoprsn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000628">
        <property type="entry name" value="Vprs_rcpt_V1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF18">
        <property type="entry name" value="VASOPRESSIN V1B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00896">
        <property type="entry name" value="VASOPRESSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00897">
        <property type="entry name" value="VASOPRSNV1BR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01164">
        <property type="entry name" value="DUF1856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="51"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU046427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="422" mass="46130" checksum="57F6AC8555515578" modified="2019-09-18" version="1">MDSRPPWTAAPTPGSTLSAANATTPWLGRDEELAKVEIGVLATVLALATGGNLTVLLTVGQPVRKRSRMQVFVLHLALTDLGVALFQVLPQLLWDITYRFRGPDPLCRAVKYLQVLSMFASTYMLLAMTLDRYLAVCHPLRSLQQPSRSTYPLIAAPWLLAAVLSLPQVFIFSIREVIQGSGVLDCWADFRFPWGPRAYITWTTLAIFILPVAMLTACYGLICHEICRNLKVKTEAGQAEGGSWGTGNRPSARGPVAAPRGLPSRVSSVSAISRAKIRTVKMTFVIVLAYIACWAPFFSVQMWSVWDENAPDEDSTNVAFTISMLLGNLSSCCNPWIYMGFNSHLWLHALRRLACCGGPGPRMRRRLSNGSLSSRHATLLTRSSGPPARGLSPGLSRRPGPKDSLRGAEQVEGDAATETSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLS4</accession>
    <name>A0A4W2CLS4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC113904831</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLS4"/>
    <dbReference type="STRING" id="30522.A0A4W2CLS4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022906.1">
        <property type="protein sequence ID" value="ENSBIXP00000012716.1"/>
        <property type="gene ID" value="ENSBIXG00000017752.1"/>
    </dbReference>
    <dbReference type="OMA" id="AINCFMG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15223">
        <property type="entry name" value="7tmA_OR56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF157">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="47"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="35768" checksum="7F99FDAF6012B7DF" modified="2019-09-18" version="1">MDTTLSITNGSRFQVSEFVLMGFPGIHSWQHWLSLPLALLYLSALGANLLILITIHHESTLHEPMYYLLGILAVVDIGLATTIMPKILAIFWFNAKTISLTECFAQLYAINCFMGMESGIFLCMAVDRYVAICYPLQYSSIVTETFVIKVTLSMMLRNGLLTIPVPVLAAQRHYCSRNEIDHCLCSNLGVTSLACDDITINRFYHLALAWFVVGSDMGLVFASYMLIIRSVLRLNSAEATSKALSTCSSHLTLILFFYTAVIVVSVTHLAGRQFPIIPVLLNVLHSVIPPALNPMVYALRTQELRVGFQRLIGLGEKMPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMD3</accession>
    <name>A0A4W2CMD3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMD3"/>
    <dbReference type="STRING" id="30522.A0A4W2CMD3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023114.1">
        <property type="protein sequence ID" value="ENSBIXP00000012935.1"/>
        <property type="gene ID" value="ENSBIXG00000017938.1"/>
    </dbReference>
    <dbReference type="OMA" id="SKCANIK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39879" checksum="F4E87AE59FE2BFA5" modified="2019-09-18" version="1">MQRQLKEFTSSQEESMFTQTIEKLGLSITILAKGGSWGISMEAGMDQSMHSESKKIQQSHSTHSYFCSTKFSYIPLASCIFPIDQLQLSNAALQELKSIEDLLGQSEDPDKLFLLRHRAEAFFQRFGSHANQGPLHLGGIYWWKAISEGFQKEQLAEVKQQAAEALDSYIRGSSSGFGVNIGTGLGVSDSHSKKASQRKSSKNLQTKAKLSVAQTGGPPEANGLSDWKEGLVDNNQTWCVIDRGLQLVPVWDIVLSGHRSDFKDPLKVANCLKDSYTALNEFTAQIQEGEELLSIKKEAGIFLEDVKSWKVLNPEEQLESLRNFMQRLSQKTEMFPMKRKSPTNHTIMKRKRRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CP00</accession>
    <name>A0A4W2CP00_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sorcin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SRI</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027395124.1">
        <property type="nucleotide sequence ID" value="XM_027539323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CP00"/>
    <dbReference type="SMR" id="A0A4W2CP00"/>
    <dbReference type="STRING" id="30522.A0A4W2CP00"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026027.1">
        <property type="protein sequence ID" value="ENSBIXP00000014874.1"/>
        <property type="gene ID" value="ENSBIXG00000019482.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022215.1">
        <property type="protein sequence ID" value="ENSBIXP00005012711.1"/>
        <property type="gene ID" value="ENSBIXG00005016923.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891367"/>
    <dbReference type="CTD" id="6717"/>
    <dbReference type="GeneTree" id="ENSGT00940000153979"/>
    <dbReference type="OMA" id="LNQFIYC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140311">
        <property type="term" value="F:protein sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055118">
        <property type="term" value="P:negative regulation of cardiac muscle contraction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010459">
        <property type="term" value="P:negative regulation of heart rate"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051281">
        <property type="term" value="P:positive regulation of release of sequestered calcium ion into cytosol"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086004">
        <property type="term" value="P:regulation of cardiac muscle cell contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901844">
        <property type="term" value="P:regulation of cell communication by electrical coupling involved in cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061178">
        <property type="term" value="P:regulation of insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901077">
        <property type="term" value="P:regulation of relaxation of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010880">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16187">
        <property type="entry name" value="EFh_PEF_sorcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000087">
        <property type="entry name" value="Sorcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF7">
        <property type="entry name" value="SORCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="198" mass="21636" checksum="428E41CDF4A0A2A3" modified="2019-09-18" version="1">MAYPGHPGAGGGYYAGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPFNLETCRLMVSMLDRDMSGTMGFNEFKELWAVLNGWRQHFISFDSDRSGTVDPQELQKALTTMGFRLSPQAVNSIAKRYSTNGKITFDDYIACCVKLRALTDSFRRRDTAQQGVVNFPYDDFIQCVMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQA6</accession>
    <name>A0A4W2CQA6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Collagen alpha-1(I) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="388" mass="42167" checksum="228131BE7E77B83B" modified="2019-09-18" version="1">MLPELATTSAHQGHQLPATCLRTDPAQGGPCPEPQLGSTSHLPGHKPLRPRPMMEATGLEGLESTSSPCPGSTGTGLSWDNGTRHNATFPEPLPALYVLLPVVYSVICAVGLVGNAAVICVILRAPKMKTVTHVFILNLAIADGLFTLVLPTNIAEHLLQRWPFGEVLCKLVLAIDHCNIFSSVYFLAAMSIDRYLVVLATARSRRMPRRTVHRAKVASLCVWLGVTVAVLPFLTFAGVYNNELQVTSCGLSFPRPERAWFQASRIYTLVLGFVVPMCTLCVLYADLLRRLRALRLHSGAKALGKAKQKVSLLVLAVLAVGLLCWTPFHLASIVALTTDLPQTPLVIIVSYVVTSLSYTSSCLNPFLYAFLDHSFRKSLRTACRCQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTU0</accession>
    <name>A0A4W2CTU0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulfiredoxin-1</fullName>
            <ecNumber evidence="2">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SRXN1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfiredoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTU0"/>
    <dbReference type="SMR" id="A0A4W2CTU0"/>
    <dbReference type="STRING" id="30522.A0A4W2CTU0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028818.1">
        <property type="protein sequence ID" value="ENSBIXP00000016730.1"/>
        <property type="gene ID" value="ENSBIXG00000003917.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035547.1">
        <property type="protein sequence ID" value="ENSBIXP00005021598.1"/>
        <property type="gene ID" value="ENSBIXG00005024577.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007832"/>
    <dbReference type="OMA" id="HHIQQIP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16395">
        <property type="entry name" value="Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1530.10:FF:000001">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="13">
        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="292" mass="30967" checksum="9335C9B9C5BEA5E6" modified="2019-09-18" version="1">QSKGRAAKRRVLPTHPRWARPAPSTLCPRHGPESPRNVRHPRPSHSTTCRLTLSQPARPLPLPGRRAAEGRRRGAPQRRAEAPRASGSRGRAEQRSRRGARWWAGPPWGQGRETLRAARLRPPPPARNGTARPRLKPGGGGAASRDGPGGGGGGMMGLRAGGALSRAGAGRGAPEVPGPSGGAQGGSIHSGCITAVHNVPLSVLIRPLPSVLDQAKVQSLVDTIRENPDSVPPIDVLWIKGAQGGDYFYSFGGCHRYAAYQQLQRETIPAKLVQSTLSDLRVYLGASTPDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW15</accession>
    <name>A0A4W2CW15_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="11">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FKBP1B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Identified in a complex composed of RYR2, FKBP1B, PKA catalytic subunit, PRKAR2A, AKAP6, and the protein phosphatases PP2A and PP1. Interacts directly with RYR2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027411758.1">
        <property type="nucleotide sequence ID" value="XM_027555957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW15"/>
    <dbReference type="SMR" id="A0A4W2CW15"/>
    <dbReference type="STRING" id="30522.A0A4W2CW15"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030216.1">
        <property type="protein sequence ID" value="ENSBIXP00000017610.1"/>
        <property type="gene ID" value="ENSBIXG00000021600.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052175.1">
        <property type="protein sequence ID" value="ENSBIXP00005024021.1"/>
        <property type="gene ID" value="ENSBIXG00005026682.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901507"/>
    <dbReference type="CTD" id="2281"/>
    <dbReference type="GeneTree" id="ENSGT00940000153311"/>
    <dbReference type="OMA" id="EQFDASW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019855">
        <property type="term" value="F:calcium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005528">
        <property type="term" value="F:FK506 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035773">
        <property type="term" value="P:insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050849">
        <property type="term" value="P:negative regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010459">
        <property type="term" value="P:negative regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061179">
        <property type="term" value="P:negative regulation of insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051280">
        <property type="term" value="P:negative regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019227">
        <property type="term" value="P:neuronal action potential propagation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010881">
        <property type="term" value="P:regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006939">
        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042098">
        <property type="term" value="P:T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000008">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF429">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="12">
        <location>
            <begin position="20"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="108" mass="11783" checksum="BAC2A25945F63AC4" modified="2019-09-18" version="1">MGVEIETISPGDGRTFPKKGQTCVVHYTGMLQNGKKFDSSRDRNKPFKFRIGKQEVIKGFEEGAAQMSLGQRAKLTCTPDVAYGATGHPGVIPPNATLIFDVELLNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXU3</accession>
    <name>A0A4W2CXU3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">General transcription factor IIE subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transcription initiation factor IIE subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GTF2E1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80. Interacts with ATF7IP. Interacts with SND1. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXU3"/>
    <dbReference type="STRING" id="30522.A0A4W2CXU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031268.1">
        <property type="protein sequence ID" value="ENSBIXP00000018298.1"/>
        <property type="gene ID" value="ENSBIXG00000022166.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004410.1">
        <property type="protein sequence ID" value="ENSBIXP00005006430.1"/>
        <property type="gene ID" value="ENSBIXG00005011917.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016696"/>
    <dbReference type="OMA" id="DAIKWKV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000087">
        <property type="entry name" value="General transcription factor IIE subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021600">
        <property type="entry name" value="TFIIE_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF8">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11521">
        <property type="entry name" value="TFIIE-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="16">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="330"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="439" mass="49397" checksum="45B0243821FC3FD7" modified="2019-09-18" version="1">MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCQTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDRAATAAGAAGLAGGHHREAWTTKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYNSEEMKEGGIDIDSFQEHEEGHAGPDDNEEVMRALLIHEKKTPSAPAGSVGAAAPVTAANGSDSESETSESDDDSPPRVATVAAHHGEEDEEDDEFEEVADDPVVMVAGRPFSYSEVSQKPELVAQMTPEEKEAYIAMGQRMFEDLFEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CY88</accession>
    <name>A0A4W2CY88_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PHD finger protein 20-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PHF20L1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CY88"/>
    <dbReference type="STRING" id="30522.A0A4W2CY88"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045043.1">
        <property type="protein sequence ID" value="ENSBIXP00000011313.1"/>
        <property type="gene ID" value="ENSBIXG00000005962.1"/>
    </dbReference>
    <dbReference type="OMA" id="QGHMLQL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20104">
        <property type="entry name" value="MBT_PHF20L1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15633">
        <property type="entry name" value="PHD_PHF20L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20454">
        <property type="entry name" value="Tudor_PHF20L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000049">
        <property type="entry name" value="PHD finger protein 20 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
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                <person name="Bickhart D.M."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N-acetyl-beta-D-6-sulfogalactosaminyl-(1-&gt;4)-alpha-L-iduronyl-(1-&gt;3)-N-acetyl-D-6-sulfogalactosamine + H2O = alpha-L-iduronyl-(1-&gt;3)-N-acetyl-D-6-sulfogalactosamine + N-acetyl-D-6-sulfogalactosamine</text>
            <dbReference type="Rhea" id="RHEA:64384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:152567"/>
            <dbReference type="ChEBI" id="CHEBI:152568"/>
            <dbReference type="ChEBI" id="CHEBI:153064"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:64385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N-acetyl-beta-D-galactosaminyl-(1-&gt;4)-beta-D-3-sulfogalactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + H2O = a beta-D-3-sulfogalactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + N-acetyl-beta-D-galactosamine</text>
            <dbReference type="Rhea" id="RHEA:48276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28497"/>
            <dbReference type="ChEBI" id="CHEBI:90163"/>
            <dbReference type="ChEBI" id="CHEBI:90164"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:48277"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a ganglioside GM2 (d18:1(4E)) + H2O = a ganglioside GM3 (d18:1(4E)) + N-acetyl-beta-D-galactosamine</text>
            <dbReference type="Rhea" id="RHEA:47940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28497"/>
            <dbReference type="ChEBI" id="CHEBI:60065"/>
            <dbReference type="ChEBI" id="CHEBI:71502"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ganglioside GM2 + H2O = a ganglioside GM3 + N-acetyl-beta-D-galactosamine</text>
            <dbReference type="Rhea" id="RHEA:47968"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28497"/>
            <dbReference type="ChEBI" id="CHEBI:79210"/>
            <dbReference type="ChEBI" id="CHEBI:79218"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>beta-D-GalNAc-(1-&gt;4)-alpha-L-IdoA-(1-&gt;3)-beta-D-GalNAc-4-sulfate-(1-&gt;4)-alpha-L-IdoA-(1-&gt;3)-D-GalNAc-4-sulfate + H2O = alpha-L-IdoA-(1-&gt;3)-beta-D-GalNAc-4-sulfate-(1-&gt;4)-alpha-L-IdoA-(1-&gt;3)-D-GalNAc-4-sulfate + N-acetyl-D-galactosamine</text>
            <dbReference type="Rhea" id="RHEA:64372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28037"/>
            <dbReference type="ChEBI" id="CHEBI:152565"/>
            <dbReference type="ChEBI" id="CHEBI:152566"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64373"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">There are 3 beta-hexosaminidase isozymes: isozyme A (hexosaminidase A) is a heterodimer composed of one subunit alpha and one subunit beta (chain A and B); isozyme B (hexosaminidase B) is a homodimer of two beta subunits (two chains A and B); isozyme S (hexosaminidase S) is a homodimer of two alpha subunits. The composition of the dimer (isozyme A versus isozyme S) has a significant effect on the substrate specificity of the alpha subunit active site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="19"/>
    <dbReference type="RefSeq" id="XP_027408947.1">
        <property type="nucleotide sequence ID" value="XM_027553146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D332"/>
    <dbReference type="SMR" id="A0A4W2D332"/>
    <dbReference type="STRING" id="30522.A0A4W2D332"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034200.1">
        <property type="protein sequence ID" value="ENSBIXP00000020230.1"/>
        <property type="gene ID" value="ENSBIXG00000023578.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056035.1">
        <property type="protein sequence ID" value="ENSBIXP00005046300.1"/>
        <property type="gene ID" value="ENSBIXG00005031188.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899827"/>
    <dbReference type="CTD" id="3073"/>
    <dbReference type="GeneTree" id="ENSGT00390000008107"/>
    <dbReference type="OMA" id="KMWPRAA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042582">
        <property type="term" value="C:azurophil granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905379">
        <property type="term" value="C:beta-N-acetylhexosaminidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007628">
        <property type="term" value="P:adult walking behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048667">
        <property type="term" value="P:cell morphogenesis involved in neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030209">
        <property type="term" value="P:dermatan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050884">
        <property type="term" value="P:neuromuscular process controlling posture"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000049">
        <property type="entry name" value="Beta-hexosaminidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF39">
        <property type="entry name" value="BETA-HEXOSAMINIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 19" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610663" description="Beta-hexosaminidase" evidence="22">
        <location>
            <begin position="23"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="24">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="23">
        <location>
            <begin position="167"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="20">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="58"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="277"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="529" mass="60353" checksum="878641B502FCD3A9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3A7</accession>
    <name>A0A4W2D3A7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zyxin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3A7"/>
    <dbReference type="STRING" id="30522.A0A4W2D3A7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034325.1">
        <property type="protein sequence ID" value="ENSBIXP00000020317.1"/>
        <property type="gene ID" value="ENSBIXG00000004700.1"/>
    </dbReference>
    <dbReference type="OMA" id="YAPKCSV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09353">
        <property type="entry name" value="LIM2_Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09435">
        <property type="entry name" value="LIM3_Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000027">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000057">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000076">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212:SF1">
        <property type="entry name" value="ZYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212">
        <property type="entry name" value="ZYXIN/TRIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 16" id="KW-0440">LIM domain</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="424"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="484"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="545"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="82"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="207"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="253"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="612" mass="65633" checksum="10F1EC8EEAB286DF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3F3</accession>
    <name>A0A4W2D3F3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Myosin IF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MYO1F</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027403299.1">
        <property type="nucleotide sequence ID" value="XM_027547498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3F3"/>
    <dbReference type="SMR" id="A0A4W2D3F3"/>
    <dbReference type="STRING" id="30522.A0A4W2D3F3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048614.1">
        <property type="protein sequence ID" value="ENSBIXP00000018728.1"/>
        <property type="gene ID" value="ENSBIXG00000004375.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008167.1">
        <property type="protein sequence ID" value="ENSBIXP00005004526.1"/>
        <property type="gene ID" value="ENSBIXG00005000747.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895817"/>
    <dbReference type="CTD" id="4542"/>
    <dbReference type="GeneTree" id="ENSGT00940000158870"/>
    <dbReference type="OMA" id="PPEEYQM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11827">
        <property type="entry name" value="SH3_MyoIe_If_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000010">
        <property type="entry name" value="Unconventional myosin-Ie"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035507">
        <property type="entry name" value="Ie/If_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF663">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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</entry>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAS10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D6I3"/>
    <dbReference type="SMR" id="A0A4W2D6I3"/>
    <dbReference type="STRING" id="30522.A0A4W2D6I3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033620.1">
        <property type="protein sequence ID" value="ENSBIXP00000019825.1"/>
        <property type="gene ID" value="ENSBIXG00000004610.1"/>
    </dbReference>
    <dbReference type="OMA" id="PVHYNET"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="123"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="252"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="315" mass="35766" checksum="A81FB585EE7449BA" modified="2019-09-18" version="1">MAAPEVLESDLPNAVALLKNLQEQVMAVTAQVQTLTKKVQAKAYPTEKGLSLLEVKDQLLLMYLMDLSHLILDKASGGSLQGHPAVLRLVEIRTVLEKLRPLDQKLKYQIDKLVKTAVTGSLSENDPLRFKPHPSNMMSKLSSEDEEEDEAEEGQSGASGKKSGKGTAKKYVPPRLVPVHYDETEAEREKKRLERAKRRALSSSVIRELKEQYSDAPEEIRDARHPHVTRQSQEDQHRINYEESMMVRLSVSKREKGRRKRANVMSSQLHSLTHFSDISALTGGTPHLDEDQNPTKKRKKIPKKGRKKKGFRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D793</accession>
    <name>A0A4W2D793_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D793"/>
    <dbReference type="SMR" id="A0A4W2D793"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034015.1">
        <property type="protein sequence ID" value="ENSBIXP00000020092.1"/>
        <property type="gene ID" value="ENSBIXG00000002641.1"/>
    </dbReference>
    <dbReference type="OMA" id="IPISEHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF101">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021470005" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="147" mass="15824" checksum="9DE101EDC3F62AFF" modified="2019-09-18" version="1">MGCSPVCCVALCLLAAGLVDSGVTQTPRYLIKARGQRGTLGCSPVSGHLSVSWYQQALGQSPEFLIQYYRQEVRGEAQLPDRFSGKQFSDFHSELNLSSLELTDSAVYLCASSQDTALHDQLPLGQKHSCPSSGSGCEGDCLPVRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D870</accession>
    <name>A0A4W2D870_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">SPARC related modular calcium binding 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SMOC1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408082.1">
        <property type="nucleotide sequence ID" value="XM_027552281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D870"/>
    <dbReference type="STRING" id="30522.A0A4W2D870"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034502.1">
        <property type="protein sequence ID" value="ENSBIXP00000020428.1"/>
        <property type="gene ID" value="ENSBIXG00000023747.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899045"/>
    <dbReference type="CTD" id="64093"/>
    <dbReference type="OMA" id="GERDNHC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16240">
        <property type="entry name" value="EFh_SPARC_SMOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000076">
        <property type="entry name" value="SPARC-related modular calcium binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000012">
        <property type="entry name" value="SPARC-related modular calcium binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000004">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000003">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037639">
        <property type="entry name" value="SMOC1_EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF13">
        <property type="entry name" value="SPARC-RELATED MODULAR CALCIUM-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16597">
        <property type="entry name" value="Thyroglob_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021202272" evidence="13">
        <location>
            <begin position="25"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="15">
        <location>
            <begin position="37"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="14">
        <location>
            <begin position="92"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="14">
        <location>
            <begin position="224"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="149"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="129"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="262"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="435" mass="48202" checksum="21F96B3C115079BE" modified="2019-09-18" version="1">MLPARCARLLTPHLLLALVQLSPAHDHRTTGPRFLISDRDPQCNLHCSRTQPKPVCASDGRSYESMCEYQRAKCRDPTLAVAHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECTEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGSVTDKPASQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNSEKVHSCDQERQSALEEARQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPLPGTSTRYVMPSCESDARAKSAEVEDPFKDRELPGCPEGKKLEFITSLLDALTTDMVQAINSAAPTGGGRFSEPDPSHTLEERVVHWYFSQLDSNSSSDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEVGRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8L5</accession>
    <name>A0A4W2D8L5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-alanine-activating enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-CoA synthetase family member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">AASDH</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8L5"/>
    <dbReference type="STRING" id="30522.A0A4W2D8L5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037397.1">
        <property type="protein sequence ID" value="ENSBIXP00000022328.1"/>
        <property type="gene ID" value="ENSBIXG00000025222.1"/>
    </dbReference>
    <dbReference type="OMA" id="NGNVICC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17654">
        <property type="entry name" value="A_NRPS_acs4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000027">
        <property type="entry name" value="AASDH isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000014">
        <property type="entry name" value="acyl-CoA synthetase family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001301">
        <property type="entry name" value="Beta-alanine-activating enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.10">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048005">
        <property type="entry name" value="AASDH_AMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052091">
        <property type="entry name" value="Beta-ala_Activ/Resist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394:SF1">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="Beta-prop_ACSF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="580"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1132" mass="126940" checksum="B12EA314C46DD5EA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA55</accession>
    <name>A0A4W2DA55_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Histone-lysine N-methyltransferase 2C</fullName>
            <ecNumber evidence="22">2.1.1.364</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">KMT2C</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place. Likely plays a redundant role with KMT2D in enriching H3K4me1 mark on primed and active enhancer elements.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60264"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.364"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:60265"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Component of the MLL3 complex (also named ASCOM complex), at least composed of catalytic subunit KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin. Forms a core complex with the evolutionary conserved subcomplex WRAD composed of WDR5, RBBP5, ASH2L/ASH2 and DPY30 subunits; WRAD differentially stimulates the methyltransferase activity. Interacts (via WIN motif) with WDR5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.364" evidence="22"/>
    <dbReference type="STRING" id="30522.A0A4W2DA55"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049764.1">
        <property type="protein sequence ID" value="ENSBIXP00000021058.1"/>
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        <location>
            <begin position="2676"/>
            <end position="2691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="2824"/>
            <end position="2843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="2848"/>
            <end position="2859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="2945"/>
            <end position="2958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="3025"/>
            <end position="3038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="29">
        <location>
            <begin position="3216"/>
            <end position="3232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="3233"/>
            <end position="3243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="3289"/>
            <end position="3313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="3346"/>
            <end position="3363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="3406"/>
            <end position="3420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="3472"/>
            <end position="3483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="3484"/>
            <end position="3503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="3551"/>
            <end position="3563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="3579"/>
            <end position="3595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="3694"/>
            <end position="3704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="3807"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="4046"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="4057"/>
            <end position="4067"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00058707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00065668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00072631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="4969" mass="542356" checksum="98273FABDC6ECC92" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA88</accession>
    <name>A0A4W2DA88_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiosis-specific nuclear structural protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MNS1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. May play a role in the control of meiotic division and germ cell differentiation through regulation of pairing and recombination during meiosis. Required for sperm flagella assembly. May play a role in the assembly and function of the outer dynein arm-docking complex (ODA-DC). ODA-DC mediates outer dynein arms (ODA) binding onto the axonemal doublet microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Able to form oligomers. Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with ODAD1. Interacts with BBOF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA88"/>
    <dbReference type="STRING" id="30522.A0A4W2DA88"/>
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        <property type="protein sequence ID" value="ENSBIXP00000015655.1"/>
        <property type="gene ID" value="ENSBIXG00000003642.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026504">
        <property type="entry name" value="MNS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19265">
        <property type="entry name" value="MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19265:SF0">
        <property type="entry name" value="MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="17">
        <location>
            <begin position="146"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="277"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="526" mass="64363" checksum="3459D2B126EC88DC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAD9</accession>
    <name>A0A4W2DAD9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Coagulation factor V</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Activated protein C cofactor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Factor Va, the activated form of factor V, is composed of a heavy chain and a light chain, non-covalently bound. The interaction between the two chains is calcium-dependent. Forms heterodimer with SERPINA5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000047123.1">
        <property type="protein sequence ID" value="ENSBIXP00000015707.1"/>
        <property type="gene ID" value="ENSBIXG00000020103.1"/>
    </dbReference>
    <dbReference type="OMA" id="YQSNIMS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031091">
        <property type="term" value="C:platelet alpha granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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</entry>
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        <alternativeName>
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        <alternativeName>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="44 45" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="44" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-oxo-3-sulfanylpropanoate + L-kynurenine = kynurenate + L-cysteine + H2O</text>
            <dbReference type="Rhea" id="RHEA:69104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57678"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:69105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-oxoadipate + L-kynurenine = L-2-aminoadipate + kynurenate + H2O</text>
            <dbReference type="Rhea" id="RHEA:70047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70048"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-oxobutanoate + L-kynurenine = (2S)-2-aminobutanoate + kynurenate + H2O</text>
            <dbReference type="Rhea" id="RHEA:66044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2-oxohexanoate + L-kynurenine = L-2-aminohexanoate + kynurenate + H2O</text>
            <dbReference type="Rhea" id="RHEA:66060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35177"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="ChEBI" id="CHEBI:58455"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:66061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-oxopentanoate + L-kynurenine = L-2-aminopentanoate + kynurenate + H2O</text>
            <dbReference type="Rhea" id="RHEA:66076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28644"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58441"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3-phenylpyruvate + L-kynurenine = kynurenate + L-phenylalanine + H2O</text>
            <dbReference type="Rhea" id="RHEA:66092"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:66093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>4-methyl-2-oxopentanoate + L-kynurenine = kynurenate + L-leucine + H2O</text>
            <dbReference type="Rhea" id="RHEA:66068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:66069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4-methylsulfanyl-2-oxobutanoate + L-kynurenine = kynurenate + L-methionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:69096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:69097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-2-aminoadipate + glyoxylate = 2-oxoadipate + glycine</text>
            <dbReference type="Rhea" id="RHEA:69112"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:69113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-kynurenine + 2-oxoglutarate = kynurenate + L-glutamate + H2O</text>
            <dbReference type="Rhea" id="RHEA:65560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="EC" id="2.6.1.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:65561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-kynurenine + glyoxylate = kynurenate + glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:65896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
            <dbReference type="EC" id="2.6.1.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:65897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-leucine + glyoxylate = 4-methyl-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:69128"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tryptophan + glyoxylate = indole-3-pyruvate + glycine</text>
            <dbReference type="Rhea" id="RHEA:69124"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosine + glyoxylate = 3-(4-hydroxyphenyl)pyruvate + glycine</text>
            <dbReference type="Rhea" id="RHEA:69116"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>glycine + 2-oxoglutarate = glyoxylate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14089"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="EC" id="2.6.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>glyoxylate + L-methionine = 4-methylsulfanyl-2-oxobutanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:22884"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="EC" id="2.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>glyoxylate + L-phenylalanine = 3-phenylpyruvate + glycine</text>
            <dbReference type="Rhea" id="RHEA:69120"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>indole-3-pyruvate + L-kynurenine = kynurenate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:66052"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66053"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="27">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.39" evidence="29"/>
    <dbReference type="EC" id="2.6.1.4" evidence="28"/>
    <dbReference type="EC" id="2.6.1.63" evidence="4"/>
    <dbReference type="EC" id="2.6.1.7" evidence="3"/>
    <dbReference type="EC" id="2.6.1.73" evidence="30"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCM0"/>
    <dbReference type="STRING" id="30522.A0A4W2DCM0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036842.1">
        <property type="protein sequence ID" value="ENSBIXP00000021938.1"/>
        <property type="gene ID" value="ENSBIXG00000005084.1"/>
    </dbReference>
    <dbReference type="OMA" id="TQGYPPL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047958">
        <property type="term" value="F:glycine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047315">
        <property type="term" value="F:kynurenine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050094">
        <property type="term" value="F:methionine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000071">
        <property type="entry name" value="Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000166">
        <property type="entry name" value="Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="43">
        <location>
            <begin position="65"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="42">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="42">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="42">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00056991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00060610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00066546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00067027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00067059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00074091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00075068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00080697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00080916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00081438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00082040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00082705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00082796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00083236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00083286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00083735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="429" mass="48644" checksum="1DE8C0B8491E1CCF" modified="2019-09-18" version="1">MNYTRFITAKSAARKPYSIRVMYEKLDKAPKSVISLATGVPNPNMFPFKTAVITIENGKAIQFDEELMKKALQYSQSAGLPELLSWLKQLQVKLHNPPTIHYAPSQGQMDLCVTCGSLEGLYKVFEMIVNPGDNILVNEPVYSGTIHALQPLGCNMINVSSDEHGIIPDSLREILSKWKPEDSKDPEKNSPKCLYTVPNGNNPSGNSLTAERKREIYELARKYDFLIIEDDPYYFMQFNKPWAPTFLSMDVDGRVIRADSFSKVLSSGLRVGFITGPKPLIERIVLHIQVSTLHTSTFTQLLVSQLLHQWGEEGFLTHIDRVTDFYRKQRDALLAAADKWLSGLAEWQVPTAGMFLWVKIKGIHDVRKLIEEKAVKNEIFMLPGYGFYIDSSAPCPYFRASFSSASPEQMDMVSYQFIFCVHFVSYNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEB1</accession>
    <name>A0A4W2DEB1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-sarcoglycan</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD. The association between SGCB and SGCG is particularly strong while SGCA is loosely associated with the other sarcoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <location evidence="3">Sarcolemma</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sarcoglycan beta/delta/gamma/zeta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEB1"/>
    <dbReference type="SMR" id="A0A4W2DEB1"/>
    <dbReference type="STRING" id="30522.A0A4W2DEB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050583.1">
        <property type="protein sequence ID" value="ENSBIXP00000022645.1"/>
        <property type="gene ID" value="ENSBIXG00000007652.1"/>
    </dbReference>
    <dbReference type="OMA" id="KGVQGME"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006875">
        <property type="entry name" value="Sarcoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027659">
        <property type="entry name" value="Sgcb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21142:SF2">
        <property type="entry name" value="BETA-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21142">
        <property type="entry name" value="SARCOGLYCANS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04790">
        <property type="entry name" value="Sarcoglycan_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="317" mass="34734" checksum="9DE3D0DFD15E0030" modified="2019-09-18" version="1">MAAAAAATEQQSSNGPVKKSMREKAVERRNVNKEHNSNFKAGYIPIDEDRLHKTGLRGRKGNLAICVIVLLFILAVINLIITLVIWAVIRIGPNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVGKNKTSITSDTGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVGGRAIVRGNEGVFIMGKTIQFHMGGNMELKAENSIILNGTVMVSTNRLPSSSSGDQFGGDDWVRYKLCMCADGTLFRVQVTGQNMGCQTSDNPCGDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIB0</accession>
    <name>A0A4W2DIB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIB0"/>
    <dbReference type="STRING" id="30522.A0A4W2DIB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052566.1">
        <property type="protein sequence ID" value="ENSBIXP00000025950.1"/>
        <property type="gene ID" value="ENSBIXG00000006767.1"/>
    </dbReference>
    <dbReference type="OMA" id="MYQVSKG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045031">
        <property type="term" value="F:G protein-coupled ATP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000283">
        <property type="entry name" value="Purinergic receptor P2Y11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF46">
        <property type="entry name" value="P2Y PURINOCEPTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="12"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="338" mass="36414" checksum="6AC910E5E5B2C855" modified="2019-09-18" version="1">MLVVEFLVALAGNSLALYRFCSREQHLWPPAMIFSAQLAVSDLLYALTLPPLAAYIYPPKHWRYGEAACRLERFLFTCNLLGSVIFITCISLNRYMGIVHPFFTHSQLRPKHAWAVSAAGWVLAVLLAAPTLSFSHLSESQRPGQCSVANLGACTKCLGTAGDSQLEAYRVYSLALATLGCGLPLLLTLAAYGALGRAVQHSHGMTAANKLQVTVLVASGVALYASSYVPYYITAVLNVYARLRWLALCPGFTSEAAAKKALAQRTYLAHQVTRGLVPLAICIHPLLYMAVVPSLDCCHQCCGQGQTPKSTTCSSQALPLNVTATPQTSGLQSHELSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJI8</accession>
    <name>A0A4W2DJI8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the krueppel C2H2-type zinc-finger protein family. ZFX/ZFY subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJI8"/>
    <dbReference type="STRING" id="30522.A0A4W2DJI8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053255.1">
        <property type="protein sequence ID" value="ENSBIXP00000026516.1"/>
        <property type="gene ID" value="ENSBIXG00000029889.1"/>
    </dbReference>
    <dbReference type="OMA" id="HQESKSH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000054">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000209">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000170">
        <property type="entry name" value="Zinc finger protein 711 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000607">
        <property type="entry name" value="zinc finger X-chromosomal protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000461">
        <property type="entry name" value="Zinc finger X-chromosomal protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006794">
        <property type="entry name" value="Transcrp_activ_Zfx/Zfy-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04704">
        <property type="entry name" value="Zfx_Zfy_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="421"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="515"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="629"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="686"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="743"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="801" mass="90386" checksum="CE24004F0ABA29C6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKL7</accession>
    <name>A0A4W2DKL7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033067.1">
        <property type="protein sequence ID" value="ENSBIXP00000019464.1"/>
        <property type="gene ID" value="ENSBIXG00000004533.1"/>
    </dbReference>
    <dbReference type="OMA" id="HTQTSEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000878">
        <property type="entry name" value="T cell receptor alpha variable 38-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF45">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="112" mass="12963" checksum="C16B968230D6BCFB" modified="2019-09-18" version="1">AQKVTQDQPVISSQVGQSVTLNCWYETSYWSYYNLFWYKQLPNGQMTYLIEQYSEYGNARNGHYSVNFQKADKSISLIISSLQLEDSAKYFCALWELTVLEVIGKAVQKPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMI5</accession>
    <name>A0A4W2DMI5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger FYVE domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZFYVE1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with RAB18 (in GTP-bound form). Interacts with BSCL2 in a RAB18-dependent manner. Interacts with ZW10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">Golgi stack</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMI5"/>
    <dbReference type="SMR" id="A0A4W2DMI5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034005.1">
        <property type="protein sequence ID" value="ENSBIXP00000020099.1"/>
        <property type="gene ID" value="ENSBIXG00000023473.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19819">
        <property type="entry name" value="Bbox1_ZFYVE1_rpt1"/>
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    </dbReference>
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        <property type="entry name" value="Bbox1_ZFYVE1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE_ZFYV1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger FYVE domain-containing protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000719">
        <property type="entry name" value="Zinc finger FYVE domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042427">
        <property type="entry name" value="ZFYV1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047856">
        <property type="entry name" value="ZFYVE1_first_BBox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047855">
        <property type="entry name" value="ZFYVE1_second_BBox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46624">
        <property type="entry name" value="AGAP002036-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46624:SF3">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
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    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="598"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="715"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004348"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="777" mass="86955" checksum="38D45F739AC70914" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMS1</accession>
    <name>A0A4W2DMS1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMS1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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    </dbReference>
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        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA PRIME-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35825:SF2">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA'-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="263"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
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        </location>
    </feature>
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        <location>
            <begin position="603"/>
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        </location>
    </feature>
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        <location>
            <begin position="678"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="725" mass="80542" checksum="CEF658CC0E624107" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT07</accession>
    <name>A0A4W2DT07_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Oxytocin receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">OXTR</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 13">Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027378858.1">
        <property type="nucleotide sequence ID" value="XM_027523057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT07"/>
    <dbReference type="SMR" id="A0A4W2DT07"/>
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                <person name="Tearle R."/>
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            <begin position="4378"/>
            <end position="4391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="5002" mass="554984" checksum="57546A2C2D5E6F2E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYQ2</accession>
    <name>A0A4W2DYQ2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Actin beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ACTB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383816.1">
        <property type="nucleotide sequence ID" value="XM_027528015.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2DYQ2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000031939.1"/>
        <property type="gene ID" value="ENSBIXG00000014387.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022501.1">
        <property type="protein sequence ID" value="ENSBIXP00005012881.1"/>
        <property type="gene ID" value="ENSBIXG00005017151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883879"/>
    <dbReference type="CTD" id="60"/>
    <dbReference type="GeneTree" id="ENSGT00950000182960"/>
    <dbReference type="OMA" id="PNIMVGM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044305">
        <property type="term" value="C:calyx of Held"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030863">
        <property type="term" value="C:cortical cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:glutamatergic synapse"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:lamellipodium"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:postsynaptic actin cytoskeleton"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:kinesin binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:establishment or maintenance of cell polarity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051621">
        <property type="term" value="P:regulation of norepinephrine uptake"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903076">
        <property type="term" value="P:regulation of protein localization to plasma membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900242">
        <property type="term" value="P:regulation of synaptic vesicle endocytosis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150111">
        <property type="term" value="P:regulation of transepithelial transport"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
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        <property type="entry name" value="Actin_CS"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0558">Oxidation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031939.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="375" mass="41737" checksum="6AFD05CA94E360E2" modified="2019-09-18" version="1">MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZU2</accession>
    <name>A0A4W2DZU2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZU2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043755.1">
        <property type="protein sequence ID" value="ENSBIXP00000032031.1"/>
        <property type="gene ID" value="ENSBIXG00000014491.1"/>
    </dbReference>
    <dbReference type="OMA" id="SYCTESH"/>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="68" mass="7311" checksum="FE2614D0E27FCDB7" modified="2019-09-18" version="1">SLMAEMISRGVGRARLTHLQTDVEHHGSHDVEVGEVDTQAPGQVEENEQRAGQPLAEDPIRAGGGRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E012</accession>
    <name>A0A4W2E012_BOBOX</name>
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        <submittedName>
            <fullName evidence="1">G protein subunit gamma 4</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="primary">GNG4</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E012"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005052.1">
        <property type="protein sequence ID" value="ENSBIXP00000030103.1"/>
        <property type="gene ID" value="ENSBIXG00000011926.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="124" mass="13037" checksum="9055BBA2A4CF54A1" modified="2019-09-18" version="1">MGGPDPCFPLIVLDSLCRWSFCKCRWWVDIGVSLTVNVAPSLLFTCSSQAGQGHVSSLQIPLHPASSYSGSCPAGAALIRACSGHSTGPCRISLGWPLPQRSESGGATHHGASTDQSIHMYPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4D7</accession>
    <name>A0A4W2E4D7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Chromodomain helicase DNA binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CHD3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000031600.1"/>
        <property type="gene ID" value="ENSBIXG00000013947.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CD1_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18662">
        <property type="entry name" value="CD2_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18055">
        <property type="entry name" value="DEXHc_CHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15531">
        <property type="entry name" value="PHD1_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15532">
        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000037">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000017">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000011">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 4 isoform X1"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012957">
        <property type="entry name" value="CHD_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009462">
        <property type="entry name" value="CHD_II_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012958">
        <property type="entry name" value="CHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF9">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08074">
        <property type="entry name" value="CHDCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06461">
        <property type="entry name" value="CHDII_SANT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08073">
        <property type="entry name" value="CHDNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06465">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01146">
        <property type="entry name" value="DUF1086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01147">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="437"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="514"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="19">
        <location>
            <begin position="594"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="19">
        <location>
            <begin position="689"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="806"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="1122"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="289"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="363"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="645"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="750"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
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        </source>
    </evidence>
    <sequence length="2059" mass="233036" checksum="F0ABBC486B318163" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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</entry>
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    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Beta subunit of voltage-dependent calcium channels which contributes to the function of the calcium channel by increasing peak calcium current. Plays a role in shifting voltage dependencies of activation and inactivation of the channel. May modulate G protein inhibition. May contribute to beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force. Involved in membrane targeting of the alpha-1 subunit CACNA1C.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1S) and the ancillary subunits CACNB1 or CACNB2, CACNG1 and CACNA2D1. The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio, i.e. it contains either CACNB1 or CACNB2. Interacts with CACNA1C. Interacts with RRAD; interaction may be involved in beta-adrenergic regulation of heart rate and contractile force. Interaction with RRAD regulates the trafficking of CACNA1C to the cell membrane. Interacts with TMIGD2. Interacts with CAMK2D. Interacts with CBARP. Interacts with CAMK2A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027415622.1">
        <property type="nucleotide sequence ID" value="XM_027559821.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000034867.1"/>
        <property type="gene ID" value="ENSBIXG00000006359.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005052327.1">
        <property type="protein sequence ID" value="ENSBIXP00005043270.1"/>
        <property type="gene ID" value="ENSBIXG00005005335.1"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182837"/>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="SH3_CACNB2"/>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CAB1-4_N_A-dom"/>
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    <dbReference type="InterPro" id="IPR035605">
        <property type="entry name" value="CACNB2_SH3"/>
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    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="VDCC_L_b2su"/>
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        <property type="entry name" value="VDCC_L_bsu"/>
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        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01628">
        <property type="entry name" value="LCACHANNELB2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="60"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="136"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="433"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="504"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="554"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="576"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="604" mass="67900" checksum="C8B03DFE704C5F87" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6U1</accession>
    <name>A0A4W2E6U1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable ATP-dependent RNA helicase DHX34</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DEAH box protein 34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">DExH-box helicase 34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DHX34</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a complex with RUVBL1 and RUVBL2. Part of a complex composed of SMG1, DHX34 and UPF1; within the complex DHX34 acts as a scaffolding protein to facilitate SMG1 phosphorylation of UPF1. Interacts with UPF1, MOV10, EIF4A3, XRN2, SMG6, SMG7, SMG9, UPF3A, UPF3B, CASC3/MLN51, XRN1, DIS3 and DCP1A; the interactions are RNA-independent. Interacts with NCBP1/CPB80; the interaction is RNA-dependent. Interacts (via C-terminus) with SMG1; the interaction is RNA-independent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
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        <property type="component" value="Chromosome 18"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="DEXHc_DHX34"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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    <dbReference type="InterPro" id="IPR056382">
        <property type="entry name" value="DHX34_Znf-C2H2"/>
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    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
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    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24485">
        <property type="entry name" value="zf-C2H2_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="172"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="368"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="733"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1091"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1094"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074012"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1146" mass="128760" checksum="A28D95CC6FD0E093" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8M1</accession>
    <name>A0A4W2E8M1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Apoptosis regulator Bcl-2 family BH4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E8M1"/>
    <dbReference type="STRING" id="30522.A0A4W2E8M1"/>
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        <property type="gene ID" value="ENSBIXG00000003367.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial outer membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of apoptotic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:release of cytochrome c from mitochondria"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003093">
        <property type="entry name" value="Bcl2_BH4"/>
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    <dbReference type="InterPro" id="IPR020731">
        <property type="entry name" value="Bcl2_BH4_motif_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF12">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02180">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01864">
        <property type="entry name" value="APOPREGBCLX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00265">
        <property type="entry name" value="BH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apoptosis regulator Bcl-2 family BH4" evidence="7">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="BH4" evidence="6">
        <location>
            <begin position="5"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="173" mass="19465" checksum="878768D2A0F41C90" modified="2019-09-18" version="1">MSQSNRELVVDFLSYKLSQKGYSWSQFSDMEENRTETPEGTESDMEIPSAINGNPSWHVADSPAVNGAIGHSRSLDARKMIPMTTVKQAVREASNEFELRYQQTFSDLTSQLHITPGTAYQSFEQENGDWDTFVELYENNTTESQKSQESFNCWSLMDVSVAGVVPLGLLFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EA77</accession>
    <name>A0A4W2EA77_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C2CD2 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">C2CD2L</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EA77"/>
    <dbReference type="SMR" id="A0A4W2EA77"/>
    <dbReference type="STRING" id="30522.A0A4W2EA77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009674.1">
        <property type="protein sequence ID" value="ENSBIXP00000028666.1"/>
        <property type="gene ID" value="ENSBIXG00000009944.1"/>
    </dbReference>
    <dbReference type="OMA" id="SWAFKDC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035774">
        <property type="term" value="P:positive regulation of insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21683">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040885">
        <property type="entry name" value="SMP_C2CD2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF8">
        <property type="entry name" value="PHOSPHOLIPID TRANSFER PROTEIN C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18696">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="268"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="707" mass="75980" checksum="62C05678ED27BDFE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECT5</accession>
    <name>A0A4W2ECT5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">FERM, ARHGEF and pleckstrin domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">FERM, RhoGEF and pleckstrin domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">FARP1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Interacts with CADM1. Interacts with RAC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
            <location evidence="15">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECT5"/>
    <dbReference type="STRING" id="30522.A0A4W2ECT5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028083.1">
        <property type="protein sequence ID" value="ENSBIXP00000036979.1"/>
        <property type="gene ID" value="ENSBIXG00000020488.1"/>
    </dbReference>
    <dbReference type="OMA" id="PHCLTLC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13193">
        <property type="entry name" value="FERM_C_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01220">
        <property type="entry name" value="PH1_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13235">
        <property type="entry name" value="PH2_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000040">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000005">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000021">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000046">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041788">
        <property type="entry name" value="FARP1/FARP2/FRMD7_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051835">
        <property type="entry name" value="RAC1-GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858">
        <property type="entry name" value="FERM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858:SF2">
        <property type="entry name" value="FERM, ARHGEF AND PLECKSTRIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="FERM" evidence="22">
        <location>
            <begin position="40"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="21">
        <location>
            <begin position="541"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="20">
        <location>
            <begin position="761"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="20">
        <location>
            <begin position="933"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="361"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="865"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="472"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="884"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1046" mass="118241" checksum="4A97EFB9F81BCA48" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFL0</accession>
    <name>A0A4W2EFL0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFL0"/>
    <dbReference type="STRING" id="30522.A0A4W2EFL0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023355.1">
        <property type="protein sequence ID" value="ENSBIXP00000034956.1"/>
        <property type="gene ID" value="ENSBIXG00000018067.1"/>
    </dbReference>
    <dbReference type="OMA" id="SKTMYQG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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        <property type="entry name" value="mRING-HC-C3HC3D_Roquin1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="roquin-1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="roquin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Roquin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ROQ_II"/>
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        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052249">
        <property type="entry name" value="Roquin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139">
        <property type="entry name" value="RING FINGER AND CCCH-TYPE ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139:SF6">
        <property type="entry name" value="ROQUIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18386">
        <property type="entry name" value="ROQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21206">
        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="494"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1093"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1095"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1118"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1132" mass="125567" checksum="13164AE178D4825A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGA2</accession>
    <name>A0A4W2EGA2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Neuronal calcium sensor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Frequenin homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NCS1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGA2"/>
    <dbReference type="STRING" id="30522.A0A4W2EGA2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046932.1">
        <property type="protein sequence ID" value="ENSBIXP00000036372.1"/>
        <property type="gene ID" value="ENSBIXG00000007245.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001780.1">
        <property type="protein sequence ID" value="ENSBIXP00005032420.1"/>
        <property type="gene ID" value="ENSBIXG00005012934.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154645"/>
    <dbReference type="OMA" id="KAQQRAC"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008048">
        <property type="term" value="F:calcium sensitive guanylate cyclase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF198">
        <property type="entry name" value="NEURONAL CALCIUM SENSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="240"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="324"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="370" mass="39708" checksum="D7ED33774BA95C5B" modified="2019-09-18" version="1">PRPPGPGTDEAGGGGAPLRGPGSALPGAADPPAQARPGGRVRGGRWRRALSGGSGSTPGAPVRAGGGERRGGGESKAQQRACAAELSNQGRPRRQRGRVGAQPSAAPRRGARRGRRPDGPGLPRGAPAPRPRRPGADKGAAPARPGPPPPQLRAPRPGPAAPAGCPDGVRPGARGPRPPRMGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGA8</accession>
    <name>A0A4W2EGA8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase-associated protein LEO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LEO1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEO1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027409800.1">
        <property type="nucleotide sequence ID" value="XM_027553999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGA8"/>
    <dbReference type="STRING" id="30522.A0A4W2EGA8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026598.1">
        <property type="protein sequence ID" value="ENSBIXP00000036382.1"/>
        <property type="gene ID" value="ENSBIXG00000019815.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046059.1">
        <property type="protein sequence ID" value="ENSBIXP00005035360.1"/>
        <property type="gene ID" value="ENSBIXG00005016513.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900325"/>
    <dbReference type="CTD" id="123169"/>
    <dbReference type="GeneTree" id="ENSGT00550000074952"/>
    <dbReference type="OMA" id="RSDRSMH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001711">
        <property type="term" value="P:endodermal cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045638">
        <property type="term" value="P:negative regulation of myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019827">
        <property type="term" value="P:stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="549"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="549"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="638"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="668" mass="75738" checksum="5B7FC20864F5CFCD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EI83</accession>
    <name>A0A4W2EI83_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EI83"/>
    <dbReference type="STRING" id="30522.A0A4W2EI83"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026969.1">
        <property type="protein sequence ID" value="ENSBIXP00000036507.1"/>
        <property type="gene ID" value="ENSBIXG00000019997.1"/>
    </dbReference>
    <dbReference type="OMA" id="HPPAQTM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20043">
        <property type="entry name" value="FH_FOXB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000082">
        <property type="entry name" value="forkhead box protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047389">
        <property type="entry name" value="FOXB1_B2_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF215">
        <property type="entry name" value="FORKHEAD BOX PROTEIN B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="322" mass="33794" checksum="E20CC7B49FC2A0ED" modified="2019-09-18" version="1">MPRPGKSSYSDQKPPYSYISLTAMAIQHSAEKMLPLSDIYKFIMERFPYYREHTQRWQNSLRHNLSFNDCFIKIPRRPDQPGKGSFWALHPDCGDMFENGSFLRRRKRFKVLRSDHPHLQAGSTKSAVGRLSQFPPYGLGSAAAAAAAAAASTSGFKHPFAIENIIGRDYKGVLQAGGLPLASVMHHLGYPVPGQLGNVVSSVWPHVGVMDSVAAAAAAAAAAGVPVGPEYGAFGVPVKALCHSASQSLPAVPVPIKPTPALPPVAALPPTLAVPGASQQPPAPSTVCPAAAASPAAPLLEPTAPGAAETKGGSLHSVLVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJH5</accession>
    <name>A0A4W2EJH5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:angiotensin-mediated vasodilation involved in regulation of systemic arterial blood pressure"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:phospholipase C-activating angiotensin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd15110">
        <property type="entry name" value="7tmA_MrgprH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="proto-oncogene Mas"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
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    <dbReference type="InterPro" id="IPR000820">
        <property type="entry name" value="Proto-oncogene_Mas"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11334:SF61">
        <property type="entry name" value="PROTO-ONCOGENE MAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00533">
        <property type="entry name" value="MASONCOGENE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="89"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="166"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="49"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="325" mass="37278" checksum="B6285E2AC1B315E9" modified="2019-09-18" version="1">MDEANGTSFVAEEPSNASTSGNTSVKDPRREIPIVHWVIMSISPLGFVENGILLWFLCFRMRRNPFTVYITHLSIADISLLFCIFILSVDYALDYELSSGYYYTIVTLSVTFLFGYNTGLYLLTAISVERCLSVLYPIWYRCHRPKHQSAFVCALLWALSCLVTTMEYVMCIDSEGQTHSRGDCRAVIIFIAILSFLVFTPLMVVSSTILVVKIRKNSWASHSSKLYLVITVTIIIFFIFAMPMRLLYLLYYEYWSTFKNLHHVSLLFSTINSSANPFIYFFVGSSRKKRFKESLKVVLTRAFKDEMQPRQPEDHTATTATETVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERZ7</accession>
    <name>A0A4W2ERZ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113891795</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ERZ7"/>
    <dbReference type="STRING" id="30522.A0A4W2ERZ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049384.1">
        <property type="protein sequence ID" value="ENSBIXP00000039742.1"/>
        <property type="gene ID" value="ENSBIXG00000023687.1"/>
    </dbReference>
    <dbReference type="OMA" id="QKSIPFY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="37"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="307" mass="33669" checksum="88DE56D1AD536DB5" modified="2019-09-18" version="1">MKTWVSEFILLGLSSDWGTQVSLFVLFLAMYLVTIVGNALILLLIRLDSRLHTPMYFFLSVLSLVDLCYSSSIVPQMLAHLLSVQKSIPFYSCLIQLSTSLALAASEFLLLGAMAYDRYVAVCYPLHYTVIMHGALCLGLAVGCLGAGLTNSLLETVITFRLPLCHSVMNHFACETLAVLRLACVDISFNKVMVAISGFLVIMLPFFLVLFSYGRIVAAILHIHSAQGRSKAFGTCASHLTVVSMCFGAAIFTYLGPRSAYSVDGEKMVALFYAVVAPMLNPLIYSLRNKEVMAALSKLLDKFRDKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ET61</accession>
    <name>A0A4W2ET61_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ankyrin repeat and SOCS box protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ASB10</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027394371.1">
        <property type="nucleotide sequence ID" value="XM_027538570.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027394372.1">
        <property type="nucleotide sequence ID" value="XM_027538571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ET61"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049687.1">
        <property type="protein sequence ID" value="ENSBIXP00000040106.1"/>
        <property type="gene ID" value="ENSBIXG00000024153.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890992"/>
    <dbReference type="CTD" id="136371"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000154">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000228">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000383">
        <property type="entry name" value="Ankyrin repeat and SOCS box protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050663">
        <property type="entry name" value="Ankyrin-SOCS_Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193:SF121">
        <property type="entry name" value="ADA2A-CONTAINING COMPLEX COMPONENT 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="13">
        <location>
            <begin position="405"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="452" mass="49174" checksum="0507C0D0693536B6" modified="2019-09-18" version="1">MPGSRSSPPQRGRLLGCPPSAPAGQFWGAWSHEAQERPGRPPLLCPDMALQNALYTGDLARLQELFPPHSTADLLLESRAAEPRWTSCQRGLWSLTYEEELTTPLHVAASRGHVEILRLLLRRRARPDSAPGGRTALHEACAAGHAACVHVLLVAGADPNIPDQDGRRPLHLCGGAGSLECADLLLRFGAKVDGRSEEEEETPLHVAARLGHVELADLLLRRGACPDARDVEGWTPLLAACDARCMSPADAEATTARCLQLCRLLLSAGADADAADKDRQRPLHLACRRGHADVVELLLARGVGANAMDYGGHTALHCALQGPAAALAQSPERTVRALLNHGAVRVWPGALPKVLERWCTSPRTIEVLMNTYSVVKLPEDAVGLVPPETLQKHHRFYSSLFALVRQPRSLQHLSRCALRAHLAACLPHALPRLPLPPRLLRYLQLDFEDVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EU52</accession>
    <name>A0A4W2EU52_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EU52"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049856.1">
        <property type="protein sequence ID" value="ENSBIXP00000040332.1"/>
        <property type="gene ID" value="ENSBIXG00000024372.1"/>
    </dbReference>
    <dbReference type="OMA" id="SIFLICP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="68" mass="7045" checksum="C6552E7C045CE395" modified="2019-09-18" version="1">PEALFCPYGLPLGSPRAGAALLTRPASFVPHGAGRGAPVVFALCAHPIPKRRGCFSHCGLFKPPAHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWJ3</accession>
    <name>A0A4W2EWJ3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tetratricopeptide repeat protein 21B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Intraflagellar transport 139 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TTC21B</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). Essential for retrograde trafficking of IFT-1, IFT-B and GPCRs. Negatively modulates the SHH signal transduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the IFT complex A (IFT-A) complex. IFT-A complex is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which is associated with TULP3 and a peripheral subcomplex composed of IFT43:WDR35:TTC21B. Interacts directy with WDR35 and TTC21B. Interacts with TTC25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TTC21 family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EWJ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038233.1">
        <property type="protein sequence ID" value="ENSBIXP00000041414.1"/>
        <property type="gene ID" value="ENSBIXG00000002109.1"/>
    </dbReference>
    <dbReference type="OMA" id="NATCVRA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000197">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000219">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000245">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000493">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056832">
        <property type="entry name" value="ARM_TT21_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056836">
        <property type="entry name" value="ARM_TT21_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056835">
        <property type="entry name" value="ARM_TT21_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056834">
        <property type="entry name" value="ARM_TT21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011717">
        <property type="entry name" value="TPR-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040364">
        <property type="entry name" value="TTC21A/TTC21B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699">
        <property type="entry name" value="STI2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25058">
        <property type="entry name" value="ARM_TT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25060">
        <property type="entry name" value="ARM_TT21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25068">
        <property type="entry name" value="ARM_TT21_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25064">
        <property type="entry name" value="ARM_TT21_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25063">
        <property type="entry name" value="ARM_TT21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07721">
        <property type="entry name" value="TPR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="15">
        <location>
            <begin position="10"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B second ARM" evidence="14">
        <location>
            <begin position="269"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="722"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fourth ARM" evidence="18">
        <location>
            <begin position="758"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fifth ARM repeats" evidence="17">
        <location>
            <begin position="952"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="952"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B C-terminal ARM" evidence="16">
        <location>
            <begin position="1102"/>
            <end position="1310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00084066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF25064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1315" mass="151052" checksum="66BC24B40A67A3FD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EX34</accession>
    <name>A0A4W2EX34_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EX34"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038742.1">
        <property type="protein sequence ID" value="ENSBIXP00000041632.1"/>
        <property type="gene ID" value="ENSBIXG00000001903.1"/>
    </dbReference>
    <dbReference type="OMA" id="QCSTNAV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="422" mass="49124" checksum="E97B8BF8C5EE5935" modified="2019-09-18" version="1">MTIISTTAGRNPSEEMEQPSWSTRVQNAVLGYNLKNDRMISVRFQGKPFNITVIQVYAPTSKAEEAEVERFYEDLQDLLELIPKKDVLFITGDWNAKLGSQDTPGVSDKFGLGIRNEAGQRLREFCQENALVIANTLFQQHKRRLYTWTSPEGQHRNQTDYILCSQRWRSSIQSTKTRPGADCGSDHELLITKFRLKLKKVGKTTRPFRYDLNQIPYDYIVEVRNRFKGLDLIDRVPDELWNEVRDIVQETGIKTIPMEKKCKKAKWLSGEALQIAVKRREAKSKGEKERYKHLNAEFQRIARRDKKAFLSNQCKEIEENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITDLEPDILECEVKWALESITTNKASGDDRIPVELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EX44</accession>
    <name>A0A4W2EX44_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">KIF2C</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EX44"/>
    <dbReference type="STRING" id="30522.A0A4W2EX44"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038783.1">
        <property type="protein sequence ID" value="ENSBIXP00000041654.1"/>
        <property type="gene ID" value="ENSBIXG00000025790.1"/>
    </dbReference>
    <dbReference type="OMA" id="RTTLECH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000006">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054473">
        <property type="entry name" value="KIF2A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF25">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22923">
        <property type="entry name" value="KIF2A-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="13 22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7 21" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="20 21" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="24">
        <location>
            <begin position="257"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="347"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>ATP</name>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046888"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="724" mass="81121" checksum="A4841CFE057E91ED" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2U1</accession>
    <name>A0A4W2F2U1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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            <topology evidence="2 14">Multi-pass membrane protein</topology>
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    </dbReference>
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        <property type="entry name" value="OLFACTORY RECEPTOR"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        </location>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="308" mass="34030" checksum="41D483EEBE8D7A1D" modified="2019-09-18" version="1">ITGNLTSVSEFFLLGLSDDPELEPLLCVLFLSMYLVTVLGNLLIILAITSDPRLHTPMYFFLSNLSLADISFISTTIPKMIVNIQSHSRVISYAGCLTQMSTLILFGYMDCTLLTVMAYDRFVAICHPLHYTAIMNPRLCCSLVLVSFCVSLLDSQVHNMIVLQLSCFKNVEISSFFCDPSQLLNLACSDTLKNSIVRYIIGAMSGFIPTSGIFFSYYKILVSILRVPSSGGRYKAFSTCGSHLSVVCLFYGTGIGVYISSALSSSPRKSAVASVMYTVVIPMLNPFIYSLRNKDIKSSLWRIGHRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3J0</accession>
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        <recommendedName>
            <fullName evidence="2">Cytoplasmic FMR1-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CYFIP1</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1 2">Belongs to the CYFIP family.</text>
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    <dbReference type="SMR" id="A0A4W2F3J0"/>
    <dbReference type="STRING" id="30522.A0A4W2F3J0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042474.1">
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        <property type="gene ID" value="ENSBIXG00005012955.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of lamellipodium assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051388">
        <property type="term" value="P:positive regulation of neurotrophin TRK receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:Rac protein signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of modification of postsynaptic actin cytoskeleton"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of translation at postsynapse, modulating synaptic transmission"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008153">
        <property type="entry name" value="FMR1_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01698">
        <property type="entry name" value="CYTOFMRPINTP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="3">
        <location>
            <begin position="64"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR008153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1253" mass="145191" checksum="CE14AA4F096670C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F8B7</accession>
    <name>A0A4W2F8B7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Calcium binding protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CABP7</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with PI4KB. This binding competes with FREQ/NCS1 binding in a calcium-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network membrane</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027422159.1">
        <property type="nucleotide sequence ID" value="XM_027566358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F8B7"/>
    <dbReference type="SMR" id="A0A4W2F8B7"/>
    <dbReference type="STRING" id="30522.A0A4W2F8B7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001728.1">
        <property type="protein sequence ID" value="ENSBIXP00000007490.1"/>
        <property type="gene ID" value="ENSBIXG00000013443.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014016.1">
        <property type="protein sequence ID" value="ENSBIXP00005001554.1"/>
        <property type="gene ID" value="ENSBIXG00005008047.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907332"/>
    <dbReference type="CTD" id="164633"/>
    <dbReference type="GeneTree" id="ENSGT00940000159368"/>
    <dbReference type="OMA" id="TQQIKQT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000115">
        <property type="entry name" value="Calcium-binding protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051111">
        <property type="entry name" value="Ca-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46311:SF1">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46311">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="215" mass="24453" checksum="9C254C955F7AFFA8" modified="2019-09-18" version="1">MPFHPVTAALMYRGIYTVPNLLSEQRPVDIPEDELEEIREAFKVFDRDGNGFISKQELGTAMRSLGYMPNEVELEVIIQRLDMDGDGQVDFEEFVTLLGPKLSTSGIPEKFHGTDFDTVFWKCDMQKLTVDELKRLLYDTFCEHLSMKDIENIIMTEEESHLGTAEECPVDVETCSNQQIRQTCVRKSLICAFAIAFIISVMLIAANQVLRSGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FIX0</accession>
    <name>A0A4W2FIX0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Discoidin, CUB and LCCL domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DCBLD1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FIX0"/>
    <dbReference type="STRING" id="30522.A0A4W2FIX0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019362.1">
        <property type="protein sequence ID" value="ENSBIXP00000033251.1"/>
        <property type="gene ID" value="ENSBIXG00000002433.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008183.1">
        <property type="protein sequence ID" value="ENSBIXP00005004514.1"/>
        <property type="gene ID" value="ENSBIXG00005000746.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157334"/>
    <dbReference type="OMA" id="HDGDYQR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF1">
        <property type="entry name" value="DISCOIDIN, CUB AND LCCL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="10">
        <location>
            <begin position="135"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="12">
        <location>
            <begin position="246"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="11">
        <location>
            <begin position="349"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="715"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="804" mass="87223" checksum="03C628938D7FBD19" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FZA8</accession>
    <name>A0A4W2FZA8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">5'-3' exonuclease PLD3</fullName>
            <ecNumber evidence="26">3.1.16.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="36">(S,S)-bis(monoacylglycero)phosphate synthase PLD3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Phospholipase D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="39" type="primary">PLD3</name>
    </gene>
    <organism evidence="39 41">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="39 40 41" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>3-lyso-sn-glycero-1-phospho-(3'-(9Z-octadecenoyl)-1'-sn-glycerol) + 1-(9Z-octadecenoyl)-sn-glycerol = 3-(9Z-octadecenoyl)-sn-glycero-1-phospho-(3'-(9Z-octadecenoyl)-1'-sn-glycerol) + glycerol</text>
            <dbReference type="Rhea" id="RHEA:82567"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:75757"/>
            <dbReference type="ChEBI" id="CHEBI:139150"/>
            <dbReference type="ChEBI" id="CHEBI:232394"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:82568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates.</text>
            <dbReference type="EC" id="3.1.16.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>an (R,S)-glycero-3-phospho-(3'-acyl-1'-glycerol) + a 1-acyl-sn-glycerol = a 3-acyl-sn-glycero-1-phospho-(3'-acyl-1'-sn-glycerol) + glycerol</text>
            <dbReference type="Rhea" id="RHEA:82563"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:64683"/>
            <dbReference type="ChEBI" id="CHEBI:77717"/>
            <dbReference type="ChEBI" id="CHEBI:232393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:82564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>a 5'-end 5'-dephospho-2'-deoxyribonucleotidyl-2'-deoxyribonucleotide in single-stranded DNA + H2O = a 5'-end dephospho-2'-deoxyribonucleoside in single-stranded DNA + a 2'-deoxyribonucleoside 3'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81379"/>
            <dbReference type="Rhea" id="RHEA-COMP:19701"/>
            <dbReference type="Rhea" id="RHEA-COMP:19702"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:131705"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:231873"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:81380"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>a 5'-end 5'-dephospho-ribonucleotidyl-ribonucleotide-RNA + H2O = a ribonucleoside 3'-phosphate + a 5'-end dephospho-ribonucleoside-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81375"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:19670"/>
            <dbReference type="ChEBI" id="CHEBI:13197"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:231871"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:81376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 5'-end 5'-phospho-2'-deoxyribonucleotide in single-stranded DNA + H2O = a 5'-end 5'-dephospho-2'-deoxyribonucleotide in single-stranded DNA + phosphate</text>
            <dbReference type="Rhea" id="RHEA:82335"/>
            <dbReference type="Rhea" id="RHEA-COMP:19868"/>
            <dbReference type="Rhea" id="RHEA-COMP:19869"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:82336"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>a ribonucleoside 3'-phosphate-2'-3'-cyclophospho-GMP + H2O = a ribonucleoside 3'-phosphate + 2',3'-cyclophospho-GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81319"/>
            <dbReference type="ChEBI" id="CHEBI:13197"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:60837"/>
            <dbReference type="ChEBI" id="CHEBI:231870"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:81320"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="35">Homodimer. Interacts with APP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="25">Early endosome membrane</location>
            <topology evidence="25">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Late endosome membrane</location>
            <topology evidence="24">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.16.1" evidence="26"/>
    <dbReference type="RefSeq" id="XP_027371605.1">
        <property type="nucleotide sequence ID" value="XM_027515804.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371606.1">
        <property type="nucleotide sequence ID" value="XM_027515805.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371607.1">
        <property type="nucleotide sequence ID" value="XM_027515806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FZA8"/>
    <dbReference type="SMR" id="A0A4W2FZA8"/>
    <dbReference type="STRING" id="30522.A0A4W2FZA8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020732.1">
        <property type="protein sequence ID" value="ENSBIXP00000033829.1"/>
        <property type="gene ID" value="ENSBIXG00000016647.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045346.1">
        <property type="protein sequence ID" value="ENSBIXP00005010790.1"/>
        <property type="gene ID" value="ENSBIXG00005015402.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876502"/>
    <dbReference type="CTD" id="23646"/>
    <dbReference type="GeneTree" id="ENSGT00950000183059"/>
    <dbReference type="OMA" id="RDNHTHF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09144">
        <property type="entry name" value="PLDc_vPLD3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000013">
        <property type="entry name" value="phospholipase D3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000019">
        <property type="entry name" value="phospholipase D3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032803">
        <property type="entry name" value="PLDc_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF16">
        <property type="entry name" value="5'-3' EXONUCLEASE PLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13918">
        <property type="entry name" value="PLDc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="20" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17 37" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="22" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 37" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 37" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="38">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="38">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00035759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00037797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00037858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00039059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00039647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00041681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00049873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00049874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00049884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00050052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="490" mass="54619" checksum="8D5F78A548AD3BE8" modified="2019-09-18" version="1">MKPKLMYQELKVPAEEPASELPMNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLDFPNASTSNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEASAQQGEEVLRQLQTLAPRGVKVRIAVSKPNGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRPYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYIAVMNYLPIMEFSHPRRFWPAIDDGLRRAAYERGVKVRLLISCWGHSDPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADDAQARIPYARVNHNKYMVTERATYIGTSNWSGSYFTETAGTSLLVTQNGRGGLRSQLEAVFLRDWDSPYSHDLDAAADSVGNACRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G2N5</accession>
    <name>A0A4W2G2N5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Growth hormone secretagogue receptor type 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GH-releasing peptide receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ghrelin receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GHSR</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Also binds other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396760.1">
        <property type="nucleotide sequence ID" value="XM_027540959.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027396771.1">
        <property type="nucleotide sequence ID" value="XM_027540970.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027396782.1">
        <property type="nucleotide sequence ID" value="XM_027540981.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027396792.1">
        <property type="nucleotide sequence ID" value="XM_027540991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G2N5"/>
    <dbReference type="SMR" id="A0A4W2G2N5"/>
    <dbReference type="STRING" id="30522.A0A4W2G2N5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049959.1">
        <property type="protein sequence ID" value="ENSBIXP00000040438.1"/>
        <property type="gene ID" value="ENSBIXG00000024506.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021346.1">
        <property type="protein sequence ID" value="ENSBIXP00005012125.1"/>
        <property type="gene ID" value="ENSBIXG00005016403.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892285"/>
    <dbReference type="CTD" id="2693"/>
    <dbReference type="GeneTree" id="ENSGT01120000271823"/>
    <dbReference type="OMA" id="IGNLMTM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001616">
        <property type="term" value="F:growth hormone secretagogue receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016520">
        <property type="term" value="F:growth hormone-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046697">
        <property type="term" value="P:decidualization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002031">
        <property type="term" value="P:G protein-coupled receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030252">
        <property type="term" value="P:growth hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048009">
        <property type="term" value="P:insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032691">
        <property type="term" value="P:negative regulation of interleukin-1 beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032715">
        <property type="term" value="P:negative regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032720">
        <property type="term" value="P:negative regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032100">
        <property type="term" value="P:positive regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043568">
        <property type="term" value="P:positive regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040018">
        <property type="term" value="P:positive regulation of multicellular organism growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051963">
        <property type="term" value="P:regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032094">
        <property type="term" value="P:response to food"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15131">
        <property type="entry name" value="7tmA_GHSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000125">
        <property type="entry name" value="growth hormone secretagogue receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003905">
        <property type="entry name" value="GHS-R/MTLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF7">
        <property type="entry name" value="GROWTH HORMONE SECRETAGOGUE RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01417">
        <property type="entry name" value="GHSRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="60"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="366" mass="41215" checksum="26C3A9CB002346DC" modified="2019-09-18" version="1">MWNATRSEEPGPNLTLPDLGWDALPDNDSLTDELPPLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWHYRPWNLGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVITKGRVKLVILVIWAVAFCSAGPIFVLVGVEHENGTDPRDTNECRATEFAVRSGLLTVMVWVSSVFFFLPVFCLTVLYSLIGRKLWRRRRGEAAVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSVEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFKLLGFEPFSQRKLSTLKDESSRAWTESSINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G3H7</accession>
    <name>A0A4W2G3H7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase II subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">POLR2C</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="7">Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.</text>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="71" mass="7668" checksum="4699679DEBB67CA9" modified="2019-09-18" version="1">MKSSGTLMLLISVAYLFILAEAVSQRGSQAFCSNYEKTATNGKPCPKTDKPVCGTDGQTYHNLCEFCKAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GFH2</accession>
    <name>A0A4W2GFH2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GFH2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011411.1">
        <property type="protein sequence ID" value="ENSBIXP00000002677.1"/>
        <property type="gene ID" value="ENSBIXG00000009138.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029381.1">
        <property type="protein sequence ID" value="ENSBIXP00005017493.1"/>
        <property type="gene ID" value="ENSBIXG00005005676.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000187477"/>
    <dbReference type="OMA" id="QRTETIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="122" mass="13750" checksum="0825FD2FF86F6A4F" modified="2019-09-18" version="1">VSAFFLCISHITEISHVTIFHFAVALLKAFELGPALFRSSEEATLSQLLSHGPIQLALPMTGLQCFVKPSLMEYVKESLGFLVAPVHTCHSRIKFKDTRCLQPFKGKVQRYKLSVAFIHYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GH83</accession>
    <name>A0A4W2GH83_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21 25">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="7 25">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CBS</name>
    </gene>
    <organism evidence="27 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 25">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 25">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="7 25"/>
    <dbReference type="RefSeq" id="XP_027402853.1">
        <property type="nucleotide sequence ID" value="XM_027547052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GH83"/>
    <dbReference type="SMR" id="A0A4W2GH83"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052100.1">
        <property type="protein sequence ID" value="ENSBIXP00000025275.1"/>
        <property type="gene ID" value="ENSBIXG00000005751.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048714.1">
        <property type="protein sequence ID" value="ENSBIXP00005017388.1"/>
        <property type="gene ID" value="ENSBIXG00005020947.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895630"/>
    <dbReference type="CTD" id="875"/>
    <dbReference type="GeneTree" id="ENSGT00510000047027"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04608">
        <property type="entry name" value="CBS_pair_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000014">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046353">
        <property type="entry name" value="CBS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 25" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 24 25" id="KW-0129">CBS domain</keyword>
    <keyword evidence="18 25" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="19 25" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 25" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CBS" evidence="26">
        <location>
            <begin position="420"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="571" mass="62839" checksum="B2BF93C4C2A02F83" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GQV1</accession>
    <name>A0A4W2GQV1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZDHHC22</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027408315.1">
        <property type="nucleotide sequence ID" value="XM_027552514.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027408316.1">
        <property type="nucleotide sequence ID" value="XM_027552515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GQV1"/>
    <dbReference type="STRING" id="30522.A0A4W2GQV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049008.1">
        <property type="protein sequence ID" value="ENSBIXP00000019560.1"/>
        <property type="gene ID" value="ENSBIXG00000004556.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034630.1">
        <property type="protein sequence ID" value="ENSBIXP00005021012.1"/>
        <property type="gene ID" value="ENSBIXG00005024019.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899156"/>
    <dbReference type="CTD" id="283576"/>
    <dbReference type="GeneTree" id="ENSGT00730000111268"/>
    <dbReference type="OMA" id="GQTWCQL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="112"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="263" mass="29331" checksum="9911B53F5734F4BE" modified="2019-09-18" version="1">MLALRLLNVVAPAYFLCIALVTLVLQLFLFLPRMREDPTAARLFSPALLHGALFLFLSANALGNYVLVIQNSPDDLDACQGAEARRALCPPPSTHFCRVCARVTLRHDHHCFFTGNCIGSRNMRNFILFCLYTSLACLYSMVAGVAYISAVLSISFAHPLAFLTLLPTSISQFFSGAVLGSEMFVILMLYLWFAIGLACAGFCCHQLLLILRGQTRHQVRKGVAVRARPWRKNLQEVFGKRWLLGLLVPMFNVGSESCKQRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GSZ4</accession>
    <name>A0A4W2GSZ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aldehyde dehydrogenase 1A1</fullName>
            <ecNumber evidence="12">1.2.1.19</ecNumber>
            <ecNumber evidence="10">1.2.1.28</ecNumber>
            <ecNumber evidence="11">1.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">3-deoxyglucosone dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">ALDH-E1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">ALHDII</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="39">Aldehyde dehydrogenase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Aldehyde dehydrogenase family 1 member A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="38">Aldehyde dehydrogenase family 1 member A3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Aldehyde dehydrogenase, cytosolic</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Retinal dehydrogenase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="37">Retinaldehyde dehydrogenase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="44" type="primary">ALDH1A3</name>
    </gene>
    <organism evidence="44 46">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="44 45 46" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="36">Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. High specificity for all-trans-retinal as substrate, can also accept acetaldehyde as substrate in vitro but with lower affinity. Required for the biosynthesis of normal levels of retinoate in the embryonic ocular and nasal regions; a critical lipid in the embryonic development of the eye and the nasal region.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(E)-4-hydroxynon-2-enal + NAD(+) + H2O = (E)-4-hydroxynon-2-enoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58968"/>
            <dbReference type="ChEBI" id="CHEBI:142920"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:67249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>11-cis-retinal + NAD(+) + H2O = 11-cis-retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:47132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16066"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:87435"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:47133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>13-cis-retinal + NAD(+) + H2O = 13-cis-retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67332"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45487"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:169952"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:67333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3-deoxyglucosone + NAD(+) + H2O = 2-dehydro-3-deoxy-D-gluconate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67244"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="ChEBI" id="CHEBI:60777"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:67245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>4-aminobutanal + NAD(+) + H2O = 4-aminobutanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58264"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="1.2.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:19106"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>9-cis-retinal + NAD(+) + H2O = 9-cis-retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78273"/>
            <dbReference type="ChEBI" id="CHEBI:78630"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:42085"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>acetaldehyde + NAD(+) + H2O = acetate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25294"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:25295"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>all-trans-13,14-dihydroretinal + NAD(+) + H2O = all-trans-13,14-dihydroretinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:194182"/>
            <dbReference type="ChEBI" id="CHEBI:194183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:75120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>all-trans-retinal + NAD(+) + H2O = all-trans-retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:35291"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:42081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:16186"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>benzaldehyde + NAD(+) + H2O = benzoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16150"/>
            <dbReference type="ChEBI" id="CHEBI:17169"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:11841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>hexanal + NAD(+) + H2O = hexanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17120"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:88528"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:67277"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>malonaldehyde + NAD(+) + H2O = 3-oxopropanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:566274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:67253"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>propanal + NAD(+) + H2O = propanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67256"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17153"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:67257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>retinal + NAD(+) + H2O = retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16177"/>
            <dbReference type="ChEBI" id="CHEBI:15035"/>
            <dbReference type="ChEBI" id="CHEBI:15036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:16178"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; retinol metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with PRMT3; the interaction is direct, inhibits ALDH1A1 aldehyde dehydrogenase activity and is independent of the methyltransferase activity of PRMT3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 41">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
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    <dbReference type="EC" id="1.2.1.28" evidence="10"/>
    <dbReference type="EC" id="1.2.1.36" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027377206.1">
        <property type="nucleotide sequence ID" value="XM_027521405.1"/>
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    <dbReference type="SMR" id="A0A4W2GSZ4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000009151.1"/>
        <property type="gene ID" value="ENSBIXG00000014869.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035209.1">
        <property type="protein sequence ID" value="ENSBIXP00005021386.1"/>
        <property type="gene ID" value="ENSBIXG00005024423.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879702"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158815"/>
    <dbReference type="OMA" id="GTYAINW"/>
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        <property type="component" value="Chromosome 21"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019145">
        <property type="term" value="F:aminobutyraldehyde dehydrogenase (NAD+) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018479">
        <property type="term" value="F:benzaldehyde dehydrogenase (NAD+) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:NAD+ binding"/>
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        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="term" value="F:retinal dehydrogenase (NAD+) activity"/>
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        <property type="term" value="F:thyroid hormone binding"/>
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        <property type="term" value="P:apoptotic process"/>
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        <property type="term" value="P:face development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:Harderian gland development"/>
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        <property type="term" value="P:inner ear morphogenesis"/>
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    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0021768">
        <property type="term" value="P:nucleus accumbens development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060166">
        <property type="term" value="P:olfactory pit development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002072">
        <property type="term" value="P:optic cup morphogenesis involved in camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002138">
        <property type="term" value="P:retinoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060013">
        <property type="term" value="P:righting reflex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07141">
        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000054">
        <property type="entry name" value="Aldehyde dehydrogenase family 1 member A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 41" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="43">
        <location>
            <begin position="40"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="42">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="40">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00051881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00052527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00055664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00071373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00075938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00079201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="512" mass="56184" checksum="03ED8510858EF4D2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GYG7</accession>
    <name>A0A4W2GYG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trafficking protein particle complex subunit 6B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRAPPC6B</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of a transport protein particle (TRAPP) complex that may function in specific stages of inter-organelle traffic. Specifically involved in the early development of neural circuitry, likely by controlling the frequency and amplitude of intracellular calcium transients implicated in the regulation of neuron differentiation and survival.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Part of a TRAPP complex. Heterodimer with TRAPPC3. The heterodimer TRAPPC6B-TRAPPC3 interacts with TRAPPC1 likely providing a core for TRAPP complex formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377738.1">
        <property type="nucleotide sequence ID" value="XM_027521937.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000009557.1"/>
        <property type="gene ID" value="ENSBIXG00000015216.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037142.1">
        <property type="protein sequence ID" value="ENSBIXP00005022689.1"/>
        <property type="gene ID" value="ENSBIXG00005004347.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879999"/>
    <dbReference type="CTD" id="122553"/>
    <dbReference type="GeneTree" id="ENSGT00390000012948"/>
    <dbReference type="OMA" id="CKEFWTA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000004">
        <property type="entry name" value="Trafficking protein particle complex subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="158" mass="17983" checksum="5A8B0474548C304B" modified="2019-09-18" version="1">MADEALFLLLHNEMVSGVYKSAEQGEVENGRCITKLENMGFRVGQGLIERFTKDTARFKDELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQMSAGKQYLEHASKYLAFTCGLIRGGLSNLGIKSIVTAEVSSMPACKFQVMIQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H079</accession>
    <name>A0A4W2H079_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-binding protein inhibitor ID-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inhibitor of DNA binding 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Inhibitor of differentiation 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ID1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation. Interacts with CLOCK and BMAL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027414682.1">
        <property type="nucleotide sequence ID" value="XM_027558881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H079"/>
    <dbReference type="SMR" id="A0A4W2H079"/>
    <dbReference type="STRING" id="30522.A0A4W2H079"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029152.1">
        <property type="protein sequence ID" value="ENSBIXP00000037441.1"/>
        <property type="gene ID" value="ENSBIXG00000021011.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051784.1">
        <property type="protein sequence ID" value="ENSBIXP00005023241.1"/>
        <property type="gene ID" value="ENSBIXG00005026026.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903153"/>
    <dbReference type="CTD" id="3397"/>
    <dbReference type="GeneTree" id="ENSGT00940000161109"/>
    <dbReference type="OMA" id="LDMKGCY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032963">
        <property type="term" value="P:collagen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072577">
        <property type="term" value="P:endothelial cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001886">
        <property type="term" value="P:endothelial cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060425">
        <property type="term" value="P:lung morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060426">
        <property type="term" value="P:lung vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120163">
        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000352">
        <property type="term" value="P:negative regulation of endothelial cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045668">
        <property type="term" value="P:negative regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045765">
        <property type="term" value="P:regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19691">
        <property type="entry name" value="bHLH_dnHLH_ID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000039">
        <property type="entry name" value="DNA-binding protein inhibitor ID-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026052">
        <property type="entry name" value="DNA-bd_prot-inh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11723">
        <property type="entry name" value="DNA-BINDING PROTEIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11723:SF4">
        <property type="entry name" value="DNA-BINDING PROTEIN INHIBITOR ID-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="52"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="154" mass="15929" checksum="5130B71C7C9FDFAB" modified="2019-09-18" version="1">MKVASGSAAAAAGPSCALKAGKAAGGAGEVVRCLSEQSVAISRCAGGPGARLPALLEEQPVNVLLYDMNGCYSRLKELVPTLPQNRKVSRVEILQHVIDYIWDLELELNSESHVGTPGGRGLPARAPLSTLNGEIGALAAEAACVPADDRILCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H7Y7</accession>
    <name>A0A4W2H7Y7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Apoptosis regulator Bcl-2 family BH4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H7Y7"/>
    <dbReference type="STRING" id="30522.A0A4W2H7Y7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032561.1">
        <property type="protein sequence ID" value="ENSBIXP00000038928.1"/>
        <property type="gene ID" value="ENSBIXG00000022789.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014886.1">
        <property type="protein sequence ID" value="ENSBIXP00005028114.1"/>
        <property type="gene ID" value="ENSBIXG00005029502.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278332"/>
    <dbReference type="OMA" id="HLCHKLN"/>
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        <property type="entry name" value="APOPREGBCLX"/>
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        <property type="entry name" value="BCL2FAMILY"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apoptosis regulator Bcl-2 family BH4" evidence="6">
        <location>
            <begin position="10"/>
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            <begin position="10"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50063"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="195" mass="22095" checksum="5CA6557854CA3A5D" modified="2019-09-18" version="1">IFIMKMSQSNRRLVVDFLSYKLSQKGYSWSRFNDVEENRTEASEGTKSDMETPMPSMTTHLGTWRIAFSDQTSQLHITPGTAYQSFEQMMYELLQDGVNWGRIVAFFSFGGALCMESIDKETQVLVSQSTTWMATYLNDHLESWIQENGGWDTSVKLCENNTTTESQKGQERFNSWSLTGMTSAGTALLGLLLNS</sequence>
</entry>
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    <accession>A0A4W2HEW5</accession>
    <name>A0A4W2HEW5_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Centrosomal protein 85</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">CEP85</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014216.1">
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        <property type="gene ID" value="ENSBIXG00005008011.1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CENTROSOMAL PROTEIN OF 85 KDA"/>
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    </feature>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        <location>
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        <location>
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        <location>
            <begin position="1"/>
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    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF24555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="764" mass="85724" checksum="51209421863E2A6A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYL6</accession>
    <name>A0A4W2HYL6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="gene ID" value="ENSBIXG00000018765.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045896.1">
        <property type="protein sequence ID" value="ENSBIXP00005035135.1"/>
        <property type="gene ID" value="ENSBIXG00005016230.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="54"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="241" mass="26461" checksum="C5AA180E5BDB6C55" modified="2019-09-18" version="1">MGLNQLTSFRKQTRSERKSSGSRLSHCFRYRQIGQKRRQSEDIVVEISVLFAVHVPMNKGLSGGKSFACFSPHKLYVDLLSACLLINLYAGQEATVRTGHGTTDWFKIGKGVCQGCVLSPCLFNLYAEYIMRNAGLEETQAGIKIAGRSVSNLGYADNTTLMAESEEELKSLLMKVESGKVGLKLNIQKTKIMASGPITSWQIDGETVETVSDFIFLGSKSLQMVTAAMKLKDACSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I3F0</accession>
    <name>A0A4W2I3F0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcriptional adapter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TADA2A</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a distant site. Binds double-stranded DNA. Binds dinucleosomes, probably at the linker region between neighboring nucleosomes. Plays a role in chromatin remodeling. May promote TP53/p53 'Lys-321' acetylation, leading to reduced TP53 stability and transcriptional activity. May also promote XRCC6 acetylation thus facilitating cell apoptosis in response to DNA damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027372717.1">
        <property type="nucleotide sequence ID" value="XM_027516916.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027372718.1">
        <property type="nucleotide sequence ID" value="XM_027516917.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027372719.1">
        <property type="nucleotide sequence ID" value="XM_027516918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I3F0"/>
    <dbReference type="SMR" id="A0A4W2I3F0"/>
    <dbReference type="STRING" id="30522.A0A4W2I3F0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011664.1">
        <property type="protein sequence ID" value="ENSBIXP00000002566.1"/>
        <property type="gene ID" value="ENSBIXG00000009033.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031820.1">
        <property type="protein sequence ID" value="ENSBIXP00005039912.1"/>
        <property type="gene ID" value="ENSBIXG00005022404.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877115"/>
    <dbReference type="CTD" id="6871"/>
    <dbReference type="GeneTree" id="ENSGT00940000156751"/>
    <dbReference type="OMA" id="YNGNHRP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000110">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000020">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="15">
        <location>
            <begin position="12"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="17">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="18">
        <location>
            <begin position="74"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="14">
        <location>
            <begin position="74"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="16">
        <location>
            <begin position="356"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="347"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="443" mass="51469" checksum="07C5F1AB43636FA0" modified="2019-09-18" version="1">MDRLGSFSSDPSDKPPCRGCSSYLMEPYIKCAECGPPPFFLCLQCFTRGFEYKKHQSDHTYEIMTSDFPVLDPSWTAQEEMALLEAVMDCGFGNWQDVANQMCTKTKEECEKHYMKHFINNPLFASTLLNLKQAEEAKTADTAIPFHSADDPPRPTFDSLLSRDMAGYMPARADFIEEFDNYAEWDLRDIDFVEDDSDILHALKMAVVDIYHSRLKERQRRKKIIRDHGLINLRKFQLMERRYPKEVQDLYETMRRFARIVGPVEHDKFIESHALEFELRREIKRLQEYRTAGITNFCSARTYDHLKKTREEERLKRTMLSEVLQYIQDSSACQQWLRRQADIDSGLSPSVPMTSNSGRRSAPPLNLTGLPGTEKLNEKEKELCQMVRLVPGAYLEYKSALLNECNKQGGLRLAQARALIKIDVNKTRKIYDFLIREGYITKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I464</accession>
    <name>A0A4W2I464_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I464"/>
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        <property type="protein sequence ID" value="ENSBIXP00000036701.1"/>
        <property type="gene ID" value="ENSBIXG00000020188.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028843.1">
        <property type="protein sequence ID" value="ENSBIXP00005038526.1"/>
        <property type="gene ID" value="ENSBIXG00005020565.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271669"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="21" mass="2244" checksum="C47DC3868919DE11" modified="2019-09-18" version="1">SSTWIKVFGEGTKLVVIPPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I655</accession>
    <name>A0A4W2I655_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Fructose-2,6-bisphosphatase TIGAR</fullName>
            <ecNumber evidence="5">3.1.3.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">TP53-induced glycolysis and apoptosis regulator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">TP53-induced glycolysis regulatory phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TIGAR</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <dbReference type="EC" id="3.1.3.46"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with HK2; the interaction increases hexokinase HK2 activity in a hypoxia- and HIF1A-dependent manner, resulting in the regulation of mitochondrial membrane potential, thus increasing NADPH production and decreasing intracellular ROS levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027396862.1">
        <property type="nucleotide sequence ID" value="XM_027541061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I655"/>
    <dbReference type="SMR" id="A0A4W2I655"/>
    <dbReference type="STRING" id="30522.A0A4W2I655"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001164.1">
        <property type="protein sequence ID" value="ENSBIXP00000031413.1"/>
        <property type="gene ID" value="ENSBIXG00000008028.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030716.1">
        <property type="protein sequence ID" value="ENSBIXP00005039387.1"/>
        <property type="gene ID" value="ENSBIXG00005021786.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892323"/>
    <dbReference type="CTD" id="57103"/>
    <dbReference type="GeneTree" id="ENSGT00390000013224"/>
    <dbReference type="OMA" id="PTIQCVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010659">
        <property type="term" value="P:cardiac muscle cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071279">
        <property type="term" value="P:cellular response to cobalt ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019661">
        <property type="term" value="P:glucose catabolic process to lactate via pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060576">
        <property type="term" value="P:intestinal epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904024">
        <property type="term" value="P:negative regulation of glucose catabolic process to lactate via pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901525">
        <property type="term" value="P:negative regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000378">
        <property type="term" value="P:negative regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010666">
        <property type="term" value="P:positive regulation of cardiac muscle cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905857">
        <property type="term" value="P:positive regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902153">
        <property type="term" value="P:regulation of response to DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010332">
        <property type="term" value="P:response to gamma radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002931">
        <property type="term" value="P:response to ischemia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000026">
        <property type="entry name" value="Putative fructose-2,6-bisphosphatase TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="17">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="17">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="270" mass="29467" checksum="2D738CBE454EA6D8" modified="2019-09-18" version="1">MTRFALTVVRHGETRLNKEKIIQGQGIDEPLSETGFKQAAAAGIFLKDVKFTHVFSSDLTRTKQTVHGILEKSKFCKDMTVKYDSRLRERKYGVAEGRPLSELRAMAKAAGEECPAFTPPGGETLDQLKRRGKDFFEFLCQLILKEAGQNEQFSQEAPSSCLESSLAEIFPLGKNCASTFNSDSGTPGLAASVLVVSHGAYIRSLLDYFLTDLKCSFPATLSRSELTSVSPNTGMTVFILNFEKGGKGRPTAQCVCVNLQGHLAGVNKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I7S8</accession>
    <name>A0A4W2I7S8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Teashirt zinc finger homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TSHZ2</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the teashirt C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I7S8"/>
    <dbReference type="STRING" id="30522.A0A4W2I7S8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026395.1">
        <property type="protein sequence ID" value="ENSBIXP00000036245.1"/>
        <property type="gene ID" value="ENSBIXG00000003526.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029551.1">
        <property type="protein sequence ID" value="ENSBIXP00005038872.1"/>
        <property type="gene ID" value="ENSBIXG00005021102.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183051"/>
    <dbReference type="OMA" id="TGHYQDN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027008">
        <property type="entry name" value="Teashirt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058631">
        <property type="entry name" value="TSHZ1-3_homeodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487:SF3">
        <property type="entry name" value="TEASHIRT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487">
        <property type="entry name" value="TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26094">
        <property type="entry name" value="HTH_TSHZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="360"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="1011"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="102"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="324"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="517"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="701"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="821"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1047"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="544"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="714"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1057"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1070"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1080" mass="119279" checksum="F1A8C74B5E391A1D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ILU0</accession>
    <name>A0A4W2ILU0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/SPRY domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">F-box only protein 45</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FBXO45</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a complex with MYCBP2 and SKP1. Interacts with HEY1; leading to FBXO45 nuclear translocation. Interacts (via SPRY domain) with CDH2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FBXO45/Fsn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027393131.1">
        <property type="nucleotide sequence ID" value="XM_027537330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ILU0"/>
    <dbReference type="SMR" id="A0A4W2ILU0"/>
    <dbReference type="STRING" id="30522.A0A4W2ILU0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048884.1">
        <property type="protein sequence ID" value="ENSBIXP00000039015.1"/>
        <property type="gene ID" value="ENSBIXG00000022937.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043182.1">
        <property type="protein sequence ID" value="ENSBIXP00005045170.1"/>
        <property type="gene ID" value="ENSBIXG00005028962.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889916"/>
    <dbReference type="CTD" id="200933"/>
    <dbReference type="GeneTree" id="ENSGT01030000234629"/>
    <dbReference type="OMA" id="ATKRASM"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099524">
        <property type="term" value="C:postsynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099523">
        <property type="term" value="C:presynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021960">
        <property type="term" value="P:anterior commissure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021799">
        <property type="term" value="P:cerebral cortex radially oriented cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021800">
        <property type="term" value="P:cerebral cortex tangential migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021957">
        <property type="term" value="P:corticospinal tract morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000300">
        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060386">
        <property type="term" value="P:synapse assembly involved in innervation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22111">
        <property type="entry name" value="F-box_FBXO45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12907">
        <property type="entry name" value="SPRY_Fbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000024">
        <property type="entry name" value="F-box/SPRY domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000017">
        <property type="entry name" value="F-box/SPRY domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035784">
        <property type="entry name" value="SPRY_FBXO45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF7">
        <property type="entry name" value="F-BOX_SPRY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="7">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="87"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="281" mass="30305" checksum="7EBDADA2B432942C" modified="2019-09-18" version="1">MAAPAPGAGAASGGAGCSGGGAGAGSGSGAGGRLPSRVLELVFSYLELSELRSCALVCKHWYRCLHGDENSEVWRSLCARSLAEEALRTDILCNLPSYKAKIRAFQHAFSTNDCSRNVYIKKNGFTLHRNPIAQSTDGARTKIGFSEGRHAWEVWWEGPLGTVAVIGIATKRAPMQCQGYVALLGSDDQSWGWNLVDNNLLHNGEVNGSFPQCNNAPKYQIGERIRVILDMEDKTLAFERGYEFLGVAFRGLPKVCLYPAVSAVYGNTEVTLVYLGKPLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMB4</accession>
    <name>A0A4W2BMB4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 130</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TMEM130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMB4"/>
    <dbReference type="STRING" id="30522.A0A4W2BMB4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053733.1">
        <property type="protein sequence ID" value="ENSBIXP00000000139.1"/>
        <property type="gene ID" value="ENSBIXG00000030205.1"/>
    </dbReference>
    <dbReference type="OMA" id="PPLTMCW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000668">
        <property type="entry name" value="transmembrane protein 130 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045219">
        <property type="entry name" value="PKAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046846">
        <property type="entry name" value="PKAT_KLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11861">
        <property type="entry name" value="MELANOCYTE PROTEIN PMEL 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11861:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20433">
        <property type="entry name" value="PKAT_KLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021428088" description="Transmembrane protein 130" evidence="10">
        <location>
            <begin position="25"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="471" mass="51842" checksum="6D0CEAC890CEB319" modified="2019-09-18" version="1">MAPALWPRLGRILWLACLLPLAPARVAAGLYELQLTTDGPATTGAEVTIMASLVADDNGSLALPTSTHLYRFHWIHTPLLLTGKADEAFSSTIRVVGSVPGDFPISVWVTAADCGMCQPVARSLLVLPITGSSSTLKYFPRIDLKACTFPSGPGHLWRQKVQGPGERGTGANCTCKIGKFLVGNLVVTQNTSLPWPSSYLTKTVLKVSFLLHDPSNFFKNASFLYSWDFGDGTQMVTRDGTVYYNYSIIGSFTVKVKVVAGWEQVTPDAGKGILQKTGDFSASLKLQETLRGIQVFGPNLIQTFQKMVVTLNFLGSPPLNVCWRLKPECLPLEEGECHPVSVDSTAYNLTHIFRDPGDYCFSIQAENVISKTHQYHKIQVWPSSIQPAVFAFPCATLITMMLAFIMYMTLRNAAHQKDMVENPEPPTGVRCCCQMCCGPFLLETQSEYLEVVRENHGLLPPLYKSVKTYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMW1</accession>
    <name>A0A4W2BMW1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heme-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N-methylprotoporphyrin with similar affinities.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMW1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053990.1">
        <property type="protein sequence ID" value="ENSBIXP00000000018.1"/>
        <property type="gene ID" value="ENSBIXG00000030366.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010068.1">
        <property type="protein sequence ID" value="ENSBIXP00005003563.1"/>
        <property type="gene ID" value="ENSBIXG00005009694.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160320"/>
    <dbReference type="OMA" id="NDKXQFG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000003">
        <property type="entry name" value="Heme-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF22">
        <property type="entry name" value="HEME-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="191" mass="21142" checksum="6B49DC1830BDD351" modified="2019-09-18" version="1">MLGMIKNSLFGSVETWPWQVLSKGSKGDVFYEERACEGGKFATVEVTDKPVDEALREAMPKVMKYVGGSNDKGLGMGMTVPISFAVFPSDDGDVQKKLKVWSRIPNKFQSDPPAPSDDSIKIEDREGITVYSTQFGGYAKAADYTAQAAQLRSALESTAKYQTDCYFCTGYDPPMKPYGRRNEVWLVKSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNB2</accession>
    <name>A0A4W2BNB2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone-lysine N-methyltransferase SETMAR</fullName>
            <ecNumber evidence="12">2.1.1.357</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113880535</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60308"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.357"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.357" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNB2"/>
    <dbReference type="SMR" id="A0A4W2BNB2"/>
    <dbReference type="STRING" id="30522.A0A4W2BNB2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013974.1">
        <property type="protein sequence ID" value="ENSBIXP00000001463.1"/>
        <property type="gene ID" value="ENSBIXG00000028533.1"/>
    </dbReference>
    <dbReference type="OMA" id="VDSMVPK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140954">
        <property type="term" value="F:histone H3K36 dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10544">
        <property type="entry name" value="SET_SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000041">
        <property type="entry name" value="Histone-lysine N-methyltransferase SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="15">
        <location>
            <begin position="60"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="14">
        <location>
            <begin position="126"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="16">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="306" mass="34254" checksum="E48E2DB78D91261C" modified="2019-09-18" version="1">MATCEEVPEALKGQLDVARGLENLPVSAWPPGAEPEPFQYTPDHVAGPGADADPSQITFPGCACLKTPCLPGTCSCLRHENNYDDRSCLRDIGSEAKCTEPVFECNVLCQCSERCRNRVVQWGLQFHLQVFKTDHKGWGLRTLDFIPKGRFVCEYAGEVLGISEVQRRVQLQTIHDSNYIIAIREHVYNGQVMETFVDPASIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAARDILPEEELSYDYSGRFLNLMHSEDKERLDNGKLRKPCYCGARSCAAFLPYDSSLYCPTEKPDTSEEGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQI1</accession>
    <name>A0A4W2BQI1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein ABHD15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha/beta hydrolase domain-containing protein 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABHD15</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May regulate adipocyte lipolysis and liver lipid accumulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PDE3B; this interaction regulates PDE3B's stability and expression and, thereby, impacts the antilipolytic action of insulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027374292.1">
        <property type="nucleotide sequence ID" value="XM_027518491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQI1"/>
    <dbReference type="STRING" id="30522.A0A4W2BQI1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014786.1">
        <property type="protein sequence ID" value="ENSBIXP00000001093.1"/>
        <property type="gene ID" value="ENSBIXG00000028961.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019460.1">
        <property type="protein sequence ID" value="ENSBIXP00005034470.1"/>
        <property type="gene ID" value="ENSBIXG00005002264.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877876"/>
    <dbReference type="CTD" id="116236"/>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="OMA" id="AWSHEAT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060612">
        <property type="term" value="P:adipose tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000103">
        <property type="entry name" value="Abhydrolase domain-containing 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF39">
        <property type="entry name" value="PROTEIN ABHD15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610267" description="Protein ABHD15" evidence="11">
        <location>
            <begin position="25"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="468" mass="51836" checksum="896EFCF18EF8374A" modified="2019-09-18" version="1">MPPWGAALALLLAALALLCLLVLRLRRPGRRAVGARTLPGARGEDHGEEVDGGGPAGQFSDGREPLPGGCSLICKPSALAQCLLRALRRSAALEPGPRPWLSGPHLQTLCHFVLPVGPGSELAREYLQLADDGLVALDWVVGPGARGRRATNAGGLPAVLLVIPNAWGRLTRNVLGLCLLALERGYYPVIFHRRGHHGCPLVSPRLQPFGDPSDLKEAITYIRFRHPAAPLFAVSEGSGSALLLSYLGECGSSSYMTGAACISPVLRCREWFEAGLPWPYERGFLLHQKIALSRYATALEDTVDTGKLFRSRSLREFEETLFCHTKSFPISWDTYWDHNDPLRDVDEAAVPVLCICSADDPVCGPPNHFLPTELFHSNPYFFLLLSRHGGHCGFLRQEPLPAWSHEVTLEYFRALTDFFRTEERMKGLSRRRASFLAGRRRWGTLPKREVSPSSSLEEIFSWKRSYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BR27</accession>
    <name>A0A4W2BR27_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Putative olfactory receptor 10D4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113886046</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BR27"/>
    <dbReference type="STRING" id="30522.A0A4W2BR27"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011820.1">
        <property type="protein sequence ID" value="ENSBIXP00000002485.1"/>
        <property type="gene ID" value="ENSBIXG00000008951.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035776.1">
        <property type="protein sequence ID" value="ENSBIXP00005021759.1"/>
        <property type="gene ID" value="ENSBIXG00005024716.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244869"/>
    <dbReference type="OMA" id="MGNSHTI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="312" mass="34746" checksum="D0BD3014EDF347EA" modified="2019-09-18" version="1">VKMRNHTPVTEFLLTGIPHTQGLEHPLFVFFLAFYLLTLVGNLLILLATLTSSNLHTPMYFFLGNLSVFDIFFPSVSSPKMMLYLLGRSQTISYQGCACQLFFYHFLGCTECFLYTMMAYDRFAAICHPLRYTAIMNPRVCAILTLSTWMGSSVHASVLTFLVFKLPYCGPKEVGNFFCDIPVVLSLACADTSLAHRVSVTNVGVVALLCFLLVLASYTRIVISILRISSSEGRHRAFSTCSAHLTSVLLFYGPVILIYLRPASSPWLDSVVPLFNNIVNPSLNPLIYSLRNKDMKLALRKALIQGVHTCGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BR40</accession>
    <name>A0A4W2BR40_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FAD-dependent oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">FOXRED1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). Involved in mid-late stages of complex I assembly.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Associates with components of the mitochondrial respiratory chain complex I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027387587.1">
        <property type="nucleotide sequence ID" value="XM_027531786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BR40"/>
    <dbReference type="STRING" id="30522.A0A4W2BR40"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014256.1">
        <property type="protein sequence ID" value="ENSBIXP00000001333.1"/>
        <property type="gene ID" value="ENSBIXG00000028672.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040495.1">
        <property type="protein sequence ID" value="ENSBIXP00005024979.1"/>
        <property type="gene ID" value="ENSBIXG00005027555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885936"/>
    <dbReference type="CTD" id="55572"/>
    <dbReference type="GeneTree" id="ENSGT00390000006114"/>
    <dbReference type="OMA" id="PDHNALI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000155">
        <property type="entry name" value="FAD-dependent oxidoreductase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="17">
        <location>
            <begin position="65"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="486" mass="54086" checksum="0B368B72EEA7BB27" modified="2019-09-18" version="1">MLRRALRLRLGPCLSGRGLGTYRRGSTLDWDGKVSEIKKKIQSIFPGGAWNPLYDTSHLPPERSDVVIVGGGVLGLSVAYWLKRLEKQQGAIRVLVVERDHTYARASTVLSVGGIRQQFSLPQNVQLSLFSAEFLRNINEYLAVVDDPPLDLQFNPSGYLLLASEEGAAIMERNVKMQRQEGAKVCLMSPEQLQKKFPWINTEGVALASYGLENEGWFDPWCLLQGLRRKLQSMGVLFCQGEVTRFISSSSHMETASGEQLTLKRIHEVHVKMDHSQEFQPVECAIVVNAAGAWSGQIAELAGVGNGPPGTMQGTKLPVEPRKRYVYLWHCPQGPGLEAPLVADSSGAYFRREGLGNNYLGSCSPTEEEEPDPGNLEVDYDFFQEKVWPRLAQRVPAFETLKVRSAWAGYYDYNTFDQNGVVGPHPLVVNMYFATGFSGHGLQQAPAVGRAVAEMVLEGHFQTINLSPFLFSRFYFGEKAQEHCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUD2</accession>
    <name>A0A4W2BUD2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regulator of G-protein signaling 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha. May regulate extra and intracellular mitogenic signals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUD2"/>
    <dbReference type="STRING" id="30522.A0A4W2BUD2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009229.1">
        <property type="protein sequence ID" value="ENSBIXP00000003761.1"/>
        <property type="gene ID" value="ENSBIXG00000010152.1"/>
    </dbReference>
    <dbReference type="OMA" id="PFHAFLK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.196.10:FF:000001">
        <property type="entry name" value="Regulator of G-protein signaling 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF187">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="RGS" evidence="10">
        <location>
            <begin position="65"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="116" mass="13277" checksum="21A2FE35EEC16DF5" modified="2019-09-18" version="1">MCRTLAAFPTSCLERAKEFKTRLGIFLHKSELGSDTGSVGKFEWGSKHSKEGKNFSEDVLGWKESFDLLLSSKNGVAPFHAFLKTEFSEENLEFWLACEEFKKLRSKKKKKNPTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BW89</accession>
    <name>A0A4W2BW89_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN</fullName>
            <ecNumber evidence="20">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">WRN</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Interacts with PML (isoform PML-4). Interacts (via KBM motif) with XRCC5 and XRCC6; promoting recruitment to DNA damage sites. Interacts with RECQL5; this interaction stimulates WRN helicase activity on DNA fork duplexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="20"/>
    <dbReference type="STRING" id="30522.A0A4W2BW89"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007643.1">
        <property type="protein sequence ID" value="ENSBIXP00000004528.1"/>
        <property type="gene ID" value="ENSBIXG00000010859.1"/>
    </dbReference>
    <dbReference type="OMA" id="TYLIHMV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032392">
        <property type="term" value="P:DNA geometric change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18017">
        <property type="entry name" value="DEXHc_RecQ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06129">
        <property type="entry name" value="RNaseD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000369">
        <property type="entry name" value="Werner syndrome ATP-dependent helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000005">
        <property type="entry name" value="Werner syndrome ATP-dependent helicase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000053">
        <property type="entry name" value="Werner syndrome ATP-dependent helicase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000941">
        <property type="entry name" value="Werner syndrome RecQ like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001023">
        <property type="entry name" value="Werner syndrome RecQ like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029491">
        <property type="entry name" value="Helicase_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF120">
        <property type="entry name" value="BIFUNCTIONAL 3'-5' EXONUCLEASE_ATP-DEPENDENT HELICASE WRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14493">
        <property type="entry name" value="HTH_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="25">
        <location>
            <begin position="529"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="26">
        <location>
            <begin position="720"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="HRDC" evidence="24">
        <location>
            <begin position="1121"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1332"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1360"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="1398"/>
            <end position="1409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00064885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1409" mass="159802" checksum="FCBACF7264EE0D2C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYG6</accession>
    <name>A0A4W2BYG6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC113882304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GST superfamily. Alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYG6"/>
    <dbReference type="SMR" id="A0A4W2BYG6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005857.1">
        <property type="protein sequence ID" value="ENSBIXP00000005389.1"/>
        <property type="gene ID" value="ENSBIXG00000011595.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03208">
        <property type="entry name" value="GST_C_Alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03077">
        <property type="entry name" value="GST_N_Alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000005">
        <property type="entry name" value="Glutathione S-transferase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003080">
        <property type="entry name" value="GST_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF107">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01266">
        <property type="entry name" value="GSTRNSFRASEA"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="85"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="222" mass="25425" checksum="95EEF2749CB04650" modified="2019-09-18" version="1">MAGKPTLHYFNGRGRMECIRWLLAAAGVEFEEKFIEKPEDLDKLKNDGSLMFQQVPMVEIDGMKLVQTRAILNYIATKYNLYGKDMKERALIDMYSEGVADLGEMIMHFPLCPPAEKDAKLTLIREKTTNRYLPAFENVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDPSLLANFPLLKALKARVSSLPAVKKFLQPGSQRKPPTDEKKIEEARKVFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZD5</accession>
    <name>A0A4W2BZD5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Butyrophilin subfamily 1 member A1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZD5"/>
    <dbReference type="STRING" id="30522.A0A4W2BZD5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005070.1">
        <property type="protein sequence ID" value="ENSBIXP00000005786.1"/>
        <property type="gene ID" value="ENSBIXG00000006529.1"/>
    </dbReference>
    <dbReference type="OMA" id="RVERGYC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000208">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000040">
        <property type="entry name" value="Ret finger protein-like 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF64">
        <property type="entry name" value="BUTYROPHILIN, SUBFAMILY 3, MEMBER A3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="88"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="12">
        <location>
            <begin position="260"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="54283" checksum="15AE3E46F5E3E203" modified="2019-09-18" version="1">MELRWFRSKSLETVFVYQNRQEQKEEQMAPYAGRTSLVGDLLNQGYAAVHIQRVQASDNGLYTCFFRKGHFYEEAGLELKVAGVGSAPQVRITGPEEDGVRVVCTASGWFPKPQVQWRDLSGEKFLAFSEAHTQDAEGLFSVEAALVVRDSSVENVTCSILNPVLGREKAMAIFIPEPFFPQASPWKVAFSVSLTVLVILLLGAGCYTKREHSMKMQVRGEKETLCQTSEQDRQTKEEVLKDAAKFQEELERRKSAYLAAWRKAQLYADWRKEEFQEWPVTLDPGSAHSDLVVSHEKTSVTLKASCVNTADTCSVLGFEGITSGRCYWEVEIRDGDQSGWALGVCGEGVNRKGWYTESPDKGFWAVGRVERGYCACAVPQTPLSLMQAPHPRLRVGVFLDHQEGDVSFYNMTDGSHIFSFPQASFSGTLFPYFMIRYGDMSMTICSKVGGSEGLPVALSNPSLEEPVSLPGAGLSSGSGADGLPGAESPLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3B8</accession>
    <name>A0A4W2C3B8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113882075</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3B8"/>
    <dbReference type="STRING" id="30522.A0A4W2C3B8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002169.1">
        <property type="protein sequence ID" value="ENSBIXP00000007274.1"/>
        <property type="gene ID" value="ENSBIXG00000013241.1"/>
    </dbReference>
    <dbReference type="OMA" id="MWQDNSM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="35285" checksum="583B80602AD965DA" modified="2019-09-18" version="1">MNLNCSLWQDNSMSVKHFAFAKFSEVTEQCFLLFTLILLMFLASLTGNVLIALAIWTNPALHTPMYFFLANLSLLEIGYTCSVIPKMLQSLVSEARGISREGCATQMFFFTLFAISECCLLAAMAFDRYMAICSPLHYATRMSRGVCVHLAMISWGVGCIVGLGQTNYIFSLDFCGPCEIDHFFCDLPPILALACGDTSHNEAAVFVVATLCISSPFLLIIASYGRILAAVLIMPSPEGRQKALSTCSSHLLVVTLFYGSGSVTYLRPKASHSPGVDKLLALFYTAVTSMLNPIIYSLRNKEVKTALRRTLGKKKVCPIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3N3</accession>
    <name>A0A4W2C3N3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">inositol-polyphosphate 5-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.56</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">INPP5A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type I family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.56" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3N3"/>
    <dbReference type="STRING" id="30522.A0A4W2C3N3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003903.1">
        <property type="protein sequence ID" value="ENSBIXP00000006368.1"/>
        <property type="gene ID" value="ENSBIXG00000012481.1"/>
    </dbReference>
    <dbReference type="OMA" id="FGMETCT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09092">
        <property type="entry name" value="INPP5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039737">
        <property type="entry name" value="INPP5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12997:SF2">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-5-PHOSPHATASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12997">
        <property type="entry name" value="TYPE I INOSITOL-1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="412" mass="47699" checksum="B33BAAE1DFE9BDF1" modified="2019-09-18" version="1">MAGKAASPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHVHRPHFLALHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYKKVTGKEIYSDTLESTPMLEKEKFPQDYFPECKWSRKGFLRTRWCLADCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKALGYVLDRISDQRFQKASYFVFGDFNFRLDSKSVVETLCTKATMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDDNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDCSQGRQYMNTRCPAWCDRVLMSPSAKELVLRSESEEKVVTYDHIGPSVCMGDHKPVFLAFRIAPGAGKPHARVHKCCVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7Y4</accession>
    <name>A0A4W2C7Y4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7Y4"/>
    <dbReference type="STRING" id="30522.A0A4W2C7Y4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000766.1">
        <property type="protein sequence ID" value="ENSBIXP00000007943.1"/>
        <property type="gene ID" value="ENSBIXG00000001499.1"/>
    </dbReference>
    <dbReference type="OMA" id="MHRCELD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="199" mass="21574" checksum="03CA7F302FD63942" modified="2025-10-08" version="2">MKTRGRRTPRSTRPSCGCPEGGWRTNGHPCRLPPPRCRLPSAQHPSRRLQGSLDGRPSRIQAWPPEAQGPCAGRTRAGRIRDPAPPGGFLPQKTSLHGPACPPELPIPEGGQSCSSKSSGTGSSRPQRSLPLPPRIVGGLRAAQRAFGWGCGRLKDARRPLGPPRFLRPSPPSILKVRIVKAMIFPVVMHRCELDHKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAD1</accession>
    <name>A0A4W2CAD1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Mitochondrial sodium/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Na(+)/K(+)/Ca(2+)-exchange protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Sodium/calcium exchanger protein, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Sodium/potassium/calcium exchanger 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Solute carrier family 24 member 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Solute carrier family 8 member B1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3 Li(+)(out) + Ca(2+)(in) = 3 Li(+)(in) + Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:72631"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAD1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043805.1">
        <property type="protein sequence ID" value="ENSBIXP00000008830.1"/>
        <property type="gene ID" value="ENSBIXG00000014575.1"/>
    </dbReference>
    <dbReference type="OMA" id="IWIMNIA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099093">
        <property type="term" value="P:calcium export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000023">
        <property type="entry name" value="Mitochondrial sodium/calcium exchanger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000025">
        <property type="entry name" value="sodium/potassium/calcium exchanger 6, mitochondrial isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0452">Lithium</keyword>
    <keyword evidence="16 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 28" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021382707" description="Mitochondrial sodium/calcium exchanger protein" evidence="28">
        <location>
            <begin position="27"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="29">
        <location>
            <begin position="104"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="29">
        <location>
            <begin position="422"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00076738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00077141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00082808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="584" mass="64066" checksum="26524EC15B77DF91" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CD85</accession>
    <name>A0A4W2CD85_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dual specificity protein kinase TTK</fullName>
            <ecNumber evidence="1">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments. Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint. Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore. Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments. Phosphorylates KNL1, KNTC1 and autophosphorylates. Phosphorylates MCRS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and promotes chromosome alignment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with TPR; the interactions occurs in a microtubule-independent manner. Interacts with MAD1L1 and MAD2L1.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CD85"/>
    <dbReference type="STRING" id="30522.A0A4W2CD85"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044225.1">
        <property type="protein sequence ID" value="ENSBIXP00000009614.1"/>
        <property type="gene ID" value="ENSBIXG00000015266.1"/>
    </dbReference>
    <dbReference type="OMA" id="PISTPKW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14131">
        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000308">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000101">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027084">
        <property type="entry name" value="Mps1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="592"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="903"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="903"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="924" mass="103740" checksum="E46D5CF2E25CFEB8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CDQ5</accession>
    <name>A0A4W2CDQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein-sorting-associated protein 25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ESCRT-II complex subunit VPS25</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CDQ5"/>
    <dbReference type="STRING" id="30522.A0A4W2CDQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020166.1">
        <property type="protein sequence ID" value="ENSBIXP00000011031.1"/>
        <property type="gene ID" value="ENSBIXG00000002529.1"/>
    </dbReference>
    <dbReference type="OMA" id="TRCLIMW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000141">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.570:FF:000001">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="174" mass="20544" checksum="264153217FA28A28" modified="2019-09-18" version="1">MSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLTFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQVVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSKSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGB3</accession>
    <name>A0A4W2CGB3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RUN domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role as p53/TP53 inhibitor and thus may have oncogenic activity.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGB3"/>
    <dbReference type="STRING" id="30522.A0A4W2CGB3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020042.1">
        <property type="protein sequence ID" value="ENSBIXP00000010952.1"/>
        <property type="gene ID" value="ENSBIXG00000002514.1"/>
    </dbReference>
    <dbReference type="OMA" id="THKGNHW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="CDD" id="cd17683">
        <property type="entry name" value="RUN_RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000012">
        <property type="entry name" value="RUN domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058732">
        <property type="entry name" value="RUNDC1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194:SF5">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194">
        <property type="entry name" value="SORTING NEXIN-29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26030">
        <property type="entry name" value="RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
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    </dbReference>
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        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="6">
        <location>
            <begin position="421"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00055063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="613" mass="67508" checksum="133EB72D9F1CB7DF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIA4</accession>
    <name>A0A4W2CIA4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 18">Dihydroxyacetone phosphate acyltransferase</fullName>
            <shortName evidence="18">DAP-AT</shortName>
            <shortName evidence="18">DHAP-AT</shortName>
            <ecNumber evidence="15 18">2.3.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">GNPAT</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Dihydroxyacetonephosphate acyltransferase catalyzing the first step in the biosynthesis of plasmalogens, a subset of phospholipids that differ from other glycerolipids by having an alkyl chain attached through a vinyl ether linkage at the sn-1 position of the glycerol backbone, and which unique physical properties have an impact on various aspects of cell signaling and membrane biology.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dihydroxyacetone phosphate + an acyl-CoA = a 1-acylglycerone 3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:17657"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57534"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="2.3.1.42"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17658"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dihydroxyacetone phosphate + hexadecanoyl-CoA = 1-hexadecanoylglycerone 3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:40715"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:58303"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:40716"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10 18">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Part of a heterotrimeric complex composed of GNPAT, AGPS and a modified form of GNPAT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13 18">Peroxisome membrane</location>
            <topology evidence="13 18">Peripheral membrane protein</topology>
            <orientation evidence="13 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.42" evidence="15 18"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIA4"/>
    <dbReference type="STRING" id="30522.A0A4W2CIA4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000006043.1"/>
        <property type="gene ID" value="ENSBIXG00000012183.1"/>
    </dbReference>
    <dbReference type="OMA" id="RFNLEWY"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028353">
        <property type="entry name" value="DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500063">
        <property type="entry name" value="DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000437">
        <property type="entry name" value="GPAT_DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 18" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 18" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="19">
        <location>
            <begin position="156"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="680" mass="77663" checksum="93602604CB92854F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIU7</accession>
    <name>A0A4W2CIU7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIU7"/>
    <dbReference type="STRING" id="30522.A0A4W2CIU7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023249.1">
        <property type="protein sequence ID" value="ENSBIXP00000013029.1"/>
        <property type="gene ID" value="ENSBIXG00000003087.1"/>
    </dbReference>
    <dbReference type="OMA" id="FVMPACV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="98"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="371" mass="40820" checksum="AFAD88DB888A19D9" modified="2019-09-18" version="1">MRHGISPPEAAKFADVQSFTYQTLSLNLFLENGLNVSSGIPLWGAGVSAFTGKPLSWMPGRNSTVVTEFLLTVFADHPHWGLPLFAVFLGFYLLTLLGNCGMILLIRQDRRLHTPMYFFLGHLALVDICYSSTVVPQVLGVLLERGVVLSRARCATQFFLFTFFASMDCYLLAIMAYDRCVAVCRPLLYVAIVTEKARWVLVVGAYAAGFSSALIRTVTAFTLSFCGNNQIDFIFCDLPPLLKLSCGDSYTQEVVIIVFAVLVMPACILIILVSYAFIVLAIVQMRSSGGRAKTFSTCASHLAAVALFFGTLIFMYLRDNSGQSSEADRVVSVLYTVVSPMLNPLIYSLRNKEVKEAVLKSLSRPKVSGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMS9</accession>
    <name>A0A4W2CMS9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Gamma-crystallin F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC113881050</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379699.1">
        <property type="nucleotide sequence ID" value="XM_027523898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMS9"/>
    <dbReference type="SMR" id="A0A4W2CMS9"/>
    <dbReference type="STRING" id="30522.A0A4W2CMS9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023614.1">
        <property type="protein sequence ID" value="ENSBIXP00000013277.1"/>
        <property type="gene ID" value="ENSBIXG00000018237.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042582.1">
        <property type="protein sequence ID" value="ENSBIXP00005026369.1"/>
        <property type="gene ID" value="ENSBIXG00005028747.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881050"/>
    <dbReference type="GeneTree" id="ENSGT00940000158720"/>
    <dbReference type="OMA" id="YRRRQDW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF119">
        <property type="entry name" value="GAMMA-CRYSTALLIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="41"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="88"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="174" mass="21086" checksum="1ED266259EB6DC70" modified="2019-09-18" version="1">MGKITFYEDRGFQGRHYECSSDHSNLQPYFSRCNSIRVDSGCWMLYEQPNFQGPQYFLRRGDYPDYQQWMGLNDSIRSCRLIPHTGSHRLRIYEREDYRGQMVEITEDCSSLHDRFHFSEIHSFNVLEGWWVLYEMTNYRGRQYLLRPGDYRRYHDWGATNARVGSLRRAVDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPB2</accession>
    <name>A0A4W2CPB2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nascent polypeptide associated complex subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NACA</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027398068.1">
        <property type="nucleotide sequence ID" value="XM_027542267.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398069.1">
        <property type="nucleotide sequence ID" value="XM_027542268.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398070.1">
        <property type="nucleotide sequence ID" value="XM_027542269.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398071.1">
        <property type="nucleotide sequence ID" value="XM_027542270.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398072.1">
        <property type="nucleotide sequence ID" value="XM_027542271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CPB2"/>
    <dbReference type="SMR" id="A0A4W2CPB2"/>
    <dbReference type="STRING" id="30522.A0A4W2CPB2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026186.1">
        <property type="protein sequence ID" value="ENSBIXP00000014992.1"/>
        <property type="gene ID" value="ENSBIXG00000019585.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016760.1">
        <property type="protein sequence ID" value="ENSBIXP00005008987.1"/>
        <property type="gene ID" value="ENSBIXG00005014024.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892846"/>
    <dbReference type="CTD" id="4666"/>
    <dbReference type="GeneTree" id="ENSGT00440000033468"/>
    <dbReference type="OMA" id="SQKMIFA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14415">
        <property type="entry name" value="UBA_NACA_NACP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000002">
        <property type="entry name" value="Nascent polypeptide-associated complex (NAC), alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000006">
        <property type="entry name" value="Putative nascent polypeptide-associated complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="70"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="215" mass="23370" checksum="A507EC80FA348A0D" modified="2019-09-18" version="1">MPGEATDTVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ17</accession>
    <name>A0A4W2CQ17_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 22">DNA damage-inducible transcript 3 protein</fullName>
            <shortName evidence="22">DDIT-3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 22">C/EBP zeta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 22">C/EBP-homologous protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21 22">C/EBP-homologous protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 22">CCAAT/enhancer-binding protein homologous protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Growth arrest and DNA-damage-inducible protein GADD153</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">DDIT3</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="22">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Nucleus</location>
        </subcellularLocation>
        <text evidence="22">Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation.</text>
    </comment>
    <comment type="PTM">
        <text evidence="22">Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="22">Ubiquitinated, leading to its degradation by the proteasome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 22">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398153.1">
        <property type="nucleotide sequence ID" value="XM_027542352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQ17"/>
    <dbReference type="SMR" id="A0A4W2CQ17"/>
    <dbReference type="STRING" id="30522.A0A4W2CQ17"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026601.1">
        <property type="protein sequence ID" value="ENSBIXP00000015265.1"/>
        <property type="gene ID" value="ENSBIXG00000019818.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003924.1">
        <property type="protein sequence ID" value="ENSBIXP00005006670.1"/>
        <property type="gene ID" value="ENSBIXG00005012115.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892879"/>
    <dbReference type="CTD" id="1649"/>
    <dbReference type="GeneTree" id="ENSGT00390000006305"/>
    <dbReference type="OMA" id="MVNLHKA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990622">
        <property type="term" value="C:CHOP-ATF3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990617">
        <property type="term" value="C:CHOP-ATF4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036488">
        <property type="term" value="C:CHOP-C/EBP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008140">
        <property type="term" value="F:cAMP response element binding protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043522">
        <property type="term" value="F:leucine zipper domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140537">
        <property type="term" value="F:transcription regulator activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060840">
        <property type="term" value="P:artery development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001955">
        <property type="term" value="P:blood vessel maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070509">
        <property type="term" value="P:calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002086">
        <property type="term" value="P:diaphragm contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006983">
        <property type="term" value="P:ER overload response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160144">
        <property type="term" value="P:GDF15-GFRAL signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140468">
        <property type="term" value="P:HRI-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990442">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120163">
        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000016">
        <property type="term" value="P:negative regulation of determination of dorsal identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045599">
        <property type="term" value="P:negative regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032700">
        <property type="term" value="P:negative regulation of interleukin-17 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032713">
        <property type="term" value="P:negative regulation of interleukin-4 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045662">
        <property type="term" value="P:negative regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032689">
        <property type="term" value="P:negative regulation of type II interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902237">
        <property type="term" value="P:positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032757">
        <property type="term" value="P:positive regulation of interleukin-8 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061771">
        <property type="term" value="P:response to caloric restriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036119">
        <property type="term" value="P:response to platelet-derived growth factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904738">
        <property type="term" value="P:vascular associated smooth muscle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990874">
        <property type="term" value="P:vascular associated smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000066">
        <property type="entry name" value="DNA damage-inducible transcript 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016670">
        <property type="entry name" value="DNA_damage_induc_transcript_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16833">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 3 DDIT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16833:SF0">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016571">
        <property type="entry name" value="C/EBPzeta_CHOP_DDIT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0010">Activator</keyword>
    <keyword evidence="7 22" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17 22" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 22" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 22" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 22" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="16 22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 22" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 22" id="KW-0678">Repressor</keyword>
    <keyword evidence="11 22" id="KW-0346">Stress response</keyword>
    <keyword evidence="14 22" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 22" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 22" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15 22" id="KW-0834">Unfolded protein response</keyword>
    <keyword evidence="6 22" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="BZIP" evidence="24">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="31"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="168" mass="19003" checksum="D178D4EC04980AD5" modified="2019-09-18" version="1">MAAESLPFSFGALSSWELEAWYEDLQEVLSSDENRGTCVSPPGNKEEESKSFTTLDPASLAWLTEEPGPPEVTHTSQSPCSPESSQSSLAQEEEEEDQGRTRKRKQSGQSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQL2</accession>
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        <lineage>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Pecora</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="276" mass="30730" checksum="522F0FAE150B887C" modified="2019-09-18" version="1">MCIIFFKFDPRPVSKNAYRLILAANRDEFYHRPARAADFWGSNNEVLSGLDMEEGKEGGTWLGISTRGKLAALTNYLQPRLNRNARGRGELVAQFLTSDMDSLSYLKKVSAEGHLYNGFNLIAADLSAEKGDVICYYGNRGEREPVVLAPGTYGLSNALLETPWRKLCFGKQLFLEAVERGRELPRDALVAQLLAVLSNDEAQLPDPAIEAQGGEYVRPILSKYAAVCVRCPDYGTRTSTVILVDADGHVTFTERSMLGSDPTRWETTTHEFRLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRS2</accession>
    <name>A0A4W2CRS2_BOBOX</name>
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        <submittedName>
            <fullName evidence="9">FERM domain containing kindlin 2</fullName>
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        <name evidence="9" type="primary">FERMT2</name>
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        <lineage>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
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        <text evidence="3">Belongs to the kindlin family.</text>
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        <property type="nucleotide sequence ID" value="XM_027554135.1"/>
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        <property type="gene ID" value="ENSBIXG00000003410.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005032364.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:integrin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17181">
        <property type="entry name" value="FERM_F0_KIND2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FERM_F1_KIND2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Fermitin family homolog 2"/>
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    </dbReference>
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        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
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    </dbReference>
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        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FERM_central"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014557.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="680" mass="77874" checksum="38E266089C3D4977" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSZ1</accession>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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    </protein>
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            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046837.1">
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        <property type="gene ID" value="ENSBIXG00000019659.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="573" mass="66265" checksum="CE2165794EC63DBA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVA7</accession>
    <name>A0A4W2CVA7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras association domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RASSF3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CVA7"/>
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        <property type="protein sequence ID" value="ENSBIXP00000015867.1"/>
        <property type="gene ID" value="ENSBIXG00000003694.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005003643.1"/>
        <property type="gene ID" value="ENSBIXG00005000572.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157502"/>
    <dbReference type="OMA" id="YCRDLVH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RA_RASSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ras association domain-containing protein 3"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="RA_dom"/>
    </dbReference>
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        <property type="entry name" value="RASSF1-6"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
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        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738">
        <property type="entry name" value="RASSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16517">
        <property type="entry name" value="Nore1-SARAH"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00788">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
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        <property type="entry name" value="RA"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="8">
        <location>
            <begin position="79"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="9">
        <location>
            <begin position="184"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="236" mass="27206" checksum="4D3BA5B7451B45D4" modified="2019-09-18" version="1">MSSGYSSLEEDSEDFFFTARTSFFRRAPQGKLRTGQQDVEKEKETHNYLSKEEIKEKVHKYNLAVTDKLKMTLNSNGIYTGFIKVQMELCKPSQTAGKPAPSSNGCMNTLHISSTNTVGEVIEALLKKFLVTESPTKFALYKRCHREDQVYACKLSDREHPLYLRLVAGPRTDTLSFVLREHEIGEWEAFSLPELQNFLRILDKEEDEQLQNLKERYAAYRHKLEEALSQAWKPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW40</accession>
    <name>A0A4W2CW40_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">DNA repair protein REV1</fullName>
            <ecNumber evidence="14">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">REV1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW40"/>
    <dbReference type="SMR" id="A0A4W2CW40"/>
    <dbReference type="STRING" id="30522.A0A4W2CW40"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030281.1">
        <property type="protein sequence ID" value="ENSBIXP00000017626.1"/>
        <property type="gene ID" value="ENSBIXG00000021611.1"/>
    </dbReference>
    <dbReference type="OMA" id="IKNGMWM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="BRCT_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PolY_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rev1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19318">
        <property type="entry name" value="Rev1_UBM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000025">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1280:FF:000001">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000001">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000005">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000010">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000005">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000009">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1280">
        <property type="entry name" value="DNA repair protein Rev1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012112">
        <property type="entry name" value="REV1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031991">
        <property type="entry name" value="Rev1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038401">
        <property type="entry name" value="Rev1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047346">
        <property type="entry name" value="Rev1_UBM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1253" mass="137355" checksum="E5409F2039B97D0B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW55</accession>
    <name>A0A4W2CW55_BOBOX</name>
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        <recommendedName>
            <fullName evidence="12">Histamine H3 receptor</fullName>
        </recommendedName>
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            <taxon>Eutheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Histamine_H3_rcpt"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HISTAMINE H3 RECEPTOR"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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        </location>
    </feature>
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        </location>
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        <location>
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            <end position="417"/>
        </location>
    </feature>
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            <begin position="289"/>
            <end position="340"/>
        </location>
    </feature>
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            <end position="304"/>
        </location>
    </feature>
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            <end position="317"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="450" mass="49159" checksum="F99E2D0C402A8AD8" modified="2019-09-18" version="1">MERSPPDGPLNASGALAGEAAAAATGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNNFFLLNLAISDFLVGAFCIPLYVPYVLTGRWPFGRGLCKLWLVVDYLLCTSSVFNIVLISYDRFLSVTRAVSYRAQQGDTRRAVQKMVLVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTPFLSVTFFNLSIYLNIQRRTRVRLDGVREAATTELPPEAQPSPPPAAPSCWGCWQKGCGEAVPLHRYGVGVSEVTPGPEAGEVALGGGSGGGAAASPTSSSGSSSRGTERPRSLKRGSKPSASSASLEKRMKMVSQSITQRFRLSRDKKVAKSLAVIVSIFGLCWAPYTLLMIIRAACHGHCIPDYWYETSFWLLWANSAVNPVLYPLCHYSFRRAFTKLLCPQKLKIQPHSSLEHCWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CX26</accession>
    <name>A0A4W2CX26_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 178 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSBIXP00000017961.1"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="entry name" value="FAM178"/>
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        <property type="entry name" value="PROTEIN FAM178B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="284" mass="30540" checksum="0A026AD59FC4D00F" modified="2019-09-18" version="1">MLSRVKLFSWRGKTWEQPARCPVGPCLPPPLPRVLAGVELPAGGLLLGLWAAKLALCVHSCEPGAGRTVVETIYRLGRPEQGSTALVLLSSLCQLLSLMRPSSLRRYLGPETVLPGQEQQPKASAQLDHKACYLCHSLLTLAGVVVSCQDITPDQWGELQLLCMQLDRHINTHIRESPQAMHQTTLKDLAAQTYIRWQELLAHCQPQVVPPPHSPPGLEGEGSGQLTDQSPQSVCPLCSCQAPGWEMGEGSGGGGDAPLVDRRPSYQGASAQGRQGAVRGTQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXK9</accession>
    <name>A0A4W2CXK9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXK9"/>
    <dbReference type="STRING" id="30522.A0A4W2CXK9"/>
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        <property type="protein sequence ID" value="ENSBIXP00000018200.1"/>
        <property type="gene ID" value="ENSBIXG00000004283.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:phospholipid biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:piRNA processing"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="GPAT/DHAPAT"/>
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        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 2, MITOCHONDRIAL"/>
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        <property type="entry name" value="GPAT_C"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="199"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="794" mass="88160" checksum="DC66FE2ED0C8A71E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXN3</accession>
    <name>A0A4W2CXN3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXN3"/>
    <dbReference type="STRING" id="30522.A0A4W2CXN3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031132.1">
        <property type="protein sequence ID" value="ENSBIXP00000018233.1"/>
        <property type="gene ID" value="ENSBIXG00000004284.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00125">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
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        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="67" mass="7419" checksum="231C15B698FB8039" modified="2019-09-18" version="1">MGIMNSFVNDIFERIAGEALHLAHYNKRSTITSREIQTAVRLLLPGELAKHTVSEGTKGVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D356</accession>
    <name>A0A4W2D356_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acireductone dioxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Acireductone dioxygenase (Fe(2+)-requiring)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Acireductone dioxygenase (Ni(2+)-requiring)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1</fullName>
            <shortName evidence="14">ARD</shortName>
            <shortName evidence="14">ARD'</shortName>
            <shortName evidence="14">Fe-ARD</shortName>
            <shortName evidence="14">MTCBP-1</shortName>
            <shortName evidence="14">Ni-ARD</shortName>
            <ecNumber evidence="14">1.13.11.53</ecNumber>
            <ecNumber evidence="14">1.13.11.54</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ADI1</name>
        <name evidence="14" type="synonym">MTCBP1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. Also down-regulates cell migration mediated by MMP14.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:49016"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="14">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="14">Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with MMP14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 14">Cell membrane</location>
            <topology evidence="4 14">Peripheral membrane protein</topology>
            <orientation evidence="4 14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 14">Nucleus</location>
        </subcellularLocation>
        <text evidence="14">Localizes to the plasma membrane when complexed to MMP14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the acireductone dioxygenase (ARD) family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.53" evidence="14"/>
    <dbReference type="EC" id="1.13.11.54" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D356"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034244.1">
        <property type="protein sequence ID" value="ENSBIXP00000020256.1"/>
        <property type="gene ID" value="ENSBIXG00000023604.1"/>
    </dbReference>
    <dbReference type="OMA" id="NNYIKLM"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00878"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010308">
        <property type="term" value="F:acireductone dioxygenase (Ni2+-requiring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010309">
        <property type="term" value="F:acireductone dioxygenase [iron(II)-requiring] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02232">
        <property type="entry name" value="cupin_ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000031">
        <property type="entry name" value="1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03154">
        <property type="entry name" value="Salvage_MtnD_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004313">
        <property type="entry name" value="ARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027496">
        <property type="entry name" value="ARD_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418:SF0">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03079">
        <property type="entry name" value="ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6 14" id="KW-0533">Nickel</keyword>
    <keyword evidence="13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="186" mass="21746" checksum="F2B4E4F3B4556371" modified="2019-09-18" version="1">MVEAWYMDEAADDPRLPHRAEPARPVGLEQLRRLGVLYWKLDADKYENDPELEKIRKERNYSWVDIITISKDKLPNYEEKIKMFFEEHLHLDEEIRYILDGSGYFDVRDQEDRWIRISMEKGDMITLPAGIYHRFTLDEKLKAQDSERMCEGRKVAPLLAPASLPEGGHDQVLKSLLGETARLAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3N9</accession>
    <name>A0A4W2D3N9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Keratin 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KRT4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3N9"/>
    <dbReference type="STRING" id="30522.A0A4W2D3N9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032076.1">
        <property type="protein sequence ID" value="ENSBIXP00000018813.1"/>
        <property type="gene ID" value="ENSBIXG00000022581.1"/>
    </dbReference>
    <dbReference type="OMA" id="LMQDSVE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF3">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="147"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="151"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="351"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="549" mass="58074" checksum="9E448570AF492F0A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4N2</accession>
    <name>A0A4W2D4N2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <sequence length="955" mass="111504" checksum="103B651D39453D54" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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        </alternativeName>
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            <taxon>Mammalia</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="474" mass="47796" checksum="8CA2CE51F10345C8" modified="2019-09-18" version="1">MMERIRKEMILMERGLHSPTTGKRFSNLSDSAGNAVLEALENSQHPARLSPRLPSAPLHSALGDLPAKGKFEIDTLFNLQHPSSESTVSSEIASAAEGRKKPGHYSEAAAEADMSSDVEVGCSALRSPGGLGAVPLKENNGKGFAESGSVAGTTTSATGSGLGSLHGGGGGGGSGGGTALGGSGSGADQVRRYRTAFTREQIARLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMSWPHPADPSFYTYMMTHAAATGSLPYPFHSHVPLHYYPHVGVTAAAAAAAASGAAAAASSPFATSIRPLDTFRALSHPYSRPELLCSFRHPGLYQTPAAAAGLNSAASAAAAAAAAAAAASSAAAAGAPPSGGSAPCSCLSCHSSQSAAAAAAAAAAALGSRGGGGGSGGGGGSTGAAGGSDFGCSGAAPRSESGFLPYSAAVLSKTAVSPPDQRDEAPLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8Z5</accession>
    <name>A0A4W2D8Z5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Holliday junction recognition protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8Z5"/>
    <dbReference type="STRING" id="30522.A0A4W2D8Z5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037602.1">
        <property type="protein sequence ID" value="ENSBIXP00000022469.1"/>
        <property type="gene ID" value="ENSBIXG00000005167.1"/>
    </dbReference>
    <dbReference type="OMA" id="SFIAHSW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021052">
        <property type="entry name" value="HJURP_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018465">
        <property type="entry name" value="Scm3/HJURP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15992">
        <property type="entry name" value="HOLLIDAY JUNCTION RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15992:SF5">
        <property type="entry name" value="HOLLIDAY JUNCTION RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12346">
        <property type="entry name" value="HJURP_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10384">
        <property type="entry name" value="Scm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Holliday junction recognition protein HJURP central" evidence="5">
        <location>
            <begin position="155"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Holliday junction regulator protein family C-terminal" evidence="6">
        <location>
            <begin position="293"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Holliday junction regulator protein family C-terminal" evidence="6">
        <location>
            <begin position="428"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="67"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="63358" checksum="DDDBE0BD38E630AE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAD8</accession>
    <name>A0A4W2DAD8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAS-associated factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">UBX domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FAF2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis. Involved in stress granule disassembly: associates with ubiquitinated G3BP1 in response to heat shock, thereby promoting interaction between ubiquitinated G3BP1 and VCP, followed by G3BP1 extraction from stress granules and stress granule disassembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with YOD1. Interacts (via N-terminus) with UBQLN2 (via C-terminus). Interacts with PNPLA2 and UBAC2. Interacts with ZFAND2B; probably through VCP. Interacts with LMBR1L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027402724.1">
        <property type="nucleotide sequence ID" value="XM_027546923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAD8"/>
    <dbReference type="SMR" id="A0A4W2DAD8"/>
    <dbReference type="STRING" id="30522.A0A4W2DAD8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035629.1">
        <property type="protein sequence ID" value="ENSBIXP00000021142.1"/>
        <property type="gene ID" value="ENSBIXG00000024285.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033711.1">
        <property type="protein sequence ID" value="ENSBIXP00005020377.1"/>
        <property type="gene ID" value="ENSBIXG00005023506.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895564"/>
    <dbReference type="CTD" id="23197"/>
    <dbReference type="GeneTree" id="ENSGT00940000157197"/>
    <dbReference type="OMA" id="ILIRHQW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02991">
        <property type="entry name" value="UAS_ETEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14414">
        <property type="entry name" value="UBA_FAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16120">
        <property type="entry name" value="UBX_UBXN3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000101">
        <property type="entry name" value="FAS-associated factor 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000066">
        <property type="entry name" value="FAS-associated factor 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000043">
        <property type="entry name" value="Fas-associated factor family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="13">
        <location>
            <begin position="357"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="299"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="303"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="52664" checksum="42979FF2F5E38119" modified="2019-09-18" version="1">MAAPEERDLTQEQTEKLLQFQDLTGIESMDQCRHTLEQHNWNIEAAVQDRLNEQEGVPSVFNPPPSRPLQVNTADHRIYSYVVSRPQPRGLLGWGYYLIMLPFRFTYYTILDIFRFALRFIRPDPRNRVTDPVGDIVSFMHSFEEKYGRAHPVFYLGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRRNLQEEKERKLECLPPEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCLPSEEWPNPPTLQEAGLSHTEVLFVQDLTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCR5</accession>
    <name>A0A4W2DCR5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein beta-7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cardiovascular heat shock protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HSPB7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with C-terminal domain of actin-binding protein 280.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027378547.1">
        <property type="nucleotide sequence ID" value="XM_027522746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCR5"/>
    <dbReference type="STRING" id="30522.A0A4W2DCR5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039585.1">
        <property type="protein sequence ID" value="ENSBIXP00000023827.1"/>
        <property type="gene ID" value="ENSBIXG00000001648.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036165.1">
        <property type="protein sequence ID" value="ENSBIXP00005022035.1"/>
        <property type="gene ID" value="ENSBIXG00005007084.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880506"/>
    <dbReference type="CTD" id="27129"/>
    <dbReference type="GeneTree" id="ENSGT00390000010674"/>
    <dbReference type="OMA" id="CNPYMEK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031005">
        <property type="term" value="F:filamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06479">
        <property type="entry name" value="ACD_HspB7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000020">
        <property type="entry name" value="heat shock protein beta-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037885">
        <property type="entry name" value="ACD_HspB7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907:SF2">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="13">
        <location>
            <begin position="63"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="169" mass="18420" checksum="D3B851FFA03BE0B0" modified="2019-09-18" version="1">MSHRTSSTFRAERSFHSSSSSSSSASHSLPAQDPPMEKALSMFSEDFGGFMRPHSEPLAFPALPTARAAGPGNIKTLGDAYEFAVDVSDFSPEDIIVTTSNNHIEVRAEKLAADGTVMNTFAHKCQLPEDVDPTSVTSALREDGSLTIRARRQPPTEHVQQTFRTEIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DD46</accession>
    <name>A0A4W2DD46_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAT1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DD46"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037258.1">
        <property type="protein sequence ID" value="ENSBIXP00000022225.1"/>
        <property type="gene ID" value="ENSBIXG00000025167.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000011">
        <property type="entry name" value="Methionine aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF8">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M24" evidence="9">
        <location>
            <begin position="213"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="451" mass="49043" checksum="DB18A54009027363" modified="2019-09-18" version="1">MHQNVDATNIGYHAYLTGFFHPTWARGLRESARTAAAARLSWLELARPAAGSPTGPGARAAPPPELPQSAPGAHARSPPAASAPLVFGSAASGRLRRAGRGGARCERVCGHVTDANMAAPSGLHLFVRRGCHRIFSSPLNHIYLHTQSTSQQRRNFFFRRQREISHSIVLPAAVSSAHPVPKHIKKPDYVTTGIVPDWGDSIEVKNEDQIQGLLQACQLARHVLLLAGKSLKVDMTTEEIDALVHQEIISHDAYPSPLGYGGFPKSVCTSVNNVLCHGIPDSRPLQDGDIINIDVTVYYNGYHGDTSETFLVGNVDECGKKLVEVARRCRDEAIAACRAGAPFSVIGNTISQITHQNGLQVCPHFVGHGIGSYFHGHPEIWHHANDSNLLMEEGMAFTIEPIVTEGSPEFKVLEDSWTVVSLDNQRSAQFEHTVLITSRGAQILTKLPYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DE17</accession>
    <name>A0A4W2DE17_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hippocalcin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HPCAL1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411894.1">
        <property type="nucleotide sequence ID" value="XM_027556093.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027411895.1">
        <property type="nucleotide sequence ID" value="XM_027556094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DE17"/>
    <dbReference type="SMR" id="A0A4W2DE17"/>
    <dbReference type="STRING" id="30522.A0A4W2DE17"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047800.1">
        <property type="protein sequence ID" value="ENSBIXP00000017025.1"/>
        <property type="gene ID" value="ENSBIXG00000003993.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049399.1">
        <property type="protein sequence ID" value="ENSBIXP00005018723.1"/>
        <property type="gene ID" value="ENSBIXG00005006887.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901569"/>
    <dbReference type="CTD" id="3241"/>
    <dbReference type="GeneTree" id="ENSGT00940000154645"/>
    <dbReference type="OMA" id="RQHTEFN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000078">
        <property type="entry name" value="Hippocalcin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000072">
        <property type="entry name" value="Hippocalcin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF79">
        <property type="entry name" value="HIPPOCALCIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="193" mass="22285" checksum="1EDCE938DCAD4CA8" modified="2019-09-18" version="1">MGKQNSKLRPEVLQDLRENTEFTDHELQEWYKGFLKDCPTGHLTVDEFKKIYANFFPYGDASKFAEHVFRTFDTNGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISRSEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNNDGKLSLEEFIKGAKSDPSIVRLLQCDPSSASQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DF51</accession>
    <name>A0A4W2DF51_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">Alkaline phosphatase</fullName>
            <ecNumber evidence="33">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">ALPL</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>ADP + H2O = AMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:61437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>AMP + H2O = adenosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29375"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:29376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-phosphocreatine + H2O = creatine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12977"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
            <dbReference type="ChEBI" id="CHEBI:58092"/>
            <dbReference type="EC" id="3.9.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:12978"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:15018"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:24577"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>phosphoethanolamine + H2O = ethanolamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16089"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:58190"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:16090"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20533"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:20534"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="31">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="31">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="31">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="31">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Extracellular vesicle membrane</location>
            <topology evidence="23">Lipid-anchor</topology>
            <topology evidence="23">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Mitochondrion membrane</location>
            <topology evidence="22">Lipid-anchor</topology>
            <topology evidence="22">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 32">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="33"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DF51"/>
    <dbReference type="STRING" id="30522.A0A4W2DF51"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038320.1">
        <property type="protein sequence ID" value="ENSBIXP00000022954.1"/>
        <property type="gene ID" value="ENSBIXG00000002060.1"/>
    </dbReference>
    <dbReference type="OMA" id="YQLMHNV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ADP phosphatase activity"/>
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        <property type="term" value="F:alkaline phosphatase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
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        <property type="term" value="F:phosphoamidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0052732">
        <property type="term" value="F:phosphoethanolamine phosphatase activity"/>
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        <property type="project" value="RHEA"/>
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    <dbReference type="GO" id="GO:0033883">
        <property type="term" value="F:pyridoxal phosphatase activity"/>
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        <property type="project" value="RHEA"/>
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    <dbReference type="GO" id="GO:0031214">
        <property type="term" value="P:biomineral tissue development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF74">
        <property type="entry name" value="ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0091">Biomineralization</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12 33" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15 31 33" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 34" id="KW-0732">Signal</keyword>
    <keyword evidence="13 31 33" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="34">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021497879" description="Alkaline phosphatase" evidence="34">
        <location>
            <begin position="16"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="30">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="337"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="341"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="378"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="379"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00036105"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048784"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048929"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049444"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049526"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="524" mass="57185" checksum="6E49C1A4FDA9C641" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFS3</accession>
    <name>A0A4W2DFS3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="40">ATP-dependent DNA/RNA helicase DHX36</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="42">DEAD/H box polypeptide 36</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="41">MLE-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="46 47">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="46 47" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="46" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="46" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Perikaryon</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A4W2DFS3"/>
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    <dbReference type="STRING" id="30522.A0A4W2DFS3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038398.1">
        <property type="protein sequence ID" value="ENSBIXP00000023007.1"/>
        <property type="gene ID" value="ENSBIXG00000001955.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0010494">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005829">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:dendrite"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G-quadruplex DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
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        <property type="entry name" value="WHD_HA2"/>
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    <keyword evidence="30" id="KW-0007">Acetylation</keyword>
    <keyword evidence="35" id="KW-0010">Activator</keyword>
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    <keyword evidence="23" id="KW-0347">Helicase</keyword>
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    <keyword evidence="26" id="KW-0810">Translation regulation</keyword>
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        <location>
            <begin position="219"/>
            <end position="389"/>
        </location>
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        <location>
            <begin position="479"/>
            <end position="649"/>
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        <location>
            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="16"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022845"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00069921"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00083335"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1010" mass="114826" checksum="8205792C016B1018" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGB7</accession>
    <name>A0A4W2DGB7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 15">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HACD4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3R)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:39159"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:74278"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:45813"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 15"/>
    <dbReference type="RefSeq" id="XP_027404947.1">
        <property type="nucleotide sequence ID" value="XM_027549146.1"/>
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    <dbReference type="STRING" id="30522.A0A4W2DGB7"/>
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        <property type="protein sequence ID" value="ENSBIXP00000017869.1"/>
        <property type="gene ID" value="ENSBIXG00000021784.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036523.1">
        <property type="protein sequence ID" value="ENSBIXP00005022261.1"/>
        <property type="gene ID" value="ENSBIXG00005025173.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896994"/>
    <dbReference type="CTD" id="401494"/>
    <dbReference type="GeneTree" id="ENSGT00530000062962"/>
    <dbReference type="OMA" id="CGHTWIF"/>
    <dbReference type="UniPathway" id="UPA00094"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE 4"/>
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    </dbReference>
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        <property type="entry name" value="PTPLA"/>
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    </dbReference>
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        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
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        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="129"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00007811"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00013122"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022516"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023727"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="231" mass="27589" checksum="2AED4DF92C4718FB" modified="2019-09-18" version="1">MGPVALPTWLQPRYRKNAYLFIYYLIQFCGHSWIFTNMTVRFFSFGKDSMVDTFYAIGLVMQLCQSISLLELLHIYVGIESNHLLPRILQLTERIIVLFMVITSQEEVQEKYVVCVLFIFWNLLDMVRYTYSMLSVIGISYAVLTWFSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTYSTKLPFDLSFYFPYVLKIYLMMLFVGMYFTYNHLYSERRDILRVFPNKKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DHX6</accession>
    <name>A0A4W2DHX6_BOBOX</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="AlphaFoldDB" id="A0A4W2DHX6"/>
    <dbReference type="STRING" id="30522.A0A4W2DHX6"/>
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        <property type="protein sequence ID" value="ENSBIXP00000018467.1"/>
        <property type="gene ID" value="ENSBIXG00000022314.1"/>
    </dbReference>
    <dbReference type="OMA" id="IEIMEMA"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="POT_fam"/>
    </dbReference>
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        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta knot"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="274" mass="30852" checksum="8C5663D7AC05D503" modified="2019-09-18" version="1">MAVGMILACLGFAVAAAVEIKINEMATCQPNSQEIFLQVLNLADDEITVTVQGDKNNTLLAESIKSFQNMPHYSKLHLKTKSQNFHFQLKYHNVSVYTEHSVEEKMWYTLIIREDGESISSMMVKDKENKTTDGMTAMRFVNALHEEVNVSLGTDASLIVDEDYGVSAYRTVQRGEYPVVHCRTKNEDFSLNLGLLDFGAAYLFVITNRTSQGPQAWKMEYIPANKMSVAWQLPQYALVTAGEVMFSVTGLEFSYSQVSLFFFFNINLFILIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DI16</accession>
    <name>A0A4W2DI16_BOBOX</name>
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        <submittedName>
            <fullName evidence="12">Chromosome 1 open reading frame 43</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="synonym">C1orf43</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
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    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">General regulator of phagocytosis. Required to uptake Gram negative bacterium by macrophages.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027388364.1">
        <property type="nucleotide sequence ID" value="XM_027532563.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSBIXT00000039492.1">
        <property type="protein sequence ID" value="ENSBIXP00000023763.1"/>
        <property type="gene ID" value="ENSBIXG00000005415.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011009.1">
        <property type="protein sequence ID" value="ENSBIXP00005003067.1"/>
        <property type="gene ID" value="ENSBIXG00005009324.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886384"/>
    <dbReference type="CTD" id="25912"/>
    <dbReference type="GeneTree" id="ENSGT00510000047366"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C1orf43"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21425:SF2">
        <property type="entry name" value="PROTEIN C1ORF43"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00002620"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="219" mass="24704" checksum="A1B7D868761CFD6D" modified="2019-09-18" version="1">MASGSNWLSGVNVVLVMAYGSLDLKEEIDIRLSKVQDIKYEPQLLADDDARLLQLETQGNQNCYNYLYRMKALDAIRASEIPFHAEGRHPHSLMGKNFRSYLLDLRNTSTPFKGVRKALIDTLLDGYETARYGTGVFGLSEYLRYQEALSELATVVKARSGSSQRQHQSAAKDLTQSPEVSPTTIQVTYLPSSQKSKRAKHFLELKSFKDNYNTLESTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DI91</accession>
    <name>A0A4W2DI91_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras-related GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DI91"/>
    <dbReference type="SMR" id="A0A4W2DI91"/>
    <dbReference type="STRING" id="30522.A0A4W2DI91"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042858.1">
        <property type="protein sequence ID" value="ENSBIXP00000025930.1"/>
        <property type="gene ID" value="ENSBIXG00000028030.1"/>
    </dbReference>
    <dbReference type="OMA" id="HEIFEVR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000001">
        <property type="entry name" value="Ras-related GTP-binding protein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000226">
        <property type="entry name" value="Ras-related GTP-binding protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF6">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="399" mass="44233" checksum="D4326670732CC033" modified="2019-09-18" version="1">MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGAGAGAGGGCGPGGADSSKPRILLMGLRRSGKSSIQKVVFHKMSPNEILFLESTNKIYKDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFNVVSKIYIATDSSPVDMQSYELCCDMIDVVTDVSCIYGLKEDGSGSAYDKESMAIIKLNNTAVLYLKEVTKFLALVCILREESFERRGLIDYNFHCFRKAIHEVFEVGVTSHRSCSHQTSAPSLKALTHNGTPRNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJ69</accession>
    <name>A0A4W2DJ69_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrin-binding sialoprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Bone sialoprotein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Bone sialoprotein II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Cell-binding sialoprotein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3).</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with integrins; the interaction promotes cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJ69"/>
    <dbReference type="SMR" id="A0A4W2DJ69"/>
    <dbReference type="STRING" id="30522.A0A4W2DJ69"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051411.1">
        <property type="protein sequence ID" value="ENSBIXP00000024172.1"/>
        <property type="gene ID" value="ENSBIXG00000001437.1"/>
    </dbReference>
    <dbReference type="OMA" id="HAYFYPH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008412">
        <property type="entry name" value="IBSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10345">
        <property type="entry name" value="BONE SIALOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10345:SF0">
        <property type="entry name" value="BONE SIALOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05432">
        <property type="entry name" value="BSP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0091">Biomineralization</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021503437" description="Integrin-binding sialoprotein" evidence="16">
        <location>
            <begin position="17"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="61"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="310" mass="34101" checksum="B5BD8F61BAD88261" modified="2019-09-18" version="1">MKTVLILLSILGMACALSMKNLNRRAKLEDSEENGVFKYRPQYYVYKHGYFYPALKRFAVQSSSDSSEENGNGDSSEEEEEEEETSNEEGNNGGNEDSDENEDEESEAENTTLSTTTLGYGEITPGTGDIGLAAIWLPRKAGATGKKATKEDESDEEEEEEEEEENEAEVDDNEQGINGTSSNSTEVDNGHGSSGGDNGEEDGEEESVTEANTEGITVAGETTTSPNGGFKPTTPHQEVYGTTPPPFGKITTPGEYEQTGTNEYDNGYEIYESENGDPRGDNYRAYEDEYSYYKGRGYDSYDGQDYYSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DK20</accession>
    <name>A0A4W2DK20_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Actin binding LIM protein family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABLIM3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>IDENTIFICATION</scope>
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</entry>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSBIXT00000052072.1">
        <property type="protein sequence ID" value="ENSBIXP00000025238.1"/>
        <property type="gene ID" value="ENSBIXG00000005741.1"/>
    </dbReference>
    <dbReference type="OMA" id="VPNRINT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1820:FF:000002">
        <property type="entry name" value="LINE-1 retrotransposable element ORF1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.390">
        <property type="entry name" value="L1 transposable element, trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043636">
        <property type="entry name" value="L1_RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035301">
        <property type="entry name" value="L1_trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17489">
        <property type="entry name" value="Tnp_22_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02994">
        <property type="entry name" value="Transposase_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L1 transposable element trimerization" evidence="5">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="L1 transposable element RRM" evidence="4">
        <location>
            <begin position="145"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="42"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="30494" checksum="B3A493E61EE545B3" modified="2019-09-18" version="1">MKRQRNTQQIKEQDKCSPNQTKEEEIGNLPDKEFRIMIVKLIQNLEIKMESQINSLETKIEKMQERFNKDLEEIKESQYKMKNAINEIKNTLEATNSRITEAEDRISELEDRMVEINESERIKEKRIKRNEDNLRDLQDNIKGYNIRIIGVPEEEDKKKDHEKILEEIIVENFPKMGKEIITQVQETQRVPNRINTRRNTPRHILIKLTKIKHKEQILKAAREKQQITHKGIPIRLIADLSIETLQARREWQDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNN8</accession>
    <name>A0A4W2DNN8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Integrin alpha-9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Integrin alpha-9/beta-1 (ITGA9:ITGB1) is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. ITGA9:ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion. Integrin ITGA9:ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer of an alpha and a beta subunit. Alpha-9 (ITGA9) associates with beta-1 (ITGB1). Integrin ITGA9:ITGB1 interacts with FBLN5 (via N-terminus). Integrin ITGA9:ITGB1 interacts with SPP1/OPN (via N-terminus). Integrin ITGA9:ITGB1 interacts with TNC/TNFN3 (via the 3rd Fibronectin type-III domain). Integrin ITGA9:ITGB1 interacts with SVEP1/polydom (via Sushi domain 21); thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNN8"/>
    <dbReference type="STRING" id="30522.A0A4W2DNN8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011414.1">
        <property type="protein sequence ID" value="ENSBIXP00000028073.1"/>
        <property type="gene ID" value="ENSBIXG00000009139.1"/>
    </dbReference>
    <dbReference type="OMA" id="MMRKGMA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034679">
        <property type="term" value="C:integrin alpha9-beta1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000007">
        <property type="entry name" value="Integrin subunit alpha 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000002">
        <property type="entry name" value="Integrin, alpha 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000006">
        <property type="entry name" value="Integrin, alpha 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000013">
        <property type="entry name" value="Integrin, alpha 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1530:FF:000005">
        <property type="entry name" value="Integrin, alpha 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF69">
        <property type="entry name" value="INTEGRIN ALPHA-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 19" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021499346" description="Integrin alpha-9" evidence="19">
        <location>
            <begin position="30"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="978"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="18">
        <location>
            <begin position="35"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="18">
        <location>
            <begin position="290"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="18">
        <location>
            <begin position="351"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="18">
        <location>
            <begin position="413"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="20">
        <location>
            <begin position="459"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="21">
        <location>
            <begin position="620"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="22">
        <location>
            <begin position="772"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1034" mass="114247" checksum="159F8A1D4B231BDB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DPE9</accession>
    <name>A0A4W2DPE9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF206">
        <property type="entry name" value="FORKHEAD BOX PROTEIN Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="107"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="107"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="384" mass="38702" checksum="DF0279054FDB04D4" modified="2019-09-18" version="1">MKLEVFGPRAGLGDKPGSDLEGAGGSDAPSPLSAAGDDSLGSDGDCAANSPAAGSGERSAGGGPGAEEAGAAGAAGATAGPRAGGAGSGGAGGGEGARGKPYTRRPKPPYSYIALIAMAIRDSAGGRLTLAEINEYLMGKFPFFRGSYTGWRNSVRHNLSLNDCFVKVLRDPSRPWGKDNYWMLNPNSEYTFADGVFRRRRKRLSHRAAAPGPALRPPDGAPPPPAPAAAAPASPRARSPARPEGRASPAGRFSGPFAIDSILSKPFRSRRAGDAGPGARPPWGAAPCPPPPAYPALLQAAPAGALLPLCAFGAAEPPGLLARGAEAPPLLLAPLAAPAPAKPLRGPAAGAHLYCPVRLPDALLAASARAPGPHLPNRPETLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUT8</accession>
    <name>A0A4W2DUT8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUT8"/>
    <dbReference type="STRING" id="30522.A0A4W2DUT8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004095.1">
        <property type="protein sequence ID" value="ENSBIXP00000030416.1"/>
        <property type="gene ID" value="ENSBIXG00000012385.1"/>
    </dbReference>
    <dbReference type="OMA" id="CLRYLGC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF530">
        <property type="entry name" value="MSTPROX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="7">
        <location>
            <begin position="21"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="408" mass="44951" checksum="BCE7684909918267" modified="2019-09-18" version="1">MGSCAGEEGVAPPIPPLRPCDQFHLFVSYSSVDAVWTHRLTGRLEAELPGLKVCLHERDFTPGRNVLENMAGCIQQSQKVLLVLSEDFVQSRWCLLEADLSLVGSCLERKPVLPVLLRPCRVPLHLSHLTYLEATDGRFFQKLVQLLCTPSQHLAHPPALRSAPALYSGKALLTLDCINRDSLPSWKVGSFSTLAVPDPLKEVLEDPELYKQAVGILNGVRSPRSCLRYLGCRIILAIVLILLSVVSVFLPLVLGLQELQPARRFLVISANAFFCPFFMILSVNTLCWLRRSSKKTLRELAGRVGEANLLLAPHSVLMGCETKNKLYFVYVLLGDCKQTFLGAPDGEAAFQEAILRFASSYACCLAHSHFPHWEEAAGAPGHLEVGPCFCQFVSLQLRRLGGVGVSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUW0</accession>
    <name>A0A4W2DUW0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine zipper putative tumor suppressor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein LAPSER1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">LZTS2</name>
        <name evidence="9" type="synonym">LAPSER1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Negative regulator of katanin-mediated microtubule severing and release from the centrosome. Required for central spindle formation and the completion of cytokinesis. May negatively regulate axonal outgrowth by preventing the formation of microtubule bundles that are necessary for transport within the elongating axon. Negative regulator of the Wnt signaling pathway. Represses beta-catenin-mediated transcriptional activation by promoting the nuclear exclusion of beta-catenin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with KATNB1. Also interacts with CTNNB1, gamma-tubulin and KIF23.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cytoskeleton</location>
            <location evidence="9">Microtubule organizing center</location>
            <location evidence="9">Centrosome</location>
        </subcellularLocation>
        <text evidence="9">Localized to the centrosome throughout the cell cycle. Localized to the midbody in cells undergoing cytokinesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the LZTS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUW0"/>
    <dbReference type="STRING" id="30522.A0A4W2DUW0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004030.1">
        <property type="protein sequence ID" value="ENSBIXP00000030441.1"/>
        <property type="gene ID" value="ENSBIXG00000012416.1"/>
    </dbReference>
    <dbReference type="OMA" id="LQHNYVQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051168">
        <property type="term" value="P:nuclear export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051255">
        <property type="term" value="P:spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03026">
        <property type="entry name" value="LZTS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028597">
        <property type="entry name" value="LZTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF4">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06818">
        <property type="entry name" value="Fez1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="378"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="566"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear export signal" evidence="9">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="241"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="667" mass="72524" checksum="68ECDD9A05F29FBC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWH8</accession>
    <name>A0A4W2DWH8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Phosphatidylinositol 4-kinase type 2</fullName>
            <ecNumber evidence="29">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">PI4K2A</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 29">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Golgi apparatus</location>
            <location evidence="28">trans-Golgi network membrane</location>
            <topology evidence="28">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Membrane</location>
            <topology evidence="29">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Presynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Synapse</location>
            <location evidence="26">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 29">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="29"/>
    <dbReference type="RefSeq" id="XP_027385335.1">
        <property type="nucleotide sequence ID" value="XM_027529534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWH8"/>
    <dbReference type="SMR" id="A0A4W2DWH8"/>
    <dbReference type="STRING" id="30522.A0A4W2DWH8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003333.1">
        <property type="protein sequence ID" value="ENSBIXP00000030700.1"/>
        <property type="gene ID" value="ENSBIXG00000012748.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009974.1">
        <property type="protein sequence ID" value="ENSBIXP00005029733.1"/>
        <property type="gene ID" value="ENSBIXG00005006986.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884689"/>
    <dbReference type="CTD" id="55361"/>
    <dbReference type="GeneTree" id="ENSGT00390000010434"/>
    <dbReference type="OMA" id="IKCDCPL"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
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    </dbReference>
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        <property type="term" value="C:glutamatergic synapse"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:presynaptic active zone"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="24" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="21 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0770">Synapse</keyword>
    <keyword evidence="11" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="12 29" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="31">
        <location>
            <begin position="124"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00037864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="479" mass="54084" checksum="921F79A639E8B749" modified="2019-09-18" version="1">MDETSPLVSPERAQPPDYTFPSGSSAHFPQVPGGAVRVAAAAGPVSSPPGSPGHDRERQPLLDRARGAAAQGQTQTVAAQAQALAAQAAAAAHAAQAHRERNEFPEDPEFEAVVRQAELAIERGIFPERIYQGSSGSYFVKDPQGKIIAVFKPKNEEPYGQLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVESYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDSSNSRDTDWVVVKEPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFVKDLEEDLYELFKKDPGFDRGQFHKQIAVMRGQILNLTQALKDNKCPLHLVQMPPVIVETARSHQRSSSESYTQSFQSRKPFFSWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWI0</accession>
    <name>A0A4W2DWI0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clusterin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the clusterin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWI0"/>
    <dbReference type="STRING" id="30522.A0A4W2DWI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010477.1">
        <property type="protein sequence ID" value="ENSBIXP00000028381.1"/>
        <property type="gene ID" value="ENSBIXG00000009527.1"/>
    </dbReference>
    <dbReference type="OMA" id="YLSEDCP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000753">
        <property type="entry name" value="Clusterin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016015">
        <property type="entry name" value="Clusterin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016014">
        <property type="entry name" value="Clusterin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970:SF2">
        <property type="entry name" value="CLUSTERIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01093">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00035">
        <property type="entry name" value="CLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00030">
        <property type="entry name" value="CLb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021196138" description="Clusterin" evidence="10">
        <location>
            <begin position="25"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin N-terminal" evidence="11">
        <location>
            <begin position="30"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin C-terminal" evidence="12">
        <location>
            <begin position="242"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="67"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010069"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000629"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="469" mass="54270" checksum="BCBE506CABEADFB8" modified="2019-09-18" version="1">HGGNMKPPILVFIVYLLQLRDCQCAPTGKDRTSIREDPKGFSKAGEIDVDEEVKKALIGMKQMKILMERREEEHSKLMRTLKKCREEKQEALKLMNEVQEHLEEEERLCQVSLMGSWDECKSCLESDCMRFYTTCQSSWSSMKSTIERVFRKIYQFLFPFHEDDEKELPVGEKFTEEDVQLMQIENVFSQLTVDVGFLYNMSFHVFKQMQQEFDLAFQSYFMSDTDSMEPYFFPAFSKEPAKKAHPMQSWDIPSFFQLFCNFSLSVYQSVSATVTEMLKAIEDLSKQDKDSAHGGPSSTTWPVRGRGLCGEPGQNSSECLQFHARCQKCQDYLWADCPAVPELYTKADEALELVNISNQQYAQVLQMTQHHLEDTTYLMEKMREQFGWVTELASQTPGSENIFSFIKVVPGVHEGNFSKQDEKMIDISILPSSNFTLTIPLEESAESSDFISYMLAKAVQHFKEHFKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E029</accession>
    <name>A0A4W2E029_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E029"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017545.1">
        <property type="protein sequence ID" value="ENSBIXP00000032456.1"/>
        <property type="gene ID" value="ENSBIXG00000015061.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="96"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="277"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="384" mass="44138" checksum="CCFC1AA0361BAB22" modified="2019-09-18" version="1">MGSIKDRNSVDLTEAEDTKKRWQEYIEELYKKNLHNPDNHDGVITHLEPDIQEWEVKWALESITTNKASGGDGIPGELFQILKDDDVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISYTRKVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANICWIIKTAREFQKNIYFCFINYTKAFNCVDHNKLWNILQEMGIPDHLTCLSRNLYAGQEAIVRTGHGTTDWLQIGKGVFQGCILSSCLFNLYAQYIKRNTGLHEAQTGIKTARRNIKNLRCADDTIVIAESEEELKSLLVKVKEESEKAGLKLNIQKTKIMASGSITSWQTDGKQWKQWLTLFFGAPKSLQMVIAAMKLKDAYSLEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5W5</accession>
    <name>A0A4W2E5W5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Gem-associated protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GEMIN7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts with GEMIN6; the interaction is direct. Interacts with STRAP/UNRIP; the interaction is direct. Interacts with GEMIN8; the interaction is direct. Interacts with SNRPB, SNRPD2, SNRPD3 and SNRPE; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Gem</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the gemin-7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371299.1">
        <property type="nucleotide sequence ID" value="XM_027515498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5W5"/>
    <dbReference type="SMR" id="A0A4W2E5W5"/>
    <dbReference type="STRING" id="30522.A0A4W2E5W5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021960.1">
        <property type="protein sequence ID" value="ENSBIXP00000034345.1"/>
        <property type="gene ID" value="ENSBIXG00000017285.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030437.1">
        <property type="protein sequence ID" value="ENSBIXP00005018198.1"/>
        <property type="gene ID" value="ENSBIXG00005021602.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876362"/>
    <dbReference type="CTD" id="79760"/>
    <dbReference type="GeneTree" id="ENSGT00390000018039"/>
    <dbReference type="OMA" id="CADIISY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097504">
        <property type="term" value="C:Gemini of Cajal bodies"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11677">
        <property type="entry name" value="Gemin7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000040">
        <property type="entry name" value="Gem-associated protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020338">
        <property type="entry name" value="SMN_gemin7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14679">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14679:SF1">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11095">
        <property type="entry name" value="Gemin7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51938">
        <property type="entry name" value="SUZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUZ-C" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Sm" evidence="15">
        <location>
            <begin position="59"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="125" mass="13851" checksum="091367563B26A9FB" modified="2019-09-18" version="1">MQTPLATPVPVLRLPRGPDGSNRGFAPDGRRAPPKPEVPEPPESRESWEQQARASLRERYLRSLLAMVGRPVCFTLHEGVQVIAHFGATDLDVANFYVSQLQTPIGIQAEALLRCSDIISYTFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E622</accession>
    <name>A0A4W2E622_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA pseudouridine(38/39) synthase</fullName>
            <ecNumber evidence="8">5.4.99.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA pseudouridine synthase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA pseudouridylate synthase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA-uridine isomerase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PUS3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>uridine(38/39) in tRNA = pseudouridine(38/39) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42564"/>
            <dbReference type="Rhea" id="RHEA-COMP:10117"/>
            <dbReference type="Rhea" id="RHEA-COMP:10118"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.45"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.45" evidence="8"/>
    <dbReference type="RefSeq" id="XP_027387416.1">
        <property type="nucleotide sequence ID" value="XM_027531615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E622"/>
    <dbReference type="SMR" id="A0A4W2E622"/>
    <dbReference type="STRING" id="30522.A0A4W2E622"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014317.1">
        <property type="protein sequence ID" value="ENSBIXP00000027155.1"/>
        <property type="gene ID" value="ENSBIXG00000028696.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052440.1">
        <property type="protein sequence ID" value="ENSBIXP00005043388.1"/>
        <property type="gene ID" value="ENSBIXG00005027045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885860"/>
    <dbReference type="CTD" id="83480"/>
    <dbReference type="GeneTree" id="ENSGT00950000183160"/>
    <dbReference type="OMA" id="YFGWEYN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160154">
        <property type="term" value="F:tRNA pseudouridine(38/39) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000007">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000005">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="14">
        <location>
            <begin position="212"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="481" mass="55634" checksum="D2956FB95D8CCC78" modified="2019-09-18" version="1">MAENDVDRIQTEKLLKRVQELEQEVKRLKKEQANNKDSNIRENSSGAGGKPKRAFDFSAHGQRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTRLVENRQTSNYHRCGRTDKGVSAFGQVISLDLRSHIPKGRDSEHFNLKNEVNDVATEIRYTHILNRVLPPDIRVLAWAPVETSFSARFSCLERTYRYFFPRANLDIVTMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQRVGQNLGEEGWQEPFQLCQFEVTGQAFLYHQVRCMMAVLFLIGQGMEKPEVIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENIKWIYDREVQEFNVTHLQQLWANHAVKTQMLYSMLQGLDSVALPCGTGPKMDGMIEWRNVKPSVTKQTSAFVEGVKMRTYKPLMDRPKCQGLESRIQHFVRRGRIEHPHLFHEEETKAKRDCSDTLEEENTVFEKPTKRICVDTELKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6E8</accession>
    <name>A0A4W2E6E8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MYB binding protein 1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MYBBP1A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYBBP1A family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2E6E8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017257.1">
        <property type="protein sequence ID" value="ENSBIXP00000032334.1"/>
        <property type="gene ID" value="ENSBIXG00000014873.1"/>
    </dbReference>
    <dbReference type="OMA" id="VWKHDDP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="719"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="952"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1163"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1227"/>
            <end position="1240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1353" mass="152037" checksum="C0CC44174A2406DE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6W3</accession>
    <name>A0A4W2E6W3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Achaete-scute family bHLH transcription factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ASCL3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027417946.1">
        <property type="nucleotide sequence ID" value="XM_027562145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6W3"/>
    <dbReference type="STRING" id="30522.A0A4W2E6W3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023777.1">
        <property type="protein sequence ID" value="ENSBIXP00000035127.1"/>
        <property type="gene ID" value="ENSBIXG00000018323.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023552.1">
        <property type="protein sequence ID" value="ENSBIXP00005036240.1"/>
        <property type="gene ID" value="ENSBIXG00005017718.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905167"/>
    <dbReference type="CTD" id="56676"/>
    <dbReference type="GeneTree" id="ENSGT00940000162850"/>
    <dbReference type="OMA" id="MMDNRSY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007431">
        <property type="term" value="P:salivary gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070654">
        <property type="term" value="P:sensory epithelium regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001894">
        <property type="term" value="P:tissue homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19745">
        <property type="entry name" value="bHLH_TS_ASCL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000038">
        <property type="entry name" value="achaete-scute homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF108">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="93"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="181" mass="21101" checksum="8FA65FE3C91DB8DD" modified="2019-09-18" version="1">MMDGRSYSNMPDQLPVFTDSSHLPLTRSFYLDPMVTFHLYPEGPVPSPYSENLPLLPFSSDSLIMENYGEPCAFSFPMPYPNYRRCEYSYGPAFIRKRNERERQRVKCVNEGYAQLRHHLPEEYLEKRLSKVETLRAAIKYINYLQSLLYPDEAETKNSPSKVSSIMATTTHHSDSIFRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E762</accession>
    <name>A0A4W2E762_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nesprin-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">KASH domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nuclear envelope spectrin repeat protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SYNE3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus outer membrane</location>
            <topology evidence="10">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E762"/>
    <dbReference type="STRING" id="30522.A0A4W2E762"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016244.1">
        <property type="protein sequence ID" value="ENSBIXP00000031905.1"/>
        <property type="gene ID" value="ENSBIXG00000014351.1"/>
    </dbReference>
    <dbReference type="OMA" id="ACCLEDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000237">
        <property type="entry name" value="Spectrin repeat containing nuclear envelope family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000417">
        <property type="entry name" value="Spectrin repeat containing nuclear envelope family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000247">
        <property type="entry name" value="Spectrin repeat-containing, nuclear envelope family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057932">
        <property type="entry name" value="Spectrin_SYNE1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057933">
        <property type="entry name" value="SYNE3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF2">
        <property type="entry name" value="NESPRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25803">
        <property type="entry name" value="Spectrin_SYNE1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25804">
        <property type="entry name" value="SYNE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="15">
        <location>
            <begin position="1"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1037"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="15">
        <location>
            <begin position="1058"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="19">
        <location>
            <begin position="1028"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="63"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="405"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1086" mass="123519" checksum="FB7007795E72D9E4" modified="2019-09-18" version="1"> </sequence>
</entry>
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            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSBIXG00000001742.1"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Human Immunodeficiency Virus Type 1 Capsid Protein"/>
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    </dbReference>
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        <property type="entry name" value="Integrase, C-terminal domain superfamily, retroviral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.490">
        <property type="entry name" value="Retroviral GAG p10 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="B_retro_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038124">
        <property type="entry name" value="B_retro_matrix_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045345">
        <property type="entry name" value="Gag_p24_C"/>
    </dbReference>
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        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036862">
        <property type="entry name" value="Integrase_C_dom_sf_retrovir"/>
    </dbReference>
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        <property type="entry name" value="Integrase_C_retrovir"/>
    </dbReference>
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        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008916">
        <property type="entry name" value="Retrov_capsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008919">
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    </dbReference>
    <dbReference type="InterPro" id="IPR010999">
        <property type="entry name" value="Retrovr_matrix"/>
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    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41694">
        <property type="entry name" value="ENDOGENOUS RETROVIRUS GROUP K MEMBER POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41694:SF3">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19317">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00552">
        <property type="entry name" value="IN_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02022">
        <property type="entry name" value="Integrase_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50122">
        <property type="entry name" value="DNA-binding domain of retroviral integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46919">
        <property type="entry name" value="N-terminal Zn binding domain of HIV integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47836">
        <property type="entry name" value="Retroviral matrix proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47943">
        <property type="entry name" value="Retrovirus capsid protein, N-terminal core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51027">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50876">
        <property type="entry name" value="ZF_INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNase H type-1" evidence="15">
        <location>
            <begin position="603"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase-type" evidence="14">
        <location>
            <begin position="735"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="16">
        <location>
            <begin position="789"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase-type" evidence="17">
        <location>
            <begin position="953"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Integrase-type" evidence="12">
        <location>
            <begin position="953"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50876"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114048" checksum="09B2B0522790E6C4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E916</accession>
    <name>A0A4W2E916_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E916"/>
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        <property type="gene ID" value="ENSBIXG00000019248.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="42711" checksum="AD4E161AEA1D136A" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLNCLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIARRNINNLRYADDTILMAESEEELKSLLLKVKVESEKVGLKLNIQKAKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRCLLLGRKVMTNLDSIFKSRDFTLPTKVCLVKAMVFPVVMYGCESWTVKKAERRRINAFKLWCWIRLLRVPWTARRCNQPILKEINPGCSLKGMMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRGQQRMRWLDDITVSMDVSLSELRELVMDREAWPAGIHGVAKNRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ED64</accession>
    <name>A0A4W2ED64_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSBIXT00000020894.1">
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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    </dbReference>
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            <end position="338"/>
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            <end position="86"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="467" mass="54003" checksum="28501960A1E1549F" modified="2019-09-18" version="1">MKLSHARGATQDGWVTVERSDRMWSTGEGNGQPLQYSCLENPMNSMKRQNDRILKEELPRSVGAQYATGDQWRNNSRKNEGMEPKQKEYPAVDVTGNKVRCYKEQYCIGTWNVRFMNQSKLEVVKQEMARVNVDILGINKLKWTGMGEFNSGDHYIYYCGQESLRRNGIVTMVNKRARNAVLGCNLRKDRMISVCFQGKLFSITVIQVYAPTSNTEEAEVERFYEDLQDLLELTPKKDVLFIIGDWNAKVGSQETPGVTGKFGLGIQNEAGQRLIKFCQENALVIANTLFQQHKRRLYTWTSPDGQHQNLTDYILLQRWRNSIQSIKTRPGADCGSDHELLIVKFRLKLKKVGKTTRPFRYDLNQIPYDYTVEVRNRFKGLDLIDRVPDELWTEVRDVVQETGIKTIPMEKKCKKAKWLSGEALQIAVKRREAKSKGEKERYKHLNAEFQRIARRDKKAGKQQNGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ED71</accession>
    <name>A0A4W2ED71_BOBOX</name>
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        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC113904623</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ED71"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023172.1">
        <property type="protein sequence ID" value="ENSBIXP00000034877.1"/>
        <property type="gene ID" value="ENSBIXG00000017978.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="332" mass="37051" checksum="A72A087906A076CF" modified="2019-09-18" version="1">GSYFIDSSFLIAIGHSHTAQITPMKNSTVVTEFILAGITDDPQLQIPLFLVFTLIYLLTLVGNLGVITLILLDSRLHTPMYIFLSQLSLMDFAYSTAVTPKVMAGFLTGDKVISYHACAAQFFFFAVFLIMETFLLASMAYDHHAAVCQPLHYTNIMTPRVCASMVVGSYVLSFLEASVHTWNIFSLSFCRSRVIDHFFCDAIPLLALSCSDNKRSEIVFFILAGFNIISSVLIILISYLFIFVTILRVRSSEGHQKAFSTCASHLTSVSIFYGTGAFMYLQPGSRHSMSTDKMASVFYAIVIPMLNPLIYSLRNKEVKSAFHRLCGKQHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEU6</accession>
    <name>A0A4W2EEU6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Maestro heat like repeat family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MROH1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2EEU6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004152.1">
        <property type="protein sequence ID" value="ENSBIXP00000030404.1"/>
        <property type="gene ID" value="ENSBIXG00000012364.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055406">
        <property type="entry name" value="HEAT_Maestro"/>
    </dbReference>
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        <property type="entry name" value="HEAT_MROH2B-like"/>
    </dbReference>
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        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
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        <property type="entry name" value="Maestro-like_HEAT"/>
    </dbReference>
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        <property type="entry name" value="Maestro_heat-like_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056282">
        <property type="entry name" value="MROH2B-like_N_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120:SF44">
        <property type="entry name" value="MAESTRO HEAT-LIKE REPEAT-CONTAINING PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120">
        <property type="entry name" value="MAESTRO-RELATED HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21047">
        <property type="entry name" value="HEAT_Maestro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23210">
        <property type="entry name" value="HEAT_Maestro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HEAT_MROH2B_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HEAT_MROH2B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MROH2B-like N-terminal HEAT-repeats" evidence="6">
        <location>
            <begin position="177"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MROH2B-like HEAT-repeats" evidence="5">
        <location>
            <begin position="399"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="Maestro-like HEAT-repeats" evidence="4">
        <location>
            <begin position="1090"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="domain" description="Maestro/Maestro-like HEAT-repeats" evidence="7">
        <location>
            <begin position="1520"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="1767"/>
            <end position="1797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1797" mass="197791" checksum="F4B6F186D74A715D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EF82</accession>
    <name>A0A4W2EF82_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin carboxyl-terminal hydrolase MINDY</fullName>
            <ecNumber evidence="12">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MINDY3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 12">Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with COPS5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the MINDY deubiquitinase family. FAM188 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027415660.1">
        <property type="nucleotide sequence ID" value="XM_027559859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF82"/>
    <dbReference type="SMR" id="A0A4W2EF82"/>
    <dbReference type="STRING" id="30522.A0A4W2EF82"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022856.1">
        <property type="protein sequence ID" value="ENSBIXP00000034731.1"/>
        <property type="gene ID" value="ENSBIXG00000007150.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038079.1">
        <property type="protein sequence ID" value="ENSBIXP00005042622.1"/>
        <property type="gene ID" value="ENSBIXG00005004454.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903545"/>
    <dbReference type="CTD" id="80013"/>
    <dbReference type="GeneTree" id="ENSGT00940000155958"/>
    <dbReference type="OMA" id="VLQTKWP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000315">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase MINDY-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025257">
        <property type="entry name" value="MINDY-3/4_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039785">
        <property type="entry name" value="MINY3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13898">
        <property type="entry name" value="MINDY-3_4_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01174">
        <property type="entry name" value="DUF4205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Deubiquitinating enzyme MINDY-3/4 conserved" evidence="13">
        <location>
            <begin position="9"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="49678" checksum="7C0FF05D75F0AC66" modified="2019-09-18" version="1">MSELTKELMELVWGTNSSPGLSDTIFCRWTQGFVFSESEGSALEQFEGGPCAVIAPVQAFLLKKLLFSSEKSSWRDCPEEERKELLCHTLCDILESAGCDNSGSYCLVSWLRGKTTEETASLSGSPAQSSCQVEHSSALAVEELGFERFHALIQKRSFRSLAELRDAVLDQYSMWGNKFGVLLFLYSVLLTKGIENIKNEIEDSSEPLIDPVYGHGSQSLINLLLTGHAVSNVWDGDRECSGMKLLGIHEQAAVGFLTLMEALRYCKVGSYLKSPKFPIWIVGSETHLTVFFAKDMALVAPEAPSEQARRVFQTYDPEDNGFIPDSLLEDVMKALDLVSDPEYINLMKNKLDPEGLGIILLGPFLQEFFPDQGSSGPESFTVYHYNGLKQSNYNEKVMYVEGTAVVMGFEDPLLQTDDTPIKRCLQTKWPYIELLWTTDRSPSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGL8</accession>
    <name>A0A4W2EGL8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c oxidase assembly factor 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COA8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGL8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001239.1">
        <property type="protein sequence ID" value="ENSBIXP00000031392.1"/>
        <property type="gene ID" value="ENSBIXG00000001404.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018796">
        <property type="entry name" value="COA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107">
        <property type="entry name" value="APOPTOGENIC PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10231">
        <property type="entry name" value="COA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="257" mass="29631" checksum="8C17D77D3CC59A30" modified="2019-09-18" version="1">MLGQLPCAAARRHPPQESRAEATAALVLWPSHLLGCLNGNGYPLPTPSTLVWAQGSCLRVSLPPARVKAPPTPARPRTHPSQTGPEGESMGTELTPQTQPFSTAKVSRFCPPRKSRHDWIGPPDKYSNLRPIHFYIPENESPLEQKLRELRQETQEWNQQFWADQNLTFHKEKEEFVRSRLKAKGLDLRTASGQKATLNAEEMAEFYKEFLSKNFQKHMYYNRDWYKRNFAITFFMGKVALERIWNKLRPKQKKTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EH39</accession>
    <name>A0A4W2EH39_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EH39"/>
    <dbReference type="STRING" id="30522.A0A4W2EH39"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015751.1">
        <property type="protein sequence ID" value="ENSBIXP00000031696.1"/>
        <property type="gene ID" value="ENSBIXG00000014092.1"/>
    </dbReference>
    <dbReference type="OMA" id="CKPRTLL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042795">
        <property type="entry name" value="Wdr73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46947">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46947:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="369" mass="40456" checksum="189E8D883696DD00" modified="2019-09-18" version="1">MEPAEDWLVESLRLYQDFHAFDLSGATRVLEWIGDKGILVAGYDSLKNEILHLILPLRLSVKENQGLFPERDFKVQHGGFSDRSVCDLKHVPDTRLLVTSGPPGSYLQVWQLAEDSDVIRAVSTIDVHEREEPLWPRVAIFPSVAPGVLHGTRLSSLKVVDLHSQKTMYTSGVSDDDVLSSLQVLDTDTFAFCCSSGRLGLVDTRQRWAPSENLSPGPGSTGRQWCAEAGGRGPGPSIARLGSDGQLCLLDPRDLCQPVSSVQCPVSMPSPEPELLRVTWAPGLDNCLAISGFDGTVQVYDVTSWAGVGRQVEPLFTHRGHIFLDDSGMDPAPLVTTHTWHPYKPRTVLSAASDASLHVWDWVDPCASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIW0</accession>
    <name>A0A4W2EIW0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EIW0"/>
    <dbReference type="STRING" id="30522.A0A4W2EIW0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044379.1">
        <property type="protein sequence ID" value="ENSBIXP00000032846.1"/>
        <property type="gene ID" value="ENSBIXG00000015529.1"/>
    </dbReference>
    <dbReference type="OMA" id="PECVFAE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000080">
        <property type="entry name" value="rRNA processing Fcf1 Family Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="67"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="165" mass="19378" checksum="C39D18C972D75332" modified="2019-09-18" version="1">MGKQKKTWKYATMKRMLSLRDQRLKEKDRLKPKKKEKKDPSALKEREVPQHPSCLFFQYNTQLGPPYHILVDTNFINFSIKAKLDLVQSMMDCLYAKCIPCITDCVMAETEKLGQKYRVALRIAKDPRFERLLCTQKGIYADDCLVQRVTQHKCYIVATVDRDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJC5</accession>
    <name>A0A4W2EJC5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ATP binding cassette subfamily D member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ABCD2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a very long-chain fatty acid(in) + ATP + H2O = a very long-chain fatty acid(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:67081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very long-chain fatty acyl-CoA + H2O = a very long-chain fatty acid + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:67073"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJC5"/>
    <dbReference type="SMR" id="A0A4W2EJC5"/>
    <dbReference type="STRING" id="30522.A0A4W2EJC5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047714.1">
        <property type="protein sequence ID" value="ENSBIXP00000037432.1"/>
        <property type="gene ID" value="ENSBIXG00000003953.1"/>
    </dbReference>
    <dbReference type="OMA" id="DIQAGHF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000800">
        <property type="entry name" value="ATP-binding cassette sub-family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF24">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="111"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="479"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="741" mass="83413" checksum="11DA6483EC274260" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJK2</accession>
    <name>A0A4W2EJK2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 16 member 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SLC16A10</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3,3',5-triiodo-L-thyronine(out) = 3,3',5-triiodo-L-thyronine(in)</text>
            <dbReference type="Rhea" id="RHEA:71811"/>
            <dbReference type="ChEBI" id="CHEBI:533015"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:71812"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:71813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-thyroxine(out) = L-thyroxine(in)</text>
            <dbReference type="Rhea" id="RHEA:71819"/>
            <dbReference type="ChEBI" id="CHEBI:58448"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:71820"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:71821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027407004.1">
        <property type="nucleotide sequence ID" value="XM_027551203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJK2"/>
    <dbReference type="STRING" id="30522.A0A4W2EJK2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019876.1">
        <property type="protein sequence ID" value="ENSBIXP00000033456.1"/>
        <property type="gene ID" value="ENSBIXG00000016265.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011255.1">
        <property type="protein sequence ID" value="ENSBIXP00005002940.1"/>
        <property type="gene ID" value="ENSBIXG00005009197.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898241"/>
    <dbReference type="CTD" id="117247"/>
    <dbReference type="GeneTree" id="ENSGT00940000157966"/>
    <dbReference type="OMA" id="LSYRIWA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015192">
        <property type="term" value="F:L-phenylalanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015196">
        <property type="term" value="F:L-tryptophan transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015349">
        <property type="term" value="F:thyroid hormone transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070460">
        <property type="term" value="P:thyroid-stimulating hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17464">
        <property type="entry name" value="MFS_MCT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000330">
        <property type="entry name" value="monocarboxylate transporter 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000156">
        <property type="entry name" value="monocarboxylate transporter 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF119">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="288"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="515" mass="55251" checksum="A097D615E1B4D688" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMZ2</accession>
    <name>A0A4W2EMZ2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Semaphorin-4C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SEMA4C</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Interacts (via the PDZ-binding motif) with GIPC (via the PDZ domain). Interacts with NCDN. Interacts (via the PDZ-binding motif) with DLG4. Interacts with PLXNB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasmic vesicle</location>
            <location evidence="17">Secretory vesicle</location>
            <location evidence="17">Synaptic vesicle membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Postsynaptic density membrane</location>
            <topology evidence="19">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMZ2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030734.1">
        <property type="protein sequence ID" value="ENSBIXP00000038125.1"/>
        <property type="gene ID" value="ENSBIXG00000021883.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042692">
        <property type="term" value="P:muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11258">
        <property type="entry name" value="Sema_4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000033">
        <property type="entry name" value="Semaphorin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001189">
        <property type="entry name" value="Semaphorin 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000021">
        <property type="entry name" value="Semaphorin 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
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    </evidence>
    <sequence length="818" mass="90829" checksum="BB8D11881B5A8B59" modified="2019-09-18" version="1"> </sequence>
</entry>
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            <title>Haplotype-resolved cattle genomes.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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</entry>
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            <taxon>Pecora</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:host-mediated suppression of symbiont invasion"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of cell migration"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="LRR_TM_domain-containing"/>
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        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="GLYCOPROTEIN V PLATELET"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
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        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
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        <location>
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        <location>
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            <end position="567"/>
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    <feature type="domain" description="LRRCT" evidence="7">
        <location>
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            <end position="475"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
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            <end position="516"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="487"/>
            <end position="515"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="584" mass="64602" checksum="917F58D75519A2A9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERI2</accession>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <cofactor evidence="5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="5">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027536247.1"/>
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        <property type="gene ID" value="ENSBIXG00000004660.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005044901.1">
        <property type="protein sequence ID" value="ENSBIXP00005009863.1"/>
        <property type="gene ID" value="ENSBIXG00005014680.1"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenosylhomocysteinase"/>
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    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase"/>
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    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
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        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
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        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK05476.2-3"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF3">
        <property type="entry name" value="S-ADENOSYLHOMOCYSTEINE HYDROLASE-LIKE PROTEIN 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="8">
        <location>
            <begin position="289"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="320"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="530" mass="58951" checksum="974D23361A245D04" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETN7</accession>
    <name>A0A4W2ETN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ETN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035404.1">
        <property type="protein sequence ID" value="ENSBIXP00000040163.1"/>
        <property type="gene ID" value="ENSBIXG00000004855.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="349" mass="39186" checksum="D087DB1B5523A9A8" modified="2019-09-18" version="1">MPSSHLILCRPLLLLPPIPPSIRVFASESTLCMKWPMYWSFSFSIIPSEEIPGLISFRMDWLDLLAVQGTLKSLLQHHSSKASILQCSAFFTVQPSHPYMTTGKTIDLTRWTFVGKVMSLLFNMLSGWSFPSKDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVHQGCILSPCLFNLDADYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKMESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSEITADGDCSHEIKRRLLLGRKVMTKLDSILKSRDITLPTKVHLVKAMVFPVVMYGCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXF9</accession>
    <name>A0A4W2EXF9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ETS variant transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ETV1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EXF9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038435.1">
        <property type="protein sequence ID" value="ENSBIXP00000041501.1"/>
        <property type="gene ID" value="ENSBIXG00000001949.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000121">
        <property type="entry name" value="ETS translocation variant 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006715">
        <property type="entry name" value="ETS_PEA3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF196">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04621">
        <property type="entry name" value="ETS_PEA3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="10">
        <location>
            <begin position="335"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="128"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="477" mass="55115" checksum="C92E94D507D71555" modified="2019-09-18" version="1">MDGFYDQQVPYMVTNNQRGRNCNEKPTNVRKRKFINRDLAHDSEELFQDLSQLQETWLAEAQVPDNDEQFVPDYQAESLAFHGLPLKIKKEPHSPCSELGSACSQEQPFKFSYGEKCLYNVSAYDQKPQVGMRPSNPPTPSSSPVSPLHHASPNSAHTSKPDRAFPAHLPPSQPIQDSSYPMDHRFRRQLSEPCNSFPPLPTMPREGRPMYQRQMSEPNIPFPPQGFKQEYHDPVYEHNTMVGGAASQSFPPPLMIKQEPRDFAYDSEVPSCHSIYMRQEGFLTHPSRTEGCMFEKGPRQFYDDTCVVPEKFDGDIKQEPGMYREGPTYQRRGSLQLWQFLVALLDDPSNSHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCDPEALFSMAFPDNQRPLLKTDMERHINEEDTVPLSHFDESMAYMPEGGCCNPHPYNEGYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F4C8</accession>
    <name>A0A4W2F4C8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113893471</name>
        <name evidence="16" type="synonym">OR10A7</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399249.1">
        <property type="nucleotide sequence ID" value="XM_027543448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F4C8"/>
    <dbReference type="STRING" id="30522.A0A4W2F4C8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043000.1">
        <property type="protein sequence ID" value="ENSBIXP00000043936.1"/>
        <property type="gene ID" value="ENSBIXG00000028122.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022121.1">
        <property type="protein sequence ID" value="ENSBIXP00005035641.1"/>
        <property type="gene ID" value="ENSBIXG00005016845.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893471"/>
    <dbReference type="GeneTree" id="ENSGT01150000286970"/>
    <dbReference type="OMA" id="MAIGSWM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="316" mass="35769" checksum="E371973B8D9B4B04" modified="2019-09-18" version="1">MICENHTQVTEFILHGFTNKPEMQHSLFVLFLVIYTVTLLGNFLIVTVTIVDPALQTPMYFFLRNLSLLEICFTLVMVPKMLVDLVSPRKSISFVGCGTQMYFFFFFGSSECFLLSMMAYDRFVAICNPLRYSVIMNRSLCLWMAVGSWLSGVPVSMLQTAWLMAHPFCGPSTIDHFFCDGPPVLKLVTEDTTIYEMQALASTLLFIMFPFSLILVSYTRIIKTILMMPSATGRQKAFSTCSSHLIVVSLFYGTASLTYLRPKSKQSPESKKLVSLSYTVITPMLNPIIYSLRNNEVKGAVKRTFTRKVLQKLGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FKW0</accession>
    <name>A0A4W2FKW0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ephrin-A3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">EHK1 ligand</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">EPH-related receptor tyrosine kinase ligand 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">EFNA3</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with EPHA8; activates EPHA8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027387187.1">
        <property type="nucleotide sequence ID" value="XM_027531386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FKW0"/>
    <dbReference type="SMR" id="A0A4W2FKW0"/>
    <dbReference type="STRING" id="30522.A0A4W2FKW0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039745.1">
        <property type="protein sequence ID" value="ENSBIXP00000023941.1"/>
        <property type="gene ID" value="ENSBIXG00000026255.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006682.1">
        <property type="protein sequence ID" value="ENSBIXP00005005273.1"/>
        <property type="gene ID" value="ENSBIXG00005000991.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885770"/>
    <dbReference type="CTD" id="1944"/>
    <dbReference type="GeneTree" id="ENSGT00940000161355"/>
    <dbReference type="OMA" id="LCNFPLC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902993">
        <property type="term" value="P:positive regulation of amyloid precursor protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000030">
        <property type="entry name" value="ephrin-A3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF5">
        <property type="entry name" value="EPHRIN-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611417" description="Ephrin-A3" evidence="16">
        <location>
            <begin position="23"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="17">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00084021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="238" mass="26280" checksum="92343B40161F81AB" modified="2019-09-18" version="1">MAAAPLLLLLLLVPVPLLPLLAQGPGGALGNRHAVYWNSSNQHLRREGYTVQVNVNDYLDIYCPHYNSSGVGPGAGPGPGGGAEQYVLYMVSRDGYRTCNASQGSKRWECNRPHAPHSPIKFSEKFQRYSAFSLGYEFHAGHEYYYISTPTHNLHWKCLRMKVFVCCASTSHSGEKPVPTLPQFTMGPHVKINVLEDFEGENPQVPKLEKSISGTSPKREHLPLAVGIAFFLMTLLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FPB0</accession>
    <name>A0A4W2FPB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZDHHC2</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027385933.1">
        <property type="nucleotide sequence ID" value="XM_027530132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FPB0"/>
    <dbReference type="SMR" id="A0A4W2FPB0"/>
    <dbReference type="STRING" id="30522.A0A4W2FPB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006941.1">
        <property type="protein sequence ID" value="ENSBIXP00000004855.1"/>
        <property type="gene ID" value="ENSBIXG00000011157.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005002755.1">
        <property type="protein sequence ID" value="ENSBIXP00005007259.1"/>
        <property type="gene ID" value="ENSBIXG00005012521.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885021"/>
    <dbReference type="CTD" id="51201"/>
    <dbReference type="GeneTree" id="ENSGT00940000153716"/>
    <dbReference type="OMA" id="CFVVMHI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098837">
        <property type="term" value="C:postsynaptic recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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                <person name="Bickhart D.M."/>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
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        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <sequence length="203" mass="22593" checksum="1A87FD77BEF1659C" modified="2019-09-18" version="1">MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKSEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GDU5</accession>
    <name>A0A4W2GDU5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GDU5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017984.1">
        <property type="protein sequence ID" value="ENSBIXP00000009633.1"/>
        <property type="gene ID" value="ENSBIXG00000015272.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048054.1">
        <property type="protein sequence ID" value="ENSBIXP00005016117.1"/>
        <property type="gene ID" value="ENSBIXG00005019851.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287762"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="44" mass="4920" checksum="32AE405042FE5854" modified="2019-09-18" version="1">MPTSSTIHVLQLLRELLAFVLLSYTVLIGALLLAGWTTYFLVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIY2</accession>
    <name>A0A4W2GIY2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane protein 120B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM120B</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for efficient adipogenesis. Does not show ion channel activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027422650.1">
        <property type="nucleotide sequence ID" value="XM_027566849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GIY2"/>
    <dbReference type="SMR" id="A0A4W2GIY2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017840.1">
        <property type="protein sequence ID" value="ENSBIXP00000009535.1"/>
        <property type="gene ID" value="ENSBIXG00000015186.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028451.1">
        <property type="protein sequence ID" value="ENSBIXP00005016902.1"/>
        <property type="gene ID" value="ENSBIXG00005020423.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907549"/>
    <dbReference type="CTD" id="144404"/>
    <dbReference type="GeneTree" id="ENSGT00390000007848"/>
    <dbReference type="OMA" id="WPNTGPW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="339" mass="40373" checksum="5CBDEE755F272292" modified="2019-09-18" version="1">MSGQLERCEREWHELEGEFQELQETHRIYKQKLEELNALQTSCSSSINKQKTRLKDLKLTLQRYKRHASREEAELVQQMGANIKERQNVFFDMEAYLPKKNGLYLNLVLGNVNVTLLSNQAKFAYKDEYEKFKLYLTIILLLGAVACRFFLHYRVTDEVFNFLLVWYYCTLTIRESILISNGSRIKGWWVSHHYVSTFLSGVMLTWPNGLIYQKFRNQFLAFSIFQSCVQFLQYYYQRGCLYRLRALGERNHLDLTVEGFQSWMWRGLTFLLPFLFCGHFWQLYNAVTLFELSSHEECREWQVFVLALTFLVLFLGNFLTTLKVVHTKLQQNRSKAKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GJ69</accession>
    <name>A0A4W2GJ69_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein arginine N-methyltransferase 3</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PRMT3</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027387200.1">
        <property type="nucleotide sequence ID" value="XM_027531399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GJ69"/>
    <dbReference type="SMR" id="A0A4W2GJ69"/>
    <dbReference type="STRING" id="30522.A0A4W2GJ69"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012687.1">
        <property type="protein sequence ID" value="ENSBIXP00000002042.1"/>
        <property type="gene ID" value="ENSBIXG00000007363.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031418.1">
        <property type="protein sequence ID" value="ENSBIXP00005018853.1"/>
        <property type="gene ID" value="ENSBIXG00005022211.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885775"/>
    <dbReference type="CTD" id="10196"/>
    <dbReference type="GeneTree" id="ENSGT00940000156825"/>
    <dbReference type="OMA" id="YSHFAIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044020">
        <property type="term" value="F:histone H4R3 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900053">
        <property type="term" value="P:negative regulation of retinoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000034">
        <property type="entry name" value="Protein arginine N-methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000005">
        <property type="entry name" value="protein arginine N-methyltransferase 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049482">
        <property type="entry name" value="ANM3-like_C2H2_Zf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049009">
        <property type="entry name" value="ANM3_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21137">
        <property type="entry name" value="ANM3_C2H2_Zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21336">
        <property type="entry name" value="ANM3_zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="527" mass="59222" checksum="6614C8404A7F9E28" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GKE6</accession>
    <name>A0A4W2GKE6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 299</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CFAP299</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027400666.1">
        <property type="nucleotide sequence ID" value="XM_027544865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GKE6"/>
    <dbReference type="STRING" id="30522.A0A4W2GKE6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050103.1">
        <property type="protein sequence ID" value="ENSBIXP00000021698.1"/>
        <property type="gene ID" value="ENSBIXG00000024732.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049326.1">
        <property type="protein sequence ID" value="ENSBIXP00005018581.1"/>
        <property type="gene ID" value="ENSBIXG00005021935.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894438"/>
    <dbReference type="CTD" id="255119"/>
    <dbReference type="GeneTree" id="ENSGT00390000016547"/>
    <dbReference type="OMA" id="FNNYQEY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027887">
        <property type="entry name" value="DUF4464"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14713">
        <property type="entry name" value="DUF4464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="233" mass="26906" checksum="3A34D7A379A7C360" modified="2019-09-18" version="1">MDQDEALTTVDNIVTQFNTYEDFLDSQITTLDLYYLEDEGLARQLVELGYRGTGEVVKREDFEARKAAIEIARLAERTQKKTLTSAGKDLHDNFLKALAVREEDNRSGKVSSVIFIRDKNSHGQEVSGYIDYAHRLKTEDFEVYFSGKKRLLPRPTDMSFYNWDSHIAIWNSTPNYQVIADNPEGLLFKYKRDRKILNVDPKAQPGDNSTRSPILTELYTQVVIFDHVSRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H0A8</accession>
    <name>A0A4W2H0A8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAB6A, member RAS onco family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RAB6A</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027418745.1">
        <property type="nucleotide sequence ID" value="XM_027562944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H0A8"/>
    <dbReference type="SMR" id="A0A4W2H0A8"/>
    <dbReference type="STRING" id="30522.A0A4W2H0A8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021270.1">
        <property type="protein sequence ID" value="ENSBIXP00000034035.1"/>
        <property type="gene ID" value="ENSBIXG00000002751.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052848.1">
        <property type="protein sequence ID" value="ENSBIXP00005025112.1"/>
        <property type="gene ID" value="ENSBIXG00005027661.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905519"/>
    <dbReference type="CTD" id="5870"/>
    <dbReference type="GeneTree" id="ENSGT00940000154769"/>
    <dbReference type="OMA" id="PVSNDGC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000139">
        <property type="entry name" value="ras-related protein Rab-6A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="208" mass="23563" checksum="DD08CC2914669E24" modified="2019-09-18" version="1">MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYV9</accession>
    <name>A0A4W2HYV9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Calcium and integrin-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Calmyrin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CIB3</name>
    </gene>
    <organism evidence="27 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium tip</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HYV9"/>
    <dbReference type="SMR" id="A0A4W2HYV9"/>
    <dbReference type="STRING" id="30522.A0A4W2HYV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040604.1">
        <property type="protein sequence ID" value="ENSBIXP00000024442.1"/>
        <property type="gene ID" value="ENSBIXG00000005623.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021268.1">
        <property type="protein sequence ID" value="ENSBIXP00005035254.1"/>
        <property type="gene ID" value="ENSBIXG00005016387.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160466"/>
    <dbReference type="OMA" id="RDIKAWY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000079">
        <property type="entry name" value="Calcium and integrin-binding family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051433">
        <property type="entry name" value="CIBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791:SF7">
        <property type="entry name" value="CALCIUM AND INTEGRIN-BINDING FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="12" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17" id="KW-0106">Calcium</keyword>
    <keyword evidence="20" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="23" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="21" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0221">Differentiation</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0519">Myristate</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="19" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="EF-hand" evidence="26">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="187" mass="21904" checksum="1AA8E93F56B276DC" modified="2019-09-18" version="1">MGNKQTVFTHEQLEAYQDCTFFTRKEIMRLFYRYQDLAPQLVPLDYTSCPDVKVPYELIGSMPELKDNPFRQRIAQVFSEDGDGHMTLDNFLDMFSVMSEMAPRDLKAYYAFKIYDFNNDDYICAWDLEQTVTKLTRGELSTEEVSLICEKVLDEADGDHDGRLSLEDFQNMILRAPDFLSTFHIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I1K9</accession>
    <name>A0A4W2I1K9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904810</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027417698.1">
        <property type="nucleotide sequence ID" value="XM_027561897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I1K9"/>
    <dbReference type="STRING" id="30522.A0A4W2I1K9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022658.1">
        <property type="protein sequence ID" value="ENSBIXP00000012630.1"/>
        <property type="gene ID" value="ENSBIXG00000002996.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030466.1">
        <property type="protein sequence ID" value="ENSBIXP00005039272.1"/>
        <property type="gene ID" value="ENSBIXG00005021620.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904810"/>
    <dbReference type="GeneTree" id="ENSGT01150000286940"/>
    <dbReference type="OMA" id="VAMAGNC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF194">
        <property type="entry name" value="OLFACTORY RECEPTOR 52N4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
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            <begin position="27"/>
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        <location>
            <begin position="43"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="321" mass="35819" checksum="83CF4BF24FA7FC40" modified="2019-09-18" version="1">MLMLNKTEWVPPSFILNGVPGLEDVHIWISFPFCSMYVVAMAGNCGLLYLIYCEDSLHKPMYYFLAMLSLTDLVMCSSTVPKALFIFWFHLKKIGFDGCLIQMFFVHTFTGMESGVLMLMALDRYVAICYPLRYSTILTNPVIAKVGLSTFLRGMLLIIPFPFLTKRLPYCRGNVIPHTYCDHMSVAKLSCGNIKVNAIYGLIVALLIGGFDILCITASYTMILRAVVSLSSAEARRKAFSTCTAHICAIVFSYSPAFFSFFSHRFGAHTVPPSCHIIVANIYLLLPPTMNPIVYGVKTKQIRDCVLGILSGSKNTKSQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I3B7</accession>
    <name>A0A4W2I3B7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HDAC7</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2I3B7"/>
    <dbReference type="STRING" id="30522.A0A4W2I3B7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030147.1">
        <property type="protein sequence ID" value="ENSBIXP00000037856.1"/>
        <property type="gene ID" value="ENSBIXG00000000251.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049590.1">
        <property type="protein sequence ID" value="ENSBIXP00005039875.1"/>
        <property type="gene ID" value="ENSBIXG00005022454.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159065"/>
    <dbReference type="OMA" id="WPLSWTR"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:protein kinase C binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cell-cell junction assembly"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of mRNA processing"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10008">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF42">
        <property type="entry name" value="HISTONE DEACETYLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="681"/>
            <end position="999"/>
        </location>
    </feature>
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            <begin position="99"/>
            <end position="183"/>
        </location>
    </feature>
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            <end position="369"/>
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            <end position="580"/>
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            <begin position="608"/>
            <end position="652"/>
        </location>
    </feature>
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        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
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            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
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        <location>
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            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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            <begin position="312"/>
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        </location>
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    </feature>
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            <begin position="622"/>
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    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="810"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="675"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="681"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="758"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="983"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1092" mass="117189" checksum="3D065A26E9FE7041" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I640</accession>
    <name>A0A4W2I640_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sal-like protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zinc finger protein Spalt-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SALL2</name>
        <name evidence="20" type="synonym">METTL3</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable transcription factor that plays a role in eye development before, during, and after optic fissure closure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I640"/>
    <dbReference type="STRING" id="30522.A0A4W2I640"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032513.1">
        <property type="protein sequence ID" value="ENSBIXP00000019068.1"/>
        <property type="gene ID" value="ENSBIXG00000004443.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030682.1">
        <property type="protein sequence ID" value="ENSBIXP00005039372.1"/>
        <property type="gene ID" value="ENSBIXG00005003668.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162245"/>
    <dbReference type="OMA" id="STHVWNC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000556">
        <property type="entry name" value="sal-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000574">
        <property type="entry name" value="sal-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000832">
        <property type="entry name" value="sal-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000215">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF15">
        <property type="entry name" value="SAL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="656"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="688"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="907"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="936"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="137"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="711"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="729"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="755"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="799"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1003" mass="104732" checksum="50FC01521C9B222A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I835</accession>
    <name>A0A4W2I835_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Integrator complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">INTS4</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Component of the Integrator complex, composed of core subunits INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, INTS11/CPSF3L, INTS12, INTS13, INTS14 and INTS15. The core complex associates with protein phosphatase 2A subunits PPP2CA and PPP2R1A, to form the Integrator-PP2A (INTAC) complex. INTS4 is part of the RNA endonuclease subcomplex, composed of INTS4, INTS9, INTS11 and inositol hexakisphosphate (InsP6). Interacts with BRAT1; interaction is required for the assembly of the RNA endonuclease subcomplex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Integrator subunit 4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388497.1">
        <property type="nucleotide sequence ID" value="XM_027532696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I835"/>
    <dbReference type="SMR" id="A0A4W2I835"/>
    <dbReference type="STRING" id="30522.A0A4W2I835"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013189.1">
        <property type="protein sequence ID" value="ENSBIXP00000001802.1"/>
        <property type="gene ID" value="ENSBIXG00000008357.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028511.1">
        <property type="protein sequence ID" value="ENSBIXP00005038418.1"/>
        <property type="gene ID" value="ENSBIXG00005020611.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886478"/>
    <dbReference type="CTD" id="92105"/>
    <dbReference type="GeneTree" id="ENSGT00390000010128"/>
    <dbReference type="OMA" id="GNRHPDY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160232">
        <property type="term" value="C:INTAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160240">
        <property type="term" value="P:RNA polymerase II transcription initiation surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000134">
        <property type="entry name" value="Integrator complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000634">
        <property type="entry name" value="integrator complex subunit 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056235">
        <property type="entry name" value="INTS4_8HBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057412">
        <property type="entry name" value="INTS4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938:SF0">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24493">
        <property type="entry name" value="INTS4_8HBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25458">
        <property type="entry name" value="INTS4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="INTS4 8 helical bundle" evidence="12">
        <location>
            <begin position="613"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 4/Protein SIEL C-terminal Ig-like" evidence="13">
        <location>
            <begin position="824"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="964" mass="108401" checksum="D3AE96A7AA8A2433" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IQM3</accession>
    <name>A0A4W2IQM3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonylcarbamoyl-AMP synthase</fullName>
            <ecNumber evidence="5">2.7.7.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">YRDC</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cytoplasmic and mitochondrial threonylcarbamoyl-AMP synthase required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Participates in t(6)A37 formation in cytoplasmic and mitochondrial tRNAs. May regulate the activity of some transporters.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with RSC1A1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027393582.1">
        <property type="nucleotide sequence ID" value="XM_027537781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IQM3"/>
    <dbReference type="SMR" id="A0A4W2IQM3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038543.1">
        <property type="protein sequence ID" value="ENSBIXP00000023107.1"/>
        <property type="gene ID" value="ENSBIXG00000025680.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042097.1">
        <property type="protein sequence ID" value="ENSBIXP00005044628.1"/>
        <property type="gene ID" value="ENSBIXG00005004896.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890115"/>
    <dbReference type="CTD" id="79693"/>
    <dbReference type="GeneTree" id="ENSGT00390000015364"/>
    <dbReference type="OMA" id="YALGCQI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000007">
        <property type="entry name" value="YrdC N6-threonylcarbamoyltransferase domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF10">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="YrdC-like" evidence="17">
        <location>
            <begin position="65"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="276" mass="28893" checksum="797E6BD82305CD10" modified="2019-09-18" version="1">MSPARPCRGLRAVVAASMGLSEGPAGSARSGRLLRSPSPTPGARLLRLPGSGAGRAANPERGGWTEALRAAVAELRAGAVVAVPTDTLYGLACSASCSEALGAVYRVKGRSETKPLAVCLGRVADVYRYCHVRVPEGLLKDLLPGPVTLVMERSEELNKDLNPFTPLVGIRIPDHAFMQDLAQMFGGPLALTSANLSSQSSSLNVEEFQDLWPHLSLIIDGGPIGDGQSPECRLGSTVVDLSVPGKFGIIRPGCALESTSAILQEYGLLPSHGSCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ITR0</accession>
    <name>A0A4W2ITR0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum protein SC65</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leprecan-like protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Prolyl 3-hydroxylase family member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Synaptonemal complex protein SC65</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">P3H4</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PLOD1, P3H3 and PPIB. Identified in a complex with PLOD1 and P3H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the leprecan family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372632.1">
        <property type="nucleotide sequence ID" value="XM_027516831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ITR0"/>
    <dbReference type="STRING" id="30522.A0A4W2ITR0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045045.1">
        <property type="protein sequence ID" value="ENSBIXP00000033736.1"/>
        <property type="gene ID" value="ENSBIXG00000016572.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056599.1">
        <property type="protein sequence ID" value="ENSBIXP00005046765.1"/>
        <property type="gene ID" value="ENSBIXG00005031475.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877080"/>
    <dbReference type="CTD" id="10609"/>
    <dbReference type="GeneTree" id="ENSGT00940000153814"/>
    <dbReference type="OMA" id="EAFCGRN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046849">
        <property type="term" value="P:bone remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032964">
        <property type="term" value="P:collagen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000119">
        <property type="entry name" value="synaptonemal complex protein SC65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052284">
        <property type="entry name" value="Collagen_mod_leprecan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13986:SF4">
        <property type="entry name" value="ENDOPLASMIC RETICULUM PROTEIN SC65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13986">
        <property type="entry name" value="PROTEIN LYSINE HYDROXYLATION COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612210" description="Endoplasmic reticulum protein SC65" evidence="13">
        <location>
            <begin position="19"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Leprecan-like alpha-helical" evidence="14">
        <location>
            <begin position="35"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="387"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057860"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072458"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00082188"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF23557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="442" mass="50727" checksum="2AAE8B387C0B1B70" modified="2019-09-18" version="1">MARTAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLRDSEAFCHANCSGPAPPAAAPPGPALPGPDGGDEWARELRLFGHVLERAACLRRCKRSLPAFQVPYPPRQLLRDFQSRLPYQYLHYAQFKANRLEKAVAAAYTFLQRNPKHELTAKYLSYYRGLLDAADEPLTDLEAQPYEAVFLRAVKLYNSGDFRGSTEDMERALAEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVEKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPEDNVMQQNLVYYRFHRARWGLEEEDFQPREEARLYHNQTAELRELLDFAHMYLQSDDEMELEETEPPMEPEKPPSDAEFEGEGDYEESIYADWWQEPDAKGDEAEAEPEPELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BLI9</accession>
    <name>A0A4W2BLI9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Small G protein signaling modulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SGSM2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RUTBC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372854.1">
        <property type="nucleotide sequence ID" value="XM_027517053.1"/>
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    <dbReference type="SMR" id="A0A4W2BLI9"/>
    <dbReference type="STRING" id="30522.A0A4W2BLI9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015564.1">
        <property type="protein sequence ID" value="ENSBIXP00000000734.1"/>
        <property type="gene ID" value="ENSBIXG00000029440.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005002711.1">
        <property type="protein sequence ID" value="ENSBIXP00005007279.1"/>
        <property type="gene ID" value="ENSBIXG00005012564.1"/>
    </dbReference>
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    <dbReference type="CTD" id="9905"/>
    <dbReference type="GeneTree" id="ENSGT00940000159315"/>
    <dbReference type="OMA" id="CHRSHCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:melanosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="PH_RUTBC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Small G protein signaling modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="small G protein signaling modulator 1"/>
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    </dbReference>
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        <property type="entry name" value="Small G protein signaling modulator 2"/>
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    </dbReference>
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        <property type="entry name" value="Small G protein signaling modulator 2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
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    </dbReference>
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        <property type="entry name" value="Rab-GAP-TBC_dom"/>
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    </dbReference>
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        <property type="entry name" value="Run_dom"/>
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        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RUN_SGSM2"/>
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    <dbReference type="InterPro" id="IPR037745">
        <property type="entry name" value="SGSM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021935">
        <property type="entry name" value="SGSM1/2_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF194">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12068">
        <property type="entry name" value="PH_RBD"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="34"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="611"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="701"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="775"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1049" mass="118197" checksum="B7908A53BA8458DC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BM77</accession>
    <name>A0A4W2BM77_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BM77"/>
    <dbReference type="SMR" id="A0A4W2BM77"/>
    <dbReference type="STRING" id="30522.A0A4W2BM77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014943.1">
        <property type="protein sequence ID" value="ENSBIXP00000001026.1"/>
        <property type="gene ID" value="ENSBIXG00000029067.1"/>
    </dbReference>
    <dbReference type="OMA" id="AQVWTHK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007422">
        <property type="term" value="P:peripheral nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22031">
        <property type="entry name" value="HMG-box_SoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000004">
        <property type="entry name" value="Transcription factor SOX-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022151">
        <property type="entry name" value="Sox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050917">
        <property type="entry name" value="SOX_TF"/>
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            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
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    <sequence length="534" mass="56240" checksum="A49DE02A66E1A7EB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNH1</accession>
    <name>A0A4W2BNH1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">Testis-specific Y-encoded protein 1-like</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
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            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="279" mass="31672" checksum="F3BFDD92BE2BF14F" modified="2019-09-18" version="1">MLCAAGTLQPPVVTPSEEAILFRVEAVQDSQTLVDGDVAGIGREFRLLAEDIMEEVEVVADEEQKQWSSQELEEKTVEEQSQDRPGGPSEHQALDVLKALAVLKALAALQVELSSECEQNHRAYHQRRKHHLAWGSAIIQGIPGFWAKTIMSHPQVSFAPFPSSLSWQVQVRSHLQSHCKLIFSFQDNPYFLNTMIIKEYYLDITGNRACHSTPVHWFWDFEWASPSHSLDTRSLNFLNWLSGHNGPELNTIADLISNDMWDNPLKYYLGEDDSSIRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BPN3</accession>
    <name>A0A4W2BPN3_BOBOX</name>
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        <submittedName>
            <fullName evidence="16">BRCA2 DNA repair associated</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="16 17">
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
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    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Breast cancer susceptibility protein 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BRCA2_OB_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002093">
        <property type="entry name" value="BRCA2_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055077">
        <property type="entry name" value="BRCA2_TR2"/>
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    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="Tower_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22687">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002397">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81872">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCA2 tower domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50138">
        <property type="entry name" value="BRCA2_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="BRCA2" evidence="13">
        <location>
            <begin position="993"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="repeat" description="BRCA2" evidence="13">
        <location>
            <begin position="1206"/>
            <end position="1240"/>
        </location>
    </feature>
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        <location>
            <begin position="1412"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="repeat" description="BRCA2" evidence="13">
        <location>
            <begin position="1513"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="repeat" description="BRCA2" evidence="13">
        <location>
            <begin position="1659"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="repeat" description="BRCA2" evidence="13">
        <location>
            <begin position="1979"/>
            <end position="2013"/>
        </location>
    </feature>
    <feature type="repeat" description="BRCA2" evidence="13">
        <location>
            <begin position="2049"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="domain" description="Tower" evidence="15">
        <location>
            <begin position="2822"/>
            <end position="2863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="505"/>
            <end position="552"/>
        </location>
    </feature>
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    <sequence length="3420" mass="383671" checksum="B0BAE37736507EEF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="796" mass="89268" checksum="901535674157FD8E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRP8</accession>
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            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRP8"/>
    <dbReference type="STRING" id="30522.A0A4W2BRP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011341.1">
        <property type="protein sequence ID" value="ENSBIXP00000002714.1"/>
        <property type="gene ID" value="ENSBIXG00000009175.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049359.1">
        <property type="protein sequence ID" value="ENSBIXP00005039569.1"/>
        <property type="gene ID" value="ENSBIXG00005003708.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159476"/>
    <dbReference type="OMA" id="VRAQKKW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16111">
        <property type="entry name" value="DCX_DCLK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000066">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000057">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000013">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Doublecortin" evidence="13">
        <location>
            <begin position="128"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="551"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="248"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="375"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="469"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="508"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="833" mass="93575" checksum="FF88C2D095BE515C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRW5</accession>
    <name>A0A4W2BRW5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein 4.1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">4.1R</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Band 4.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Erythrocyte membrane protein band 4.1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EPB41L3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2BRW5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011196.1">
        <property type="protein sequence ID" value="ENSBIXP00000002784.1"/>
        <property type="gene ID" value="ENSBIXG00000009256.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17203">
        <property type="entry name" value="FERM_F1_EPB41L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030691">
        <property type="entry name" value="Band4.1-L3_FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008379">
        <property type="entry name" value="Band_4.1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF20">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05902">
        <property type="entry name" value="4_1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BX68"/>
    <dbReference type="SMR" id="A0A4W2BX68"/>
    <dbReference type="STRING" id="30522.A0A4W2BX68"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006838.1">
        <property type="protein sequence ID" value="ENSBIXP00000004911.1"/>
        <property type="gene ID" value="ENSBIXG00000011200.1"/>
    </dbReference>
    <dbReference type="OMA" id="LCPTHMY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044321">
        <property type="term" value="P:response to leptin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07166">
        <property type="entry name" value="NR_DBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000013">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF113">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 1 GROUP D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="20" id="KW-0010">Activator</keyword>
    <keyword evidence="18" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="19 28" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="9 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23 28" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0770">Synapse</keyword>
    <keyword evidence="21 28" id="KW-0804">Transcription</keyword>
    <keyword evidence="16 28" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 28" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 28" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="30">
        <location>
            <begin position="130"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="31">
        <location>
            <begin position="285"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="233"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00039258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00042197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00043097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="613" mass="66785" checksum="F03DCE683424438C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYG9</accession>
    <name>A0A4W2BYG9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bcl-2 homologous antagonist/killer</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Apoptosis regulator BAK</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">BAK1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027380591.1">
        <property type="nucleotide sequence ID" value="XM_027524790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYG9"/>
    <dbReference type="SMR" id="A0A4W2BYG9"/>
    <dbReference type="STRING" id="30522.A0A4W2BYG9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005796.1">
        <property type="protein sequence ID" value="ENSBIXP00000005420.1"/>
        <property type="gene ID" value="ENSBIXG00000007779.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015923.1">
        <property type="protein sequence ID" value="ENSBIXP00005032898.1"/>
        <property type="gene ID" value="ENSBIXG00005001624.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881731"/>
    <dbReference type="CTD" id="578"/>
    <dbReference type="GeneTree" id="ENSGT01130000278292"/>
    <dbReference type="OMA" id="HYIARWI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097145">
        <property type="term" value="C:BAK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902262">
        <property type="term" value="P:apoptotic process involved in blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001783">
        <property type="term" value="P:B cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001782">
        <property type="term" value="P:B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002352">
        <property type="term" value="P:B cell negative selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001974">
        <property type="term" value="P:blood vessel remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060402">
        <property type="term" value="P:calcium ion transport into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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    <accession>A0A4W2C2K2</accession>
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    <feature type="domain" description="Ig-like" evidence="9">
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    <feature type="domain" description="Ig-like" evidence="9">
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    <feature type="domain" description="Ig-like" evidence="9">
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    <sequence length="2867" mass="313771" checksum="8C9B628CA7906846" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C388</accession>
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            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <sequence length="487" mass="55998" checksum="8FDB96B0B8A448DD" modified="2019-09-18" version="1">MNKASGGDGIPVELFQILKDDAVKVLHSMCQQIWKTQQWPQDWKRSVFIPILKKGNAKECSNYRTVALISHASKVMLKILQARLQQYVNCELPDVQAVFRKGRGTRDQTANIHWIIEKAREVQKNIYFCFIDYAKAFDGVDHYKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQTGKGVCQGCILSPCLFNFYAEYIMRNTGLDEAQDGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMESGLITSWEIDRETLETMADFIFGGFKITADGDCSHETKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAEHRRIDAFELRCWRRLLRVPWTARRSNQSILKEISPGCSLEGLMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRGRQRMKWLDGITDSTDMSLGELRELVMDREAWRAAIHGVAKCRTQLSD</sequence>
</entry>
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    <accession>A0A4W2C4J1</accession>
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        <alternativeName>
            <fullName evidence="16">Olf-1/EBF-like 2</fullName>
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        <name evidence="20" type="primary">EBF3</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="12">Transcriptional activator. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.</text>
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        <text evidence="13">Forms either a homodimer or a heterodimer with a related family member.</text>
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        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2 17">Belongs to the COE family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000006469.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005041194.1"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182859"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="IPT_COE"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COE_DBD"/>
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        <property type="entry name" value="COE_DBD_sf"/>
    </dbReference>
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        <property type="entry name" value="COE_HLH"/>
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    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16423">
        <property type="entry name" value="COE1_HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01345">
        <property type="entry name" value="COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="3 17" id="KW-0217">Developmental protein</keyword>
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            <end position="485"/>
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        <source>
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        <source>
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    <sequence length="596" mass="64838" checksum="E5B31573F1B57DF7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <sequence length="340" mass="39239" checksum="6372F07DFC45C94C" modified="2019-09-18" version="1">MEENNRMGKTRNLFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEKLYKKDLHDPDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANIHWIMEKAREFQKNISFCFIDYAKAFDCVNHNKLWKLLKEIGIPDHLTCLLRNLYAGQEATVRTEHGTTEWFQIGKGVHQGCILSPCLFNLHAEYIMRNARLEEAQAGIQIAGRNINNLRYADDTTLMAESEEELKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C813</accession>
    <name>A0A4W2C813_BOBOX</name>
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            <fullName evidence="2">Small EDRK-rich factor 2</fullName>
        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3">Positive regulator of amyloid protein aggregation and proteotoxicity. Induces conformational changes in amyloid proteins, such as HTT, driving them into compact formations preceding the formation of aggregates.</text>
    </comment>
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        <text evidence="1">Belongs to the SERF family.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13596">
        <property type="entry name" value="SMALL EDRK-RICH FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13596:SF2">
        <property type="entry name" value="SMALL EDRK-RICH FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
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    </dbReference>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008982.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="170" mass="18495" checksum="FFE8B7147FC3FE0F" modified="2019-09-18" version="1">MTRGNQRELARQKNMKKQSDSVKGKRRDDGLSAAARKQRVGIQQPYAEFCPIHFHLILCALHTPASCPFCPLVPPSAPSSLPLGTRRSCSRSRKRQTRRRRNPSSFVASCPTLLPFACVPGASPITLAFSPVVLTGPSTDGIPFALSLQRVPFVLPTPQVASLPLGHSWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEX1</accession>
    <name>A0A4W2CEX1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEX1"/>
    <dbReference type="STRING" id="30522.A0A4W2CEX1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020971.1">
        <property type="protein sequence ID" value="ENSBIXP00000011545.1"/>
        <property type="gene ID" value="ENSBIXG00000002619.1"/>
    </dbReference>
    <dbReference type="OMA" id="GVAGRIW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001222">
        <property type="entry name" value="Serine/threonine-protein kinase ppk25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF56">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MARK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="14"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="355"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="517"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="641" mass="70167" checksum="D078BD60D091D36A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGD0</accession>
    <name>A0A4W2CGD0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cell adhesion molecule CEACAM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Carcinoembryonic antigen-related cell adhesion molecule 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CEACAM16</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Required for proper hearing, plays a role in maintaining the integrity of the tectorial membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooligomer; can for homodimers and homotetramers. Interacts with TECTA and TECTB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the immunoglobulin superfamily. CEA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGD0"/>
    <dbReference type="STRING" id="30522.A0A4W2CGD0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021780.1">
        <property type="protein sequence ID" value="ENSBIXP00000012064.1"/>
        <property type="gene ID" value="ENSBIXG00000017194.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029229.1">
        <property type="protein sequence ID" value="ENSBIXP00005038743.1"/>
        <property type="gene ID" value="ENSBIXG00005020909.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263479"/>
    <dbReference type="OMA" id="WCVARSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05740">
        <property type="entry name" value="IgI_hCEACAM_2_4_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05774">
        <property type="entry name" value="IgV_CEACAM_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000947">
        <property type="entry name" value="Carcinoembryonic antigen related cell adhesion molecule 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001283">
        <property type="entry name" value="Carcinoembryonic antigen related cell adhesion molecule 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000244">
        <property type="entry name" value="carcinoembryonic antigen-related cell adhesion molecule 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001282">
        <property type="entry name" value="carcinoembryonic antigen-related cell adhesion molecule 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050831">
        <property type="entry name" value="CEA_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427:SF10">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610484" description="Cell adhesion molecule CEACAM16" evidence="13">
        <location>
            <begin position="21"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="133"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="222"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="424" mass="45978" checksum="93F99FC26D35E38E" modified="2019-09-18" version="1">MVLIRSRWLLLSAAILSAGAEIIITPEPAQPAEGDNVTLAVQGLSGELLAYNWYAGPTLSLSYLVASYIVSTGDETPGPAHTGREAVRPDGGLDIQGALPGHSGTYILQTLNRQFQTEVGYGHMQVYEILAQPVVVANSTALVERRDTLHLTCSSPSPAEVRWFFNGDALPIAVRLGLSPNGRVLTRHGIRREEAGAYQCEVWNPVSVSRSEPVNLTVYFGPERVAILQDSTTRTGCTIKVDFNTSLTLWCVSRSCPEPEYVWTFNGRALKSDQDHLNISSMTATQEGTYTCIAKNPKTLLSGSASVVVKLSAATVIMTIVPVPTRPMEGQDVTLTVQGYPKDLLVYAWYRGPASEPNRLLSQLPSGNWIAGPAHTGREVGFPNCSLLVQKLNLTDAGRYTLKTVTLQGKTETLEVELQVALLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHZ4</accession>
    <name>A0A4W2CHZ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHZ4"/>
    <dbReference type="SMR" id="A0A4W2CHZ4"/>
    <dbReference type="STRING" id="30522.A0A4W2CHZ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022778.1">
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        <property type="gene ID" value="ENSBIXG00000017765.1"/>
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        <property type="component" value="Chromosome 19"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CMRF35-like molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="PANTHER" id="PTHR11860:SF115">
        <property type="entry name" value="IMMUNOGLOBULIN SUBTYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15330">
        <property type="entry name" value="SIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021313647" description="Ig-like domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="299" mass="33203" checksum="21988D5EBFDF97C2" modified="2019-09-18" version="1">MAQPHRAMWLPPALLLLWVPGCLSLSGPHRVRGIVGGSLSVECRYQEKFRNNNKYWCKNSCLLSPNIVATTESKTEVTQGHVSIRDHPANLTFTVTLESLREEDAGTYRCGIDVSYKFDPTFEVEVSVTPAPRPTRPTVTVKTSTITTKVSTVSFTTLATEGTTHSTSSQEEYDPTQTWRLHALLISLALLLLLLGGISLLAWRMVQRWVKAGEKPEPPQSRSQAAEQTELCYANLELQTWPLSGKPVQPTQAEVEYSTVGPPSGDLHYSSIVFDPRSQDSKADRIPSEMPVYSMVKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJ71</accession>
    <name>A0A4W2CJ71_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113904861</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJ71"/>
    <dbReference type="STRING" id="30522.A0A4W2CJ71"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023413.1">
        <property type="protein sequence ID" value="ENSBIXP00000013153.1"/>
        <property type="gene ID" value="ENSBIXG00000018114.1"/>
    </dbReference>
    <dbReference type="OMA" id="FYSTRMC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="310" mass="34218" checksum="17CBE332404C1C16" modified="2019-09-18" version="1">MEAGNHTSVTEFILLGLTEDPTLCGIFFVLFLGIYVIILVGNITIIILIASCSQLHSPMYLFLGHLAFVDIGCSTSVTPMMIIGFLRHRLALLVAGCEAQLCSMVMFGSTDCFLLAVMAYDRCVAICLPLFYSTRMCPRVCVLLVGGSYLGGCVNAWTFGSCLLTLSFCGPNQIDHFFCDFSPLLKLSCSDVSTIEIIPCISSGSILVITVFVISLSYICILNTILKMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSSYSTEQNRVVSVFYTVVIPMLNPLIYSLRNRDVKEALRKAMVRIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK96</accession>
    <name>A0A4W2CK96_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily C member 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Endoplasmic reticulum DNA J domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DNAJC16</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays an important role in regulating the size of autophagosomes during the formation process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027421126.1">
        <property type="nucleotide sequence ID" value="XM_027565325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CK96"/>
    <dbReference type="SMR" id="A0A4W2CK96"/>
    <dbReference type="STRING" id="30522.A0A4W2CK96"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023956.1">
        <property type="protein sequence ID" value="ENSBIXP00000013502.1"/>
        <property type="gene ID" value="ENSBIXG00000018449.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004631.1">
        <property type="protein sequence ID" value="ENSBIXP00005006310.1"/>
        <property type="gene ID" value="ENSBIXG00005001178.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906744"/>
    <dbReference type="CTD" id="23341"/>
    <dbReference type="GeneTree" id="ENSGT00940000155851"/>
    <dbReference type="OMA" id="QPEFAST"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016243">
        <property type="term" value="P:regulation of autophagosome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02963">
        <property type="entry name" value="TRX_DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
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            <end position="93"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00035043"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013502.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="782" mass="90291" checksum="E08DFD66F022C36F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMB0</accession>
    <name>A0A4W2CMB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CCR4-NOT transcription complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CCR4-associated factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CNOT3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2. Interacts with EBF1. Interacts in an RNA-independent manner with BICC1 (via KH domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
        <text evidence="15">NANOS2 promotes its localization to P-body.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CNOT2/3/5 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027514635.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00000013103.1"/>
        <property type="gene ID" value="ENSBIXG00000003121.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033792.1">
        <property type="protein sequence ID" value="ENSBIXP00005020430.1"/>
        <property type="gene ID" value="ENSBIXG00005023531.1"/>
    </dbReference>
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    <dbReference type="CTD" id="4849"/>
    <dbReference type="GeneTree" id="ENSGT00390000014743"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:determination of left/right symmetry"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001829">
        <property type="term" value="P:trophectodermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT transcription complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 15" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 15" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="10 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 15" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="18">
        <location>
            <begin position="613"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="240"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="317"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="331"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="397"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="744" mass="80787" checksum="741616F1088BFBC9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNH6</accession>
    <name>A0A4W2CNH6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/nucleoside cotransporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC28A2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CNH6"/>
    <dbReference type="SMR" id="A0A4W2CNH6"/>
    <dbReference type="STRING" id="30522.A0A4W2CNH6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046656.1">
        <property type="protein sequence ID" value="ENSBIXP00000014679.1"/>
        <property type="gene ID" value="ENSBIXG00000019310.1"/>
    </dbReference>
    <dbReference type="OMA" id="IMLYAMC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005415">
        <property type="term" value="F:nucleoside:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001895">
        <property type="term" value="P:retina homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008276">
        <property type="entry name" value="C_nuclsd_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018270">
        <property type="entry name" value="C_nuclsd_transpt_met_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011657">
        <property type="entry name" value="CNT_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002668">
        <property type="entry name" value="CNT_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00804">
        <property type="entry name" value="nupC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590">
        <property type="entry name" value="SODIUM/NUCLEOSIDE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590:SF11">
        <property type="entry name" value="SODIUM_NUCLEOSIDE COTRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07662">
        <property type="entry name" value="Nucleos_tra2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01773">
        <property type="entry name" value="Nucleos_tra2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter N-terminal" evidence="9">
        <location>
            <begin position="178"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="11">
        <location>
            <begin position="259"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter C-terminal" evidence="10">
        <location>
            <begin position="362"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="659" mass="72310" checksum="054F971160A16077" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNJ9</accession>
    <name>A0A4W2CNJ9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prostate tumor-overexpressed gene 1 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Activates transcription. Required for nuclear translocation of FLOT1. Promotes cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">May interact with CREBBP. Interacts with FLOT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
        <text evidence="6">Translocates from the cytoplasm to the nucleus at the onset of S-phase. Also localizes to lipid rafts.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mediator complex subunit 25 family. PTOV1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CNJ9"/>
    <dbReference type="STRING" id="30522.A0A4W2CNJ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023791.1">
        <property type="protein sequence ID" value="ENSBIXP00000013379.1"/>
        <property type="gene ID" value="ENSBIXG00000018314.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.30:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.30">
        <property type="entry name" value="Mediator complex subunit 25, ACID domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021394">
        <property type="entry name" value="Med25_PTOV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038196">
        <property type="entry name" value="Med25_PTOV_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433:SF3">
        <property type="entry name" value="PROSTATE TUMOR-OVEREXPRESSED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11232">
        <property type="entry name" value="Med25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med25 PTOV" evidence="8">
        <location>
            <begin position="349"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med25 PTOV" evidence="8">
        <location>
            <begin position="513"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="230"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="283"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="674" mass="75050" checksum="37E1A891A5B7FF4A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNZ7</accession>
    <name>A0A4W2CNZ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras-related protein Rab-18</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RAB18</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB18 is required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts. Also required for maintaining endoplasmic reticulum structure. Plays a role in apical endocytosis/recycling. Plays a key role in eye and brain development and neurodegeneration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts (in GTP-bound form) with ZFYVE1. Interacts with ZW10 and this interaction is enhanced in the presence of ZFYVE1. Interacts with BSCL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Golgi apparatus</location>
            <location evidence="13">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027415504.1">
        <property type="nucleotide sequence ID" value="XM_027559703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CNZ7"/>
    <dbReference type="SMR" id="A0A4W2CNZ7"/>
    <dbReference type="STRING" id="30522.A0A4W2CNZ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046104.1">
        <property type="protein sequence ID" value="ENSBIXP00000013562.1"/>
        <property type="gene ID" value="ENSBIXG00000018489.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011935.1">
        <property type="protein sequence ID" value="ENSBIXP00005029061.1"/>
        <property type="gene ID" value="ENSBIXG00005008947.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903501"/>
    <dbReference type="CTD" id="22931"/>
    <dbReference type="GeneTree" id="ENSGT00940000157325"/>
    <dbReference type="OMA" id="RVHKMDV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000430">
        <property type="entry name" value="Probable Ras-related protein Rab-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="234" mass="25969" checksum="321C96A351B66C20" modified="2019-09-18" version="1">MRGSTPRGGEGLRSGRGGRLLAVRSRVRMDEDVLTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIGVDFKVKTISVDGNKAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRDTFVKLDNWLNELETYCTRNDIVNMLVGNKIDKENREVDRNEGLKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESENQNKGVKLTHREEGQGGGACGGYCSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ85</accession>
    <name>A0A4W2CQ85_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQ85"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016176.1">
        <property type="protein sequence ID" value="ENSBIXP00000008481.1"/>
        <property type="gene ID" value="ENSBIXG00000014320.1"/>
    </dbReference>
    <dbReference type="OMA" id="HADGHEQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="196" mass="20836" checksum="08E2B52545E08EEA" modified="2019-09-18" version="1">VGVPQHAGGDIQLWHSLDRAQQECGETQEGPHGPDEQTGALGPPPPPLPPAGHGVHQHTVSVLADGHDQEDADEEVGLDDAVDQAAEERSERPVEFVPDVLCPEGQAQDEHQVRSRQVGQVDFCRVQTPSSHEEDGQHKEVPQQPAEADGEDAGGQHSVQQGPGFPSFVTGCGVRGQGLALVESLELQEGPGRHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSC1</accession>
    <name>A0A4W2CSC1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="SMR" id="A0A4W2CSC1"/>
    <dbReference type="STRING" id="30522.A0A4W2CSC1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027946.1">
        <property type="protein sequence ID" value="ENSBIXP00000016154.1"/>
        <property type="gene ID" value="ENSBIXG00000020424.1"/>
    </dbReference>
    <dbReference type="OMA" id="XSGGRSR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000015">
        <property type="entry name" value="Kinesin family member 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21608">
        <property type="entry name" value="KINESIN-LIKE PROTEIN CG14535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21608:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF26B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="252"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
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        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1912" mass="204404" checksum="B835E85915230079" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTK5</accession>
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        <submittedName>
            <fullName evidence="17">Somatostatin receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SSTR1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="STRING" id="30522.A0A4W2CTK5"/>
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        <property type="protein sequence ID" value="ENSBIXP00000009618.1"/>
        <property type="gene ID" value="ENSBIXG00000015271.1"/>
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    <dbReference type="OMA" id="MVNLAVW"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:neuropeptide binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:somatostatin receptor activity"/>
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        <property type="term" value="P:cellular response to estradiol stimulus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Somatstn_rcpt"/>
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        <property type="entry name" value="Somatstn_rcpt_1"/>
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        <property type="entry name" value="SOMATOSTATIN RECEPTOR TYPE 1"/>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="SOMATOSTATNR"/>
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        <property type="entry name" value="SOMATOSTTN1R"/>
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        <property type="entry name" value="7TM_GPCR_Srsx"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
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            <end position="218"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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        </location>
    </feature>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="146"/>
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        </location>
    </feature>
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        <location>
            <begin position="115"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="525" mass="57598" checksum="7470ED9901268875" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTV3</accession>
    <name>A0A4W2CTV3_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3">Growth hormone-regulated TBC protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GRTP1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
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    <dbReference type="SMR" id="A0A4W2CTV3"/>
    <dbReference type="STRING" id="30522.A0A4W2CTV3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026732.1">
        <property type="protein sequence ID" value="ENSBIXP00000015357.1"/>
        <property type="gene ID" value="ENSBIXG00000019888.1"/>
    </dbReference>
    <dbReference type="OMA" id="DTMIQDS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000029">
        <property type="entry name" value="Growth hormone-regulated TBC protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF10">
        <property type="entry name" value="GROWTH HORMONE-REGULATED TBC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="74"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="343" mass="39090" checksum="9EC22EF67D0F6743" modified="2019-09-18" version="1">MEAAGRAQAARDRVPRIDPYGFERPEDFDYAAYEEFFSTYLVILTRRAIKWSKLLKGGGGVQRSMTVKRYIRKGVPLEHRARVWMGVSGAQARMDRNPGYYQRLLQGERSASLEEAIRTDMNRTFPDNVRFRKDAEPCLQGPLYNVLLAYGHHNHGVGYCQGMNFIAGYLILVTKSEEEAFWLLDALVGRILPADYYSPSMLGLKTDQEVLGELVRTKLPAVAALMHSHGVLWTLVASRWFICLFVDVLPVETVLRVWDCLFSEGSKIIFRVALTLLKHHQASILEATSVPDLCEKFKEITRGRFVTECHSFMQRIFLEPGSLSRASIARLRERCRARLLAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVR3</accession>
    <name>A0A4W2CVR3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dynein axonemal heavy chain 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DNAH6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CVR3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019170.1">
        <property type="protein sequence ID" value="ENSBIXP00000010382.1"/>
        <property type="gene ID" value="ENSBIXG00000015878.1"/>
    </dbReference>
    <dbReference type="OMA" id="VESFDWQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000007">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001143">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000223">
        <property type="entry name" value="Dynein heavy chain 3, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000015">
        <property type="entry name" value="Dynein heavy chain 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000009">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000011">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000006">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF67">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1460"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1741"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2084"/>
            <end position="2237"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2435"/>
            <end position="2593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2898"/>
            <end position="2949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="4154" mass="475687" checksum="81E8B3FFF8E83D11" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWX5</accession>
    <name>A0A4W2CWX5_BOBOX</name>
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        <submittedName>
            <fullName evidence="16">Potassium sodium-activated channel subfamily T member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KCNT2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027420202.1">
        <property type="nucleotide sequence ID" value="XM_027564401.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000010823.1"/>
        <property type="gene ID" value="ENSBIXG00000016253.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Potassium channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000034">
        <property type="entry name" value="Potassium channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium sodium-activated channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY T MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
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    </dbReference>
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    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="64"/>
            <end position="83"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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            <end position="281"/>
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    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="15">
        <location>
            <begin position="299"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="15">
        <location>
            <begin position="718"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1010"/>
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        </location>
    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1135" mass="130656" checksum="D5FEAC35587AC6C1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CX69</accession>
    <name>A0A4W2CX69_BOBOX</name>
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        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC113899608</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="STRING" id="30522.A0A4W2CX69"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030778.1">
        <property type="protein sequence ID" value="ENSBIXP00000018007.1"/>
        <property type="gene ID" value="ENSBIXG00000004225.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0675">Receptor</keyword>
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    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="306" mass="34626" checksum="1B032AD172C2D36C" modified="2019-09-18" version="1">MEESNQSVVSEFVFRGLCDSKDLQKFLFLPFSALYLMTVLGNLFIAFLIITDSHLHSPMYFLLANLSFVDFCLSSVTTPKLITDFLKDNKTISFGGCMSQILFIHFLAGDEMVLLVTMAYDRCVAICKPLHYSRIMDRQKCIWLALISWIIGFVHAISQLAMILDLSFCGPRIVDSFFCDIPEVIKLACMDTHTLRILINADSGVLAVTCFILLLISYTYILVTIHLHSKDGASKALSTCTSHITVVVLFFGPCIFIYFWPLSITWVDKVLSVFYTVITPLLNPAIYTLRNKEIKNAVKRLISHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYL6</accession>
    <name>A0A4W2CYL6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Resistin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistin/FIZZ family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYL6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031644.1">
        <property type="protein sequence ID" value="ENSBIXP00000018541.1"/>
        <property type="gene ID" value="ENSBIXG00000022369.1"/>
    </dbReference>
    <dbReference type="OMA" id="CQCAGID"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16333">
        <property type="entry name" value="RELM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4230:FF:000001">
        <property type="entry name" value="Resistin-like beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4230">
        <property type="entry name" value="Resistin head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009714">
        <property type="entry name" value="RELM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036262">
        <property type="entry name" value="Resistin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21101">
        <property type="entry name" value="RESISTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21101:SF11">
        <property type="entry name" value="RESISTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06954">
        <property type="entry name" value="Resistin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111423">
        <property type="entry name" value="Resistin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="13704" checksum="B6D1C6031E99D3E6" modified="2019-09-18" version="1">MGRGRPSWGFLGFFSCSTCRMKALSFLFIPVLGLLVCGQSLCPIDKAISEKIQEVTTSLVPGAVRIIGLDCRSVTSRGSLVTCPSGFAVTGCTCGSACGSWDVRAETTCHCQCAGMDWTGARCCRLHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYW4</accession>
    <name>A0A4W2CYW4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Keratinocyte differentiation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KDF1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYW4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029342.1">
        <property type="protein sequence ID" value="ENSBIXP00000017073.1"/>
        <property type="gene ID" value="ENSBIXG00000021113.1"/>
    </dbReference>
    <dbReference type="OMA" id="WSKEHNG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003334">
        <property type="term" value="P:keratinocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010482">
        <property type="term" value="P:regulation of epidermal cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028003">
        <property type="entry name" value="KDF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35085">
        <property type="entry name" value="KERATINOCYTE DIFFERENTIATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35085:SF1">
        <property type="entry name" value="KERATINOCYTE DIFFERENTIATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15551">
        <property type="entry name" value="DUF4656"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="398" mass="43415" checksum="F5E3DEBF6675C728" modified="2019-09-18" version="1">MPRPGHPRPSSGPPRLGPWERPAELCLETYDEQARAPPSRRTRRPDPKDPGHHGPESLTFISGSAEQTPEPPACCLLWRPWVWDWCRAAFCFRRCWDCLQRCGACARGCSPCLSSGDSPEGAAEANWVKEHNGVPPSPDHAPPSRRDGQRLKSAMGSSFSYPDVKLKGIPVYPYRSTTSPAPDADSCCKEPLADPPPMRHSLPSTLASSPRGSEEYYSFHESDLDLPEIGSGSMSSREIDVLIFKKLTELFSVHQIDELAKCTSDTVFLEKTSKISDLISSITQDYHLDEQDAEGRLVRGIIRISTRKSRARPQTAEGRSSRAAAPAAAAPDSGHETMVGSGLSQDELTVQISQETTADAIARKLRPYGAPGYPASHDSSFQGTDTDSSGAPLLQVYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZC4</accession>
    <name>A0A4W2CZC4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="25">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">DDX1</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31 32" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="25">The helicase domain is involved in the stimulation of RELA transcriptional activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family. DDX1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="25"/>
    <dbReference type="RefSeq" id="XP_027411835.1">
        <property type="nucleotide sequence ID" value="XM_027556034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZC4"/>
    <dbReference type="SMR" id="A0A4W2CZC4"/>
    <dbReference type="STRING" id="30522.A0A4W2CZC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047903.1">
        <property type="protein sequence ID" value="ENSBIXP00000017309.1"/>
        <property type="gene ID" value="ENSBIXG00000021320.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035040.1">
        <property type="protein sequence ID" value="ENSBIXP00005021277.1"/>
        <property type="gene ID" value="ENSBIXG00005024527.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901547"/>
    <dbReference type="CTD" id="1653"/>
    <dbReference type="GeneTree" id="ENSGT00940000155678"/>
    <dbReference type="OMA" id="KRQQVKF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071920">
        <property type="term" value="C:cleavage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033677">
        <property type="term" value="F:DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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</entry>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="304" mass="32799" checksum="6ADEE0A6164F3D52" modified="2019-09-18" version="1">MSAKSKGTPSSSSPAEGPPAASKTKVKEQIKIIVEDLELVLGDLKDVAKELKEVVDQIDTLTSDLQLEDEMTDSSKTDTLNSSSSGTTASSIEKIKVQANAPLIKPPAHPSAILTVLRKPNPPAPPPRLTPVKCEDSQRVVPTVNPVKTNGTLLRNGGFPGAPTKIPNGDIYCKPSSNLDKAPGQPLMHRPEKDRCPQAGPRERVRFNEKVQYHGYCPDCDTRYHVKNREVHLHSEPVHPPGKLPPQGPPHPLPPHLPPFPLENGGLGISHSNSFPPLRPATVPPPIAPKPQKTILRKSTTTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5A3</accession>
    <name>A0A4W2D5A3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionyl-tRNA formyltransferase, mitochondrial</fullName>
            <ecNumber evidence="3">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-methionyl-tRNA(fMet) + (6R)-10-formyltetrahydrofolate = N-formyl-L-methionyl-tRNA(fMet) + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:24381"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5A3"/>
    <dbReference type="STRING" id="30522.A0A4W2D5A3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049759.1">
        <property type="protein sequence ID" value="ENSBIXP00000021051.1"/>
        <property type="gene ID" value="ENSBIXG00000002614.1"/>
    </dbReference>
    <dbReference type="OMA" id="GASPIHE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12230:FF:000003">
        <property type="entry name" value="methionyl-tRNA formyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="11">
        <location>
            <begin position="190"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="12">
        <location>
            <begin position="316"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="459" mass="51222" checksum="C5B2B25502B67697" modified="2019-09-18" version="1">MQQKSRALLNLQIPFPAPTLMGHASNQASHAPVAERPRGTIFLPGPAPPAWRRLPHRYAPAQGRPPAEEMRVLVRCCCRHLPVGGGAGQRPNPRWRALARLSASPGWEDGQGARVREKPPWRVLFFGNDQFARETLRALHAARENKEEELIEKLEVVTVPSPSPKGLPVKQYAVQSQLPVYEWPDVGSGEYDVGVVASFGRLLSEAFILKFPYGILNVHPSCLPRWRGPAPIIHTILHGDTIAGVTIMQIKPRRFDVGPILKQETVPVPPKSTSKELEAVLSRLGANMLISVLKNLPESLNNGRQQPAEGVTHAPKISAATSCIKWEEQTSEQIFRLYRAVGNIIPLQTLWMDNTIKLLDLVEVDNSILSDSKLTGQAVIPGSVIYHKQSQILLVCCKDDWIGVRSVMLKKTLTATDFYNGYLHPWYQKNSQAQPSQCRFQTLRLPPKKKQKKKIVAMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5J7</accession>
    <name>A0A4W2D5J7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HDAg domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5J7"/>
    <dbReference type="STRING" id="30522.A0A4W2D5J7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048978.1">
        <property type="protein sequence ID" value="ENSBIXP00000019502.1"/>
        <property type="gene ID" value="ENSBIXG00000023112.1"/>
    </dbReference>
    <dbReference type="OMA" id="PLECHYL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037517">
        <property type="entry name" value="HDAG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052828">
        <property type="entry name" value="NELF-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056557">
        <property type="entry name" value="NELF-A_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328:SF4">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A NELF-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23553">
        <property type="entry name" value="NELF-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51838">
        <property type="entry name" value="HDAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDAg" evidence="2">
        <location>
            <begin position="100"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="544" mass="58638" checksum="D193582ED6F8461B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7V3</accession>
    <name>A0A4W2D7V3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7V3"/>
    <dbReference type="STRING" id="30522.A0A4W2D7V3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036915.1">
        <property type="protein sequence ID" value="ENSBIXP00000021987.1"/>
        <property type="gene ID" value="ENSBIXG00000024973.1"/>
    </dbReference>
    <dbReference type="OMA" id="RFSTWQE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF21">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="7">
        <location>
            <begin position="233"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="6">
        <location>
            <begin position="436"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="386"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="825" mass="93760" checksum="766DE4D8D91CE02D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9F6</accession>
    <name>A0A4W2D9F6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase family 38 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9F6"/>
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        <property type="protein sequence ID" value="ENSBIXP00000020810.1"/>
        <property type="gene ID" value="ENSBIXG00000004827.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:lysosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000029">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000005">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF28">
        <property type="entry name" value="EPIDIDYMIS-SPECIFIC ALPHA-MANNOSIDASE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00872">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="8">
        <location>
            <begin position="324"/>
            <end position="404"/>
        </location>
    </feature>
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        <location>
            <begin position="515"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="534"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="964" mass="109874" checksum="1AE4B234D4B98454" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DC92</accession>
    <name>A0A4W2DC92_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DENN domain containing 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DENND4B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSBIXT00000039331.1">
        <property type="protein sequence ID" value="ENSBIXP00000023650.1"/>
        <property type="gene ID" value="ENSBIXG00000005374.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000042">
        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.50:FF:000001">
        <property type="entry name" value="DENN domain containing 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.50">
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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</entry>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50912">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610771" evidence="8">
        <location>
            <begin position="31"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="9">
        <location>
            <begin position="170"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="222"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="268"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="314"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="363"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="410"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="455"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="501"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="548" mass="61778" checksum="A382A6D72B32DC04" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DK07</accession>
    <name>A0A4W2DK07_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DK07"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000051705.1">
        <property type="protein sequence ID" value="ENSBIXP00000024638.1"/>
        <property type="gene ID" value="ENSBIXG00000026766.1"/>
    </dbReference>
    <dbReference type="OMA" id="TSFHEHK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
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        <location>
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            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="289"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="307" mass="34572" checksum="2AABAF193B14BAFB" modified="2019-09-18" version="1">MKTENYTGVREFIFQGFTSFHEHKLTLFVVFLTLYLLTLSGNVIIVTIISIDRHLHTPMYFFLTALSTSETLYTLVIVPRMLSSLVGLSQPISLAGCATQMFFFITLAINNCFLLTAMGYDRYVAICNPLRYMIIMNKRVCAQLVWGACSIGLFVATIQISSVFRLPFCYRKVAHYFCDIRPVMKLSCANTTIHDIINFLISSLVIVVPMVLVFISYVLIISTILKIASAEGRKKAFATCASHLTVVIVHYGCASIAYLKPKSENMRDQDQLISVTYTVSTPLLNPVVYTLRNKEVKDALCRSKFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKM8</accession>
    <name>A0A4W2DKM8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="16">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with FANCD2 and PCNA. Interacts with WDR48. Interacts with ATAD5; the interaction regulates USP1-mediated PCNA deubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKM8"/>
    <dbReference type="SMR" id="A0A4W2DKM8"/>
    <dbReference type="STRING" id="30522.A0A4W2DKM8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041259.1">
        <property type="protein sequence ID" value="ENSBIXP00000024867.1"/>
        <property type="gene ID" value="ENSBIXG00000026997.1"/>
    </dbReference>
    <dbReference type="OMA" id="MNTTCHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02671">
        <property type="entry name" value="Peptidase_C19O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000053">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000077">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033815">
        <property type="entry name" value="USP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF905">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 16" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="18">
        <location>
            <begin position="81"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="234"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="685"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="783" mass="88473" checksum="95CE1D2F09B5787B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DL02</accession>
    <name>A0A4W2DL02_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CORO6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat coronin family.</text>
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        <property type="nucleotide sequence ID" value="XM_027516963.1"/>
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        <property type="gene ID" value="ENSBIXG00000029019.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019076.1">
        <property type="protein sequence ID" value="ENSBIXP00005034297.1"/>
        <property type="gene ID" value="ENSBIXG00005015226.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877141"/>
    <dbReference type="CTD" id="84940"/>
    <dbReference type="GeneTree" id="ENSGT00940000159328"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF23">
        <property type="entry name" value="CORONIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="8">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
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            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
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            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
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        <location>
            <begin position="171"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="471" mass="52772" checksum="0D232A4C5F85AFE1" modified="2019-09-18" version="1">MSRRVVRQSKFRHVFGQAAKADQAYEDIRVSKVTWDSAFCAVNPKFLAIIVEAGGGGAFIVLPLAKTGRVDKNYPLVTGHTAPVLDIDWCPHNDNVIASASDDTTIMVWQIPDYTPVRSITEPIITLEGHSKRVGILSWHPTARNVLLSAGGDNVIIIWNVGTGEVLLSLDDMHPDVIHSVCWNTNGSLLATTCKDKTLRIIDPRKGQVVAERFAAHEGMRPMRAVFTRQGHIFTTGFTRMSQRELGLWDPNNFEEPVALQEMDTSNGVLLPFYDPDSSIVYLCGKGDSSIRYFEITEEPPFVHYLNTFSSKEPQRGMGFMPKRGLDVSKCEIARFYKLHERKCEPIVMTVPRKSDLFQDDLYPDTPGPEPALEADEWLSGQDADPVLISLRDGYVPPKHRELRVTKRNILDVRPPSGPRRSQSASNAPLSQHTLETLLEEIKALREQVQAQEQRITSLENMLCELVDGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMD4</accession>
    <name>A0A4W2DMD4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Integrin subunit alpha L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ITGAL</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMD4"/>
    <dbReference type="STRING" id="30522.A0A4W2DMD4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013192.1">
        <property type="protein sequence ID" value="ENSBIXP00000027481.1"/>
        <property type="gene ID" value="ENSBIXG00000000073.1"/>
    </dbReference>
    <dbReference type="OMA" id="TVCFQLK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000009">
        <property type="entry name" value="Alpha L integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000001">
        <property type="entry name" value="Integrin, alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF84">
        <property type="entry name" value="INTEGRIN ALPHA-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0401">Integrin</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021507499" evidence="17">
        <location>
            <begin position="24"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1086"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="19">
        <location>
            <begin position="153"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="387"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="443"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="504"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="564"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1123"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1140"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1165" mass="128758" checksum="D133012F42E72CCA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNR1</accession>
    <name>A0A4W2DNR1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Carbonyl reductase (NADPH)</fullName>
            <ecNumber evidence="20">1.1.1.184</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CBR3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-nitrosoglutathione + NADPH + H(+) = S-(hydroxysulfenamide)glutathione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 20">
            <text>a secondary alcohol + NADP(+) = a ketone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.184"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>corticosterone + NADPH + H(+) = 20beta-dihydrocorticosterone + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:70219"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16827"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:189050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>cortisol + NADPH + H(+) = 20beta-dihydrocortisol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:70215"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17650"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:139311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70216"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>daunorubicin + NADPH + H(+) = 13-dihydrodaunorubicin + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64677"/>
            <dbReference type="ChEBI" id="CHEBI:75296"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63505"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>menadione + NADPH + H(+) = menadiol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63492"/>
            <dbReference type="ChEBI" id="CHEBI:6746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28869"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>prostaglandin D2 + NADP(+) = 15-oxoprostaglandin D2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57406"/>
            <dbReference type="ChEBI" id="CHEBI:57408"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.196"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:20745"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.197"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:11637"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>prostaglandin E2 + NADP(+) = 15-oxoprostaglandin E2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63476"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:63477"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>prostaglandin F2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:63481"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>prostaglandin F2alpha + NADP(+) = prostaglandin E2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24508"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="1.1.1.189"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:24510"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.184" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNR1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042598.1">
        <property type="protein sequence ID" value="ENSBIXP00000025774.1"/>
        <property type="gene ID" value="ENSBIXG00000027858.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047021">
        <property type="term" value="F:15-hydroxyprostaglandin dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047020">
        <property type="term" value="F:15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050221">
        <property type="term" value="F:prostaglandin E2 9-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000164">
        <property type="entry name" value="Carbonyl reductase [NADPH] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963">
        <property type="entry name" value="CARBONYL REDUCTASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963:SF3">
        <property type="entry name" value="CARBONYL REDUCTASE [NADPH] 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 20" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 20" id="KW-0521">NADP</keyword>
    <keyword evidence="6 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="277" mass="30411" checksum="99794B365A6CA7D9" modified="2019-09-18" version="1">MSSSTRVALVTGANKGLGFAIVRALAGGFQGDVVLTAPDEAQGRAAVQQLQAQGLSPLFHQLDIDDRQSIRALRDFLRKEYGGLDVLVNNAGIAFKTDDPTPFDIQAEMTLKTNFFATRNVCTELLPIVKPHGRVVNVSSSQGSQALENCSEDLQEKFRCETLTEEDLVDLMKKFVEDTKNEVHEREGWPNSAYGVSKLGVTVLSRILARRLEEKRKADRILLNACCPGWVKTDLGGAHASRTVEEGAETPVYLALLPPDATEPHGQLVRDKVVQNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP31</accession>
    <name>A0A4W2DP31_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SPC24</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
            <location evidence="13">Centromere</location>
            <location evidence="13">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the SPC24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DP31"/>
    <dbReference type="STRING" id="30522.A0A4W2DP31"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052734.1">
        <property type="protein sequence ID" value="ENSBIXP00000026180.1"/>
        <property type="gene ID" value="ENSBIXG00000028272.1"/>
    </dbReference>
    <dbReference type="OMA" id="DQLWDFV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11565">
        <property type="entry name" value="RWD_Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.570:FF:000001">
        <property type="entry name" value="SPC24, NDC80 kinetochore complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.570">
        <property type="entry name" value="Ncd80 complex, Spc24 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 13" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 13" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 13" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="79"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="223" mass="25419" checksum="2B882984E1B68540" modified="2019-09-18" version="1">METSLSGMGAGLRGQLNLRGICSKVVMAAFRDMEEVSQGLLSLLGANRAEAQQRRLLGRHEQMVERLLETQDGAEKWLREILTTEKEVAQRLLDAKEQAHQGGVEVRQLEAELQRASEEDAHLKANLLQLTRELEELKEIEADLERQEKEVDEDTTVTIPSAVYVAQLYRRISKIEWDYECEPGMIKGIHHGPSVAQPIHLDSTQLSKKFISDYLWNLVDTEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRE0</accession>
    <name>A0A4W2DRE0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRE0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000308.1">
        <property type="protein sequence ID" value="ENSBIXP00000026766.1"/>
        <property type="gene ID" value="ENSBIXG00000029458.1"/>
    </dbReference>
    <dbReference type="OMA" id="GDLWPHI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF24">
        <property type="entry name" value="PI-PLC X DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="31"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="324" mass="36011" checksum="D106A85BEF09C703" modified="2019-09-18" version="1">MGGQVSSPGSFSGLPYAPKANADWMSALNSQLWDVPLHQLSIPGSHDTMTYCLNKKSPISSKEPRLLHLLCKVLPCVTLPMVLKWSTTQVLSVTEQLDAGVRYLDLRIAHVEDGSERNLHFVHMVYTTALVEDTLTEISEWLQSHPREVVILACRNFEGMTEDLHEYLVGCIKNIFGDMLCPRGEVPTLRQLWSRGQQVILSYEDEASVSRHTELWPGIPYWWGNKVKPQDLVNYLELMKSHGRPGGLFVAGINLTENLEFILVHPAWSLEKLTLSGLPYLCAWVRAQCPGSAAGCTNIIAGDFIGASGFVSDVIGLNQKLLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DS68</accession>
    <name>A0A4W2DS68_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tight junction associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TJAP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DS68"/>
    <dbReference type="SMR" id="A0A4W2DS68"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007298.1">
        <property type="protein sequence ID" value="ENSBIXP00000029388.1"/>
        <property type="gene ID" value="ENSBIXG00000011001.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043441">
        <property type="entry name" value="Tjap1/BEGAIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043470">
        <property type="entry name" value="Tjap1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664:SF3">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15453">
        <property type="entry name" value="Pilt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tight junction-associated protein 1" evidence="6">
        <location>
            <begin position="271"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Tight junction-associated protein 1" evidence="6">
        <location>
            <begin position="469"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
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        <location>
            <begin position="254"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="435"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="42"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
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        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="548" mass="60150" checksum="C9C9AF755D9B1D9B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSC7</accession>
    <name>A0A4W2DSC7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSC7"/>
    <dbReference type="STRING" id="30522.A0A4W2DSC7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050232.1">
        <property type="protein sequence ID" value="ENSBIXP00000021944.1"/>
        <property type="gene ID" value="ENSBIXG00000024933.1"/>
    </dbReference>
    <dbReference type="OMA" id="PSDCACL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
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            <end position="125"/>
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        <location>
            <begin position="46"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="307" mass="33718" checksum="17B0BCD863024525" modified="2019-09-18" version="1">MSIPQNGNLSVMPLQEFVLEGFEGGPQTQALLFALFLALYMVAILGNITMIVVITLDARLHSPMYFFLKNLSFVDLCYSSVIAPKALVNFLSSSKVITFGGCAIQLFFFSLLATTEAFLLAVMAYDHFMAICSPLLYPITMHPSDCACLVLGSYCGGCLNSILQASFTFTLPFCSSNHIDHFHCDVPPLLKLACADTMTNELVMFGLCGFFIVVTILRMHSGGGRHKLFSTCGSHMTAVSLFYGTVFVMYAQPGAVESMEQGKVVSVFYTLVIPMLNPLIYSLRNKDVQEALWRLGQKHTILISKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSF4</accession>
    <name>A0A4W2DSF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSF4"/>
    <dbReference type="STRING" id="30522.A0A4W2DSF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007071.1">
        <property type="protein sequence ID" value="ENSBIXP00000029499.1"/>
        <property type="gene ID" value="ENSBIXG00000011095.1"/>
    </dbReference>
    <dbReference type="OMA" id="SQPSMKF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000002">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF6">
        <property type="entry name" value="TENSIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="9">
        <location>
            <begin position="449"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="334"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="716" mass="75860" checksum="7E71823D91E66A02" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DV50</accession>
    <name>A0A4W2DV50_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LRRC14</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Negatively regulates Toll-like receptor-mediated NF-kappa-B signaling by disrupting IKK core complex formation through interaction with IKBKB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with IKBKB; disrupts IKBKB-IKBKG interaction preventing I-kappa-B-kinase (IKK) core complex formation and leading to a decrease of IKBKB phosphorylation and NF-kappaB activation. Interacts with CHUK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRAME family. LRRC14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416357.1">
        <property type="nucleotide sequence ID" value="XM_027560556.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027416359.1">
        <property type="nucleotide sequence ID" value="XM_027560558.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027416360.1">
        <property type="nucleotide sequence ID" value="XM_027560559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DV50"/>
    <dbReference type="SMR" id="A0A4W2DV50"/>
    <dbReference type="STRING" id="30522.A0A4W2DV50"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004467.1">
        <property type="protein sequence ID" value="ENSBIXP00000030293.1"/>
        <property type="gene ID" value="ENSBIXG00000001143.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008495.1">
        <property type="protein sequence ID" value="ENSBIXP00005004350.1"/>
        <property type="gene ID" value="ENSBIXG00005010312.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903896"/>
    <dbReference type="CTD" id="9684"/>
    <dbReference type="GeneTree" id="ENSGT01030000234531"/>
    <dbReference type="OMA" id="VTECELM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034122">
        <property type="term" value="P:negative regulation of toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000119">
        <property type="entry name" value="Leucine-rich repeat-containing 14 isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050694">
        <property type="entry name" value="LRRC14/PRAME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224">
        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="493" mass="54918" checksum="9DA2857016814323" modified="2019-09-18" version="1">MHTLVFLSTRQVLQCQSAACQALPLLPRELFPLLFKVAFMDKKTVVLRELVHTWPFPLLSFQQLLQECAHCSRALLQERPSTESMQAVILGLTARLHTPETEPGTQPLCRKHTLRVLDMTGLLDDGVEQDPGTMSMWDCTAAVARTCIEQQQGRTAEPGQAPVPVEVRVDLRVNRASYAFLREALRSSVGSPLRLCCRDLRAEDLPMRNTVALLQLLDAGCLRRVDLRFNNLGLRGLSVIIPHVARFQHLASLRLHYVHGDSRQPSVDGEDNFRYFLAQMGRFTCLRELSMGSSLLSGRLDQLLSTLQSPLESLELAFCALLPEDLRFLARSSHAVHLKKLDLSGNDLSGSQLEPFQGLLQAAAATLLHLELTECQLADTQLLATLPVLTRCASLRYLGLYGNPLSVAGLRELLRDSVVQAELRTVVHPFPVDCYEGLPWPPPASVLLEASINEEKFARVEAELHQLLLASGRAHVLWTTDIYGRLAADYFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DVB3</accession>
    <name>A0A4W2DVB3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Structural maintenance of chromosomes flexible hinge domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SMCHD1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer; homodimerizes via its SMC hinge domain. Interacts with LRIF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the SMC family. Highly divergent.</text>
    </comment>
    <dbReference type="SMR" id="A0A4W2DVB3"/>
    <dbReference type="STRING" id="30522.A0A4W2DVB3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010335.1">
        <property type="protein sequence ID" value="ENSBIXP00000028425.1"/>
        <property type="gene ID" value="ENSBIXG00000009602.1"/>
    </dbReference>
    <dbReference type="OMA" id="PIECFNR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001740">
        <property type="term" value="C:Barr body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009048">
        <property type="term" value="P:dosage compensation by inactivation of X chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16937">
        <property type="entry name" value="HATPase_SMCHD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000060">
        <property type="entry name" value="Structural maintenance of chromosomes flexible hinge domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1620:FF:000004">
        <property type="entry name" value="Structural maintenance of chromosomes flexible hinge domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058611">
        <property type="entry name" value="Ig_SMCHD1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058612">
        <property type="entry name" value="Ig_SMCHD1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058613">
        <property type="entry name" value="Ig_SMCHD1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058614">
        <property type="entry name" value="Ig_SMCHD1_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058615">
        <property type="entry name" value="Ig_SMCHD1_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058617">
        <property type="entry name" value="Ig_SMCHD1_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058616">
        <property type="entry name" value="Ig_SMCHD1_8th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038892">
        <property type="entry name" value="SMCHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055109">
        <property type="entry name" value="SMCHD1_S5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640:SF2">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26194">
        <property type="entry name" value="Ig_SMCHD1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26195">
        <property type="entry name" value="Ig_SMCHD1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26196">
        <property type="entry name" value="Ig_SMCHD1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26197">
        <property type="entry name" value="Ig_SMCHD1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26198">
        <property type="entry name" value="Ig_SMCHD1_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26201">
        <property type="entry name" value="Ig_SMCHD1_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26199">
        <property type="entry name" value="Ig_SMCHD1_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22899">
        <property type="entry name" value="SMCHD1_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SMC hinge" evidence="18">
        <location>
            <begin position="1716"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1640"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1997" mass="225295" checksum="4BD09D55674D835B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DW99</accession>
    <name>A0A4W2DW99_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho guanine nucleotide exchange factor 10-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GrinchGEF</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ARHGEF10L</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RHOB and RHOC.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with RHOA, RHOB and RHOC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DW99"/>
    <dbReference type="STRING" id="30522.A0A4W2DW99"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039189.1">
        <property type="protein sequence ID" value="ENSBIXP00000023559.1"/>
        <property type="gene ID" value="ENSBIXG00000001800.1"/>
    </dbReference>
    <dbReference type="OMA" id="CDSHILC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000200">
        <property type="entry name" value="Rho guanine nucleotide exchange factor (GEF) 10-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000003">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 10 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000405">
        <property type="entry name" value="rho guanine nucleotide exchange factor 10-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039919">
        <property type="entry name" value="ARHGEF10/ARHGEF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877:SF16">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="276"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1120" mass="122881" checksum="441E72EB1BD7D96E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0A9</accession>
    <name>A0A4W2E0A9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E0A9"/>
    <dbReference type="STRING" id="30522.A0A4W2E0A9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016978.1">
        <property type="protein sequence ID" value="ENSBIXP00000032223.1"/>
        <property type="gene ID" value="ENSBIXG00000014730.1"/>
    </dbReference>
    <dbReference type="OMA" id="AYSCPIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="585"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="741" mass="83644" checksum="8768F4DD8F3F36A5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3V5</accession>
    <name>A0A4W2E3V5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centrosomal P4.1-associated protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centromere protein J</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Centrosome assembly and centriole elongation protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CENPJ</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms homodimers. Associates with microtubules plus ends; binds to beta-tubulin subunits exposed on microtubule outer surface at its distal tip; also associates with microtubule lattice. Associated with the gamma-tubulin complex. Interacts with the head domain of EPB41. Interacts with LYST. Interacts with CEP152 (via C-terminus). Interacts with STIL. Forms a complex with STIL and SASS6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP10 family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2E3V5"/>
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        <property type="protein sequence ID" value="ENSBIXP00000026327.1"/>
        <property type="gene ID" value="ENSBIXG00000030232.1"/>
    </dbReference>
    <dbReference type="OMA" id="EENSCKH"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061511">
        <property type="term" value="P:centriole elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902117">
        <property type="term" value="P:positive regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Centromere protein J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009852">
        <property type="entry name" value="CENPJ_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047002">
        <property type="entry name" value="Tcp10_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026581">
        <property type="entry name" value="TCP10L/CENPJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058029">
        <property type="entry name" value="Tubulin-bd_CENPJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331:SF23">
        <property type="entry name" value="CENTROMERE PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331">
        <property type="entry name" value="T COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07202">
        <property type="entry name" value="Tcp10_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25779">
        <property type="entry name" value="Tubulin-bind_CPAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENPJ tubulin-binding region" evidence="14">
        <location>
            <begin position="324"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1167"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1236"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1273"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1311"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="612"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="687"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="854"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1105"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="139"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="337"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="753"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="893"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1113"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1155"/>
            <end position="1166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1354" mass="153831" checksum="D8559E8EE3E4B9B4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4J7</accession>
    <name>A0A4W2E4J7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NIPSNAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4J7"/>
    <dbReference type="STRING" id="30522.A0A4W2E4J7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015651.1">
        <property type="protein sequence ID" value="ENSBIXP00000031661.1"/>
        <property type="gene ID" value="ENSBIXG00000008037.1"/>
    </dbReference>
    <dbReference type="OMA" id="QIHEDKH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000003">
        <property type="entry name" value="Protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF14">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="163"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="262" mass="30699" checksum="3BFCBAEC8316A970" modified="2019-09-18" version="1">MASAAHPRSGLPGRASPCRLLPHLRKLTSPNNRPREDGWLKSLFVQKVDPRTDAHSNLLAKKETSSLYKLQFHNVTPERLEAYNKIGQEMLPQIHEDKHYPCTLVGTWNTWYCEQDQAVRLRRYEGGYPALTEVMNKLKENQEFWNSISFWNEPISRSGPNIYELRSCQLRPGTMFQWGNYWARALRFRQEGNEAVGGFFSLIGQLYMVHHLWAYKDLQTREDIRNAAWHKHGWEELVYYTVPLIQERESGIMIPQKTSPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7Q1</accession>
    <name>A0A4W2E7Q1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">FN1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7. Interacts with COMP. Interacts (via type III repeats 9-14) with TNFAIP6 (via CUB domain); this interaction enhances fibronectin fibril assembly. TNFAIP6 may act as a bridging molecule between FN1 and THBS1. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth. Interacts with FST3 and MYOC. Interacts with SVEP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="SMR" id="A0A4W2E7Q1"/>
    <dbReference type="STRING" id="30522.A0A4W2E7Q1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024493.1">
        <property type="protein sequence ID" value="ENSBIXP00000035437.1"/>
        <property type="gene ID" value="ENSBIXG00000018739.1"/>
    </dbReference>
    <dbReference type="OMA" id="GHCITDS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043394">
        <property type="term" value="F:proteoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007044">
        <property type="term" value="P:cell-substrate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00061">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000004">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000006">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000007">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000018">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000027">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000099">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000417">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000579">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000622">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001069">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000017">
        <property type="entry name" value="Fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000020">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000021">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000022">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000227">
        <property type="entry name" value="Fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000275">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000300">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000306">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000317">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000336">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000364">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000382">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000447">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000433">
        <property type="entry name" value="fibronectin isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000083">
        <property type="entry name" value="Fibronectin_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF8">
        <property type="entry name" value="FIBRONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00039">
        <property type="entry name" value="fn1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00058">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01253">
        <property type="entry name" value="FN1_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51091">
        <property type="entry name" value="FN1_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0011">Acute phase</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-0133">Cell shape</keyword>
    <keyword evidence="15 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0558">Oxidation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0765">Sulfation</keyword>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="174"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="219"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="263"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="308"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="353"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="25">
        <location>
            <begin position="479"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="25">
        <location>
            <begin position="539"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="592"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="640"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="683"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="734"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="841"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="937"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1033"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1125"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1213"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1300"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1395"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1484"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1576"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1665"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1759"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1851"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="1939"/>
            <end position="2032"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="23">
        <location>
            <begin position="2033"/>
            <end position="2119"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1207" mass="134282" checksum="394D3B8C08E168C4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEW0</accession>
    <name>A0A4W2EEW0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the P2X receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EEW0"/>
    <dbReference type="STRING" id="30522.A0A4W2EEW0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046302.1">
        <property type="protein sequence ID" value="ENSBIXP00000035517.1"/>
        <property type="gene ID" value="ENSBIXG00000003291.1"/>
    </dbReference>
    <dbReference type="OMA" id="ATMVCDL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003049">
        <property type="entry name" value="P2X6_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053792">
        <property type="entry name" value="P2X_RECEPTOR_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF21">
        <property type="entry name" value="P2X PURINOCEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005713">
        <property type="entry name" value="P2X_purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01313">
        <property type="entry name" value="P2X6RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01212">
        <property type="entry name" value="P2X_RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="242"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="16">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="74"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="375" mass="41584" checksum="C71791BC2EB17CE3" modified="2019-09-18" version="1">LWWALLTKKGYQEQDLDPQVSVITKLKGVSVTRIEELGNRLWDVADFVKPPQGENVFFLVTNFLATPGQVQGRCPEHPSIPLANCWADQDCPEGETGTHSHGIKTGQCVVFNGTHRTCEIRGWCPVESGAVPVKPLLLQAENFTLFIKNTVTFSKFNFSRSNALDTQDPTYFKLCHYDPHSSPACPVFRIGDLVAAAGGVFEDLALLGGAVGVHVRWDCDLDSRGADCRPQYSFQLQERSYNFRTATHWWESSGVEARSLLKLYGIRFDILVTGRAGKFGLIPTLISLGTGAAWLGVITFLCDLLLLYVDGEAHFYWRTKYEEVRCAWGLGAACLGLWPEPVCCHHPQVKAPKRTARPEQTELAPAPPRPAVQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHC9</accession>
    <name>A0A4W2EHC9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat and SOCS box protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EHC9"/>
    <dbReference type="SMR" id="A0A4W2EHC9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047387.1">
        <property type="protein sequence ID" value="ENSBIXP00000037044.1"/>
        <property type="gene ID" value="ENSBIXG00000020501.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000310">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <sequence length="103" mass="11697" checksum="39E14C315DF3F3BF" modified="2019-09-18" version="1">ISVMKPCLMTISILLILVHKTPGGLFRPYYGKSQEESWNPCQLHQGTCRNACRKNELQYLTCLNHEKCCLKFFVKVQSANNVKQESNSNPSLSVTTLSNYSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIC1</accession>
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            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="313" mass="35391" checksum="0634C447C79F0B07" modified="2019-09-18" version="1">MTGRNQTIVSEFLLLGLPIKSEHQNLFYVLFLAMYVTTVLGNLLIIVLICLEPHLHTPMYLFLSNLSFSDLCFSSVTMPKLLQNMHSQDPSISYAGCLTQMYFLLFFGDLESFLLVAMAYDRYVAICFPLRYTTIMSPRLCLFLVVLPWVLTTFHAMLHTLLMARLHFCDDNLIAHFFCDMSALLKLSCSDTRVNELVIFIMGGLILVIPFLLIITSYAQIVSSTLKIPSARGIRKAFSTCGSHLTVVSLFYGTVIGLYLCPSANNSVKETVMATMYTVVTPMLNPFIYSLRNRDMKGALGRVFWRKKAPFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJS8</accession>
    <name>A0A4W2EJS8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2EJS8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020440.1">
        <property type="protein sequence ID" value="ENSBIXP00000011208.1"/>
        <property type="gene ID" value="ENSBIXG00000002568.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000020441.1">
        <property type="protein sequence ID" value="ENSBIXP00000011209.1"/>
        <property type="gene ID" value="ENSBIXG00000002568.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000020448.1">
        <property type="protein sequence ID" value="ENSBIXP00000033700.1"/>
        <property type="gene ID" value="ENSBIXG00000002568.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000020457.1">
        <property type="protein sequence ID" value="ENSBIXP00000033691.1"/>
        <property type="gene ID" value="ENSBIXG00000002568.1"/>
    </dbReference>
    <dbReference type="OMA" id="ICHFLIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000208">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050657">
        <property type="entry name" value="Ankyrin_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039497">
        <property type="entry name" value="CC144C-like_CC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021885">
        <property type="entry name" value="DUF3496"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24147">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24147:SF64">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14915">
        <property type="entry name" value="CCDC144C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12001">
        <property type="entry name" value="DUF3496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="76"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="109"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC144C-like coiled-coil" evidence="8">
        <location>
            <begin position="790"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3496" evidence="7">
        <location>
            <begin position="1229"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="412"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="480"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="683"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="869"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="936"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1116"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1181"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1257"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="480"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1397" mass="159834" checksum="3A022EE0D5F923BA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELF6</accession>
    <name>A0A4W2ELF6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PCMTD2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELF6"/>
    <dbReference type="SMR" id="A0A4W2ELF6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047084.1">
        <property type="protein sequence ID" value="ENSBIXP00000036565.1"/>
        <property type="gene ID" value="ENSBIXG00000020054.1"/>
    </dbReference>
    <dbReference type="OMA" id="SWETKNI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000015">
        <property type="entry name" value="Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF2">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="361" mass="40786" checksum="EEC92608E80B94E7" modified="2019-09-18" version="1">MGGAVSAGEDNDELIDNLKEAQYIRSELVEQAFRAIDRADYYLEEFKENAYKDLAWKHGNIHLSAPCIYSEVMEALDLQPGLSFLNLGSGTGYLSSMVGLILGPFGVNHGVELHSDVTEYAKQKLDFFIRTSDSFDKFDFCEPSFVTGNCLEISPDCSQYDRVYCGAGVQKEHEEYMKSLLKVGGILVMPLEEKLTKITRTGPSAWETKKILAVSFAPLIQPCQSESGKSRLVQLPPLAVRSLQDLARIAIRGTIKKVIHQEAVNKNGNGLKNTPRFKRRRVRRRRLETIVFLDKEVFASRISNPSDDNSSGDLEEERREEEATTPPDAKPEPPVNFLREKVLSLPLPDPLKYYLLYYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMB0</accession>
    <name>A0A4W2EMB0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Unc-50 inner nuclear membrane RNA binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">UNC50</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the cell surface expression of neuronal nicotinic receptors. Binds RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410460.1">
        <property type="nucleotide sequence ID" value="XM_027554659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030429.1">
        <property type="protein sequence ID" value="ENSBIXP00000037988.1"/>
        <property type="gene ID" value="ENSBIXG00000021696.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900950"/>
    <dbReference type="CTD" id="25972"/>
    <dbReference type="OMA" id="YRNFMYR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="260" mass="30427" checksum="9EBC97ABEF7DD63B" modified="2019-09-18" version="1">MLPSTSVNSPAQGNGVLSSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVFIDCVGVGLLISTLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINPDVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSALPFLKNTVILLYPFAPLILLYGLSLALGWNFTHTLCSFYKYRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMQ3</accession>
    <name>A0A4W2EMQ3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113899438</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMQ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048290.1">
        <property type="protein sequence ID" value="ENSBIXP00000038187.1"/>
        <property type="gene ID" value="ENSBIXG00000021963.1"/>
    </dbReference>
    <dbReference type="OMA" id="NRRMCVG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15226">
        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000012">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="315" mass="35510" checksum="6CFA9DF4849FA000" modified="2019-09-18" version="1">MAWNNQSVITEFILQGLSSSWELQMVYFLFFSVVYAATVLGNLLIVLTIVSERRLHSPMYFLLGNLSFIDMSLASFATPKMIADFFREPKVISFDGCITQIFFLHLLGGVEIVLLISMSFDRYVAICKPLRYLTIMNRRVCLGLVTLPWIVGIFHAMSQLAFTVNLPFCGPNEVDSFFCDLPLVIKLACVDTYILGVFMISTSGMIALVCFILLVISYSVILVTVRQRSSGGSSKALSTCSAHFTVVTLFFGPCIFIYVWPFTNFPIDKVLSVFYTIFTPLLNPVIYTLRNKDVKDSMRKFSSRVLKSSKIDHSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERE1</accession>
    <name>A0A4W2ERE1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lamin-B2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LMNB2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Dimer. Lamin dimers then assemble into dimeric head-to-tail polymers. Ultimately, two head-to-tail polymers assemble laterally into a protofilament with a uniformly shaped rod of 3.5 nm in diameter. Interacts with TMEM43.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ERE1"/>
    <dbReference type="STRING" id="30522.A0A4W2ERE1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049177.1">
        <property type="protein sequence ID" value="ENSBIXP00000039469.1"/>
        <property type="gene ID" value="ENSBIXG00000023406.1"/>
    </dbReference>
    <dbReference type="OMA" id="EMTQMRD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090435">
        <property type="term" value="P:protein localization to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1260:FF:000001">
        <property type="entry name" value="Lamin A/C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000076">
        <property type="entry name" value="Lamin B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721">
        <property type="entry name" value="LAMIN DM0-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721:SF2">
        <property type="entry name" value="LAMIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="16">
        <location>
            <begin position="50"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="15">
        <location>
            <begin position="469"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="402"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="588"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="47"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="261"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="595"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="628" mass="70520" checksum="8DB3B57D75D24022" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV72</accession>
    <name>A0A4W2EV72_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113896590</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EV72"/>
    <dbReference type="SMR" id="A0A4W2EV72"/>
    <dbReference type="STRING" id="30522.A0A4W2EV72"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050146.1">
        <property type="protein sequence ID" value="ENSBIXP00000040674.1"/>
        <property type="gene ID" value="ENSBIXG00000024800.1"/>
    </dbReference>
    <dbReference type="OMA" id="VFTPITM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15421">
        <property type="entry name" value="7tmA_OR2T-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="45"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="320" mass="36579" checksum="C9D9A778FEB9CB28" modified="2019-09-18" version="1">MDNSIWVANYTEQLDFILMGLFSQSKYPILLCVVIFVIFLMALSGNIMLILLIYSDAHLHTPMYFFISQLSLMDMIYISVIVPKMLMDQVMGKNKISAPECGMQMFLYLTVGGSEYFLLAAMAYDRYVAICHPLHYSTLMSHKMWLLLVSGCWFLGSVDGFMLTPITMTFPFCRSREIHHFFCEVPAVMKLSCSDTSLYETLMYLCCVLMLLIPVTIISSSYSFILLTIHRMNSAEGRKKAFMTCSSHMTVVILFYGTSTYSYMFPSSYHTSEKDMAVSIFYTILTPVLNPLIYSLRNKDVTGALKNLLNVESAFQRTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWE2</accession>
    <name>A0A4W2EWE2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 2, facilitated glucose transporter member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucose transporter type 1, erythrocyte/brain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SLC2A1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain. In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors. Required for mesendoderm differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027393375.1">
        <property type="nucleotide sequence ID" value="XM_027537574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EWE2"/>
    <dbReference type="SMR" id="A0A4W2EWE2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050675.1">
        <property type="protein sequence ID" value="ENSBIXP00000041362.1"/>
        <property type="gene ID" value="ENSBIXG00000025614.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014032.1">
        <property type="protein sequence ID" value="ENSBIXP00005028413.1"/>
        <property type="gene ID" value="ENSBIXG00005008046.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890035"/>
    <dbReference type="CTD" id="6513"/>
    <dbReference type="GeneTree" id="ENSGT00940000156792"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="101" mass="10808" checksum="33B3CC0F1CBAF09E" modified="2019-09-18" version="1">MHSSSFARSVSPSQGFTSNKLEDLATRSGFIAFFDSYCARRSSHHLLASSLSSSSSDPKRSMSSSLSLLSVAPLSVVSATLAGPYFPKAFFTPDRLAFSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZH0</accession>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="11">Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.</text>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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        <property type="entry name" value="Trypsin_dom"/>
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        <property type="entry name" value="TRYPSIN_HIS"/>
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        <property type="entry name" value="TRYPSIN_SER"/>
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    </dbReference>
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        <property type="entry name" value="CHYMOTRYPSINOGEN B"/>
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    </dbReference>
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        <property type="entry name" value="Trypsin"/>
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    </dbReference>
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        <property type="entry name" value="CHYMOTRYPSIN"/>
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="17">
        <location>
            <begin position="64"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="33391" checksum="434B2D3DBA3C6AFC" modified="2019-09-18" version="1">MTQKPSQKSNIGIQLPINGLPVENNTRRQPAQDSCGVPFVDPFLIEGFERNTNILPAEVGEPRVVGGRAAAVMSWPWLVSLQHQGHHYCGGALIGRRWVLTAAHCNFSTVTDYLVIGRSYLGNIRNSDLIPVKAVYIHPSFTQFPPNDDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSKCLTAGWGITEPHQDEFPKTVQQAKVPLISSISCRSYWGLEIKNTNICGGASGSSSCMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAYTDWITSITGGEAGSLGDQPGGTVITSGKGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZZ1</accession>
    <name>A0A4W2EZZ1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZZ1"/>
    <dbReference type="STRING" id="30522.A0A4W2EZZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040156.1">
        <property type="protein sequence ID" value="ENSBIXP00000042386.1"/>
        <property type="gene ID" value="ENSBIXG00000026459.1"/>
    </dbReference>
    <dbReference type="OMA" id="RIKCSRI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000006">
        <property type="entry name" value="aryl hydrocarbon receptor nuclear translocator-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000010">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator-like, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050933">
        <property type="entry name" value="Circadian_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23042">
        <property type="entry name" value="CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="107"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="178"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="378"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="633" mass="70842" checksum="AF80A509D862ADAE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0P8</accession>
    <name>A0A4W2F0P8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0P8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040577.1">
        <property type="protein sequence ID" value="ENSBIXP00000042618.1"/>
        <property type="gene ID" value="ENSBIXG00000005617.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="198"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="379"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="503" mass="57931" checksum="9512051646E2214E" modified="2019-09-18" version="1">MEVCDIVQETGMKTIPKKKKCKKAKWLSEEALQIALKRREVKSKGEKERYKHLNAEFQRIARRDKKALLSDQCKEIEENNRMGKTRDLFKKIRDTKGTFHTKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHNPDNHDGVITHLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKKSVFISIPKKGSAKECSNYCTIALISHASKVMLKILQARLQQYMNRELPDIQAGFRKGRGTRDQITNICWIMGKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCISSPCLFNFYAEYIMRNAGLEEAQAGMKIARRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLSIQKTKIMASGPITSWQIDGETVETVSDFIFGGSKITADSDCSHEIKRHLLLERKVMTNLDSIFKSRDITLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2R6</accession>
    <name>A0A4W2F2R6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibroleukin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Fibrinogen-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FGL2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Tearle R."/>
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                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="180" mass="18617" checksum="8C3189F866241404" modified="2019-09-18" version="1">MRTWDAPGNPHRPASTRSSKNKASGGGKGKARPARGRAHAVLRARAPPASPTDQPEKGARLPAPGAPCQVPPAGRAPHLACTPPAILRHPAIQRDALRTDGADMGRAIVARLGLGLLLLALLLPTQTYSNQTTVVTPSNSSQTTSPAPHPANATTKASDGTLQSTASLFVISVSLLHLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FKC8</accession>
    <name>A0A4W2FKC8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Lysyl oxidase homolog</fullName>
            <ecNumber evidence="21">1.4.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOXL3</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 21">
            <text>L-lysyl-[protein] + O2 + H2O = (S)-2-amino-6-oxohexanoyl-[protein] + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24544"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="EC" id="1.4.3.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(6)-acetyl-L-lysyl-[protein] + O2 + H2O = acetamide + (S)-2-amino-6-oxohexanoyl-[protein] + H2O2</text>
            <dbReference type="Rhea" id="RHEA:51648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:27856"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>lysine tyrosylquinone residue</name>
            <dbReference type="ChEBI" id="CHEBI:20489"/>
        </cofactor>
        <text evidence="21">Contains 1 lysine tyrosylquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 21">Secreted</location>
            <location evidence="3 21">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="21">The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the lysyl oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.13" evidence="21"/>
    <dbReference type="RefSeq" id="XP_027410601.1">
        <property type="nucleotide sequence ID" value="XM_027554800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FKC8"/>
    <dbReference type="STRING" id="30522.A0A4W2FKC8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046586.1">
        <property type="protein sequence ID" value="ENSBIXP00000035902.1"/>
        <property type="gene ID" value="ENSBIXG00000019241.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005467.1">
        <property type="protein sequence ID" value="ENSBIXP00005005892.1"/>
        <property type="gene ID" value="ENSBIXG00005011540.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901005"/>
    <dbReference type="CTD" id="84695"/>
    <dbReference type="GeneTree" id="ENSGT00940000158157"/>
    <dbReference type="OMA" id="HWGLICG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905590">
        <property type="term" value="P:fibronectin fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030324">
        <property type="term" value="P:lung development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000329">
        <property type="term" value="P:negative regulation of T-helper 17 cell lineage commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060021">
        <property type="term" value="P:roof of mouth development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061053">
        <property type="term" value="P:somite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021510">
        <property type="term" value="P:spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000008">
        <property type="entry name" value="Lysyl oxidase homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000022">
        <property type="entry name" value="lysyl oxidase homolog 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050912">
        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019828">
        <property type="entry name" value="Lysyl_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF2">
        <property type="entry name" value="LYSYL OXIDASE HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
        <property type="entry name" value="Lysyl_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00074">
        <property type="entry name" value="LYSYLOXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00926">
        <property type="entry name" value="LYSYL_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 21" id="KW-0886">LTQ</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 21" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="12 21" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611377" description="Lysyl oxidase homolog" evidence="23">
        <location>
            <begin position="26"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="24">
        <location>
            <begin position="44"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="24">
        <location>
            <begin position="169"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="24">
        <location>
            <begin position="306"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="24">
        <location>
            <begin position="416"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="70"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="83"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="331"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="344"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU367046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="752" mass="82977" checksum="BB4484D60455E33B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G1U8</accession>
    <name>A0A4W2G1U8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyl-protein thioesterase ABHD10, mitochondrial</fullName>
            <ecNumber evidence="7">3.1.1.93</ecNumber>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-protein thioesterase ABHD10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Alpha/beta hydrolase domain-containing protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Mycophenolic acid acyl-glucuronide esterase, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ABHD10</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as an acyl-protein thioesterase that hydrolyzes fatty acids from acylated residues in proteins. Regulates the mitochondrial S-depalmitoylation of the nucleophilic active site residue of peroxiredoxin-5/PRDX5, a key antioxidant protein, therefore modulating mitochondrial antioxidant ability. Also catalyzes the deglucuronidation of mycophenolic acid acyl-glucuronide, an active metabolite of the immunosuppressant drug mycophenolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>mycophenolic acid O-acyl-beta-D-glucuronide + H2O = mycophenolate + D-glucuronate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:62932"/>
            <dbReference type="ChEBI" id="CHEBI:66982"/>
            <dbReference type="EC" id="3.1.1.93"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:34180"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.93" evidence="7"/>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2G1U8"/>
    <dbReference type="STRING" id="30522.A0A4W2G1U8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030215.1">
        <property type="protein sequence ID" value="ENSBIXP00000017609.1"/>
        <property type="gene ID" value="ENSBIXG00000021602.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019389.1">
        <property type="protein sequence ID" value="ENSBIXP00005010809.1"/>
        <property type="gene ID" value="ENSBIXG00005015384.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017765"/>
    <dbReference type="OMA" id="TISRWLE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102390">
        <property type="term" value="F:mycophenolic acid acyl-glucuronide esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000164">
        <property type="entry name" value="Mycophenolic acid acyl-glucuronide esterase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052382">
        <property type="entry name" value="ABHD10_acyl-thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16138">
        <property type="entry name" value="MYCOPHENOLIC ACID ACYL-GLUCURONIDE ESTERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16138:SF7">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE ABHD10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="15">
        <location>
            <begin position="102"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="330" mass="36403" checksum="E8D943744CCFC7A7" modified="2019-09-18" version="1">MRTLVSGRSAHLSRRAQGAGAAGKMAGVGLAAVPAWVPCRRWGLAAVTFGFHHGLSTLLARKTERAPQWLRACRHKTSISFLSRPDLPNLAYKRLKGKSPGIIFIPGYISNMNGTKALAIEEFCKSLGHAYIRFDYSGVGNSDGNLEECTVGKWRKDVLSIIDDLAEGPQILVGSSLGGWLMFHAAIARPQKVVALVGVATAVDGLVTQFNQLPIETKKEIEMKGVWPMPSKYSEEGVYRIQYSVIKEAEHHCLLHSPIPVKCPVRLLHGMKDDIVPWHTSVQVADRVVSTDVDVILRKNSDHRMKEKADIQLLVYTIDDLIDKLSTIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G8X7</accession>
    <name>A0A4W2G8X7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit F</fullName>
            <shortName evidence="5">eIF3f</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">eIF-3-epsilon</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">eIF3 p47</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">EIF3F</name>
        <name evidence="5" type="synonym">EIF3S5</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylated. Phosphorylation is enhanced upon serum stimulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit F family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027418997.1">
        <property type="nucleotide sequence ID" value="XM_027563196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G8X7"/>
    <dbReference type="SMR" id="A0A4W2G8X7"/>
    <dbReference type="STRING" id="30522.A0A4W2G8X7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023477.1">
        <property type="protein sequence ID" value="ENSBIXP00000035015.1"/>
        <property type="gene ID" value="ENSBIXG00000018161.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024690.1">
        <property type="protein sequence ID" value="ENSBIXP00005014372.1"/>
        <property type="gene ID" value="ENSBIXG00005018389.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905639"/>
    <dbReference type="CTD" id="8665"/>
    <dbReference type="GeneTree" id="ENSGT00950000183073"/>
    <dbReference type="OMA" id="EYFVHFH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075522">
        <property type="term" value="P:IRES-dependent viral translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08064">
        <property type="entry name" value="MPN_eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000014">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03005">
        <property type="entry name" value="eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027531">
        <property type="entry name" value="eIF3f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF6">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="125"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="5">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00059951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="390" mass="40895" checksum="8B8C7A4446B52329" modified="2019-09-18" version="1">MALLMFSNSLPSAPRSFSLDKMATPAVPASAPPATPSEAPAAASSPASAPVPAPAPASASAPAPSPAPAAAPSPAPASSSDPAAAAATTAAPGQTPASAPAPAQTPAQSLSGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYTYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPEDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GFM6</accession>
    <name>A0A4W2GFM6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Ia antigen-associated invariant chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">CD74</name>
    </gene>
    <organism evidence="32 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with the mature form of CTSL; the complex survive in neutral pH environment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="28">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401758.1">
        <property type="nucleotide sequence ID" value="XM_027545957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GFM6"/>
    <dbReference type="SMR" id="A0A4W2GFM6"/>
    <dbReference type="STRING" id="30522.A0A4W2GFM6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041194.1">
        <property type="protein sequence ID" value="ENSBIXP00000024827.1"/>
        <property type="gene ID" value="ENSBIXG00000007876.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048585.1">
        <property type="protein sequence ID" value="ENSBIXP00005017160.1"/>
        <property type="gene ID" value="ENSBIXG00005020644.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895160"/>
    <dbReference type="CTD" id="972"/>
    <dbReference type="GeneTree" id="ENSGT00390000008961"/>
    <dbReference type="OMA" id="HHNCSEP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035692">
        <property type="term" value="C:macrophage migration inhibitory factor receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035693">
        <property type="term" value="C:NOS2-CD74 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042609">
        <property type="term" value="F:CD4 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035718">
        <property type="term" value="F:macrophage migration inhibitory factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042658">
        <property type="term" value="F:MHC class II protein binding, via antigen binding groove"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050998">
        <property type="term" value="F:nitric-oxide synthase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019886">
        <property type="term" value="P:antigen processing and presentation of exogenous peptide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035691">
        <property type="term" value="P:macrophage migration inhibitory factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043518">
        <property type="term" value="P:negative regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902166">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002906">
        <property type="term" value="P:negative regulation of mature B cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002792">
        <property type="term" value="P:negative regulation of peptide secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045581">
        <property type="term" value="P:negative regulation of T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045060">
        <property type="term" value="P:negative thymic T cell selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000343">
        <property type="term" value="P:positive regulation of chemokine (C-X-C motif) ligand 2 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002606">
        <property type="term" value="P:positive regulation of dendritic cell antigen processing and presentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048146">
        <property type="term" value="P:positive regulation of fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032757">
        <property type="term" value="P:positive regulation of interleukin-8 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060907">
        <property type="term" value="P:positive regulation of macrophage cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000448">
        <property type="term" value="P:positive regulation of macrophage migration inhibitory factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045657">
        <property type="term" value="P:positive regulation of monocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090023">
        <property type="term" value="P:positive regulation of neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031394">
        <property type="term" value="P:positive regulation of prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045582">
        <property type="term" value="P:positive regulation of T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002830">
        <property type="term" value="P:positive regulation of type 2 immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046598">
        <property type="term" value="P:positive regulation of viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045059">
        <property type="term" value="P:positive thymic T cell selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070206">
        <property type="term" value="P:protein trimerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034341">
        <property type="term" value="P:response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002286">
        <property type="term" value="P:T cell activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.870.10:FF:000001">
        <property type="entry name" value="HLA class II histocompatibility antigen gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000001">
        <property type="entry name" value="Testican-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MHC class II-associated invariant chain, trimerisation domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043530">
        <property type="entry name" value="CD74_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052001">
        <property type="entry name" value="MHC-II_Gamma/Thyroglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015386">
        <property type="entry name" value="MHC_II-assoc_invar/CLIP_MHC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022339">
        <property type="entry name" value="MHC_II-assoc_invar_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011988">
        <property type="entry name" value="MHC_II-assoc_invariant_trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036613">
        <property type="entry name" value="MHCII_invariant_trimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14093">
        <property type="entry name" value="HLA CLASS II GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14093:SF17">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09307">
        <property type="entry name" value="MHC2-interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08831">
        <property type="entry name" value="MHCassoc_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001992">
        <property type="entry name" value="CD74_antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01990">
        <property type="entry name" value="CD74ANTIGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48305">
        <property type="entry name" value="Class II MHC-associated invariant chain ectoplasmic trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
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            <begin position="192"/>
            <end position="253"/>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="27 28">
        <location>
            <begin position="225"/>
            <end position="232"/>
        </location>
    </feature>
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        <location>
            <begin position="234"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
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    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    </evidence>
    <sequence length="268" mass="30326" checksum="4153954720D1EE03" modified="2019-09-18" version="1">MEDQRDLISNHEQLPMLGQRPGAQESKCSRGALYTGFSVLVALLLAGQATTAYFLYQQQGRLDKLTVTSQNLQLENLRMKLPKPAKPMSQMRMATPMLMRALPMAGPEPMKNATKYGNMTQDHVMHLLLKADPLKVYPQLKGSLPENLKHLKDSMDGLDWKLFESWLHQWLLFEMSKNSLEEKPFEGPPKVLTQCQEEVSRIPAIHPGVFKPNCDENGNYMPLQCYGSIGYCWCVFPNGTEVPHTRSRGHRNCSDPMEMEYPSSGLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIP6</accession>
    <name>A0A4W2GIP6_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Protein FAM209A-like</fullName>
        </submittedName>
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        <name evidence="4" type="synonym">LOC113889021</name>
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    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GTT8</accession>
    <name>A0A4W2GTT8_BOBOX</name>
    <protein>
        <recommendedName>
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        </recommendedName>
    </protein>
    <gene>
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    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Component of the CMG helicase complex, a hexameric ring of related MCM2-7 subunits stabilized by CDC45 and the tetrameric GINS complex. Interacts with ANKRD17. Interacts with MCMBP. Interacts with TONSL; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2GTT8"/>
    <dbReference type="STRING" id="30522.A0A4W2GTT8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051863.1">
        <property type="protein sequence ID" value="ENSBIXP00000024885.1"/>
        <property type="gene ID" value="ENSBIXG00000027020.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035696.1">
        <property type="protein sequence ID" value="ENSBIXP00005021704.1"/>
        <property type="gene ID" value="ENSBIXG00005024670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286966"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000005">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000006">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000241">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 19" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="8 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="21">
        <location>
            <begin position="331"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="713"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="780"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="785"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="892" mass="99183" checksum="C0E9A8F2130ADB40" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GXE6</accession>
    <name>A0A4W2GXE6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S23</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the 40S small ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000021544.1"/>
    </dbReference>
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        <property type="gene ID" value="ENSBIXG00005026182.1"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
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    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_S12"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="124" mass="13951" checksum="C4C8AB3C468273A7" modified="2019-09-18" version="1">MGKCHGLCTARKLRSHQRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKRPNSAIRKCVRVQRIKNGKKITSPRVRHLFAVLHSTDGPASRAHQNQELRGRYSMVRQPMVLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HKI8</accession>
    <name>A0A4W2HKI8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Centrosomal protein 83</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CEP83</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027398949.1">
        <property type="nucleotide sequence ID" value="XM_027543148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HKI8"/>
    <dbReference type="STRING" id="30522.A0A4W2HKI8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033335.1">
        <property type="protein sequence ID" value="ENSBIXP00000019648.1"/>
        <property type="gene ID" value="ENSBIXG00000023213.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003569.1">
        <property type="protein sequence ID" value="ENSBIXP00005031830.1"/>
        <property type="gene ID" value="ENSBIXG00005012266.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893266"/>
    <dbReference type="CTD" id="51134"/>
    <dbReference type="GeneTree" id="ENSGT00940000154003"/>
    <dbReference type="OMA" id="DAYKRKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051660">
        <property type="term" value="P:establishment of centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071539">
        <property type="term" value="P:protein localization to centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052116">
        <property type="entry name" value="Centro_Cilium_Assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 83 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170">
        <property type="entry name" value="NY-REN-58 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="108"/>
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        <location>
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            <end position="631"/>
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        <location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="700" mass="82597" checksum="4FFD346072068B26" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HN09</accession>
    <name>A0A4W2HN09_BOBOX</name>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="155" mass="17737" checksum="8D90EDD151720A30" modified="2019-09-18" version="1">MMSTEQMQPLELSEDRLDKLDPRCSHLDDLSDQFIKDCDLKKKPIKGKNVQVTLNVESDQKKPRRKDTPALHVPPFIPDVLSEHLITRYDVQDIQPKGKMSPVLHNTDLEQKKPRRKDTPALHVSPFAAGVTLLRDERPKVIVEDDEKDGDKIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HTB7</accession>
    <name>A0A4W2HTB7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PDK4</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027395919.1">
        <property type="nucleotide sequence ID" value="XM_027540118.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2HTB7"/>
    <dbReference type="STRING" id="30522.A0A4W2HTB7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050585.1">
        <property type="protein sequence ID" value="ENSBIXP00000022644.1"/>
        <property type="gene ID" value="ENSBIXG00000025436.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020294.1">
        <property type="protein sequence ID" value="ENSBIXP00005034817.1"/>
        <property type="gene ID" value="ENSBIXG00005015845.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891714"/>
    <dbReference type="CTD" id="5166"/>
    <dbReference type="GeneTree" id="ENSGT01030000234646"/>
    <dbReference type="OMA" id="HQENCPS"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071398">
        <property type="term" value="P:cellular response to fatty acid"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000811">
        <property type="term" value="P:negative regulation of anoikis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045124">
        <property type="term" value="P:regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046320">
        <property type="term" value="P:regulation of fatty acid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010510">
        <property type="term" value="P:regulation of pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.20:FF:000001">
        <property type="entry name" value="[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000007">
        <property type="entry name" value="Mitochondrial pyruvate dehydrogenase kinase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF22">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE ISOZYME 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="240"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="407" mass="46159" checksum="F06A2BC8449E19B2" modified="2019-09-18" version="1">MKAARFVMRSAGTLIPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIYILPDRLVNTSSVQLVKSWYMQSLMELVEFHEKSPEDQKALSDFVDTLIKVRNRHHDVIPTMAQGILEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFGDLQTGNPSLIGSIDPNCDVAAVVQDAFECSKMLCDQYYLTSPELNLTQVNVKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQQNWPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRVIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLPGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQTSSEAGDWCIPSKEPKNLAKEKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I346</accession>
    <name>A0A4W2I346_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Poly(rC) binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PCBP3</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I346"/>
    <dbReference type="SMR" id="A0A4W2I346"/>
    <dbReference type="STRING" id="30522.A0A4W2I346"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042318.1">
        <property type="protein sequence ID" value="ENSBIXP00000043524.1"/>
        <property type="gene ID" value="ENSBIXG00000027656.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025266.1">
        <property type="protein sequence ID" value="ENSBIXP00005036972.1"/>
        <property type="gene ID" value="ENSBIXG00005018711.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162185"/>
    <dbReference type="OMA" id="MQVVPYS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990829">
        <property type="term" value="F:C-rich single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22516">
        <property type="entry name" value="KH-I_PCBP3_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22519">
        <property type="entry name" value="KH-I_PCBP3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22522">
        <property type="entry name" value="KH-I_PCBP3_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="96"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="282"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005036972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="361" mass="38117" checksum="84AB486FE2241EFB" modified="2019-09-18" version="1">MESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKKMREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLESPPKGATIPYRPKPASTPVIFAGGQVRADPLAASTANLSLLLQPPPLPAYTIQGQYAIPHPDLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IGM1</accession>
    <name>A0A4W2IGM1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit D</fullName>
            <shortName evidence="5">eIF3d</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">eIF-3-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">EIF3D</name>
        <name evidence="5" type="synonym">EIF3S7</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylated upon DNA damage, probably by ATM or ATR.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit D family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397559.1">
        <property type="nucleotide sequence ID" value="XM_027541758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IGM1"/>
    <dbReference type="SMR" id="A0A4W2IGM1"/>
    <dbReference type="STRING" id="30522.A0A4W2IGM1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051770.1">
        <property type="protein sequence ID" value="ENSBIXP00000024741.1"/>
        <property type="gene ID" value="ENSBIXG00000026881.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037495.1">
        <property type="protein sequence ID" value="ENSBIXP00005042367.1"/>
        <property type="gene ID" value="ENSBIXG00005004388.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892646"/>
    <dbReference type="CTD" id="8664"/>
    <dbReference type="GeneTree" id="ENSGT00390000002667"/>
    <dbReference type="OMA" id="FMDKRDN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098808">
        <property type="term" value="F:mRNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002191">
        <property type="term" value="P:cap-dependent translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075522">
        <property type="term" value="P:IRES-dependent viral translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075525">
        <property type="term" value="P:viral translational termination-reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03003">
        <property type="entry name" value="eIF3d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007783">
        <property type="entry name" value="eIF3d"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05091">
        <property type="entry name" value="eIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016281">
        <property type="entry name" value="EIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="RNA gate" evidence="5">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="523"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="548" mass="63931" checksum="A477CE0A52508023" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IMA9</accession>
    <name>A0A4W2IMA9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor Maf</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MAF</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027369765.1">
        <property type="nucleotide sequence ID" value="XM_027513964.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027369766.1">
        <property type="nucleotide sequence ID" value="XM_027513965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IMA9"/>
    <dbReference type="SMR" id="A0A4W2IMA9"/>
    <dbReference type="STRING" id="30522.A0A4W2IMA9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008514.1">
        <property type="protein sequence ID" value="ENSBIXP00000004113.1"/>
        <property type="gene ID" value="ENSBIXG00000010465.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043532.1">
        <property type="protein sequence ID" value="ENSBIXP00005045339.1"/>
        <property type="gene ID" value="ENSBIXG00005001960.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875518"/>
    <dbReference type="CTD" id="4094"/>
    <dbReference type="GeneTree" id="ENSGT00940000161531"/>
    <dbReference type="OMA" id="GTVHPHM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070306">
        <property type="term" value="P:lens fiber cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030219">
        <property type="term" value="P:megakaryocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032330">
        <property type="term" value="P:regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14718">
        <property type="entry name" value="bZIP_Maf_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000016">
        <property type="entry name" value="MAF bZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013592">
        <property type="entry name" value="Maf_TF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08383">
        <property type="entry name" value="Maf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="292"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="358"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="317"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="387" mass="40125" checksum="58667FD6F8CEE28C" modified="2019-09-18" version="1">MASELAMSNSDLPTSPLAMEYVNDFDLMKFEVKKEPVETDRIISQCGRLIAGGSLSSTPMSTPCSSVPPSPSFSAPSPGSGSEQKAHLEDYYWMTGYPQQLNPEALGFSPEDAVEALISNSHQLQGGFDGYARGAQQLASAAGAGAGASLGGSGEEMGPAAAVVSAVIAAAAAQSGAAPHYHHHHHHHHAAGHHHHPTAGAPGAAGSASASAGGAGGSGGGSGGPASAGGGGGGGGGGGGGAAGAGGALHPHHAAAGGLHFDDRFSDEQLVTMSVRELNRQLRGVSKEEVIRLKQKRRTLKNRGYAQSCRFKRVQQRHVLESEKNQLLQQVDHLKQEISRLVRERDAYKEKYEKLVSSGFRENGSSSDNPSSPEFFMYPRESSTSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IN64</accession>
    <name>A0A4W2IN64_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GALNT17</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_027382566.1">
        <property type="nucleotide sequence ID" value="XM_027526765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IN64"/>
    <dbReference type="SMR" id="A0A4W2IN64"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014415.1">
        <property type="protein sequence ID" value="ENSBIXP00000001247.1"/>
        <property type="gene ID" value="ENSBIXG00000028757.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053831.1">
        <property type="protein sequence ID" value="ENSBIXP00005045148.1"/>
        <property type="gene ID" value="ENSBIXG00005002170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883233"/>
    <dbReference type="CTD" id="64409"/>
    <dbReference type="GeneTree" id="ENSGT00940000156014"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23474">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000192">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000203">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF38">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="465"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="598" mass="67778" checksum="6A7823C5785F56C5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BKJ3</accession>
    <name>A0A4W2BKJ3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BKJ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053339.1">
        <property type="protein sequence ID" value="ENSBIXP00000000337.1"/>
        <property type="gene ID" value="ENSBIXG00000029944.1"/>
    </dbReference>
    <dbReference type="OMA" id="CEINDSH"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Similar to preferentially-expressed antigen in melanoma-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="LRRC14/PRAME"/>
    </dbReference>
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        <property type="entry name" value="MELANOMA ANTIGEN PREFERENTIALLY EXPRESSED IN TUMORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224">
        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="282" mass="31369" checksum="98DBF3BB876BF660" modified="2019-09-18" version="1">IFTMPMKSIRKILKVVQLDSIQDLEVNCIWKLATLSRFLPHLGRMGNLRRLLLSENCVNQLSAQFLNLPHLQELYLDSISFLEGRLHQVLRCLNTPLETLSITNCLISESDLTYLSQCPSVSLLKDLGLSGVNLTSLNLESLQVLIERTSATLQELDLDECGIMDSQFSALLPSLSGCSQLTTFSFCGNPISMAVLESLLHHTMGLSKLSHVLYPAPLESYEDVHGTLHLGLLAQLHARLKQLLCKSGRSSMFWVSASPCPHCGDQILYDTEPILCPCYLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNF8</accession>
    <name>A0A4W2BNF8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Integrin subunit alpha X</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ITGAX</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNF8"/>
    <dbReference type="STRING" id="30522.A0A4W2BNF8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013844.1">
        <property type="protein sequence ID" value="ENSBIXP00000001520.1"/>
        <property type="gene ID" value="ENSBIXG00000008085.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01469">
        <property type="entry name" value="vWA_integrins_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000005">
        <property type="entry name" value="Integrin alpha L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000009">
        <property type="entry name" value="Integrin alpha M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1530:FF:000003">
        <property type="entry name" value="Integrin alpha M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000004">
        <property type="entry name" value="Integrin subunit alpha M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000001">
        <property type="entry name" value="Integrin, alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF118">
        <property type="entry name" value="INTEGRIN ALPHA-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="INTEGRIN_ALPHA"/>
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    </dbReference>
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        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0401">Integrin</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021509260" evidence="17">
        <location>
            <begin position="26"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1113"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="19">
        <location>
            <begin position="159"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="347"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="451"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="514"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="577"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1150"/>
            <end position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1171" mass="128825" checksum="2552B4100EE83873" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQA9</accession>
    <name>A0A4W2BQA9_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
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        <property type="gene ID" value="ENSBIXG00000029110.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:iron ion transport"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Ferritin_DPS_dom"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FERRITIN_LIKE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="4"/>
            <end position="153"/>
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            <position position="21"/>
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        <ligand>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="176" mass="20101" checksum="CA716B146E6662FB" modified="2019-09-18" version="1">MERQNYRPECEAAINNHATLEFHASFQCLAVALYLDHDDVGLKHFSHFFLLRSQEHSRTAESLMFLQIQRGGRICFLNIGKPETQQWKSGLQAMQDSLHWEKYVNQSLLNLHQLATDSSDANLCHFLGTGYLDQQVRFIKELGDHVSNLSNMGSPEGALEEDFFDKLTLGDGDKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQF3</accession>
    <name>A0A4W2BQF3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Stress-associated endoplasmic reticulum protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with SEC61B, SEC61A1 and the SEC61 complex. Interacts with CANX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAMP4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQF3"/>
    <dbReference type="STRING" id="30522.A0A4W2BQF3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012296.1">
        <property type="protein sequence ID" value="ENSBIXP00000002243.1"/>
        <property type="gene ID" value="ENSBIXG00000008726.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010580">
        <property type="entry name" value="ER_stress-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601">
        <property type="entry name" value="STRESS ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN SERP1/RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601:SF20">
        <property type="entry name" value="STRESS-ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06624">
        <property type="entry name" value="RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="30634" checksum="F255EEE2687140A9" modified="2019-09-18" version="1">RRSKLPRVVQVTHVRAVTTKLRLGSKPKSFLHVSLRCGAVRLPSNPVTAQAGRPLSAPSSHPLLPILGKIRSFLQHFRKLLLRPSLRKPFPRPPKGRLLPTVPQKRGPSPVLPRSTCLLGTAGRRLQPCPRPAGSGAGEHDPGSKRSRPNPFPRASVGPGPGSPARSERTKPAAAGTLGLAAFGRWIPTPPGSPDRSRSARCAPAAGWESRAFPGARAMVAKQRIRMANEKHSKNITQRGNVAKTLRPQEEKYPVGPWLLALFVFVVCGSAIFQIIQSIRMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRE1</accession>
    <name>A0A4W2BRE1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRE1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013353.1">
        <property type="protein sequence ID" value="ENSBIXP00000001731.1"/>
        <property type="gene ID" value="ENSBIXG00000008270.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="343" mass="39785" checksum="EA13347FDA85C75B" modified="2019-09-18" version="1">MLKILQARLQPYVNRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKRVCQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIGGRNINNLRYADDTTLMAESEEELKSLLMKVKVENEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKTMVFPVVMCGCESWTVRKAEHRRIDAFELWCWIKLLRVPWTARRSNQSILKEISHEYSLERLMLKLKLQYFGYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRS1</accession>
    <name>A0A4W2BRS1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRS1"/>
    <dbReference type="STRING" id="30522.A0A4W2BRS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013667.1">
        <property type="protein sequence ID" value="ENSBIXP00000001590.1"/>
        <property type="gene ID" value="ENSBIXG00000008129.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031005">
        <property type="term" value="F:filamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24207:SF1">
        <property type="entry name" value="FILAMIN-BINDING LIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24207">
        <property type="entry name" value="ZYX102 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="21353" checksum="6E4FBCB9E1F1E364" modified="2019-09-18" version="1">MASKPEKRVASSVFITLVPPRRDEAVVEEVRRAACEAQPGHPWESPAPTKAPGAGSVGKLRSWMPPGRTAAPGPAVPPPLSNGGPGGGASTAASAQSSQARRGGAAAHHSKEPVGFPERDICAFCRKTVSPRELGVEAMERQYPPSASRAVSAANGWPGRASTRRMGGPSVKPATRTPGEVCQVCKVVRERIIRALGQAFHPSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWR8</accession>
    <name>A0A4W2BWR8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA-binding protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RNA-binding motif protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RBM14</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027388354.1">
        <property type="nucleotide sequence ID" value="XM_027532553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BWR8"/>
    <dbReference type="SMR" id="A0A4W2BWR8"/>
    <dbReference type="STRING" id="30522.A0A4W2BWR8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009327.1">
        <property type="protein sequence ID" value="ENSBIXP00000003711.1"/>
        <property type="gene ID" value="ENSBIXG00000010111.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024572.1">
        <property type="protein sequence ID" value="ENSBIXP00005036675.1"/>
        <property type="gene ID" value="ENSBIXG00005018284.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886383"/>
    <dbReference type="CTD" id="10432"/>
    <dbReference type="GeneTree" id="ENSGT00940000157436"/>
    <dbReference type="OMA" id="IMSKRYG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990935">
        <property type="term" value="F:splicing factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098534">
        <property type="term" value="P:centriole assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046600">
        <property type="term" value="P:negative regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902151">
        <property type="term" value="P:regulation of response to DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12608">
        <property type="entry name" value="RRM1_CoAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12609">
        <property type="entry name" value="RRM2_CoAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000046">
        <property type="entry name" value="RNA-binding protein 14 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034506">
        <property type="entry name" value="RBM14_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034507">
        <property type="entry name" value="RBM14_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="79"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="193"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="566"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="669" mass="69575" checksum="65B74C3813B78D2F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYW7</accession>
    <name>A0A4W2BYW7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYW7"/>
    <dbReference type="STRING" id="30522.A0A4W2BYW7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005426.1">
        <property type="protein sequence ID" value="ENSBIXP00000005589.1"/>
        <property type="gene ID" value="ENSBIXG00000011756.1"/>
    </dbReference>
    <dbReference type="OMA" id="ANQERIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:mitotic spindle assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="KISc_BimC_Eg5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047241">
        <property type="entry name" value="KIF11-like_kin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF26">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="42"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="129"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="374" mass="41529" checksum="06DA16D6B05D9B81" modified="2019-09-18" version="1">MAISESAPVPGRGGGRPAPWPGLLWGRSWHLSRAHLQGWAGSIQVVVRCRPFNLAERKANAHSVVEWDHVWKEVSVRTGGLADKSSRETYTFDTVFGANTKQIDIYRSVVCPILDEVIMGYNCTIFAYGQTHAGKTFTMEEEDPLAGIIPHTLHQIFEKLTDNGTELSVKVLLLEIYNEELFYLLSLSSDVSESLQMFDDPHNKRGVIIKGLEEITVSNKDEVYQILEKGAAKRTAFVKTGKLNLVDLAVSENIGHSGAVDKRAREAGNINQSLLTLGRVIIALVERTPHVPYREPKLTRILQDSLGGHTRTSIIATVSPASLNLEETLSTLEYAHRAKNILNKPDINQKLTKKVLIKEYTEERTSETGSSCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZP7</accession>
    <name>A0A4W2BZP7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Interleukin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the IL-15/IL-21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZP7"/>
    <dbReference type="SMR" id="A0A4W2BZP7"/>
    <dbReference type="STRING" id="30522.A0A4W2BZP7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007311.1">
        <property type="protein sequence ID" value="ENSBIXP00000004681.1"/>
        <property type="gene ID" value="ENSBIXG00000010995.1"/>
    </dbReference>
    <dbReference type="OMA" id="MIHQHLR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051251">
        <property type="term" value="P:positive regulation of lymphocyte activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045954">
        <property type="term" value="P:positive regulation of natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.70:FF:000002">
        <property type="entry name" value="Interleukin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.70">
        <property type="entry name" value="Interleukin-15/Interleukin-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003443">
        <property type="entry name" value="IL-15/IL-21_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14356">
        <property type="entry name" value="INTERLEUKIN-15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14356:SF2">
        <property type="entry name" value="INTERLEUKIN-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02372">
        <property type="entry name" value="IL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021360891" description="Interleukin" evidence="9">
        <location>
            <begin position="25"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5F3</accession>
    <name>A0A4W2C5F3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lysosomal alpha-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Acid maltase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GAA</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5F3"/>
    <dbReference type="SMR" id="A0A4W2C5F3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000655.1">
        <property type="protein sequence ID" value="ENSBIXP00000008007.1"/>
        <property type="gene ID" value="ENSBIXG00000013864.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.110.10:FF:000007">
        <property type="entry name" value="Lysosomal alpha-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000072">
        <property type="entry name" value="lysosomal alpha-glucosidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000005">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017957">
        <property type="entry name" value="P_trefoil_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF92">
        <property type="entry name" value="LYSOSOMAL ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00025">
        <property type="entry name" value="P_TREFOIL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021439576" description="Lysosomal alpha-glucosidase" evidence="17">
        <location>
            <begin position="27"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="18">
        <location>
            <begin position="68"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="937" mass="104757" checksum="B7E9A2D66BAF5026" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6C2</accession>
    <name>A0A4W2C6C2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SETD3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-histidyl-[protein] + S-adenosyl-L-methionine = N(tele)-methyl-L-histidyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19369"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:11600"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16367"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.85"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with MYOD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027377779.1">
        <property type="nucleotide sequence ID" value="XM_027521978.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027377780.1">
        <property type="nucleotide sequence ID" value="XM_027521979.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027377782.1">
        <property type="nucleotide sequence ID" value="XM_027521981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6C2"/>
    <dbReference type="SMR" id="A0A4W2C6C2"/>
    <dbReference type="STRING" id="30522.A0A4W2C6C2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015960.1">
        <property type="protein sequence ID" value="ENSBIXP00000008339.1"/>
        <property type="gene ID" value="ENSBIXG00000001623.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022201.1">
        <property type="protein sequence ID" value="ENSBIXP00005012702.1"/>
        <property type="gene ID" value="ENSBIXG00005016900.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880016"/>
    <dbReference type="CTD" id="84193"/>
    <dbReference type="GeneTree" id="ENSGT00940000153577"/>
    <dbReference type="OMA" id="QHIDGIF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19176">
        <property type="entry name" value="SET_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000001">
        <property type="entry name" value="histone-lysine N-methyltransferase setd3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000001">
        <property type="entry name" value="histone-lysine N-methyltransferase setd3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025785">
        <property type="entry name" value="SETD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044428">
        <property type="entry name" value="SETD3_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51565">
        <property type="entry name" value="SAM_MT85_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="11">
        <location>
            <begin position="101"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="555"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="570"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="601" mass="67807" checksum="5B3EAC13853636B5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6S0</accession>
    <name>A0A4W2C6S0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Leucine rich repeats and transmembrane domains 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LRTM2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6S0"/>
    <dbReference type="SMR" id="A0A4W2C6S0"/>
    <dbReference type="STRING" id="30522.A0A4W2C6S0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002186.1">
        <property type="protein sequence ID" value="ENSBIXP00000007267.1"/>
        <property type="gene ID" value="ENSBIXG00000013231.1"/>
    </dbReference>
    <dbReference type="OMA" id="QISWIAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000015">
        <property type="entry name" value="Leucine rich repeat containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF5">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021212645" evidence="17">
        <location>
            <begin position="38"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="18">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="19">
        <location>
            <begin position="202"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="262"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="374" mass="41108" checksum="FC2B5881E2B263A2" modified="2019-09-18" version="1">MLAPGGSPEPRGTKGHTWLALWRRISWLACWIALCTAEAVPACPFPCTCDGRGLQVDCSGLGLTAPPPDLPAATRSLLLLNNRLSSLPGGAFANLSGLQRLDLSNNFLDRLPRMAFGDLANLTELQLRNNSLRALDATLLRPLPRLRHLDLSLNGLSRLPPGLFDGLPALRSLSLRANRLQSLDRRTFEPLAGLQLLQVADNPWECDCHLRDFKRWLEWFSYRGGRLDQLACTLPKELRGKDMRMVPMEMFNYCSQLEDQNSSAGQEVSGPPCTKASPEPAKPKPGPEPEPEPSTACPQKQRPRPVSVRRAIGTVIIAGVVCGVVCIMMVVAAAYGCIYASLMAKYHRELKKRQPLMGDPEGEQEDQKQISSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8T8</accession>
    <name>A0A4W2C8T8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ubiquitin carboxyl-terminal hydrolase 32</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Deubiquitinating enzyme 32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Ubiquitin thioesterase 32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Ubiquitin-specific-processing protease 32</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Deubiquitinase that can remove conjugated ubiquitin from target proteins, such as RAB7A and LAMTOR1. Acts as a positive regulator of the mTORC1 signaling by mediating deubiquitination of LAMTOR1, thereby promoting the association between LAMTOR1 and the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 31">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Golgi apparatus membrane</location>
            <topology evidence="19">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011339.1">
        <property type="protein sequence ID" value="ENSBIXP00000002712.1"/>
        <property type="gene ID" value="ENSBIXG00000009192.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021204233" evidence="9">
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            <end position="1847"/>
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    <feature type="domain" description="Fibrillar collagen NC1" evidence="10">
        <location>
            <begin position="1618"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <end position="279"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="526"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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    <sequence length="1847" mass="184867" checksum="AC6C269EA2623CFC" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
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    </dbReference>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010408.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="149" mass="16978" checksum="28F8B67FBAF56BC9" modified="2019-09-18" version="1">MSFLLPKLSSKKAVDQAIKSTAEKVLVLRFGRDEDPVCLQLDDILSKTSSDLSKMAAIYLVDVDQTPVYTHYFDISYIPSTVFFFNGQHMKVDYGSPDHTKFVGSFKTKQDFIDLIEVIYRGAMRGKLIVQSPIDPKNIPKYDLLYQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFM4</accession>
    <name>A0A4W2CFM4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CFM4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006472.1">
        <property type="protein sequence ID" value="ENSBIXP00000005091.1"/>
        <property type="gene ID" value="ENSBIXG00000011337.1"/>
    </dbReference>
    <dbReference type="OMA" id="PEHIWER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019068">
        <property type="term" value="P:virion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.375.10">
        <property type="entry name" value="Human Immunodeficiency Virus Type 1 Capsid Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003036">
        <property type="entry name" value="Gag_P30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008919">
        <property type="entry name" value="Retrov_capsid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050462">
        <property type="entry name" value="Retroviral_Gag-Pol_poly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33166">
        <property type="entry name" value="GAG_P30 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02093">
        <property type="entry name" value="Gag_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47943">
        <property type="entry name" value="Retrovirus capsid protein, N-terminal core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="396" mass="44765" checksum="2746D11AA93E7A82" modified="2019-09-18" version="1">MKELCLKYGIVVRPKSEPTGQMVLGTGNHEKEPPREGSPPTAPELPGAPSSYPNVPPYPGAPPPQKPARVCPLVETGGEFRPTRVHKPFSLLELRQIKQDLGSYTDDPGKYIDTFQHITLAFDLTWKDIMVIFSQTLSDPEHTRVLKEARRYATGLHMSSDRYPVGETAVPSSDPNWNYNDPEHIWERDHFLICVKAGLRAAQQKVISYARVSAITQEPSENPIAFLERLKEALQKFTNLDLDSYEGRVILKDKFLSQCASDIRIKLQQLQQQDPAASLDEMVQTATNTFYNREQEKEAKAQEKERKKETRHAQMLAALQRSPMANPESLKDKARDRCLICRQAGHWAKECPNHDKSPRTACHKCHQLEHWAALCPRDPGASRSSAKPALTMVQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIL5</accession>
    <name>A0A4W2CIL5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-sensitive inward rectifier potassium channel 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Inward rectifier K(+) channel Kir2.4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Potassium channel, inwardly rectifying subfamily J member 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCNJ14</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371068.1">
        <property type="nucleotide sequence ID" value="XM_027515267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIL5"/>
    <dbReference type="STRING" id="30522.A0A4W2CIL5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023042.1">
        <property type="protein sequence ID" value="ENSBIXP00000012900.1"/>
        <property type="gene ID" value="ENSBIXG00000017910.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051822.1">
        <property type="protein sequence ID" value="ENSBIXP00005023335.1"/>
        <property type="gene ID" value="ENSBIXG00005004458.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876257"/>
    <dbReference type="CTD" id="3770"/>
    <dbReference type="GeneTree" id="ENSGT01030000234586"/>
    <dbReference type="OMA" id="CHNGWAP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000063">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF40">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 18" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="5 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="21">
        <location>
            <begin position="51"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="22">
        <location>
            <begin position="196"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="398"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="17">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="434" mass="47655" checksum="EC1DD9BF3BE72B29" modified="2019-09-18" version="1">MGLARALRRLSGALESGDDRAGDEEEAGPGLCHNGWAPAQSPVGRRRGRFVKKDGHCNVRFVNLGGQGARYLSDLFTTCVDVRWRWMCLLFSCSFLASWLLFGLAFWLIASLHGDLTAPPPPAPCFSQVASFLAAFLFALETQTSIGYGVRSVTEECPAAVAAVVLQCIAGCVLDAFVVGAVMAKMAKPKKRNETLVFSENAVVALRDRRLCLMWRVGNLRRSHLVEAHVRAQLLQPRVTPEGEYIPLDHQDVDVGFDGGTDRIFLVSPITIVHEIDSASPLYELGRAELARADFELVVILEGMVEATAMTTQCRSSYLPGELLWGHRFEPVLFQRGSQYEVDYRHFHRTYEVPGTPVCSAKELDERAERASQSPKSGFPSSLAAFCYENELALSCCQEEDEEEEATEGDAAEAEDKAASPQVLTPTLALTLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIT9</accession>
    <name>A0A4W2CIT9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ARF interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARFIP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIT9"/>
    <dbReference type="SMR" id="A0A4W2CIT9"/>
    <dbReference type="STRING" id="30522.A0A4W2CIT9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004182.1">
        <property type="protein sequence ID" value="ENSBIXP00000006226.1"/>
        <property type="gene ID" value="ENSBIXG00000012349.1"/>
    </dbReference>
    <dbReference type="OMA" id="HRRHYER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07660">
        <property type="entry name" value="BAR_Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000003">
        <property type="entry name" value="arfaptin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF4">
        <property type="entry name" value="ARFAPTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="7">
        <location>
            <begin position="182"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="402" mass="44720" checksum="8759574C13DF5806" modified="2019-09-18" version="1">MSDCSNDTSTLGTSKPATEEVKSSSEESTMAQESPKNSAAEIPVTSNGEVDDSHEHGFNRDLKRSLPAGLGLSETQITSHGFDSTKEGVIEAGSFQGSPAPPLPSVMSPSRVAASRLTQQGSDLIVPAGSQRTQTKSGPVILADETKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJG9</accession>
    <name>A0A4W2CJG9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC113905276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJG9"/>
    <dbReference type="STRING" id="30522.A0A4W2CJG9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023521.1">
        <property type="protein sequence ID" value="ENSBIXP00000013219.1"/>
        <property type="gene ID" value="ENSBIXG00000018180.1"/>
    </dbReference>
    <dbReference type="OMA" id="KNICKRC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03598">
        <property type="entry name" value="CLECT_EMBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000090">
        <property type="entry name" value="Proteoglycan 2, bone marrow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033816">
        <property type="entry name" value="EMBP_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002352">
        <property type="entry name" value="Eosinophil_major_basic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00770">
        <property type="entry name" value="EMAJORBASICP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021412987" description="C-type lectin domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="104"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="220" mass="25161" checksum="29C0A29A9F3EFBF0" modified="2019-09-18" version="1">MKGCLLLLLLLLGTVSALYLENDAPHMGSPETQADLSQDAEGSGGQEGELALSGEVVESGGEEAEDAHDDEGDSESDPDDLDEDVQCPKEEETVQLPGGPECKRCYYRLVRTPRRFRHAQRICRRCYRGNLVSIHSYRFDYRVTRWSRRTNQSQVWIGGFIRGRSRCRRFRWTDGSRWNFGYWARGQPGNGRGHCITLCTRGGHWRRAPCKRRLPFICSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK55</accession>
    <name>A0A4W2CK55_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NOP58</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL or FBLL1 assemble stepwise onto the snoRNA. Interacts with NOLC1/Nopp140. Interacts with NOPCHAP1, NUFIP1, RUVBL1 and RUVBL2; NOPCHAP1 bridges the association of NOP58 with RUVBL1:RUVBL2 and NUFIP1. Interacts with PIH1D1. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CK55"/>
    <dbReference type="STRING" id="30522.A0A4W2CK55"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021873.1">
        <property type="protein sequence ID" value="ENSBIXP00000012149.1"/>
        <property type="gene ID" value="ENSBIXG00000017240.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056825.1">
        <property type="protein sequence ID" value="ENSBIXP00005046967.1"/>
        <property type="gene ID" value="ENSBIXG00005031577.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153534"/>
    <dbReference type="OMA" id="MGMRSNW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001094">
        <property type="term" value="F:TFIID-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000004">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nop56/Nop58"/>
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        <property type="entry name" value="NOP58/56_N"/>
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        <property type="entry name" value="Nop_C"/>
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        <property type="entry name" value="Nop_dom_sf"/>
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        <property type="entry name" value="NOSIC"/>
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    <dbReference type="PANTHER" id="PTHR10894">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
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    </dbReference>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="282"/>
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        </location>
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        <location>
            <begin position="466"/>
            <end position="531"/>
        </location>
    </feature>
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        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
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        <location>
            <begin position="487"/>
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        </location>
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        <location>
            <begin position="519"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00060303"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00063404"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="531" mass="59763" checksum="61477E91EDFC966F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMH1</accession>
    <name>A0A4W2CMH1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="RT_POL"/>
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        <location>
            <begin position="198"/>
            <end position="371"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="371" mass="42899" checksum="377CA227D77295C9" modified="2019-09-18" version="1">MEVRDIVQETGIKTIPMEKKFKKAKWLSGEALQIAVKRREAKSKGEKERYKHLNAEFQRIARRDKKAFFSNQCKEIEENNRMGKTRDLFKKIRDTKETFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHHGVITDLEPDILECEIKSALGSITTNKASGGDGIPVELFQILKDDAVKVLHSIVQQIWKTQQWPQDWKTSVFIPIPKQGNAKKCSNNRTIALISHTSKVMLKILQARLQQYVNCELTDVQAGFRKGRGTRDQIANICWIMEKAREFQKSIYFCFIDYAKAFDCVDHNKLWKILQEMGIPDHLISLLRNLYAGQEATVRTGHGTTDWFQLGKGVCQGCILSPCLFNLYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQR4</accession>
    <name>A0A4W2CQR4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQR4"/>
    <dbReference type="STRING" id="30522.A0A4W2CQR4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000015529.1"/>
        <property type="gene ID" value="ENSBIXG00000003616.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="entry name" value="SAM_Samd3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR042812">
        <property type="entry name" value="SAMD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042813">
        <property type="entry name" value="SAMD3_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47302">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47302:SF1">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM"/>
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    </dbReference>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="510" mass="59493" checksum="F2842EC30C760194" modified="2019-09-18" version="1">METWSVDQVCSWLVEKNLGELVHRFQEEEVSGAALLALNDRMVQQLVKKIGHQAVLMDLIKKYKQKSQELKSPGETAPVTQAEDEQPSSPASGGEQMPSFYPADNLDNGLIDHRVLKQRRNVRHILARNKALQWTKSYVLPEFPYDVKCMLAEQKCPDHSMRIRIIEFLQADMTKYLEGSLYPSTQQYNDVVNALLQAHPFLDEDGCGFFLWKRALKDRFKYVRRPIEDDEQVMRNKCKFGHRRGQTRKSLADIRFEIKTVQIKEEAVCLDPEVDEHINWFQQEYVKTEKDWREIDKRMSQTLEIRRKLIGGRTPLKDILKMFPFLQCPYQLFREFQLLTRTDIFKKTRHILESYSENILTSLSLVDNPIYIALQEKMEQHTDEDMLKHMKMTATCLLLPDVFGEDSSLFVIVNDKVQVSTPVLEVKNPFNMDVCEFSLYLEKERLTKVDDCITALAALIASFHVFGIECPRRLSQTLNFLETLIFDVHSPYSPSMKEKEKEVGFQHPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CS87</accession>
    <name>A0A4W2CS87_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with pre-60S ribosomal particles. Interacts with MINAS-60 (product of an alternative open reading frame of RBM10).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:ribosome biogenesis"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
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    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="9">
        <location>
            <begin position="227"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="604"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="651"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="677"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="692" mass="80118" checksum="AF2089F8B000CB74" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CT10</accession>
    <name>A0A4W2CT10_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113897838</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CT10"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026145.1">
        <property type="protein sequence ID" value="ENSBIXP00000014981.1"/>
        <property type="gene ID" value="ENSBIXG00000019565.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15430">
        <property type="entry name" value="7tmA_OR13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="34742" checksum="FE0812BE8BDA70C1" modified="2019-09-18" version="1">MERVNSTEVSEFFLKGFSGYPALEHLLFPLCSAMYLVTLLGNTGIVAVSVLDARLHTPMYFFLGNLSILDICYTSTFVPLMLVHLLSAQKTISFLGCALQMCLSLSTGSTECLLLTIMAYDRYLAICWPLRYPVLMSHRLCWLLAGTAWVLCLFKSVTETVIAMRLPFCGHRVVSHFTCEILAVLKLACGDTSISEVFLLVGAILLLPVPLAFICLSYTLILATTLRVPSAAGRRKAFSTCSAHLAVVMLFYGTVIFMYMKPKSKEARISDEIFTVLYAVVTPMLNPVIYSLRNKEVKEAARKVWGRIQTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTK1</accession>
    <name>A0A4W2CTK1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 67</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM67</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTK1"/>
    <dbReference type="STRING" id="30522.A0A4W2CTK1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017967.1">
        <property type="protein sequence ID" value="ENSBIXP00000009613.1"/>
        <property type="gene ID" value="ENSBIXG00000015301.1"/>
    </dbReference>
    <dbReference type="OMA" id="YITENKG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019170">
        <property type="entry name" value="Meckelin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274:SF2">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09773">
        <property type="entry name" value="Meckelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021208682" evidence="2">
        <location>
            <begin position="38"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="565"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="731"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="996" mass="111341" checksum="4C755F59FF242F16" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CU53</accession>
    <name>A0A4W2CU53_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DOP1 leucine zipper like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DOP1A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000018293.1">
        <property type="protein sequence ID" value="ENSBIXP00000009818.1"/>
        <property type="gene ID" value="ENSBIXG00000015434.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056459">
        <property type="entry name" value="TPR_DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF22">
        <property type="entry name" value="PROTEIN DOPEY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24601">
        <property type="entry name" value="TPR_DOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="319"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like TPR" evidence="11">
        <location>
            <begin position="1363"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="1904"/>
            <end position="2404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="577"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="632"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1107"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1168"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1236"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1281"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="633"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1108"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1131"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1196"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1236"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1281"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1305"/>
            <end position="1319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2465" mass="276389" checksum="9D943CBEC06FC5A8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUF1</accession>
    <name>A0A4W2CUF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin--tyrosine ligase-like protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUF1"/>
    <dbReference type="SMR" id="A0A4W2CUF1"/>
    <dbReference type="STRING" id="30522.A0A4W2CUF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047751.1">
        <property type="protein sequence ID" value="ENSBIXP00000016984.1"/>
        <property type="gene ID" value="ENSBIXG00000021054.1"/>
    </dbReference>
    <dbReference type="OMA" id="NDITMHL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF39">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="461" mass="54111" checksum="690D5290E5F2D6D5" modified="2019-09-18" version="1">MSRPKNQNYKGHGLQKGKEREQRASIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMGEHVRISHFRNHYELTRKNYMVKNLKRFRKQLEREAGKLEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWKKGTAGKKLTSLEAQPARNTVNPSGSHDTRSSDDQKDEIPVENYVAQRYIENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDQYVHLTNVAVQKTSPDYHPKKGCKWMLQRFRQYLASKHGPEAVETLFSDMDNIFIRSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHIVDMEARLTGREKRVGGFDLMWNDGPVSREEGGPDLSGMGNFVTNTHLGCVNDRKEQLRQLFRSLQGQKKTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0M7</accession>
    <name>A0A4W2D0M7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D0M7"/>
    <dbReference type="STRING" id="30522.A0A4W2D0M7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032819.1">
        <property type="protein sequence ID" value="ENSBIXP00000019281.1"/>
        <property type="gene ID" value="ENSBIXG00000004491.1"/>
    </dbReference>
    <dbReference type="OMA" id="FPFWYIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF42">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="104" mass="11505" checksum="21A35208565A7A56" modified="2019-09-18" version="1">GDSVNQTEGPVTLSEGALLTLNCTYQTTYSGSYHFWYVQHLNKAPQLLLKGSMTNQRPEHEGFQATLVRSDSSFHLQKRAVQASDSAVYYCALSDTVRKGCRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D283</accession>
    <name>A0A4W2D283_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transducin-like enhancer protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TLE6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimers. Component of the subcortical maternal complex (SCMC), at least composed of NLRP5, KHDC3, OOEP, and TLE6. Within the complex, interacts with NLRP5, KHDC3 and OOEP. The SCMC may facilitate translocation of its components between the nuclear and cytoplasmic compartments. As part of the SCMC interacts with the SCMC-associated protein ZBED3. As part of the SCMC interacts with the SCMC-associated protein NLRP4F. As part of the SCMC interacts with the SCMC-associated protein CFL1/Cofilin-1. Interacts with FOXG1/BF-1; the interaction inhibits TLE1 interaction with FOXG1/BF-1. Interacts with NFATC1. Interacts with PAX6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D283"/>
    <dbReference type="STRING" id="30522.A0A4W2D283"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033732.1">
        <property type="protein sequence ID" value="ENSBIXP00000019911.1"/>
        <property type="gene ID" value="ENSBIXG00000023366.1"/>
    </dbReference>
    <dbReference type="OMA" id="KEELPCA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140095">
        <property type="term" value="C:cytoplasmic lattice"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106333">
        <property type="term" value="C:subcortical maternal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140094">
        <property type="term" value="F:structural constituent of cytoplasmic lattice"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140089">
        <property type="term" value="P:protein storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000546">
        <property type="entry name" value="TLE family member 6, subcortical maternal complex member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF2">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="387"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="575" mass="64102" checksum="7D9A13C24AD3D403" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D373</accession>
    <name>A0A4W2D373_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Aspartate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="5">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">DARS2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the attachment of aspartate to tRNA(Asp) in a two-step reaction: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D373"/>
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        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
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        <property type="entry name" value="tRNA_anti-codon"/>
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        <property type="entry name" value="TRNASYNTHASP"/>
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    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
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    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="20">
        <location>
            <begin position="184"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054114"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="651" mass="73748" checksum="5E58AC3A2FE9A5D3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8X0</accession>
    <name>A0A4W2D8X0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SH3 domain-binding protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SH3BP4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer or homooligomer. Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC. Interacts with the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; the interaction is most probably direct, preferentially occurs with their inactive GDP-bound form and is negatively regulated by amino acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027393777.1">
        <property type="nucleotide sequence ID" value="XM_027537976.1"/>
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        <property type="nucleotide sequence ID" value="XM_027537977.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000022397.1"/>
        <property type="gene ID" value="ENSBIXG00000025256.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005033701.1">
        <property type="protein sequence ID" value="ENSBIXP00005040714.1"/>
        <property type="gene ID" value="ENSBIXG00005006169.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890200"/>
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    <dbReference type="GeneTree" id="ENSGT00390000013151"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061462">
        <property type="term" value="P:protein localization to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11757">
        <property type="entry name" value="SH3_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000117">
        <property type="entry name" value="SH3 domain-binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000008">
        <property type="entry name" value="SH3 domain-binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056183">
        <property type="entry name" value="DEATH_SH3BP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056181">
        <property type="entry name" value="SH3BP4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035456">
        <property type="entry name" value="SH3BP4_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056182">
        <property type="entry name" value="UPA_SH3BP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15603:SF3">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15603">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24094">
        <property type="entry name" value="DEATH_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23637">
        <property type="entry name" value="SH3BP4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23640">
        <property type="entry name" value="UPA_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="55"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="16">
        <location>
            <begin position="317"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="654"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="963" mass="107096" checksum="B9E434DE34926EEA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAY0</accession>
    <name>A0A4W2DAY0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipase A2</fullName>
            <ecNumber evidence="23">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC113879934</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45472"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72829"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45473"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:41232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40431"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73009"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40432"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73008"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40816"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40911"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoglycerol + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44524"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84472"/>
            <dbReference type="ChEBI" id="CHEBI:84475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:44525"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:78097"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:45425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + H2O = a 1-acyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:64381"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:44605"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="20">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 23">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAY0"/>
    <dbReference type="STRING" id="30522.A0A4W2DAY0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050864.1">
        <property type="protein sequence ID" value="ENSBIXP00000023212.1"/>
        <property type="gene ID" value="ENSBIXG00000025751.1"/>
    </dbReference>
    <dbReference type="OMA" id="GDQDYCK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000001">
        <property type="entry name" value="Basic phospholipase A2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF9">
        <property type="entry name" value="PHOSPHOLIPASE A2, MEMBRANE ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="20 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 23" id="KW-0964">Secreted</keyword>
    <keyword evidence="23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021511783" description="Phospholipase A2" evidence="23">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="24">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="46"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="69"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="144" mass="16551" checksum="3015D07999496431" modified="2019-09-18" version="1">MKTLLLLAVIMAIGLLQVHGGLLDFRKMIKFTTGKEPAISYGFYGCHCGVGRRGTPKDATDWCCRIHDCCYENLRKRGCRTSFQSYNFIFQRGQIVCGDQDYCKRQLCQCDKRAADCLDRNLKTYNKKLQYYNNLLCFGSTPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DD95</accession>
    <name>A0A4W2DD95_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Relaxin-3 receptor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 100</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Insulin-like peptide INSL5 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Relaxin family peptide receptor 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">High affinity receptor for INSL5. Also acts as a receptor for RLN3/relaxin-3, as well as bradykinin and kallidin. Binding of the ligand inhibit cAMP accumulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DD95"/>
    <dbReference type="STRING" id="30522.A0A4W2DD95"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051355.1">
        <property type="protein sequence ID" value="ENSBIXP00000024110.1"/>
        <property type="gene ID" value="ENSBIXG00000026365.1"/>
    </dbReference>
    <dbReference type="OMA" id="WVLGNCA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000273">
        <property type="entry name" value="Relaxin family peptide/INSL5 receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000248">
        <property type="entry name" value="ATII_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF951">
        <property type="entry name" value="RELAXIN FAMILY PEPTIDE_INSL5 RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00241">
        <property type="entry name" value="ANGIOTENSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="55"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00084004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="373" mass="40987" checksum="406E4C18B16085AF" modified="2019-09-18" version="1">MPTPNTSAPLPAFWVNASGGSVLSAADATMPVGFLALRVSVALAYGLVGAVGLLGNSAVLWVLGNCAQRAPCPPSDTFVFNLALADLGLALTLPFWAAESALDFHWPFGGALCKMVLTATVLNIYASIFLITALSVARYWVVAMAAGPGTHLSLFWARVATLAMWVAASLVTVPTAVFGAEGEVSGVRLCLLRFPSRYWLGAYQLQRVVLAFMVPLSIITTSYLLLLAFLRRRRRRWRDSRGVAHSIHILLASFFLCWFPNHVVTLWGVLVKFDLVPWDSTFYTVHTYVFPVTTCLAHTNSCLNPVLYCLLRQEPRRALADTFRDLRARLWPQGRGWVEQVALKEMGRRWTESTPQEGGLSTMLTNLDKGNPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DE35</accession>
    <name>A0A4W2DE35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAM151A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC113889842</name>
        <name evidence="10" type="synonym">FAM151A</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the menorin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DE35"/>
    <dbReference type="STRING" id="30522.A0A4W2DE35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051565.1">
        <property type="protein sequence ID" value="ENSBIXP00000024407.1"/>
        <property type="gene ID" value="ENSBIXG00000026570.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046394.1">
        <property type="protein sequence ID" value="ENSBIXP00005035800.1"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF10223"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024407.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="586" mass="64006" checksum="1B908056F6EA56C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEW6</accession>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <location>
            <begin position="117"/>
            <end position="385"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="503" mass="58164" checksum="6B80F173CD88B33F" modified="2019-09-18" version="1">MGKTRDICKKIRDTKGTFHAKMSSIKDRNGMDLTEAEDIKKRWQEYTEELYKKNLHDPDNHNGVITDLEPDILECEVKWALESITTNKGSGGDGIPVELFQILKDDAVKVLHSICQQICKSQHWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYVNRECPDVQAGFRKGRGTRDQIAKIRWIIEKAREFQKNIYFCFLDYGKAFDCVDHNKLWKILQDMGIPDHLLCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSLCLFNFYAEYIMRNAGLEETQTGIKISWRNINNLRYADDTTLMTESEEELKSLLMKVKVESEEVGLKPNIQKTKIMASSPTTSWEIDEQTVEIVSDFIFLGSKITADGDCSHEIKRHLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAEHRRFDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIQ0</accession>
    <name>A0A4W2DIQ0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3 6">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GPX7</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
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            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glutathione peroxidase family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSBIXT00000040153.1">
        <property type="protein sequence ID" value="ENSBIXP00000024174.1"/>
        <property type="gene ID" value="ENSBIXG00000005531.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005034509.1"/>
        <property type="gene ID" value="ENSBIXG00005005715.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:response to oxidative stress"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPX_CS"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF5">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
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    </dbReference>
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        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="245" mass="26866" checksum="BFC5895BDEA2408E" modified="2019-09-18" version="1">MGRPRSRARRSTALGEEGGASCLEGRVAPGPPPPASRPRCARAPCPASALATPPAAERVMVAARAAAWLLLAAAACAPREQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTETSGKEPTWNFWKYLVAPDGKVIGAWDPTVSVEEIRPQITALVRKLILKKREDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLW0</accession>
    <name>A0A4W2DLW0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor AP-2-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">AP-2 transcription factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Activating enhancer-binding protein 2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Activator protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TFAP2A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
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        <property type="gene ID" value="ENSBIXG00000008242.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:regulation of cell population proliferation"/>
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    </dbReference>
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        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-ALPHA"/>
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        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2ATNSCPFCT"/>
    </dbReference>
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        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="207"/>
            <end position="401"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="410"/>
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        </location>
    </feature>
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            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
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        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="410"/>
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        </location>
    </feature>
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        </location>
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            <dbReference type="Ensembl" id="ENSBIXP00000027414.1"/>
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        <source>
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    <sequence length="433" mass="47362" checksum="C2D318FCD5F82D88" modified="2019-09-18" version="1">MSILAKMGDWQDRHDGASNGTARLPQLGTVGQSPYTSAPPLSHTPNADFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQSQESGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHGLGSGLGDLPIHSLPHAIEDVPHVEDPGINIPDQTVIKKGPVSLSKSNSNAVSAIPINKDNLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVTRKNMLLATKQICKEFTDLLAQDRSPLGNSRPNPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNSHTDNNAKSSDKEEKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM17</accession>
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        <name evidence="2" type="primary">OSTN</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM47</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    </dbReference>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="151" mass="16914" checksum="A91BD8D07A58E256" modified="2019-09-18" version="1">MAKMSFSSYILILTFSLLSQGISLSASKSIRNLDDDMVFKTLRLGKAFQKEDTAEKSIVVPSLEQYKNDESSFMNDEENKNSKNAGSKHNFLNHGLPLNLAIKPYLALKGSVAFPAENEVQNTESTQEKREIGDEENSAKFPIGRRDFDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DPR2</accession>
    <name>A0A4W2DPR2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
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        <name type="common">Hybrid cattle</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DPR2"/>
    <dbReference type="STRING" id="30522.A0A4W2DPR2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010201.1">
        <property type="protein sequence ID" value="ENSBIXP00000028472.1"/>
        <property type="gene ID" value="ENSBIXG00000009670.1"/>
    </dbReference>
    <dbReference type="OMA" id="CVSEFVF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15936">
        <property type="entry name" value="7tmA_OR4D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="310" mass="34887" checksum="1AC748047E0CE8E2" modified="2019-09-18" version="1">MQPGNLTCVSEFVFLGLSQTHKLHLFLFLVFLLVYTTTVMGNLLIMVTVSSDSRLHTPMYFLLRNLAVIDLCFSSVTAPKMLVDFLAEKKTISYQGCMTQIFFFHFLGGAMVFFLSVMAYDRLVAISRPLHYVTIMNTQLCVGLVVASWVGGFIHSISQLALMLPLPFCGPNVLDNFYCDVPQVLRLACTDTSVLESLVISNSGMLDVIWFLLLLVSYSAILVMLRSHSGQARRKAASTCTTHIIVMSMVFIPSIYLYARPFTSFSMDKAVSISHTVMTPMLNPMIYTLRNQEMQAAVKRLGRHWLLCKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQS6</accession>
    <name>A0A4W2DQS6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leprecan-like alpha-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leprecan family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQS6"/>
    <dbReference type="STRING" id="30522.A0A4W2DQS6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009710.1">
        <property type="protein sequence ID" value="ENSBIXP00000028632.1"/>
        <property type="gene ID" value="ENSBIXG00000000487.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001015">
        <property type="entry name" value="Cartilage associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052284">
        <property type="entry name" value="Collagen_mod_leprecan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13986:SF3">
        <property type="entry name" value="CARTILAGE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13986">
        <property type="entry name" value="PROTEIN LYSINE HYDROXYLATION COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021386705" description="Leprecan-like alpha-helical domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Leprecan-like alpha-helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="398" mass="44636" checksum="1FA9E7C3B98BEC28" modified="2019-09-18" version="1">MEPGRRAAAALLALLCSVCALHSGRAQYEALQLPAGRADAARVGLPARAGPVQQRALGGERGLLGDQPAAAPPATGQRGLLPPQLQRGPAARAHRRPRALPGVAPLRGPAAPSALPQTLQAGPAGLPPVETQPRSTGGLQRREPYKFLQFAYFKANNLPKAIAAAHTFLLKHPDDEMMKRNMAYYKSLPDAEDYIKDLETKSYESLFIRAVRAYNGENWRTCITDMELALPDFFKAFYECLAACEGSREIRDFKDFYLSIADHYVEVLECKIQCEENLTPVIGGYPVEKFVATMYHYLQFAYYKLNDLKNAAPCAVSYLLFDQSDKVMQQNLEYYQYHRDKWALADEHFQPRPEAVQFFNVTMLQKELYDFAKENLMDDDEGEVLEYVDDLLELGETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRA8</accession>
    <name>A0A4W2DRA8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GPX1</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:50708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57444"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:90680"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:50709"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16834"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer. Interacts with MIEN1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRA8"/>
    <dbReference type="STRING" id="30522.A0A4W2DRA8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008326.1">
        <property type="protein sequence ID" value="ENSBIXP00000029062.1"/>
        <property type="gene ID" value="ENSBIXG00000010559.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004676.1">
        <property type="protein sequence ID" value="ENSBIXP00005006283.1"/>
        <property type="gene ID" value="ENSBIXG00005011815.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156150"/>
    <dbReference type="OMA" id="RDYTEMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097413">
        <property type="term" value="C:Lewy body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060055">
        <property type="term" value="P:angiogenesis involved in wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051702">
        <property type="term" value="P:biological process involved in interaction with symbiont"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043534">
        <property type="term" value="P:blood vessel endothelial cell migration"/>
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    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:endothelial cell development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
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        <property type="term" value="P:fat cell differentiation"/>
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        <property type="term" value="P:fibroblast proliferation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:glutathione metabolic process"/>
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        <property type="term" value="P:negative regulation of release of cytochrome c from mitochondria"/>
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        <property type="term" value="P:neuron apoptotic process"/>
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        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="term" value="P:regulation of mammary gland epithelial cell proliferation"/>
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        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
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        <property type="term" value="P:response to gamma radiation"/>
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        <property type="term" value="P:response to hydrogen peroxide"/>
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        <property type="term" value="P:response to lipopolysaccharide"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:response to selenium ion"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0009609">
        <property type="term" value="P:response to symbiotic bacterium"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:response to xenobiotic stimulus"/>
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        <property type="term" value="P:sensory perception of sound"/>
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        <property type="term" value="P:skeletal muscle fiber development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:skeletal muscle tissue regeneration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:triglyceride metabolic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:UV protection"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:vasodilation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutaredoxin"/>
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        <property type="entry name" value="Glutathione_peroxidase"/>
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        <property type="entry name" value="GPX_CS"/>
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        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
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    <dbReference type="PANTHER" id="PTHR11592:SF41">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
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        <property type="entry name" value="Glutathion_perox"/>
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        <property type="entry name" value="GLUTPROXDASE"/>
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    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
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    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00036108"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00036482"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00038541"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029062.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="204" mass="22509" checksum="7557053B07BDA839" modified="2019-09-18" version="1">MCAAQRSAAALAAAAPRTVYAFSARPLAGGEPFNLSSLRGKVLLIENVASLGTTVRDYTQMNDLQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNCLKYVRPGGGFEPNFMLFEKCEVNGEKAHPLFAFLREVLPTPSDDATALMTDPKFITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFLTIDIEPDIETLLSQGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRX5</accession>
    <name>A0A4W2DRX5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000036632.1">
        <property type="protein sequence ID" value="ENSBIXP00000021768.1"/>
        <property type="gene ID" value="ENSBIXG00000006075.1"/>
    </dbReference>
    <dbReference type="OMA" id="NARANCM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="120"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="387" mass="44922" checksum="81F72A9683158777" modified="2019-09-18" version="1">MTDPSAFNSTLFSPTDEMQRTIIKDYTEIQRILRDYYQQLYANKMDNLEEIDKFLEKYNFPKRNQEEIENLNRPITSMEIETVITNLPANKSPGPDGFTAEFYQKFREELTSILLKLFRKSAEEGKLPNSFYEATITLIPKPDKDATKKENYRPISLMNIDAKILNKILAIRIQQHIKKIIHHDQVGFIPAMQGFFNICKLINVIHHINKLKNKSHMIISIDAEKAFDKIQHPFMIKTLQKAGIEGTYLNIIKAIYDKPTANIILKGEKLKAFSLKSGTRQGCPLSPLLFNIVLEVLATAIRAEKEIKGIQIGKEEVKLSLFADDMILYIENPKDSTRKLLQLINEYSKVAGYKINHRNPLHSYTLIMRKQKEKLRKQFHSPLQRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZR3</accession>
    <name>A0A4W2DZR3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small glutamine-rich tetratricopeptide repeat-containing protein beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-SGT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SGTB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homooligomerize.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376401.1">
        <property type="nucleotide sequence ID" value="XM_027520600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZR3"/>
    <dbReference type="SMR" id="A0A4W2DZR3"/>
    <dbReference type="STRING" id="30522.A0A4W2DZR3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017338.1">
        <property type="protein sequence ID" value="ENSBIXP00000032324.1"/>
        <property type="gene ID" value="ENSBIXG00000014868.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017943.1">
        <property type="protein sequence ID" value="ENSBIXP00005009812.1"/>
        <property type="gene ID" value="ENSBIXG00005014614.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879268"/>
    <dbReference type="CTD" id="54557"/>
    <dbReference type="GeneTree" id="ENSGT00940000158321"/>
    <dbReference type="OMA" id="DMARNMM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000002">
        <property type="entry name" value="Small glutamine-rich tetratricopeptide repeat-containing protein alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000103">
        <property type="entry name" value="small glutamine-rich tetratricopeptide repeat-containing protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032374">
        <property type="entry name" value="SGTA_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF1">
        <property type="entry name" value="SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16546">
        <property type="entry name" value="SGTA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SGTA homodimerisation" evidence="12">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="304" mass="33453" checksum="2CAAA1DDBFB45CFD" modified="2019-09-18" version="1">MSSIKHLVYAVIRFLREQSQMDAYTSDEQESLEVAIQCLETVFKISAEDTHLAVSQPLTEMFTNSFCKNDILPLSNSVPEDMGKADQLKDEGNNHMKEENYAAAVDCYTQAIELDSNNAVYYCNRAAAQSKLGHYTDAIKDCEKAIAIDSKYSKAYGRMGLALTAMNKFQEAVTSYQKALDLDPENDSYKSNLKIAEQKLREVSSPTGTGLSFDMASLINNPAFISMAASLMQNPQVQQLMSGMMTNAIGGPAAGVGGLTDLSSLIQAGQQFAQQIQQQNPELIEQLRNHIRSRSFSSSAEEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E032</accession>
    <name>A0A4W2E032_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 36 member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC36A3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E032"/>
    <dbReference type="STRING" id="30522.A0A4W2E032"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041364.1">
        <property type="protein sequence ID" value="ENSBIXP00000024951.1"/>
        <property type="gene ID" value="ENSBIXG00000005688.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030635.1">
        <property type="protein sequence ID" value="ENSBIXP00005039346.1"/>
        <property type="gene ID" value="ENSBIXG00005003667.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162406"/>
    <dbReference type="OMA" id="NQMKNPQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005280">
        <property type="term" value="F:amino acid:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015180">
        <property type="term" value="F:L-alanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015193">
        <property type="term" value="F:L-proline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF258">
        <property type="entry name" value="PROTON-COUPLED AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="43"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="471" mass="52387" checksum="665368B8099141F7" modified="2019-09-18" version="1">ISLLARDNTSKPKSLDDGSKSLSESSSSITSERIHPAEEANGLSMMQTLIHLLKCNIGTGLLGLPLAMKNAGLLVGPFSLLAIGILTVHCMVILLNCAHHLSQRLQKTFVNYGEAMMYSLETCPNAWLRTHSVWGRYTVSFLLITTQLGFCSVYFMFMADNLQQMVEEVHVTSKTCEPRKILVLTPNVDIRFYMLTILPFLILLVFIQNLRVLSIFSTLANITTLGSMALIFQYIMQEIPDPRNLPLMASWKTFLLFFGTAIFTFEGVGMVLPLKNQMKHPQQFSFVLYWGMSLVIVLYICLGTLGYMKFGSNTQASITLNLPNCWLYQSVKLMYSIGIFFTYALQFHVPAEIIIPVIISQVSESWALFADLSVRTALVCLTCVSAILIPRLDLVISLVGSVSSSALALIIPPFLELITFYPEDMNCITIVKDIMISILGLLGCVFGTYQALYELTQPINHSVANSTGVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1T6</accession>
    <name>A0A4W2E1T6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger and BTB domain-containing protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZBTB32</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16. Interacts with GATA3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027370101.1">
        <property type="nucleotide sequence ID" value="XM_027514300.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027370104.1">
        <property type="nucleotide sequence ID" value="XM_027514303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E1T6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044605.1">
        <property type="protein sequence ID" value="ENSBIXP00000033151.1"/>
        <property type="gene ID" value="ENSBIXG00000015873.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011208.1">
        <property type="protein sequence ID" value="ENSBIXP00005029302.1"/>
        <property type="gene ID" value="ENSBIXG00005009218.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875664"/>
    <dbReference type="CTD" id="27033"/>
    <dbReference type="GeneTree" id="ENSGT00940000162716"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000553">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001279">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="16">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="310"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="489" mass="53684" checksum="79647318773B2743" modified="2019-09-18" version="1">MPLSPTRLPSPYASDRLVRLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGRWAVVKGISPSTFAQLLHFVYGESLELHPGELGPLEEAARTLGVQSLEEACFRARRDMAREELGPGLKKQEELEKPTRDSMRVVGAFGEKQRPEKFVRAGGKEQETLHRPRPPRESPDTAEAILEGRGEQMRPKEQLHQALVGHGRVGGKQEVVMWLRDSARGSEESLREFPGPLPPAGSFQTGITPSPWWAEAPWLGEGQSALWSILLLPPRYGTPFSHSIPMTGAWQEVWPPDQRIPLSLNLHKGLWSQNQLASSSPTPGSLPQVPTQLSPGETEESDQRRTGELATCVGQVGTASHPSLPHPPPPTPARSRPYSCSVCGKRFSLKHQMETHYRVHTGEKPFSCSLCPQRSRDFSAMTKHLRTHGAAPYRCPLCQAGCPSLASMQAHMRGHSPSQLPPGWTIRSTFLYSSSSRPSRASTSPSRPSSSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2L8</accession>
    <name>A0A4W2E2L8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">CAMK1D</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027416033.1">
        <property type="nucleotide sequence ID" value="XM_027560232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E2L8"/>
    <dbReference type="SMR" id="A0A4W2E2L8"/>
    <dbReference type="STRING" id="30522.A0A4W2E2L8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044830.1">
        <property type="protein sequence ID" value="ENSBIXP00000033453.1"/>
        <property type="gene ID" value="ENSBIXG00000016270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903682"/>
    <dbReference type="CTD" id="57118"/>
    <dbReference type="OMA" id="RPKVQPC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14168">
        <property type="entry name" value="STKc_CaMKI_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000203">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="23"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="385" mass="42893" checksum="F22D8FF0196B19C7" modified="2019-09-18" version="1">MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDSKLFEQILKAEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIAGDTALSKNIHESVSAQIRKNFAKSKWRQAFNATAVVRHMRKLHLGSSLDSSNASVSSSLSLASQKDCLTSSTLCSFVSSASGVSGVGADRRPRPTTVTTVHSGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E399</accession>
    <name>A0A4W2E399_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E399"/>
    <dbReference type="STRING" id="30522.A0A4W2E399"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019681.1">
        <property type="protein sequence ID" value="ENSBIXP00000033361.1"/>
        <property type="gene ID" value="ENSBIXG00000016150.1"/>
    </dbReference>
    <dbReference type="OMA" id="THTELSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="423"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="861" mass="100754" checksum="E44F977C24448177" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5H3</accession>
    <name>A0A4W2E5H3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Aggrecan core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Cartilage-specific proteoglycan core protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">ACAN</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms a complex (via covalent bonds) with MATN1; the interaction increases with age of the organism via an increase in occupancy of MATN1 binding sites. Interacts with FBLN1. Interacts with COMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aggrecan/versican proteoglycan family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2E5H3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043722.1">
        <property type="protein sequence ID" value="ENSBIXP00000031999.1"/>
        <property type="gene ID" value="ENSBIXG00000014439.1"/>
    </dbReference>
    <dbReference type="OMA" id="EDWIVTQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03588">
        <property type="entry name" value="CLECT_CSPGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05900">
        <property type="entry name" value="Ig_Aggrecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03520">
        <property type="entry name" value="Link_domain_CSPGs_modules_2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000451">
        <property type="entry name" value="aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000009">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033987">
        <property type="entry name" value="CSPG_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF42">
        <property type="entry name" value="AGGRECAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
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    <sequence length="2366" mass="246739" checksum="7E3C85D6D3F6057A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6U2</accession>
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            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <sequence length="293" mass="32544" checksum="1205498ACD450963" modified="2019-09-18" version="1">IYMKEMQTRNQTEVTAFILLGLSDNSELQVILFGLFLLIYMATVVGNLGVIVLIKMDPCLHTPMYFFLSSLSFVDASYSSSVTPKMLVNLVAESKAISFNGRAAQFYFFGSFLGTECLLLATMAYDLYIAIWNPLLYPVLMSGRICFLLAATSFLADFGNVAIHTGMTFKLSFCGSDKTNHFYCDTPALLKLSCSDTRINGIVIMAFSSVTVTGCVMIVLTSYLCILVAILRMPSLEGRHRAFSTCTSYSMEQDKIVSVFYTVVITMLNPLIYSLKNKDVKGALKKILQQQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E748</accession>
    <name>A0A4W2E748_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3">Glycine N-acyltransferase-like protein</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E748"/>
    <dbReference type="STRING" id="30522.A0A4W2E748"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045800.1">
        <property type="protein sequence ID" value="ENSBIXP00000034815.1"/>
        <property type="gene ID" value="ENSBIXG00000017905.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047961">
        <property type="term" value="F:glycine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010313">
        <property type="entry name" value="Glycine_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013652">
        <property type="entry name" value="Glycine_N-acyltransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015938">
        <property type="entry name" value="Glycine_N-acyltransferase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298:SF4">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298">
        <property type="entry name" value="L-COA N-ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08444">
        <property type="entry name" value="Gly_acyl_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06021">
        <property type="entry name" value="Gly_acyl_tr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycine N-acyltransferase N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine N-acyltransferase C-terminal" evidence="5">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="224" mass="25936" checksum="E0E89A90F3086217" modified="2019-09-18" version="1">MLILQMRKRSLRKETFQNIFLNVLHARLVNEHWELGKNEKSLKYVECCLQNFEGFGVLSSEGKSISWFLTEQSCEIRMGYTFPKYRGQGHMRQMGCHSIAFFFFFKKKKKKYRFISMWQKIKKKLNRYCSVLGSKMFFVAGISGNAPQRNIVDGTIVHFKCFLSGEKKNVSANECGSLRATKPSAYLWLRRCSGHTAVIVLSSFSCILQNIRSLAPCCHAFPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E789</accession>
    <name>A0A4W2E789_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cysteine rich secretory protein LCCL domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CRISPLD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027417021.1">
        <property type="nucleotide sequence ID" value="XM_027561220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E789"/>
    <dbReference type="SMR" id="A0A4W2E789"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024084.1">
        <property type="protein sequence ID" value="ENSBIXP00000035263.1"/>
        <property type="gene ID" value="ENSBIXG00000018500.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024194.1">
        <property type="protein sequence ID" value="ENSBIXP00005036512.1"/>
        <property type="gene ID" value="ENSBIXG00005002938.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904276"/>
    <dbReference type="CTD" id="83690"/>
    <dbReference type="GeneTree" id="ENSGT00940000156473"/>
    <dbReference type="OMA" id="MVWDVEL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060325">
        <property type="term" value="P:face morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061484">
        <property type="term" value="P:hematopoietic stem cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18813">
        <property type="entry name" value="CAP_CRISPLD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.130.20:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain-containing 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
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        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF9">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN LCCL DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LCCL" evidence="6">
        <location>
            <begin position="289"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="6">
        <location>
            <begin position="390"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="500" mass="56697" checksum="D9FEEB4357818924" modified="2019-09-18" version="1">MKCTVREWFRVTTVLLMAPAIPAMVVPNATLLEKLLEKYMDEDGVWWIAKQRGKRAITDNDMQSILDLHNKLRSQVYPTASNMEYMTWDVELERSAESWAETCLWEHGPANLLPSIGQNLGAHWGRYRPPTFHVQAWYDEVRDFSYPYEHECNPYCPFRCSGPVCTHYTQVVWATSSRIGCAINLCHNMNIWGQIWPKAVYLVCNYSPKGNWWGHAPYKHGRPCSACPPSFGGGCRENLCYKEGSDSYYPAQEEETNEIERQQSQVHDTHVRTVADDSNRNEVISTQQMSQIVSCEVRLRDQCKGTTCNRYECPAGCLDSKAKVIGSVHYEMQSSICRAAIHYGIIDNDGGWVDITRQGRKHYFIKSNRNGVQTIGKYQSANSFTVSKVTVQAVTCETTVEQLCPFHKPASHCPRVYCPRNCMQANPHYARVIGTRIYSDLSSICRAAVHAGVVQNQGGYVDVMPVDKRKTYTASFQNGIFSESLQNPPGGKAFRVFAVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAL7</accession>
    <name>A0A4W2EAL7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC113902906</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase CarF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026067.1">
        <property type="protein sequence ID" value="ENSBIXP00000036120.1"/>
        <property type="gene ID" value="ENSBIXG00000019515.1"/>
    </dbReference>
    <dbReference type="OMA" id="CITNGWL"/>
    <dbReference type="UniPathway" id="UPA00199"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050207">
        <property type="term" value="F:plasmanylethanolamine desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019547">
        <property type="entry name" value="Lipid_desat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052601">
        <property type="entry name" value="Plasmalogen_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48177:SF1">
        <property type="entry name" value="PLASMANYLETHANOLAMINE DESATURASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48177">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10520">
        <property type="entry name" value="Lipid_desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Lipid desaturase" evidence="6">
        <location>
            <begin position="86"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="271" mass="31338" checksum="46B4FBFE87D60CB6" modified="2019-09-18" version="1">MAGAEDGPGQQPELEDDEAASCRRWGAQHAGARELAALYSPGKRFQEWCCVVLCFSLIAHNMAHLLLLARWEHTPLVMLGMVAGALLADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLLTLLPLLNMAYKFRTQSPEVLEQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVVFLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLERMGFWRRLEDIIQALTGEKPRADDMKWAQKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAU3</accession>
    <name>A0A4W2EAU3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026272.1">
        <property type="protein sequence ID" value="ENSBIXP00000036209.1"/>
        <property type="gene ID" value="ENSBIXG00000003511.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="193"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="377"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="649" mass="75057" checksum="5894EEE47F9BC874" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB59</accession>
    <name>A0A4W2EB59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EB59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021470.1">
        <property type="protein sequence ID" value="ENSBIXP00000034121.1"/>
        <property type="gene ID" value="ENSBIXG00000017063.1"/>
    </dbReference>
    <dbReference type="OMA" id="DYHCSKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001530">
        <property type="entry name" value="Zinc finger protein 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000269">
        <property type="entry name" value="Zinc finger protein 287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000238">
        <property type="entry name" value="Zinc finger protein 485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000102">
        <property type="entry name" value="zinc finger protein 850 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="442" mass="51401" checksum="B985A0A34BAE9D06" modified="2019-09-18" version="1">MLSNHLILYISPIHIIKKLQSKENTNSTDTLQTVILGRHESHGVQDFYLREIRGNTLEFECQWKDDERNYKGVLVIHNEKLTDRKDQRGRSNAGNNAIENKLALSFQDELQIFRMERKIFECNQVENVINNSSSISALQRIPPSVQTNTSNMYGNNFIHSSILTKDHKAHREKPYRCNDCDKTFTHVSHLTKHQVVHTGERPYKCDTCGKDFSQSSNLATHRRIHTGEKPYKCNECGKDFNRKSNLETHQRIHTGEKPYKCNVCGKAFRVCSSLRSHQVIHTGEKDYHCSKCNKAFSRNSKLLKHQRIHTRGQPYKCDACDKAFILRSSLINHQVVHTRGKSYPCNECGKMFIKRSHLRLHERIHTGEKPYKCTECGKGFRQWSDIRIHLRIHAGEKPFKCSKCGKSFTRSSHLTRHQIIHTGEKPYKCEVCDNFKSLLTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBD2</accession>
    <name>A0A4W2EBD2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM171 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBD2"/>
    <dbReference type="STRING" id="30522.A0A4W2EBD2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019431.1">
        <property type="protein sequence ID" value="ENSBIXP00000033259.1"/>
        <property type="gene ID" value="ENSBIXG00000016013.1"/>
    </dbReference>
    <dbReference type="OMA" id="HLICAGP"/>
    <dbReference type="Proteomes" id="UP000314981">
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        <source>
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        </source>
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    <sequence length="794" mass="90700" checksum="C22EFBB9F46896A9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDM5</accession>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <end position="36"/>
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        <location>
            <begin position="87"/>
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        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="188" mass="20608" checksum="1D7C3C7802B00908" modified="2019-09-18" version="1">MKMTVLKSKRRSQLLFLVTMTLTITTICLLFFALLWKAGNLVDLPNLRVGFYNFCLWHEGTGALQCYQFPELEALGVPRVGLALARLGVYGALVLTLFVPLPFLLAWCNNNEGEWQLAVGFLATSSVLLASGLGLFLTYTWKWLQLSLLGPGSLALGAAQTLLILLLVATAVFPQRAKDRSWGATSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EG49</accession>
    <name>A0A4W2EG49_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Testis-specific Y-encoded-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TSPYL5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. Involved in regulation of p53/TP53. Suppresses p53/TP53 protein levels and promotes its ubiquitination; the function is dependent on USP7 and independent on MDM2. Proposed to displace p53/TP53 from interaction with USP7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with USP7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027561483.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027561484.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027417286.1">
        <property type="nucleotide sequence ID" value="XM_027561485.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2EG49"/>
    <dbReference type="STRING" id="30522.A0A4W2EG49"/>
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        <property type="protein sequence ID" value="ENSBIXP00000036281.1"/>
        <property type="gene ID" value="ENSBIXG00000003538.1"/>
    </dbReference>
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    <dbReference type="OMA" id="APETCST"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Testis-specific Y-encoded-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000008">
        <property type="entry name" value="Testis-specific Y-encoded-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="413" mass="44539" checksum="2F3097E0B00BAFAF" modified="2019-09-18" version="1">MSGRSKGRRSSRAKGRGKSRAKGRVRAAPDDAPRDPDPPECQRLGEETKAAQVQAGAVWGGPEGAAPAPPRRPGEEAACRLPLDCGLALRARAAGTRGQAVTRPCLVKATSFPERLVSDTVFVGTVGTVARPRNGPRVGSRRGPAAKKAPDTCSAVGRGSAALAGGKPKKGPAAEAASVPVGEEKVENAGSGPPATEGSMDTLENVQLKLETMNAQADRAYLRLSRKFGQLRLHHLERRNLLIQNIPGFWGQAFQNHPQLSSFLNNQDKEVLSFLNSLEVEELGLARLGYKIKFYFGRNPYFQNKVLIKEYGCGPSGQVVSRSTPIQWLPGHDLQTLSQGNPDNSRSFFGWFSNHSSIESDKIVEIINEELWPNPLQYYLMSEGARAEKGKEGRPGPARPPGETPEPGVNKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EG65</accession>
    <name>A0A4W2EG65_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EG65"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002074.1">
        <property type="protein sequence ID" value="ENSBIXP00000031121.1"/>
        <property type="gene ID" value="ENSBIXG00000013290.1"/>
    </dbReference>
    <dbReference type="OMA" id="RKMSHLY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038935">
        <property type="entry name" value="C5orf52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35666">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 4921536K21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35666:SF1">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 4921536K21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17666">
        <property type="entry name" value="DUF5528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="181" mass="20286" checksum="5CE46B2A94026D6A" modified="2019-09-18" version="1">SGETPASFSPSWSSGDENSAVGQQLRPSVTWNLDSPVAGAVAPQATSSSGTYPASAFFRHSRLSNRRDTHVGSQPKICFLRPRSAQPLVLFSLMNSSEAAVKKFLPKSHLSRVIIRDNLSAQRIYEMEVKAADKTKKKMNHLYDHLKKKFMTDQLRKLGRWRRESTNIRQYLDSSSEGTCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJ90</accession>
    <name>A0A4W2EJ90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ADP-ribosylation factor 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with ARL14EP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJ90"/>
    <dbReference type="SMR" id="A0A4W2EJ90"/>
    <dbReference type="STRING" id="30522.A0A4W2EJ90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028862.1">
        <property type="protein sequence ID" value="ENSBIXP00000037331.1"/>
        <property type="gene ID" value="ENSBIXG00000003932.1"/>
    </dbReference>
    <dbReference type="OMA" id="GGQISKM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="148" mass="17073" checksum="9DA7530BA7758F91" modified="2019-09-18" version="1">MGKVLSKIFGNKEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJP3</accession>
    <name>A0A4W2EJP3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin--tyrosine ligase</fullName>
            <ecNumber evidence="11">6.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TTL</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + L-tyrosine + ATP = C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-L-tyrosyl-[tubulin] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17605"/>
            <dbReference type="Rhea" id="RHEA-COMP:16434"/>
            <dbReference type="Rhea" id="RHEA-COMP:16435"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:149554"/>
            <dbReference type="ChEBI" id="CHEBI:149555"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.25" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJP3"/>
    <dbReference type="SMR" id="A0A4W2EJP3"/>
    <dbReference type="STRING" id="30522.A0A4W2EJP3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020126.1">
        <property type="protein sequence ID" value="ENSBIXP00000033564.1"/>
        <property type="gene ID" value="ENSBIXG00000016381.1"/>
    </dbReference>
    <dbReference type="OMA" id="LDKTCHL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004835">
        <property type="term" value="F:tubulin-tyrosine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000049">
        <property type="entry name" value="tubulin--tyrosine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11480:FF:000001">
        <property type="entry name" value="tubulin--tyrosine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052492">
        <property type="entry name" value="Tubulin-tyrosine_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46570">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46570:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="377" mass="43270" checksum="29DE18B6A3997CEC" modified="2019-09-18" version="1">MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLMQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIHPPLHSSRTDEREFFLASYNRKKEEGEGNVWIAKSSAGAKGEGILISSDATELLDFIDNQGQVHVIQKYLERPLLLEPGHRKFDIRSWVLVDHQFNIYLYREGVLRTASEPYHMDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFEAFNRYLTSALNITLESSILLQIKHIIRSCLMSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAIASVFPPPDAEQQPPQPATFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKH1</accession>
    <name>A0A4W2EKH1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKH1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023116.1">
        <property type="protein sequence ID" value="ENSBIXP00000034844.1"/>
        <property type="gene ID" value="ENSBIXG00000017935.1"/>
    </dbReference>
    <dbReference type="OMA" id="REAWHVV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="407" mass="47061" checksum="D7AFD9A18205DB40" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANICWIMEKAREFQKNIYFCFIDYAKAFDSVDHNKLWKILEEMGIPDHLNCFLRNLYAGQEAIVRTGHGTTDWFQIGRVRQGCILSPCLFNSYAEYIMRNAGLEEAQTGIRIARRNINNLRYADDTTLMAESEEELKSLLMKVKEESENIGLKLNIQKTKIMASGPITSWEIDGETVETVLDFIFLGSKITADGDCSHEIKRCLLLRRKVMTNLDSILKSRDITLPTKVRLVKAMVFPVVIYGCESWTVKKAECQRIDTFELWCWRRLLRVPWTARRSNQSILKEVSPGYSLEGLMLKLKLQYFGHLMQRVDSLEKTLMLGGIGGRRRRGRQRMRWQDGITDSKDMGLGELQGMVMDREAWHVVIHGVTKSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKS5</accession>
    <name>A0A4W2EKS5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 704</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027417054.1">
        <property type="nucleotide sequence ID" value="XM_027561253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKS5"/>
    <dbReference type="STRING" id="30522.A0A4W2EKS5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024336.1">
        <property type="protein sequence ID" value="ENSBIXP00000035380.1"/>
        <property type="gene ID" value="ENSBIXG00000018634.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904296"/>
    <dbReference type="CTD" id="619279"/>
    <dbReference type="OMA" id="QKVEGSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052253">
        <property type="entry name" value="CR1/CR2-DNA-binding_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006">
        <property type="entry name" value="PAPILLOMAVIRUS REGULATORY FACTOR PRF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006:SF7">
        <property type="entry name" value="ZINC FINGER PROTEIN 704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="362"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="267"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="516"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="582" mass="62781" checksum="0358D461A8A07171" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKY6</accession>
    <name>A0A4W2EKY6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">tRNA (uracil-5-)-methyltransferase homolog A</fullName>
            <ecNumber evidence="11">2.1.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">mRNA (uracil-5-)-methyltransferase TRMT2A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs. Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs. Also able to mediate the formation of 5-methyl-uridine in some mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a uridine in mRNA + S-adenosyl-L-methionine = a 5-methyluridine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69863"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:69864"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:42713"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKY6"/>
    <dbReference type="STRING" id="30522.A0A4W2EKY6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025056.1">
        <property type="protein sequence ID" value="ENSBIXP00000035691.1"/>
        <property type="gene ID" value="ENSBIXG00000007475.1"/>
    </dbReference>
    <dbReference type="OMA" id="TPLWNMP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12439">
        <property type="entry name" value="RRM_TRMT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1070:FF:000005">
        <property type="entry name" value="tRNA (Uracil-5-)-methyltransferase homolog A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045850">
        <property type="entry name" value="TRM2_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034262">
        <property type="entry name" value="TRMT2A_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904:SF2">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 17" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 17" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="584"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="612" mass="66883" checksum="B63B812E036BD3A3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELC6</accession>
    <name>A0A4W2ELC6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Telomere length and silencing protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">C11H9orf78</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELC6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026735.1">
        <property type="protein sequence ID" value="ENSBIXP00000036411.1"/>
        <property type="gene ID" value="ENSBIXG00000019887.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="232"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33593" checksum="BEED0F48BC188228" modified="2019-09-18" version="1">MPVTGKTFRRRRADSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELY5</accession>
    <name>A0A4W2ELY5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transforming growth factor beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TGFB2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with Transforming growth factor beta-2 (TGF-beta-2) chain; interaction is non-covalent and maintains (TGF-beta-2) in a latent state. Interacts with LRRC32/GARP; leading to regulate activation of TGF-beta-2. Interacts with NREP; the interaction results in a decrease in TGFB2 autoinduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Interacts with MFAP5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Transforming growth factor beta-2: Homodimer; disulfide-linked. Transforming growth factor beta-2: Interacts with TGF-beta receptors (TGFBR1 and TGFBR2), leading to signal transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027420258.1">
        <property type="nucleotide sequence ID" value="XM_027564457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELY5"/>
    <dbReference type="SMR" id="A0A4W2ELY5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029334.1">
        <property type="protein sequence ID" value="ENSBIXP00000037524.1"/>
        <property type="gene ID" value="ENSBIXG00000021106.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055132.1">
        <property type="protein sequence ID" value="ENSBIXP00005027383.1"/>
        <property type="gene ID" value="ENSBIXG00005030716.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906403"/>
    <dbReference type="CTD" id="7042"/>
    <dbReference type="GeneTree" id="ENSGT00940000157390"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005160">
        <property type="term" value="F:transforming growth factor beta receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19385">
        <property type="entry name" value="TGF_beta_TGFB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000004">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000002">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016319">
        <property type="entry name" value="TGF-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003940">
        <property type="entry name" value="TGFb2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF141">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA-2 PROPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001787">
        <property type="entry name" value="TGF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01423">
        <property type="entry name" value="TGFBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01425">
        <property type="entry name" value="TGFBETA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 17 19" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10 17" id="KW-0497">Mitogen</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044535894" description="Transforming growth factor beta" evidence="17">
        <location>
            <begin position="21"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="20">
        <location>
            <begin position="299"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="317"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="346"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="350"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="18">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR001787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001787-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="414" mass="47692" checksum="98C76C454EC4D6C9" modified="2019-09-18" version="1">MHYCVLSAFLLLHLVTVALSLSTCSTLDMDQFMRKRIEAIRGQILSKLKLTSPPEDYPEPEEVPPEVISIYNSTRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPSFLPSENAIPPTFYRPYFRIVRFDVSSMEKNASNLVKAEFRVFRLQNPKARVPEQRIELYQILKSKDLTSPTQRYIDSKVVKTRAEGEWLSFDVTDAVHEWLHHKDRNLGFKISLHCPCCTFVPSNNYIIPNKSEELEARFAGIDGTSTYTSGDQKTIKSTRKKNSGKSPHLLLMLLPSYRLESQQSNRRKKRALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASASPCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EML1</accession>
    <name>A0A4W2EML1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EML1"/>
    <dbReference type="STRING" id="30522.A0A4W2EML1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030507.1">
        <property type="protein sequence ID" value="ENSBIXP00000038023.1"/>
        <property type="gene ID" value="ENSBIXG00000021744.1"/>
    </dbReference>
    <dbReference type="OMA" id="QLWVIAK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015643">
        <property type="term" value="F:toxic substance binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040416">
        <property type="entry name" value="TMEM181"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054077">
        <property type="entry name" value="TMEM181_GOLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31918">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31918:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21885">
        <property type="entry name" value="TMEM181_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM181 GOLD" evidence="7">
        <location>
            <begin position="75"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="6">
        <location>
            <begin position="186"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="476" mass="55720" checksum="2AE91F34590718C9" modified="2019-09-18" version="1">MDAEYPAFEPPLCSELKHLCKRLQEAYRELKEDLTPFRDDRYYRLAPMRLYTLSKHHFVLVFVVFFVCFGLTVFPIQIFSKPLSTYNQQLWLTCVVELDQSKETSIQTTFTMTVKVDGVAQDGTTKFIHNKVHNRTRTLTCAEKCAEIIVAHLSYLNYTKYTVTVGFEHLKRPIKEMNFTWKTYDPAFSHLEIWFRFVFVVLTFIVTCLFAHSLRKFSMRDWGIEQKWMSILLPLLLLYNDPFFPLSFLVNSWFPGMLDDLFQSVFLCALLLFWLCVYHGVRVQGERKCLTFYLPKFFIVGLLWLASVTLGIWQTERLPLRPHGRSRACLVSCQGVKVFFMVVAVVYILYLLFLIVRACSELGHMPYVDLRLKFLTALTFVVLVISIVILYLRFGAQVLQDNFVAELSTHYQNYILFWEDVRHTNALYESQLKDNPAFSMLNDSDDDVIYGSDYEEMPLQNGQAIRAQYKEGSESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EP48</accession>
    <name>A0A4W2EP48_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GALNT6</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EP48"/>
    <dbReference type="SMR" id="A0A4W2EP48"/>
    <dbReference type="STRING" id="30522.A0A4W2EP48"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048531.1">
        <property type="protein sequence ID" value="ENSBIXP00000038518.1"/>
        <property type="gene ID" value="ENSBIXG00000002829.1"/>
    </dbReference>
    <dbReference type="OMA" id="SWFLHNI"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000024">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000039">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF58">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="22">
        <location>
            <begin position="494"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="622" mass="71138" checksum="05010D38D433A376" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPU1</accession>
    <name>A0A4W2EPU1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPU1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032571.1">
        <property type="protein sequence ID" value="ENSBIXP00000038905.1"/>
        <property type="gene ID" value="ENSBIXG00000004453.1"/>
    </dbReference>
    <dbReference type="OMA" id="VVEIFCN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="94" mass="10594" checksum="B2D094690BDA7588" modified="2019-09-18" version="1">KEQVLQPTTVVSLEGAVAEISCNHSVSDVYDFFWYLHFPGFAPRLLIKGSKPSQQGRYNMTYERFSSSLLILQVQTADAGVYYCALRYTEAGHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQU0</accession>
    <name>A0A4W2EQU0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GTP-binding protein REM 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Rad and Gem-like GTP-binding protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">REM2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Binds GTP saturably and exhibits a low intrinsic rate of GTP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027409131.1">
        <property type="nucleotide sequence ID" value="XM_027553330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EQU0"/>
    <dbReference type="SMR" id="A0A4W2EQU0"/>
    <dbReference type="STRING" id="30522.A0A4W2EQU0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049041.1">
        <property type="protein sequence ID" value="ENSBIXP00000039251.1"/>
        <property type="gene ID" value="ENSBIXG00000023208.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035503.1">
        <property type="protein sequence ID" value="ENSBIXP00005021573.1"/>
        <property type="gene ID" value="ENSBIXG00005024566.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899935"/>
    <dbReference type="CTD" id="161253"/>
    <dbReference type="GeneTree" id="ENSGT00940000160062"/>
    <dbReference type="OMA" id="DWPPQAL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04148">
        <property type="entry name" value="RGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001032">
        <property type="entry name" value="GTP-binding protein REM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF5">
        <property type="entry name" value="GTP-BINDING PROTEIN REM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="340" mass="37269" checksum="E8AAE080205517DE" modified="2019-09-18" version="1">MHTDFDTDMDTDTETTALCPSSSHQASPPETPTPEADATLLKKPEKLLAGLDRGRPPPALGAPRRRGSMPVPYKHQLRRAQAVDELDWPPQASSSGSSDSLGSGEAAPTQKDGIFKVMLVGESGVGKSTLAGTFGGLQGDSAHELENPEDTYERRIMVDKEEVTLVVYDIWEQGDAGGWLRDHCLQTGDAFLIVFSVTDRRSFSKVPETLLRLRAGRPHHDLPVILVGNKSDLARSREVSLEEGRHLAGTLSCKHIETSAALHHNTRELFEGAVRQIRLRRGRNGAGGPRPEWGSPEGLAPPARRESLTKKAKRFLANLVPRNAKFFKQRSRSCHDLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVE4</accession>
    <name>A0A4W2EVE4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LysM and putative peptidoglycan-binding domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LYSMD1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027389764.1">
        <property type="nucleotide sequence ID" value="XM_027533963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EVE4"/>
    <dbReference type="SMR" id="A0A4W2EVE4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036851.1">
        <property type="protein sequence ID" value="ENSBIXP00000040792.1"/>
        <property type="gene ID" value="ENSBIXG00000024935.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041036.1">
        <property type="protein sequence ID" value="ENSBIXP00005025350.1"/>
        <property type="gene ID" value="ENSBIXG00005027861.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887102"/>
    <dbReference type="CTD" id="388695"/>
    <dbReference type="GeneTree" id="ENSGT00940000160002"/>
    <dbReference type="OMA" id="EVWPHSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.350.10:FF:000010">
        <property type="entry name" value="LysM and putative peptidoglycan-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF2">
        <property type="entry name" value="AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="4">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="225" mass="24728" checksum="19F5E6A292860AE3" modified="2019-09-18" version="1">MASPSRQAPLGGSGLLHGSRARSYGSLVQSACSPVRERRLEHQLAPGDTLAGLALKYGVTMEQIKRANRLYTNDSIFLKKTLYIPILTEPRDLFNGLDSEEEKDGEEAVQPSKDEVRPHSAERKKRERGLGHANGEPLPTAGQEPARHDLSASDFLKKLDSQISLSKKAAAQKLKKGESGIPGEDSSLHLSSPRMQQRAVLGPVPLTQTSRTRTLRDQEDEIFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZM9</accession>
    <name>A0A4W2EZM9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZM9"/>
    <dbReference type="SMR" id="A0A4W2EZM9"/>
    <dbReference type="STRING" id="30522.A0A4W2EZM9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040442.1">
        <property type="protein sequence ID" value="ENSBIXP00000042540.1"/>
        <property type="gene ID" value="ENSBIXG00000026544.1"/>
    </dbReference>
    <dbReference type="OMA" id="INKLARN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="133" mass="15413" checksum="FCBE664E39CAA64E" modified="2019-09-18" version="1">MAATMKKTAAEDVNITFEDQQKIKKFAQNTSRITELKEEIEVKKKQLQNLEDVCEDIMLADDNFLMTPYHIGDVFINHSQEETQEMLEEAKKNLQEETDALESRMESIQWVLADLKLHAKSGSNITLKLKHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F068</accession>
    <name>A0A4W2F068_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PPFIA binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PPFIBP1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-beta subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397396.1">
        <property type="nucleotide sequence ID" value="XM_027541595.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397405.1">
        <property type="nucleotide sequence ID" value="XM_027541604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F068"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040264.1">
        <property type="protein sequence ID" value="ENSBIXP00000042425.1"/>
        <property type="gene ID" value="ENSBIXG00000026477.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892589"/>
    <dbReference type="CTD" id="8496"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09563">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09566">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09569">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000005">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000007">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000017">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058914">
        <property type="entry name" value="LIPB1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037617">
        <property type="entry name" value="LIPB1/2_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037618">
        <property type="entry name" value="LIPB1/2_SAM_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037619">
        <property type="entry name" value="LIPB1/2_SAM_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF16">
        <property type="entry name" value="LIPRIN-BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26022">
        <property type="entry name" value="CC_Liprin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144266">
        <property type="entry name" value="MPN010-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="638"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="710"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="402"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="99"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="554"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="575"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1002" mass="112524" checksum="1B9A7DE4011A88A2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FF90</accession>
    <name>A0A4W2FF90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase 17B</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DAP kinase-related apoptosis-inducing protein kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">STK17B</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with CHP1; the interaction induces CHP1 to translocate from the Golgi to the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum-Golgi intermediate compartment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027419135.1">
        <property type="nucleotide sequence ID" value="XM_027563334.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027419143.1">
        <property type="nucleotide sequence ID" value="XM_027563342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FF90"/>
    <dbReference type="SMR" id="A0A4W2FF90"/>
    <dbReference type="STRING" id="30522.A0A4W2FF90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042958.1">
        <property type="protein sequence ID" value="ENSBIXP00000043915.1"/>
        <property type="gene ID" value="ENSBIXG00000028091.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009019.1">
        <property type="protein sequence ID" value="ENSBIXP00005004091.1"/>
        <property type="gene ID" value="ENSBIXG00005010089.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905690"/>
    <dbReference type="CTD" id="9262"/>
    <dbReference type="GeneTree" id="ENSGT00940000154014"/>
    <dbReference type="OMA" id="LSHPWLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0090543">
        <property type="term" value="C:Flemming body"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="term" value="P:positive regulation of fibroblast apoptotic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14198">
        <property type="entry name" value="STKc_DRAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein kinase 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042763">
        <property type="entry name" value="ST17B_STKc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12 21 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 21 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="33"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="307"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060827"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00062765"/>
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            <dbReference type="ARBA" id="ARBA00069224"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="372" mass="42449" checksum="515865CF84E983CF" modified="2019-09-18" version="1">MLRRRFDCRSISGMLTTTPQTPIKVENFNNFYTLTSKELGRGKFAVVRQCISKSTGQEYAAKFLKKRRRGQDCRAEILHEIAVLELTKSCPHVINLHEVYENTSEIILILEYAAGGEIFNLCLPELAEMVSENDIIRLIKQILEGVHYLHQNNIVHLDLKPQNILLSSIYPLGDIKIVDFGMSRKIGNACELREIMGTPEYLAPEILNYDPITTATDMWNVGVIAYMLLTHTSPFVGEDNQETYLNISQVNVDYSEETFSSVSQLATDFIQRLLVKNPEKRPTAEICLSHSWLQQWDFGNLFHPEETSSFSQSQDHPVRSSEDRTHRPSCNGTCSDREDKENIPEDSSTVSKRFRFDDSLPSPHELVSDLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FFU0</accession>
    <name>A0A4W2FFU0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TIMM9</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer; composed of 3 copies of TIMM9 and 3 copies of TIMM10/TIM10A, named soluble 70 kDa complex. The complex forms a 6-bladed alpha-propeller structure and associates with the TIMM22 component of the TIM22 complex. Interacts with multi-pass transmembrane proteins in transit. Also forms a complex composed of TIMM9, TIMM10/TIM10A and FXC1/TIM10B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410013.1">
        <property type="nucleotide sequence ID" value="XM_027554212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FFU0"/>
    <dbReference type="SMR" id="A0A4W2FFU0"/>
    <dbReference type="STRING" id="30522.A0A4W2FFU0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049890.1">
        <property type="protein sequence ID" value="ENSBIXP00000021279.1"/>
        <property type="gene ID" value="ENSBIXG00000004922.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008610.1">
        <property type="protein sequence ID" value="ENSBIXP00005004290.1"/>
        <property type="gene ID" value="ENSBIXG00005010269.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900435"/>
    <dbReference type="CTD" id="26520"/>
    <dbReference type="GeneTree" id="ENSGT00940000160102"/>
    <dbReference type="OMA" id="QDFLRMY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000004">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="16">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10378" checksum="00F17CF6332ABF48" modified="2019-09-18" version="1">MAAQIPESDQIKQFKEFLGTYNKLTETCFLDCVKDFTTREVKPEETTCSEHCLQKYLKMTQRISMRFQEYHIQQNEALAAKAGLLGQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G199</accession>
    <name>A0A4W2G199_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 22">Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1</fullName>
            <ecNumber evidence="5 22">2.4.1.122</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">C1GALT1</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP-alpha-D-galactose = a 3-O-[beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56200"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="Rhea" id="RHEA-COMP:13922"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:137949"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:56201"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP-alpha-D-galactose = a 3-O-[beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56196"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13923"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="ChEBI" id="CHEBI:137950"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:56197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>an N-acetyl-alpha-D-galactosaminyl derivative + UDP-alpha-D-galactose = a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28257"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="EC" id="2.4.1.122"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; disulfide-linked. Interacts with the C1GALT1C1 chaperone; required for galactosyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 22">Membrane</location>
            <topology evidence="2 22">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.122" evidence="5 22"/>
    <dbReference type="RefSeq" id="XP_027395889.1">
        <property type="nucleotide sequence ID" value="XM_027540088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G199"/>
    <dbReference type="SMR" id="A0A4W2G199"/>
    <dbReference type="STRING" id="30522.A0A4W2G199"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050656.1">
        <property type="protein sequence ID" value="ENSBIXP00000041329.1"/>
        <property type="gene ID" value="ENSBIXG00000005226.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021329.1">
        <property type="protein sequence ID" value="ENSBIXP00005012129.1"/>
        <property type="gene ID" value="ENSBIXG00005016418.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891700"/>
    <dbReference type="CTD" id="56913"/>
    <dbReference type="GeneTree" id="ENSGT00940000155000"/>
    <dbReference type="OMA" id="WLLSKHD"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060576">
        <property type="term" value="P:intestinal epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001822">
        <property type="term" value="P:kidney development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000007">
        <property type="entry name" value="Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF13">
        <property type="entry name" value="GLYCOPROTEIN-N-ACETYLGALACTOSAMINE 3-BETA-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 22" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15 22" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 22" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="23">
        <location>
            <begin position="89"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00064802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU364049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="368" mass="43041" checksum="DF82C20C98CCF250" modified="2019-09-18" version="1">MASKSWLNILTFLCGSAVGFVLCSQLLSILFEEQKDIQPSILHNDPHARHSDDNEQNHLEGQMNFDADASQHKDENTDIADKLYQKVKILCWVMTGPQNLEKKAKHVKATWAQRCNKVLFMSSEENKDFPAVGLKTREGRDQLYWKTIKAFQYVHDHYLEDADWFMKADDDTYVILDNLRWLLSKYNPEEPIYFGRRFKPYVKQGYMSGGAGYVLSKEALKRFVEAFKTDKCTHSSSIEDLALGRCMEIINVEAGDSRDTTGKETFHPFVPEHHLIKGYLPRTFWYWNYNYYPPVEGPGCCSDLAVSFHYVDPTTMYELEYLVYHLRPYGYLYRYQPALPEKMLKEVSQIYKNEDTNVNQEPLKEKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G421</accession>
    <name>A0A4W2G421_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mal, T cell differentiation protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MAL2</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027417063.1">
        <property type="nucleotide sequence ID" value="XM_027561262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G421"/>
    <dbReference type="SMR" id="A0A4W2G421"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024381.1">
        <property type="protein sequence ID" value="ENSBIXP00000013803.1"/>
        <property type="gene ID" value="ENSBIXG00000018673.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046221.1">
        <property type="protein sequence ID" value="ENSBIXP00005012550.1"/>
        <property type="gene ID" value="ENSBIXG00005016758.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904305"/>
    <dbReference type="CTD" id="114569"/>
    <dbReference type="GeneTree" id="ENSGT00940000159514"/>
    <dbReference type="OMA" id="TTVCYGC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013295">
        <property type="entry name" value="MAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF42">
        <property type="entry name" value="PROTEIN MAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01884">
        <property type="entry name" value="MALPROTEIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="31"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="176" mass="19146" checksum="CB554F1BB27E997C" modified="2019-09-18" version="1">MSAGGAPVPPPPNPAMSFPAPRVTLPAGPDILRTYSGAFVCLEIVFGGLVWILVASSNVPLPLLQGWVMFVSVTAFVCSLLFLGVFLSGVVTQINANWNFLDFAYHFTVFVFYFGAFLLEAATTSLHDLRCNRTMTVQPLLSDNQYNINVAATIFAFVTTACYGCSLGLALRRWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G825</accession>
    <name>A0A4W2G825_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PHD finger protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PHF14</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Histone-binding protein. Binds preferentially to unmodified histone H3 but can also bind to a lesser extent to histone H3 trimethylated at 'Lys-9' (H3K9me3) as well as to histone H3 monomethylated at 'Lys-27' (H3K27ac) and trimethylated at 'Lys-27' (H3K27me3). Represses PDGFRA expression, thus playing a role in regulation of mesenchymal cell proliferation. Suppresses the expression of CDKN1A/p21 by reducing the level of trimethylation of histone H3 'Lys-4', leading to enhanced proliferation of germinal center B cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2G825"/>
    <dbReference type="STRING" id="30522.A0A4W2G825"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038258.1">
        <property type="protein sequence ID" value="ENSBIXP00000041424.1"/>
        <property type="gene ID" value="ENSBIXG00000002087.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046470.1">
        <property type="protein sequence ID" value="ENSBIXP00005013038.1"/>
        <property type="gene ID" value="ENSBIXG00005006867.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156923"/>
    <dbReference type="OMA" id="KCGVAVH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002314">
        <property type="term" value="P:germinal center B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048286">
        <property type="term" value="P:lung alveolus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060916">
        <property type="term" value="P:mesenchymal cell proliferation involved in lung development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000791">
        <property type="term" value="P:negative regulation of mesenchymal cell proliferation involved in lung development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000584">
        <property type="term" value="P:negative regulation of platelet-derived growth factor receptor-alpha signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15674">
        <property type="entry name" value="ePHD_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15561">
        <property type="entry name" value="PHD1_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15562">
        <property type="entry name" value="PHD2_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15563">
        <property type="entry name" value="PHD3_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1150:FF:000002">
        <property type="entry name" value="PHD finger protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000059">
        <property type="entry name" value="PHD finger protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000086">
        <property type="entry name" value="PHD finger protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF150">
        <property type="entry name" value="PHD FINGER PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="315"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="19">
        <location>
            <begin position="378"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="721"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="864"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="22"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="827"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="626"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="832"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="992" mass="112049" checksum="222DB4047F104847" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GFJ0</accession>
    <name>A0A4W2GFJ0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">ATP-binding cassette sub-family C member 5</fullName>
            <ecNumber evidence="6">7.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">Multidrug resistance-associated protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">ABCC5</name>
    </gene>
    <organism evidence="34 36">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35 36" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2S)-2-[5-amino-1-(beta-D-ribosyl)imidazole-4-carboxamido]succinate(in) + ATP + H2O = (2S)-2-[5-amino-1-(beta-D-ribosyl)imidazole-4-carboxamido]succinate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66752"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167466"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:66753"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3',5'-cyclic AMP(in) + ATP + H2O = 3',5'-cyclic AMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:66185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>3',5'-cyclic GMP(in) + ATP + H2O = 3',5'-cyclic GMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:66189"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-acetyl-L-aspartate(in) + ATP + H2O = N-acetyl-L-aspartate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66744"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16953"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:66745"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N-acetyl-L-aspartyl-L-glutamate(in) + ATP + H2O = N-acetyl-L-aspartyl-L-glutamate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66728"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:76931"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:66729"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(in) + ATP + H2O = N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66732"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:76935"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:66733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027395467.1">
        <property type="nucleotide sequence ID" value="XM_027539666.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395474.1">
        <property type="nucleotide sequence ID" value="XM_027539673.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395482.1">
        <property type="nucleotide sequence ID" value="XM_027539681.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A4W2GFJ0"/>
    <dbReference type="STRING" id="30522.A0A4W2GFJ0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034923.1">
        <property type="protein sequence ID" value="ENSBIXP00000020709.1"/>
        <property type="gene ID" value="ENSBIXG00000023973.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028241.1">
        <property type="protein sequence ID" value="ENSBIXP00005016761.1"/>
        <property type="gene ID" value="ENSBIXG00005003430.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891532"/>
    <dbReference type="CTD" id="10057"/>
    <dbReference type="GeneTree" id="ENSGT00940000155470"/>
    <dbReference type="OMA" id="QVTDAWT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901505">
        <property type="term" value="F:carbohydrate derivative transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034634">
        <property type="term" value="F:glutathione transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022884">
        <property type="term" value="F:macromolecule transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015216">
        <property type="term" value="F:purine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070730">
        <property type="term" value="P:cAMP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070731">
        <property type="term" value="P:cGMP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098838">
        <property type="term" value="P:folate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035351">
        <property type="term" value="P:heme transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030213">
        <property type="term" value="P:hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18592">
        <property type="entry name" value="ABC_6TM_MRP5_8_9_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18599">
        <property type="entry name" value="ABC_6TM_MRP5_8_9_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000012">
        <property type="entry name" value="ATP binding cassette subfamily C member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000015">
        <property type="entry name" value="multidrug resistance-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000605">
        <property type="entry name" value="multidrug resistance-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF196">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="18 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-1278">Translocase</keyword>
    <keyword evidence="10 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 31" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="857"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="913"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="993"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="1104"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="33">
        <location>
            <begin position="180"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="32">
        <location>
            <begin position="562"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="33">
        <location>
            <begin position="860"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="32">
        <location>
            <begin position="1193"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="501"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="30">
        <location>
            <begin position="514"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="795"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00069159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00082793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1437" mass="161138" checksum="B02A86433864F6CC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GFV3</accession>
    <name>A0A4W2GFV3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SH3 domain binding kinase family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SBK3</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GFV3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022350.1">
        <property type="protein sequence ID" value="ENSBIXP00000034511.1"/>
        <property type="gene ID" value="ENSBIXG00000017507.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028429.1">
        <property type="protein sequence ID" value="ENSBIXP00005016879.1"/>
        <property type="gene ID" value="ENSBIXG00005020383.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154852"/>
    <dbReference type="OMA" id="RDQYHLI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000577">
        <property type="entry name" value="SH3 domain binding kinase family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF39">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="43"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="357" mass="38271" checksum="80D2A372B4A46271" modified="2019-09-18" version="1">MELRDPKKQEDGDPEEDTATALQRLVDLTASRVTPVRSLRAHYRLIRRLGSGSYGHVLLARPRQGGRAVALKLLPRASVFRTTFLREFCVGRCVSSHPGLLQTLAGPLETPRHFAFAQEYAPCGDLSGMLQERGLPELLLKRVVAQLAGALDFLHGRGLVHADVKPDNVLVFDPVCSRVALGDLGLTRPEGSPTPAPPGPLPSAPPELCVLLPPDTLPLRPALDSWGLGVLLFCAATACFPWDVALAPDPEFEVFAGWMTARPQPPRPPPPWDQFAPPALALFQGLLDLDPETRSPPLVVLDFLGDDWGLNGNKDRAGGLGSMSSEGGEEGGASLEEWSEEEDGDSGGRTGTDAGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GI53</accession>
    <name>A0A4W2GI53_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">OR4E2</name>
        <name evidence="16" type="synonym">LOC113899399</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GI53"/>
    <dbReference type="STRING" id="30522.A0A4W2GI53"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048784.1">
        <property type="protein sequence ID" value="ENSBIXP00000019085.1"/>
        <property type="gene ID" value="ENSBIXG00000022777.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030890.1">
        <property type="protein sequence ID" value="ENSBIXP00005018490.1"/>
        <property type="gene ID" value="ENSBIXG00005021868.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163275"/>
    <dbReference type="OMA" id="CACAEIF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15940">
        <property type="entry name" value="7tmA_OR4E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="313" mass="35678" checksum="A15FD5C7B2DA90FA" modified="2019-09-18" version="1">MDALNQTRVTQFVFLGLTDNWVLEILFFMAFSVMYVLTLLGNILIMVTIVFTPRLHIPMYFFLSNLSFIDICHSSVTVPKMLEGLLLERKTISFDNCIAQLFFLHLFACAEIFLLTVMAYDRYVAICVPLHYPNVMDMRVCVQLVFALWLGGTIHSLVQTFLTFRLPYCGPNVIDSYFCDVPPVIKLACTDTYLTGMLIVSNSGTISLFCFLALVTSYMVILVSLRKQSAEGRRKALSTCSAHFMVVALFFGPCIFLYTHPDTNFSSDKVVSVFYTMVTPVLNPLIYTLRNEEVKNAMKRLRQRQVFSMKSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GTD0</accession>
    <name>A0A4W2GTD0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113886428</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388416.1">
        <property type="nucleotide sequence ID" value="XM_027532615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GTD0"/>
    <dbReference type="STRING" id="30522.A0A4W2GTD0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011785.1">
        <property type="protein sequence ID" value="ENSBIXP00000002497.1"/>
        <property type="gene ID" value="ENSBIXG00000008971.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035992.1">
        <property type="protein sequence ID" value="ENSBIXP00005021912.1"/>
        <property type="gene ID" value="ENSBIXG00005005768.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886428"/>
    <dbReference type="GeneTree" id="ENSGT01010000222320"/>
    <dbReference type="OMA" id="YCFFIIS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15405">
        <property type="entry name" value="7tmA_OR8B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="310" mass="34429" checksum="8416826CC4BAB123" modified="2019-09-18" version="1">MAPGNGSFVTEFILVGLTDQPDLQLPLFFLFLVMYMVTVLGNFGLLTLIVLNSHLHTAMYFFLFNLSLIDLCYSSVFTPKMLVDFLSKKNMISYIGCMTQLYFFCFFVISECYVLTSMAYDRYVAICNPLLYNVVMSPKVCSSLMLGSYLMAFSGAMAHTGCMLRLTFCDANTINHYLCDILPVLQLSCTSTYMNELVVFIVVGINIIVPSLTIFVSYGLILSNILHISSTEGRSKAFSTCSSHIIAVSLFFGSGAIMYLKPSSAGSMHVGKISSVFYTNVVPMMNPLTYSLRNKDVKLALKKTLGRGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H1H3</accession>
    <name>A0A4W2H1H3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha-2,8-sialyltransferase 8E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Sialyltransferase 8E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sialyltransferase St8Sia V</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ST8SIA5</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, GP1c and GT3 from GD1a, GT1b, GM1b and GD3 respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ganglioside GQ1c (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GP1c (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87791"/>
            <dbReference type="ChEBI" id="CHEBI:87792"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47593"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381664.1">
        <property type="nucleotide sequence ID" value="XM_027525863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H1H3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009757.1">
        <property type="protein sequence ID" value="ENSBIXP00000028616.1"/>
        <property type="gene ID" value="ENSBIXG00000009912.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053112.1">
        <property type="protein sequence ID" value="ENSBIXP00005025556.1"/>
        <property type="gene ID" value="ENSBIXG00005028065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882761"/>
    <dbReference type="CTD" id="29906"/>
    <dbReference type="GeneTree" id="ENSGT01030000234535"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23990">
        <property type="entry name" value="GT29_ST8SIA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000004">
        <property type="entry name" value="alpha-2,8-sialyltransferase 8E isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF4">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="164"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="376" mass="43924" checksum="C1895D7DB9F6DDAA" modified="2019-09-18" version="1">MRYADPSANRDLLGNRTLLFIFICAFALVTLLQQILYGRNYIKRYFEFYEGPFEYNSTRCLELRHEILEVKVLSMVKQSELFDRWKSLQMCKWAMNISEANQFKSTLSRCCNAPSFLFTTQKNTPLGTKLKYEVDTSGIYHINQEIFRMFPKDMPYYRSQFKKCAVVGNGGILKNSRCGREINSADFVFRCNLPPISEKYTMDVGVKTDVVTVNPSIITERFHKLEKWRRPFYRVLQLYENASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYYFHPQYLVNVSRYWLSLGVRAKRISTGLILATAALELCEEVHLFGFWAFPMNPSGLYITHHYYDNVKPRPGFHAMPSEIFNFLHLHSRGILRVHTGTCSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H8Y0</accession>
    <name>A0A4W2H8Y0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TATA box-binding protein-like 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TATA box-binding protein-related factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">TBP-like factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">TBP-related protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TBPL1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds TFIIA and TFIIB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027407414.1">
        <property type="nucleotide sequence ID" value="XM_027551613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H8Y0"/>
    <dbReference type="SMR" id="A0A4W2H8Y0"/>
    <dbReference type="STRING" id="30522.A0A4W2H8Y0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027674.1">
        <property type="protein sequence ID" value="ENSBIXP00000015979.1"/>
        <property type="gene ID" value="ENSBIXG00000020295.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042274.1">
        <property type="protein sequence ID" value="ENSBIXP00005026203.1"/>
        <property type="gene ID" value="ENSBIXG00005004933.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898449"/>
    <dbReference type="CTD" id="9519"/>
    <dbReference type="GeneTree" id="ENSGT00940000155712"/>
    <dbReference type="OMA" id="NCEYEPE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001673">
        <property type="term" value="C:male germ cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007289">
        <property type="term" value="P:spermatid nucleus differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04517">
        <property type="entry name" value="TLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000005">
        <property type="entry name" value="TATA box-binding protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000009">
        <property type="entry name" value="TatA box-binding protein-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015445">
        <property type="entry name" value="TBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="186" mass="20887" checksum="2CDD9F5E7BDDE63F" modified="2019-09-18" version="1">MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H964</accession>
    <name>A0A4W2H964_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHD finger protein 5A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PHF5A</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHF5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396474.1">
        <property type="nucleotide sequence ID" value="XM_027540673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H964"/>
    <dbReference type="SMR" id="A0A4W2H964"/>
    <dbReference type="STRING" id="30522.A0A4W2H964"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004687.1">
        <property type="protein sequence ID" value="ENSBIXP00000030231.1"/>
        <property type="gene ID" value="ENSBIXG00000012108.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000011.1">
        <property type="protein sequence ID" value="ENSBIXP00005027676.1"/>
        <property type="gene ID" value="ENSBIXG00005030276.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892174"/>
    <dbReference type="CTD" id="84844"/>
    <dbReference type="GeneTree" id="ENSGT00390000018518"/>
    <dbReference type="OMA" id="AYYCWEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005345">
        <property type="entry name" value="PHF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13120">
        <property type="entry name" value="PHD FINGER-LIKE DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03660">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016468">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="110" mass="12405" checksum="90F7469DE7292BF7" modified="2019-09-18" version="1">MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HD98</accession>
    <name>A0A4W2HD98_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromodomain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BRD3</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Homodimer. Interacts with E2F1. Interacts with (acetylated) STAT3; promoting STAT3 recruitment to chromatin. Interacts with CTCF; promoting BRD2 recruitment to chromatin.</text>
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        <text evidence="5">Belongs to the BET family.</text>
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        <property type="gene ID" value="ENSBIXG00005030119.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153385"/>
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    </dbReference>
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    </dbReference>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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        <source>
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        <source>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <sequence length="1040" mass="115889" checksum="B67833F04785E202" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HIK5</accession>
    <name>A0A4W2HIK5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-2-oxoglutarate aldolase, mitochondrial</fullName>
            <ecNumber evidence="5">4.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Dihydrodipicolinate synthase-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Probable 2-keto-4-hydroxyglutarate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HOGA1</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the final step in the metabolic pathway of hydroxyproline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(4R)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:30687"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:62213"/>
            <dbReference type="EC" id="4.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(4S)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:35639"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:71685"/>
            <dbReference type="EC" id="4.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the DapA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027384252.1">
        <property type="nucleotide sequence ID" value="XM_027528451.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384253.1">
        <property type="nucleotide sequence ID" value="XM_027528452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HIK5"/>
    <dbReference type="SMR" id="A0A4W2HIK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003300.1">
        <property type="protein sequence ID" value="ENSBIXP00000006668.1"/>
        <property type="gene ID" value="ENSBIXG00000012766.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010224.1">
        <property type="protein sequence ID" value="ENSBIXP00005029650.1"/>
        <property type="gene ID" value="ENSBIXG00005009628.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884185"/>
    <dbReference type="CTD" id="112817"/>
    <dbReference type="GeneTree" id="ENSGT00530000063604"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008700">
        <property type="term" value="F:(R,S)-4-hydroxy-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000153">
        <property type="entry name" value="4-hydroxy-2-oxoglutarate aldolase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="16">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="16">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="327" mass="35217" checksum="153314DCD1F0D587" modified="2019-09-18" version="1">MLVPRVWSSVRLGLSRVLSRTLRGWPSGEGRGMDLSGIYPPVTTPFTATAEVDYGKLEENLHKLGTLPFRGFVVQGSNGEFPFLTSSERLEVVSRARQALPKDKLLLAGSGCESTQATVEMTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSPVPVVLYSVPANTGLDLPVDAVVTLSQHPNIVGIKDSGGDVTRIGLIVHKTRSQDFQVLAGSAGFLLASYAIGAVGGVCALANVLGSQVCQLERLCLTGQWEDAQKLQHRLIEPNTAVTRRFGIPGLKKTMDWFGYYGGPCRSPLQELSPAQEEALRLDFASNGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HJ99</accession>
    <name>A0A4W2HJ99_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="9">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OXCT1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:24565"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetoacetate + succinyl-CoA = acetoacetyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:25480"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:25481"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027375829.1">
        <property type="nucleotide sequence ID" value="XM_027520028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HJ99"/>
    <dbReference type="SMR" id="A0A4W2HJ99"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018823.1">
        <property type="protein sequence ID" value="ENSBIXP00000010147.1"/>
        <property type="gene ID" value="ENSBIXG00000002373.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009430.1">
        <property type="protein sequence ID" value="ENSBIXP00005029929.1"/>
        <property type="gene ID" value="ENSBIXG00005009957.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878884"/>
    <dbReference type="CTD" id="5019"/>
    <dbReference type="GeneTree" id="ENSGT00390000009130"/>
    <dbReference type="UniPathway" id="UPA00929">
        <property type="reaction ID" value="UER00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000001">
        <property type="entry name" value="Succinyl-coa:3-ketoacid-coenzyme a transferase subunit b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000002">
        <property type="entry name" value="Succinyl-CoA:3-ketoacid-coenzyme A transferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004163">
        <property type="entry name" value="CoA_transf_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF30">
        <property type="entry name" value="SUCCINYL-COA:3-KETOACID COENZYME A TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01273">
        <property type="entry name" value="COA_TRANSF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="10">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="520" mass="56453" checksum="0535FC9E18E24009" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HRB8</accession>
    <name>A0A4W2HRB8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Juxtaposed with another zinc finger protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">JAZF1</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a transcriptional corepressor of orphan nuclear receptor NR2C2. Inhibits expression of the gluconeogenesis enzyme PCK2 through inhibition of NR2C2 activity. Also involved in transcriptional activation of NAMPT by promoting expression of PPARA and PPARD. Plays a role in lipid metabolism by suppressing lipogenesis, increasing lipolysis and decreasing lipid accumulation in adipose tissue. Plays a role in glucose homeostasis by improving glucose metabolism and insulin sensitivity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with NR2C2 (via ligand-binding region).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027395211.1">
        <property type="nucleotide sequence ID" value="XM_027539410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HRB8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025278.1">
        <property type="protein sequence ID" value="ENSBIXP00000014414.1"/>
        <property type="gene ID" value="ENSBIXG00000019120.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018295.1">
        <property type="protein sequence ID" value="ENSBIXP00005033949.1"/>
        <property type="gene ID" value="ENSBIXG00005002099.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891411"/>
    <dbReference type="CTD" id="221895"/>
    <dbReference type="GeneTree" id="ENSGT00390000003635"/>
    <dbReference type="OMA" id="CLWHGCG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000619">
        <property type="entry name" value="juxtaposed with another zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003264">
        <property type="entry name" value="Juxtaposed with another zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="89"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="243" mass="27079" checksum="878A6EE3D82C0588" modified="2019-09-18" version="1">MTGIAAASFFSNTCRFGGCGLHFPTLADLIEHIEDNHIDTDPRVLEKQELQQPTYVALSYINRFMTDAARREQESLKKKIQPKLSLTLSSSVSRGNVSTPPRHSSGSLTPPVTPPITPSSSFRSSTPTGSEYDEEEVDYEESDSDESWTTESAISSEAILSSMCMNGGEEKPFACPVPGCKKRYKNVNGIKYHAKNGHRTQIRVRKPFKCRCGKSYKTAQGLRHHTINFHPPVSAEIIRKMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HXF0</accession>
    <name>A0A4W2HXF0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RAC3</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027373686.1">
        <property type="nucleotide sequence ID" value="XM_027517885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HXF0"/>
    <dbReference type="SMR" id="A0A4W2HXF0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016841.1">
        <property type="protein sequence ID" value="ENSBIXP00000032174.1"/>
        <property type="gene ID" value="ENSBIXG00000014648.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020017.1">
        <property type="protein sequence ID" value="ENSBIXP00005034699.1"/>
        <property type="gene ID" value="ENSBIXG00005015712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877620"/>
    <dbReference type="CTD" id="5881"/>
    <dbReference type="GeneTree" id="ENSGT00940000153500"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021894">
        <property type="term" value="P:cerebral cortex GABAergic interneuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048873">
        <property type="term" value="P:homeostasis of number of cells within a tissue"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033630">
        <property type="term" value="P:positive regulation of cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
        <property type="term" value="P:positive regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098974">
        <property type="term" value="P:postsynaptic actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022604">
        <property type="term" value="P:regulation of cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014041">
        <property type="term" value="P:regulation of neuron maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150052">
        <property type="term" value="P:regulation of postsynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051932">
        <property type="term" value="P:synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01871">
        <property type="entry name" value="Rac1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000088">
        <property type="entry name" value="Ras-related C3 botulinum toxin substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="192" mass="21379" checksum="560BBC26BB7CDF4A" modified="2019-09-18" version="1">MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPHTPILLVGTKLDLRDDKDTIERLRDKKLAPITYPQGLAMAREIGSVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKPGKKCTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I5K9</accession>
    <name>A0A4W2I5K9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OSBPL2</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I5K9"/>
    <dbReference type="STRING" id="30522.A0A4W2I5K9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027979.1">
        <property type="protein sequence ID" value="ENSBIXP00000036923.1"/>
        <property type="gene ID" value="ENSBIXG00000020441.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030013.1">
        <property type="protein sequence ID" value="ENSBIXP00005039075.1"/>
        <property type="gene ID" value="ENSBIXG00005021374.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158762"/>
    <dbReference type="OMA" id="MSEPLQY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098831">
        <property type="term" value="C:presynaptic active zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099509">
        <property type="term" value="P:regulation of presynaptic cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010807">
        <property type="term" value="P:regulation of synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000005">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF153">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="482" mass="54328" checksum="BF68039C012FDCA5" modified="2019-09-18" version="1">MNGEEEFFDAVTGFDSDNSSGEFSEANQRLTGVIHVDTSKSNGIGKVGDGPPQENGIQKHRTSLPAPMFTRSDFSVWSILKKCIGLELSKITMPIAFNEPLSFLQRITEYMEHVYLIHRASRQPQSLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRFISEQVSHHPPISAFYSEGLHQDFLFHGSIYPKLKFWGKSVEAEPRGTITLELLKHKEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGDKCVLHFKPCGLFGKELHKVEGYIQDKSKKKLFVIYGKWTECLWGIDPVTYESFRKQERRGDSLRKTKPDDGPEKADCDVADSVPESQETVQVIPGSKLLWRVNTRPPNSAQMYNFTSFTVSLNELETGMEKILAPTDCRLRPDIRGMENGNMGACSHWGLTGRPALATRVVQGRNVSLLTEHTSYLLDVLLKPSVGKSCKASSLLQATSFINKIRLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IGA3</accession>
    <name>A0A4W2IGA3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF3B</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-based molecular motor that transport intracellular cargos, such as vesicles, organelles and protein complexes. Uses ATP hydrolysis to generate force to bind and move along the microtubule. Plays a role in cilia formation. Involved in photoreceptor integrity and opsin trafficking in rod photoreceptors. Transports vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit GRIN2A into neuronal dendrites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2IGA3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029859.1">
        <property type="protein sequence ID" value="ENSBIXP00000017035.1"/>
        <property type="gene ID" value="ENSBIXG00000003996.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039138.1">
        <property type="protein sequence ID" value="ENSBIXP00005043092.1"/>
        <property type="gene ID" value="ENSBIXG00005026710.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153739"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008088">
        <property type="term" value="P:axo-dendritic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000017">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF76">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="9"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="374"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="657"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="425"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="668"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="705" mass="80192" checksum="C299DF17C8DC4167" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IL17</accession>
    <name>A0A4W2IL17_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-type lectin domain family 2 member L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CLEC2L</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027394659.1">
        <property type="nucleotide sequence ID" value="XM_027538858.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027394660.1">
        <property type="nucleotide sequence ID" value="XM_027538859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IL17"/>
    <dbReference type="STRING" id="30522.A0A4W2IL17"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048142.1">
        <property type="protein sequence ID" value="ENSBIXP00000037993.1"/>
        <property type="gene ID" value="ENSBIXG00000021712.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055563.1">
        <property type="protein sequence ID" value="ENSBIXP00005045956.1"/>
        <property type="gene ID" value="ENSBIXG00005030966.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891145"/>
    <dbReference type="CTD" id="154790"/>
    <dbReference type="GeneTree" id="ENSGT00940000162503"/>
    <dbReference type="OMA" id="CHTHEAS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47498">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47498:SF1">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="107"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="214" mass="23908" checksum="458F4D40BB720714" modified="2019-09-18" version="1">MEPAREPPARTRPPPPPAVRPAPAPAAPRPRSPAEAEGRSPEGLLRRSGSGYEGSTSWKAALEDTTTRLLLGAIAVLLFAILVVMSILASKGCIKCEAPCPEDWLLYGRKCYFFSEEPRDWNTGRQYCHSHEAALAVIQSQKELEFMFKFTRREPWIGLRRVGDEFHWVNGDPFDPDTFPIAGPGECVFVEPTRLVSTDCLMTRPWVCSKMAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IMF9</accession>
    <name>A0A4W2IMF9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SLIT-ROBO Rho GTPase-activating protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rho GTPase-activating protein 14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">WAVE-associated Rac GTPase-activating protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SRGAP3</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Homodimer. Forms a heterooligomer with SRGAP1 and SRGAP2 through its F-BAR domain. Interacts with WASF1. Probably interacts with ROBO1. Interacts with FASLG.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379489.1">
        <property type="nucleotide sequence ID" value="XM_027523688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IMF9"/>
    <dbReference type="SMR" id="A0A4W2IMF9"/>
    <dbReference type="STRING" id="30522.A0A4W2IMF9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013530.1">
        <property type="protein sequence ID" value="ENSBIXP00000027372.1"/>
        <property type="gene ID" value="ENSBIXG00000008192.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042530.1">
        <property type="protein sequence ID" value="ENSBIXP00005044835.1"/>
        <property type="gene ID" value="ENSBIXG00005005347.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880975"/>
    <dbReference type="CTD" id="9901"/>
    <dbReference type="GeneTree" id="ENSGT00950000182824"/>
    <dbReference type="OMA" id="MEAFIKX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07684">
        <property type="entry name" value="F-BAR_srGAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04383">
        <property type="entry name" value="RhoGAP_srGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11955">
        <property type="entry name" value="SH3_srGAP1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000013">
        <property type="entry name" value="SLIT-ROBO Rho GTPase activating protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000020">
        <property type="entry name" value="SLIT-ROBO Rho GTPase activating protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000005">
        <property type="entry name" value="SLIT-ROBO Rho GTPase-activating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
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        <property type="entry name" value="SLIT-ROBO RHO GTPASE ACTIVATING PROTEIN"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="12">
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="1047"/>
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        </location>
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        <location>
            <begin position="359"/>
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            <begin position="809"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="926"/>
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        <location>
            <begin position="937"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1060"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1099" mass="124502" checksum="2AA0FD01BC8760EE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BJR7</accession>
    <name>A0A4W2BJR7_BOBOX</name>
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        <submittedName>
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        <lineage>
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            <taxon>Eutheria</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="component" value="Chromosome 25"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="147" mass="15891" checksum="D9676F5381D4C8D4" modified="2019-09-18" version="1">MSSEPPPPSQPPTHQSSTGLLDTQQARDRSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPMEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQF0</accession>
    <name>A0A4W2BQF0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4</fullName>
            <ecNumber evidence="16">2.4.3.2</ecNumber>
            <ecNumber evidence="17">2.4.3.4</ecNumber>
            <ecNumber evidence="23">2.4.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Alpha 2,3-sialyltransferase IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Gal-beta-1,4-GlcNAc-alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">N-acetyllactosaminide alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">ST3Gal IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Sialyltransferase 4C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">LOC113885942</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36 37" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:139596"/>
            <dbReference type="EC" id="2.4.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:136588"/>
            <dbReference type="ChEBI" id="CHEBI:136589"/>
            <dbReference type="EC" id="2.4.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:52381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52316"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="EC" id="2.4.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:52317"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a ganglioside GA1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27938"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:47561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a ganglioside GM1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1a (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77709"/>
            <dbReference type="ChEBI" id="CHEBI:78445"/>
            <dbReference type="EC" id="2.4.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18022"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a ganglioside GT1c (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GQ1c (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87789"/>
            <dbReference type="ChEBI" id="CHEBI:87791"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47589"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a neolactoside nLc4Cer + CMP-N-acetyl-beta-neuraminate = a neolactoside IV(3)-alpha-NeuAc-nLc4Cer + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:90376"/>
            <dbReference type="ChEBI" id="CHEBI:90390"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:65433"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a neolactoside nLc4Cer(d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a neolactoside IV(3)-alpha-NeuAc-nLc4Cer(d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:58665"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.4.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:18914"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus</location>
            <location evidence="15">Golgi stack membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.2" evidence="16"/>
    <dbReference type="EC" id="2.4.3.4" evidence="17"/>
    <dbReference type="EC" id="2.4.3.6" evidence="23"/>
    <dbReference type="RefSeq" id="XP_027387605.1">
        <property type="nucleotide sequence ID" value="XM_027531804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQF0"/>
    <dbReference type="SMR" id="A0A4W2BQF0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014237.1">
        <property type="protein sequence ID" value="ENSBIXP00000001346.1"/>
        <property type="gene ID" value="ENSBIXG00000028654.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052857.1">
        <property type="protein sequence ID" value="ENSBIXP00005025117.1"/>
        <property type="gene ID" value="ENSBIXG00005004780.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885942"/>
    <dbReference type="GeneTree" id="ENSGT00940000158893"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047288">
        <property type="term" value="F:beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003836">
        <property type="term" value="F:beta-galactoside (CMP) alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008118">
        <property type="term" value="F:N-acetyllactosaminide alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23982">
        <property type="entry name" value="GT29_ST3GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000005">
        <property type="entry name" value="ST3 beta-galactoside alpha-2,3-sialyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051142">
        <property type="entry name" value="Glycosyltransferase_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13713:SF57">
        <property type="entry name" value="CMP-N-ACETYLNEURAMINATE-BETA-GALACTOSAMIDE-ALPHA-2,3-SIALYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13713">
        <property type="entry name" value="SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="34" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="34">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610264" description="CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4" evidence="34">
        <location>
            <begin position="27"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="33">
        <location>
            <begin position="120"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00072813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00075868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00076295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00076532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00081234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00082801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="333" mass="38423" checksum="4C4BAD3C9F5DD16E" modified="2019-09-18" version="1">MISKSRWKLLAMLALFLAVMVWYSISREDRYIELFYFPVPGKKEPCLQGEAEKMVSKLFGNYSREQPFFLQLKDYFWVKTPSLYELPYGTKGSEDLLLRVLAITSYSIPESIQSLKCRRCVVVGNGHRLRNSSLGEAINKYDVVIRLNSAPVAGYEQDVGSKTTMRLFYPESAHFNPKVEDNPDTLLVLVAFKAMDFHWIESILSDKKRVRKGFWKQPPLIWDVNPKQIRILNPFYMEIAADKLLSLPIHQPHKIKQKPTTGLLAITLALHLCDLVHIAGFGYPDAHQKKQSIHYYEYITLKSMMWSGHNVSQEALAIKRMLEIGAVKNLTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQI0</accession>
    <name>A0A4W2BQI0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MAS related GPR family member G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MRGPRG</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family. Mas subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQI0"/>
    <dbReference type="STRING" id="30522.A0A4W2BQI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014169.1">
        <property type="protein sequence ID" value="ENSBIXP00000001378.1"/>
        <property type="gene ID" value="ENSBIXG00000028637.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034972.1">
        <property type="protein sequence ID" value="ENSBIXP00005021236.1"/>
        <property type="gene ID" value="ENSBIXG00005024242.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234639"/>
    <dbReference type="OMA" id="NGLVLWH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000193">
        <property type="entry name" value="Mas-related G-protein coupled receptor member E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334:SF32">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR MEMBER G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02108">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="298" mass="31726" checksum="2768DE36E6BD51EB" modified="2019-09-18" version="1">MATSQPASMLGIWGTFSSVVFYLTLAVGLGGLLGNALVLWHLGFHIKKGPFAVYVLHLAAADFLFLGCQLTFSAVQAALGSGHSLYFAVTFVAFSVGLWLLAAFSAECCLSDLFPACYQGCRPRHTLGVVCGLSWALAPPAVLLTANACGLLRESMRLLACLRYHAASVAGLLALACAAFAAGLVLLVWGACCSQRQRPRFYGAVLGSELLLLVCGLPYLLCWTLRPYLPSFLLSTFFPLATLLACVHCSGRPLIYFMVGRQPGRREPLRVVLRRSLGEGAQLGAGGVSLPVGRVWGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQS2</accession>
    <name>A0A4W2BQS2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQS2"/>
    <dbReference type="STRING" id="30522.A0A4W2BQS2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012068.1">
        <property type="protein sequence ID" value="ENSBIXP00000002363.1"/>
        <property type="gene ID" value="ENSBIXG00000008842.1"/>
    </dbReference>
    <dbReference type="OMA" id="SICANPG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048020">
        <property type="term" value="F:CCR chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070098">
        <property type="term" value="P:chemokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048245">
        <property type="term" value="P:eosinophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000002">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF149">
        <property type="entry name" value="REGAKINE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2 4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021452922" description="C-C motif chemokine" evidence="4">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="5">
        <location>
            <begin position="24"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="87" mass="10044" checksum="FE1418A1C0AD6660" modified="2019-09-18" version="1">FKWFSLALYFHLTVLYLAEPAGNMRVCCFSSVTRKIPLSLVKNYERTGDKCPQEAVIFQTRSGRSICANPGQAWVQKYIEYLDQMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSI3</accession>
    <name>A0A4W2BSI3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSI3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010657.1">
        <property type="protein sequence ID" value="ENSBIXP00000003054.1"/>
        <property type="gene ID" value="ENSBIXG00000009467.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000346">
        <property type="entry name" value="RAB31, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="195" mass="21525" checksum="ED8008264ABD6EC9" modified="2019-09-18" version="1">MMAIRELKVCLLGDTGVGKSSIVCRFVQDHFDHNISPTIGASFMTKTVPCGNELHKFLIWDTAGQERFHSLAPMYYRGSAAAVIVYDITKQDSFHTLKKWVKELKEHGPENIVMAIAGNKCDLSDIREVPLKDAKEYAESIGAVVVETSAKNAINIEELFQGISRQIPPLDPHENGNSGSIKLGKPTSQDFSTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVW5</accession>
    <name>A0A4W2BVW5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PSMG2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381414.1">
        <property type="nucleotide sequence ID" value="XM_027525613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVW5"/>
    <dbReference type="SMR" id="A0A4W2BVW5"/>
    <dbReference type="STRING" id="30522.A0A4W2BVW5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010429.1">
        <property type="protein sequence ID" value="ENSBIXP00000003154.1"/>
        <property type="gene ID" value="ENSBIXG00000000352.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009334.1">
        <property type="protein sequence ID" value="ENSBIXP00005029982.1"/>
        <property type="gene ID" value="ENSBIXG00005006712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882650"/>
    <dbReference type="CTD" id="56984"/>
    <dbReference type="GeneTree" id="ENSGT00390000018415"/>
    <dbReference type="OMA" id="WKEHTGE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10900:FF:000001">
        <property type="entry name" value="Proteasome assembly chaperone 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10900:FF:000003">
        <property type="entry name" value="Proteasome assembly chaperone 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="264" mass="29224" checksum="1C7D67BBB8BE5D11" modified="2019-09-18" version="1">MFVPSGDSVPDLAGCTLLMPAVSVGNVGQLAIDLIISTLNMHKIGYFYTDCLVPMVGNNPYATAEENSAELSINAEVYALPSKKLVALQLRSIFIKYKSKSFCEKLLSWVKCSGCAKVVVLSSSHSYHRNDLQLRSTPFRYLLTPSMQKSVQNKIQSLNWEEMEKTPCIPEIDDSEFCVRVPGGGITKLLYDEGCSKEIPIAILLKFVSEGDNIPDALGLVEYLNEWLQIIKPHCEDPTASSLPWKMPSSWRLLFGSGLPPALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C139</accession>
    <name>A0A4W2C139_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C139"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005752.1">
        <property type="protein sequence ID" value="ENSBIXP00000005442.1"/>
        <property type="gene ID" value="ENSBIXG00000011629.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="45"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="366" mass="42012" checksum="7BDF745CBB6D62D5" modified="2019-09-18" version="1">MECEVKWALESITTNKANGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALISHTSKVMLKILQARLQQYVNREIPDVRAAFRKGRGIRDQIANIRWIMEKAREFQKNNYFYFIDYAKAFDCVDHNKLWKILQEMGIPDRLICLLRNLYAGQEATVRTGYGTTDWFQIGKGVRQGRILSPRLFNFYAEYIRRNAGLEEQAGIKITGRNINNLRYADDITLMAESEEELKSLLMKVKVESEKVGLMLNIQKTKITALGPTTSWEIDGETVETVSDFIFGGSKITADGDCIHEIKRRLLLESKIMSNLDSIFKSRDITLPTKVHLVKAMVFPVVMYGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C257</accession>
    <name>A0A4W2C257_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysosomal thioesterase PPT2</fullName>
            <ecNumber evidence="8">3.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PPT2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the cleavage of thioester bonds from S-palmitoyl-CoA or S-palmitoyl-N-acetylcysteamine (unbranched structures) but does not have activity against palmitoylcysteine or palmitoylated proteins, branched structures or bulky head groups. Conversely, hydrolyzes both long and short chain fatty acyl-CoA substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-hexadecanoyl-N-acetylcysteamine + H2O = N-acetylcysteamine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:84099"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74410"/>
            <dbReference type="ChEBI" id="CHEBI:233601"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the palmitoyl-protein thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.2" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C257"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004973.1">
        <property type="protein sequence ID" value="ENSBIXP00000005834.1"/>
        <property type="gene ID" value="ENSBIXG00000011966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098599">
        <property type="term" value="F:palmitoyl hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000037">
        <property type="entry name" value="Lysosomal thioesterase PPT2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF27">
        <property type="entry name" value="LYSOSOMAL THIOESTERASE PPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021229082" description="Lysosomal thioesterase PPT2" evidence="12">
        <location>
            <begin position="33"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="309" mass="34728" checksum="E96EC67FD96198CE" modified="2019-09-18" version="1">MLGLPERRLPSAEFLLLLPFLLLLLLLLPAAPAPHRAAYKPVIVVHGLFDSSYSFRHLLEYINETHPGTAVTVLDLFDGRESLRPLWEQVQGFREAVAPIMAKALQGVHLICYSQGGLVCRALLSVMDEHNVDSFISLSSPQMGQYGDTNYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATAWRKNFLRLGRLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEKQLVSPLELLSLSAHTVVLSAPDTEAEGSTLWGGGLGRQRELTLSLPMSFFHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3N0</accession>
    <name>A0A4W2C3N0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113881648</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3N0"/>
    <dbReference type="STRING" id="30522.A0A4W2C3N0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001971.1">
        <property type="protein sequence ID" value="ENSBIXP00000007380.1"/>
        <property type="gene ID" value="ENSBIXG00000013338.1"/>
    </dbReference>
    <dbReference type="OMA" id="SAIYAYV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15947">
        <property type="entry name" value="7tmA_OR2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="35176" checksum="B1E2536F09BC542A" modified="2019-09-18" version="1">MEQKNGSSFTGFILLGFSDRPQLELVLFVVLLIFYIFTLLGNTTIIALSYLDPRLHTPMYFFLSNLSFLDMCYTTSIVPQFLFNLSGADKSISFGGCVVQMYISLALGCTECILLGVMAFDRYAAVCRPLHYTVIMHPRLCALMASASWVTGFAISLLQTVLIFLLPLCGRNKLDHFFCEIPPFLKLACVDTTMNVYATFFASIIILLTPVSLIMFSYGRIVRAVLRIKSTTGRRKAFGTCGSHFTVVSLFFGTAIYVYFQPSSSDNQDQEKFMSLFYTVIIPMTNPLIYTLRNRDVKGAMKKVLWKDPDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3X7</accession>
    <name>A0A4W2C3X7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor IIIB 90 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">B-related factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BRF1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1. Interacts with MAF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3X7"/>
    <dbReference type="SMR" id="A0A4W2C3X7"/>
    <dbReference type="STRING" id="30522.A0A4W2C3X7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001848.1">
        <property type="protein sequence ID" value="ENSBIXP00000007440.1"/>
        <property type="gene ID" value="ENSBIXG00000013381.1"/>
    </dbReference>
    <dbReference type="OMA" id="EPPCKVM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000012">
        <property type="entry name" value="Putative transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000001">
        <property type="entry name" value="transcription factor IIIB 90 kDa subunit isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="17">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="544"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="697" mass="76001" checksum="8012D94C6386D95E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6H3</accession>
    <name>A0A4W2C6H3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Centromere protein V</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Proline-rich protein 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CENPV</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Required for distribution of pericentromeric heterochromatin in interphase nuclei and for centromere formation and organization, chromosome alignment and cytokinesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375269.1">
        <property type="nucleotide sequence ID" value="XM_027519468.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00000007172.1"/>
        <property type="gene ID" value="ENSBIXG00000013157.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045325.1">
        <property type="protein sequence ID" value="ENSBIXP00005010741.1"/>
        <property type="gene ID" value="ENSBIXG00005015313.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878410"/>
    <dbReference type="CTD" id="201161"/>
    <dbReference type="GeneTree" id="ENSGT00390000003183"/>
    <dbReference type="OMA" id="FNGNEWE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032467">
        <property type="term" value="P:positive regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033044">
        <property type="term" value="P:regulation of chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.70:FF:000001">
        <property type="entry name" value="Centromere protein V"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF4">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="22">
        <location>
            <begin position="126"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00077491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="253" mass="27703" checksum="5C487FD66499E1AC" modified="2019-09-18" version="1">MRRARSGGATKPRGQKRPGTSRAPASAPSADRARRPAVQAGGGSRAAARQPAAKRRSPPVQEAGPGEPPPVPPLPPPPPSALTASELDLGEQRERWETFQKRQRLSFEGAAKLLLDTYEYQGLVKHTGGCHCGAVRFEVWASADLHIFDCNCSICKKKQNKHFIVPASRFKLLKGADSITTYTFNTHKAQHTFCKRCGVQSFYSPRSNPGGFGIAPHCLDEGTVRSVVVEEFNGSDWEKAMKEHKTIKNMSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7I2</accession>
    <name>A0A4W2C7I2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7I2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012289.1">
        <property type="protein sequence ID" value="ENSBIXP00000002245.1"/>
        <property type="gene ID" value="ENSBIXG00000008730.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="113"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="401" mass="45557" checksum="8D0E966DDB074334" modified="2019-09-18" version="1">MLLQMALSPSLLWLSSSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDQDNHNGVITHLEPDILECEVKWALESITMNKASGSDGIPVELFQILKDDAVKGLHPICQQIWKTQQSSTFPEDWKRSAFIPIPKKGNAKECSNYRTTALISHASKVMLKILQARPQQYVNRELPDVQAGFRKGRGTRDQIANILRIIEKAREFQKNIYFCFIDYAKAFDCVDHKKLQKILKEMEIPDHLTCLLINLYAGQEATVRTGHGTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLGEAQAGIKIAGRNINNLRYADNTTLMAESEEELKSFLMKVKEESEKIGLKLNIQKMKIMASGPITSWEIDGETVEQCQTLFLGAPKSLQMVIAAMKLKDTYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8D4</accession>
    <name>A0A4W2C8D4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">CD180 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Radioprotective 105 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CD180</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">M-shaped tetramer of two CD180-LY86 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376226.1">
        <property type="nucleotide sequence ID" value="XM_027520425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C8D4"/>
    <dbReference type="SMR" id="A0A4W2C8D4"/>
    <dbReference type="STRING" id="30522.A0A4W2C8D4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043941.1">
        <property type="protein sequence ID" value="ENSBIXP00000009077.1"/>
        <property type="gene ID" value="ENSBIXG00000007726.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019081.1">
        <property type="protein sequence ID" value="ENSBIXP00005010611.1"/>
        <property type="gene ID" value="ENSBIXG00005015201.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879153"/>
    <dbReference type="CTD" id="4064"/>
    <dbReference type="GeneTree" id="ENSGT00940000161183"/>
    <dbReference type="OMA" id="FLRWKYQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046696">
        <property type="term" value="C:lipopolysaccharide receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001875">
        <property type="term" value="F:lipopolysaccharide immune receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002322">
        <property type="term" value="P:B cell proliferation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032497">
        <property type="term" value="P:detection of lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002755">
        <property type="term" value="P:MyD88-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031666">
        <property type="term" value="P:positive regulation of lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000302">
        <property type="entry name" value="CD180 antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041281">
        <property type="entry name" value="LRR_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18831">
        <property type="entry name" value="LRR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610396" description="CD180 antigen" evidence="20">
        <location>
            <begin position="21"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="630"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="21">
        <location>
            <begin position="577"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="661" mass="74261" checksum="24A1509E9E8F9D81" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C957</accession>
    <name>A0A4W2C957_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenosine deaminase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for male fertility and normal male germ cell differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ADAD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C957"/>
    <dbReference type="STRING" id="30522.A0A4W2C957"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043473.1">
        <property type="protein sequence ID" value="ENSBIXP00000008112.1"/>
        <property type="gene ID" value="ENSBIXG00000014007.1"/>
    </dbReference>
    <dbReference type="OMA" id="WCLGDED"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000056">
        <property type="entry name" value="Adenosine deaminase domain-containing 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ADENOSINE DEAMINASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="108"/>
            <end position="174"/>
        </location>
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        <location>
            <begin position="254"/>
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        </location>
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        <location>
            <begin position="1"/>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059681"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061642"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="576" mass="60502" checksum="CF8F7224DFA7A5A7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CB93</accession>
    <name>A0A4W2CB93_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">NOVA alternative splicing regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NOVA1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with PTBP2; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
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            <end position="121"/>
        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="483" mass="49267" checksum="2D4352D3DE9BFCFB" modified="2019-09-18" version="1">MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTVEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQVKIIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBA4</accession>
    <name>A0A4W2CBA4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="26">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">IMPA1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol 1-phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27670"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58433"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:27671"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1D-myo-inositol 2-phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:44152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84142"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44153"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1D-myo-inositol 3-phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30739"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:30740"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1D-myo-inositol 4-phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30735"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58469"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:30736"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 5-phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:44156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84141"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:44157"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1D-myo-inositol 6-phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:44160"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64841"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:44161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-glucose 6-phosphate + H2O = D-glucose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16689"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:16690"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:24057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>adenosine 2'-phosphate + H2O = adenosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:77740"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:37344"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>alpha-D-galactose 1-phosphate + H2O = D-galactose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29315"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="EC" id="3.1.3.94"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:29316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>alpha-D-glucose 1-phosphate + H2O = D-glucose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19933"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:19934"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:35604"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>glycerol 2-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58083"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:13106"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>scyllo-inositol 1-phosphate + H2O = scyllo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:82131"/>
            <dbReference type="ChEBI" id="CHEBI:10642"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:232087"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:82132"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25 26">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 26">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 26">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="26"/>
    <dbReference type="RefSeq" id="XP_027417466.1">
        <property type="nucleotide sequence ID" value="XM_027561665.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027417467.1">
        <property type="nucleotide sequence ID" value="XM_027561666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBA4"/>
    <dbReference type="SMR" id="A0A4W2CBA4"/>
    <dbReference type="STRING" id="30522.A0A4W2CBA4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018788.1">
        <property type="protein sequence ID" value="ENSBIXP00000010129.1"/>
        <property type="gene ID" value="ENSBIXG00000015693.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904472"/>
    <dbReference type="CTD" id="3612"/>
    <dbReference type="OMA" id="ERGLHPW"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103026">
        <property type="term" value="F:fructose-1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008877">
        <property type="term" value="F:glucose-1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004346">
        <property type="term" value="F:glucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047954">
        <property type="term" value="F:glycerol-2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000002">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020552">
        <property type="entry name" value="Inositol_monoPase_Li-sen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF26">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00378">
        <property type="entry name" value="LIIMPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0452">Lithium</keyword>
    <keyword evidence="10 25 26" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 25 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="25">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="277" mass="30056" checksum="773DDB0291AC5977" modified="2019-09-18" version="1">MADPWQECMDYAVTLAGQAGEVVREALKNEMNIMVKSSPADLVTATDQKVEKMLITSIKEKYPSHSFIGEESVAAGEKSILTDNPTWIIDPIDGTTNFVHGFPFVAVSIGFVVNKKMEFGIVYSCLEDKMYTGRKGKGAFCNGQKLQVSHQEDITKSLLVTELGSSRTPETVRIILSNIERLLCLPIHGIRGVGTAALNMCLVAAGAADAYYEMGIHCWDVAGAGIIVTEAGGVLLDVTGGPFDLMSRRVIASSNKTLAERIAKEIQIIPLQRDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CC53</accession>
    <name>A0A4W2CC53_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane-associated progesterone receptor component 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome b5 domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Neuferricin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of a progesterone-binding protein complex. Binds progesterone. Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins. Forms a ternary complex with TMEM97 receptor and low density lipid receptor/LDLR, which increases LDLR-mediated LDL lipoprotein internalization.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Heme-binding protein which promotes neuronal but not astrocyte differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer. Forms stable homodimer through hydrophobic heme-heme stacking interactions. Interacts with FECH; the interaction results in decreased FECH activity. Interacts with EGFR, CYP1A1 and CYP3A4; the interactions require PGRMC1 homodimerization. Interacts with TMEM97 and LDLR; the interaction increases LDL internalization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion outer membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
            <orientation evidence="6">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Smooth endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CC53"/>
    <dbReference type="SMR" id="A0A4W2CC53"/>
    <dbReference type="STRING" id="30522.A0A4W2CC53"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017306.1">
        <property type="protein sequence ID" value="ENSBIXP00000009202.1"/>
        <property type="gene ID" value="ENSBIXG00000014908.1"/>
    </dbReference>
    <dbReference type="OMA" id="GHKHYGP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030868">
        <property type="term" value="C:smooth endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF4">
        <property type="entry name" value="NEUFERRICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0492">Microsome</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="16">
        <location>
            <begin position="38"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="263" mass="29200" checksum="EAD8795DCF969837" modified="2019-09-18" version="1">MPGLGGRGLWLGPAVAVAAAMAAWLMGWWSPRADFRLFIPEELARYRGRPGDPGLYLALLGRVYDVSSGRRHYEPGAHYSGFAGRDASRAFVTGDYSEAGLVDDVSDLSFSEMLTLQNWLSFYEKNYKFIGRVIGRFYGEDGLPTPELTQAEAMMTKGLEADRQELIEKQRFPPCNAEWSFIRGSRFWCSQQSGGVSRDWIGVPRKLFKPGVKPHCVCVRTTGPPSDQSPENPTHRNRGDLDHPNLGEYPGCPPLAITCSVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CE64</accession>
    <name>A0A4W2CE64_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mos1 transposase HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CE64"/>
    <dbReference type="SMR" id="A0A4W2CE64"/>
    <dbReference type="STRING" id="30522.A0A4W2CE64"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018942.1">
        <property type="protein sequence ID" value="ENSBIXP00000010234.1"/>
        <property type="gene ID" value="ENSBIXG00000015754.1"/>
    </dbReference>
    <dbReference type="OMA" id="FITTITH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044547">
        <property type="term" value="F:DNA topoisomerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046975">
        <property type="term" value="F:histone H3K36 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041426">
        <property type="entry name" value="Mos1_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052709">
        <property type="entry name" value="Transposase-MT_Hybrid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001888">
        <property type="entry name" value="Transposase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46060:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46060">
        <property type="entry name" value="MARINER MOS1 TRANSPOSASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17906">
        <property type="entry name" value="HTH_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01359">
        <property type="entry name" value="Transposase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mos1 transposase HTH" evidence="1">
        <location>
            <begin position="5"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="341" mass="40116" checksum="D6D6016C247525D5" modified="2019-09-18" version="1">MEEKKQHFPHIMLYYFKKGKNATETQKKICTVYGEGAVTDRTCQKWFAKFRAGDFSLDHAPRLGRPVEVDSSQIKTLIENNQRYTTREIADILKVSKSSVENHLHQLGYVNRFGVWVPRTLSEKNLLDRISTCDSLLKRNENVPFLKQIVTGDEKWILYNNVEQKGRWGEQNELPPTTPKASLHPKKVMLCIWWDWKGVLYYELLPENQTINSNKYCSQLDQLKAALDEKRPELVNRERIIFHQDNARPHVSLMTRQKLLQLGWEVLIHPPYSPDIAPSDFHLFRSLQNSLNGKSFISLEDCKRHLDQFFAQKDKKFWEDGIMKLPEKWQKVVEQNGEYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CG27</accession>
    <name>A0A4W2CG27_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CG27"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021674.1">
        <property type="protein sequence ID" value="ENSBIXP00000011999.1"/>
        <property type="gene ID" value="ENSBIXG00000017143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="102"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="434" mass="49370" checksum="F712A517241C98DF" modified="2019-09-18" version="1">MQVHAKMGPINDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDQDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVEIFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNDRTVALISHASKVMLKIPQARLQQYVNRELPDIQAGFRKGRGTRDQIANIHWIIGKGREFQKKIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVKTGHGTIDWFQIGKGVCQGCILSPCLFNLYAEYFMRNAGLGEAQAGIKIAGRNVNNLRYADNTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGETVEAVSDFIFWGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRAITLPAKVHLVKPMVFPVVMYGCESWTVKKAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIJ5</accession>
    <name>A0A4W2CIJ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIJ5"/>
    <dbReference type="STRING" id="30522.A0A4W2CIJ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045253.1">
        <property type="protein sequence ID" value="ENSBIXP00000011735.1"/>
        <property type="gene ID" value="ENSBIXG00000016945.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF125">
        <property type="entry name" value="SIALIC ACID-BINDING IG-LIKE LECTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021393488" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="148"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="236"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="470"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="550" mass="60312" checksum="077D04E2F4806ABE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJK7</accession>
    <name>A0A4W2CJK7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nucleoside-triphosphate phosphatase</fullName>
            <ecNumber evidence="3">3.6.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ENTPD7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027384643.1">
        <property type="nucleotide sequence ID" value="XM_027528842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJK7"/>
    <dbReference type="STRING" id="30522.A0A4W2CJK7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003629.1">
        <property type="protein sequence ID" value="ENSBIXP00000006493.1"/>
        <property type="gene ID" value="ENSBIXG00000012612.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007632.1">
        <property type="protein sequence ID" value="ENSBIXP00005004873.1"/>
        <property type="gene ID" value="ENSBIXG00005010635.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884321"/>
    <dbReference type="CTD" id="57089"/>
    <dbReference type="GeneTree" id="ENSGT01150000286963"/>
    <dbReference type="OMA" id="HESIGFM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043273">
        <property type="term" value="F:CTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006254">
        <property type="term" value="P:CTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034656">
        <property type="term" value="P:nucleobase-containing small molecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072539">
        <property type="term" value="P:T-helper 17 cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046052">
        <property type="term" value="P:UTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd24045">
        <property type="entry name" value="ASKHA_NBD_NTPDase4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000057">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000003">
        <property type="entry name" value="ectonucleoside triphosphate diphosphohydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF37">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="552"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="261"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="604" mass="68847" checksum="3CB97AA07305A1DC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK81</accession>
    <name>A0A4W2CK81_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC113875757</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CK81"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045540.1">
        <property type="protein sequence ID" value="ENSBIXP00000012346.1"/>
        <property type="gene ID" value="ENSBIXG00000017444.1"/>
    </dbReference>
    <dbReference type="OMA" id="WMFSVLM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016503">
        <property type="term" value="F:pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000120">
        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="27"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="36577" checksum="90167C23DB8823AD" modified="2019-09-18" version="1">MSFHKDALRTTSQTALKTTYLIQIGVGSMANIILFFHNISPILLGHNRRPTPTVLAHISLANLLFLLSSGIPHIMTGFVLRNPLSSLGCKFVYYIQRVARSTALCSTCVLSTYQSFTLTPRRSEWVMLRGRAPKVTGPSCCTCWMFSLLMYIYVPVKITGPWDRYNNTDSQGKWFCSITGAATGFVYFWFISDVMFIGLMVWSSGSMVLLLHRHHQRVQYIHTPTGHHRCPPETRAAHTILMLMVTFVVFYLLNYSFVFHISASLDFRLWLLQVSNVLVSCFPTISPFLLLLRDPRTLFISLVFVIIWLCGIWGNSSWTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKB1</accession>
    <name>A0A4W2CKB1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CKB1"/>
    <dbReference type="STRING" id="30522.A0A4W2CKB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023984.1">
        <property type="protein sequence ID" value="ENSBIXP00000013521.1"/>
        <property type="gene ID" value="ENSBIXG00000006191.1"/>
    </dbReference>
    <dbReference type="OMA" id="CMVQVYA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15939">
        <property type="entry name" value="7tmA_OR4A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="39"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="309" mass="34558" checksum="A3DF8D457DA5F372" modified="2019-09-18" version="1">MANERNVTVFILVGLTQNPQMQKIVFVVFLVLYMVTLSGNLLIVVTITTSQALNSPMYFFLTHLSLIDTIYSSSSAPKLIVDSLQEIKTISFNGCMAQVYAEHIFGATEIILLTEMAYDHYVAICKPLHYTAIMSHRLCVLLLGVAWTGGFLHATIQVLFTVWLPFCGPNVIDHFMCDLYPLLKLVCVDTHILGLFVAANSGFICLLNFLLLLVSYVVILHSLRAYSLEGRHRALSTCVSHITVVVLSFVPCIFVYLCPVTTLPIDKAVAVFYTMVAPMLNPLIYTLRNAEVKNAMKKHWRKKVTADSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLA1</accession>
    <name>A0A4W2CLA1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-type tyrosine-protein phosphatase kappa</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">PTPRK</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022542.1">
        <property type="protein sequence ID" value="ENSBIXP00000012561.1"/>
        <property type="gene ID" value="ENSBIXG00000002992.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14636">
        <property type="entry name" value="R-PTPc-K-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000006">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000009">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000048">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000025">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF209">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021332295" description="Receptor-type tyrosine-protein phosphatase kappa" evidence="20">
        <location>
            <begin position="27"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="753"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="23">
        <location>
            <begin position="33"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="195"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="293"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="490"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="903"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1083"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="1195"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1371"/>
            <end position="1448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1461" mass="164548" checksum="8F209CDD457E653C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNE3</accession>
    <name>A0A4W2CNE3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ashwin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ashwin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CNE3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025677.1">
        <property type="protein sequence ID" value="ENSBIXP00000014675.1"/>
        <property type="gene ID" value="ENSBIXG00000019311.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024887">
        <property type="entry name" value="Ashwin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28359">
        <property type="entry name" value="ASHWIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28359:SF1">
        <property type="entry name" value="ASHWIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15323">
        <property type="entry name" value="Ashwin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="73"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="190" mass="21440" checksum="EACA31D3D310009B" modified="2019-09-18" version="1">MAGDVGGRSCTDSELLLHPELLSQEFLLLTLEQKNITVENDMRVNKDSLTDLYVQHAIPLPQRDLPKSRWGKMMEKKREQHEIKKETKRSSPADGLRKRPLIVFDGSSTSTTIKVKKTENGDNDRLRPQPQNHDLTHRKSPLGPVRSPPLSPVGATPVKLKRAAPKEEAEASINLKPPEAKRKIQHVTWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CP22</accession>
    <name>A0A4W2CP22_BOBOX</name>
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        <recommendedName>
            <fullName evidence="12">ATP synthase subunit</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CP22"/>
    <dbReference type="STRING" id="30522.A0A4W2CP22"/>
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        <property type="gene ID" value="ENSBIXG00000006405.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9175" checksum="2C3C9ED736989386" modified="2019-09-18" version="1">MAQFVRNLAEKALALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPTAIQSLKKIINSAKTGSFKHTLATSCEELTHWKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQF7</accession>
    <name>A0A4W2CQF7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQF7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016280.1">
        <property type="protein sequence ID" value="ENSBIXP00000008551.1"/>
        <property type="gene ID" value="ENSBIXG00000001701.1"/>
    </dbReference>
    <dbReference type="OMA" id="VAHITCT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:zymogen activation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000004">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
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        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021460617" description="SRCR domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="726"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="28"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="134"/>
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        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="239"/>
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        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="376"/>
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        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
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            <begin position="481"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="600"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="787"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="860"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="97"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="172"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="203"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="265"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="401"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="445"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="669"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="899" mass="96054" checksum="E43312C3E7CAC3ED" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQQ6</accession>
    <name>A0A4W2CQQ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acid phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQQ6"/>
    <dbReference type="STRING" id="30522.A0A4W2CQQ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024960.1">
        <property type="protein sequence ID" value="ENSBIXP00000014189.1"/>
        <property type="gene ID" value="ENSBIXG00000018935.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010206.1">
        <property type="protein sequence ID" value="ENSBIXP00005003496.1"/>
        <property type="gene ID" value="ENSBIXG00005009627.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158351"/>
    <dbReference type="OMA" id="IMHELTL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF34">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="6">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="121" mass="13561" checksum="CC158C3542073020" modified="2019-09-18" version="1">MAEQVTKSVLFVCLGNICQSPIAEAVFRKLVTDQNISDNWRIDSAATSTYELGNPPDYRGQACMKKHGIPMSHVARQSPYVLPFEPVCLWKCSYIQEKGECEGLSYGQLGDCVCFYMRIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSG8</accession>
    <name>A0A4W2CSG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-AMP-activated protein kinase subunit beta-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PRKAB1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027422548.1">
        <property type="nucleotide sequence ID" value="XM_027566747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSG8"/>
    <dbReference type="SMR" id="A0A4W2CSG8"/>
    <dbReference type="STRING" id="30522.A0A4W2CSG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017313.1">
        <property type="protein sequence ID" value="ENSBIXP00000009206.1"/>
        <property type="gene ID" value="ENSBIXG00000007438.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033388.1">
        <property type="protein sequence ID" value="ENSBIXP00005020158.1"/>
        <property type="gene ID" value="ENSBIXG00005023298.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907507"/>
    <dbReference type="CTD" id="5564"/>
    <dbReference type="GeneTree" id="ENSGT00940000155307"/>
    <dbReference type="OMA" id="QRTINAP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031669">
        <property type="term" value="P:cellular response to nutrient levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035878">
        <property type="term" value="P:nail development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000139">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="11">
        <location>
            <begin position="180"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="270" mass="30287" checksum="0ADEE97863AF8680" modified="2019-09-18" version="1">MGNTSSERAALDRQGGHKTPRRDSSGGSKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPVVTSQLGTVNNVIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYISKPEERFKAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTB2</accession>
    <name>A0A4W2CTB2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">lysozyme</fullName>
            <ecNumber evidence="4">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113892975</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 22 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTB2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047517.1">
        <property type="protein sequence ID" value="ENSBIXP00000016517.1"/>
        <property type="gene ID" value="ENSBIXG00000020682.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16897">
        <property type="entry name" value="LYZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000001">
        <property type="entry name" value="Lysozyme C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019799">
        <property type="entry name" value="Glyco_hydro_22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000974">
        <property type="entry name" value="Glyco_hydro_22_lys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF28">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00137">
        <property type="entry name" value="LYSOZYME"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00135">
        <property type="entry name" value="LYZLACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00263">
        <property type="entry name" value="LYZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00128">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021187812" description="lysozyme" evidence="12">
        <location>
            <begin position="38"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 22 (GH22)" evidence="13">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="166" mass="17841" checksum="B65AF57E853C5E7B" modified="2019-09-18" version="1">MLNSKSSSPGQLGHLASVNMKALLILGLLLLSVAVQGKTFERCELAKTLKNLGLAGYKGISLANWMCLAKVESGYNTQAIKYSSGGKSTDYGIFQINSKWWCNDGKTPKAVNGCGVSCSALLKDDITQAVACAKKIVSQQGITAWVAWKNKCRNRDVSSYIQGCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTM8</accession>
    <name>A0A4W2CTM8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nuclear valosin-containing protein-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NVL</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Participates in the assembly of the telomerase holoenzyme and effecting of telomerase activity via its interaction with TERT. Involved in both early and late stages of the pre-rRNA processing pathways. Spatiotemporally regulates 60S ribosomal subunit biogenesis in the nucleolus. Catalyzes the release of specific assembly factors, such as WDR74, from pre-60S ribosomal particles through the ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with NCL/nucleolin. Isoform 1 and isoform 2 interact with TERT and isoform 1 exhibits a higher binding affinity for TERT compared to isoform 2. Isoform 1 interacts with MTREX in an ATP-dependent manner; the interaction is required to associate NVL with nuclear RNA exosome. Isoform 1 interacts with RPL5 in an ATP-dependent manner. Interacts with WDR74 (through WDR repeats); the interaction is independent of RNA or pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTM8"/>
    <dbReference type="STRING" id="30522.A0A4W2CTM8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047585.1">
        <property type="protein sequence ID" value="ENSBIXP00000016673.1"/>
        <property type="gene ID" value="ENSBIXG00000020791.1"/>
    </dbReference>
    <dbReference type="OMA" id="GLWSTHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2010:FF:000001">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000063">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000172">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000600">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038100">
        <property type="entry name" value="NLV2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031996">
        <property type="entry name" value="NVL2_nucleolin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16725">
        <property type="entry name" value="Nucleolin_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="296"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="613"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="132"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="496"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="855" mass="94624" checksum="4778CF8BD8AA4858" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZ80</accession>
    <name>A0A4W2CZ80_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHC class I-like antigen recognition-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZ80"/>
    <dbReference type="STRING" id="30522.A0A4W2CZ80"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047877.1">
        <property type="protein sequence ID" value="ENSBIXP00000017261.1"/>
        <property type="gene ID" value="ENSBIXG00000021263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002486">
        <property type="term" value="P:antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002476">
        <property type="term" value="P:antigen processing and presentation of endogenous peptide antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000004">
        <property type="entry name" value="Retinoic acid early-inducible protein 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF1">
        <property type="entry name" value="UL16 BINDING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021194513" description="MHC class I-like antigen recognition-like domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="MHC class I-like antigen recognition-like" evidence="7">
        <location>
            <begin position="26"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="242" mass="27515" checksum="77B3C2BA1EB79BF0" modified="2019-09-18" version="1">MGGFKTSLGFLVLLPIVLFSGTSSDAHSLSYNVTIDPRPRDGQPWCEVQGEVDQKVFLSYDCGRAKIMYMSPLGEEVKSMNSWETQTDTLRDIGDLLKEQMPDVTPEKHIDEGPLTLQARMTCWREDNGHTSASWEFGFNGQLCLLFDSENGYWTMVHSKGRRMKEKWENDRAVMDFFKKVSMGDCQRWYHTSLVRWEKMLKTAASPTEAPLRVNSTATATKHITCILPVLLSSFIIIVFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0I4</accession>
    <name>A0A4W2D0I4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D0I4"/>
    <dbReference type="STRING" id="30522.A0A4W2D0I4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032769.1">
        <property type="protein sequence ID" value="ENSBIXP00000019245.1"/>
        <property type="gene ID" value="ENSBIXG00000022911.1"/>
    </dbReference>
    <dbReference type="OMA" id="VTQPDAY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF24">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 8-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="112" mass="12536" checksum="7BA184EFBD3E4475" modified="2019-09-18" version="1">AQLVTQPDGHINVSEGNHVELRCNYSYGGSIYLFWYVQYPNQGLQFLLKYLPGPTRVKGIKGFEADFKKDENSFHLMKASAHWSDSAKYFCALCDTVPESAGGAEQKPSQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4M8</accession>
    <name>A0A4W2D4M8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thiolase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4M8"/>
    <dbReference type="STRING" id="30522.A0A4W2D4M8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024188.1">
        <property type="protein sequence ID" value="ENSBIXP00000013663.1"/>
        <property type="gene ID" value="ENSBIXG00000018561.1"/>
    </dbReference>
    <dbReference type="OMA" id="YVRVETI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="6">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="19498" checksum="D628B15222D1C829" modified="2019-09-18" version="1">MEAMSKLKPCFLMDGTGTVTPANASGMNDGAAAVVLMKTSEAGNCRLTPLAQIVSWAQADVEPSIMGIGPIPAIKQAVAKAGWSLEDVDVFEINEAFAALSVAIAKELGLNPEQVNTEGGAIALGHPLGASVCSILVTLLHTLEHKGGHCGVAAYVRVETISHFTRNARLDINYVGNYLGEKNRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5E1</accession>
    <name>A0A4W2D5E1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">2-oxoglutarate dehydrogenase-like, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">2-oxoglutarate dehydrogenase complex component E1-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Alpha-ketoglutarate dehydrogenase-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">2-oxoglutarate dehydrogenase (E1-like) component of the 2-oxoglutarate dehydrogenase multienzyme complex (OGDHC) which mediates the decarboxylation of alpha-ketoglutarate in the tricarboxylic acid cycle. The OGDHC complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) while reducing NAD(+) to NADH. The OGDHC complex is mainly active in the mitochondrion. Involved in the inhibition of cell proliferation and in apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">The OGDHC complex comprises multiple copies of three catalytic enzyme components, the 2-oxoglutarate dehydrogenase (OGDH/E1), the dihydrolipoamide dehydrogenase (DLST/E2) and the dihydrolipoamide dehydrogenase (DLD/E3). OGDHL/E1-like isoenzyme may replace OGDH in the OGDHC complex in the brain. The presence of either ODGH/E1 or ODGHL/E1-like isoenzyme in the complex may depend on its tissular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5E1"/>
    <dbReference type="STRING" id="30522.A0A4W2D5E1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033025.1">
        <property type="protein sequence ID" value="ENSBIXP00000019444.1"/>
        <property type="gene ID" value="ENSBIXG00000023065.1"/>
    </dbReference>
    <dbReference type="OMA" id="IRIRRHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF5">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="20">
        <location>
            <begin position="749"/>
            <end position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1121" mass="126368" checksum="C1A953A4AEB9CED7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5H7</accession>
    <name>A0A4W2D5H7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5H7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048961.1">
        <property type="protein sequence ID" value="ENSBIXP00000019473.1"/>
        <property type="gene ID" value="ENSBIXG00000023080.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_fold"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18647" checksum="577A0354DB813668" modified="2019-09-18" version="1">MLSRSVVPHSVRPQRQQPTRLLCPWDFPGKRGAGAQSVTQCNYSSPVSPYLFWYVQYPKQGLQLLLKYISGEGLISGIKGFKAEFKSGATFHMEKPLAHWRDSATYFCALGDTVCGTERGAEHKLLVNMRLSVTQEIDLGHFQKDRIFYRHVGQRELNLALMQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5V5</accession>
    <name>A0A4W2D5V5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Fibroblast growth factor receptor</fullName>
            <ecNumber evidence="25">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">FGFR4</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Monomer. Homodimer after ligand binding. Interacts with FGF1, FGF2, FGF4, FGF6, FGF8, FGF9, FGF16, FGF17, FGF18, FGF19, FGF21 and FGF23 (in vitro). Binding affinity for FGF family members is enhanced by interactions between FGFs and heparan sulfate proteoglycans. Interacts with KLB; this strongly increases the affinity for FGF19 and FGF23. Affinity for FGF19 is strongly increased by KLB and sulfated glycosaminoglycans. KLB and KL both interact with the core-glycosylated FGFR4 in the endoplasmic reticulum and promote its degradation, so that only FGFR4 with fully mature N-glycans is expressed at the cell surface. Identified in a complex with NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with MMP14 and HIP1. Interacts with STAT3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="25"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5V5"/>
    <dbReference type="SMR" id="A0A4W2D5V5"/>
    <dbReference type="STRING" id="30522.A0A4W2D5V5"/>
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        <property type="protein sequence ID" value="ENSBIXP00000021273.1"/>
        <property type="gene ID" value="ENSBIXG00000004919.1"/>
    </dbReference>
    <dbReference type="OMA" id="CHGMANC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070857">
        <property type="term" value="P:regulation of bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05857">
        <property type="entry name" value="IgI_2_FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000007">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000020">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000730">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000334">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016248">
        <property type="entry name" value="FGF_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF343">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000628">
        <property type="entry name" value="FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19 28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="17 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 30" id="KW-0732">Signal</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021205708" description="Fibroblast growth factor receptor" evidence="30">
        <location>
            <begin position="20"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="32">
        <location>
            <begin position="151"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="32">
        <location>
            <begin position="248"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="466"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="611"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="472"/>
            <end position="478"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27 29">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="550"/>
            <end position="552"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="615"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="57"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="171"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="270"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00063358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRNR" id="PIRNR000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="800" mass="88263" checksum="E74D17093B17F663" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D752</accession>
    <name>A0A4W2D752_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Homeobox A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HOXA1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Antp homeobox family. Labial subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D752"/>
    <dbReference type="SMR" id="A0A4W2D752"/>
    <dbReference type="STRING" id="30522.A0A4W2D752"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025492.1">
        <property type="protein sequence ID" value="ENSBIXP00000014561.1"/>
        <property type="gene ID" value="ENSBIXG00000019217.1"/>
    </dbReference>
    <dbReference type="OMA" id="SAQTFDW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="227"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="279"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="333" mass="36255" checksum="833BD6851DCE5EF9" modified="2019-09-18" version="1">MDNARMSSFLEYPILSGGDSGTCSARAYPSDHGITTFQSCAVSANSCGSDDRFLVGRGVQISPPHHHHHHHHPQPTTYQTSGNLGVSYSHSSCGPSYSAQNFGAPYSPYALNQEAEVSGGYPSCAPAVYSGNLSSPMVQHHHHHQGYAGGAVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKVKRNPPKTGKIGEYGYVGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQKKREKEGLLPISPATPTGSEEKAEESSEKSSSSPCVPSPGSSTSDTLPTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7B9</accession>
    <name>A0A4W2D7B9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7B9"/>
    <dbReference type="SMR" id="A0A4W2D7B9"/>
    <dbReference type="STRING" id="30522.A0A4W2D7B9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025595.1">
        <property type="protein sequence ID" value="ENSBIXP00000014626.1"/>
        <property type="gene ID" value="ENSBIXG00000019255.1"/>
    </dbReference>
    <dbReference type="OMA" id="SENAYIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000005">
        <property type="entry name" value="ataxin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033865">
        <property type="entry name" value="Ataxin-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF0">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="12">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="151" mass="17708" checksum="3EB4069D6C8081FB" modified="2019-09-18" version="1">MESIFHEKQEGSLCAQHCLNNLLQGEYFIPVELSSIAHQLHEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVTSNALKVWGLELILFNSPEYQRLRINPINERSFICNYKEHWFTVRKLGKQKVTLYLLLRVICQIAKLTNFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D848</accession>
    <name>A0A4W2D848_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113891792</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D848"/>
    <dbReference type="STRING" id="30522.A0A4W2D848"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049394.1">
        <property type="protein sequence ID" value="ENSBIXP00000020401.1"/>
        <property type="gene ID" value="ENSBIXG00000004712.1"/>
    </dbReference>
    <dbReference type="OMA" id="VISYMCI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15420">
        <property type="entry name" value="7tmA_OR2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="310" mass="34561" checksum="7464C05A07B95E6E" modified="2019-09-18" version="1">MGDNMSSVTEFILLGFPLNTRTQMLLFGLFSLFYVLTLLGNGVILGLISLDPRLHTPMYFFLSHLAIADMAYACSTVPQMLANLLSPAKPISFAGCITQTFLFLTFAITECLLLMVMSYDRYVAICHPLRYSVIMSWTVCVMLVVTSWTIGVLLSLVHLVLLMPLPFCMAQKIDHFFCEIMAVLKLACADTHINELMVLAGAVSVLVGPFSSVIISYVCIFRAILRLQSGEGQSKAFSTCSSHLCVVGLFYGTAIIMYVEPRHGNPKEQKKYLLLFHSLFNPMLNPLIYTLKNSEVKNAMKRVNPRADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9Z9</accession>
    <name>A0A4W2D9Z9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ras homolog family member J</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RHOJ</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410072.1">
        <property type="nucleotide sequence ID" value="XM_027554271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9Z9"/>
    <dbReference type="STRING" id="30522.A0A4W2D9Z9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035400.1">
        <property type="protein sequence ID" value="ENSBIXP00000021002.1"/>
        <property type="gene ID" value="ENSBIXG00000024197.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011552.1">
        <property type="protein sequence ID" value="ENSBIXP00005029193.1"/>
        <property type="gene ID" value="ENSBIXG00005009084.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900476"/>
    <dbReference type="CTD" id="57381"/>
    <dbReference type="GeneTree" id="ENSGT00940000159098"/>
    <dbReference type="OMA" id="ARMNCKE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090050">
        <property type="term" value="P:positive regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903670">
        <property type="term" value="P:regulation of sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061299">
        <property type="term" value="P:retina vasculature morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000438">
        <property type="entry name" value="Rho-related GTP-binding protein RhoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="236" mass="25858" checksum="C4009F4D264457AF" modified="2019-09-18" version="1">MGFGRSGRSNNHSTVTAAGAASMNCQEGNDSSCGCGGHEEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKGCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKHCSQCHSCCSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA41</accession>
    <name>A0A4W2DA41_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA41"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038144.1">
        <property type="protein sequence ID" value="ENSBIXP00000022848.1"/>
        <property type="gene ID" value="ENSBIXG00000025622.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013938.1">
        <property type="protein sequence ID" value="ENSBIXP00005001596.1"/>
        <property type="gene ID" value="ENSBIXG00005008067.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="454" mass="52132" checksum="21771B60C089438E" modified="2019-09-18" version="1">MPAHLENSAVATGLEKVSFHSNPKECSNYCTIALISHASKIMLKILQARLQQYVNRELPAVQAGFRKGRGARDQIANIRWIMEKAREFQKNIYFCFIDYVKAFDCVDHNKLWKILKEMGIPDHLTCFLRNLYAGQDATVRTGHGTTDWFQIGKGVRQGCMLSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNINNLRYTDDTTLMAESEEELKSLLLMEVKEESEKVGLKLNIQKTKIMASGPTTSWEIDGETVETVSDFILGGSKITADGDCSHETKRRLLLGRKVMTNLDSIFKRRDMTLPTKVHLVEAMVFPVIMYGCESWNVKKAEHRRIDPFELWCWRRLLRVPWTARRSNQCNLKEISPGCSLKGLMLKLKLQYFGHLMQRVDSLEKTLMLGGIGGRRRRGRQRMRWLDGITSSMDVSLSELWELVMDREAWHAAIHGVTRSWTWLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDX4</accession>
    <name>A0A4W2DDX4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DDX4"/>
    <dbReference type="STRING" id="30522.A0A4W2DDX4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051523.1">
        <property type="protein sequence ID" value="ENSBIXP00000024341.1"/>
        <property type="gene ID" value="ENSBIXG00000026539.1"/>
    </dbReference>
    <dbReference type="OMA" id="EHTYYSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF18">
        <property type="entry name" value="OVOCHYMASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021350183" evidence="8">
        <location>
            <begin position="29"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="53"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="386"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="9">
        <location>
            <begin position="659"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="896" mass="99620" checksum="A679DDC504AB82B1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFZ4</accession>
    <name>A0A4W2DFZ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Periodic tryptophan protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PWP1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Chromatin-associated factor that regulates transcription. Regulates Pol I-mediated rRNA biogenesis and, probably, Pol III-mediated transcription. Regulates the epigenetic status of rDNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Associates with the RNA polymerase (Pol I) complex. Interacts with POLR1E.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the WD repeat PWP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397675.1">
        <property type="nucleotide sequence ID" value="XM_027541874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFZ4"/>
    <dbReference type="SMR" id="A0A4W2DFZ4"/>
    <dbReference type="STRING" id="30522.A0A4W2DFZ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051962.1">
        <property type="protein sequence ID" value="ENSBIXP00000025051.1"/>
        <property type="gene ID" value="ENSBIXG00000027175.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033874.1">
        <property type="protein sequence ID" value="ENSBIXP00005040795.1"/>
        <property type="gene ID" value="ENSBIXG00005023573.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892691"/>
    <dbReference type="CTD" id="11137"/>
    <dbReference type="GeneTree" id="ENSGT00390000017324"/>
    <dbReference type="OMA" id="CFVPRGV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140713">
        <property type="term" value="F:histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990889">
        <property type="term" value="F:histone H4K20me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000738">
        <property type="term" value="P:positive regulation of stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901838">
        <property type="term" value="P:positive regulation of transcription of nucleolar large rRNA by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000241">
        <property type="entry name" value="periodic tryptophan protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="251"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="294"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="380"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="38"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="500" mass="55865" checksum="55CF4CFE17F15DA3" modified="2019-09-18" version="1">MNRSRQVTCVAWVRCGVAKETPDKVELSKEEVKRLIAEAKEKLQEEGGSDEQETGNPSEDGMQSARTQARPREPLEDGDPEDDRTLDDDELAEYDLDKYDEEGDPDAETLGESLLGLTVYGSNDQDPYVTLKDTEQYEREDFLIKPSDNLIVCGRAEQDQCNLEVHVYNQEEDSFYVHHDILLSAYPLSVEWLNFDPSPDDSTGNYIAVGNMTPVIEVWDLDIVDSLEPVFTLGSKLSKKKKKKGKKNSSADGHTDAVLDLSWNKLVRNVLASASADNTVILWDMSLGKPAASLAVHTDKVQTLQFHPFEAQTLISGSYDKSVALYDCRSPEESHRMWRFSGQIERVTWNHFSPCHFLASTDDGFVYNLDARSDKPIFTLNAHNDEISGLDLSSQIKGCLVTASADKYVKIWDILGDRPSLVHSRDMKMGVLFCSSCCPDLPFIYAFGGQKEGLRVWDISTVSSVNEAFGRRERLVLGNARTSSISGPFGNRSSETPMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGA6</accession>
    <name>A0A4W2DGA6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solute carrier family 25 member 48</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGA6"/>
    <dbReference type="SMR" id="A0A4W2DGA6"/>
    <dbReference type="STRING" id="30522.A0A4W2DGA6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038922.1">
        <property type="protein sequence ID" value="ENSBIXP00000023367.1"/>
        <property type="gene ID" value="ENSBIXG00000025865.1"/>
    </dbReference>
    <dbReference type="OMA" id="VWFLAFE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000058">
        <property type="entry name" value="Solute carrier family 25 member 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF7">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="116"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="230"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="327" mass="34853" checksum="6AF53A758A890887" modified="2019-09-18" version="1">MGTDPAPGKRGAAAGAMGNFQLEDFVAGWIGGAASVIVGHPLDTVKARLQAGSGYGSTLSCIRTVYRRESVFGFFKGMSFPLASIAVYNSVVFGVFSNTQRFLSHHRCQEPEAGPPHVLSDLLLASMVAGVVSVGLGAPVDLIKIRLQMQTQPFQEANLGLKPRVAALGEQPAYQGPVHCFATIVRTEGLAGLYRGASAMLLRDVPGYCLYFIPYVFLSDWITPEACAGPSPCAVWLAGGMAGAISWGTATPMDVVKSRLQADGVYVNKYRGVLDCMSQSYQKEGLKVFFRGITVNAVRGFPMSAAMFLGYELSLQAIRGDHVVTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGD2</accession>
    <name>A0A4W2DGD2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D(3) dopamine receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Dopamine D3 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CLIC6. Interacts with GRK4. Interacts with PALM. Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGD2"/>
    <dbReference type="STRING" id="30522.A0A4W2DGD2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030609.1">
        <property type="protein sequence ID" value="ENSBIXP00000017885.1"/>
        <property type="gene ID" value="ENSBIXG00000021794.1"/>
    </dbReference>
    <dbReference type="OMA" id="SNGRMMT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007195">
        <property type="term" value="P:adenylate cyclase-inhibiting dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048148">
        <property type="term" value="P:behavioral response to cocaine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051481">
        <property type="term" value="P:negative regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045744">
        <property type="term" value="P:negative regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051967">
        <property type="term" value="P:negative regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060158">
        <property type="term" value="P:phospholipase C-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014059">
        <property type="term" value="P:regulation of dopamine secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15310">
        <property type="entry name" value="7tmA_D3_dopamine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000287">
        <property type="entry name" value="Dopamine receptor D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001620">
        <property type="entry name" value="Dopamine_D3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF154">
        <property type="entry name" value="D(3) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00568">
        <property type="entry name" value="DOPAMINED3R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="78"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="479" mass="53090" checksum="78A8736F27CF76AD" modified="2019-09-18" version="1">MVTGAEVLNPTASRNQKKFSGKLVGVTRSLGAMAPLNQLGGHINATCAAENSTGAGRARPHAYYALSYCALILAIVFGNVLVCVAVLKERALQTTTNYLVVSLAVADLLVATLVMPWVVYLEVTGGVWNFSRVCCDVFVTLDVMMCTASILNLCAISIDRYTAVVMPVHYQQGTGQSSCRRVALMITAVWLLSFAVSCPLLFGFNTTGDPSICSISNPDFVLYSSVVSFYLPFGVTVLVYARIYVVLRQRRRKRILTRQNSQCLSVRPSFPQQSSCLRLHPIRQFSIRARFPSDATGKMEHSEDKQYPQKCQDPLLLHLQPLSPGQTHMELKRYYSICQDTALGTPGFQEGEGDLKREGRTRNSLSPTMAPKLSLEVRKLSNGRLSTSLRLGPLQPRAVPLREKKATQMLVIVLGTFIVCWLPFFLTHVLNTHCRACHVSPQLYSATTWLGYVNSALNPVIYTTFNIEFRKAFLKILSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKE8</accession>
    <name>A0A4W2DKE8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKE8"/>
    <dbReference type="STRING" id="30522.A0A4W2DKE8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048936.1">
        <property type="protein sequence ID" value="ENSBIXP00000019383.1"/>
        <property type="gene ID" value="ENSBIXG00000023009.1"/>
    </dbReference>
    <dbReference type="OMA" id="KNESHYT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF132">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 26-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021488585" description="Ig-like domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="130" mass="15070" checksum="5B3FFDE23FB980A5" modified="2019-09-18" version="1">MHLIHRITLIWTVTDAKTTQPSSMDYPEGQDVTLPCNHSTIGRNEYIYWYQQNPNQSPQYVIHGLRGTVNSSTASLTIASDRKSSTLVLPQVTLRDTAVYYCVLREAHWDRREFGELWNTKNRNCNIEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKH2</accession>
    <name>A0A4W2DKH2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Apolipoprotein L3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC113892663</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397590.1">
        <property type="nucleotide sequence ID" value="XM_027541789.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397591.1">
        <property type="nucleotide sequence ID" value="XM_027541790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKH2"/>
    <dbReference type="STRING" id="30522.A0A4W2DKH2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041164.1">
        <property type="protein sequence ID" value="ENSBIXP00000024807.1"/>
        <property type="gene ID" value="ENSBIXG00000026945.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036770.1">
        <property type="protein sequence ID" value="ENSBIXP00005042055.1"/>
        <property type="gene ID" value="ENSBIXG00005004311.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892663"/>
    <dbReference type="GeneTree" id="ENSGT01030000234599"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF27">
        <property type="entry name" value="APOLIPOPROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="347" mass="38675" checksum="50E74554D98B5A3D" modified="2019-09-18" version="1">MSSENFRHCSDIETFFEEVVQCLWNILSREELLLLLNEFLERIKAEASLSREDVDELHKYLNELKRALVEEDQERLSKEQLDRRRFLNQFPRVKRQLEECISKFRELADKAEKVHKGCTISNVMAHSTGAVSGILTIVGLALALMTMGSSVVLLATVTGLGAVGTVTSVSTSILEHMKRSSVETIASRMMSTLIKKWKVLLEILKSNPTTEKVTKAVQCIEMHIHDMQTGKAISDSAANILMGLMILSSPSIQKKEAGFKAPAFTVTKGGQIVGLATSGVFLLVDVGFLVKDSKHLHDGAKAASAENLWQRARELERKLEELTQIYERLQEDLIQPPPEQCGVQGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLB0</accession>
    <name>A0A4W2DLB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052006.1">
        <property type="protein sequence ID" value="ENSBIXP00000025124.1"/>
        <property type="gene ID" value="ENSBIXG00000027243.1"/>
    </dbReference>
    <dbReference type="OMA" id="CALMAYQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="75"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="363" mass="41538" checksum="01437A9BA04C76C5" modified="2019-09-18" version="1">MARIHRRTVQKDLHDPDNHDGVITDLEPDILECEVKWALESITMNKASGGDRIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQSRLQQYVNCELPDVQAGFRKGRGTIDQIANIRWIMEKAREFQKNIYFFFIDYATAFDCVHHHKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTNWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNVNHLSYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNVQKTKIMASGPITSWEIDGETVETCQTLFFWAPKSLQMVTAAMKLKDTYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLJ1</accession>
    <name>A0A4W2DLJ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TBXA2R</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLJ1"/>
    <dbReference type="SMR" id="A0A4W2DLJ1"/>
    <dbReference type="STRING" id="30522.A0A4W2DLJ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033497.1">
        <property type="protein sequence ID" value="ENSBIXP00000019751.1"/>
        <property type="gene ID" value="ENSBIXG00000023303.1"/>
    </dbReference>
    <dbReference type="OMA" id="HAILFDW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045777">
        <property type="term" value="P:positive regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045907">
        <property type="term" value="P:positive regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000163">
        <property type="entry name" value="Thromboxane A2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001105">
        <property type="entry name" value="Thbox_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF5">
        <property type="entry name" value="THROMBOXANE A2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00429">
        <property type="entry name" value="THROMBOXANER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="343" mass="37841" checksum="625FBA8C341250A5" modified="2019-09-18" version="1">MWPNASSLGPCFRPMNITLEERRLIASPWFAASFCLVGLASNLLALSVLMGARQGSSQSRSSFLTFLCGLVLTDFMGLLVTGAIVVTQHFVLFEWQAVDPGCSLCHFMGVIMVFFGLCPLLLGAAMASERFLGITRPFSRPATASQRRAWTTVGLVWASALALGLLPLLGVGHYTVQYPGSWCFLTLGTDPGDVAFGLLFALLGSISVGMSFLLNTISVATLCHVYHGQATAQQRPRDCEVEMMVQLMGIMVVASICWMPLLVFIAQTVLQSPPAMSPTGQLSRLTERQLLIYLRVATWNQILDPWVYILFRRAVIQRFYPRLSTRSRSLSLQPQLTRRSTIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQP9</accession>
    <name>A0A4W2DQP9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family. USP17 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQP9"/>
    <dbReference type="STRING" id="30522.A0A4W2DQP9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015437.1">
        <property type="protein sequence ID" value="ENSBIXP00000026806.1"/>
        <property type="gene ID" value="ENSBIXG00000029366.1"/>
    </dbReference>
    <dbReference type="OMA" id="HAGWICH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000070">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 17-like protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
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        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF651">
        <property type="entry name" value="INACTIVE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 17-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
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    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
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    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="77"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="379"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="518" mass="55676" checksum="71FB83433FF0894C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQV5</accession>
    <name>A0A4W2DQV5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC113905004</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQV5"/>
    <dbReference type="STRING" id="30522.A0A4W2DQV5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008871.1">
        <property type="protein sequence ID" value="ENSBIXP00000028896.1"/>
        <property type="gene ID" value="ENSBIXG00000010315.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF161">
        <property type="entry name" value="OLFACTORY RECEPTOR 52N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="227"/>
        </location>
    </feature>
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        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="43"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="35851" checksum="EC4CF64ED242A174" modified="2019-09-18" version="1">MLGANSSSLTPKFFILNGVPGLETAHIWISLPFCFMYIIAVVCNCGLIYLIGHEEALHHPMYYFLALLSFTDVTLCTTTVPNMLCIFWFNLKEIDFNGCLAQMFFVHTLTGMESGVLMLMALDRYVAICYPLRYATILTSAVIAKAGLATLLRGVMLIFPFTFLTKRLPYCQGNFIPHTYCDHMSVAKVSCGNFRVNAIYGLMVALLIGVFDMCCIAVSYTMILRAVVSLSSTDARHKAFSTCTSHICAIVITYVPAFFTFFTHRFGGHSVPHHIHIIVANLYLLLPPTMNPIVYGIKTKLIREGVIKFLLGEKVVFTQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRM3</accession>
    <name>A0A4W2DRM3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
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    <dbReference type="STRING" id="30522.A0A4W2DRM3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007957.1">
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    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005040358.1"/>
        <property type="gene ID" value="ENSBIXG00005023006.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000191094"/>
    <dbReference type="OMA" id="YSSPEMC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="77" mass="8669" checksum="222D764B3BDCF151" modified="2019-09-18" version="1">ESAACRHGSYRSLLASCYSSPEMCFVFSNRYKLQTWQQLWLWLAEARQTLADHSWEMLLSLDSGSGHGSPEPDACLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT30</accession>
    <name>A0A4W2DT30_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">KH domain-containing, RNA-binding, signal transduction-associated protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KHDRBS2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KHDRBS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027380385.1">
        <property type="nucleotide sequence ID" value="XM_027524584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT30"/>
    <dbReference type="STRING" id="30522.A0A4W2DT30"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006937.1">
        <property type="protein sequence ID" value="ENSBIXP00000029510.1"/>
        <property type="gene ID" value="ENSBIXG00000011159.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020899.1">
        <property type="protein sequence ID" value="ENSBIXP00005011825.1"/>
        <property type="gene ID" value="ENSBIXG00005016171.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881564"/>
    <dbReference type="CTD" id="202559"/>
    <dbReference type="GeneTree" id="ENSGT00940000157134"/>
    <dbReference type="OMA" id="YGHGVNE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22469">
        <property type="entry name" value="KH-I_KHDRBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000030">
        <property type="entry name" value="KH domain-containing, RNA-binding, signal transduction-associated protein 3 isoformX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032571">
        <property type="entry name" value="Qua1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF34">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16274">
        <property type="entry name" value="Qua1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="K Homology" evidence="12">
        <location>
            <begin position="57"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="178"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="347" mass="38651" checksum="AC7E20D8FA49DE8E" modified="2019-09-18" version="1">MEEKYLPELMAERDSLDPSFVHASRLLAEEIEKFQGSDGKSEDEEEKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKAKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFLVPDYNDEIRQEQLRELSYLNGSEDSGRGRGVRGRGIRIAPTAPSRGRGGAIPPPPPPGRGILTPRGTTVTRGALPVPPIARGVPTPRARGSPTMPGYRAPPLPAHKGYEAYGYDGYGGEYDDEIYETYDNSYPIQAQSVPEYYDYGHGVSEDAYDSYAPEEWATTHSSLKAPPSRSARGGYREHPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUH5</accession>
    <name>A0A4W2DUH5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inositol 1,4,5-trisphosphate receptor-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ITPRIP</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Enhances Ca(2+)-mediated inhibition of inositol 1,4,5-triphosphate receptor (ITPR) Ca(2+) release.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus outer membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ITPRIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384604.1">
        <property type="nucleotide sequence ID" value="XM_027528803.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384605.1">
        <property type="nucleotide sequence ID" value="XM_027528804.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384606.1">
        <property type="nucleotide sequence ID" value="XM_027528805.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384607.1">
        <property type="nucleotide sequence ID" value="XM_027528806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUH5"/>
    <dbReference type="STRING" id="30522.A0A4W2DUH5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005285.1">
        <property type="protein sequence ID" value="ENSBIXP00000030028.1"/>
        <property type="gene ID" value="ENSBIXG00000001009.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017975.1">
        <property type="protein sequence ID" value="ENSBIXP00005033800.1"/>
        <property type="gene ID" value="ENSBIXG00005002045.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017988.1">
        <property type="protein sequence ID" value="ENSBIXP00005033804.1"/>
        <property type="gene ID" value="ENSBIXG00005002045.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044891.1">
        <property type="protein sequence ID" value="ENSBIXP00005009840.1"/>
        <property type="gene ID" value="ENSBIXG00005002045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884308"/>
    <dbReference type="CTD" id="85450"/>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="OMA" id="CHLHCLQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008625">
        <property type="term" value="P:extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000006">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026250">
        <property type="entry name" value="ITPRIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF8">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02107">
        <property type="entry name" value="INOS145TPRIP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610890" description="Inositol 1,4,5-trisphosphate receptor-interacting protein" evidence="15">
        <location>
            <begin position="16"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="16">
        <location>
            <begin position="430"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="38"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="556" mass="63432" checksum="01A0AB9C31B808CC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DW88</accession>
    <name>A0A4W2DW88_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">S100A5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
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    </dbReference>
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        <property type="entry name" value="ADAMTS-like protein 3 isoform A"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF169">
        <property type="entry name" value="ADAMTS-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610920" evidence="10">
        <location>
            <begin position="27"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="897"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1186"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1285"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1139"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1158"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="102"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1639" mass="182302" checksum="A9A513A4E551BC29" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZN4</accession>
    <name>A0A4W2DZN4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">InaD-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Channel-interacting PDZ domain-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Pals1-associated tight junction protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein associated to tight junctions</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PATJ</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Scaffolding protein that facilitates the localization of proteins to the cell membrane. Required for the correct formation of tight junctions and epithelial apico-basal polarity. Acts (via its L27 domain) as an apical connector and elongation factor for multistranded TJP1/ZO1 condensates that form a tight junction belt, thereby required for the formation of the tight junction-mediated cell barrier. Positively regulates epithelial cell microtubule elongation and cell migration, possibly via facilitating localization of PRKCI/aPKC and PAR3D/PAR3 at the leading edge of migrating cells. Plays a role in the correct reorientation of the microtubule-organizing center during epithelial migration. May regulate the surface expression and/or function of ASIC3 in sensory neurons. May recruit ARHGEF18 to apical cell-cell boundaries.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Apical cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000051812.1">
        <property type="protein sequence ID" value="ENSBIXP00000024801.1"/>
        <property type="gene ID" value="ENSBIXG00000026955.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06673">
        <property type="entry name" value="PDZ10_MUPP1-PDZ8_PATJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06674">
        <property type="entry name" value="PDZ11_MUPP1-PDZ9_PATJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06675">
        <property type="entry name" value="PDZ12_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06676">
        <property type="entry name" value="PDZ13_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06689">
        <property type="entry name" value="PDZ1_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06667">
        <property type="entry name" value="PDZ2_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06791">
        <property type="entry name" value="PDZ3_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06668">
        <property type="entry name" value="PDZ4_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06669">
        <property type="entry name" value="PDZ5_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06671">
        <property type="entry name" value="PDZ7_MUPP1-PD6_PATJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06672">
        <property type="entry name" value="PDZ8_MUPP1-PDZ7_PATJ-PDZ2_INAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000112">
        <property type="entry name" value="inaD-like protein isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000070">
        <property type="entry name" value="Multiple PDZ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000038">
        <property type="entry name" value="Multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000051">
        <property type="entry name" value="Multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000054">
        <property type="entry name" value="multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000058">
        <property type="entry name" value="multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.360:FF:000001">
        <property type="entry name" value="PATJ, crumbs cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000125">
        <property type="entry name" value="PATJ, crumbs cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015132">
        <property type="entry name" value="L27_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051342">
        <property type="entry name" value="PDZ_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964:SF11">
        <property type="entry name" value="INAD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964">
        <property type="entry name" value="MULTIPLE PDZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09045">
        <property type="entry name" value="L27_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="L27" evidence="19">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="134"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="248"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="366"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="553"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="686"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="1069"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="1240"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="1437"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="1533"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="1676"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="1799"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="955"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1167"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1331"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1618"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1656"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="485"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="955"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1196"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1211"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1368"/>
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        <location>
            <begin position="1618"/>
            <end position="1641"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00037831"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00056700"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00071162"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00076734"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00076934"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00081298"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024801.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1882" mass="204590" checksum="40E678BAC669EDE1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0M0</accession>
    <name>A0A4W2E0M0_BOBOX</name>
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            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="117"/>
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            <dbReference type="PROSITE" id="PS50878"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025138.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="500" mass="57737" checksum="3FE6B985E4745625" modified="2019-09-18" version="1">MGKTRDLFEKIRDTKGTFHAKMSLIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHHGVITDLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSDYRTIALTSHASKVMLKILQARLQQYVNRELPDVQAGFRKRRGTRDQIANIRWIMEKAREFQKNIYFCFIDYVKAFDCVDHKKLWEILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEEAQAGIKIAGGNINNLRYADDTTLMAESEESLLMKVKEESEKVDLKLHIQKTKIMASGPTTSWEIDAETVETGSDFMFLGSKITTAGDCIHEIKRRLLLGRKVMTNLDGIFKSKDITLPTKVHLVKAMVFPVVMCGCESWTVKKAERRRIDAFELWCWRRLWRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E168</accession>
    <name>A0A4W2E168_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000029842.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="160"/>
            <end position="416"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029842.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="416" mass="47657" checksum="9CF9F3A7F02017D6" modified="2019-09-18" version="1">MSIESVMPSSHLILWRPLILLPSIPPSIRVFSNESTLCMRWPKYWNFSFSIIPSKEIPGLISFRMDWLDLLAVQGTLKSLLQDHSSKASILWHSAFFTVQLSHPYMTTGKTIALTRRIFVGKVMSLLLNMLSKLVITFLPRSKCLLISWLQSLSSVILEPRKIKSDTVSTVSPSIYHEVNAKECSNYHTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANICWIVEKAREFQKNIYFCFIDYAKAFDCVDYNKLWKILKEMGIPDHLICLLRTLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPWLFNLYAEYIMRNARLEETQAGIKIAGRNINHLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGTITSWETNYTLLKNLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3E9</accession>
    <name>A0A4W2E3E9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S23</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the 40S small ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3E9"/>
    <dbReference type="STRING" id="30522.A0A4W2E3E9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019838.1">
        <property type="protein sequence ID" value="ENSBIXP00000033433.1"/>
        <property type="gene ID" value="ENSBIXG00000016238.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="144" mass="16550" checksum="251FD26036FEA203" modified="2019-09-18" version="1">MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEEIGRYIGKRRFRKCIKWKKLLFNIMTFISLKTHHLFCYKSASLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3L2</accession>
    <name>A0A4W2E3L2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fatty acid desaturase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FADS6</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027374644.1">
        <property type="nucleotide sequence ID" value="XM_027518843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3L2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003291.1">
        <property type="protein sequence ID" value="ENSBIXP00000030696.1"/>
        <property type="gene ID" value="ENSBIXG00000012769.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055134.1">
        <property type="protein sequence ID" value="ENSBIXP00005027382.1"/>
        <property type="gene ID" value="ENSBIXG00005030725.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878033"/>
    <dbReference type="CTD" id="283985"/>
    <dbReference type="GeneTree" id="ENSGT00950000182990"/>
    <dbReference type="OMA" id="IHQMTHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF13">
        <property type="entry name" value="FATTY ACID DESATURASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fatty acid desaturase" evidence="5">
        <location>
            <begin position="64"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="342" mass="38614" checksum="84E6519E24D39ADF" modified="2019-09-18" version="1">MEPAGGAPRESARAEALLGELEARVQEVVRASSWWERHGVDCAILALSLLALPPGFLCLRSDSPLVFALGITILGVCHYTLTVKGSHLATHGALTESRGWSKVWTLFFVEVCTSFTAEYAKYGHVKMHHGYTNVLGLGDSSTWRLPCLNRYVYMFLAPLLIPIITPLVAVERLREVELRTALRTLGLISLGLYSQYWLLLNVSGFQSPSSALACMLITRSLLAHPYLHVNIFQHIGLPMFSPDKKPRRIHMMSLGVLNLPRLPVLDWAFGHSLISCHVEHHLFPRLSDNMCLKVKPVVSQFLHEKQLPYNEDSYLARFWLFLQRYEEFMVQTPPITELVGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5M5</accession>
    <name>A0A4W2E5M5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">GATOR2 complex protein WDR24</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">WD repeat-containing protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">WDR24</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalytic component of the GATOR2 complex, a multiprotein complex that acts as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex. In addition to its role in regulation of the mTORC1 complex, promotes the acidification of lysosomes and facilitates autophagic flux. Within the GATOR2 complex, WDR24 constitutes the catalytic subunit that mediates 'Lys-6'-linked ubiquitination of NPRL2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of the GATOR2 subcomplex, composed of MIOS, SEC13, SEH1L, WDR24 and WDR59. The GATOR2 complex interacts with CASTOR1 and CASTOR2; the interaction is negatively regulated by arginine. The GATOR2 complex interacts with SESN1, SESN2 and SESN3; the interaction is negatively regulated by amino acids. SESN1, SESN2 and SESN3 convey leucine availability via direct interaction with SEH1L and WDR24.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat WDR24 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027382888.1">
        <property type="nucleotide sequence ID" value="XM_027527087.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027382889.1">
        <property type="nucleotide sequence ID" value="XM_027527088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5M5"/>
    <dbReference type="SMR" id="A0A4W2E5M5"/>
    <dbReference type="STRING" id="30522.A0A4W2E5M5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014819.1">
        <property type="protein sequence ID" value="ENSBIXP00000027003.1"/>
        <property type="gene ID" value="ENSBIXG00000028984.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040395.1">
        <property type="protein sequence ID" value="ENSBIXP00005043732.1"/>
        <property type="gene ID" value="ENSBIXG00005006950.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883414"/>
    <dbReference type="CTD" id="84219"/>
    <dbReference type="GeneTree" id="ENSGT00940000159396"/>
    <dbReference type="OMA" id="EPMWLIS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061462">
        <property type="term" value="P:protein localization to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16693">
        <property type="entry name" value="mRING-H2-C3H3C2_WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000106">
        <property type="entry name" value="WD repeat domain 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 20" id="KW-0853">WD repeat</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="203"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="790" mass="88264" checksum="143BBCD52464F7A8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E681</accession>
    <name>A0A4W2E681_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">LOC113905021</name>
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    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_027562060.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSBIXT00000023156.1">
        <property type="protein sequence ID" value="ENSBIXP00000034862.1"/>
        <property type="gene ID" value="ENSBIXG00000017962.1"/>
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    <dbReference type="OMA" id="YELFVYV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Olfactory receptor"/>
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    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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    <dbReference type="PANTHER" id="PTHR26453">
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35068" checksum="357B87C6739E3932" modified="2019-09-18" version="1">MEHWNSTLGNGFILMGILNDSLSPELICATITALYTLALTSNCLLLLAITMDAQLHVPIYLLLGQLSLMDLLFTSVVTPKAFMDFLLSENTISFVGCALQMFLALTLGGAEDLLLAFMAYDRYVAICHPLNYMVLMRPRVCWLMVATSWILASLSAIVYTVYTMHYPFCKAREISHLLCEIPPLLKLACADTSRYELTVYVMGVTFLIPPLVAILASYTLILLTVLHMPSNEGRQKALVTCSSHLTVVGMFYGAATFMYVLPSPLHSPKEDNIISVFYTIVTPALNPLIYSLRNKEVMGALRRILSRYMLWTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6Q1</accession>
    <name>A0A4W2E6Q1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="13" type="primary">LOC113905197</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6Q1"/>
    <dbReference type="STRING" id="30522.A0A4W2E6Q1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045686.1">
        <property type="protein sequence ID" value="ENSBIXP00000034643.1"/>
        <property type="gene ID" value="ENSBIXG00000017691.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030523.1">
        <property type="protein sequence ID" value="ENSBIXP00005018247.1"/>
        <property type="gene ID" value="ENSBIXG00005021645.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286940"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="35"/>
            <end position="58"/>
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        <location>
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            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="173"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="51"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="329" mass="36203" checksum="479E49F2BDA29FAF" modified="2025-10-08" version="2">MPSTLGQTMVSPNHTFLDSSVFILMGIPGLEEFHLWLSLPVCLLGTATIVGNITILVVIVTEPALHKPMYLFLCMLSTIDLAASFSTVPKLLAILWCGAGHMSASACLAQMFFIHAFCMMESTVLLAMAFDRYVAICHPLRYTTVLTDTIIVRIGVVAMVRGSILMLPCPFLIQRLSFCQSHVIPHTYCEHMAVVKLACGDTRPNRVYGLTAALLVIGVDLFCIGLSYFLIARAVLRLSSHEARSKALGTCGSHVCVILISYTPALFSFFTHRFGHHVPLHTHIILANVYLLFPPALNPMVYGVKTKEIRERVVRVFQKGQGTGLKAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7A5</accession>
    <name>A0A4W2E7A5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1 and BTB domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Regulator of chromosome condensation and BTB domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RCBTB2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E7A5"/>
    <dbReference type="STRING" id="30522.A0A4W2E7A5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012945.1">
        <property type="protein sequence ID" value="ENSBIXP00000027576.1"/>
        <property type="gene ID" value="ENSBIXG00000000104.1"/>
    </dbReference>
    <dbReference type="OMA" id="SYNEHTA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd18529">
        <property type="entry name" value="BACK_RCBTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18354">
        <property type="entry name" value="BTB_POZ_RCBTB2_CHC1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000033">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000038">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000006">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000040">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF3">
        <property type="entry name" value="RCC1 AND BTB DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="121"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="174"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="227"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="279"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="397"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="554" mass="60540" checksum="16C00BEF61D3227E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9Q9</accession>
    <name>A0A4W2E9Q9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E9Q9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020312.1">
        <property type="protein sequence ID" value="ENSBIXP00000033570.1"/>
        <property type="gene ID" value="ENSBIXG00000016387.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="179"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="511" mass="58674" checksum="F8B36A5B6B93FFD1" modified="2019-09-18" version="1">MQKSKMAVWEALQIAVKRREAKSKGEKGRYKHLNAEFQRIARRDKKAFFGDQCKETEENNRMGKTRDFFKKIRDTKGTFHAKMSSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVMITDLEPDILECEVKWALKSITMNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQVWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQENIYFCFSDYAKAFDCVDHNKLWKILQEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAGYIMRNAGLEETQTGIKIAGRNINHFSYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPTTSWEIDGETVETVSDFILGGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EB67</accession>
    <name>A0A4W2EB67_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MAT2A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EB67"/>
    <dbReference type="SMR" id="A0A4W2EB67"/>
    <dbReference type="STRING" id="30522.A0A4W2EB67"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019298.1">
        <property type="protein sequence ID" value="ENSBIXP00000033199.1"/>
        <property type="gene ID" value="ENSBIXG00000015947.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005006239.1"/>
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    <sequence length="413" mass="45655" checksum="4A45969F06A60829" modified="2019-09-18" version="1">MNGQLNGFHDAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVRETIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGPMSFLDSWCSYRETSFLQRAVLLAGRRKSLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDX5</accession>
    <name>A0A4W2EDX5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSBIXG00000003171.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:olfactory receptor activity"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="49"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="225"/>
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            <end position="260"/>
        </location>
    </feature>
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        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035131.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="313" mass="35832" checksum="11E3985B1BBEE4C6" modified="2019-09-18" version="1">MEEHNRTVLSQFILMGITDRPELQAPLFVLFLIIYVISVVGNLGMVILTKVDSRLQTPMYFFLRHLALTDLGYSTAVGPKMLVNFVVDQNKISYYLCATQMGFFIMFIINELFILATMSYDRYVAICNPLLYTVIMSQRVCKVLVAIPYVYSTFVSLLITIKLFNSFFCGYNVISHFYCDNFPLLSLLCSNTHEIELMIMIFSAFNLTFTLAIVLVSYLLILAAILRMKSAEGRQKAFSTCGSHLTVVTVFYGTLTFMYVKPKSSHSFDTDKVASVFYTLVIPMLNPLIYSLRNKDVKYALQKMWRKLCSIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EF22</accession>
    <name>A0A4W2EF22_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF22"/>
    <dbReference type="SMR" id="A0A4W2EF22"/>
    <dbReference type="STRING" id="30522.A0A4W2EF22"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045666.1">
        <property type="protein sequence ID" value="ENSBIXP00000034623.1"/>
        <property type="gene ID" value="ENSBIXG00000017666.1"/>
    </dbReference>
    <dbReference type="OMA" id="KVNICAQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19761">
        <property type="entry name" value="Bbox2_TRIM5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16591">
        <property type="entry name" value="RING-HC_TRIM5-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000386">
        <property type="entry name" value="Tripartite motif-containing 5 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000144">
        <property type="entry name" value="Tripartite motif-containing 5 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="92"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="252"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="134"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="449" mass="52190" checksum="ACF27635C9F7365F" modified="2019-09-18" version="1">MASGIVVNLQQEVTCPICLELLTEPLSLDCGHSFCQACITANNMVSMNDQDEDRRCPVCRISYEPGNLRPNRHVANIVQRLREVKLSPEVEQERNLCLRHGEKLMLFCEEDREVICWLCARSQEHHGHHTFLMEEVAQDYQKKLQEALEKLREKQLEAEELEVKRQNVKAVFQKLRKNLKYEEVKELQKLKGKEEVGLRNLADSEHVLIQQSQLVRNLISDVEHHLKGSTMEMLQSIFSFPRSETLTLKKPRTLPKGQRRMCSALDLTDIQRVYNGEECYLSGCLWNSCDGKQLRYVCDLEPNNIYKNDDFEDHGVLSSPVITSQKYHWEADVSEKPAWILGAYGRKQHGYKMKFLFENKGNCQYVYWRYQPKYSYWIRGLKRICPLILTLSLSIPPHCIGVFLDYNTDVLFVNVTNQGILIYKLSSSSFTQKIFPYFNPGKCNAPITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGA1</accession>
    <name>A0A4W2EGA1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cilia and flagella associated protein 61</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CFAP61</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGA1"/>
    <dbReference type="STRING" id="30522.A0A4W2EGA1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046208.1">
        <property type="protein sequence ID" value="ENSBIXP00000035394.1"/>
        <property type="gene ID" value="ENSBIXG00000006057.1"/>
    </dbReference>
    <dbReference type="OMA" id="RWNEGQI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038884">
        <property type="entry name" value="CFAP61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056299">
        <property type="entry name" value="CFAP61_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032151">
        <property type="entry name" value="CFAP61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21178">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21178:SF8">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23150">
        <property type="entry name" value="CFAP61_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16092">
        <property type="entry name" value="CFAP61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 61 N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP61 dimerisation" evidence="3">
        <location>
            <begin position="1031"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1235" mass="141101" checksum="D33E311BD91A246B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EH85</accession>
    <name>A0A4W2EH85_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Solute carrier family 15 member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Intestinal H(+)/peptide cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Oligopeptide transporter, small intestine isoform</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Peptide transporter 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="47 48">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="47 48" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="47" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="47" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="28">Electrogenic proton-coupled amino-acid transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. Transports neutral and monovalently charged peptides with a proton to peptide stoichiometry of 1:1 or 2:1. Primarily responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen. Mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>L-alanyl-L-aspartate(out) + 2 H(+)(out) = L-alanyl-L-aspartate(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74363"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:71696"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>L-alanyl-L-leucyl-L-alanine(out) + H(+)(out) = L-alanyl-L-leucyl-L-alanine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71723"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:71724"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>L-alanyl-L-lysine(out) + H(+)(out) = L-alanyl-L-lysine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:72612"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-alanyl-L-proline(out) + H(+)(out) = L-alanyl-L-proline(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64420"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155848"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:64421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>L-alanyl-L-prolylglycine(out) + H(+)(out) = L-alanyl-L-prolylglycine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155849"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:64433"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-alanyl-L-valine(out) + H(+)(out) = L-alanyl-L-valine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72615"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:192471"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:72616"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>L-aspartyl-glycine(out) + 2 H(+)(out) = L-aspartyl-glycine(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71683"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191203"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:71684"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>L-leucyl-L-leucine(out) + H(+)(out) = L-leucyl-L-leucine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71715"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191208"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:71716"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>L-leucyl-L-proline(out) + H(+)(out) = L-leucyl-L-proline(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155847"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:64425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>L-lysyl-glycine(out) + H(+)(out) = L-lysyl-glycine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71679"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:71680"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="31">
            <dbReference type="Rhea" id="RHEA:71681"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>L-methionyl-L-phenylalanyl-L-methionine(out) + H(+)(out) = L-methionyl-L-phenylalanyl-L-methionine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71719"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191211"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:71720"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>L-phenylalanyl-L-leucine(out) + H(+)(out) = L-phenylalanyl-L-leucine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:190710"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:71700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>L-phenylalanyl-L-phenylalanine(out) + H(+)(out) = L-phenylalanyl-L-phenylalanine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191205"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:71708"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>L-tyrosylglycine(out) + H(+)(out) = L-tyrosylglycine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:71712"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>N(alpha)-formyl-L-methionyl-L-leucyl-L-phenylalanine(out) + 2 H(+)(out) = N(alpha)-formyl-L-methionyl-L-leucyl-L-phenylalanine(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:194314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>N-acetyl-D-muramoyl-L-alanyl-D-isoglutamine(out) + 2 H(+)(out) = N-acetyl-D-muramoyl-L-alanyl-D-isoglutamine(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155830"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a dipeptide(out) + H(+)(out) = a dipeptide(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64392"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:90799"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:64393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an L-amino acid tripeptide(out) + H(+)(out) = an L-amino acid tripeptide(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155837"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:64401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>carnosine(out) + H(+)(out) = carnosine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64404"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57485"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:64405"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>glycyl-L-aspartate(out) + 2 H(+)(out) = glycyl-L-aspartate(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71687"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:191204"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:71688"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71689"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>glycyl-L-glutamate(out) + 2 H(+)(out) = glycyl-L-glutamate(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71691"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73784"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71692"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>glycyl-L-glutamine(out) + H(+)(out) = glycyl-L-glutamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71671"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:71672"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:71673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>glycyl-L-leucine(out) + H(+)(out) = glycyl-L-leucine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:143163"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:71676"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>glycyl-L-proline(out) + H(+)(out) = glycyl-L-proline(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64428"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73779"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:64429"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glycyl-sarcosine(out) + H(+)(out) = glycyl-sarcosine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155838"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>glycylglycyl-L-isoleucine(out) + H(+)(out) = glycylglycyl-L-isoleucine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64436"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155850"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:64437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>glycylglycyl-L-proline(out) + H(+)(out) = glycylglycyl-L-proline(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64440"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155851"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:64441"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts (via extracellular domain region) with trypsin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="45">Membrane</location>
            <topology evidence="45">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 45">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EH85"/>
    <dbReference type="STRING" id="30522.A0A4W2EH85"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047372.1">
        <property type="protein sequence ID" value="ENSBIXP00000036967.1"/>
        <property type="gene ID" value="ENSBIXG00000020475.1"/>
    </dbReference>
    <dbReference type="OMA" id="FMTFDAD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000049">
        <property type="entry name" value="Solute carrier family 15 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000205">
        <property type="entry name" value="Solute carrier family 15 oligopeptide transporter member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004768">
        <property type="entry name" value="Oligopep_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00926">
        <property type="entry name" value="2A1704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 46" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="48" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 45 46" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 46" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 45" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="619"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <text evidence="10">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043140"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="92" mass="10275" checksum="7122AF87B0E24CF6" modified="2019-09-18" version="1">MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKTVAGGAWTYNTTSAVTVKSAIRRLKELKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKZ5</accession>
    <name>A0A4W2EKZ5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="14">Tetratricopeptide repeat protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TTC17</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in primary ciliogenesis by modulating actin polymerization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with CATIP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TTC17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKZ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025195.1">
        <property type="protein sequence ID" value="ENSBIXP00000035736.1"/>
        <property type="gene ID" value="ENSBIXG00000019059.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000053">
        <property type="entry name" value="Tetratricopeptide repeat domain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000061">
        <property type="entry name" value="Tetratricopeptide repeat domain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000111">
        <property type="entry name" value="tetratricopeptide repeat protein 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052630">
        <property type="entry name" value="TTC17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16091:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16091">
        <property type="entry name" value="TTC17 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="689"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="1085"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="848"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="848"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1141" mass="129398" checksum="09499B16B3C5B15C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMC4</accession>
    <name>A0A4W2EMC4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113878567</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD200R family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMC4"/>
    <dbReference type="SMR" id="A0A4W2EMC4"/>
    <dbReference type="STRING" id="30522.A0A4W2EMC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030394.1">
        <property type="protein sequence ID" value="ENSBIXP00000037979.1"/>
        <property type="gene ID" value="ENSBIXG00000021686.1"/>
    </dbReference>
    <dbReference type="OMA" id="MKAGTNM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150077">
        <property type="term" value="P:regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000584">
        <property type="entry name" value="Cell surface glycoprotein CD200 receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000769">
        <property type="entry name" value="Cell surface glycoprotein CD200 receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040012">
        <property type="entry name" value="CD200R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21462:SF2">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN CD200 RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21462">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN OX2 RECEPTOR PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021194439" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="27"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="139"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="355" mass="39220" checksum="AC9FD1D78B480F42" modified="2019-09-18" version="1">MPCTWITSDLQLRLILTLFFVAECLSAGMEGTKTSNNSMQQLDNGNHSSVSTTSSTERKQSTVTLYAEVKTSLSVLVDTKAVLTCPPVLWPSVLVVTWEIVLRDKPPCFGAYRRDTNQTTRGNCTDKRITWASRPDENPALQVDPVAITHDGNYTCQIVTSDGNFHHEYHLQVLVPPEVTLIQTEKGTAVCKAAAGKPAAQISWTPEGDCDTEQGPYWGDGTVTVQSTCRWGSRHVLNVSCSVSHLAGNKSLSIQLSQGAEIPAHLKNLYITAPIFIILIVVGSIWLLKISGCRKCKLKKTEHTPVVQEDEMEPYASYTEKNNPLYDITNRVKTSQVLQSEVDGMNLHTIYVPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ENM6</accession>
    <name>A0A4W2ENM6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease A-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC113899406</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the pancreatic ribonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408575.1">
        <property type="nucleotide sequence ID" value="XM_027552774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ENM6"/>
    <dbReference type="SMR" id="A0A4W2ENM6"/>
    <dbReference type="STRING" id="30522.A0A4W2ENM6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031206.1">
        <property type="protein sequence ID" value="ENSBIXP00000038340.1"/>
        <property type="gene ID" value="ENSBIXG00000022132.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899406"/>
    <dbReference type="OMA" id="PCAYRAR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06265">
        <property type="entry name" value="RNase_A_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.130.10:FF:000001">
        <property type="entry name" value="Ribonuclease pancreatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001427">
        <property type="entry name" value="RNaseA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023411">
        <property type="entry name" value="RNaseA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023412">
        <property type="entry name" value="RNaseA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437:SF24">
        <property type="entry name" value="RIBONUCLEASE PANCREATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00074">
        <property type="entry name" value="RnaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00794">
        <property type="entry name" value="RIBONUCLEASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00092">
        <property type="entry name" value="RNAse_Pc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54076">
        <property type="entry name" value="RNase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00127">
        <property type="entry name" value="RNASE_PANCREATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021452278" description="Ribonuclease A-domain domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease A-domain" evidence="9">
        <location>
            <begin position="27"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="150" mass="16377" checksum="F7A05C930FB83A83" modified="2019-09-18" version="1">MALKSLVVLPLLVLVLLLVRVQPSLGKESAAAKFERQHMDSGNSPSSSSNYCNLMMCCRKMTQGKCKPVNTFVHESLADVKAVCSQKKVTCKNGQTNCYQSKSTMRITDCRETGSSKYPNCAYKTTQVEKHIIVACGGKPSVPVHFDASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQT4</accession>
    <name>A0A4W2EQT4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large subunit GTPase 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LSG1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a GTPase. May act by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm during the 60S ribosomal subunit maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027393775.1">
        <property type="nucleotide sequence ID" value="XM_027537974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EQT4"/>
    <dbReference type="SMR" id="A0A4W2EQT4"/>
    <dbReference type="STRING" id="30522.A0A4W2EQT4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033300.1">
        <property type="protein sequence ID" value="ENSBIXP00000039246.1"/>
        <property type="gene ID" value="ENSBIXG00000023191.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040297.1">
        <property type="protein sequence ID" value="ENSBIXP00005043773.1"/>
        <property type="gene ID" value="ENSBIXG00005027405.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890197"/>
    <dbReference type="CTD" id="55341"/>
    <dbReference type="GeneTree" id="ENSGT00940000156442"/>
    <dbReference type="OMA" id="VNKADMM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="11">
        <location>
            <begin position="164"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="288"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="625"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="652" mass="74321" checksum="C908AC2B79A5E35A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV06</accession>
    <name>A0A4W2EV06_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP synthase F(1) complex subunit delta, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP synthase F1 subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-ATPase delta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATP5F1D</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Subunit delta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the central stalk subunit gamma, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Component of a complex composed at least by ATPIF1, ATP5F1A, ATP5F1B, ATP5F1C AND ATP5F1E.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402416.1">
        <property type="nucleotide sequence ID" value="XM_027546615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EV06"/>
    <dbReference type="SMR" id="A0A4W2EV06"/>
    <dbReference type="STRING" id="30522.A0A4W2EV06"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036848.1">
        <property type="protein sequence ID" value="ENSBIXP00000040789.1"/>
        <property type="gene ID" value="ENSBIXG00000024931.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044030.1">
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        <source>
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        <source>
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    <sequence length="168" mass="17612" checksum="7C47BBAD5A151608" modified="2019-09-18" version="1">MLPSALLRRPGLGRLVRQVRLYAEAAAAQAPAAGPGQMSFTFASPTQVFFNSANVRQVDVPTQTGAFGILAAHVPTLQVLRPGLVVVHAEDGTTSKYFVSSGSVTVNADSSVQLLAEEAVTLDMLDLGAAKANLEKAQSELLGAADEATRAEIQIRIEANEALVKALE</sequence>
</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="70"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="182" mass="20237" checksum="6EBD6DE5B849D603" modified="2019-09-18" version="1">MSSSSSIWDIIIDYLSLSSIWNYLQATLLGETSVPQQTNLGPLDNLAPAVQVILGISFLILLGVGMYALWKRSVQSIQKILLFAITLYKLYKKGSDFFQALLVNPEGSDLTLQDNNIFLSLGLQEKILKKLQTVENKVKDLEGMIISQKPTTKREYSSDHYCSCSDCQSPLPTSGFTSTSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZR9</accession>
    <name>A0A4W2EZR9_BOBOX</name>
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        <name evidence="15" type="primary">EPS15L1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Peripheral membrane protein</topology>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="UIM_dom"/>
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        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF69">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15-LIKE 1"/>
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    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="EFh"/>
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    <sequence length="939" mass="102515" checksum="EA5533E4AA13570A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="7">Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein. Within the PAT subcomplex, WDR83OS/Asterix binds to and redirects the substrate to a location behind the SEC61 complex.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Nucleolus</location>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="29"/>
            <end position="292"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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            <dbReference type="PROSITE" id="PS50833"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043872.1"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="469" mass="52789" checksum="E246AD51347D81EE" modified="2019-09-18" version="1">MGQSGRSRHQKRARAQAHLRNLEAYAAQPHSFVFARGRVGRSVQQLSMDLRRVMEPLTATRLQIRKKNSLKDCVAVAGPLGVTHFLIVSKTETNVYFKLMRLPGGPTLTFRINKYTLVRDVVSSLRRHRMHEQQFAHPPLLVLNSFGPHGMHVKLMATMFQNLFPSINVHKVNLNTIKRCLLINYNPDSQELDFRHYSIKVVPVGASRGMKKLLQEKFPNMSRLQDISELLATGAGLSESEAEPDGEHNITELPQAVAGRGNMRAQQSAVRLTEIGPRMTLELIKIQEGVGEGNVLFHRLVHKTEEELQAILAAKEEKLRLKAQRQDQQAQNVRRKREQREAHKKKSLAGMKRAQAEADGDSDAEDPGAPPEAERTSQQEEEEDEAEYFRQAVGEEPDEDMFPKAAKRRRPTGPPGKKQRLKERKPDRGSDRRPQKAKGKGPGAQAKVGPRGAARNRGQGRPRPLKRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F5E8</accession>
    <name>A0A4W2F5E8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nucleic acid binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NABP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SOSS-B family. SOSS-B1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398008.1">
        <property type="nucleotide sequence ID" value="XM_027542207.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398009.1">
        <property type="nucleotide sequence ID" value="XM_027542208.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398010.1">
        <property type="nucleotide sequence ID" value="XM_027542209.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398011.1">
        <property type="nucleotide sequence ID" value="XM_027542210.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398012.1">
        <property type="nucleotide sequence ID" value="XM_027542211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F5E8"/>
    <dbReference type="SMR" id="A0A4W2F5E8"/>
    <dbReference type="STRING" id="30522.A0A4W2F5E8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052854.1">
        <property type="protein sequence ID" value="ENSBIXP00000044263.1"/>
        <property type="gene ID" value="ENSBIXG00000028434.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018267.1">
        <property type="protein sequence ID" value="ENSBIXP00005010032.1"/>
        <property type="gene ID" value="ENSBIXG00005014783.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892826"/>
    <dbReference type="CTD" id="79035"/>
    <dbReference type="GeneTree" id="ENSGT00940000161079"/>
    <dbReference type="OMA" id="QSKAAQN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070200">
        <property type="term" value="P:establishment of protein localization to telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904355">
        <property type="term" value="P:positive regulation of telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000072">
        <property type="entry name" value="SOSS complex subunit B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF3">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="9">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="211" mass="22275" checksum="9721B80BBF7D8154" modified="2019-09-18" version="1">MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSAQQAPNKTVQNDSGPAAPQPPTGPPATSPASESQNGNGLSAPPGSGGGPHPPHTPSHPPSTRITRSQPNHTAAGPPGPSNNPVSNGKETRRSSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F7L9</accession>
    <name>A0A4W2F7L9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein kinase PDIK1L</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PDLIM1-interacting kinase 1-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PDIK1L</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027375669.1">
        <property type="nucleotide sequence ID" value="XM_027519868.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027375670.1">
        <property type="nucleotide sequence ID" value="XM_027519869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F7L9"/>
    <dbReference type="SMR" id="A0A4W2F7L9"/>
    <dbReference type="STRING" id="30522.A0A4W2F7L9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029745.1">
        <property type="protein sequence ID" value="ENSBIXP00000037703.1"/>
        <property type="gene ID" value="ENSBIXG00000021328.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014489.1">
        <property type="protein sequence ID" value="ENSBIXP00005001301.1"/>
        <property type="gene ID" value="ENSBIXG00005029304.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878632"/>
    <dbReference type="CTD" id="149420"/>
    <dbReference type="GeneTree" id="ENSGT00940000158412"/>
    <dbReference type="OMA" id="MVQKMAH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13977">
        <property type="entry name" value="STKc_PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000174">
        <property type="entry name" value="Serine/threonine-protein kinase PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000165">
        <property type="entry name" value="Serine/threonine-protein kinase PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF58">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="8"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="341" mass="38559" checksum="CBAE710DDB8CCAE8" modified="2019-09-18" version="1">MVSSQPKYDLIREVGRGSYGVVYEAVIRKTSARVAVKKIRCHAPENVELALREFWALSSIKSQHPNVIHLEECILQKDGMVQKMSHGSNSSLYLQLVETSLKGEIAFDPRSAYYLWFVMDFCDGGDMNEYLLSRKPNRKTNTSFMLQLSSALAFLHKNQIIHRDLKPDNILISQSRLDTSDLEPTLKVADFGLSKVCSASGQNPEEPVSVNKCFLSTACGTDFYMAPEVWEGHYTAKADIFALGIIIWAMLERITFIDTETKKELLGSYVKQGTEIVPVGEALLENPKMELLIPVKKKSMNGRMKQLIKEMLAANPQDRPDAFELELRLVQIAFKDNSWET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FAR9</accession>
    <name>A0A4W2FAR9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein JTB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">JTB</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1. Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with AURKA, AURKB, BIRC5 and INCENP. May be a component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the JTB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388533.1">
        <property type="nucleotide sequence ID" value="XM_027532732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FAR9"/>
    <dbReference type="SMR" id="A0A4W2FAR9"/>
    <dbReference type="STRING" id="30522.A0A4W2FAR9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039419.1">
        <property type="protein sequence ID" value="ENSBIXP00000023704.1"/>
        <property type="gene ID" value="ENSBIXG00000026104.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012213.1">
        <property type="protein sequence ID" value="ENSBIXP00005002455.1"/>
        <property type="gene ID" value="ENSBIXG00005000281.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886498"/>
    <dbReference type="CTD" id="10899"/>
    <dbReference type="GeneTree" id="ENSGT00390000016136"/>
    <dbReference type="OMA" id="EECHPCS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.220:FF:000001">
        <property type="entry name" value="Jumping translocation breakpoint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008657">
        <property type="entry name" value="JTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13041">
        <property type="entry name" value="JTB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13041:SF3">
        <property type="entry name" value="PROTEIN JTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05439">
        <property type="entry name" value="JTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611305" description="Protein JTB" evidence="20">
        <location>
            <begin position="31"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="146" mass="16339" checksum="C1AD2DDF9057015B" modified="2019-09-18" version="1">MPAGAGRRGLPQGHHLCWLLCAFTLRLCQAEAPVREEKLSVSTSNLPCWLVEEFVVAEECAPCSNFQAKTTPECGSTGYVEKITCSSSKRSEFKSCRSALMEQRLFWKFEGAVIGLALVFACLVIVRQRQLDRKALEKVRKQIESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G8E5</accession>
    <name>A0A4W2G8E5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MPC1L</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027390066.1">
        <property type="nucleotide sequence ID" value="XM_027534265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G8E5"/>
    <dbReference type="STRING" id="30522.A0A4W2G8E5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009854.1">
        <property type="protein sequence ID" value="ENSBIXP00000003432.1"/>
        <property type="gene ID" value="ENSBIXG00000009852.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022869.1">
        <property type="protein sequence ID" value="ENSBIXP00005013155.1"/>
        <property type="gene ID" value="ENSBIXG00005017292.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887235"/>
    <dbReference type="CTD" id="347411"/>
    <dbReference type="GeneTree" id="ENSGT00940000163590"/>
    <dbReference type="OMA" id="LMACHCT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="181" mass="19181" checksum="4BC407D3E38EFCBA" modified="2019-09-18" version="1">MAAVVALCRKAMETVKTKEFRDYLASTHFWGPVANWGLPLAAFRDMRASPDIISGRMTTALIFYSMAFMRFAYRVQPRNLLLMACHGTNIVAQSMQAGRYLNYHYGGGTTAATTAAVSAASATSTGSVDSSATSTGSVDSSATSTGSVDSTAATTPAAEDPVAHSNCQEITCCYLVTWDCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GCC0</accession>
    <name>A0A4W2GCC0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cathelicidin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GCC0"/>
    <dbReference type="STRING" id="30522.A0A4W2GCC0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009560.1">
        <property type="protein sequence ID" value="ENSBIXP00000028677.1"/>
        <property type="gene ID" value="ENSBIXG00000010002.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048146.1">
        <property type="protein sequence ID" value="ENSBIXP00005016307.1"/>
        <property type="gene ID" value="ENSBIXG00005020021.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000410"/>
    <dbReference type="OMA" id="WAWTLER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000003">
        <property type="entry name" value="Cathelicidin antimicrobial peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001894">
        <property type="entry name" value="Cathelicidin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018216">
        <property type="entry name" value="Cathelicidin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10206">
        <property type="entry name" value="CATHELICIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10206:SF2">
        <property type="entry name" value="CATHELICIDIN ANTIMICROBIAL PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00666">
        <property type="entry name" value="Cathelicidins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00946">
        <property type="entry name" value="CATHELICIDINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00947">
        <property type="entry name" value="CATHELICIDINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611611" evidence="8">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="146" mass="16510" checksum="64C91E3708013E76" modified="2019-09-18" version="1">METQRASLSLGRWSLWLLLLGLALPSASAQALSYREAVLRAVDQLNERSSEANLYRLLDPPTLILDQVEDPGARKPVSFRVKETVCPRPSLQPPEQCDFKENGVSLGPRHLDFHKPLFRPALLGWAWTLERPRLPVGLSFLICLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GEQ9</accession>
    <name>A0A4W2GEQ9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Coiled-coil domain containing 126</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCDC126</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027395712.1">
        <property type="nucleotide sequence ID" value="XM_027539911.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395713.1">
        <property type="nucleotide sequence ID" value="XM_027539912.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395714.1">
        <property type="nucleotide sequence ID" value="XM_027539913.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395715.1">
        <property type="nucleotide sequence ID" value="XM_027539914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GEQ9"/>
    <dbReference type="SMR" id="A0A4W2GEQ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039531.1">
        <property type="protein sequence ID" value="ENSBIXP00000042019.1"/>
        <property type="gene ID" value="ENSBIXG00000026166.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028904.1">
        <property type="protein sequence ID" value="ENSBIXP00005017197.1"/>
        <property type="gene ID" value="ENSBIXG00005020716.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891616"/>
    <dbReference type="CTD" id="90693"/>
    <dbReference type="GeneTree" id="ENSGT00940000153470"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042759">
        <property type="entry name" value="CCDC126"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027833">
        <property type="entry name" value="MGT5A-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46941">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46941:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15027">
        <property type="entry name" value="MGT5A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="MGT5A-like N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15593" checksum="4CF8F6FA426C3065" modified="2019-09-18" version="1">MVFTISRKSMSQKLSLLLLVFGLLWGLMLLHYIFQRPRHQSSVKLREQILDLSKKYVKALAEENKNTMDVENGASMAGYADLRRTIAVLLDDILQRLVKLENKVDYIVVNGSAASTTSGTSGNPVPLTTNKRVKASGSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GQ41</accession>
    <name>A0A4W2GQ41_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Semaphorin-3C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SEMA3C</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with PLXND1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395611.1">
        <property type="nucleotide sequence ID" value="XM_027539810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GQ41"/>
    <dbReference type="SMR" id="A0A4W2GQ41"/>
    <dbReference type="STRING" id="30522.A0A4W2GQ41"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042099.1">
        <property type="protein sequence ID" value="ENSBIXP00000043408.1"/>
        <property type="gene ID" value="ENSBIXG00000027520.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033634.1">
        <property type="protein sequence ID" value="ENSBIXP00005020322.1"/>
        <property type="gene ID" value="ENSBIXG00005023456.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891587"/>
    <dbReference type="CTD" id="10512"/>
    <dbReference type="GeneTree" id="ENSGT00940000159379"/>
    <dbReference type="OMA" id="AMYIDFM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <sequence length="751" mass="85237" checksum="C2EBBE73F3179397" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GUE4</accession>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <text evidence="13">Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections. Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS. Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines. Involved in memory-related synaptic plasticity in the hippocampus.</text>
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            <location evidence="4">Growth cone</location>
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        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000032">
        <property type="entry name" value="Drebrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF1">
        <property type="entry name" value="DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="17">
        <location>
            <begin position="70"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="274"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="605"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="642"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="726" mass="79420" checksum="8998CF29A8084D8B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GYY2</accession>
    <name>A0A4W2GYY2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-defensin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DEFB127</name>
        <name evidence="12" type="synonym">LOC113902703</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Has antibacterial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the beta-defensin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GYY2"/>
    <dbReference type="SMR" id="A0A4W2GYY2"/>
    <dbReference type="STRING" id="30522.A0A4W2GYY2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000037387.1"/>
        <property type="gene ID" value="ENSBIXG00000020938.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037269.1">
        <property type="protein sequence ID" value="ENSBIXP00005022771.1"/>
        <property type="gene ID" value="ENSBIXG00005004359.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064502"/>
    <dbReference type="OMA" id="KKCWGEY"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050544">
        <property type="entry name" value="Beta-defensin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025933">
        <property type="entry name" value="Beta_defensin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001:SF3">
        <property type="entry name" value="BETA-DEFENSIN 123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001">
        <property type="entry name" value="BETA-DEFENSIN 123-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13841">
        <property type="entry name" value="Defensin_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="5 10" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="7 10" id="KW-0211">Defensin</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516542" description="Beta-defensin" evidence="10">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-defensin" evidence="11">
        <location>
            <begin position="23"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU231113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="99" mass="11856" checksum="DB40426EE9BC270D" modified="2019-09-18" version="1">MRLLLIIAILLFQKFTVTEQLKRCWNQHIHGYCRKICRTTEVREVLCENGRYCCISIVELEERKKITRPPRPKLRTLALTFSQDYLIEQNSTHSHRKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H0C1</accession>
    <name>A0A4W2H0C1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">MAK</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027380251.1">
        <property type="nucleotide sequence ID" value="XM_027524450.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380252.1">
        <property type="nucleotide sequence ID" value="XM_027524451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H0C1"/>
    <dbReference type="SMR" id="A0A4W2H0C1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013485.1">
        <property type="protein sequence ID" value="ENSBIXP00000001666.1"/>
        <property type="gene ID" value="ENSBIXG00000008227.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040687.1">
        <property type="protein sequence ID" value="ENSBIXP00005025129.1"/>
        <property type="gene ID" value="ENSBIXG00005027680.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881408"/>
    <dbReference type="CTD" id="4117"/>
    <dbReference type="GeneTree" id="ENSGT00940000156581"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="309"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="412"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025129.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="623" mass="69774" checksum="1768C08B77C5A083" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H8J5</accession>
    <name>A0A4W2H8J5_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat domain 23 like</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">TTC23L</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="359" mass="40425" checksum="5DA2AA4D3C506F3A" modified="2019-09-18" version="1">MRASPIRIPTVTSDTDWDFCFHLSQQTKIPEHQRTDESSPTSGSEESEEDTKAKEKVIDHMSHPREKLAQSQKKIAQLIKGKKNIEANKELIRCVTLSRIIFGEEHWKCAQALATLAYGYLTLRGLPAQAKKHAESAKNVLLTWKGTTTSDKEEKQILETLVMLYYTLGVVCLLQNHGREAYFNLQKSERNMKELRESYKGGTCGLQVSEKDLTVALGRASLANCRLNLALVYFEKAVDNVIANKGDSTSELVSLYQEIAQIEQLRRNHEQAIQYLHQAHSICVSLYTEVSPQAAEASALLAKAYAMSGEVQHKDAVGLYFMKSITAYQTTLGPEDYETLTTVEEFCKWLVQNGEKRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H8L0</accession>
    <name>A0A4W2H8L0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2H8L0"/>
    <dbReference type="STRING" id="30522.A0A4W2H8L0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024558.1">
        <property type="protein sequence ID" value="ENSBIXP00000013891.1"/>
        <property type="gene ID" value="ENSBIXG00000018736.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043889.1">
        <property type="protein sequence ID" value="ENSBIXP00005027158.1"/>
        <property type="gene ID" value="ENSBIXG00005005230.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153544"/>
    <dbReference type="OMA" id="ILHKAHM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF221">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="11"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="160" mass="18333" checksum="86850AC581A13450" modified="2019-09-18" version="1">MRQKKLGLEDFESLKVIGRGAFGEVWLVQKKDTGHVYAMKILHKAHMLEKEQVGHNRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLIKKDRLREEETQFYVAETVLALDSIHQLGFIHRDLMTGRTKTTVSTMLGTKGCPASLEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I3G2</accession>
    <name>A0A4W2I3G2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MORN3</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with MEIG1. Interacts with TP53, MDM2 and SIRT1; the interactions mediate post-transcriptional modifications of TP53 by MDM2 and SIRT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2I3G2"/>
    <dbReference type="SMR" id="A0A4W2I3G2"/>
    <dbReference type="STRING" id="30522.A0A4W2I3G2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044192.1">
        <property type="protein sequence ID" value="ENSBIXP00000009514.1"/>
        <property type="gene ID" value="ENSBIXG00000015175.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028535.1">
        <property type="protein sequence ID" value="ENSBIXP00005038430.1"/>
        <property type="gene ID" value="ENSBIXG00005007726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159285"/>
    <dbReference type="OMA" id="GHGRFFH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901797">
        <property type="term" value="P:negative regulation of signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072331">
        <property type="term" value="P:signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000026">
        <property type="entry name" value="MORN repeat containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="241" mass="27829" checksum="DD04B69CDBAFAFC2" modified="2019-09-18" version="1">MPIFKCPQKSEPLWKEWDQKAQKNGLRHQVFAVNGDHYVGEWKDNVKHGKGTQVWKKNGAIYEGDWKSGKRDGYGTLSLPDQETGKYKRAYSGWWKGDKKCGYGIQFFGPKEYYEGDWCGNQRSGWGRMYYSNGDIYEGQWRNDKPEGEGMLRLKNGNRYEGNWQRGVKNGSGRFFHLDHGQLFEGFWVDDVAKCGTMIDFGRDEAPQPTQFPIPEVKILDPDGVLEEALAMFKKTKEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IEX7</accession>
    <name>A0A4W2IEX7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7</fullName>
            <ecNumber evidence="13">1.14.11.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">JmjC domain-containing protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Jumonji domain-containing protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">JMJD7</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as a Fe(2+) and 2-oxoglutarate-dependent monooxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer; disulfide-linked. Interacts with DRG1 and DRG2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.63" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027409418.1">
        <property type="nucleotide sequence ID" value="XM_027553617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IEX7"/>
    <dbReference type="SMR" id="A0A4W2IEX7"/>
    <dbReference type="STRING" id="30522.A0A4W2IEX7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029562.1">
        <property type="protein sequence ID" value="ENSBIXP00000017197.1"/>
        <property type="gene ID" value="ENSBIXG00000021213.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052856.1">
        <property type="protein sequence ID" value="ENSBIXP00005043875.1"/>
        <property type="gene ID" value="ENSBIXG00005027641.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900136"/>
    <dbReference type="CTD" id="100137047"/>
    <dbReference type="GeneTree" id="ENSGT00900000141196"/>
    <dbReference type="OMA" id="YWHDMEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106155">
        <property type="term" value="F:peptidyl-lysine 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000059">
        <property type="entry name" value="jmjC domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF99">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND (3S)-LYSYL HYDROXYLASE JMJD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="17">
        <location>
            <begin position="128"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="316" mass="36043" checksum="65E2C4F4EF7A3321" modified="2019-09-18" version="1">MAEAALDAVRRELREFPVAARELSVPLAVPYLDEPPSPLHFYRDWVCPNRPCIIRNALQHWPALRKWSLPYLRATVGSTEVSVAVTPDGYADAVRGDRFVMPAERRLPLSHVLDVLEGRAQHPGVLYVQKQCSNLPTELPQLLPDVEPHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLLHPPSDRPFIPYELYTQATYQLTEEGSFRMVDEEAMEKVPWIPLDPLAPDLARYPSYCQAQALRCTVRAGEMLYLPALWFHHVQQSHGCIAVNFWYDMEYDLKYSYFQLLDSLTKVSGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IFF2</accession>
    <name>A0A4W2IFF2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TCEA1</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with EAF2. Associates with UBR5 and forms a transcription regulatory complex made of CDK9, Pol II, UBR5 and TCEA1/TFIIS. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416788.1">
        <property type="nucleotide sequence ID" value="XM_027560987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IFF2"/>
    <dbReference type="SMR" id="A0A4W2IFF2"/>
    <dbReference type="STRING" id="30522.A0A4W2IFF2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022085.1">
        <property type="protein sequence ID" value="ENSBIXP00000034408.1"/>
        <property type="gene ID" value="ENSBIXG00000006134.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034204.1">
        <property type="protein sequence ID" value="ENSBIXP00005040950.1"/>
        <property type="gene ID" value="ENSBIXG00005023901.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904171"/>
    <dbReference type="CTD" id="6917"/>
    <dbReference type="GeneTree" id="ENSGT00940000155121"/>
    <dbReference type="OMA" id="RFVVMTH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.30:FF:000001">
        <property type="entry name" value="Transcription elongation factor A (SII), 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000002">
        <property type="entry name" value="Transcription elongation factor A (SII), 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006289">
        <property type="entry name" value="TFSII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01385">
        <property type="entry name" value="TFSII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR A PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="21">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="22">
        <location>
            <begin position="140"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="20">
        <location>
            <begin position="259"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="76"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="301" mass="33891" checksum="87C98D336FD4CDCF" modified="2019-09-18" version="1">MEDEVIRIAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTDKDSEEKKKDTAVTSQNSPEAREESSSSGNMSSRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IM21</accession>
    <name>A0A4W2IM21_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Dynamin-like GTPase OPA1, mitochondrial</fullName>
            <ecNumber evidence="3">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">OPA1</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Oligomeric complex consisting of membrane-bound and soluble forms of OPA1. Interacts with RCC1L; RCC1L acts as a guanine nucleotide exchange factor (GEF) for OPA1 by exchanging bound GDP for free GTP. Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms. Interacts with PRELID1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027394002.1">
        <property type="nucleotide sequence ID" value="XM_027538201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IM21"/>
    <dbReference type="SMR" id="A0A4W2IM21"/>
    <dbReference type="STRING" id="30522.A0A4W2IM21"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033544.1">
        <property type="protein sequence ID" value="ENSBIXP00000039313.1"/>
        <property type="gene ID" value="ENSBIXG00000023290.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039355.1">
        <property type="protein sequence ID" value="ENSBIXP00005043190.1"/>
        <property type="gene ID" value="ENSBIXG00005026898.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890276"/>
    <dbReference type="CTD" id="4976"/>
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        <source>
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        <source>
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        <source>
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    <sequence length="1015" mass="117520" checksum="FA346EBF8C73A310" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BP39</accession>
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        <recommendedName>
            <fullName evidence="12">Tubulin tyrosine ligase like 3</fullName>
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    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="8">
            <text>L-glutamyl-[protein] + glycine + ATP = glycyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:167890"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67181"/>
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    </comment>
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        <subcellularLocation>
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            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
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        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
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        <property type="entry name" value="TTL"/>
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    <keyword evidence="3" id="KW-0436">Ligase</keyword>
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        <location>
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        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="62947" checksum="46CCCC2F4671CA08" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQD9</accession>
    <name>A0A4W2BQD9_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4 14">Protein-tyrosine sulfotransferase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">TPST1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 14">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_027382768.1">
        <property type="nucleotide sequence ID" value="XM_027526967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQD9"/>
    <dbReference type="SMR" id="A0A4W2BQD9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014258.1">
        <property type="protein sequence ID" value="ENSBIXP00000001331.1"/>
        <property type="gene ID" value="ENSBIXG00000028674.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042328.1">
        <property type="protein sequence ID" value="ENSBIXP00005044733.1"/>
        <property type="gene ID" value="ENSBIXG00005028601.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883325"/>
    <dbReference type="CTD" id="8460"/>
    <dbReference type="GeneTree" id="ENSGT00390000006030"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000290">
        <property type="entry name" value="Protein-tyrosine sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF4">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="370" mass="42179" checksum="012634A621DDBAA0" modified="2019-09-18" version="1">MVGKLKQNLLLACLVISSVTVFYLGQHAMECHHRIEERSQPLKLENIKTTVRTALDIKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQIWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLARLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTVLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKILENTRRVYKGEFQLPEFLKEKPQSEQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRB2</accession>
    <name>A0A4W2BRB2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine. L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator. May also act on O-phospho-D-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-D-serine + H2O = D-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58680"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24874"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-serine + H2O = L-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21209"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRB2"/>
    <dbReference type="STRING" id="30522.A0A4W2BRB2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014088.1">
        <property type="protein sequence ID" value="ENSBIXP00000001410.1"/>
        <property type="gene ID" value="ENSBIXG00000028592.1"/>
    </dbReference>
    <dbReference type="OMA" id="TFCEDES"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04309">
        <property type="entry name" value="HAD_PSP_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.210:FF:000002">
        <property type="entry name" value="Phosphoserine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000077">
        <property type="entry name" value="Phosphoserine phosphatase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000114">
        <property type="entry name" value="Phosphoserine phosphatase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.210">
        <property type="entry name" value="Phosphoserine phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="287" mass="31834" checksum="BB02DD9E5C1F2C86" modified="2019-09-18" version="1">MVSHSELRNLFCSADAVCFDVDSTVIQEEGIDELAKFCGVEDAVSEMTRQAMGGAVPFKAALTQRLALIQPSREQVQRLLAEHPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPSTNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIVMVGDGATDMEACPPADAFIGFGGNVIRQQVKDNAECIQYFRYFKYSSRNNLQARNPGVEILLSTEVVTAHPPSAPPLGAVLSVRDGSPFLPSWYLSHRKCYKDFFKKGNMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BS21</accession>
    <name>A0A4W2BS21_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BS21"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011084.1">
        <property type="protein sequence ID" value="ENSBIXP00000002849.1"/>
        <property type="gene ID" value="ENSBIXG00000000274.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="369" mass="42571" checksum="EA251A7592E0B909" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKAFECVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEETQAGIKIARRNINNLRYADDTTLMAESEKELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGETGETVSDFIFLDSKITADGDCSHEIKRHLLLGRKVMINLDSIFKSKDITLPTKVHLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRADSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVMDREAWRAAIHGSQRVGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUT9</accession>
    <name>A0A4W2BUT9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="23">NudE neurodevelopment protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">NDE1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383001.1">
        <property type="nucleotide sequence ID" value="XM_027527200.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383002.1">
        <property type="nucleotide sequence ID" value="XM_027527201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUT9"/>
    <dbReference type="SMR" id="A0A4W2BUT9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008873.1">
        <property type="protein sequence ID" value="ENSBIXP00000003931.1"/>
        <property type="gene ID" value="ENSBIXG00000007219.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017200.1">
        <property type="protein sequence ID" value="ENSBIXP00005009299.1"/>
        <property type="gene ID" value="ENSBIXG00005014248.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883466"/>
    <dbReference type="CTD" id="54820"/>
    <dbReference type="GeneTree" id="ENSGT00390000000111"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007100">
        <property type="term" value="P:mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF2">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="19" id="KW-0137">Centromere</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="22">
        <location>
            <begin position="134"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="32"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="181"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="279"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="346" mass="38687" checksum="351CD5966C8D00D8" modified="2019-09-18" version="1">MADSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEESREYEAELETQLQQTESKNRDLLMENNHLRMELETIKEKFKTQHSEGYRQISALEDDLAQTKAIKDKLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVDAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGTFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNFVYDQSPGRASGPASGRGSKNRDSVDRRPGGSNVPLGDKGLGKRLEFGKPSSNVSSPSLPSAQGVVKMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVU9</accession>
    <name>A0A4W2BVU9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Group XIIB secretory phospholipase A2-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Group XIII secretory phospholipase A2-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">sPLA2-GXIIB</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Not known; does not seem to have catalytic activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVU9"/>
    <dbReference type="SMR" id="A0A4W2BVU9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007894.1">
        <property type="protein sequence ID" value="ENSBIXP00000004402.1"/>
        <property type="gene ID" value="ENSBIXG00000010750.1"/>
    </dbReference>
    <dbReference type="OMA" id="ICQYRCR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000003">
        <property type="entry name" value="Group XIIB secretory phospholipase A2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010711">
        <property type="entry name" value="PLA2G12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824">
        <property type="entry name" value="GROUP XII SECRETORY PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824:SF2">
        <property type="entry name" value="GROUP XIIB SECRETORY PHOSPHOLIPASE A2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06951">
        <property type="entry name" value="PLA2G12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021365278" description="Group XIIB secretory phospholipase A2-like protein" evidence="12">
        <location>
            <begin position="20"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="195" mass="21906" checksum="6372B9EDCFECDB6E" modified="2019-09-18" version="1">MTWAISFLILWLSLAGGLAQSDTSPDEEESYSDWGLRHIRGSFESVNSYFDSFMELLGGKNGVCQYRCRYGKAPMPRPGYKPQEPNGCSSYFLGLKVPERMDLGIPAMTKCCNQLDVCYDTCGANKYRCDAKFRWCLHSICSDLKRSLGFVSNVEVACDSLADTVFNTVWTLGCRPFMNSQRAACICAEEEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWH5</accession>
    <name>A0A4W2BWH5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tumor necrosis factor receptor superfamily member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">B-cell surface antigen CD40</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">CD40L receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer and homodimer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts with TRAF6 and MAP3K8; the interaction is required for ERK activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BWH5"/>
    <dbReference type="SMR" id="A0A4W2BWH5"/>
    <dbReference type="STRING" id="30522.A0A4W2BWH5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007423.1">
        <property type="protein sequence ID" value="ENSBIXP00000004626.1"/>
        <property type="gene ID" value="ENSBIXG00000007098.1"/>
    </dbReference>
    <dbReference type="OMA" id="CQRNTIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005031">
        <property type="term" value="F:tumor necrosis factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023035">
        <property type="term" value="P:CD40 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048535">
        <property type="term" value="P:lymph node development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060749">
        <property type="term" value="P:mammary gland alveolus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072674">
        <property type="term" value="P:multinuclear osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045780">
        <property type="term" value="P:positive regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070555">
        <property type="term" value="P:response to interleukin-1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13411">
        <property type="entry name" value="TNFRSF11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000041">
        <property type="entry name" value="Tumor necrosis factor receptor superfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041648">
        <property type="entry name" value="RANK_CRD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022361">
        <property type="entry name" value="TNFR_11A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053075">
        <property type="entry name" value="TNFRSF11A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034040">
        <property type="entry name" value="TNFRSF11A_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47134">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47134:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18278">
        <property type="entry name" value="RANK_CRD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01974">
        <property type="entry name" value="TNFACTORR11A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="19">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="16">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="305"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="423"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="512"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="479"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="588" mass="61857" checksum="097692953AF94EB2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYL8</accession>
    <name>A0A4W2BYL8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Synaptopodin 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SYNPO2L</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Actin-associated protein that may play a role in modulating actin-based shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386362.1">
        <property type="nucleotide sequence ID" value="XM_027530561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYL8"/>
    <dbReference type="STRING" id="30522.A0A4W2BYL8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008164.1">
        <property type="protein sequence ID" value="ENSBIXP00000004276.1"/>
        <property type="gene ID" value="ENSBIXG00000010639.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019087.1">
        <property type="protein sequence ID" value="ENSBIXP00005010608.1"/>
        <property type="gene ID" value="ENSBIXG00005015210.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885315"/>
    <dbReference type="CTD" id="79933"/>
    <dbReference type="GeneTree" id="ENSGT00950000183054"/>
    <dbReference type="OMA" id="MVQNMDG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10820">
        <property type="entry name" value="PDZ_SYNPO2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000137">
        <property type="entry name" value="Synaptopodin 2-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217:SF10">
        <property type="entry name" value="SYNAPTOPODIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="313"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="919"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="385"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="772"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="973" mass="102161" checksum="236EBBCFF2F39E7B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C0H1</accession>
    <name>A0A4W2C0H1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome biogenesis protein BOP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Block of proliferation 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">BOP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8">Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. The complex is held together by BOP1, which interacts with PES1 via its N-terminal domain and with WDR12 via a high-affinity interaction between the seven-bladed beta-propeller domains of the 2 proteins. The NOP7 complex associates with the 66S pre-ribosome. The PeBoW complex associates with DDX27, BOP1 interacts directly with DDX27.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C0H1"/>
    <dbReference type="SMR" id="A0A4W2C0H1"/>
    <dbReference type="STRING" id="30522.A0A4W2C0H1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004198.1">
        <property type="protein sequence ID" value="ENSBIXP00000006208.1"/>
        <property type="gene ID" value="ENSBIXG00000012339.1"/>
    </dbReference>
    <dbReference type="OMA" id="MRPAKGE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="11">
        <location>
            <begin position="151"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="416"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="666"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="49"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="Ensembl" id="ENSBIXP00000006951.1"/>
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    <sequence length="365" mass="42551" checksum="4638B325F295BBDB" modified="2019-09-18" version="1">MLLVKLTIDRAMAEAEDTKSKQRGALGAAEVTSDAQRRQNNGGLRKEQLAVQTQPSRLPLVTDSEWLALSAEEQLAWAKNIQDPRIAVGSHSPLEKKIKNLGGVHSPEVRKLLAQKTQQERERLDQLKAMSFDFRFAQAEAYYYQRYQEMLEEACKHKMVLEGEIKTEEKERRPQNEKKEDAKKWHYLVSERELNQIQKHIHRAERARGLRDHNYQLLPQSISSEIPSHEVLNLKKDVSTENIQKTHKAKAKQYRVAWAKKQIKGHQDRMIRGRELTEQRNGQRRAQKIPAQAPAFLKPRVKKKEVKEYEWVTTYPIVQPYAEPLLEVTVLMEKSKKENKIGKPLRRELLSIPSFLRSQLQKHKV</sequence>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060429"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074262"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50156"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008024.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
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        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Ruminantia</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C8G6"/>
    <dbReference type="STRING" id="30522.A0A4W2C8G6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011616.1">
        <property type="protein sequence ID" value="ENSBIXP00000002588.1"/>
        <property type="gene ID" value="ENSBIXG00000009063.1"/>
    </dbReference>
    <dbReference type="OMA" id="GYELGSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052313">
        <property type="entry name" value="GPIb-IX-V_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22650">
        <property type="entry name" value="GLYCOPROTEIN IB BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22650:SF6">
        <property type="entry name" value="PLATELET GLYCOPROTEIN IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021451067" evidence="13">
        <location>
            <begin position="17"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="14">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="15">
        <location>
            <begin position="85"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="175" mass="18725" checksum="39E2D1E8977BFB47" modified="2019-09-18" version="1">MPAWVALLLFWAAAEASEDCPAACACRALDTMGLQVDCRGRGLEVLPALPAPTRELLLTNNSLQSVPPGAFDHLPQLQALDLAHNPWRCDCGLVYLRLWLEDRAPEQLLRLRCAGPAHAADRTPAQLSGSELGGCGWTLRESWAGPGSWWDAALVVVAALGLALLVSLLCTIPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CB92</accession>
    <name>A0A4W2CB92_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Endosialin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Tumor endothelial marker 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Interacts with PDGFRA; this interaction promotes PDGF receptor signaling pathway. Interacts with integrin beta-1/ITGB1. Interacts with insulin receptor/INSR; this interaction diminishes INSR autophosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CB92"/>
    <dbReference type="STRING" id="30522.A0A4W2CB92"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009561.1">
        <property type="protein sequence ID" value="ENSBIXP00000003579.1"/>
        <property type="gene ID" value="ENSBIXG00000010001.1"/>
    </dbReference>
    <dbReference type="OMA" id="APNKRIT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990430">
        <property type="term" value="F:extracellular matrix protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03600">
        <property type="entry name" value="CLECT_thrombomodulin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000406">
        <property type="entry name" value="CD248 molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000489">
        <property type="entry name" value="CD248 molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000061">
        <property type="entry name" value="CD248 molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF4">
        <property type="entry name" value="ENDOSIALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021284277" description="Endosialin" evidence="17">
        <location>
            <begin position="18"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="18">
        <location>
            <begin position="30"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="560"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="753"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="605"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="776" mass="82661" checksum="D51C938F448CD531" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBG7</accession>
    <name>A0A4W2CBG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBG7"/>
    <dbReference type="SMR" id="A0A4W2CBG7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016882.1">
        <property type="protein sequence ID" value="ENSBIXP00000008963.1"/>
        <property type="gene ID" value="ENSBIXG00000014679.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20188">
        <property type="entry name" value="T-box_TBX2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000003">
        <property type="entry name" value="T-box transcription factor TBX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022582">
        <property type="entry name" value="TBX2/3_TAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048387">
        <property type="entry name" value="TBX2_3_RD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF91">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20627">
        <property type="entry name" value="TBX2-3_RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12598">
        <property type="entry name" value="TBX2-3_TAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="107"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="335"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="673"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="673"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="728" mass="77551" checksum="6C014DD2336C6138" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCW5</accession>
    <name>A0A4W2CCW5_BOBOX</name>
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        <submittedName>
            <fullName evidence="10">Tubulin epsilon 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TUBE1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCW5"/>
    <dbReference type="STRING" id="30522.A0A4W2CCW5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019768.1">
        <property type="protein sequence ID" value="ENSBIXP00000010766.1"/>
        <property type="gene ID" value="ENSBIXG00000016190.1"/>
    </dbReference>
    <dbReference type="OMA" id="KRAHLHH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="epsilon_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000007">
        <property type="entry name" value="tubulin epsilon chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000017">
        <property type="entry name" value="Tubulin epsilon chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004057">
        <property type="entry name" value="Epsilon_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01519">
        <property type="entry name" value="EPSLNTUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="8">
        <location>
            <begin position="133"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="9">
        <location>
            <begin position="363"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="559" mass="61987" checksum="8D848FDE55898854" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CG35</accession>
    <name>A0A4W2CG35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CG35"/>
    <dbReference type="SMR" id="A0A4W2CG35"/>
    <dbReference type="STRING" id="30522.A0A4W2CG35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006095.1">
        <property type="protein sequence ID" value="ENSBIXP00000005267.1"/>
        <property type="gene ID" value="ENSBIXG00000000908.1"/>
    </dbReference>
    <dbReference type="OMA" id="HKARCWD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030893">
        <property type="term" value="C:meiotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.20:FF:000001">
        <property type="entry name" value="Structural maintenance of chromosomes 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000564">
        <property type="entry name" value="Structural maintenance of chromosomes 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1620:FF:000001">
        <property type="entry name" value="Structural maintenance of chromosomes 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000562">
        <property type="entry name" value="Structural maintenance of chromosomes protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF147">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="513"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="161"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="263"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="372"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="671"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="755"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1235" mass="143984" checksum="451491EEBC8ED843" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGV7</accession>
    <name>A0A4W2CGV7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CE022 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C20H5orf22</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0489 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGV7"/>
    <dbReference type="STRING" id="30522.A0A4W2CGV7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020020.1">
        <property type="protein sequence ID" value="ENSBIXP00000010939.1"/>
        <property type="gene ID" value="ENSBIXG00000016329.1"/>
    </dbReference>
    <dbReference type="OMA" id="RIFWHLE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024131">
        <property type="entry name" value="UPF0489"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13225">
        <property type="entry name" value="MISEXPRESSION SUPPRESSOR OF RAS 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13225:SF3">
        <property type="entry name" value="UPF0489 PROTEIN C5ORF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12640">
        <property type="entry name" value="UPF0489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49992" checksum="2E4D71F8253B58D6" modified="2019-09-18" version="1">MSESGGQRARLRRYSELPVWVVEDHQEVLPFIYRAIGSKHLPASNISFVHFDSHPDLLIPVDMPADTVFDKETLFGELSIENWIMPAVYAGHFSHVVWLHPTWAQQIREGRHHFLVGKDTSTTTIRVTSTDHYFLSDGLYVTEDLLENPKPLQLDVIMVKPYKLCHSQGENDAVSSAKKPKLALEDSENTASANGDSCAEGLGKDTVTQTRDHTCLQPSCSCSSESHECQTAVSTGEILEILEKRDAFVLDIDLDFFSVKNPFKEMFTQEEYKILQELYQFKKPGSNLTEEDLVDCVDNRIHQLEDLEAAFADLCDGDDEETVQKWASNPGMELLVPLVQSLKKRMEAPDYEMVHQAGLTCDYSELPHHISTEQEIEHLIQSLHTVLKNFPKPTLVTIARSSLDDYCPSEQVETIQEKVLSVLHSLYGALDIHLMYSAESTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHL3</accession>
    <name>A0A4W2CHL3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OTU deubiquitinase with linear linkage specificity like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">OTULINL</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family. Otulin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375907.1">
        <property type="nucleotide sequence ID" value="XM_027520106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHL3"/>
    <dbReference type="STRING" id="30522.A0A4W2CHL3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020486.1">
        <property type="protein sequence ID" value="ENSBIXP00000011228.1"/>
        <property type="gene ID" value="ENSBIXG00000002576.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054572.1">
        <property type="protein sequence ID" value="ENSBIXP00005000263.1"/>
        <property type="gene ID" value="ENSBIXG00005030429.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878925"/>
    <dbReference type="CTD" id="54491"/>
    <dbReference type="GeneTree" id="ENSGT00390000009802"/>
    <dbReference type="OMA" id="NDRHYHV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023235">
        <property type="entry name" value="FAM105"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023236">
        <property type="entry name" value="OTULINL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662:SF1">
        <property type="entry name" value="INACTIVE UBIQUITIN THIOESTERASE OTULINL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662">
        <property type="entry name" value="OTU DEUBIQUITINASE WITH LINEAR LINKAGE-SPECIFICITY A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16218">
        <property type="entry name" value="Peptidase_C101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02055">
        <property type="entry name" value="PROTEINF105"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02056">
        <property type="entry name" value="PROTEINF105A"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="354" mass="41866" checksum="DB4EE02B131542C7" modified="2019-09-18" version="1">MAARKSPAPERDRPRNHASGNDQVHSWTLVTSQALDTAWRVVKGSVTLAVSLLAAILSYFRRLHMHLGHRLKWWIGYLQRRFKRNLSVEAEVDLLSYCAREWKGETPHAKLMRKAYEELFWRRHIKCVRQVRRDNYDALRSVLFQIFSRGLAFPSWMKEKDIVKLPEKLLFSQGCNWIQQYSFGPEKYTGSNVFGKLRKCVELLKTQWTEFSGIKDYHKRGSMCNILFSDALLECKLYEALKFIMLYQVTEVYEQMKTKKVIPSLFRLLFTRETSSDPLSFMMNHLNSVGDTCGLEQIDMFILGYSLEVKIKVFRLFKFNSRDFEVCYPEESLREWPEISLLTENDRHYHIPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMC7</accession>
    <name>A0A4W2CMC7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="18">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">USP37</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with FZR1/CDH1. Interacts with CDT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="18"/>
    <dbReference type="RefSeq" id="XP_027369212.1">
        <property type="nucleotide sequence ID" value="XM_027513411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMC7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025079.1">
        <property type="protein sequence ID" value="ENSBIXP00000014275.1"/>
        <property type="gene ID" value="ENSBIXG00000007917.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050124.1">
        <property type="protein sequence ID" value="ENSBIXP00005040547.1"/>
        <property type="gene ID" value="ENSBIXG00005023268.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907816"/>
    <dbReference type="CTD" id="57695"/>
    <dbReference type="GeneTree" id="ENSGT00940000158091"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd13312">
        <property type="entry name" value="PH_USP37_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.180:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000040">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000287">
        <property type="entry name" value="Ubiquitin specific peptidase 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.180">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 26/29/37, pleckstrin homology-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032069">
        <property type="entry name" value="USP37-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038093">
        <property type="entry name" value="USP37-like_PH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF686">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16674">
        <property type="entry name" value="UCH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 18" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 18" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="21">
        <location>
            <begin position="343"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="111"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="648"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="699"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="777"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="661"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="754"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="959" mass="108096" checksum="26F5011C64514C56" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CP70</accession>
    <name>A0A4W2CP70_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ING4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396954.1">
        <property type="nucleotide sequence ID" value="XM_027541153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CP70"/>
    <dbReference type="SMR" id="A0A4W2CP70"/>
    <dbReference type="STRING" id="30522.A0A4W2CP70"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015661.1">
        <property type="protein sequence ID" value="ENSBIXP00000008136.1"/>
        <property type="gene ID" value="ENSBIXG00000014047.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027390.1">
        <property type="protein sequence ID" value="ENSBIXP00005016160.1"/>
        <property type="gene ID" value="ENSBIXG00005019901.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892363"/>
    <dbReference type="CTD" id="51147"/>
    <dbReference type="GeneTree" id="ENSGT00940000159033"/>
    <dbReference type="OMA" id="PIYITPQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045111">
        <property type="term" value="C:intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    <sequence length="248" mass="28459" checksum="DA0C4EBC3741B4BC" modified="2019-09-18" version="1">MAAGMYLEHYLDSIENLPFELQRNFQLMRDLDQRTEDLKAEIDKLASEYMSSARSRSSEEKLALLRQIQEAYGKCKEFGDDKVQLAMQTYEMVDKHIRRLDTDLARFEADLKEKQIESSDYDSSSSKGKKSRTQKEKKAARARSKGKNSDEEAPKAAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCSIEWFHFACVGLTTKPRGKWFCPRCSQERKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:scavenger receptor activity"/>
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        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
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    </dbReference>
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        <property type="entry name" value="EGFLAMININ"/>
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        <property type="match status" value="7"/>
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    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="1"/>
            <end position="36"/>
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        <location>
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            <begin position="786"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="843" mass="88799" checksum="80FB153B05F9D550" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQD6</accession>
    <name>A0A4W2CQD6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">CSN12-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PCID2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and transcription-associated genomic instability. Blocks the activity of the SRCAP chromatin remodeling complex by interacting with SRCAP complex member ZNHIT1 and inhibiting its interaction with the complex. This prevents the deposition of histone variant H2AZ1/H2A.Z at the nucleosomes of key lymphoid fate regulator genes which suppresses their expression and restricts lymphoid lineage commitment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, 2 copies of ENY2, PCID2, SEM1/DSS1, and either centrin CETN2 or centrin CETN3. The TREX-2 complex also associates with ALYREF/ALY and with the nucleoporin NUP153. Interacts with BRCA2. Interacts with SRCAP chromatin remodeling complex component ZNHIT1; the interaction results in inhibition of SRCAP complex activity, preventing the deposition of histone variant H2AZ1/H2A.Z to lymphoid fate regulator genes and restricting lymphoid lineage commitment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027413828.1">
        <property type="nucleotide sequence ID" value="XM_027558027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQD6"/>
    <dbReference type="STRING" id="30522.A0A4W2CQD6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046981.1">
        <property type="protein sequence ID" value="ENSBIXP00000015382.1"/>
        <property type="gene ID" value="ENSBIXG00000003581.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031969.1">
        <property type="protein sequence ID" value="ENSBIXP00005019235.1"/>
        <property type="gene ID" value="ENSBIXG00005022462.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902587"/>
    <dbReference type="CTD" id="55795"/>
    <dbReference type="GeneTree" id="ENSGT00390000001101"/>
    <dbReference type="OMA" id="INRMFTL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000146">
        <property type="entry name" value="PCI domain-containing protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="2" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="210"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="399" mass="45954" checksum="F68B363C5FCF9986" modified="2019-09-18" version="1">MAHITINQYLQQVCEAIDTRDGASLAELVSFKHPHVANPRLQMASPEEKCQQVLEPPYDEMFAAHLRCTYAVGNHDFIEAYKCQTVIVQSFLRAFQAHKEENWALPVMYAVALDLRIFANNADQQLVKKGKSKVGDMLEKAAELLMGCFRVCASDTRAGIEDSKKWGMLFLVNQLFKIYFKINKLHLCKPLIRAIDSSNLKDDYSTAQRVTYRYYVGRKAMFDSDFKQAEEYLSFAFEHCHRSSQKNKRMVLIYLLPVKMLLGHMPTIELLRKYHLMQFAEVTRAVSEGNLLLLNEALAAHETFFIRCGIFLILEKLKIITYRNLFKKVYLLLKTHQLSLDAFLVALKFMQVEDVDIAEVQCILANLIYMGHIKGYISHQHQKLVVSKQNPFPPLSTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQX1</accession>
    <name>A0A4W2CQX1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="10">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Carbohydrate kinase domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NAXD</name>
        <name evidence="10" type="synonym">CARKD</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="10"/>
    <dbReference type="RefSeq" id="XP_027413772.1">
        <property type="nucleotide sequence ID" value="XM_027557971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQX1"/>
    <dbReference type="STRING" id="30522.A0A4W2CQX1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047074.1">
        <property type="protein sequence ID" value="ENSBIXP00000015587.1"/>
        <property type="gene ID" value="ENSBIXG00000003630.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033916.1">
        <property type="protein sequence ID" value="ENSBIXP00005020492.1"/>
        <property type="gene ID" value="ENSBIXG00005007733.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902564"/>
    <dbReference type="CTD" id="55739"/>
    <dbReference type="GeneTree" id="ENSGT00390000000917"/>
    <dbReference type="OMA" id="WRAAYHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000013">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="345" mass="36103" checksum="0F87C2A4B48BE973" modified="2019-09-18" version="1">MAVRAGAAVAGAAVVAVLSAALALYGPPLDAVLKRAFSLHKAHSVKDMESILQLVRSVVPALTTKKHKGQDGRIGVVGGCREYTGAPYFAAISALKVGADLSHVFCTQEAAPVIKAYSPELIVHPVLDSPEAVRDVEQWLPRLHALVVGPGLGRDDALLENVKGILEASKARGIPVVIDADGLWLIAQQPALIQGYRKAVLTPNHVEFGRLSEAVLGVPLDGGDRHGAVLRLSQALGNVTVVQKGEQDVISDGEQVLECSQEGSGRRCGGQGDLLSGSLGVLAHWALRAGPQKTGGPSPLLVAAFGACALTRQCSQQAFQKYGRATTTSDMVAEVGPAFRRLFEA</sequence>
</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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    <sequence length="440" mass="50294" checksum="EC2DD3EC6CC6CF6B" modified="2019-09-18" version="1">MNKASGGDGIPVELFQILKDDAVKALHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALISHASKGMLKIPQARLQQYVNRELPDVQAGFRKDRGTRYQIANIHWIIEKAREFQKNIYFCFIDYAKIFDCVDPNKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEETQAGIKIAGRNISNLRYADDTTLLAESEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRHLLLGRKVVNNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAKCRRIDAFELWYWRSLLRVPWTARRSNQSILKEISPGISLEGMMLSLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTR0</accession>
    <name>A0A4W2CTR0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="17">Delta and Notch-like epidermal growth factor-related receptor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="22" type="primary">DNER</name>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subunit">
        <text evidence="16">Interacts with AP1G1. Interacts with NOTCH1.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:neuronal cell body"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:central nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-like_CS"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
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        <property type="entry name" value="ATTRACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19330">
        <property type="entry name" value="DNER_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
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</entry>
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</entry>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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    <sequence length="520" mass="60068" checksum="01163675DF14B5E2" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="7">Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity. Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides.</text>
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        <text evidence="8">Interacts with AARS; the interaction is direct.</text>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </feature>
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        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00062703"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="364" mass="39793" checksum="01A978E3412B62BC" modified="2019-09-18" version="1">MAAPADPRRLCRLVQDGRLCTLREELQAAGHAGCAGPAGDTLLHCAARHGRRDVLAYLVEAWDLDIEAANRDYKRPLHEAASMGHRNCVRYLLGRRAAVDCLKKADWTPLMMACTRRNLEVIRDLVEHGANPLLKNKDGWNSFHIASREGDPLILQYLLAVCPSAWRTESKIGRTPLHTAAMHGCLEAVEVLLQRCQYEPDCRDRCGLTPFMDAVQCGHTDVARLLLKKHKACVSAEDSLGAQAIHRAAVTGQDEAIRFLVSELGVDVDARAGSTHLTALHFAAKEGHVSTVQTLLSLGADINSKDERNRSAYSALNVSSVRDPPPPQPPRPPDREDPRLGCDSAPGPPRPPASFGSVEEQSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6B4</accession>
    <name>A0A4W2D6B4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein aristaless-like 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ALX4</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Binds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D6B4"/>
    <dbReference type="SMR" id="A0A4W2D6B4"/>
    <dbReference type="STRING" id="30522.A0A4W2D6B4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025071.1">
        <property type="protein sequence ID" value="ENSBIXP00000014268.1"/>
        <property type="gene ID" value="ENSBIXG00000018992.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022819.1">
        <property type="protein sequence ID" value="ENSBIXP00005013115.1"/>
        <property type="gene ID" value="ENSBIXG00005017263.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159662"/>
    <dbReference type="OMA" id="PCYGKDN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071837">
        <property type="term" value="F:HMG box domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042733">
        <property type="term" value="P:embryonic digit morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035115">
        <property type="term" value="P:embryonic forelimb morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035116">
        <property type="term" value="P:embryonic hindlimb morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001942">
        <property type="term" value="P:hair follicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060021">
        <property type="term" value="P:roof of mouth development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000127">
        <property type="entry name" value="homeobox protein aristaless-like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF322">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="198"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="17">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
        <location>
            <begin position="200"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="58"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="397" mass="42481" checksum="1B0920B612112939" modified="2019-09-18" version="1">MNAETCVSYCESPAAAMDAYYSPVSQSREGSSPFRAYPGGDKFSTTFLSAAAKGQGFGDAKSRARYGAGQQDPAAPLESGAGARGSFSKFQPQPPAPQPQPPAPQPHLYLQRGACKTPPDGSLKLQEGGGGHNAALQVPCYAKESSLGEPELPPDSDTVGMDSSYLSVKEAGVKGPQDRASADLPSPMEKADSESNKGKKRRNRTTFTSYQLEELEKVFQKTHYPDVYAREQLAMRTDLTEARVQVWFQNRRAKWRKRERFGQMQQVRTHFSTAYELPLLTRAENYAQIQNPSWIGNNGAASPVPACVVPCDPVPACMSPHAHPPGSGASGVTDFLSVSGAGSHVGQTHMGSLFGAAGLSPGLNGYELNGEPDRKTSSIAALRMKAKEHSAAISWAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D707</accession>
    <name>A0A4W2D707_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U4/U6 small nuclear ribonucleoprotein Prp3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-mRNA-splicing factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PRPF3</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Interacts directly with PRPF4. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Interacts with SART3; the interaction is direct and recruits the deubiquitinase USP4 to PRPF3. Interacts with PRPF19. Interacts ('Lys-63'-linked polyubiquitinated) with PRPF8 (via the MPN (JAB/Mov34) domain); may stabilize the U4/U6-U5 tri-snRNP complex. Interacts with ERCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D707"/>
    <dbReference type="STRING" id="30522.A0A4W2D707"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036512.1">
        <property type="protein sequence ID" value="ENSBIXP00000021716.1"/>
        <property type="gene ID" value="ENSBIXG00000024750.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051980.1">
        <property type="protein sequence ID" value="ENSBIXP00005023632.1"/>
        <property type="gene ID" value="ENSBIXG00005026388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011497"/>
    <dbReference type="OMA" id="NPQHRFK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.10:FF:000003">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
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    </dbReference>
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        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI domain"/>
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        <property type="entry name" value="PWI"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PWI" evidence="12">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="89"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016514"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032955"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="699" mass="79365" checksum="3C2F72EA850C553A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D827</accession>
    <name>A0A4W2D827_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113891821</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="309" mass="35158" checksum="C8B711DC9B95D93E" modified="2019-09-18" version="1">MESNQSWVTEFILVGFHLSAKMEKLLFWIFSLFYIFSLLANGMILGLICLDLRLHTPMYFFLSHLAIIDMSYASNNVPKMMANLANKNRTISFVPCILQTFLYLGFAATECLILMVMSYDRFVAICHPLQYTVIMNWRACRVLATTSWACGFILALAHAVLLLRLPFCGPWEVNHLFCEILSVLKLACVDTWINQVVLLAASIFVLVGPLCLMLVSYIHILCAVLKIQSKEGRRKAFSICSSHLCVVGLFFGIAMLVYMVPDSNQREEEEKILSLFHSLFNPFLNPLIYSLRNAQVKGALYRALQKRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBK6</accession>
    <name>A0A4W2DBK6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinesin-like protein KIF26A/B helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBK6"/>
    <dbReference type="STRING" id="30522.A0A4W2DBK6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027920.1">
        <property type="protein sequence ID" value="ENSBIXP00000016136.1"/>
        <property type="gene ID" value="ENSBIXG00000020417.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057090">
        <property type="entry name" value="HTH_KIF26A_B_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21608">
        <property type="entry name" value="KINESIN-LIKE PROTEIN CG14535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21608:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF26B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23081">
        <property type="entry name" value="HTH_KIF26A_B_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin-like protein KIF26A/B helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="284" mass="28857" checksum="501F8D6D7F88235B" modified="2019-09-18" version="1">MNSIAGNKERLAVSTRGKKYGVNEVCSPTKPAAPFSPESWYRKAYEESRAGSRPTPEGAGSALGSSGTPSPGSGTSSPSSFTGSPGPASPGIGTSSPGSLGGSPGFGTGSPGSGSGGGSSPGSDRGVWCENCNARLVELKRQALKLLLPGPFPGKVSAAGDGTGLAGHSCARLAAAGGGGTCSATRCSVADGSLAPPGQLWAAGREGGDPARLRRETFLRRIAWMVRAQFQASLSTWVAQVILPASLHFPECPFSAPCTRPAIRMDPRSATFPRDARPPRVHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEB6</accession>
    <name>A0A4W2DEB6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEB6"/>
    <dbReference type="SMR" id="A0A4W2DEB6"/>
    <dbReference type="STRING" id="30522.A0A4W2DEB6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029442.1">
        <property type="protein sequence ID" value="ENSBIXP00000017137.1"/>
        <property type="gene ID" value="ENSBIXG00000021162.1"/>
    </dbReference>
    <dbReference type="OMA" id="MHNVYGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000046">
        <property type="entry name" value="Glucosidase alpha, neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000023">
        <property type="entry name" value="neutral alpha-glucosidase AB isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF60">
        <property type="entry name" value="NEUTRAL ALPHA-GLUCOSIDASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose mutarotase-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="356"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="692"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="914" mass="103485" checksum="3C79E136C3AB6479" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEL7</accession>
    <name>A0A4W2DEL7_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2DEL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029607.1">
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        <property type="gene ID" value="ENSBIXG00000021251.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MRI"/>
    </dbReference>
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        <property type="entry name" value="CELL CYCLE REGULATOR OF NON-HOMOLOGOUS END JOINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14566:SF0">
        <property type="entry name" value="CELL CYCLE REGULATOR OF NON-HOMOLOGOUS END JOINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15325">
        <property type="entry name" value="MRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="152" mass="16657" checksum="EF1711DBFB339523" modified="2019-09-18" version="1">MEDLKSENKKRVLPTWMTAQVAEKRAPQVKTPKRTPATVPAAAARFPAMRTVYCMNEAEVVDVALGILIEDRKQEKPLEPPPLAGADKPERSPASPAPPSPSPRWSRSEDSEEEDRGADGPAPGPAGSDTACSRSLEEDQDELKYVREIFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFG1</accession>
    <name>A0A4W2DFG1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nectin-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nectin cell adhesion molecule 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Poliovirus receptor-related protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">NECTIN4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384011.1">
        <property type="nucleotide sequence ID" value="XM_027528210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFG1"/>
    <dbReference type="SMR" id="A0A4W2DFG1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041318.1">
        <property type="protein sequence ID" value="ENSBIXP00000024914.1"/>
        <property type="gene ID" value="ENSBIXG00000027042.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883966"/>
    <dbReference type="CTD" id="81607"/>
    <dbReference type="OMA" id="MFYESNG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:symbiont entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07704">
        <property type="entry name" value="IgC1_2_Nectin-3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05888">
        <property type="entry name" value="IgV_1_Nectin-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000560">
        <property type="entry name" value="Nectin cell adhesion molecule 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000627">
        <property type="entry name" value="Nectin cell adhesion molecule 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001007">
        <property type="entry name" value="nectin-4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033320">
        <property type="entry name" value="IgC1_2_Nectin-3-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF11">
        <property type="entry name" value="NECTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021185525" description="Nectin-4" evidence="20">
        <location>
            <begin position="32"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="45"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="148"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="262"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="400"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="510" mass="55398" checksum="637D048D5B30118D" modified="2019-09-18" version="1">MPLSLGAEMWGPAAWLLLLLLLASFTGQRLAGELETSDLVTVVLGQDAKLPCFYRGDPGEQVEQVAWARVDAGEGGRELALLNSKYGLHVSSAYEGRVEQPPPPRNPLDGAVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPPLEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTASHRSFTHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILQVAFLAEASVRGLEDRKLWQVGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRAEGDTLGFPTLTPEHSGTYVCRVSNALSSRDSQVVVDVLDPEDAPGKQVDLVSASVVVVGVIAALLFCLLVVVVVLMSRYHRRKAQQMTQKYEEELTLTRENSIRRLHSHHSDPRNQPEESVGLRAEGHPDSLKDNSSCSVMSEEPEGRSYSTLTTVREIETQTELPSPGPGRAEEEEDRDEGIKQAMNHFVQENGTLRAKPTGNGIYINGRGHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DID6</accession>
    <name>A0A4W2DID6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113893692</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DID6"/>
    <dbReference type="STRING" id="30522.A0A4W2DID6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043048.1">
        <property type="protein sequence ID" value="ENSBIXP00000026071.1"/>
        <property type="gene ID" value="ENSBIXG00000028159.1"/>
    </dbReference>
    <dbReference type="OMA" id="LFHKQMK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF29">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="36010" checksum="C2C10E04D7BFABAF" modified="2019-09-18" version="1">MRNNTEIRDFILLGLSDDPQLQVVVFVFLLITYMLSITGNLTIITLTLLDIHLQTPMYFFLRSFSILEVSFTTVTIPKFLSTIITGDKTISFNNCMTQLFFFIFLGVTEFYLLVAMSYDRYIAICKPLHYMTIMNHRVCTLLVLASWLTSFLIIFPLLMFFIQLDYCKSKVINHFTCDYFPLLYLSCSDTKFLEILGFSCAVFILLFTLALIIMSYTYVIRTILRIPSTTQRTKAFSTCSSHMIVISISYGSCIFMYVNPSAKDRVSLSKGVAVLNTSVAPMLNPFIYTLRNQQVKRAFMDMARKTMFFSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIP9</accession>
    <name>A0A4W2DIP9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anaphylatoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIP9"/>
    <dbReference type="STRING" id="30522.A0A4W2DIP9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031947.1">
        <property type="protein sequence ID" value="ENSBIXP00000018739.1"/>
        <property type="gene ID" value="ENSBIXG00000004376.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.91.20">
        <property type="entry name" value="Anaphylotoxins (complement system)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018081">
        <property type="entry name" value="Anaphylatoxin_comp_syst"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01821">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47686">
        <property type="entry name" value="Anaphylotoxins (complement system)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Anaphylatoxin-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="82" mass="9406" checksum="D6DB7EAA30561253" modified="2019-09-18" version="1">TAFEKTEEETSTFKHPLIRKCCSGGAHRPEETCEQRAARITISQKCQSFHWCQTLLFRAPKSLQMVTAAMKLKDAYSLEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIQ5</accession>
    <name>A0A4W2DIQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040160.1">
        <property type="protein sequence ID" value="ENSBIXP00000024179.1"/>
        <property type="gene ID" value="ENSBIXG00000026423.1"/>
    </dbReference>
    <dbReference type="OMA" id="WTNQEAE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="88"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="376" mass="43095" checksum="2DAA8D20F0F40117" modified="2019-09-18" version="1">MVWTNQEAEDIKRWQEYTEELYKKDLHDPYNHDGVITHLEPDILECEVRWALESITMNKASGGDGIPVELFQILKDNAVKVLHSICQQIWKTQQWPQDWKRSVFIPILKKGNAKECSNYRTIALISHTSKVMLKILQARLQQYLNREFPDVQAGFRKGRGTRDQIANICWIIKKAREFQKNIYFCFIDYAKAFVCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATIRTGHGTTDWFQIGKGVHQGCIFSPCLFNLYAEYIMRNAGLDEAQAGIKIAGRNINNLRYADDTTLVAESEEELKSLLMKVKEESENVGLKLNVQKTKIMASGPITSWEIDGETVKTVSDFILGAPKSLQMVTAAMKLKDTYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJF1</accession>
    <name>A0A4W2DJF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113883606</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJF1"/>
    <dbReference type="SMR" id="A0A4W2DJF1"/>
    <dbReference type="STRING" id="30522.A0A4W2DJF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053369.1">
        <property type="protein sequence ID" value="ENSBIXP00000026473.1"/>
        <property type="gene ID" value="ENSBIXG00000029965.1"/>
    </dbReference>
    <dbReference type="OMA" id="HTLLMAN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35197" checksum="EA38F2821F6326C2" modified="2019-09-18" version="1">MVEANQSRVSEFLLLGLSRQPQQQQQLLFLLFLTMYLATVLGNLLILLAISMDSHLHIPMYFFLCNLSFVDTYFSSTTVPKVLANHVLRSHTISFSECLTQMYFVFMFMDMDNFLLAVIAYDRFVAVCHPLHYSAKMTPQLCALLVTGSWVTASLDMLLHTLLMARLSFCADNAIPHFFCDVTPLLKLSCSDTHLNEVVILTEGALIMITPFVCILVSYVLITCAVLRIPSTKGRWKAFSTCGSHLAVVFLFYGTIIFLYFNPLSSHAAETDVAAGVMFSVVTPMLNPFIYSLRNQDIKGALGKVIAMKFFSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DN26</accession>
    <name>A0A4W2DN26_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chitinase-3-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DN26"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034311.1">
        <property type="protein sequence ID" value="ENSBIXP00000020309.1"/>
        <property type="gene ID" value="ENSBIXG00000004699.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000047">
        <property type="entry name" value="Chitinase-3-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF82">
        <property type="entry name" value="CHITINASE-3-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="GH18" evidence="16">
        <location>
            <begin position="37"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="402" mass="44853" checksum="9DE6631B066F69D0" modified="2019-09-18" version="1">MSIPEKLTRDMGVIAMDQKSFWEGLVVLLLLQEGCAYKLVCYFTNWSQDRQEPGKFTLENTDPFLCSHLIYSFASISNNKVIIKDKNEAKLYQTINSLKTKNPKLKILLSIGGYLFGSKGFHPVVESSSSTLKFVNSVILFLRNHNFDGLDISWIYPNVKDNTHLTVLIHKLAEAFQQDFVKSTKERLLLTAGVSAGRQMIDNSYQIKELAKDLDFINLLSFDFHGSWEKPLVTGHNSPLRKGQLDRGTSSYYNVEYAVGYWINKGMPAEKVVMGIPTYGRSFTLASAETAVGAPASGPGAAGPITKSSGFLAYYEICQFLQGAKITRLQDQQVPYAVKGNQWVGYDDVESVETKVQFLKNLNLGGAMIWSIDMDDFTGKLCSQGPYPLVQAVKRSLGSLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNS3</accession>
    <name>A0A4W2DNS3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IKBKB interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IKBIP</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027397880.1">
        <property type="nucleotide sequence ID" value="XM_027542079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DNS3"/>
    <dbReference type="STRING" id="30522.A0A4W2DNS3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042621.1">
        <property type="protein sequence ID" value="ENSBIXP00000025784.1"/>
        <property type="gene ID" value="ENSBIXG00000005849.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024288.1">
        <property type="protein sequence ID" value="ENSBIXP00005036587.1"/>
        <property type="gene ID" value="ENSBIXG00005018134.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892767"/>
    <dbReference type="CTD" id="121457"/>
    <dbReference type="GeneTree" id="ENSGT00500000045001"/>
    <dbReference type="OMA" id="LTLQMFN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024152">
        <property type="entry name" value="Inh_kappa-B_kinase-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21734">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21734:SF11">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="180"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="376" mass="42863" checksum="73902D2C1EDB3CD7" modified="2019-09-18" version="1">MSEVKSRKKSGTKGAPAEPGKRNEGGKSPEARGGGGRGWADPRTGVSLLSLGTCLGLAWFVFQQSEKFAKVENQYQLLKMETSEFQGLQSKISLISEKLASTESILQEATSSMSLVTQFEQEVATLQRIMHDIQTSEEMLTQKMKSLNEKFQNVTDFWKRSLEEMNVNTDVFKSESKHIHSQVTVQINSAEQEIKLLTERLKDLEDSTVRNIRTIQRQEEEDLLRVEEQLGSDTKAVEKLEEEQHALFARDEDLTNKLSSYEPKVEECKTHLPTIESAVRSVLRVSQDLIGTEKKMEDLTLQMFNMEDDMLKAVSEIMEMQKTLEGIQYDNSILKMQNDLDVLKGKVHDFIVYSSTREKGTPEEYNLENKEIDSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DPX6</accession>
    <name>A0A4W2DPX6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tryptase</fullName>
            <ecNumber evidence="8">3.4.21.59</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.</text>
            <dbReference type="EC" id="3.4.21.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.59" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DPX6"/>
    <dbReference type="SMR" id="A0A4W2DPX6"/>
    <dbReference type="STRING" id="30522.A0A4W2DPX6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009985.1">
        <property type="protein sequence ID" value="ENSBIXP00000028541.1"/>
        <property type="gene ID" value="ENSBIXG00000009781.1"/>
    </dbReference>
    <dbReference type="OMA" id="RIFIACT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="123"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="385" mass="41923" checksum="2EF222A34F4DE66E" modified="2019-09-18" version="1">MWGSGVGVRNLGLEMGRFLGKKPVMRVLAAASCFSHWTLMFPGEGARPKEDYAMSLATPEASSASGQLKSLQVPVPTVAPVPPTPTAKETVGSSQTPVLEGLPPLGTSPLLSQGACGHRTLGIIGGKPAPERKWPWQVSLQLRGRHRCGGSLIAPQWVLTAAHCVEHFREFTVMMGTTYLYSHCKTTVVVPVKHIKSHKDFDWNLTPNDIALLQLAHSVNYSAYIQPVCLPRKNFEVRPGTQCWITGWGRTLEFDPASMSPKLQEAEQLIIPLKQCAVMVEKTSNKSGNRVQKGMVCAQNIKSEGPCRGDSGSPLVCQFQTSWIQVGIVSWGDRCGLKEVPAVYTDVSFYKDWITARMSQASGLDSGFLILLLCLLLPLGILATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQ95</accession>
    <name>A0A4W2DQ95_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase</fullName>
            <ecNumber evidence="9">3.6.1.62</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DCP1A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQ95"/>
    <dbReference type="SMR" id="A0A4W2DQ95"/>
    <dbReference type="STRING" id="30522.A0A4W2DQ95"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053803.1">
        <property type="protein sequence ID" value="ENSBIXP00000026313.1"/>
        <property type="gene ID" value="ENSBIXG00000030246.1"/>
    </dbReference>
    <dbReference type="OMA" id="SASRFKM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000097">
        <property type="entry name" value="Putative mRNA-decapping enzyme 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031953">
        <property type="entry name" value="mRNA_decap_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF4">
        <property type="entry name" value="MRNA-DECAPPING ENZYME 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16741">
        <property type="entry name" value="mRNA_decap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA-decapping enzyme C-terminal" evidence="12">
        <location>
            <begin position="538"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="320"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="579" mass="62767" checksum="1E0220593DF25614" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQD3</accession>
    <name>A0A4W2DQD3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Relaxin receptor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Leucine-rich repeat-containing G-protein coupled receptor 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Relaxin family peptide receptor 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Receptor for relaxin. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. May also be a receptor for Leydig insulin-like peptide (INSL3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQD3"/>
    <dbReference type="STRING" id="30522.A0A4W2DQD3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000094.1">
        <property type="protein sequence ID" value="ENSBIXP00000026669.1"/>
        <property type="gene ID" value="ENSBIXG00000029623.1"/>
    </dbReference>
    <dbReference type="OMA" id="NISTQMF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000023">
        <property type="entry name" value="Relaxin family peptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000014">
        <property type="entry name" value="Relaxin family peptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000207">
        <property type="entry name" value="Relaxin family peptide receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000300">
        <property type="entry name" value="Relaxin family peptide receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008112">
        <property type="entry name" value="Relaxin_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RELAXIN RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="RELAXINR"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
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    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021266519" description="Relaxin receptor 2" evidence="19">
        <location>
            <begin position="17"/>
            <end position="753"/>
        </location>
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        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="453"/>
            <end position="477"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="497"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="538"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="592"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="635"/>
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            <begin position="670"/>
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        <location>
            <begin position="431"/>
            <end position="690"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00080271"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00081645"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026669.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="753" mass="85542" checksum="2C0B88E94E47FE28" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQT4</accession>
    <name>A0A4W2DQT4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPRY domain-containing SOCS box protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SPSB3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as CGAS and SNAI1. The ECS(SPSB3) complex catalyzes 'Lys-48'-linked ubiquitination of nuclear CGAS in cycling cells, leading to its degradation. Recognizes and binds nucleosome-bound CGAS: ubiquitination and degradation of nuclear CGAS during G1 and G2 phases is required to promote low intranuclear CGAS abundance before the next mitotic cycle. The ECS(SPSB3) complex also mediates ubiquitination and degradation of phosphorylated SNAI1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Substrate-recognition component of the ECS(SPSB3) complex, composed of SPSB3, CUL5, ELOB, ELOC and RNF7/RBX2. Interacts with MET.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPSB family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027526875.1"/>
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        <property type="nucleotide sequence ID" value="XM_027526876.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000026844.1"/>
        <property type="gene ID" value="ENSBIXG00000029303.1"/>
    </dbReference>
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    <dbReference type="UniPathway" id="UPA00143"/>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SPRY domain-containing SOCS box protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
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        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035754">
        <property type="entry name" value="SPRY_SPSB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF5">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
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    </dbReference>
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        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="84"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014684"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056941"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="345" mass="38181" checksum="CFFC28BABD5F78EA" modified="2019-09-18" version="1">MARRPRSSRAWHFVLSAARRDADARAVALAGTANWGYDSDGQHSDSDSDPECASLPSSIPSAVPVTGESFCDCDSQSEAFCGSLHTAHRGRDCRCGEEDEYFDWVWDDLNKSSATLLSCDNRKVSFHMEYSCGTAAIRGTKELGEGQHFWEIKMTSPVYGTDMMVGIGTSDVDLDKYHHTFCSLLGRDEDSWGLSYTGLLHHKGDKTSFSSRFGQGSIIGVHLDTWHGTLTFFKNRKCIGVAATQLQNKRFYPMVCSTAAKSSMKVIRSCASVTSLQYLCCFRLRQLRPGSGDTLEGLPLPPGLKQVLHHKLGWVLSMSRQPPAPSPAANGPEPRRCQRKRCRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSS6</accession>
    <name>A0A4W2DSS6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="462" mass="53851" checksum="A64B741CC5AECE89" modified="2019-09-18" version="1">MLKILQARLQQYMNHELPDVPAGCRKGRGTRDQIAKIRWIIKKAREFQKNILLYCYAKAFDCVDHNQLWKILQEMGIPDHLTCLLRNLYAAQEATVRTGHGTTDWFQIGKGVHQGCILSPCLFNFYAEYIMRNSGLEEAQAGIKIAGRNINNLRYADDTTHMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIVLGSKITADGDCSHEIKKRLLLGRKVMTNLDSIFKSRDITWPTKVCLVKAMVFPVVMYGCESWTVKKAERQRTDAFELWCWRRLLTVPWTARRSNQSILKEIRRGCGEKGTLLHCWWECKLVQPLWRTVWRFLKKLEIELPYDPAIPLLGIHTEKTRRERDTCTPMFIAALFITARTWKQPRCPSADERIRKLWYIYTMEYYSAIKKNTFESVLMMWMKLEPIIQSEVSQKEKHQYSILTHIYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DX49</accession>
    <name>A0A4W2DX49_BOBOX</name>
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        <recommendedName>
            <fullName evidence="15">ADAM metallopeptidase domain 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        </location>
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        </location>
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        <location>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="80487" checksum="291E9C42D31E5870" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXA0</accession>
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        <recommendedName>
            <fullName evidence="4">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        </location>
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            <end position="304"/>
        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031353.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="342" mass="37514" checksum="BB5FD26400653084" modified="2019-09-18" version="1">MARVLIVGAGLTGSLCAALLRREASSSLRLTVWDKAGDSGGRMTTASSPHNPQSTVDLGAQYITCTPHYAKIHQSFYDELLAGGILKPLISPIDGMVMKEGDCNFVAPHGVSSIINHYLKQSGADICFRQRVTQINLRDNKWEVSRETGSSEQFDIVVLTMPAPQILQLRGDITNLISECQRQQLESVSYSSRYALGLFYEAGTKIDVPWAGRYITSNPCIRFISIDNKKRNIESSEIGPSLVIHTTVPFGVTHLEHSTEDVQELIFKQLENIVPGLPQPVATKCQKWRHSQVTNAAANSLGQMTLHLQPFLVCGGDGFTQSNFDGCITSALCVLEALKNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXZ2</accession>
    <name>A0A4W2DXZ2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nonsense-mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with TERT, PPP2CA and SMG1. Part of a complex that contains SMG1, SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1. Not detected in complexes that contain unphosphorylated UPF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXZ2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040163.1">
        <property type="protein sequence ID" value="ENSBIXP00000024178.1"/>
        <property type="gene ID" value="ENSBIXG00000026439.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09884">
        <property type="entry name" value="PIN_Smg5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000017">
        <property type="entry name" value="protein SMG5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF39">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="11">
        <location>
            <begin position="851"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="403"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="794"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1012" mass="113212" checksum="1B4E2729F99D97AF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5P3</accession>
    <name>A0A4W2E5P3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Protein phosphatase 1B</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Protein phosphatase 2C isoform beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PPM1B</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027410945.1">
        <property type="nucleotide sequence ID" value="XM_027555144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5P3"/>
    <dbReference type="STRING" id="30522.A0A4W2E5P3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022672.1">
        <property type="protein sequence ID" value="ENSBIXP00000034652.1"/>
        <property type="gene ID" value="ENSBIXG00000017704.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049737.1">
        <property type="protein sequence ID" value="ENSBIXP00005040052.1"/>
        <property type="gene ID" value="ENSBIXG00005022600.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901154"/>
    <dbReference type="CTD" id="5495"/>
    <dbReference type="GeneTree" id="ENSGT00940000156070"/>
    <dbReference type="OMA" id="QDNRVNG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050687">
        <property type="term" value="P:negative regulation of defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032688">
        <property type="term" value="P:negative regulation of interferon-beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901223">
        <property type="term" value="P:negative regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.430:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.430">
        <property type="entry name" value="Phosphatase 2C, C-terminal domain suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012911">
        <property type="entry name" value="PP2C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036580">
        <property type="entry name" value="PP2C_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07830">
        <property type="entry name" value="PP2C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81601">
        <property type="entry name" value="Protein serine/threonine phosphatase 2C, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 24" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="26">
        <location>
            <begin position="23"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="426"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="435"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="479" mass="52768" checksum="626CE49EC0DEA429" modified="2019-09-18" version="1">MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITNNEDFRAAGKSGSALEPSVENVKNGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPKHIYFINCGDSRAVLYRSGQVCFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFIILACDGIWDVMSNEELCEFVKSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFSNAPKVSDEAMRKDSELDKYLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRHVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEENPAEQAATAASSNSDAGNTVAMQESHTESKSDLAELDSCTEDAGTKMSGEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5T4</accession>
    <name>A0A4W2E5T4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flotillin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FLOT2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECPAS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372835.1">
        <property type="nucleotide sequence ID" value="XM_027517034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5T4"/>
    <dbReference type="STRING" id="30522.A0A4W2E5T4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014649.1">
        <property type="protein sequence ID" value="ENSBIXP00000027067.1"/>
        <property type="gene ID" value="ENSBIXG00000028879.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020406.1">
        <property type="protein sequence ID" value="ENSBIXP00005011479.1"/>
        <property type="gene ID" value="ENSBIXG00005006444.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877174"/>
    <dbReference type="CTD" id="2319"/>
    <dbReference type="GeneTree" id="ENSGT00560000077232"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03399">
        <property type="entry name" value="SPFH_flotillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000003">
        <property type="entry name" value="Flotillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031905">
        <property type="entry name" value="Flotillin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF46">
        <property type="entry name" value="FLOTILLIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15975">
        <property type="entry name" value="Flot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="7">
        <location>
            <begin position="87"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="428" mass="47073" checksum="75AB2EC8A03131B8" modified="2019-09-18" version="1">MGNCHTVGPNEALVVSGGCCGSDYKQYVFGGWAWAWWCISDTQRLSLEVMTILCRCENIETSEGVPLFVTGVAQVKIMTEKELLAVACEQFLGKSVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADTKIADSKRAFELQKSAFSEEVNIKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRKKQIAVEAQEILRTDKELIATVRCPAEAEAHRIQQIAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEARGKAEAERMKLKAEAYQKYGDAAKMALVLDALPRIAAKIAAPLTKVDEIVVLSGDNSKVTSEVNRLLAELPASVHALTGVDLSKIPLIKKATGAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E614</accession>
    <name>A0A4W2E614_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sorting nexin 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SNX20</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027369417.1">
        <property type="nucleotide sequence ID" value="XM_027513616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E614"/>
    <dbReference type="SMR" id="A0A4W2E614"/>
    <dbReference type="STRING" id="30522.A0A4W2E614"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043864.1">
        <property type="protein sequence ID" value="ENSBIXP00000032198.1"/>
        <property type="gene ID" value="ENSBIXG00000014662.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017140.1">
        <property type="protein sequence ID" value="ENSBIXP00005009257.1"/>
        <property type="gene ID" value="ENSBIXG00005005704.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875288"/>
    <dbReference type="CTD" id="124460"/>
    <dbReference type="GeneTree" id="ENSGT00530000063759"/>
    <dbReference type="OMA" id="ISCQVRK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000025">
        <property type="entry name" value="Sorting nexin 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039937">
        <property type="entry name" value="SNX20/SNX21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20939">
        <property type="entry name" value="SORTING NEXIN 20, 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20939:SF1">
        <property type="entry name" value="SORTING NEXIN-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="74"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="316" mass="36172" checksum="3864DBA23ED24339" modified="2019-09-18" version="1">MASHKHPGSPGWTGPICQDMAGTTPKASAPRPDLPRPGPEDHLEAQGSPSSNSSMTTRELQEYWRAQKCCWKHVKLLFEIASARIEERKVSKFVMYQIVVIQTGSFDSNKAVLERRYSDFETLQKKLLKTFREEIEDVVFPKKHLIGNFTEEMISERKLALKEYLSVLYAIRCVRRSREFIDFLTRPELKEAFGCLRAGQYTKALDILMRVVPLQEKLTAHCPVLLVPALCAMLVCHRDLDRPAEAFAVGERALQCLQAREGHRYYAPLLDAMARLAYLLGKDFVSLQKRLEESQLRKPALRGFTLKELTVQEYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAK7</accession>
    <name>A0A4W2EAK7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement component C6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Together with component C5b, involved in MAC complex assembly: complement C5b and C6 associate with the outer leaflet of target cell membrane, reducing the energy for membrane bending.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAK7"/>
    <dbReference type="STRING" id="30522.A0A4W2EAK7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018868.1">
        <property type="protein sequence ID" value="ENSBIXP00000033019.1"/>
        <property type="gene ID" value="ENSBIXG00000002338.1"/>
    </dbReference>
    <dbReference type="OMA" id="YYRKNFC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048828">
        <property type="entry name" value="C6_KAZAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048831">
        <property type="entry name" value="C8A_B_C6_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003884">
        <property type="entry name" value="FacI_MAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF4">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21195">
        <property type="entry name" value="EGF_C8A_B_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21288">
        <property type="entry name" value="Kazal_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00057">
        <property type="entry name" value="FIMAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="14" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="19 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 26" id="KW-0732">Signal</keyword>
    <keyword evidence="10 25" id="KW-0768">Sushi</keyword>
    <keyword evidence="8" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="21" id="KW-1053">Target membrane</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021242576" description="Complement component C6" evidence="26">
        <location>
            <begin position="22"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="28">
        <location>
            <begin position="176"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="642"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="702"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="29">
        <location>
            <begin position="785"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="933" mass="104708" checksum="6E5A6CA871742D50" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAL6</accession>
    <name>A0A4W2EAL6_BOBOX</name>
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        <submittedName>
            <fullName evidence="6">Caspase recruitment domain family member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CARD9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAL6"/>
    <dbReference type="STRING" id="30522.A0A4W2EAL6"/>
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        <property type="protein sequence ID" value="ENSBIXP00000028822.1"/>
        <property type="gene ID" value="ENSBIXG00000010201.1"/>
    </dbReference>
    <dbReference type="OMA" id="HEVDWEN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050700">
        <property type="term" value="F:CARD domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000003">
        <property type="entry name" value="Caspase recruitment domain family, member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14559">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14559:SF3">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="474"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
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        <location>
            <begin position="364"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="486"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="538" mass="61754" checksum="A6F9F80D498426CC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAM6</accession>
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        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAM6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045191.1">
        <property type="protein sequence ID" value="ENSBIXP00000033938.1"/>
        <property type="gene ID" value="ENSBIXG00000016813.1"/>
    </dbReference>
    <dbReference type="OMA" id="KFYAMEG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:complement component C3b binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000060">
        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
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        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF7">
        <property type="entry name" value="COMPLEMENT FACTOR H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Complement control module/SCR domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
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        <property type="match status" value="4"/>
    </dbReference>
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    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
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        </location>
    </feature>
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        </location>
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        </location>
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            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033938.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="395" mass="45330" checksum="F12F8D49FBC46B96" modified="2019-09-18" version="1">MLMLINVLLTLWFYTAHGQGKHCNYPVIRHGQLYQSYSGYFPARVNQQFLYNCDQYFVPPSLYSSDYLTCTAEGWFPEEPCLRECIFNNLENGYTPSSEKKVVQGETVRVRCYHGYSLQNNQNTVTCTESGWSPPPRCIRVKNTGCSQPPQIDHGTINSSSSAEERREIHEQRLYAHGTKLSYTCEEGFKISENNVIICHMGKWSSPPQCVDVSGVNPPQVENAIIHNQKSRYQSGERARYECIGNYDLFGEIEIVCLNGTWTEPPQCKDSQGKCGPPPPIDNGDITSLLQSVYPPGSVVEYRCQAYYELRGNRNVVCRNGEWSEPPKCLEACVISEETMRKHHIQLRWKHDKKLYSKTEDTIEFMCQYGYRQLTPKHTFRATCREGKVVYPRCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAM7</accession>
    <name>A0A4W2EAM7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Myelin protein zero like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MPZL2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin P0 protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419410.1">
        <property type="nucleotide sequence ID" value="XM_027563609.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2EAM7"/>
    <dbReference type="STRING" id="30522.A0A4W2EAM7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009069.1">
        <property type="protein sequence ID" value="ENSBIXP00000028832.1"/>
        <property type="gene ID" value="ENSBIXG00000010226.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051929.1">
        <property type="protein sequence ID" value="ENSBIXP00005042765.1"/>
        <property type="gene ID" value="ENSBIXG00005026304.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905795"/>
    <dbReference type="CTD" id="10205"/>
    <dbReference type="GeneTree" id="ENSGT01030000234556"/>
    <dbReference type="OMA" id="FHEQPYP"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05880">
        <property type="entry name" value="IgV_EVA1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myelin protein zero-like 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029863">
        <property type="entry name" value="MPZL2_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000920">
        <property type="entry name" value="Myelin_P0-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869">
        <property type="entry name" value="MYELIN P0 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869:SF21">
        <property type="entry name" value="MYELIN PROTEIN ZERO-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00213">
        <property type="entry name" value="MYELINP0"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611036" evidence="11">
        <location>
            <begin position="27"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="215" mass="24456" checksum="CB0557BEAA3C678E" modified="2019-09-18" version="1">MYGKSPTRAVLFLLGLQLTALWPTAAVEIYTPRVLEAVNGTDVRLKCTFSSFAPVGDALTVTWNFRPRDGGPEQFVFYYHVDPFKPMSGRFKDRVAWDGNPERYDVSILLWKLQFDDNGTYTCQVKNPPDVDGLIGEIQLSVVQTVRFSEIHFLALAIGSACALMVIIVIVVVLFQHFRKKRRAERAHRVVEIKSKEEEKLNQEKKASVSLEYTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EC02</accession>
    <name>A0A4W2EC02_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Store-operated calcium entry-associated regulatory factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transmembrane protein 66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SARAF</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with STIM1; the interaction is inhibited by the interaction of STIM1 with EFHB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EC02"/>
    <dbReference type="SMR" id="A0A4W2EC02"/>
    <dbReference type="STRING" id="30522.A0A4W2EC02"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007481.1">
        <property type="protein sequence ID" value="ENSBIXP00000029341.1"/>
        <property type="gene ID" value="ENSBIXG00000010925.1"/>
    </dbReference>
    <dbReference type="OMA" id="WILKGSC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001256">
        <property type="term" value="P:regulation of store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009567">
        <property type="entry name" value="SARAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929:SF0">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06682">
        <property type="entry name" value="SARAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021367658" description="Store-operated calcium entry-associated regulatory factor" evidence="18">
        <location>
            <begin position="31"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="203"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="335" mass="36314" checksum="D6BBD81212862A0C" modified="2019-09-18" version="1">MAAAGGPAAAGRCALSSLLLLLLVVGTAQCWDERDRILLRDIKALTLYYDRYTTSRRLEPIPQLKCVGGTAGCDSYTPKVIQCQNRGWDGYDVQWECKTDLDVAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYQLDYTELGLKKLRESGKEHGFNSFSNYYSKLYSPESSSLGGVVTIVVLLAIAFGVYKFFLSDGHESPPPYSEDPPYSHRYQRFSSSAGPPPAGFKSEFTGPHGATAGFGSAFTGQQGHEHSGPGFWTGLGTGGILGYLFGSNRAATPFSDSWYPSYPPSYTSTWNSRAYSPLRGGPGSSSACSGSEPRTRTASGYGGTRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EED6</accession>
    <name>A0A4W2EED6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cyclin-dependent kinase inhibitor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CDK-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">p21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CDKN1A</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027380259.1">
        <property type="nucleotide sequence ID" value="XM_027524458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EED6"/>
    <dbReference type="SMR" id="A0A4W2EED6"/>
    <dbReference type="STRING" id="30522.A0A4W2EED6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004792.1">
        <property type="protein sequence ID" value="ENSBIXP00000030194.1"/>
        <property type="gene ID" value="ENSBIXG00000012058.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006841.1">
        <property type="protein sequence ID" value="ENSBIXP00005030769.1"/>
        <property type="gene ID" value="ENSBIXG00005010949.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881413"/>
    <dbReference type="CTD" id="1026"/>
    <dbReference type="GeneTree" id="ENSGT00940000159918"/>
    <dbReference type="OMA" id="NFAWERV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070557">
        <property type="term" value="C:PCNA-p21 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140677">
        <property type="term" value="F:molecular function activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140311">
        <property type="term" value="F:protein sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120283">
        <property type="term" value="F:protein serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071460">
        <property type="term" value="P:cellular response to cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071480">
        <property type="term" value="P:cellular response to gamma radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071493">
        <property type="term" value="P:cellular response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048144">
        <property type="term" value="P:fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:keratinocyte proliferation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:1905179">
        <property type="term" value="P:negative regulation of cardiac muscle tissue regeneration"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of cell growth"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of DNA biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of gene expression"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of vascular associated smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090402">
        <property type="term" value="P:oncogene-induced cell senescence"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048146">
        <property type="term" value="P:positive regulation of fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of programmed cell death"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000379">
        <property type="term" value="P:positive regulation of reactive oxygen species metabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090399">
        <property type="term" value="P:replicative senescence"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.365.10:FF:000003">
        <property type="entry name" value="cyclin-dependent kinase inhibitor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029841">
        <property type="entry name" value="CDKN1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778:SF1">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="18">
        <location>
            <begin position="20"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="80"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067398"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078037"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00080818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="161" mass="17971" checksum="BAC3B85D811301FC" modified="2019-09-18" version="1">MSELSRDAHQIPRSSKACRCLFGPVDSEQLRQDCDALMASCVQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPVGPRDDLGGGKRPSPSSALLQGTSQEDHLDLSLSCTLVTRSPERPEGTPGGPGPSQGRKRRQTSMTDFYHSKRRLICSKRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGT3</accession>
    <name>A0A4W2EGT3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGT3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047164.1">
        <property type="protein sequence ID" value="ENSBIXP00000036685.1"/>
        <property type="gene ID" value="ENSBIXG00000020164.1"/>
    </dbReference>
    <dbReference type="OMA" id="ITPTHIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF78">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 7A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="48"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="332" mass="37356" checksum="106A066B58EB08D9" modified="2019-09-18" version="1">EDKLFSEMKDELCYEHLNGSCVKTPYSPGPRLILYTVFIFGAVLAVFGNLLVMISILHFKQLHSPTNFLIASLACADFLVGVTVMPFSTVSSVESCWYFGQRYCQLHSCFEGSFCYASIYHLCFISLDRYITVTDPLVYPTRFTVSVSGMCIASSWLFSIIFSFSLFGTGANAAGLEDLVSALTCVGGCQIAVNQSWVLVNFLLFFIPTLVMIVLYSKIFLIAKQQARKIENSFQDRVAKRERKAARTLGVAVLAFLVSWLPYFLDSIIDAFLGFITPTYIYEILVWIAYYNSAMNPLIYAFFYPWFRKAIKLIVTGKVLRANSSTVNLFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHA5</accession>
    <name>A0A4W2EHA5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SLC35G solute transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EHA5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043598.1">
        <property type="protein sequence ID" value="ENSBIXP00000031806.1"/>
        <property type="gene ID" value="ENSBIXG00000014208.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049976.1">
        <property type="protein sequence ID" value="ENSBIXP00005040348.1"/>
        <property type="gene ID" value="ENSBIXG00005022983.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153249"/>
    <dbReference type="OMA" id="LPWHQRC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF32">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="40"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="191"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="338" mass="35207" checksum="192EF920EDDDE5EB" modified="2019-09-18" version="1">MAGSHPYFNLPDFTQPSPPSTPPSLSSHQRCRPSDATKGLLVALLGGGLPAGFVGPFSRMAYQASHFPSLELLICRCLFHLPIALLLKLRGDPLLGPPDVRGRACLHALLNVLSIGCAYSAVQVVPAGNAATVRKGSSTVCSALLALCLESQRLSGYDWCGLLGSTLGLIIIVGPGLGTLQEGTTGLYTALGYVLAFLGGLALSLGLLVYRSLDFPSCLPTVAFLFGLVGLVGSVPGLFMLQTPVLPKDPLSWSCVGAVGILALVSFVCVSYAVTKAHPALVCAVLHSEVVVALMLQYYVLYETVAPSDIMGAGVVLGSIAIITAQNLSCEREGQVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJ24</accession>
    <name>A0A4W2EJ24_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cell cycle checkpoint protein RAD17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RAD17</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation. Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites. May also serve as a sensor of DNA replication progression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Interacts with RAD1 and RAD9 within the 9-1-1 (RAD1-RAD9-HUS1) complex. Interacts with RAD9B, POLE, SNU13 and MCM7. DNA damage promotes interaction with ATR or ATM and disrupts interaction with the 9-1-1 (RAD1-RAD9-HUS1) complex. Interacts (when phosphorylated) with NBN; promoting recruitment of the MRN complex to DNA damage sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJ24"/>
    <dbReference type="SMR" id="A0A4W2EJ24"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018748.1">
        <property type="protein sequence ID" value="ENSBIXP00000032977.1"/>
        <property type="gene ID" value="ENSBIXG00000015676.1"/>
    </dbReference>
    <dbReference type="OMA" id="YNCLKMA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000714">
        <property type="entry name" value="cell cycle checkpoint protein RAD17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018324">
        <property type="entry name" value="Rad17/Rad24_fun/met"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00602">
        <property type="entry name" value="rad24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="129"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="615"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="666"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="682" mass="76758" checksum="CB5CFCED6335F7FE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJ76</accession>
    <name>A0A4W2EJ76_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Angiopoietin 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ANGPT4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027414729.1">
        <property type="nucleotide sequence ID" value="XM_027558928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJ76"/>
    <dbReference type="SMR" id="A0A4W2EJ76"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047600.1">
        <property type="protein sequence ID" value="ENSBIXP00000037293.1"/>
        <property type="gene ID" value="ENSBIXG00000020810.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035287.1">
        <property type="protein sequence ID" value="ENSBIXP00005041387.1"/>
        <property type="gene ID" value="ENSBIXG00005024442.1"/>
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        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
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        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
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    <keyword evidence="3" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
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        <location>
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            <end position="22"/>
        </location>
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    <feature type="chain" id="PRO_5044611132" evidence="10">
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            <end position="498"/>
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            <begin position="188"/>
            <end position="254"/>
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    <sequence length="498" mass="56030" checksum="8F46A3AF73F66F18" modified="2019-09-18" version="1">MPSPPAMLLGGLLLIVASTTVAQRRGQEAAGRRRAHRVQHGQCSYTFVLPEPEPCPPEPEAFGGSNSLQRDSPAATLNLGDWPSQRMRQLEKMLENNTQWLQKLERYIQMNLRLELAQAQQHMVQNQTATMLELGTSLLTQTTAQTRKLTDVEAQVLNQTSRMEIQLLETSLSTNKLEKQLLLQGHELHRLQGHNSALETRVQALETQQQAELASLRGEKERLRRLLGRQSGALAGLERTLRAASSNSSLLQRQQHQLLESVQRLVRVMAQGPASMRAADQLFQDCAEIQRFGANASGIYTIHVANVTEPRKVFCDMEASGGGWTLIQRRENGSVNFQRNWKDYKQGFGNPAGEHWLGNEVVHQLTSRATYSLRVELQDWEGNEAYAQYEHFQLGSEAQLYRLSLSGYSGSAGRQSSLVLQGTNFSTRDADNDNCLCKCAQMLSGGWWFDACGLSNLNGIYYPARHHVRKLNGIRWHYFQGPSYSLRTTRMMVRPSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERD1</accession>
    <name>A0A4W2ERD1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type phospholipid transporter</fullName>
            <ecNumber evidence="2">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ABCA1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="2"/>
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    <dbReference type="STRING" id="30522.A0A4W2ERD1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033910.1">
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000264">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC2_TM"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
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        <property type="entry name" value="ABCA"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF34">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="680"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1347"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1655"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1697"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1735"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1766"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1853"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="899"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1912"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1286"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1287"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2261" mass="254269" checksum="AD317347452706A1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESV8</accession>
    <name>A0A4W2ESV8_BOBOX</name>
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        <recommendedName>
            <fullName evidence="10">Immunoglobulin superfamily member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">IGSF3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESV8"/>
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        <property type="protein sequence ID" value="ENSBIXP00000040115.1"/>
        <property type="gene ID" value="ENSBIXG00000024161.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000191">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000604">
        <property type="entry name" value="immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000674">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000689">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000827">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000932">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000491">
        <property type="entry name" value="Immunoglobulin superfamily, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF21">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021400906" description="Immunoglobulin superfamily member 3" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1124"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="6"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="143"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="276"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="401"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="559"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="676"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="813"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="949"/>
            <end position="1097"/>
        </location>
    </feature>
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        <location>
            <begin position="997"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1194" mass="135399" checksum="4F8D742E7EECC861" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ET19</accession>
    <name>A0A4W2ET19_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Gephyrin</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="30">Molybdopterin molybdenumtransferase</fullName>
                <shortName evidence="30">MPT Mo-transferase</shortName>
                <ecNumber evidence="30">2.10.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="30">Domain E</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="30">Molybdopterin adenylyltransferase</fullName>
                <shortName evidence="30">MPT adenylyltransferase</shortName>
                <ecNumber evidence="30">2.7.7.75</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="30">Domain G</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="33" type="primary">GPHN</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Also has a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="function">
        <text evidence="30">Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="function">
        <text evidence="28">Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:35048"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>molybdopterin + ATP + H(+) = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="EC" id="2.7.7.75"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:31332"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 30">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 30">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Postsynaptic cell membrane</location>
            <topology evidence="29">Lipid-anchor</topology>
            <orientation evidence="29">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="30">Belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="30"/>
    <dbReference type="EC" id="2.7.7.75" evidence="30"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ET19"/>
    <dbReference type="SMR" id="A0A4W2ET19"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034871.1">
        <property type="protein sequence ID" value="ENSBIXP00000039935.1"/>
        <property type="gene ID" value="ENSBIXG00000006683.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099634">
        <property type="term" value="C:postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007529">
        <property type="term" value="P:establishment of synaptic specificity at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097112">
        <property type="term" value="P:gamma-aminobutyric acid receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072579">
        <property type="term" value="P:glycine receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MoCF_biosynth_CS"/>
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    </dbReference>
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        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-0966">Cell projection</keyword>
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    <keyword evidence="23" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15 30" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 30" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 30" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="18 30" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="11 30" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="18"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="MoaB/Mog" evidence="32">
        <location>
            <begin position="548"/>
            <end position="691"/>
        </location>
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        <location>
            <begin position="194"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="31">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00060421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="782" mass="84734" checksum="7B0ED358BD2160DC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUB0</accession>
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        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
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        <property type="protein sequence ID" value="ENSBIXP00005000841.1"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611194" description="peptidylprolyl isomerase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="13">
        <location>
            <begin position="53"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="189"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="222" mass="25535" checksum="C597CD8A215D6AAE" modified="2019-09-18" version="1">NPGIMHFLFRLILFFYVWGIFTAQGQKAEETTEEVKIEVLHRPENCSKTSKKGDLLNAHYDGFLAKDGSKFYCSRTQNEGHPKWFVLGVGQVIKGLDIGMMDMCPGEKRKLIIPPSFAYGKEGYAEGKIPPDATLIFEIELYAVTKGPRSVETFKQIDTDNDRQLSKTEISHYLKKEFEKDEKPRDQSYQTAVLEDFFKKNDHDGDGFISSKEYNVYQHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EW54</accession>
    <name>A0A4W2EW54_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EW54"/>
    <dbReference type="STRING" id="30522.A0A4W2EW54"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037920.1">
        <property type="protein sequence ID" value="ENSBIXP00000041268.1"/>
        <property type="gene ID" value="ENSBIXG00000025472.1"/>
    </dbReference>
    <dbReference type="OMA" id="PQMLPIF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042475">
        <property type="term" value="P:odontogenesis of dentin-containing tooth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070175">
        <property type="term" value="P:positive regulation of enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031501">
        <property type="entry name" value="Amelotin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36858">
        <property type="entry name" value="AMELOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36858:SF1">
        <property type="entry name" value="AMELOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15757">
        <property type="entry name" value="Amelotin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021468320" evidence="2">
        <location>
            <begin position="17"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="209" mass="21530" checksum="C23A81FCE986420D" modified="2019-09-18" version="1">MKAAILLFCLLGSTLSLPLNPALVLPPTKLVPDQATLLNPQQPNQVFSSLSLIPLTHMLTLGSNLQLLNPAAGMPSGTQTLPLALGGLKVQQQLQPQMLPVIVAHFGAQGTILSSEELQGTSQILTGLIFHPLFPGAILPTSQANPDAQNGILPAGQAGANPAAQGTPEDPFSTPSGTDDDFASTTPAGIQRGRPTTEETPTGSPKGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EWB8</accession>
    <name>A0A4W2EWB8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EWB8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037626.1">
        <property type="protein sequence ID" value="ENSBIXP00000041129.1"/>
        <property type="gene ID" value="ENSBIXG00000006320.1"/>
    </dbReference>
    <dbReference type="OMA" id="EDINSTW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="123"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="333" mass="37777" checksum="451D16EBFE97105C" modified="2019-09-18" version="1">MANQYSCLENPMNSMKRQKDRTLKDELPRSVGAQYATGDEWRNNSRKNEGMEPRQKQHPVVNVTGDRSNVRSCKEHYCIGTWNVRSMNQGKLGVVKQEMARVNINILGISKLRWTGMGEFNSDDHYINYCGPESLRRNGVSIIVNKRVQNAVLGCTLKNDRMISVHFQGKPFNVTVIQVCYPTSNAEEAEVEQFYEDLQDLLELTPQKDVLFVVGDWNAKGGSQETLGVTGKFGCGVQNEAGQRLIEFCQENALVVANTLFQQHKRRLYTWTSPDGQHQNQIDYILCSQRWRSSIQSAKTRPGADCGSDRELLIAKFRLKLKVGKPLDHSGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXD2</accession>
    <name>A0A4W2EXD2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gamma-aminobutyric acid receptor subunit alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">GABA(A) receptor subunit alpha-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">GABRA2</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Postsynaptic cell membrane</location>
            <topology evidence="25">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027400214.1">
        <property type="nucleotide sequence ID" value="XM_027544413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EXD2"/>
    <dbReference type="SMR" id="A0A4W2EXD2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038291.1">
        <property type="protein sequence ID" value="ENSBIXP00000041439.1"/>
        <property type="gene ID" value="ENSBIXG00000002072.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005603.1">
        <property type="protein sequence ID" value="ENSBIXP00005031163.1"/>
        <property type="gene ID" value="ENSBIXG00005011447.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894255"/>
    <dbReference type="CTD" id="2555"/>
    <dbReference type="GeneTree" id="ENSGT00940000157266"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19035">
        <property type="entry name" value="LGIC_ECD_GABAAR_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000002">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit alpha-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005432">
        <property type="entry name" value="GABBAa2_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047023">
        <property type="entry name" value="Gabra-2_ECD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
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    <sequence length="451" mass="51162" checksum="B8C114BEEDE089B3" modified="2019-09-18" version="1">MKTKLNSSNMQLLLFVFLAWDPARLVLANIQEDEAKNNITIFTRILDRLLDGYDNRLRPGLGDSITEVFTNIYVTSFGPVSDTDMEYTIDVFFRQKWKDERLKFKGPMNILRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRIQDDGTLLYTMRLTVQAECPMHLEDFPMDAHSCPLKFGSYAYTTSEVTYIWTYNASDSVQVAPDGSRLNQYDLLGQSIGKETIKSSTGEYTVMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKSVVNDKKKEKASVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKPENKPAEAKKTFNSVSKIDRMSRIVFPVLFGTFNLVYWATYLNREPVLGVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F192</accession>
    <name>A0A4W2F192_BOBOX</name>
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            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
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    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
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        <location>
            <begin position="42"/>
            <end position="66"/>
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        </location>
    </feature>
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            <begin position="58"/>
            <end position="303"/>
        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="350" mass="40008" checksum="E46BF942F3919C82" modified="2019-09-18" version="1">MAVEYNQSTDYYYEENEMNDTHDYSQYEVICIKEEVRKFAKVFLPAFFTIAFIIGLAGNSTVVAIYAYYKKRRTKTDVYILNLAVADLFLLFTLPFWAVNAVHGWVLGKIMCKVTSALYTVNFVSGMQFLACISTDRYWAVTKAPSQSGVGKPCWVICFCVWVAAILLSIPQLVFYTVNHKARCVPIFPYHLGTSMKASIQILEICIGFIIPFLIMAVCYFITAKTLIKMPNIKKSQPLKVLFTVVIVFIVTQLPYNIVKFCQAIDIIYSLITDCDMSKRMDVAIQITESIALFHSCLNPVLYVFMGTSFKNYIMKVAKKYGSWRRQRQNVEEIPFESEDATEPTSTFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2Y5</accession>
    <name>A0A4W2F2Y5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Nucleoporin Nup37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Nup107-160 subcomplex subunit Nup37</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">NUP37</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex includes NUP160, NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027397772.1">
        <property type="nucleotide sequence ID" value="XM_027541971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F2Y5"/>
    <dbReference type="SMR" id="A0A4W2F2Y5"/>
    <dbReference type="STRING" id="30522.A0A4W2F2Y5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052195.1">
        <property type="protein sequence ID" value="ENSBIXP00000043409.1"/>
        <property type="gene ID" value="ENSBIXG00000027519.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028494.1">
        <property type="protein sequence ID" value="ENSBIXP00005016928.1"/>
        <property type="gene ID" value="ENSBIXG00005003453.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892732"/>
    <dbReference type="CTD" id="79023"/>
    <dbReference type="GeneTree" id="ENSGT00390000010777"/>
    <dbReference type="OMA" id="FWKVQIK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000168">
        <property type="entry name" value="Nucleoporin Nup37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037626">
        <property type="entry name" value="NUP37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22806:SF0">
        <property type="entry name" value="NUCLEOPORIN NUP37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22806">
        <property type="entry name" value="NUCLEOPORIN NUP37 P37 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="14" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 22" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="22">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="326" mass="36863" checksum="B35542A7FE6C854C" modified="2019-09-18" version="1">MKQDATRNAAYTVDCEDYVHVVEFNPFESGDSGNLIAYGGNNFVFVGTCTFQEEEADVEGIQYKTLRTFHHGVRVDGIAWSPETRLDSLPPVIKFCTSAADMKIRLFTSDLQDKNEYKVLEGHSDFINGLVFDPREGQEIASVSDDHTCRIWNLEGIQTAHFVLHSPGMSVCWHPEETFKLMVAEKNGTIRFYDLLAQQAILSLESEQMPLMSAHWCLKNTFKVGAVAGNDWLIWDITRSSYPQDKRPVHMDRACLFRWSTVNENLFATTGYPGKMMSQFQIHHLGHPQPILIGSVAVGSGLSWHRTLPLCAIGSDHKLLFWVTEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3V9</accession>
    <name>A0A4W2F3V9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family with sequence similarity 151 member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAM151B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the menorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401520.1">
        <property type="nucleotide sequence ID" value="XM_027545719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3V9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052496.1">
        <property type="protein sequence ID" value="ENSBIXP00000043766.1"/>
        <property type="gene ID" value="ENSBIXG00000027920.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052606.1">
        <property type="protein sequence ID" value="ENSBIXP00005043571.1"/>
        <property type="gene ID" value="ENSBIXG00005004697.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895053"/>
    <dbReference type="CTD" id="167555"/>
    <dbReference type="GeneTree" id="ENSGT00530000063681"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019356">
        <property type="entry name" value="Menorin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184">
        <property type="entry name" value="MENORIN (DENDRITIC BRANCHING PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184:SF3">
        <property type="entry name" value="PROTEIN FAM151B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10223">
        <property type="entry name" value="Menorin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Menorin-like" evidence="2">
        <location>
            <begin position="29"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="276" mass="31395" checksum="27B45659302FFF5C" modified="2019-09-18" version="1">MTATAGGPGSWSENILEYFLRNNQITTEDGAQIVWYHAANHKVQVNEALRSTAHMIEADVILPSDGSEHGQPIMAHPPEINSDNTLQEWLAEVIKTNKGIKLDFKSLAAVEPSMMLLENVKRHLKRPVWINADVLPGPNGNSRVVDAKPFIDTVTSFSPDVTFSLGWTTGWHPEKVNEGYSWTMVKEMEYICNELNQPVTFPVRAALVRQSCSQLLWLLKQSNRYSLTIWTGKNDSYSTEDLLFIRDHFDKKRVFYDILEPQNHEFKQAIGIKINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3Y9</accession>
    <name>A0A4W2F3Y9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein NEDD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Neural precursor cell expressed developmentally down-regulated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NEDD1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with FAM29A. Interacts with HSPA1A and HSPA1B. Interacts with gamma-tubulin in a HSPA1A/B-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027397889.1">
        <property type="nucleotide sequence ID" value="XM_027542088.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397890.1">
        <property type="nucleotide sequence ID" value="XM_027542089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3Y9"/>
    <dbReference type="SMR" id="A0A4W2F3Y9"/>
    <dbReference type="STRING" id="30522.A0A4W2F3Y9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042757.1">
        <property type="protein sequence ID" value="ENSBIXP00000043799.1"/>
        <property type="gene ID" value="ENSBIXG00000005874.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023660.1">
        <property type="protein sequence ID" value="ENSBIXP00005036279.1"/>
        <property type="gene ID" value="ENSBIXG00005017834.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892774"/>
    <dbReference type="CTD" id="121441"/>
    <dbReference type="GeneTree" id="ENSGT00390000001561"/>
    <dbReference type="OMA" id="GTMVLWD"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000242">
        <property type="term" value="C:pericentriolar material"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:microtubule nucleation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:mitotic cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000284">
        <property type="entry name" value="protein NEDD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000302">
        <property type="entry name" value="protein NEDD1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052818">
        <property type="entry name" value="NEDD1_Spindle_Assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44414">
        <property type="entry name" value="PROTEIN NEDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44414:SF1">
        <property type="entry name" value="PROTEIN NEDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="383"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="508"/>
            <end position="522"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="659" mass="71204" checksum="3B5E69997BE5D158" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FFJ6</accession>
    <name>A0A4W2FFJ6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Chorionic somatomammotropin hormone 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC113881914</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the somatotropin/prolactin family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000001465.1"/>
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        <property type="gene ID" value="ENSBIXG00005000816.1"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:mammary gland development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of cell population proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903489">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0031667">
        <property type="term" value="P:response to nutrient levels"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001400">
        <property type="entry name" value="Somatotropin/Prolactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018116">
        <property type="entry name" value="Somatotropin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417:SF5">
        <property type="entry name" value="PROLACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417">
        <property type="entry name" value="SOMATOTROPIN,PROLACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00103">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00836">
        <property type="entry name" value="SOMATOTROPIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00266">
        <property type="entry name" value="SOMATOTROPIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00338">
        <property type="entry name" value="SOMATOTROPIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0372">Hormone</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611386" evidence="9">
        <location>
            <begin position="27"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601400-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="228" mass="26469" checksum="91C94B91E1785CA2" modified="2019-09-18" version="1">LLPHPSFYGSCLLLLLVVSNLVLCQGKSCLSCSPDVFVSLQKSLTDVFVYALSLSHDIHNLSTIMFNEFDEKYAQGKLYHINATKSCHTNSFHTPEEGDKAQQTNNEDLIKWTLVLLYCWNNPLYYLVPEIQSMKNLSEAVVSSAEEIENMSEKLESLIETHFGKIIFPVLKTIHEARIPWSRYSSMKFSNEDRRISEYYNLFYCLRRDSHKVDMYINILECRTRKTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FKX5</accession>
    <name>A0A4W2FKX5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CD24 molecule</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FKX5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026790.1">
        <property type="protein sequence ID" value="ENSBIXP00000036442.1"/>
        <property type="gene ID" value="ENSBIXG00000003577.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005184.1">
        <property type="protein sequence ID" value="ENSBIXP00005006019.1"/>
        <property type="gene ID" value="ENSBIXG00005011576.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018829"/>
    <dbReference type="OMA" id="TTKGHGN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030296">
        <property type="term" value="F:protein tyrosine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001775">
        <property type="term" value="P:cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028029">
        <property type="entry name" value="CD24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16676">
        <property type="entry name" value="SIGNAL TRANSDUCER CD24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16676:SF0">
        <property type="entry name" value="SIGNAL TRANSDUCER CD24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14984">
        <property type="entry name" value="CD24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611404" description="CD24 molecule" evidence="2">
        <location>
            <begin position="27"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8054" checksum="796A452352D14A07" modified="2019-09-18" version="1">MGKAIIARLGLGLLLLALLLPTQTYSNQTTVVTPSSNSSHTTSPASHPANATTKASDGTLQSTASLFVISVSLLHLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FXL9</accession>
    <name>A0A4W2FXL9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium two pore domain channel subfamily K member 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KCNK15</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027413952.1">
        <property type="nucleotide sequence ID" value="XM_027558151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FXL9"/>
    <dbReference type="STRING" id="30522.A0A4W2FXL9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024696.1">
        <property type="protein sequence ID" value="ENSBIXP00000035639.1"/>
        <property type="gene ID" value="ENSBIXG00000003297.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018369.1">
        <property type="protein sequence ID" value="ENSBIXP00005010126.1"/>
        <property type="gene ID" value="ENSBIXG00005014826.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902769"/>
    <dbReference type="CTD" id="60598"/>
    <dbReference type="GeneTree" id="ENSGT00940000160902"/>
    <dbReference type="OMA" id="ERKRWEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000057">
        <property type="entry name" value="Potassium channel subfamily K member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008073">
        <property type="entry name" value="TASK5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF18">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01690">
        <property type="entry name" value="TASK5CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611508" evidence="15">
        <location>
            <begin position="25"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="16">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="16">
        <location>
            <begin position="167"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="290"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="321" mass="35084" checksum="346B7CCCFC443EFF" modified="2019-09-18" version="1">MRKQSARTAALVLCILSYLLVGAAVFDALESEAESGRKRLLAQKRSELRRKYGFSAEDYRELERLALQAEPHRAGRQWKFAGSFYFAITVITTIGYGHAAPGTDSGKVFCMFYALLGIPLTLVTFQSLGERLNALVRRLLLAAKRCLGLRRPRVSPENMAVAGLLVCAGTLALGAAAFAHFEGWTFFHAYYYCFITLTTIGFGDFVALQNDEALQRKPPYVAFSFLYILLGLTVIGAFLNLVVLRFLAAGADAPERAARPLRRGAPENRGPARRAGLISVSCRLHRLELCSRDNPGFSPPASPGAAGGGRADRPPARRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GBN0</accession>
    <name>A0A4W2GBN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA repair nuclease/redox regulator APEX1</fullName>
            <shortName evidence="17">APEN</shortName>
            <shortName evidence="17">REF-1</shortName>
            <ecNumber evidence="17">3.1.11.2</ecNumber>
            <ecNumber evidence="17">3.1.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Redox factor-1</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="17">DNA repair nuclease/redox regulator APEX1, mitochondrial</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="20" type="primary">APEX1</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="15 17">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="17"/>
    <dbReference type="EC" id="3.1.21.-" evidence="17"/>
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        <property type="protein sequence ID" value="ENSBIXP00005015685.1"/>
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    <keyword evidence="7 17" id="KW-0227">DNA damage</keyword>
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    <keyword evidence="6 17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 15 17" id="KW-0479">Metal-binding</keyword>
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        <location>
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            <end position="309"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
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        <location>
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        <ligand>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000429181"/>
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    </evidence>
    <sequence length="318" mass="35584" checksum="40C733E00350738D" modified="2019-09-18" version="1">MPKRGKKGAVVEDAEEPKTEPEAKKSKAGAKKNEKEAVGEGAVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPVELQELSGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEYDAFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLTDSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSQSLLPALCDSKIRSKALGSDHCPITLYLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GLB6</accession>
    <name>A0A4W2GLB6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex I subunit B13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Complex I-13kD-B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">NADH-ubiquinone oxidoreductase 13 kDa-B subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NDUFA5</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027394916.1">
        <property type="nucleotide sequence ID" value="XM_027539115.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00000016256.1"/>
        <property type="gene ID" value="ENSBIXG00000003764.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031319.1">
        <property type="protein sequence ID" value="ENSBIXP00005018795.1"/>
        <property type="gene ID" value="ENSBIXG00005003748.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000008099"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
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            <dbReference type="ARBA" id="ARBA00004443"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016385"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030376"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032775"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="116" mass="13316" checksum="8AD6550D2ECD7B0D" modified="2019-09-18" version="1">MAGLLKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPDVKKLEERLQGGQIEEVILQAENELSLARKMIQWKPWEPLVEEPPASQWKWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GLG8</accession>
    <name>A0A4W2GLG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Troponin I, cardiac muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cardiac troponin I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TNNI3</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GLG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022586.1">
        <property type="protein sequence ID" value="ENSBIXP00000012594.1"/>
        <property type="gene ID" value="ENSBIXG00000017650.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048946.1">
        <property type="protein sequence ID" value="ENSBIXP00005017846.1"/>
        <property type="gene ID" value="ENSBIXG00005007761.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000002">
        <property type="entry name" value="troponin I, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF2">
        <property type="entry name" value="TROPONIN I, CARDIAC MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="184" mass="21206" checksum="416CF3DAF85FEE70" modified="2019-09-18" version="1">LILPCVLPRQKKSKISASRKLQLKTLMLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLNQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKETLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GMP3</accession>
    <name>A0A4W2GMP3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 21">5-hydroxytryptamine receptor 2A</fullName>
            <shortName evidence="21">5-HT-2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 21">Serotonin receptor 2A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">HTR2A</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">G-protein coupled receptor for 5-hydroxytryptamine (serotonin).</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts (via C-terminus) with MPDZ and PATJ. May interact (via C-terminus) with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Cell projection</location>
            <location evidence="1 21">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 21">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 21">Membrane</location>
            <location evidence="2 21">Caveola</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027413036.1">
        <property type="nucleotide sequence ID" value="XM_027557235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GMP3"/>
    <dbReference type="SMR" id="A0A4W2GMP3"/>
    <dbReference type="STRING" id="30522.A0A4W2GMP3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012775.1">
        <property type="protein sequence ID" value="ENSBIXP00000001999.1"/>
        <property type="gene ID" value="ENSBIXG00000008539.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030640.1">
        <property type="protein sequence ID" value="ENSBIXP00005018313.1"/>
        <property type="gene ID" value="ENSBIXG00005021735.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902220"/>
    <dbReference type="CTD" id="3356"/>
    <dbReference type="GeneTree" id="ENSGT01050000244937"/>
    <dbReference type="OMA" id="MVTIGIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098666">
        <property type="term" value="C:G protein-coupled serotonin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071886">
        <property type="term" value="F:1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001587">
        <property type="term" value="F:Gq/11-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030296">
        <property type="term" value="F:protein tyrosine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099589">
        <property type="term" value="F:serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045600">
        <property type="term" value="P:positive regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045821">
        <property type="term" value="P:positive regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044380">
        <property type="term" value="P:protein localization to cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15304">
        <property type="entry name" value="7tmA_5-HT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000455">
        <property type="entry name" value="5HT2A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF30">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00516">
        <property type="entry name" value="5HT2ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0085">Behavior</keyword>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17 21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 20" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0770">Synapse</keyword>
    <keyword evidence="15 20" id="KW-0807">Transducer</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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    <feature type="transmembrane region" description="Helical" evidence="21">
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        <location>
            <begin position="359"/>
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        <location>
            <begin position="91"/>
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            <begin position="424"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="470" mass="52571" checksum="85414E50CA9A91F0" modified="2019-09-18" version="1">MDILCEENTSLSSTTNSLMQLHADTRLYSTDFNSGEGNTSNAFNWTVDSENRTNLSCEGCLSPPCFSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSTLTILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADENFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEATLCVSDPGTRTKLASFSFLPQSSLSSEKLFQRSIHREPGSYGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNRDVIEALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKKPLQLILVNTIPALAYKSSQLQMGPKKNSKKDDKTTDNDCTMVALGKEHPEDAPADSSNTVNEKVSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GU29</accession>
    <name>A0A4W2GU29_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">PRELI domain containing 1</fullName>
        </submittedName>
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    </gene>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Pecora</taxon>
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    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2GU29"/>
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00950000182810"/>
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    <dbReference type="PANTHER" id="PTHR11158">
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="161"/>
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        <location>
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            <end position="193"/>
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    </feature>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="206" mass="23600" checksum="D11126E05D2C4410" modified="2019-09-18" version="1">MFRAEEPYRISGRTRPASKHVLTEDIVHREVTSDQKLLSRRLLTKTNRMPRWAERLFPANVAHSVYILEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYRVNSDNSGWTEIRREAWVSSSLFGVSRAVQEFGLARFKSNVTKTMKGFEYILAKLQGEAPPKTLVETAKEAKEKAKETALAATEKAKDLASKAATKKQQQQQQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GVS1</accession>
    <name>A0A4W2GVS1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">[histone H3]-lysine(9) N-methyltransferase</fullName>
            <ecNumber evidence="18">2.1.1.367</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">EHMT1</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60280"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="Rhea" id="RHEA-COMP:15546"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.367"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6)-methyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60284"/>
            <dbReference type="Rhea" id="RHEA-COMP:15541"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.367" evidence="18"/>
    <dbReference type="STRING" id="30522.A0A4W2GVS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011175.1">
        <property type="protein sequence ID" value="ENSBIXP00000028152.1"/>
        <property type="gene ID" value="ENSBIXG00000009255.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038139.1">
        <property type="protein sequence ID" value="ENSBIXP00005023359.1"/>
        <property type="gene ID" value="ENSBIXG00005026364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156002"/>
    <dbReference type="OMA" id="MPKSIMG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070742">
        <property type="term" value="F:C2H2 zinc finger domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046976">
        <property type="term" value="F:histone H3K27 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140948">
        <property type="term" value="F:histone H3K9 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140718">
        <property type="term" value="P:facultative heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20905">
        <property type="entry name" value="EHMT_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10535">
        <property type="entry name" value="SET_EHMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000005">
        <property type="entry name" value="Euchromatic histone-lysine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
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    <sequence length="1293" mass="139425" checksum="89566D07AE743CFE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <name>Mg(2+)</name>
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        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="154"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="271"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="416"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="576"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="332"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="582"/>
            <end position="590"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="891" mass="100499" checksum="7B234EB3386C90C8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HK55</accession>
    <name>A0A4W2HK55_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Zinc finger MYND domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZMYND10</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 15">Plays a role in axonemal structure organization and motility. Involved in axonemal pre-assembly of inner and outer dynein arms (IDA and ODA, respectively) for proper axoneme building for cilia motility. May act by indirectly regulating transcription of dynein proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts (via C-terminus) with DNAAF11 (via CS domain); this interaction stabilizes DNAAF11 at the protein level. Interacts (via C-terminus) with DNAL1; this interaction stabilizes DNAL1 at the protein level. Interacts with DNAAF4, HSPA8, IQUB, RUVBL2 and DYNTL5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ZMYND10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379108.1">
        <property type="nucleotide sequence ID" value="XM_027523307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HK55"/>
    <dbReference type="SMR" id="A0A4W2HK55"/>
    <dbReference type="STRING" id="30522.A0A4W2HK55"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007729.1">
        <property type="protein sequence ID" value="ENSBIXP00000029258.1"/>
        <property type="gene ID" value="ENSBIXG00000010822.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008622.1">
        <property type="protein sequence ID" value="ENSBIXP00005030190.1"/>
        <property type="gene ID" value="ENSBIXG00005010284.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880767"/>
    <dbReference type="CTD" id="51364"/>
    <dbReference type="GeneTree" id="ENSGT00940000153820"/>
    <dbReference type="OMA" id="LIHEAYC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905505">
        <property type="term" value="P:positive regulation of motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000009">
        <property type="entry name" value="Zinc finger MYND domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017333">
        <property type="entry name" value="UCP037948_Znf-MYND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052298">
        <property type="entry name" value="ZMYND10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244:SF7">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037948">
        <property type="entry name" value="UCP037948_Znf_MYND10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="393"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="439" mass="50336" checksum="9B3CDD81845DCFFC" modified="2019-09-18" version="1">MGDLELLLPGEADVLVRGLRSFQLRDMGSRGWNQQHENLEKLNMQAILDATASQGEPIQELLVTHGKIPTLVEELIAVEMWKQKVFPVLCKLEDFKPQNTFPIYMVLHHEASIINLLETVFFHKEVCESAEDTVLDLVDYCHRKLTLLVAQSGRGAPPEEESQYSSPMQELQKQAELMEFEIALKALSVLRYITDCVDSLSLSTLSRMLSTHNLPCLLVELLEHSPWSRREGGKLQQFESGRWQTVTPSEQQKLSKLDGQVWIALYNLLLSPEARARYCLTSFAKGQLLKLRAFLTDTLLDQLPNLADLQGFLAHLALTEAQPPKKDLVLEQIPEIWERLERENKGKWQAIAKHQLRHVFSPSEQDLRLQARRWAETYRLDVLEAVAPERPRCAYCSAEASKRCSRCQNEWYCCRECQVKHWEKHGKACVLAVQGDRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HNL1</accession>
    <name>A0A4W2HNL1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">GDP-fucose protein O-fucosyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">FUT11</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Protein O-fucosyltransferase that specifically catalyzes O-fucosylation of serine or threonine residues in EMI domains of target proteins, such as MMRN1, MMRN2 and EMID1. Attaches fucose through an O-glycosidic linkage. O-fucosylation of EMI domain-containing proteins may be required for facilitating protein folding and secretion. May also show alpha-(1,3)-fucosyltransferase activity toward the innermost N-acetyl glucosamine (GlcNAc) residue in biantennary N-glycan acceptors. However, this was tested with a library of synthetic substrates and this activity is unsure in vivo. May be involved in biosynthesis of Lewis X-carrying biantennary N-glycans that regulate neuron stem cell self-renewal during brain development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-seryl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:17914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:63645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-threonyl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70491"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:17915"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:70492"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Endoplasmic reticulum membrane</location>
            <topology evidence="1 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16 17">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="16"/>
    <dbReference type="RefSeq" id="XP_027386373.1">
        <property type="nucleotide sequence ID" value="XM_027530572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HNL1"/>
    <dbReference type="STRING" id="30522.A0A4W2HNL1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008195.1">
        <property type="protein sequence ID" value="ENSBIXP00000004266.1"/>
        <property type="gene ID" value="ENSBIXG00000010625.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018879.1">
        <property type="protein sequence ID" value="ENSBIXP00005034230.1"/>
        <property type="gene ID" value="ENSBIXG00005015094.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885318"/>
    <dbReference type="CTD" id="170384"/>
    <dbReference type="GeneTree" id="ENSGT00940000158983"/>
    <dbReference type="OMA" id="EHREWGV"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046922">
        <property type="term" value="F:peptide-O-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050714">
        <property type="term" value="P:positive regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017176">
        <property type="entry name" value="Alpha-1_3-FUT_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF198">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037332">
        <property type="entry name" value="Alpha1_3FUT_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 16 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611946" description="GDP-fucose protein O-fucosyltransferase" evidence="18">
        <location>
            <begin position="28"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="77"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="213"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR037332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="493" mass="55679" checksum="826A1301CA7212C5" modified="2019-09-18" version="1">MGAGRPGSVLAVLGVLGVCATRGPGLAAEGKTGGEAEWAEPWDGAVFRPPSALGAVGVARSPGAPRPGREEAVDLPVLLWWSPGLFPHFPGDSERIECARGACVASRDRRVRGDSRTRALLFYGTDFRASEAPLPRLAHQSWALLHEESPLNNFLLSHGPGIRLFNLTSTFSRYSDYPLPLQWLPGIAYLRRAAPPLEERAEWRRRGYAPLLYLQSHCDVPADRDRYVRELMRYIPVDSYGKCLQNRELPAPRLRDTATATTEDPELLAFLSRYKFHLALENAICDDYMTEKLWRPMHLGAVPVYRGSPSVRDWMPNNHSIILIDDFESPQKLAEFIDFLDKNDEEYMKYLAYKQPGGITNQFLLDSLKQREWGVNDPLLPNYLNGFECFVCDHELARLDAEKAHAASPGNIPVPEPYIAQPSHMDCPVPTPGFGSVEEIPENDSWKEMWLQDYWQGLDQGEALTAMIHNNETLQGKFWDYLTEIFMKRNQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HWH5</accession>
    <name>A0A4W2HWH5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Forkhead box protein K2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Interleukin enhancer-binding factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FOXK2</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of SIN3A-, but not SIN3B-, containing multiprotein complexes. Interacts with DVL1, DVL2 (when phosphorylated) and DVL3; the interaction induces DVL2 nuclear translocation. Interacts with SUDS3. Interacts with BAP1 (when phosphorylated); leading to recruit the PR-DUB complex and repress FOXK2 target genes. Accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex, at least composed of BAP1, one of ASXL1, ASXL2 or (probably) ASXL3 and one of MBD5 or MBD6. The PR-DUB core associates with a number of accessory proteins, including FOXK1, FOXK2, KDM1B, HCFC1 and OGT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027373581.1">
        <property type="nucleotide sequence ID" value="XM_027517780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HWH5"/>
    <dbReference type="STRING" id="30522.A0A4W2HWH5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017281.1">
        <property type="protein sequence ID" value="ENSBIXP00000032346.1"/>
        <property type="gene ID" value="ENSBIXG00000014889.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022336.1">
        <property type="protein sequence ID" value="ENSBIXP00005035740.1"/>
        <property type="gene ID" value="ENSBIXG00005016965.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877569"/>
    <dbReference type="CTD" id="3607"/>
    <dbReference type="GeneTree" id="ENSGT00940000155709"/>
    <dbReference type="OMA" id="AMKPVTY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20055">
        <property type="entry name" value="FH_FOXK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22723">
        <property type="entry name" value="FHA_FOXK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000030">
        <property type="entry name" value="Forkhead box protein K2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000025">
        <property type="entry name" value="Forkhead box protein K2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047397">
        <property type="entry name" value="FH_FOXK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047398">
        <property type="entry name" value="FHA_FOXK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF3">
        <property type="entry name" value="FORKHEAD BOX PROTEIN K2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="17">
        <location>
            <begin position="53"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="18">
        <location>
            <begin position="256"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="15">
        <location>
            <begin position="256"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="357"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="608"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="625"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="658" mass="68719" checksum="2429398FA1449E3C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HY84</accession>
    <name>A0A4W2HY84_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tissue kallikrein</fullName>
            <ecNumber evidence="8">3.4.21.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KLK9</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.35" evidence="8"/>
    <dbReference type="RefSeq" id="XP_027370796.1">
        <property type="nucleotide sequence ID" value="XM_027514995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HY84"/>
    <dbReference type="SMR" id="A0A4W2HY84"/>
    <dbReference type="STRING" id="30522.A0A4W2HY84"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045217.1">
        <property type="protein sequence ID" value="ENSBIXP00000011552.1"/>
        <property type="gene ID" value="ENSBIXG00000016789.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045698.1">
        <property type="protein sequence ID" value="ENSBIXP00005034870.1"/>
        <property type="gene ID" value="ENSBIXG00005015912.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876132"/>
    <dbReference type="CTD" id="284366"/>
    <dbReference type="GeneTree" id="ENSGT00940000162323"/>
    <dbReference type="OMA" id="LCRWAYP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000021">
        <property type="entry name" value="Kallikrein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000010">
        <property type="entry name" value="Kallikrein related peptidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF57">
        <property type="entry name" value="KALLIKREIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611992" description="tissue kallikrein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="23"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="251" mass="27788" checksum="7354D72B233D68B0" modified="2019-09-18" version="1">MRLGFLCALLSLLEGQGWADTRAIGAKECRPNSQPWQAGLFYLTHLFCGATLISDRWLLTAAHCRKRYLWVRLGEHHLWKWEGPEQLFRATDFFPHPGFNQDLTAQDHNNDIMLVRLPRKAHLGPAVQPLNLSQTCVSPGTQCLISGWGAVSSPKVEYPLTLQCANISILEPRLCRRAYPGHISDSMLCAGLWEGGRGSCQGDSGGPLVCNGTLAGVVSGGSEPCSRPRRPAVYTSVCHYLDWIQETMEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I5V3</accession>
    <name>A0A4W2I5V3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Reprimo, TP53 dependent G2 arrest mediator homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPRM</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the reprimo family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416272.1">
        <property type="nucleotide sequence ID" value="XM_027560471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I5V3"/>
    <dbReference type="STRING" id="30522.A0A4W2I5V3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039654.1">
        <property type="protein sequence ID" value="ENSBIXP00000023871.1"/>
        <property type="gene ID" value="ENSBIXG00000005440.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026681.1">
        <property type="protein sequence ID" value="ENSBIXP00005037592.1"/>
        <property type="gene ID" value="ENSBIXG00005019467.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903880"/>
    <dbReference type="CTD" id="56475"/>
    <dbReference type="GeneTree" id="ENSGT00390000010523"/>
    <dbReference type="OMA" id="LKCCNFS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043383">
        <property type="entry name" value="Reprimo_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28649:SF2">
        <property type="entry name" value="PROTEIN REPRIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28649">
        <property type="entry name" value="PROTEIN REPRIMO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="109" mass="11806" checksum="6B26B1BB0EB7AE73" modified="2019-09-18" version="1">MNPALGNQTDVAGLFLANSSEALERAMRCCTQASVVTDDGFAEGGPDERSLYIMRVVQIAVMCVLSLTVVFGIFFLGCNLLIKSEGMINFLVKDRRPSKEVEAVVVGPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I867</accession>
    <name>A0A4W2I867_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C-C motif chemokine receptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CCR3</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379350.1">
        <property type="nucleotide sequence ID" value="XM_027523549.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027379351.1">
        <property type="nucleotide sequence ID" value="XM_027523550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I867"/>
    <dbReference type="SMR" id="A0A4W2I867"/>
    <dbReference type="STRING" id="30522.A0A4W2I867"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010164.1">
        <property type="protein sequence ID" value="ENSBIXP00000028480.1"/>
        <property type="gene ID" value="ENSBIXG00000009695.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032300.1">
        <property type="protein sequence ID" value="ENSBIXP00005040104.1"/>
        <property type="gene ID" value="ENSBIXG00005022644.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880893"/>
    <dbReference type="CTD" id="1232"/>
    <dbReference type="GeneTree" id="ENSGT01020000230359"/>
    <dbReference type="OMA" id="DMGLLCE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15185">
        <property type="entry name" value="7tmA_CCR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000026">
        <property type="entry name" value="C-C chemokine receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002238">
        <property type="entry name" value="Chemokine_CCR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF649">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01108">
        <property type="entry name" value="CHEMOKINER3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="55"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="358" mass="41156" checksum="7DBD4D243B26DDCB" modified="2019-09-18" version="1">MATSADGIETVGEVAGTTPYDYEAALPCEKSNVKELAAQFLPPLYSLVFVTGLLGNVVVVVILTKYKRLRIMTNIYLLNLAISDVLFLFTLPFWIHYVRWNEWVFGHRMCKLLSGLYYMGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGIVTSIFTWVLAGLAALPEFFFHETQEEAGQTFCSPLYPEDNENAWKRFHALRMNILGLALPLLVMAICYSGIIKTLLRCPSKKKYKAIRLIFVIMVVFFIFWTPYNLVVLLFAFQMHLKADCEQSRQLDLAMLVTEVIAYTHCCVNPVIYAFVGERFRKHLRHFFHRYMATYLGKFMPFLPSEKLERTSSVSPSTGEQELSAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IDA4</accession>
    <name>A0A4W2IDA4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Copine-6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Copine VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CPNE6</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Binds phospholipid membranes in a calcium-dependent manner. Plays a role in dendrite formation by melanocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (via second C2 domain) with OS9 (via C-terminus); this interaction occurs in a calcium-dependent manner in vitro. May interact with NECAB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027409046.1">
        <property type="nucleotide sequence ID" value="XM_027553245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IDA4"/>
    <dbReference type="SMR" id="A0A4W2IDA4"/>
    <dbReference type="STRING" id="30522.A0A4W2IDA4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033707.1">
        <property type="protein sequence ID" value="ENSBIXP00000019885.1"/>
        <property type="gene ID" value="ENSBIXG00000004618.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052403.1">
        <property type="protein sequence ID" value="ENSBIXP00005043342.1"/>
        <property type="gene ID" value="ENSBIXG00005027008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899885"/>
    <dbReference type="CTD" id="9362"/>
    <dbReference type="GeneTree" id="ENSGT00940000161567"/>
    <dbReference type="OMA" id="YKTNRDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000063">
        <property type="entry name" value="Copine 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000126">
        <property type="entry name" value="Copine 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF5">
        <property type="entry name" value="COPINE-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="134"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00063842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="557" mass="61865" checksum="AF3015C2C3A78F79" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IEL6</accession>
    <name>A0A4W2IEL6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BolA-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BOLA2B</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins. Acts together with the monothiol glutaredoxin GLRX3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with GLRX3; forms a heterotrimeric complex composed by two BOLA2 molecules and one GLRX3 molecule; linked by [2Fe-2S] clusters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383778.1">
        <property type="nucleotide sequence ID" value="XM_027527977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IEL6"/>
    <dbReference type="SMR" id="A0A4W2IEL6"/>
    <dbReference type="STRING" id="30522.A0A4W2IEL6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012466.1">
        <property type="protein sequence ID" value="ENSBIXP00000002170.1"/>
        <property type="gene ID" value="ENSBIXG00000008656.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033539.1">
        <property type="protein sequence ID" value="ENSBIXP00005040645.1"/>
        <property type="gene ID" value="ENSBIXG00005023363.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883859"/>
    <dbReference type="CTD" id="654483"/>
    <dbReference type="GeneTree" id="ENSGT00510000047760"/>
    <dbReference type="OMA" id="VHAFSQK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000308">
        <property type="entry name" value="bolA-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045115">
        <property type="entry name" value="BOL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735:SF27">
        <property type="entry name" value="BOLA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735">
        <property type="entry name" value="BOLA-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="86" mass="10100" checksum="7BD2CBA6361E7992" modified="2019-09-18" version="1">MELSAEYLREKLQRDLEAEHVEVEDTTPNRCASSFRVLVVSAKFEGKPLLQRHRLVNTCLAEELLHIHAFEQKTLTPEQWAREQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IJ80</accession>
    <name>A0A4W2IJ80_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 22 member 23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC22A23</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="nucleotide sequence ID" value="XM_027525308.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000002019.1"/>
        <property type="gene ID" value="ENSBIXG00000008571.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005036981.1">
        <property type="protein sequence ID" value="ENSBIXP00005042149.1"/>
        <property type="gene ID" value="ENSBIXG00005004357.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157354"/>
    <dbReference type="OMA" id="QKNCISP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS_dom"/>
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    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
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    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="483"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="511"/>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        <location>
            <begin position="160"/>
            <end position="623"/>
        </location>
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            <begin position="1"/>
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        <location>
            <begin position="40"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042149.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="687" mass="73362" checksum="D13CA8EDDE7564A4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
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        <property type="term" value="P:lipid metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
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    </dbReference>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="328" mass="37917" checksum="1518FA3726F3BE1E" modified="2019-09-18" version="1">MPCFKQPKHFQSLVLLHWPLSYLGMFWILQPLAIYLLFTSWWPLPALYFVWFLLDWKTPEQGGRRSAWVRNWCVWTHIRDYFPISILKTKELSPEHNYLMGVHPHGLLAFGAFCNFCTEATGFLKIFPGITPHLATLSWFFKIPFIRDYLMAKGVCSVSQPAIDYLLSHGTGNLVGIVVGGVGEALQSVPNTTMLILQKRKGFVRMALQHGAHLVPTYTFGETEVYDQVLFHKDSRMYKFQSYFRQILGFYFCIFYGRGFRQGSTGLLPYPLPIVTVVGEPLPLPKIEKPSQEMVDKYHALYKKALTKLFDQHKTQYGCPETQKLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2INR4</accession>
    <name>A0A4W2INR4_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein WDR12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">WD repeat-containing protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">WDR12</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027420614.1">
        <property type="nucleotide sequence ID" value="XM_027564813.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2INR4"/>
    <dbReference type="STRING" id="30522.A0A4W2INR4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022078.1">
        <property type="protein sequence ID" value="ENSBIXP00000034358.1"/>
        <property type="gene ID" value="ENSBIXG00000017290.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005046975.1"/>
        <property type="gene ID" value="ENSBIXG00005031585.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906531"/>
    <dbReference type="CTD" id="55759"/>
    <dbReference type="GeneTree" id="ENSGT00930000150950"/>
    <dbReference type="OMA" id="DHKYVEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:ribonucleoprotein complex binding"/>
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        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="7"/>
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        <property type="entry name" value="GPROTEINBRPT"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="9">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="136"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
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            <begin position="185"/>
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    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="253"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="303"/>
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    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="338"/>
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        </location>
    </feature>
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            <begin position="221"/>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046975.1"/>
        </source>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="423" mass="47709" checksum="F321563DB8F91E00" modified="2019-09-18" version="1">MAQLQTRFFTDNKKYAVDDVPFSIPAASEIADLSNLINKLLEAKNEFHKHVEFDFLIKGQFLRMPLFKHMELENISSEEVVELEYVEKYTAPQPEQCMFHDDWISAIEGTEEWILTGSYDKTSRIWSLEGKSIMTIVGHTDVVKDVAWVKKDSLSCLLLSASMDQTILLWEWNVERNKVKALHCCRGHAGSVDSIAVDSTGTKFCSGSWDKMLKIWSTVPTDEEDEMEESTNRPRKKQKTEQLGLTRTPIVTLSGHKEAISSVLWSDAEEICSASWDHTIKVWDVESGSLKSTLTGNKVFNCISYSPLCKRLASGSTDRHIRLWDPRTKDGSLVSLSLTSHTGWVTSVKWSPTHEQQLISGSLDNMVKLWDTRSCKAPLYDLAAHEDKVLSVDWTDSGLLLSGGADNKLYSYRYSPTTSHVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMH1</accession>
    <name>A0A4W2BMH1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Presynapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMH1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053604.1">
        <property type="protein sequence ID" value="ENSBIXP00000000201.1"/>
        <property type="gene ID" value="ENSBIXG00000030111.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:regulation of synaptic plasticity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ELKS/RAB6-interacting/CAST family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019323">
        <property type="entry name" value="ELKS/CAST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861">
        <property type="entry name" value="ELKS/RAB6-INTERACTING/CAST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861:SF3">
        <property type="entry name" value="ERC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10174">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
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        <location>
            <begin position="932"/>
            <end position="967"/>
        </location>
    </feature>
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        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
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        </location>
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            <end position="953"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="967" mass="111733" checksum="BA1EC8A7D2B68BD9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BN30</accession>
    <name>A0A4W2BN30_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Beta-glucuronidase</fullName>
            <ecNumber evidence="5 13">3.2.1.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 13">Plays an important role in the degradation of dermatan and keratan sulfates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:17633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:83411"/>
            <dbReference type="EC" id="3.2.1.31"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Inhibited by L-aspartic acid.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.31" evidence="5 13"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BN30"/>
    <dbReference type="STRING" id="30522.A0A4W2BN30"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014197.1">
        <property type="protein sequence ID" value="ENSBIXP00000001366.1"/>
        <property type="gene ID" value="ENSBIXG00000028642.1"/>
    </dbReference>
    <dbReference type="OMA" id="IHDHVGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019391">
        <property type="term" value="P:glucuronoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000027">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000628">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000029">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007538">
        <property type="entry name" value="PRK10150.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066:SF67">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="17">
        <location>
            <begin position="40"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="15">
        <location>
            <begin position="226"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="16">
        <location>
            <begin position="329"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="652" mass="74458" checksum="84B0738CCA6E1A75" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSL3</accession>
    <name>A0A4W2BSL3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSL3"/>
    <dbReference type="STRING" id="30522.A0A4W2BSL3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012337.1">
        <property type="protein sequence ID" value="ENSBIXP00000002229.1"/>
        <property type="gene ID" value="ENSBIXG00000008713.1"/>
    </dbReference>
    <dbReference type="OMA" id="AFMQKSR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15452">
        <property type="entry name" value="7tmC_mGluR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000009">
        <property type="entry name" value="Glutamate receptor, metabotropic 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000176">
        <property type="entry name" value="metabotropic glutamate receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001786">
        <property type="entry name" value="GPCR_3_mGluR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01054">
        <property type="entry name" value="MTABOTROPC4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="446"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="642"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="684"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="446"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="770" mass="86281" checksum="A51533A1C66A03FA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSY8</accession>
    <name>A0A4W2BSY8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myomesin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Myomesin family member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MYOM1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
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        <source>
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        </source>
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    <sequence length="1657" mass="184839" checksum="6DCA8BD9885835F0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTG8</accession>
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        <recommendedName>
            <fullName evidence="3">Sodium channel protein type 10 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">The channel consists of an ion conducting pore forming alpha-subunit regulated by one or more associated auxiliary subunits SCN1B, SCN2B and SCN3B; electrophysiological properties may vary depending on the type of the associated beta subunits. Found in a number of complexes with PRX, DYNLT1 and PDZD2. Interacts with proteins such as FSTL1, PRX, DYNLT1, PDZD2, S100A10 and many others. Interacts with NEDD4 and NEDD4L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.</text>
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        <property type="term" value="C:voltage-gated sodium channel complex"/>
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        <property type="term" value="F:voltage-gated sodium channel activity involved in cardiac muscle cell action potential"/>
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        <property type="term" value="P:neuronal action potential"/>
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        <property type="term" value="P:regulation of atrial cardiac muscle cell membrane depolarization"/>
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        <property type="term" value="P:regulation of heart rate"/>
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        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 10 SUBUNIT ALPHA"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF24609"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002582.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="1903" mass="214009" checksum="438EE343A5319ED8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <location evidence="4">Sarcolemma</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386717.1">
        <property type="nucleotide sequence ID" value="XM_027530916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYE7"/>
    <dbReference type="SMR" id="A0A4W2BYE7"/>
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    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="857"/>
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        <location>
            <begin position="360"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    <evidence type="ECO:0000313" key="25">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1134" mass="123864" checksum="72356763E1915988" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C567</accession>
    <name>A0A4W2C567_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein-tyrosine sulfotransferase</fullName>
            <ecNumber evidence="14">2.8.2.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 14">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Can also form heterodimers with TPST1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C567"/>
    <dbReference type="SMR" id="A0A4W2C567"/>
    <dbReference type="STRING" id="30522.A0A4W2C567"/>
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        <property type="protein sequence ID" value="ENSBIXP00000007891.1"/>
        <property type="gene ID" value="ENSBIXG00000013777.1"/>
    </dbReference>
    <dbReference type="OMA" id="VMMDSNH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000290">
        <property type="entry name" value="Protein-tyrosine sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021503433" description="Protein-tyrosine sulfotransferase" evidence="15">
        <location>
            <begin position="22"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
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        </source>
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        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007891.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="377" mass="41967" checksum="9DC99F2A9D4288D8" modified="2019-09-18" version="1">MRLSMRRALLAAGLALALVLAVHLGQRVLECQAVLGGPRGPRRTMRPEQEDLMMVGADHVEYRYGKAMPLIFVGGVPRSGTTLMRAMLDAHPEVRCGEETRIIPRVLAMRQAWSKSGREKLRLDEAGVTDEVLDAAMQAFILEVIAKHGEPARVLCNKDPFTLKSSVYLSRLFPNSKFLLMVRDGRASVHSMITRKVTIAGFDLSSYRDCLTKWNKAIEVMYAQCMEVGRDKCLPVYYEQLVLHPRRSLKVILDFLGIAWSDAVLHHEDLIGKPGGVSLSKIERSTDQVIKPVNLEALSKWTGHIPGDVLRDMAQIAPMLARLGYDPYANPPNYGNPDPIVINNTHRVLKGDYKTPANLKGYFQVNLNSTSSHLGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5U8</accession>
    <name>A0A4W2C5U8_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="ENSBIXG00005015497.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="69" mass="7694" checksum="1E305D22873B6FD5" modified="2019-09-18" version="1">MSATSVDQRPKGQGNKVSVQNGSIHQKDAVNDDDFEPYLSTQTNQGLRRLVFWHSTEMLQVCLGETWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8C2</accession>
    <name>A0A4W2C8C2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSBIXT00000011708.1">
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        <property type="gene ID" value="ENSBIXG00000000214.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0030130">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EH_dom"/>
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    </dbReference>
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        <property type="entry name" value="SYNRG_C"/>
    </dbReference>
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        <property type="entry name" value="AP1 GAMMA SUBUNIT BINDING PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="SYNERGIN GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
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            <end position="406"/>
        </location>
    </feature>
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        <location>
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            <end position="200"/>
        </location>
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        </location>
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        </location>
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        </location>
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        <location>
            <begin position="462"/>
            <end position="496"/>
        </location>
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        <location>
            <begin position="597"/>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="761"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1314" mass="139909" checksum="E656A9244F2F64C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8D1</accession>
    <name>A0A4W2C8D1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">KSR2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027422516.1">
        <property type="nucleotide sequence ID" value="XM_027566715.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2C8D1"/>
    <dbReference type="STRING" id="30522.A0A4W2C8D1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017165.1">
        <property type="protein sequence ID" value="ENSBIXP00000009107.1"/>
        <property type="gene ID" value="ENSBIXG00000001840.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034754.1">
        <property type="protein sequence ID" value="ENSBIXP00005041188.1"/>
        <property type="gene ID" value="ENSBIXG00005004124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907493"/>
    <dbReference type="CTD" id="283455"/>
    <dbReference type="GeneTree" id="ENSGT00940000158519"/>
    <dbReference type="OMA" id="DSWDRPH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C1_KSR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14153">
        <property type="entry name" value="PK_KSR2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="C1-like_sf"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="KSR_SAM-like_dom"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="Ser_Thr_protein_kinase"/>
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        <property type="entry name" value="KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_KSR1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_DAG_PE_2"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
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    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="412"/>
            <end position="456"/>
        </location>
    </feature>
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        <location>
            <begin position="666"/>
            <end position="931"/>
        </location>
    </feature>
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        <location>
            <begin position="239"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="498"/>
            <end position="554"/>
        </location>
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            <begin position="259"/>
            <end position="273"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
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        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="950" mass="107588" checksum="6F66F0278D3B14CF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9S5</accession>
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        <submittedName>
            <fullName evidence="4">BICD family like cargo adaptor 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">BICDL1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_027566595.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000008610.1"/>
        <property type="gene ID" value="ENSBIXG00000014399.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="P:Golgi to secretory granule transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
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    </dbReference>
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        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 1"/>
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    </dbReference>
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    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
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        <location>
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        </location>
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        <location>
            <begin position="124"/>
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        </location>
    </feature>
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            <end position="377"/>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
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        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="576" mass="65136" checksum="B794A76E6E075A83" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA30</accession>
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        <recommendedName>
            <fullName evidence="3">Centrosomal protein of 76 kDa</fullName>
        </recommendedName>
    </protein>
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        <name evidence="12" type="primary">CEP76</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with CCP110 and CEP97.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
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            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CEP76 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381412.1">
        <property type="nucleotide sequence ID" value="XM_027525611.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2CA30"/>
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        <property type="protein sequence ID" value="ENSBIXP00000003153.1"/>
        <property type="gene ID" value="ENSBIXG00000009546.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009292.1">
        <property type="protein sequence ID" value="ENSBIXP00005003960.1"/>
        <property type="gene ID" value="ENSBIXG00005000742.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000000781"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046599">
        <property type="term" value="P:regulation of centriole replication"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.30:FF:000003">
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CEP76"/>
    </dbReference>
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        <property type="entry name" value="CEP76-C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056288">
        <property type="entry name" value="CEP76_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056289">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="32"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="362" mass="41141" checksum="ACAD1E27653A78A3" modified="2019-09-18" version="1">MNKASAGVGIPVELFQILKDDAVKVLHSICQKIWKTQQWPQDWKRAVFIPIPKKDNAKECSNCYTIALISHASKVMLKILQARLQQHVNRKLPDVQAGFRKGRGTRDQIANICWIIKKAREFQKNIFFCFIDYSKAFDCVDHKKLWKILKEMGIPDHLTCLLRNLYAGQEATVRSGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIIRNPGLEEAQAGIKIARRHINNLRYANDTTLMAESEEELKSLLMKVKEESEKVGLKLNIWKTKIMASGPITSWEVDGETVETVADLGAPKSLQMVTAAMQLKDTYLLGRKVMTNLDSILKSRDITLPTKVHLVKAMVFPVVMYGCESWTIMKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CG20</accession>
    <name>A0A4W2CG20_BOBOX</name>
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        <submittedName>
            <fullName evidence="12">E74 like ETS transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ELF2</name>
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    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027423174.1">
        <property type="nucleotide sequence ID" value="XM_027567373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CG20"/>
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        <property type="protein sequence ID" value="ENSBIXP00000005251.1"/>
        <property type="gene ID" value="ENSBIXG00000011491.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009647.1">
        <property type="protein sequence ID" value="ENSBIXP00005003779.1"/>
        <property type="gene ID" value="ENSBIXG00005009855.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907772"/>
    <dbReference type="CTD" id="1998"/>
    <dbReference type="GeneTree" id="ENSGT00940000154953"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000066">
        <property type="entry name" value="ETS-related transcription factor Elf-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022084">
        <property type="entry name" value="TF_Elf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF10">
        <property type="entry name" value="ETS-RELATED TRANSCRIPTION FACTOR ELF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12310">
        <property type="entry name" value="Elf-1_N"/>
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    </dbReference>
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        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
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        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
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    </dbReference>
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        <property type="entry name" value="ETS_DOMAIN_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0010">Activator</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="208"/>
            <end position="290"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
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            <begin position="146"/>
            <end position="201"/>
        </location>
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        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
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            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
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            <begin position="182"/>
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        </location>
    </feature>
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            <begin position="370"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="594" mass="63876" checksum="FD6E6856FC8F6B17" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGF4</accession>
    <name>A0A4W2CGF4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGF4"/>
    <dbReference type="STRING" id="30522.A0A4W2CGF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021803.1">
        <property type="protein sequence ID" value="ENSBIXP00000012090.1"/>
        <property type="gene ID" value="ENSBIXG00000017204.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
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        <property type="entry name" value="PNRC1/2"/>
    </dbReference>
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        <property type="entry name" value="PROLINE-RICH NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <location>
            <begin position="93"/>
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        </location>
    </feature>
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        <location>
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            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="137"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="328" mass="35671" checksum="FE09B1827E7EDF1E" modified="2019-09-18" version="1">MTVVSVPQREPLVLGGRLASMGFSTRGYFGALPMVTTAPPPLPRIPDPRALPPTLFLPHFLGGDGPCLTPQPRAPAPLPNCSLAAAGGTPRAAPKKRRKKKVRTSPSGQLPSRFHQYQQHRPSLEGGRSPATGLGGAQQVPDQAQAAAWVPAPTTATRTEEASPLLGPLPPAAPGQPSFRREVLKSKMGKSEKIAIPHGQLVHGIPLCEQPKINRQKNKYNLPLTKITSAKRNENNFWQDSVSSDIIQKQEKPFKNTENIKNKHLKKSSFLTEVSQKENYAGAKFSDPPSPSVLPKPPSHWMGTTIENSNQNRELMAVHLKTLLKVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CH61</accession>
    <name>A0A4W2CH61_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CH61"/>
    <dbReference type="STRING" id="30522.A0A4W2CH61"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022332.1">
        <property type="protein sequence ID" value="ENSBIXP00000012420.1"/>
        <property type="gene ID" value="ENSBIXG00000017503.1"/>
    </dbReference>
    <dbReference type="OMA" id="DPMSEGK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029200">
        <property type="entry name" value="TMEM247"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36691">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36691:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15444">
        <property type="entry name" value="TMEM247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="207" mass="23482" checksum="FBBBEFBD970E6229" modified="2019-09-18" version="1">MATEDREMMEGRGAGESCPTFPKMAPDDPMSEGKPRASLEADTPKPDSSYDYLEEMETCEDGGCPGPPKVLSSKAGPATTKGQAGDGLELAELPSVPATERDAEMELEKVRMEFELKRLKYLHEENERQRQHEEVMEQLQQQATPRLFSGGLQDLLLPQNQFAMFLYCFIFIHIIYVTKEMIFFLFSKHYLFCIAAILLCLIKTLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIK0</accession>
    <name>A0A4W2CIK0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dystrotelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DYTN</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIK0"/>
    <dbReference type="STRING" id="30522.A0A4W2CIK0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045822.1">
        <property type="protein sequence ID" value="ENSBIXP00000012931.1"/>
        <property type="gene ID" value="ENSBIXG00000017943.1"/>
    </dbReference>
    <dbReference type="OMA" id="FLSWVQS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16243">
        <property type="entry name" value="EFh_DYTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF18">
        <property type="entry name" value="DYSTROTELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="223"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="583" mass="65152" checksum="C1C185FA108A650B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLS2</accession>
    <name>A0A4W2CLS2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLS2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024787.1">
        <property type="protein sequence ID" value="ENSBIXP00000014068.1"/>
        <property type="gene ID" value="ENSBIXG00000006676.1"/>
    </dbReference>
    <dbReference type="OMA" id="ILVWKES"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="377" mass="43515" checksum="8B3008B9F47D6C01" modified="2019-09-18" version="1">MLSGNVATFHFILVWKESLARCWFQHQNLGPQILQARFQQYVNRELPDGQAGFRKGRGTRDQIANICWIMEKAREFQKSIYFCFIDHAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGRVRQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNLNNLRYADDTTLMAQSEEELKSLLMNVKEESEKVGFKLNIQKTKIMASGPITSWQIDGERVETVSDFIFGGSKITSDGDCSHEIKRRLLLGRKVMTNLDSILKSRDITLPTKVRLVKAMVFPVVMYGYESWTVKKAEHRKIDAFELWCWGRLLRVPWTARRSNQSILKEISPGCSLEGKMLKLKLQYFGHLMTHWKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMR9</accession>
    <name>A0A4W2CMR9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PRAME family member 9/15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRAME family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMR9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023578.1">
        <property type="protein sequence ID" value="ENSBIXP00000013263.1"/>
        <property type="gene ID" value="ENSBIXG00000018215.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000023599.1">
        <property type="protein sequence ID" value="ENSBIXP00000013266.1"/>
        <property type="gene ID" value="ENSBIXG00000006109.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000045965.1">
        <property type="protein sequence ID" value="ENSBIXP00000013259.1"/>
        <property type="gene ID" value="ENSBIXG00000018219.1"/>
    </dbReference>
    <dbReference type="OMA" id="NCNWILP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000435">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
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        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53660" checksum="36BF3DDC87BE5C61" modified="2019-09-18" version="1">MSAPTPPRLLDLAGRHLLRAEDLDVSTLESVPTELFPPLFLEAFDGYRTETLKAMVQTWPFVRLPLGALIDLPHVGPLQAVLEALDVLLAQKVRSRRCKLRVLDLRKTGQNFWSMWSGASSYGCSGSRTAAVAEPRSMTRQPSTPLKVFLDLCLKKRTLDNFLTYFLRWVEQRKSSIHLCCKKLKIVSMPMDKIVKVLSTVQLDCIQEVQVSCTWSLSTLATLAPFLGEMSNLQRLHLSHVHVSAFKKQEHDHVVQITSQFRRLGHLRDLHLESPSFLEGCLDQMLRCLRSPLDSLSITKCRLTESDLTHVSQSPDISQLKSLDLSGVPMTDFRPELLQVLLEKVAATLQEVDLDACGITDSQLEAFLPALSRCSQLRAVSLCGNLLSTAVLEKLLRHTAVLPCLRQERYPAPQESYRPRGVLLEARLARLRAQLLEILRDLGRPRIIWISLSPCPRCGEDVCHHMEPIVYSCPAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRR2</accession>
    <name>A0A4W2CRR2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein ZFPM2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Friend of GATA protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Zinc finger protein multitype 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Transcription regulator that plays a central role in heart morphogenesis and development of coronary vessels from epicardium, by regulating genes that are essential during cardiogenesis. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA4, GATA5 and GATA6. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. Also required in gonadal differentiation, possibly be regulating expression of SRY. Probably acts a corepressor of NR2F2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRR2"/>
    <dbReference type="STRING" id="30522.A0A4W2CRR2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046561.1">
        <property type="protein sequence ID" value="ENSBIXP00000014546.1"/>
        <property type="gene ID" value="ENSBIXG00000019200.1"/>
    </dbReference>
    <dbReference type="OMA" id="VEFFCNK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055008">
        <property type="term" value="P:cardiac muscle tissue morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003151">
        <property type="term" value="P:outflow tract morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19216">
        <property type="entry name" value="PR-SET_ZFPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000828">
        <property type="entry name" value="Zinc finger protein, FOG family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001371">
        <property type="entry name" value="Zinc finger protein, FOG family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059121">
        <property type="entry name" value="CCHC_ZFPM2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039746">
        <property type="entry name" value="FOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049361">
        <property type="entry name" value="ZFPM1/2_PR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034731">
        <property type="entry name" value="Znf_CCHC_FOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958">
        <property type="entry name" value="FRIEND OF GATA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN ZFPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25445">
        <property type="entry name" value="CCHC_ZFPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21182">
        <property type="entry name" value="FOG1-like_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51810">
        <property type="entry name" value="ZF_CCHC_FOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC FOG-type" evidence="22">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC FOG-type" evidence="22">
        <location>
            <begin position="542"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC FOG-type" evidence="22">
        <location>
            <begin position="681"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC FOG-type" evidence="22">
        <location>
            <begin position="848"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC FOG-type" evidence="22">
        <location>
            <begin position="1113"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="389"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="637"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1006"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1051"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="447"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1006"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1057"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1077"/>
            <end position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1151" mass="127904" checksum="47FC156292C379BF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTD6</accession>
    <name>A0A4W2CTD6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Membrane-type matrix metalloproteinase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Membrane-type-1 matrix metalloproteinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">MMP15</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="21">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTD6"/>
    <dbReference type="STRING" id="30522.A0A4W2CTD6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044206.1">
        <property type="protein sequence ID" value="ENSBIXP00000009549.1"/>
        <property type="gene ID" value="ENSBIXG00000007071.1"/>
    </dbReference>
    <dbReference type="OMA" id="GCQEHVD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF25">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 26" id="KW-0732">Signal</keyword>
    <keyword evidence="5 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021271736" description="Membrane-type matrix metalloproteinase 1" evidence="26">
        <location>
            <begin position="46"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="27">
        <location>
            <begin position="135"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="23">
        <location>
            <begin position="367"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="23">
        <location>
            <begin position="416"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="23">
        <location>
            <begin position="463"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="23">
        <location>
            <begin position="512"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="300"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="564"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="599"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="21">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="22">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="675" mass="76239" checksum="DDA28947909AA798" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CU70</accession>
    <name>A0A4W2CU70_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">SLC1A1</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>K(+)(in) + L-cysteine(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-cysteine(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:82559"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Membrane</location>
            <topology evidence="28">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Recycling endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CU70"/>
    <dbReference type="STRING" id="30522.A0A4W2CU70"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029067.1">
        <property type="protein sequence ID" value="ENSBIXP00000016892.1"/>
        <property type="gene ID" value="ENSBIXG00000020977.1"/>
    </dbReference>
    <dbReference type="OMA" id="ICSFVVP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033229">
        <property type="term" value="F:cysteine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070778">
        <property type="term" value="P:L-aspartate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070633">
        <property type="term" value="P:transepithelial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150104">
        <property type="term" value="P:transport across blood-brain barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3860.10:FF:000002">
        <property type="entry name" value="Amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF109">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 28" id="KW-0769">Symport</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="9 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 28" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="524" mass="57209" checksum="AA5365F8D9B56BC4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUC2</accession>
    <name>A0A4W2CUC2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-D1-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CCNDBP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts with CCND1 and GRAP2. May also interact with COPS5, RPLP0, SIRT6, SYF2 and TCF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCNDBP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUC2"/>
    <dbReference type="SMR" id="A0A4W2CUC2"/>
    <dbReference type="STRING" id="30522.A0A4W2CUC2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047739.1">
        <property type="protein sequence ID" value="ENSBIXP00000016956.1"/>
        <property type="gene ID" value="ENSBIXG00000021036.1"/>
    </dbReference>
    <dbReference type="OMA" id="HEATKFC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000005">
        <property type="entry name" value="cyclin-D1-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000008">
        <property type="entry name" value="Cyclin-D1-binding protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049318">
        <property type="entry name" value="GCIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GCIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GCIP_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="9">
        <location>
            <begin position="56"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like C-terminal" evidence="10">
        <location>
            <begin position="218"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="358" mass="39664" checksum="D74A188ADE659D01" modified="2019-09-18" version="1">MESAAVSAAPDATLDPPLEQLRHLAGELRQLLPGVRVGEAQETTKEFDREAFWRRFNEAAVTVSREATTLTVAFSRLPLPSPQETQRFCEQVHAAIKAIIGVYYLFPKDQGITLRKLVRSATLDIVDDMAQLVEALYINPAQSSPENLISYNSVWDACQHVPQIPKDNKAAALSVLTKSVDLVKDAHEEMEQAVEECDPYCGLLNDIEEDSSDNHVDEDILGCPNNRDSYWSEEDQELIIPCLALARASKACLKKIRLSVAENGKKDQVAQLDDIVDISDEISPSVDDLALSIYPPVCPLTVRINSAKLASVLKKALEITKASHVTPQPEDSWIPLLINAVDHCMNRIKELTQNEVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUI8</accession>
    <name>A0A4W2CUI8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Testis expressed 29</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2CUI8"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37339:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 29"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24155" checksum="5F8205E154CB6C3E" modified="2019-09-18" version="1">MRYAPEFKKSPSHLLKKFAVCDIPLYDICDYNVSRDRCKELGCCFYKGICYEKAVPSYVQVFSALIVIIAGAFVITIIYRSALSFSEPHLRARGDRNRVLCGRAAPRLAAVWATRRAVFAHEPARVRAFLPSTLRADASGTEGRWCQPGSSHLSLTASERGLQAARPHPRPALLRGRLVLRHASGVRVSGQDPPSRPRSCHRSSGRAGLQPALGPPAPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYF1</accession>
    <name>A0A4W2CYF1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYF1"/>
    <dbReference type="STRING" id="30522.A0A4W2CYF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045071.1">
        <property type="protein sequence ID" value="ENSBIXP00000011375.1"/>
        <property type="gene ID" value="ENSBIXG00000016625.1"/>
    </dbReference>
    <dbReference type="OMA" id="SCCGCCQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="169" mass="18019" checksum="5F827AB878143D63" modified="2025-10-08" version="2">MTHFTFMEIYKSITPCCRPVRCQTTCCRTTCLKPICVTTCCQPTCCESSCCQPSCPQTCCQITETTCCKPTCVTSCCQPTCCASSSAGQTCGGSNCCQPCCQPASCAPVYCHRTCYHPTCLCLPGCQDQSCGSSCCQPCSRPVCCQTTCCRTTCCRPSCVSSCCQPSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D004</accession>
    <name>A0A4W2D004_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Amphoterin-induced protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">AMIGO-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Binds itself as well as AMIGO1 and AMIGO3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily. AMIGO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D004"/>
    <dbReference type="STRING" id="30522.A0A4W2D004"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048010.1">
        <property type="protein sequence ID" value="ENSBIXP00000017496.1"/>
        <property type="gene ID" value="ENSBIXG00000021495.1"/>
    </dbReference>
    <dbReference type="OMA" id="DFVWVGP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001047">
        <property type="entry name" value="amphoterin-induced protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000170">
        <property type="entry name" value="amphoterin-induced protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031283">
        <property type="entry name" value="AMIGO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24368">
        <property type="entry name" value="AMPHOTERIN-INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24368:SF209">
        <property type="entry name" value="AMPHOTERIN-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="372"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="591" mass="66180" checksum="EF5B5C0A117D6347" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0J0</accession>
    <name>A0A4W2D0J0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="4">Centromere protein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CENPO</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2D0J0"/>
    <dbReference type="STRING" id="30522.A0A4W2D0J0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030412.1">
        <property type="protein sequence ID" value="ENSBIXP00000017690.1"/>
        <property type="gene ID" value="ENSBIXG00000021648.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23836">
        <property type="entry name" value="DRWD-C_CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23835">
        <property type="entry name" value="DRWD-N_CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT MAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
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        <location>
            <begin position="41"/>
            <end position="68"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="296" mass="33858" checksum="32F300CF279D33A0" modified="2019-09-18" version="1">MELANTLRQDGESRGGVLAHLERLETQVSKSHKKLEEPQNAQALEARIHELKRLRDKLRAEVKQRQARVKASTANVEPDQILEISEQEILERKQENMKAILQAYRFTGISGKLTSRGVSVCISTAFEGNLLDSYFVDLVMEKPLWIHHHSVPVFIPLEEISAKYLQTNTRHFLFVLWEYLNAYSGRKYQADRLQSDFAAFLVGPLQRNSLCNLLSFTYKVKPEGQSFPFCARLLYKDLTTTLPTDVTVTSQGTEALPSTWEEQRAAHENLFFTKPLHQVFTSFAKKGEKLDMSLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2U1</accession>
    <name>A0A4W2D2U1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Striatin-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein FAM40A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. The STRIPAK complex can be extended by adapter proteins such as SLMAP:SIKE1, CTTNBP2 or CTTNBP2NL. Interacts with CDC42BPB. Interacts with CTTNBP2NL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2U1"/>
    <dbReference type="SMR" id="A0A4W2D2U1"/>
    <dbReference type="STRING" id="30522.A0A4W2D2U1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034071.1">
        <property type="protein sequence ID" value="ENSBIXP00000020142.1"/>
        <property type="gene ID" value="ENSBIXG00000023506.1"/>
    </dbReference>
    <dbReference type="OMA" id="KMTRAMR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF7">
        <property type="entry name" value="STRIATIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07923">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01293">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01292">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="10">
        <location>
            <begin position="106"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Far11/STRP C-terminal" evidence="11">
        <location>
            <begin position="533"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="409"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="910" mass="103330" checksum="28F8FD7BBA46C0CF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D323</accession>
    <name>A0A4W2D323_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor GATA-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">GATA-binding factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GATA4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSBIXP00005026594.1"/>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 13" id="KW-0862">Zinc</keyword>
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        <location>
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            <end position="265"/>
        </location>
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            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="442" mass="44539" checksum="9C45CF9300DFD270" modified="2019-09-18" version="1">MYQSLAMAANHGPPPGPYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGGGAASGAASGGSSGAAPSGAGPGTQQGSPGWSQAGAEGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGSGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPSGSESLPPATSASSSSSSVATSSSEEMRPIKTEPGLSSHYGPSSSLSQTFSVSAMSGHGSSIHPVLSALKLSPQGYASSVSQSPQASSKQDPWNSLALADSHGDIITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6H9</accession>
    <name>A0A4W2D6H9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Receptor-binding cancer antigen expressed on SiSo cells</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">EBAG9</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.</text>
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        <subcellularLocation>
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        <location>
            <begin position="1"/>
            <end position="16"/>
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            <begin position="17"/>
            <end position="213"/>
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        <source>
            <dbReference type="PIRNR" id="PIRNR034247"/>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="213" mass="24090" checksum="FECF4E16DF28A0FD" modified="2019-09-18" version="1">MAITQFRLFKVCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNALEQLEPDYFKDMTPTIRKTQKIIIKKREPLNFGIPDGSTGFSSRLAATQDMPFIHQSPELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKIAEILGLTMCPSKRERWPPFSSWSFSPPVQTAPIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D701</accession>
    <name>A0A4W2D701_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 7B, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIIb</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase VIIb family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D701"/>
    <dbReference type="STRING" id="30522.A0A4W2D701"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036426.1">
        <property type="protein sequence ID" value="ENSBIXP00000021647.1"/>
        <property type="gene ID" value="ENSBIXG00000024690.1"/>
    </dbReference>
    <dbReference type="OMA" id="CTAIWSY"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01403">
        <property type="entry name" value="Cyt_c_Oxidase_VIIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.51.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7B, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.51.10">
        <property type="entry name" value="Cytochrome C Oxidase, chain K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008433">
        <property type="entry name" value="Cyt_c_oxidase_suVIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023272">
        <property type="entry name" value="Cyt_c_oxidase_suVIIB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16716">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16716:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05392">
        <property type="entry name" value="COX7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81423">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="80" mass="9046" checksum="C4058280C1DF1EF6" modified="2019-09-18" version="1">MFPLAKNALSHLRVQSIQQAVARQIHQKRAPDFHDKYGNAVLASGATFCVAVWVYMATQIGIEWNPSPVGRVTPKEWREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DG76</accession>
    <name>A0A4W2DG76_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DG76"/>
    <dbReference type="STRING" id="30522.A0A4W2DG76"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041662.1">
        <property type="protein sequence ID" value="ENSBIXP00000025131.1"/>
        <property type="gene ID" value="ENSBIXG00000005711.1"/>
    </dbReference>
    <dbReference type="OMA" id="INFTHIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="76"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="247" mass="28532" checksum="874303E426B775A8" modified="2019-09-18" version="1">MDNVEEMDKFLEKYNFPKLDQEEIENLNRPITSTEIETVIRNLPANKSPGPDGFTAEFYQKFREELTPILLKLFQKIAEEGKLPNSFYEATITLIPKPDKDPTKKENYRPISLMNIDAKILNKILAIRIQQHIKKIIHHDQAGFIPGMQGFFNICKSINVIHHINKLKNKNHMIISIDAEKAFDKIQHPFMIRTLQKAGIEGTYFNIIKAIYDKPTANIILNGETLKAFPLKSGTRQGAHFHHYYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DHU2</accession>
    <name>A0A4W2DHU2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 45A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMEM45A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM45 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DHU2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042745.1">
        <property type="protein sequence ID" value="ENSBIXP00000025853.1"/>
        <property type="gene ID" value="ENSBIXG00000027964.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006904">
        <property type="entry name" value="DUF716"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042127">
        <property type="entry name" value="TMEM45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007">
        <property type="entry name" value="EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007:SF21">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04819">
        <property type="entry name" value="DUF716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="275" mass="31592" checksum="6E57B5EB689DCA70" modified="2019-09-18" version="1">MGNFKGHALPGSFFFILGIWWTIKYILKYAFKKQKRTSYLDSKALFHRAEMLEGIVIVGMTLTGILGEQFYPGGPHLTLYDYKEGQWVQLLSWQHFTMYFFFGLLGVTNILSSTVVSLPASFSKLMLSNALFVEAFVFYNHIHGREMLDIFVHKLLVLVVFSTGVIAFMELFAQTSITVELLRTSLFLLQGSWFWQIGFILYPPSGGPAWDLMDHDNVMFLSICFCWHYATAISIVGAIYVFVTWLVKSRFTRFCPSEVGLLKNAEREPESEEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DL40</accession>
    <name>A0A4W2DL40_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TAOK1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027374289.1">
        <property type="nucleotide sequence ID" value="XM_027518488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DL40"/>
    <dbReference type="SMR" id="A0A4W2DL40"/>
    <dbReference type="STRING" id="30522.A0A4W2DL40"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014769.1">
        <property type="protein sequence ID" value="ENSBIXP00000027021.1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
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    <keyword evidence="6" id="KW-0808">Transferase</keyword>
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            <end position="281"/>
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            <position position="58"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1001" mass="116044" checksum="FBE6C55E4B4BCCF7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM83</accession>
    <name>A0A4W2DM83_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 37 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC37A1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Inorganic phosphate and glucose-6-phosphate antiporter. May transport cytoplasmic glucose-6-phosphate into the lumen of the endoplasmic reticulum and translocate inorganic phosphate into the opposite direction. Independent of a lumenal glucose-6-phosphatase. May not play a role in homeostatic regulation of blood glucose levels.</text>
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    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate(in) + phosphate(out) = D-glucose 6-phosphate(out) + phosphate(in)</text>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17344">
        <property type="entry name" value="MFS_SLC37A1_2"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000050">
        <property type="entry name" value="glucose-6-phosphate exchanger SLC37A2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000028">
        <property type="entry name" value="Sugar phosphate exchanger 3 isoform 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="MFS"/>
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    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
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    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044740">
        <property type="entry name" value="SLC37A1_2"/>
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    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF11">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
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    </dbReference>
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        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="150"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="156"/>
            <end position="184"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="215"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="242"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="329"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="423"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="533" mass="57376" checksum="3D151875A3D49368" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNC2</accession>
    <name>A0A4W2DNC2_BOBOX</name>
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        <recommendedName>
            <fullName evidence="24">Germinal-center associated nuclear protein</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">MCM3AP</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.</text>
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    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the SAC3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="5"/>
    <dbReference type="STRING" id="30522.A0A4W2DNC2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000025631.1"/>
        <property type="gene ID" value="ENSBIXG00000027710.1"/>
    </dbReference>
    <dbReference type="OMA" id="DMTIFWH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:nuclear pore"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:transcription export complex 2"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:mRNA export from nucleus"/>
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    </dbReference>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="germinal-center associated nuclear protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031910">
        <property type="entry name" value="GANP_CID_dom"/>
    </dbReference>
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        <property type="entry name" value="MCM3AP_GANP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034265">
        <property type="entry name" value="MCM3AP_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031908">
        <property type="entry name" value="NupH_GANP"/>
    </dbReference>
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        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16766">
        <property type="entry name" value="CID_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCM3AP_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NupH_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="20" id="KW-0012">Acyltransferase</keyword>
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    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="18" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
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        <location>
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            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="198"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="509"/>
            <end position="556"/>
        </location>
    </feature>
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        <location>
            <begin position="1130"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="240"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="291"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038443"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055631"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069544"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025631.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1979" mass="218061" checksum="1188D0309D0FB721" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNN1</accession>
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        <submittedName>
            <fullName evidence="5">N-alpha-acetyltransferase 16, NatA auxiliary subunit</fullName>
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    </protein>
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        <name evidence="5" type="primary">NAA16</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1010:FF:000001">
        <property type="entry name" value="N-alpha-acetyltransferase 15, NatA auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000001">
        <property type="entry name" value="N-alpha-acetyltransferase 15, NatA auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF5">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 16, NATA AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="597"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="863" mass="101280" checksum="7E279244919A6752" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQI2</accession>
    <name>A0A4W2DQI2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TSPY</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQI2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053525.1">
        <property type="protein sequence ID" value="ENSBIXP00000026415.1"/>
        <property type="gene ID" value="ENSBIXG00000030074.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33827" checksum="E8CF8A33121B9CBE" modified="2019-09-18" version="1">ISCPFTSAPARGHCQGQEENERRSEEGRSVPRPRTFLVVSLGTHPTACHPDARLAEDIMEELEVVADEEQEQRSSQELEEKMVEEQDRPGGLNERQALDALQALAALKVELSSECEQNLRAYVQSMHKNHQRRKRHLARRSAIIQGIPGFWAKALSFPHPQVSIMISDQEQDFLGYMIDLKVQVRSHPWSRYKLIFSFRDNPYFLNMVIIKEYYLETTGCRARHSTLVHWFWDFLRGAPSHSRDTRGLNFLNWLSGLNGPESNRSAELIGKDVWDDRLKYYLREEGSSMRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRZ1</accession>
    <name>A0A4W2DRZ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036600.1">
        <property type="protein sequence ID" value="ENSBIXP00000021785.1"/>
        <property type="gene ID" value="ENSBIXG00000024806.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="458" mass="52098" checksum="E9CD96B3EEDB7FB3" modified="2019-09-18" version="1">MPANLENSAVATGLEKVSFHSNPKERQCKECSNYCTIVLISHASQVMLKILQARLQQHVNRELPDVQAGFRKGRGTRDQIANIHWIMEKAREFQKNVYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLNAEYIMRNAGLEEAQGGINIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESENVGLKLNIQKTKIVASGPITSWEIDGETVETVSDFIFGGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERQRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGHLMQRVDSLEKTLMLGGVGGRRRRGPQRMRRLDGITDLMDVSLGEPWELVMDREAWRAAIHGVPKSQTRLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT65</accession>
    <name>A0A4W2DT65_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glucosaminyl (N-acetyl) transferase 2 (I blood group)</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyltransferase 14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT65"/>
    <dbReference type="SMR" id="A0A4W2DT65"/>
    <dbReference type="STRING" id="30522.A0A4W2DT65"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013428.1">
        <property type="protein sequence ID" value="ENSBIXP00000027406.1"/>
        <property type="gene ID" value="ENSBIXG00000000052.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297:SF190">
        <property type="entry name" value="N-ACETYLLACTOSAMINIDE BETA-1,6-N-ACETYLGLUCOSAMINYL-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021473071" evidence="12">
        <location>
            <begin position="18"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="402" mass="45633" checksum="4BCF47E53CC1567A" modified="2019-09-18" version="1">MMGFWKHCLFCVSLVTALIFVFVCNNKLGENKNFLQASLSNASLLSEVCHQIFKGKSFCPKKNALKSTFSETTCHEYVAQSHYITETLSEEEAGFPLAYVMTIHKDFGTFERLFRAIYMPQNVYCVHVDKKATDTFKGSVKQLLSCFPNAFLASKMEPVVYGGISRLQADLNCMKDLVASEVPWKYILNTCGQDFPLKTNREIVQYLKGFKGKNITPGVLPPAHAIGRTKYVHHELLNPKNSYVIKTTKLKTLPPHNMTVYFGTAYVALTREFANFVLHDPLALDLMSWSTDTYSPDEHFWVTLNRIPGVPGSMPNASWAGDLRAVKWLDMEDKHGGCHGHYVHDICIYGNGDLKWLINSSSLFANKFELTAYPLTVECLELRLRERTLNQSEIAIQPSWHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTU2</accession>
    <name>A0A4W2DTU2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hairy and enhancer of split-related protein HELT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HES/HEY-like transcription factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Self-associates. Interacts with HES5 and HEY2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HEY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTU2"/>
    <dbReference type="STRING" id="30522.A0A4W2DTU2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006025.1">
        <property type="protein sequence ID" value="ENSBIXP00000029791.1"/>
        <property type="gene ID" value="ENSBIXG00000011522.1"/>
    </dbReference>
    <dbReference type="OMA" id="GHTHANA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11408">
        <property type="entry name" value="bHLH-O_HELT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000054">
        <property type="entry name" value="hairy and enhancer of split-related protein HELT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="12">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="241" mass="26787" checksum="CDE6B60CABA61909" modified="2019-09-18" version="1">MSDKLKERKRTPVSHKVIEKRRRDRINRCLNELGKTVPMALAKQSSGKLEKAEILEMTVQYLRALHSADFPRGREKAELLAEFANYFHYGYHECMKNLVHYLTTVERMETKDTKYARILAFLQSKARLGTEPAFQPLGSLPEPDFSYQLHPAGPEFAGHSPGEASVFPQGGAGPFAWPHGASRSPALPYLPSAPVPLPSPAPQHSAFLAPGQGLDRHYLNLIGHAHPTALNLHAPQHPPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUG4</accession>
    <name>A0A4W2DUG4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="6">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SIRT3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">NAD-dependent protein deacetylase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DUG4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012203.1">
        <property type="protein sequence ID" value="ENSBIXP00000027811.1"/>
        <property type="gene ID" value="ENSBIXG00000008779.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01408">
        <property type="entry name" value="SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017328">
        <property type="entry name" value="Sirtuin_class_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF5">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037938">
        <property type="entry name" value="SIR2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="11">
        <location>
            <begin position="51"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 10">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="79"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="161"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="253"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="277"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="333" mass="37020" checksum="857C5DA71AF236DA" modified="2019-09-18" version="1">MALFPRSAVPALRFWGLRGARAWTRWPRIPGGGWSISCFAGASSDTGGGDHSQKKFLLQDIAELIKTRACQKVVVMVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWADVMVDRVPRCPVCSGVTKPDIVFFGEPLPARFLLHLADFPMADLLLILGTSLEVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DX80</accession>
    <name>A0A4W2DX80_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PYROXD1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Probable FAD-dependent oxidoreductase; involved in the cellular oxidative stress response. Required for normal sarcomere structure and muscle fiber integrity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Myofibril</location>
            <location evidence="3">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DX80"/>
    <dbReference type="STRING" id="30522.A0A4W2DX80"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051228.1">
        <property type="protein sequence ID" value="ENSBIXP00000023906.1"/>
        <property type="gene ID" value="ENSBIXG00000026214.1"/>
    </dbReference>
    <dbReference type="OMA" id="MCENLIL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000009">
        <property type="entry name" value="Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000165">
        <property type="entry name" value="Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000176">
        <property type="entry name" value="Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000181">
        <property type="entry name" value="Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF2">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18267">
        <property type="entry name" value="Rubredoxin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="15">
        <location>
            <begin position="12"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="15">
        <location>
            <begin position="292"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-rubredoxin oxidoreductase C-terminal" evidence="16">
        <location>
            <begin position="415"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="502" mass="55846" checksum="7ACBB13FB998ACD5" modified="2019-09-18" version="1">MEAPCSPPTAGKFVVVGGGIAGVTCAEQLAINFPSEDILLITASPVIKAVTNFKQVSKVLEEFDVEEQPSTMLENRFPNIKVIESGVKQLKSKEHCVLTEDGNQHIYKKLCLCAGAKPKLICEGNPYVLGIRDTDSAQEFQKQLTKAKRIMIIGNGGIALELVYEIEGCEVIWVIKDKAIGNTFFDAGAAEFLTSKLIDEKPEAKIAQKRTRYTTEGRKKETQARASAGNVGSALGPDWHEGLDLKGAKEFSHKIHIETMCEVKKIYLQEEFRISEKKSLTFPRDHHDQSVTTDKEIWPVYVELTNEKIYGCDFIVSATGVTPNTEPFLCGNNFDVGEDGGLKVDDHMHTSLPDIYAAGDICTAAWHPSPVWQQMRLWTQARQMGWYAAKCMAAASVGESIDMDFSFELFAHVTKFFNYKVVLLGKYNAQGLGSNHELLLRCTKGQEYIKAVLQNGRMMGAVLIGETDLEETFENLILNQMNLSAYGEDLLDPNIDIEDYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2C3</accession>
    <name>A0A4W2E2C3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Leucine rich repeat transmembrane neuronal 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LRRTM4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Postsynaptic cell membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LRRTM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E2C3"/>
    <dbReference type="STRING" id="30522.A0A4W2E2C3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018895.1">
        <property type="protein sequence ID" value="ENSBIXP00000033029.1"/>
        <property type="gene ID" value="ENSBIXG00000002377.1"/>
    </dbReference>
    <dbReference type="OMA" id="LWYCNPN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000005">
        <property type="entry name" value="leucine-rich repeat transmembrane neuronal protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF19">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE NEURONAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021457559" evidence="15">
        <location>
            <begin position="31"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
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        <location>
            <begin position="376"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="590" mass="67341" checksum="1E3AF6D953B01055" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E375</accession>
    <name>A0A4W2E375_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">Homologous recombination OB-fold protein OB-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 19"/>
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        <property type="entry name" value="HOMOLOGOUS RECOMBINATION OB-FOLD PROTEIN"/>
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    </dbReference>
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            <begin position="487"/>
            <end position="570"/>
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            <begin position="256"/>
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        <location>
            <begin position="592"/>
            <end position="628"/>
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        <location>
            <begin position="277"/>
            <end position="298"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15072"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="654" mass="69959" checksum="B8387504C0F993DA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="9 10 11" key="1">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLR_AS"/>
    </dbReference>
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        <property type="entry name" value="GLRX1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLUTAREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46185:SF1">
        <property type="entry name" value="GLUTAREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013662"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="106" mass="11882" checksum="580315C3864AF2F6" modified="2019-09-18" version="1">MAQTFVNSKIQSGKVVVFIKPTCPYCKRTPELLNQLPFKQGLLEFVSITANSDTNEIQDYLQQLTGARTVPRVFVGKECIGGCTDLVNIHERGELLTRIKQIEALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E519</accession>
    <name>A0A4W2E519_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E519"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045266.1">
        <property type="protein sequence ID" value="ENSBIXP00000034042.1"/>
        <property type="gene ID" value="ENSBIXG00000016954.1"/>
    </dbReference>
    <dbReference type="OMA" id="WTTIEER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="512"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="696" mass="80651" checksum="172C2D468D8F113B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6X8</accession>
    <name>A0A4W2E6X8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Potassium channel regulatory protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein CLLD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KCNRG</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Inhibits potassium fluxes in cells. May regulate Kv1 family channel proteins by retaining a fraction of channels in endomembranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Can form homooligomers. Interacts with KCNA1 (via cytoplasmic N-terminal domain) and KCNA4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027413101.1">
        <property type="nucleotide sequence ID" value="XM_027557300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6X8"/>
    <dbReference type="SMR" id="A0A4W2E6X8"/>
    <dbReference type="STRING" id="30522.A0A4W2E6X8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013304.1">
        <property type="protein sequence ID" value="ENSBIXP00000027443.1"/>
        <property type="gene ID" value="ENSBIXG00000008293.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902253"/>
    <dbReference type="CTD" id="283518"/>
    <dbReference type="OMA" id="SYIMDFL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000114">
        <property type="entry name" value="potassium channel regulatory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF5">
        <property type="entry name" value="POTASSIUM CHANNEL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="267" mass="30370" checksum="B3ACF395708FDBA7" modified="2019-09-18" version="1">MSGQELVTLNVGGKVFTTRSSTLQQFPGSRLMRMLDGRDKEFKMVDGQIFVDRDGVLFSFILDFLRTQQLLLPTDFSDHLRLQREALFYELNPLVDLLNQEHKLQPRPALVEVHFLSRNTQAFFRVFGSCSKTIEMLTGRITVFIEQPSAPASSSNSFPQMTLLPLPPQRPSYHDLVFQCGSDSTTDNQTRIRYISIKPDNRKLANGTNVLGLLIDTLLMEGFHLVGTRTVSSEDRTECYSFERIKKPDALAMNKTLKSETTLMPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7M6</accession>
    <name>A0A4W2E7M6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-arrestin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Arrestin beta-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ARRB2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027373893.1">
        <property type="nucleotide sequence ID" value="XM_027518092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E7M6"/>
    <dbReference type="STRING" id="30522.A0A4W2E7M6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016583.1">
        <property type="protein sequence ID" value="ENSBIXP00000032057.1"/>
        <property type="gene ID" value="ENSBIXG00000014531.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877703"/>
    <dbReference type="CTD" id="409"/>
    <dbReference type="OMA" id="TSRHFLM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031701">
        <property type="term" value="F:angiotensin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002031">
        <property type="term" value="P:G protein-coupled receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000003">
        <property type="entry name" value="beta-arrestin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.840:FF:000001">
        <property type="entry name" value="beta-arrestin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000698">
        <property type="entry name" value="Arrestin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017864">
        <property type="entry name" value="Arrestin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014753">
        <property type="entry name" value="Arrestin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792">
        <property type="entry name" value="ARRESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792:SF20">
        <property type="entry name" value="BETA-ARRESTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00309">
        <property type="entry name" value="ARRESTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00295">
        <property type="entry name" value="ARRESTINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="12">
        <location>
            <begin position="194"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="409" mass="46138" checksum="FFFB2522770DCC9C" modified="2019-09-18" version="1">MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIANYQAFPPTPNPPRPPTRLQERLLRKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSLEEKSHKRNSVRLVIRKVQFAPEKPGPQPSAETTRHFLMSDRSLHLEASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQYADICLFSTAQYKCPVAQVEQDDQVSPSSTFCKVYTITPLLSNNREKRGLALDGKLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHIALPRPQSAVPETDAPVDTNLIEFETNYATDDDIVFEDFARLRLKGLKDEDYDDQFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E945</accession>
    <name>A0A4W2E945_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox protein goosecoid-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Homeobox protein goosecoid-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GSC2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May have a role in development. May regulate its own transcription. May bind the bicoid consensus sequence TAATCC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027421835.1">
        <property type="nucleotide sequence ID" value="XM_027566034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E945"/>
    <dbReference type="STRING" id="30522.A0A4W2E945"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024816.1">
        <property type="protein sequence ID" value="ENSBIXP00000035570.1"/>
        <property type="gene ID" value="ENSBIXG00000018867.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022595.1">
        <property type="protein sequence ID" value="ENSBIXP00005012954.1"/>
        <property type="gene ID" value="ENSBIXG00005017143.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907203"/>
    <dbReference type="CTD" id="2928"/>
    <dbReference type="GeneTree" id="ENSGT00940000162536"/>
    <dbReference type="OMA" id="WLDTRFP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000223">
        <property type="entry name" value="Goosecoid homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="119"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="121"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="200" mass="21586" checksum="53A6CFAE4C1D6760" modified="2019-09-18" version="1">MAAARGAAGRGAPGRPCPFSIEHILSGLPERSPQARAARPPPAPDRQSPSEPGEPGAPEAARCACCCCCGPRGPPEADGRLGARLAWPLRLAPAAPLPWAAPSGGPGAQPGAGVQGPPRRTRRHRTIFSEEQLRALEALFMQNQYPDVGTRERLAGRIRLREERVEVWFKNRRAKWRHQKRAAASARLLSGPKKPPRESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E957</accession>
    <name>A0A4W2E957_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multifunctional methyltransferase subunit TRM112-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 112 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TRMT112</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRM112 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E957"/>
    <dbReference type="STRING" id="30522.A0A4W2E957"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010950.1">
        <property type="protein sequence ID" value="ENSBIXP00000028244.1"/>
        <property type="gene ID" value="ENSBIXG00000009344.1"/>
    </dbReference>
    <dbReference type="OMA" id="THNMMAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21089">
        <property type="entry name" value="Trm112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000015">
        <property type="entry name" value="Multifunctional methyltransferase subunit TRM112-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="187" mass="21136" checksum="5DAD5CE3ADF9F3BF" modified="2019-09-18" version="1">MLPGAARDTNRPRLQRAPEVQKFRRFRSSQVPNRIERDSPGNDVERDHSPSGSGFAGAGSVDMRLLTHNLLSSHVRGVGPRGFPLRLQATEVRINPVEFNPDFIVRMIPKVEWAALLEAADHLHLIQVPKEPIQGYEHNEEFLRKMHHVLLEVEVLEGTLQCPESGRVFPISRGIPNMLLSDEETET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9J0</accession>
    <name>A0A4W2E9J0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Voltage-gated inwardly rectifying potassium channel KCNH2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Ether-a-go-go-related gene potassium channel 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Potassium voltage-gated channel subfamily H member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Voltage-gated potassium channel subunit Kv11.1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">KCNH6</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Characterized by unusual gating kinetics by producing relatively small outward currents during membrane depolarization and large inward currents during subsequent repolarization which reflect a rapid inactivation during depolarization and quick recovery from inactivation but slow deactivation (closing) during repolarization. Channel properties are modulated by cAMP and subunit assembly. Forms a stable complex with KCNE1 or KCNE2, and that this heteromultimerization regulates inward rectifier potassium channel activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Interacts with DNAJB12 and DNAJB14; chaperones DNAJB12 and DNAJB14 promote tetramerization. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Interacts with ALG10B. Forms a stable complex with KCNE1 or KCNE2, and that this heteromultimerization regulates Inward rectifier potassium channel activity. Interacts with CANX. The core-glycosylated, but not the fully glycosylated form interacts with RNF207. Interacts with NDFIP1 and NDFIP2; this interaction decreases the cell membrane expression by targeting KCNH2, through interaction with NEDD4L, for the degradation through the multivesicular bodies (MVBs)-lysosomal pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv11.1/KCNH2 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E9J0"/>
    <dbReference type="STRING" id="30522.A0A4W2E9J0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018141.1">
        <property type="protein sequence ID" value="ENSBIXP00000032702.1"/>
        <property type="gene ID" value="ENSBIXG00000015353.1"/>
    </dbReference>
    <dbReference type="OMA" id="FWEDRRP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:inward rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086013">
        <property type="term" value="P:membrane repolarization during cardiac muscle cell action potential"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060307">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane repolarization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000020">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
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    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
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    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
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    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF468">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY H MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
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    </dbReference>
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        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="398"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="491"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="23">
        <location>
            <begin position="594"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="732"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="778"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="858"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="941"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="735"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="754"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00054598"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="971" mass="108347" checksum="93AB933E5AEC7341" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAR6</accession>
    <name>A0A4W2EAR6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hormone-sensitive lipase</fullName>
            <ecNumber evidence="11">3.1.1.23</ecNumber>
            <ecNumber evidence="10">3.1.1.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Monoacylglycerol lipase LIPE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Retinyl ester hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="47" type="primary">LIPE</name>
    </gene>
    <organism evidence="47 48">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="47 48" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="47" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="47" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="42">Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs. Preferentially hydrolyzes fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs and FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O</text>
            <dbReference type="Rhea" id="RHEA:38379"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="34">
            <dbReference type="Rhea" id="RHEA:38381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38455"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:38456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38659"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:38660"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = (9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39935"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:39936"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39939"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:39940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn-glycerol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:34115"/>
            <dbReference type="ChEBI" id="CHEBI:75936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38383"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:38384"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = glycerol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:26133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:38492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolyzes glycerol monoesters of long-chain fatty acids.</text>
            <dbReference type="EC" id="3.1.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>a diacylglycerol + H2O = a monoacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>a monoacylglycerol + H2O = glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13933"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17616"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:13934"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>cholesteryl (9Z-octadecenoate) + H2O = cholesterol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:46898"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:33876"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycerolipid metabolism; triacylglycerol degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="27">Monomer and homodimer. Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <location evidence="4">Caveola</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.23" evidence="11"/>
    <dbReference type="EC" id="3.1.1.79" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EAR6"/>
    <dbReference type="STRING" id="30522.A0A4W2EAR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021145.1">
        <property type="protein sequence ID" value="ENSBIXP00000033978.1"/>
        <property type="gene ID" value="ENSBIXG00000016878.1"/>
    </dbReference>
    <dbReference type="OMA" id="FAACQDH"/>
    <dbReference type="UniPathway" id="UPA00256"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120516">
        <property type="term" value="F:diacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000110">
        <property type="entry name" value="Hormone-sensitive lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000199">
        <property type="entry name" value="Hormone-sensitive lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010468">
        <property type="entry name" value="HSL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06350">
        <property type="entry name" value="HSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="17" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="48" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="22" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Hormone-sensitive lipase N-terminal" evidence="45">
        <location>
            <begin position="53"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="46">
        <location>
            <begin position="392"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="46">
        <location>
            <begin position="703"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="44">
        <location>
            <begin position="584"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="44">
        <location>
            <begin position="627"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="44">
        <location>
            <begin position="627"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="active site" evidence="43">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00013088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00013254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00015845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00031112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00046695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00047476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00047653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00047674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00049053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00049208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00049372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00049461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00049519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00059834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="45">
        <source>
            <dbReference type="Pfam" id="PF06350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="46">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="47">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="48">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="802" mass="88049" checksum="940D7F567BDADCFC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECE4</accession>
    <name>A0A4W2ECE4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Modulator of macroautophagy that causes accumulation of autophagosomes under basal conditions and enhances autophagic flux. Represses cell death and promotes long-term clonogenic survival of cells grown in the absence of glucose in a macroautophagy-independent manner. May have some role in extracellular matrix engulfment or growth factor receptor recycling, both of which can modulate cell survival.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DRAM/TMEM150 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECE4"/>
    <dbReference type="STRING" id="30522.A0A4W2ECE4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045623.1">
        <property type="protein sequence ID" value="ENSBIXP00000034570.1"/>
        <property type="gene ID" value="ENSBIXG00000006544.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF3">
        <property type="entry name" value="MODULATOR OF MACROAUTOPHAGY TMEM150B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="16">
        <location>
            <begin position="110"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="340" mass="37011" checksum="2E379C88FA718CE8" modified="2019-09-18" version="1">MFSAGSPSPPTCSLAVGNWEASVGRIRAPGSRPGPAASRQKRHPAEWAPVGLGSHSLRSVPLVFDRGLWLCRGADSPAPTAQPCSPNSHAAPGPREPGRAWTDPGMWGYLSLLPMCLAFWAIAGIWTVFSLAVVNKAVNLTDGFPYISVCGNVPPQSCIFSQVLNIGAASAAWICILRYYQLRDWGVRKWHNQVILWTGLLCALGTSIVGNFQEKNQRATHLTGAFLAFFVGIVYFWLQLFLSWRMKNLPQPGAPWIGPLRLVLCSACFILEVAMVVLHSWSMRSVSAICEWVAAMLLFILFGLLAVDFSRLDSCTLCLQPGSGSLRPPPDSPTSLHVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDA8</accession>
    <name>A0A4W2EDA8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="5">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SPO11</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027413978.1">
        <property type="nucleotide sequence ID" value="XM_027558177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDA8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028505.1">
        <property type="protein sequence ID" value="ENSBIXP00000037163.1"/>
        <property type="gene ID" value="ENSBIXG00000006813.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050176.1">
        <property type="protein sequence ID" value="ENSBIXP00005020149.1"/>
        <property type="gene ID" value="ENSBIXG00005023438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902800"/>
    <dbReference type="CTD" id="23626"/>
    <dbReference type="GeneTree" id="ENSGT00390000001787"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004084">
        <property type="entry name" value="Meiosis_Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013048">
        <property type="entry name" value="Meiotic_Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03533">
        <property type="entry name" value="SPO11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01551">
        <property type="entry name" value="SPO11HOMOLOG"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Meiosis-specific protein Spo11" evidence="13">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="14">
        <location>
            <begin position="71"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="15">
        <location>
            <begin position="180"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="357" mass="40277" checksum="271F9BE39830FE99" modified="2019-09-18" version="1">MAFTPRGPEASFFEVLDRHRASLLAALRRGGEEPAGGGMRLASRFEDSVGLHMVSHCTTRKIKSDSPKSVKNFALILKIFSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLKVPRRSLHILSTSKGLIAGDLKYLEEDGTKVNCTRATAVAVPSNIQGIQNLITDAKFLLIVEKDATFQRLLDDNFCNKMSPCIMVTGKGVPDVNTRLLIKKLWDTFHIPVFTLVDADPHGIEIMCIYKYGSMAMSFEAHNLTVPAIRWLGLLPSDIKRLNIPKGTLIPLTKRDQMKLDSILKRPYITCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLKFGGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDR3</accession>
    <name>A0A4W2EDR3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal peptide, CUB and EGF-like domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
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</entry>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM171 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF73"/>
    <dbReference type="STRING" id="30522.A0A4W2EF73"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022805.1">
        <property type="protein sequence ID" value="ENSBIXP00000034715.1"/>
        <property type="gene ID" value="ENSBIXG00000017790.1"/>
    </dbReference>
    <dbReference type="OMA" id="PAECMMS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043149">
        <property type="term" value="P:stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018890">
        <property type="entry name" value="FAM171"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049175">
        <property type="entry name" value="FAM171_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048530">
        <property type="entry name" value="FAM171_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31626:SF1">
        <property type="entry name" value="PROTEIN FAM171A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31626">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20771">
        <property type="entry name" value="FAM171A1-2-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10577">
        <property type="entry name" value="FAM171A1-2-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021502818" evidence="10">
        <location>
            <begin position="22"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="FAM171 N-terminal" evidence="11">
        <location>
            <begin position="34"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="FAM171 C-terminal" evidence="12">
        <location>
            <begin position="398"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="736"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="801"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="442"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="864"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="877"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="897" mass="98643" checksum="890BA75CA3551B80" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGM8</accession>
    <name>A0A4W2EGM8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin-8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">STX8</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027374618.1">
        <property type="nucleotide sequence ID" value="XM_027518817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGM8"/>
    <dbReference type="SMR" id="A0A4W2EGM8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001161.1">
        <property type="protein sequence ID" value="ENSBIXP00000031412.1"/>
        <property type="gene ID" value="ENSBIXG00000013666.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026076.1">
        <property type="protein sequence ID" value="ENSBIXP00005015286.1"/>
        <property type="gene ID" value="ENSBIXG00005005438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878018"/>
    <dbReference type="CTD" id="9482"/>
    <dbReference type="GeneTree" id="ENSGT00390000007779"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15852">
        <property type="entry name" value="SNARE_Syntaxin8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000036">
        <property type="entry name" value="Putative Syntaxin-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041875">
        <property type="entry name" value="Syntaxin-8_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF124">
        <property type="entry name" value="SYNTAXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="145"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="236" mass="26760" checksum="5780230741D89500" modified="2019-09-18" version="1">MAPDPWFSTYDSTCQIAQEIAEKIQQRNQYERNGENTTKLTVTIRALLQKLKEKIALLKDLLLRAVATHQITQLEGDRRQNLLDDLVTRERLLLASFKNEGAEPDLIRSSLMTGGAKRGAPNPWLLEEPEETRGLGFDEIRQQQQKIIQEQDAGLDALSSIISRQKQMGQEIGNELDEQNEIIDDLANLVENTDEKLRTETRRVNLVDRKSTSCGMIMVILLLLVAIVVVAVWPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHL4</accession>
    <name>A0A4W2EHL4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EHL4"/>
    <dbReference type="STRING" id="30522.A0A4W2EHL4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028567.1">
        <property type="protein sequence ID" value="ENSBIXP00000037196.1"/>
        <property type="gene ID" value="ENSBIXG00000020720.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033929.1">
        <property type="protein sequence ID" value="ENSBIXP00005020525.1"/>
        <property type="gene ID" value="ENSBIXG00005023622.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015224"/>
    <dbReference type="OMA" id="GIRTICS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000005">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF46">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="103" mass="12138" checksum="15871A55DE0BCACF" modified="2019-09-18" version="1">MKTILSNQTVDIPENVDINLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYIYYYTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHX2</accession>
    <name>A0A4W2EHX2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000016936.1">
        <property type="protein sequence ID" value="ENSBIXP00000032202.1"/>
        <property type="gene ID" value="ENSBIXG00000014694.1"/>
    </dbReference>
    <dbReference type="OMA" id="DCALEPW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000012">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000004">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF468">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1274"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="31"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="137"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="239"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="376"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="476"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="581"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="684"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="789"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="926"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="1031"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="1150"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1338"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1348"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1361"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="175"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="265"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="278"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="401"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="501"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="514"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="606"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="619"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="650"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="722"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="815"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="828"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="859"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="951"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="964"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="995"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1056"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1069"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1175"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1188"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1219"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1448" mass="155652" checksum="B96488CE09DEAAD6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EHX4</accession>
    <name>A0A4W2EHX4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="16">PPIase</shortName>
            <ecNumber evidence="16">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PPIA</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21 22">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="16"/>
    <dbReference type="RefSeq" id="XP_027395066.1">
        <property type="nucleotide sequence ID" value="XM_027539265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EHX4"/>
    <dbReference type="SMR" id="A0A4W2EHX4"/>
    <dbReference type="STRING" id="30522.A0A4W2EHX4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026565.1">
        <property type="protein sequence ID" value="ENSBIXP00000036325.1"/>
        <property type="gene ID" value="ENSBIXG00000019767.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024663.1">
        <property type="protein sequence ID" value="ENSBIXP00005014347.1"/>
        <property type="gene ID" value="ENSBIXG00005018352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891341"/>
    <dbReference type="CTD" id="5478"/>
    <dbReference type="GeneTree" id="ENSGT00950000183087"/>
    <dbReference type="OMA" id="CVSIYGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000011">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF490">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="17">
        <location>
            <begin position="7"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="164" mass="17869" checksum="F8C9D12484EF44B6" modified="2019-09-18" version="1">MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFDDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EK17</accession>
    <name>A0A4W2EK17_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box only protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FBXO32</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027416709.1">
        <property type="nucleotide sequence ID" value="XM_027560908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EK17"/>
    <dbReference type="STRING" id="30522.A0A4W2EK17"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045254.1">
        <property type="protein sequence ID" value="ENSBIXP00000034039.1"/>
        <property type="gene ID" value="ENSBIXG00000016942.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040193.1">
        <property type="protein sequence ID" value="ENSBIXP00005024793.1"/>
        <property type="gene ID" value="ENSBIXG00005027349.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904130"/>
    <dbReference type="CTD" id="114907"/>
    <dbReference type="GeneTree" id="ENSGT00390000004915"/>
    <dbReference type="OMA" id="NINTWVH"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071549">
        <property type="term" value="P:cellular response to dexamethasone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014894">
        <property type="term" value="P:response to denervation involved in regulation of muscle adaptation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22103">
        <property type="entry name" value="F-box_FBXO32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000017">
        <property type="entry name" value="F-box only protein 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040394">
        <property type="entry name" value="FBX25/32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123:SF6">
        <property type="entry name" value="F-BOX ONLY PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123">
        <property type="entry name" value="LD30288P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="10">
        <location>
            <begin position="226"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="355" mass="41595" checksum="C237131A1B23D2C4" modified="2019-09-18" version="1">MPFLGQDWRSPGQSWVKTADGWKRFLDEKSGSFVSDLGSYCSKEVYNKENLFNSLNYDVAAKKRKKDMLNSKTKTQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSTAILDSRRFNYVVRLLELIAKSQLTSLSGIAQKNFMNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETILHWQQQLNNIQITRPAFKGLTFTDLPLCLQLNIMQRLSDGRDLVSLGQVAPDLHVLSEDRLLWKKLCQYHFSERQIRKRLILSDKGQLDWKKMYFKLARCYPRKEQYGDTLQLCRHCHILSWKGTDHPCTANNPESCSISLSPQDFINLFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EM10</accession>
    <name>A0A4W2EM10_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CBFA2/RUNX1 partner transcriptional co-repressor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CBFA2T2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CBFA2T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414590.1">
        <property type="nucleotide sequence ID" value="XM_027558789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EM10"/>
    <dbReference type="SMR" id="A0A4W2EM10"/>
    <dbReference type="STRING" id="30522.A0A4W2EM10"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029654.1">
        <property type="protein sequence ID" value="ENSBIXP00000037665.1"/>
        <property type="gene ID" value="ENSBIXG00000021283.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041535.1">
        <property type="protein sequence ID" value="ENSBIXP00005044323.1"/>
        <property type="gene ID" value="ENSBIXG00005028172.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903115"/>
    <dbReference type="CTD" id="9139"/>
    <dbReference type="GeneTree" id="ENSGT00950000183176"/>
    <dbReference type="OMA" id="WKHLDHX"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000001">
        <property type="entry name" value="CBFA2/RUNX1 translocation partner 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000001">
        <property type="entry name" value="RUNX1 translocation partner 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013291">
        <property type="entry name" value="MTGR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014896">
        <property type="entry name" value="NHR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF13">
        <property type="entry name" value="PROTEIN CBFA2T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08788">
        <property type="entry name" value="NHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01875">
        <property type="entry name" value="ETOFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01877">
        <property type="entry name" value="MTGR1PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAFH" evidence="13">
        <location>
            <begin position="104"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="12">
        <location>
            <begin position="498"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="220"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="388"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="538"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="538"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="574"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="595" mass="65831" checksum="23369842A7A3A4A9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EM99</accession>
    <name>A0A4W2EM99_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Iron-binding zinc finger CDGSH type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EM99"/>
    <dbReference type="STRING" id="30522.A0A4W2EM99"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030269.1">
        <property type="protein sequence ID" value="ENSBIXP00000037936.1"/>
        <property type="gene ID" value="ENSBIXG00000004129.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034820.1">
        <property type="protein sequence ID" value="ENSBIXP00005041204.1"/>
        <property type="gene ID" value="ENSBIXG00005004132.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154445"/>
    <dbReference type="OMA" id="CHRWRSK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.90:FF:000001">
        <property type="entry name" value="CDGSH iron-sulfur domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045131">
        <property type="entry name" value="CISD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019610">
        <property type="entry name" value="FeS-contain_mitoNEET_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680:SF5">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10660">
        <property type="entry name" value="MitoNEET_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="12">
        <location>
            <begin position="61"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="112" mass="12743" checksum="B9ED827336F3C638" modified="2019-09-18" version="1">MDVIRNKLELSTCHTKVEWIAAVTIAAGTAATGYPAYKRFYVKDHHNKSMVNPHIQKDNPKVVHAFDMEDLGDKAVYCHSWRFKKFPLCDGSHTKHNEETGDNMGPLTNRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMK3</accession>
    <name>A0A4W2EMK3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DSN1 component of MIS12 kinetochore complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DSN1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027414409.1">
        <property type="nucleotide sequence ID" value="XM_027558608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMK3"/>
    <dbReference type="SMR" id="A0A4W2EMK3"/>
    <dbReference type="STRING" id="30522.A0A4W2EMK3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030482.1">
        <property type="protein sequence ID" value="ENSBIXP00000038009.1"/>
        <property type="gene ID" value="ENSBIXG00000007528.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005631.1">
        <property type="protein sequence ID" value="ENSBIXP00005031151.1"/>
        <property type="gene ID" value="ENSBIXG00005011519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903061"/>
    <dbReference type="CTD" id="79980"/>
    <dbReference type="GeneTree" id="ENSGT00390000011347"/>
    <dbReference type="OMA" id="QDKSQSW"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:fibrillar center"/>
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        <property type="term" value="C:nuclear body"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cell division"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:skeletal muscle satellite cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Dsn1/Mis13"/>
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        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778:SF2">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08202">
        <property type="entry name" value="MIS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="353" mass="40233" checksum="9566DF66433FDACC" modified="2019-09-18" version="1">MTSRPEIVKVLEEEPVASKTHDRQLESDPNPVEVCNKSSASLKRTERVSKERIHLGRSPPKGESGDLSHQEGLESRSFHLSPEQSPCHQARRQSWRRASMKETNRRKSLPPFHQGITELCRSISVDLTESKRLSSLLLSSFQFSVQKLEPFLRGTEGFSLESFRAKASSLSEELKHFADKLESNGTLQKCFEDSEGRVSDLALETSVAEMKEYITKFSLERQSWDQLLLRYQEEAEKILSRGSAETKNTEVEVEPRMYVGSSQSEVLNTKPDYQKILQNQTKLFDCMELVMDELQGSVKQLHAFMDESTQCLQEVSVQLGKRSTQQLDPSPARKLLKLQLQKQPSTYCSKSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EN18</accession>
    <name>A0A4W2EN18_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0690 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EN18"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048236.1">
        <property type="protein sequence ID" value="ENSBIXP00000038136.1"/>
        <property type="gene ID" value="ENSBIXG00000021897.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029089">
        <property type="entry name" value="DUF4660"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31833">
        <property type="entry name" value="UPF0690 PROTEIN C1ORF52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31833:SF2">
        <property type="entry name" value="UPF0690 PROTEIN C1ORF52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15559">
        <property type="entry name" value="DUF4660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="257" mass="27590" checksum="B267C83B64B06BC7" modified="2019-09-18" version="1">MSIRTPCLGTCGQPSASVPEAGRKNATQPPGGADLGPVSETWGGVSCGAGPGVTSGFRRSRGRTGAAVMAAEEKDPLSYFAAYGSSSSGSSDEEDNSEPEETSRKGQDTAKSAGGYGNKAEKRLPGPDELFRSVTRPAFLYNPLNKQIDWERHVVKAPEEPPKEFKIWKSHYVPPPETYSTEKKPPPPELDMAIKWSNIYEDNGDDAPQNAKKARLLPEGEETVESGGEEACFFLPLEYSDSPGNGGESRTTYIGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERX3</accession>
    <name>A0A4W2ERX3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1</fullName>
            <ecNumber evidence="5">2.4.99.20</ecNumber>
            <ecNumber evidence="4">3.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">2'-phospho-ADP-ribosyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">2'-phospho-cyclic-ADP-ribose transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">ADP-ribosyl cyclase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cyclic ADP-ribose hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.20" evidence="5"/>
    <dbReference type="EC" id="3.2.2.6" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ERX3"/>
    <dbReference type="SMR" id="A0A4W2ERX3"/>
    <dbReference type="STRING" id="30522.A0A4W2ERX3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034343.1">
        <property type="protein sequence ID" value="ENSBIXP00000039715.1"/>
        <property type="gene ID" value="ENSBIXG00000023657.1"/>
    </dbReference>
    <dbReference type="OMA" id="SLYDCEE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04759">
        <property type="entry name" value="Rib_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF5">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021258199" description="ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1" evidence="23">
        <location>
            <begin position="27"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="278" mass="31546" checksum="8AA7996D4434B9A1" modified="2019-09-18" version="1">MADCCTPRKKLCWCVLLFFLVAGITGGAIGIYKWHYSGLNRWHGAGSTADFQKIIQERCDTYTQTIRPGSRSRNCQAIRQAFMSAFISKDPCKATKEDYNSLINLAPPTVPCGQQVFWSKTKELAHEYAKRRRLMTLEDTLLGYLADGLRWCGEPGSSDLNIWSCPDWRKDCRTNYLSVFWEVLSERFAESACNTVRVVLNGSLENAFDSMSIFGRVEAPNLRPQVELEAWLVHDTGKPPSDSCSGSSIRKLKSILDGRNVKFRCMDNLSRDQFLQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV02</accession>
    <name>A0A4W2EV02_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenomatous polyposis coli protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Stabilizes microtubules and may regulate actin fiber dynamics through the activation of Rho family GTPases. May also function in Wnt signaling by promoting the rapid degradation of CTNNB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenomatous polyposis coli (APC) family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EV02"/>
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        <property type="protein sequence ID" value="ENSBIXP00000040839.1"/>
        <property type="gene ID" value="ENSBIXG00000024999.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045295">
        <property type="term" value="F:gamma-catenin binding"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cell fate specification"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
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        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
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        <property type="term" value="P:nervous system development"/>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="APC_basic_dom"/>
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        <property type="entry name" value="APC_dom"/>
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        <property type="entry name" value="Apc_fam"/>
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        <property type="entry name" value="APC_rpt"/>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
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        <property type="entry name" value="SAMP"/>
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    <dbReference type="PANTHER" id="PTHR12607:SF3">
        <property type="entry name" value="ADENOMATOUS POLYPOSIS COLI PROTEIN 2"/>
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    </dbReference>
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        <property type="entry name" value="ADENOMATOUS POLYPOSIS COLI PROTEIN FAMILY"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Arm_APC_u3"/>
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    <dbReference type="Pfam" id="PF11414">
        <property type="entry name" value="Suppressor_APC"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00185">
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
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    <dbReference type="SUPFAM" id="SSF58050">
        <property type="entry name" value="N-terminal coiled coil domain from apc"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF82931">
        <property type="entry name" value="Tumor suppressor gene product Apc"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
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    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
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    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Adenomatous polyposis coli N-terminal dimerisation" evidence="17">
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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    <protein>
        <recommendedName>
            <fullName evidence="4 11">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit</fullName>
            <shortName evidence="11">Oligosaccharyl transferase 48 kDa subunit</shortName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Required for the assembly of both SST3A- and SS3B-containing OST complexes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Interacts with SMIM22.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EXL1"/>
    <dbReference type="SMR" id="A0A4W2EXL1"/>
    <dbReference type="STRING" id="30522.A0A4W2EXL1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038558.1">
        <property type="protein sequence ID" value="ENSBIXP00000041552.1"/>
        <property type="gene ID" value="ENSBIXG00000025687.1"/>
    </dbReference>
    <dbReference type="OMA" id="AHDEYPR"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="13">
        <location>
            <begin position="135"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="14">
        <location>
            <begin position="402"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="545" mass="60352" checksum="1F00B1A3E1D93C3C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0I5</accession>
    <name>A0A4W2F0I5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17 21">Enteropeptidase</fullName>
            <ecNumber evidence="16 21">3.4.21.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 21">Enterokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 21">Serine protease 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 21">Transmembrane protease serine 15</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15 21">Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 21">
            <text>Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.</text>
            <dbReference type="EC" id="3.4.21.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 21">Membrane</location>
            <topology evidence="1 21">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DMBT1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the peptidase S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.9" evidence="16 21"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0I5"/>
    <dbReference type="STRING" id="30522.A0A4W2F0I5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041049.1">
        <property type="protein sequence ID" value="ENSBIXP00000042868.1"/>
        <property type="gene ID" value="ENSBIXG00000026861.1"/>
    </dbReference>
    <dbReference type="OMA" id="THGICNG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000043">
        <property type="entry name" value="Enteropeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000029">
        <property type="entry name" value="Enteropeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.960:FF:000007">
        <property type="entry name" value="Enteropeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000144">
        <property type="entry name" value="enteropeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000128">
        <property type="entry name" value="enteropeptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000038">
        <property type="entry name" value="enteropeptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000149">
        <property type="entry name" value="enteropeptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011163">
        <property type="entry name" value="Pept_S1A_enterop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF16">
        <property type="entry name" value="TRANSMEMBRANE SERINE PROTEASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001138">
        <property type="entry name" value="Enteropeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
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        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="12 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 21 25" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 21 26" id="KW-0472">Membrane</keyword>
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    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 21 25" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
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        <location>
            <begin position="21"/>
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        <location>
            <begin position="54"/>
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            <begin position="225"/>
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        <location>
            <begin position="346"/>
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        <location>
            <begin position="525"/>
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        <location>
            <begin position="679"/>
            <end position="789"/>
        </location>
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    <feature type="domain" description="Peptidase S1" evidence="30">
        <location>
            <begin position="786"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="826"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="877"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="972"/>
        </location>
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    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="644"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="651"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="663"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009931"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050263"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066389"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
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    <evidence type="ECO:0000256" key="18">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1020" mass="113380" checksum="372E5CC85F856F74" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1C1</accession>
    <name>A0A4W2F1C1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Non-selective voltage-gated ion channel VDAC2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel adopts an open conformation at zero mV and a closed conformation at both positive and negative potentials. There are two populations of channels; the main that functions in a lower open-state conductance with lower ion selectivity, that switch, in a voltage-dependent manner, from the open to a low-conducting 'closed' state and the other that has a normal ion selectivity in the typical high conductance, 'open' state. Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol. Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer, homodimer and higher order oligomers; formation of higher order structures is necessary for scramblase activity. Interacts with ARMC12 in a TBC1D21-dependent manner. Interacts with KLC3. Interacts with SPATA33. Interacts with PPP3CC in a SPATA33-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000027223.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005040463.1"/>
        <property type="gene ID" value="ENSBIXG00005023153.1"/>
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        <property type="component" value="Chromosome 5"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent anion-selective channel protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Porin_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF12">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0626">Porin</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049967"/>
        </source>
    </evidence>
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        <source>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ruminantia</taxon>
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        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <text>O-phospho-L-seryl-[myosin light chain] + H2O = L-seryl-[myosin light chain] + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:13684"/>
            <dbReference type="Rhea" id="RHEA-COMP:13685"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 23">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-threonyl-[myosin light chain] + H2O = L-threonyl-[myosin light chain] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:53988"/>
            <dbReference type="Rhea" id="RHEA-COMP:13686"/>
            <dbReference type="Rhea" id="RHEA-COMP:13687"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 23">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="17"/>
    <dbReference type="RefSeq" id="XP_027411564.1">
        <property type="nucleotide sequence ID" value="XM_027555763.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027411565.1">
        <property type="nucleotide sequence ID" value="XM_027555764.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027411566.1">
        <property type="nucleotide sequence ID" value="XM_027555765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F7R1"/>
    <dbReference type="SMR" id="A0A4W2F7R1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048406.1">
        <property type="protein sequence ID" value="ENSBIXP00000018310.1"/>
        <property type="gene ID" value="ENSBIXG00000004303.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015406.1">
        <property type="protein sequence ID" value="ENSBIXP00005000830.1"/>
        <property type="gene ID" value="ENSBIXG00005029781.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901426"/>
    <dbReference type="CTD" id="5500"/>
    <dbReference type="GeneTree" id="ENSGT00940000154644"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072357">
        <property type="term" value="C:PTW/PP1 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050115">
        <property type="term" value="F:myosin-light-chain-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000007">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF472">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP1-BETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="19">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="327" mass="37187" checksum="E8356022E9B94ECD" modified="2019-09-18" version="1">MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FH25</accession>
    <name>A0A4W2FH25_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Angiopoietin like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ANGPTL4</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027403327.1">
        <property type="nucleotide sequence ID" value="XM_027547526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FH25"/>
    <dbReference type="SMR" id="A0A4W2FH25"/>
    <dbReference type="STRING" id="30522.A0A4W2FH25"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048597.1">
        <property type="protein sequence ID" value="ENSBIXP00000038619.1"/>
        <property type="gene ID" value="ENSBIXG00000006068.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008559.1">
        <property type="protein sequence ID" value="ENSBIXP00005004304.1"/>
        <property type="gene ID" value="ENSBIXG00005010288.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895826"/>
    <dbReference type="CTD" id="51129"/>
    <dbReference type="GeneTree" id="ENSGT00940000159478"/>
    <dbReference type="OMA" id="MATGFPF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035473">
        <property type="term" value="F:lipase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055102">
        <property type="term" value="F:lipase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072577">
        <property type="term" value="P:endothelial cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000352">
        <property type="term" value="P:negative regulation of endothelial cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010903">
        <property type="term" value="P:negative regulation of very-low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090318">
        <property type="term" value="P:regulation of chylomicron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.530.10:FF:000001">
        <property type="entry name" value="angiopoietin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611356" evidence="10">
        <location>
            <begin position="24"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="11">
        <location>
            <begin position="186"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="109"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="410" mass="45553" checksum="932B5F43B268EC19" modified="2019-09-18" version="1">MRCAPTAGAALMLCAATAGLLSAQGRPEPPETPRFASWDEVNVLAHGLLQLGHGLREHVERTRGQLGELERRLGACGAACKDPEGSAAPPRAQANLVNPGGGDASPETLRSLKTQLEAQNSRIQQLFQKVAQQQRHLEKQQLRIQNLQSQMDHLAPRHLGHEMAKPARRKRLPKMAQLAGPAHNISRLHRLPRDCQELFEEGERESGLFQIQPQGSPPFLVNCKMTSDGGWTVIQRRQDGSVDFNQPWEAYKDGFGDPQGEFWLGLEKVHHILGDRGSRLAVQLQDWEGNAESLQFPIHLGGEDTAYSLQLTPPVASKLGATTFSPSGLSLPFSTWDQDHDLRGDKNCARSLSGGWWFGTCSHSNLNGQYFHSIPRQRQQRKKGIFWKTWRGRYYPLQATTILVQPTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FPR8</accession>
    <name>A0A4W2FPR8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cyclin-dependent kinase 4</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cell division protein kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CDK6</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027395982.1">
        <property type="nucleotide sequence ID" value="XM_027540181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FPR8"/>
    <dbReference type="SMR" id="A0A4W2FPR8"/>
    <dbReference type="STRING" id="30522.A0A4W2FPR8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037234.1">
        <property type="protein sequence ID" value="ENSBIXP00000022214.1"/>
        <property type="gene ID" value="ENSBIXG00000025139.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001843.1">
        <property type="protein sequence ID" value="ENSBIXP00005007696.1"/>
        <property type="gene ID" value="ENSBIXG00005012910.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891736"/>
    <dbReference type="CTD" id="1021"/>
    <dbReference type="GeneTree" id="ENSGT00940000157957"/>
    <dbReference type="OMA" id="YEEHRVI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097131">
        <property type="term" value="C:cyclin D1-CDK6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097132">
        <property type="term" value="C:cyclin D2-CDK6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097133">
        <property type="term" value="C:cyclin D3-CDK6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098770">
        <property type="term" value="F:FBXO family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043697">
        <property type="term" value="P:cell dedifferentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042063">
        <property type="term" value="P:gliogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000773">
        <property type="term" value="P:negative regulation of cellular senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050680">
        <property type="term" value="P:negative regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045656">
        <property type="term" value="P:negative regulation of monocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045668">
        <property type="term" value="P:negative regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001954">
        <property type="term" value="P:positive regulation of cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048146">
        <property type="term" value="P:positive regulation of fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045646">
        <property type="term" value="P:regulation of erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902036">
        <property type="term" value="P:regulation of hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033077">
        <property type="term" value="P:T cell differentiation in thymus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003323">
        <property type="term" value="P:type B pancreatic cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07862">
        <property type="entry name" value="STKc_CDK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000124">
        <property type="entry name" value="Cyclin-dependent kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000205">
        <property type="entry name" value="Cyclin-dependent kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028788">
        <property type="entry name" value="CDK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF130">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="13"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="19"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19 20">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
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        <source>
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            <dbReference type="PIRSR" id="PIRSR628788-2"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="326" mass="37014" checksum="04A5A3F1AC71BB04" modified="2019-09-18" version="1">MEKDGLSRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRLAFHSKSPQPIEKFVTDIDEQGKDLLLKCLTFNPAKRISAYSALSHPYFHDLERRKENLDSHLPPSQNSSEMNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FUH3</accession>
    <name>A0A4W2FUH3_BOBOX</name>
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        <submittedName>
            <fullName evidence="11">Calcium homeostasis modulator 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CALHM3</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027529277.1"/>
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        <property type="project" value="TreeGrafter"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="344" mass="38602" checksum="B84CEFFAA7F24424" modified="2019-09-18" version="1">MDKFRTLFLHFQSSSESVMNGVCLLLAMVTVKLYSSFDFNCPCLARYNALYGLGLLFTPPIALFLCGLLANRQSVVMVEEWRRPAGHRRKDPGIVRYMCSSVLQRALAAPVIWILLALLDGKCFVCAFSSSVDPEKFLDFTNMTPSQVQLFLAKVPCKEDELVRNSPARKAVSRYLRCLSQAIGWSLTLLLILLAFLARCLRPCFDQTVFLQRRYWSNYVDLEQKLFDETCCEHARDFAHRCVLHFFASMQSEMQARGLRRDSAGRGPEAPERPEPPDGLDGGSGKAHLRAVSSQEQVDRLLSAWYSSKPPLDLTASSGLWGCGLTHRAPTVVPGTRESPHTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G0B1</accession>
    <name>A0A4W2G0B1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sphingosine 1-phosphate receptor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Endothelial differentiation G-protein coupled receptor 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sphingosine 1-phosphate receptor Edg-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">S1PR4</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027547176.1"/>
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    <dbReference type="STRING" id="30522.A0A4W2G0B1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033690.1">
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        <property type="gene ID" value="ENSBIXG00000007209.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005020018.1">
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT01050000244887"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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    </dbReference>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
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            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="205"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
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        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="64"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="385" mass="41787" checksum="F3CE1B9222E89740" modified="2019-09-18" version="1">MNATGTPTGDPESCQLLAAGGHSRLIVLHYNHSGRLAGRAGAEEVGLGVLRGLFVAVSCLVVLENMLVLVAIASRMRSRRWVYYCLVNITLSDLLTGAAYLANVLLSGAHTFRLAPSQWFLREGLLFMALAASTFSLLFTAGERFATMVRPVAESGATKRGRVCSFIGLCWLLAALLGLLPLLGWNCVCAFQRCSSLLPLYSKDYILFCVVVFACILATIMVLYGAIFRVVRANGQKAPSIPARRKAQRLLKTVLMILVAFVVCWGPLFGLLLADIFGSNVWAQEYLRGMDWILALAVLNSAVNPIIYSFQSREVCRAVVGFLCGACLRLGLRGPGDCLAQAVEAHSAASNTDSSLRPRDSFRGSRSHSFRMREPLTSVSSVRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GHH5</accession>
    <name>A0A4W2GHH5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">G protein subunit alpha 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GNA13</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375008.1">
        <property type="nucleotide sequence ID" value="XM_027519207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GHH5"/>
    <dbReference type="SMR" id="A0A4W2GHH5"/>
    <dbReference type="STRING" id="30522.A0A4W2GHH5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045370.1">
        <property type="protein sequence ID" value="ENSBIXP00000012003.1"/>
        <property type="gene ID" value="ENSBIXG00000017144.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029959.1">
        <property type="protein sequence ID" value="ENSBIXP00005017878.1"/>
        <property type="gene ID" value="ENSBIXG00005021343.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878233"/>
    <dbReference type="CTD" id="10672"/>
    <dbReference type="GeneTree" id="ENSGT00940000157054"/>
    <dbReference type="OMA" id="RFACMRC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031752">
        <property type="term" value="F:D5 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:branching involved in blood vessel morphogenesis"/>
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        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
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        <property type="term" value="P:platelet activation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of blood pressure"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:regulation of fibroblast migration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of postsynapse assembly"/>
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    </dbReference>
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        <property type="term" value="P:Rho protein signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160221">
        <property type="term" value="P:Rho-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000469">
        <property type="entry name" value="Gprotein_alpha_12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF85">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="58"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="197"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="222"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010405"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="377" mass="43946" checksum="9122418B143C5845" modified="2019-09-18" version="1">MADFLPSRSVLSVCFPGCVLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQLNGDKLMAFDTRTPLTAQGIVETQVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPEYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRSKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GHK5</accession>
    <name>A0A4W2GHK5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CLN8 transmembrane ER and ERGIC protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CLN8</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GHK5"/>
    <dbReference type="STRING" id="30522.A0A4W2GHK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004376.1">
        <property type="protein sequence ID" value="ENSBIXP00000030329.1"/>
        <property type="gene ID" value="ENSBIXG00000012265.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029411.1">
        <property type="protein sequence ID" value="ENSBIXP00005017519.1"/>
        <property type="gene ID" value="ENSBIXG00005021015.1"/>
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    <dbReference type="OMA" id="FFRTFDL"/>
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        <property type="component" value="Chromosome 27"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0097001">
        <property type="term" value="F:ceramide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007628">
        <property type="term" value="P:adult walking behavior"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008306">
        <property type="term" value="P:associative learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0051935">
        <property type="term" value="P:glutamate reuptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:lysosome organization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007006">
        <property type="term" value="P:mitochondrial membrane organization"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:musculoskeletal movement"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0060052">
        <property type="term" value="P:neurofilament cytoskeleton organization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050884">
        <property type="term" value="P:neuromuscular process controlling posture"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097473">
        <property type="term" value="P:retinal rod cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035176">
        <property type="term" value="P:social behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021523">
        <property type="term" value="P:somatic motor neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
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        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF7">
        <property type="entry name" value="PROTEIN CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="60"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="339" mass="37445" checksum="383E01B1AB9AF71E" modified="2019-09-18" version="1">MGSMSLLGAGALDLDYTSGRVRGVLVAAGCAFYLGVFVVCHRLSSSLNATYRSLAAREQVFWNLAATRAVFGIQGTAAGLWALLLDPVLQADKALGQQDWCWFHITTATGFFFFENLALHVSNALFHTFDGFLAVHHLFAFLGFLGSAVNLQAGHYLPMVTLLLEMSTPFTCISWMLLKAGCANSRLWRLNQWAMVHLFHCRMVLTYHMWWVCLGHWAGLARSLFPPHLALFVVGLALLTLLINPYWTRKKTQQLLNPVDWNFAPGTPNGPAQPKKARDPGGRGCFHRPELESSDLSSQNLKARPCVSLSAVHLAAEVFHLGDAAVMISLHVLKTQVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GNM1</accession>
    <name>A0A4W2GNM1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GNM1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052023.1">
        <property type="protein sequence ID" value="ENSBIXP00000043207.1"/>
        <property type="gene ID" value="ENSBIXG00000005718.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032434.1">
        <property type="protein sequence ID" value="ENSBIXP00005019523.1"/>
        <property type="gene ID" value="ENSBIXG00005003887.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282489"/>
    <dbReference type="OMA" id="SEYMECE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="236" mass="26770" checksum="34D38CC256421507" modified="2019-09-18" version="1">MESQRFSWSQTPTPVLSLQDPLKNHLPMIMEKAREFQKNIYFCFIDYAKAFDCVDHKKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVHQGCILSPCLFNLYAEYIMRNTGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELNSLLMKVKEESEKAGLKLNIQKTKIMASGPITSWEIDGETVSDFILGGSKITAGGDCSHEIKKMLTPWKESYDQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GUU4</accession>
    <name>A0A4W2GUU4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polycomb group RING finger protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PCGF5</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity. Plays a redundant role with PCGF3 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of a PRC1-like complex that contains PCGF5, RNF2 and UBE2D3. Interacts with RNF2; the interaction is direct. Interacts with CBX6, CBX7 and CBX8. Interacts with AUTS2; the interaction is direct. Identified in a complex that contains AUTS2, PCGF5, CSNK2B and RNF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027385290.1">
        <property type="nucleotide sequence ID" value="XM_027529489.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385291.1">
        <property type="nucleotide sequence ID" value="XM_027529490.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385292.1">
        <property type="nucleotide sequence ID" value="XM_027529491.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385293.1">
        <property type="nucleotide sequence ID" value="XM_027529492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GUU4"/>
    <dbReference type="STRING" id="30522.A0A4W2GUU4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001826.1">
        <property type="protein sequence ID" value="ENSBIXP00000007451.1"/>
        <property type="gene ID" value="ENSBIXG00000013394.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035440.1">
        <property type="protein sequence ID" value="ENSBIXP00005021529.1"/>
        <property type="gene ID" value="ENSBIXG00005024540.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884677"/>
    <dbReference type="CTD" id="84333"/>
    <dbReference type="GeneTree" id="ENSGT00940000155896"/>
    <dbReference type="OMA" id="GENGYPM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
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        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:PcG protein complex"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000805">
        <property type="term" value="C:X chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140862">
        <property type="term" value="F:histone H2AK119 ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:random inactivation of X chromosome"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="RAWUL_PCGF5"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd16737">
        <property type="entry name" value="RING-HC_PCGF5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="polycomb group RING finger protein 5 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative polycomb group RING finger protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PcG_RING_finger"/>
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    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45893">
        <property type="entry name" value="POLYCOMB GROUP RING FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="97"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059948"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="256" mass="29771" checksum="CF948A84CCFCE4E6" modified="2019-09-18" version="1">MATQRKHLVKDFNPYITCYICKGYLIKPTTVTECLHTFCKTCIVQHFEDSNDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELERESEFWKKNKPQENGQDDMSKVDKPKVDEEGDENQDDKDYHRSDPQIAICLDCLRNNGQSGDNVVKGLMKKFIRCSTRVTVGTIKKFLSLKLKLPSSYELDVLCNGEIMGKDHTMEFIYMTRWRLRGENFRCLNCSASQVCSQDGPLYQSYPMVLQYRPRIDFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GX80</accession>
    <name>A0A4W2GX80_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Gamma-aminobutyric acid type A receptor subunit beta3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GABRB3</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376614.1">
        <property type="nucleotide sequence ID" value="XM_027520813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GX80"/>
    <dbReference type="SMR" id="A0A4W2GX80"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017382.1">
        <property type="protein sequence ID" value="ENSBIXP00000009245.1"/>
        <property type="gene ID" value="ENSBIXG00000014962.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036510.1">
        <property type="protein sequence ID" value="ENSBIXP00005022059.1"/>
        <property type="gene ID" value="ENSBIXG00005025037.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879432"/>
    <dbReference type="CTD" id="2562"/>
    <dbReference type="GeneTree" id="ENSGT00940000154713"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902711">
        <property type="term" value="C:GABA-A receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18999">
        <property type="entry name" value="LGIC_ECD_GABAAR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19053">
        <property type="entry name" value="LGIC_TM_GABAAR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000004">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000004">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516540" evidence="19">
        <location>
            <begin position="23"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="39"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="250"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="472" mass="54224" checksum="7969308CB6480F13" modified="2019-09-18" version="1">MCSGLPAILLPIWLSWTLGTRGSEPRSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKNAKAKNDRSKGDSNRVDAHGNILLTSLEVHNEMNEVAGGVGDTRNSARSFDNSGIQYRKQSMPREGHGRHMDRSVPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GZU7</accession>
    <name>A0A4W2GZU7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-defensin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DEFB114</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Has antibacterial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the beta-defensin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GZU7"/>
    <dbReference type="SMR" id="A0A4W2GZU7"/>
    <dbReference type="STRING" id="30522.A0A4W2GZU7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006163.1">
        <property type="protein sequence ID" value="ENSBIXP00000005233.1"/>
        <property type="gene ID" value="ENSBIXG00000011479.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039412.1">
        <property type="protein sequence ID" value="ENSBIXP00005024253.1"/>
        <property type="gene ID" value="ENSBIXG00005026856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016155"/>
    <dbReference type="OMA" id="LHFLCYV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061760">
        <property type="term" value="P:antifungal innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031665">
        <property type="term" value="P:negative regulation of lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032720">
        <property type="term" value="P:negative regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050729">
        <property type="term" value="P:positive regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.360.10">
        <property type="entry name" value="Antimicrobial Peptide, Beta-defensin 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025933">
        <property type="entry name" value="Beta_defensin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13841">
        <property type="entry name" value="Defensin_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="6" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6" id="KW-0211">Defensin</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516543" description="Beta-defensin" evidence="6">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-defensin" evidence="7">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU231113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="65" mass="7726" checksum="F6B81D12B5A30D53" modified="2019-09-18" version="1">MKIFYYLFHFLCYATFVLPALVDPERCSKLYGQCKKRCARYEKQIELCLSPSKICCIERAFEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H7T3</accession>
    <name>A0A4W2H7T3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA repair protein RAD51 homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RAD51 homolog B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">RAD51-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RAD51B</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair. Interacts with HELQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H7T3"/>
    <dbReference type="STRING" id="30522.A0A4W2H7T3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034708.1">
        <property type="protein sequence ID" value="ENSBIXP00000039842.1"/>
        <property type="gene ID" value="ENSBIXG00000006232.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015068.1">
        <property type="protein sequence ID" value="ENSBIXP00005028057.1"/>
        <property type="gene ID" value="ENSBIXG00005029614.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160169"/>
    <dbReference type="OMA" id="IHCQGHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001832">
        <property type="term" value="P:blastocyst growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061053">
        <property type="term" value="P:somite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19493">
        <property type="entry name" value="Rad51B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000806">
        <property type="entry name" value="DNA repair protein RAD51 homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058766">
        <property type="entry name" value="HHH_XRCC3_RAD51B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030548">
        <property type="entry name" value="RAD51B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46456">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46456:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26169">
        <property type="entry name" value="HHH_XRCC3_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="15">
        <location>
            <begin position="79"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="350" mass="38399" checksum="12ABFDD4067EB189" modified="2019-09-18" version="1">MGSKKLRRVGLSQELCDRLNRHQIVTCQDFLCLSPLELMKMTGLSYQGVHELLCLVSRACAPQMQTAYEIKTQRCAAHSSAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYFDTEEKLLLTSSKVHLYRELSCDEVLQRIESLEEEIISKGVKLVIIDSVASVVRKEFDTQLQGNVRERNKFLAREAASLKYLAEEFSIPVILTNQITTHLSGALASQADLVSPADDLSLSEGTSGSSCLTAALGNTWSHSVNTRLILQYLGSERRQILIAKSPLAPFTAFVYTIEKEGLVLQDQQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HBS1</accession>
    <name>A0A4W2HBS1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Regulator of G-protein signaling 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RGS1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with GNAI1 and GNAQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027420224.1">
        <property type="nucleotide sequence ID" value="XM_027564423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HBS1"/>
    <dbReference type="SMR" id="A0A4W2HBS1"/>
    <dbReference type="STRING" id="30522.A0A4W2HBS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019771.1">
        <property type="protein sequence ID" value="ENSBIXP00000033364.1"/>
        <property type="gene ID" value="ENSBIXG00000007384.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054415.1">
        <property type="protein sequence ID" value="ENSBIXP00005027643.1"/>
        <property type="gene ID" value="ENSBIXG00005030339.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906385"/>
    <dbReference type="CTD" id="5996"/>
    <dbReference type="GeneTree" id="ENSGT00940000157316"/>
    <dbReference type="OMA" id="CMVPHIE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061737">
        <property type="term" value="P:leukotriene signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of signal transduction"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGS"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF34">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="RGS" evidence="10">
        <location>
            <begin position="85"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23886" checksum="BE4CA788B4444BBB" modified="2019-09-18" version="1">MRAAAISTPRLDKMPGMFLSANPKELKGTDHSLLDDKTQKKRPKTFGMDVKAYLRSMIPHLESGMKSSKSKDLLSADEVKQWSESLEKLLANQTGQDVFGNFLKSEFSEENIEFWLACEDYKKTKSDLLHCKAEKIYKAFVQSDAAKQINIDFRTRESTAKKIKAPTPTSFDEAQKIIYTLMEKDSYPRFLKSNIYLNLLNDLQANSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HDZ1</accession>
    <name>A0A4W2HDZ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sarcalumenin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SRL</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Sarcoplasmic reticulum membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027383104.1">
        <property type="nucleotide sequence ID" value="XM_027527303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HDZ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053566.1">
        <property type="protein sequence ID" value="ENSBIXP00000026401.1"/>
        <property type="gene ID" value="ENSBIXG00000030096.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010959.1">
        <property type="protein sequence ID" value="ENSBIXP00005029390.1"/>
        <property type="gene ID" value="ENSBIXG00005006408.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883518"/>
    <dbReference type="CTD" id="6345"/>
    <dbReference type="GeneTree" id="ENSGT00940000157834"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000635">
        <property type="entry name" value="Sarcalumenin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051943">
        <property type="entry name" value="TRAFAC_Dynamin-like_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681:SF1">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611850" description="Sarcalumenin" evidence="8">
        <location>
            <begin position="21"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin-type G" evidence="9">
        <location>
            <begin position="90"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="473" mass="54458" checksum="D5428F20520F7EFE" modified="2019-09-18" version="1">MRALVLLCCFVASLLLPGQAEEAEDVSEEAPLRDRSHIEKTLMLNEDKPADDFSVVLQRLRKIYHSSIKPLEQSYKYNELRQHEITDGEITSKPMVLFLGPWSVGKSTMINYLLGLENTRYQLYTGAEPTTSEFTVLMHGPKLKTIEGIVMAADSARSFSPLEKFGQNFLEKLIGIEVPHKLLERVTFVDTPGIIENRKQQERGYPFNDVCQWFIDRADLIFVVFDPTKLDVGLELEMLFRQLKGRESQIRIILNKADNLATQMLMRVYGALFWSLAPLINVTEPPRVYVSSFWPYDYKPDTHRDLFLREEISLLEDLNQVIENRLENKIAFIRQHAIRVRIHALLVDRYLQTYKEKMTFFSDGELVFKDIVEDPDKFYIFKTILAKTNVSKFDLPHREAYKDFFGINPISSFKLLSQQCSYMGGCFLDKIERAITQELPSLLGSLGLGKNPGALNCDKTGCGETPKNRYKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HK94</accession>
    <name>A0A4W2HK94_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-transducin repeat containing E3 ubiquitin protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BTRC</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027385086.1">
        <property type="nucleotide sequence ID" value="XM_027529285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HK94"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004154.1">
        <property type="protein sequence ID" value="ENSBIXP00000030399.1"/>
        <property type="gene ID" value="ENSBIXG00000012371.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004586.1">
        <property type="protein sequence ID" value="ENSBIXP00005031490.1"/>
        <property type="gene ID" value="ENSBIXG00005011868.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884612"/>
    <dbReference type="CTD" id="8945"/>
    <dbReference type="GeneTree" id="ENSGT00940000159672"/>
    <dbReference type="OMA" id="GIAHVWS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22182">
        <property type="entry name" value="F-box_FBXW1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000001">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000004">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021977">
        <property type="entry name" value="Beta-TrCP_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF5">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12125">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01028">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="190"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="339"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="379"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="422"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="462"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="502"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="551"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="605" mass="68868" checksum="12F7ACF5F10B89C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HVR6</accession>
    <name>A0A4W2HVR6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Zinc finger E-box-binding homeobox 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Smad-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Zinc finger homeobox protein 1b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ZEB2</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. Represses expression of MEOX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HVR6"/>
    <dbReference type="STRING" id="30522.A0A4W2HVR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051480.1">
        <property type="protein sequence ID" value="ENSBIXP00000024242.1"/>
        <property type="gene ID" value="ENSBIXG00000026466.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021982.1">
        <property type="protein sequence ID" value="ENSBIXP00005035590.1"/>
        <property type="gene ID" value="ENSBIXG00005017016.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183208"/>
    <dbReference type="OMA" id="QENMFTP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048023">
        <property type="term" value="P:positive regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000013">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000082">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000105">
        <property type="entry name" value="Zinc finger E-box binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000117">
        <property type="entry name" value="Zinc finger E-box-binding homeobox 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF11">
        <property type="entry name" value="ZINC FINGER E-BOX-BINDING HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
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    <sequence length="1191" mass="134355" checksum="9518C515536208D1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="F:epidermal growth factor receptor binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activator activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050679">
        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045840">
        <property type="term" value="P:positive regulation of mitotic nuclear division"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="epigen isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="EPIGEN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSFORMING GROWTH FACTOR ALPHA"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612072" description="Epigen" evidence="15">
        <location>
            <begin position="24"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17001" checksum="4786238334FC80E7" modified="2019-09-18" version="1">MDFGVPISVYLLINAMTALTGEAASTISPPITTQQSNWTVNKTEADYTEGPIALKFSHPCLEDHHSYCINGVCAFHHELEKAICRCFTGYTGERCEHLTLTSYAADSYEKYIAIGIGVGLLLSGFLVIFYCYIRKRYLKLKSPYNICSGGRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IA58</accession>
    <name>A0A4W2IA58_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transmembrane p24 trafficking protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TMED9</name>
        <name evidence="12" type="synonym">LOC113895522</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2IA58"/>
    <dbReference type="SMR" id="A0A4W2IA58"/>
    <dbReference type="STRING" id="30522.A0A4W2IA58"/>
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        <property type="protein sequence ID" value="ENSBIXP00000021421.1"/>
        <property type="gene ID" value="ENSBIXG00000024513.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037245.1">
        <property type="protein sequence ID" value="ENSBIXP00005042249.1"/>
        <property type="gene ID" value="ENSBIXG00005007401.1"/>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="C:synaptic vesicle"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:syntaxin binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:Golgi organization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Emp24-like"/>
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        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="42"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
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    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="47"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="235" mass="27298" checksum="3F38C286A2021C16" modified="2019-09-18" version="1">MAAERSLWVVGLCPGSRLGRVVRVLLLLLWFAARGGALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRHLKSFFEAKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IKP4</accession>
    <name>A0A4W2IKP4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">POU3F1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027393568.1">
        <property type="nucleotide sequence ID" value="XM_027537767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IKP4"/>
    <dbReference type="SMR" id="A0A4W2IKP4"/>
    <dbReference type="STRING" id="30522.A0A4W2IKP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037265.1">
        <property type="protein sequence ID" value="ENSBIXP00000022223.1"/>
        <property type="gene ID" value="ENSBIXG00000025147.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042394.1">
        <property type="protein sequence ID" value="ENSBIXP00005044758.1"/>
        <property type="gene ID" value="ENSBIXG00005028613.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890107"/>
    <dbReference type="CTD" id="5453"/>
    <dbReference type="GeneTree" id="ENSGT00940000163458"/>
    <dbReference type="OMA" id="AHHGSWA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
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        <property type="term" value="P:myelination in peripheral nervous system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
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        <property type="entry name" value="POU_dom"/>
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        <property type="entry name" value="POU_domain_TF"/>
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        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF75">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_1"/>
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        <property type="entry name" value="HOMEOBOX_2"/>
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        <property type="entry name" value="POU_3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="13">
        <location>
            <begin position="247"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="337"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="339"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="69"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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            <end position="154"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="253"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="395"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
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            <begin position="220"/>
            <end position="232"/>
        </location>
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        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="RuleBase" id="RU361194"/>
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    <evidence type="ECO:0000256" key="11">
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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            <dbReference type="PROSITE" id="PS51179"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="451" mass="45496" checksum="9B33BD642C3992F1" modified="2019-09-18" version="1">MATTAQYLPRGPGGGAGGTGPLMHPDAAAAAAAAAAAERLHAGAAYREVQKLMHHEWLGAGAGHPVGLAHPQWLPTGGGGGGDWTGGPHLEHGKAGGGGTGRADDGGGGGGFHARLVHQGAAHAGAAWAQGGTAHHLGPAMSPSPGAGGGHQPQPLGLYAQAAYPGGGGGGLAGMLAAGGGGAGPGLHHALHEDGHEAQLEPSPPPHLGAHGHAHGHAHAGGLHAAAAHLHPGAGGGGSSVGEHSDEDAPSSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEETDSSSGSPTNLDKIAAQGRKRKKRTSIEVGVKGALESHFLKCPKPSAHEITGLADSLQLEKEVVRVWFCNRRQKEKRMTPAAGAGHPPMDDVYAPGELGPGGGSASPPSAPPPPPPAALHHHHHHTLPGSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ILH2</accession>
    <name>A0A4W2ILH2_BOBOX</name>
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        <submittedName>
            <fullName evidence="4">Sentan, cilia apical structure protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SNTN</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ILH2"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000000347.1">
        <property type="protein sequence ID" value="ENSBIXP00000000702.1"/>
        <property type="gene ID" value="ENSBIXG00000029480.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005055776.1">
        <property type="protein sequence ID" value="ENSBIXP00005046109.1"/>
        <property type="gene ID" value="ENSBIXG00005031067.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EH_dom"/>
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        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
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    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="122"/>
            <end position="170"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
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            <begin position="48"/>
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        </source>
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    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="170" mass="18757" checksum="38313F79FA867F1B" modified="2019-09-18" version="1">MTLATVAKQLLLRKSQHTTGDQSMCGCVHSTQDQALRLEGEPNPPAAPTSTLAPKNMPKSISISKQLASIKALRKGSDLEKAIATAALVFRNSSDPDGKLRKATAKNLLQTQFKNFAEGQETKARYKDLLSELDEHTENKLDFEDFMVLLLSVTIMSDLLQNIWSVKITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IMG5</accession>
    <name>A0A4W2IMG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 14">Mitochondrial import inner membrane translocase subunit TIM23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TIMM23B</name>
        <name evidence="15" type="synonym">LOC113885298</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has a role in the activation of stress-induced mitophagy by protecting PINK1 from OMA1-mediated degradation and facilitating its accumulation at the outer mitochondrial membrane in response to depolarization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM50. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15. Upon mitochondrial depolarization, interacts with PINK1; the interaction is required for PINK1 accumulation at the outer mitochondrial membrane, kinase activation by autophosphorylation and PRKN recruitement to mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2IMG5"/>
    <dbReference type="SMR" id="A0A4W2IMG5"/>
    <dbReference type="STRING" id="30522.A0A4W2IMG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048969.1">
        <property type="protein sequence ID" value="ENSBIXP00000039147.1"/>
        <property type="gene ID" value="ENSBIXG00000023083.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056248.1">
        <property type="protein sequence ID" value="ENSBIXP00005046494.1"/>
        <property type="gene ID" value="ENSBIXG00005031287.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001094"/>
    <dbReference type="OMA" id="DNDNIWS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005681">
        <property type="entry name" value="Tim23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00983">
        <property type="entry name" value="3a0801s02tim23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF39">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="209" mass="21827" checksum="C4F97A3D2FF44838" modified="2019-09-18" version="1">MEGGGGSGNKTTGGLAGFFGAGGAGYSHADLAGVPLTGMNPLSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLKETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCTGGLRGAARGGLAGLTLTGLYALYNNWEHMKGSVLQQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2INK2</accession>
    <name>A0A4W2INK2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Basic leucine zipper and W2 domains 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BZW1</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BZW family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419878.1">
        <property type="nucleotide sequence ID" value="XM_027564077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2INK2"/>
    <dbReference type="SMR" id="A0A4W2INK2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021422.1">
        <property type="protein sequence ID" value="ENSBIXP00000011848.1"/>
        <property type="gene ID" value="ENSBIXG00000017041.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056768.1">
        <property type="protein sequence ID" value="ENSBIXP00005046914.1"/>
        <property type="gene ID" value="ENSBIXG00005031551.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906122"/>
    <dbReference type="CTD" id="9689"/>
    <dbReference type="GeneTree" id="ENSGT00390000012561"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11560">
        <property type="entry name" value="W2_eIF5C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000006">
        <property type="entry name" value="Basic leucine zipper and W2 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051245">
        <property type="entry name" value="eIF5-mimic_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057397">
        <property type="entry name" value="HEAT_5MP1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043510">
        <property type="entry name" value="W2_5MP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14208">
        <property type="entry name" value="BASIC LEUCINE ZIPPER AND W2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14208:SF0">
        <property type="entry name" value="EIF5-MIMIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25504">
        <property type="entry name" value="HEAT_5MP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="247"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="419" mass="48043" checksum="9E952D52F5796F60" modified="2019-09-18" version="1">MNNQKQQKPTLSGQRFKTRKRDEKERFDPTQFQDCIIQGLTETGTDLEAVAKFLDASGAKLDYRRYAETLFDILVAGGMLAPGGTLADDMMRTDVCVFAAQEDLETMQAFAQVFNKLIRRYKYLEKGFEDEVKKLLLFLKGFSESERNKLAMLTGVLLANGTLNASILNSLYNENLVKEGVSAAFAVKLFKSWINEKDINAVAASLRKVSMDNRLMELFPANKQSVEHFTKYFTEAGLKELSEYVRNQQTIGARKELQKELQEQMSRGDPFKDIILYVKEEMKKNNIPEPVVIGIVWSSVMSTVEWNKKEELVAEQAIKHLKQYSPLLAAFTTQGQSELTLLLKIQEYCYDNIHFMKAFQKIVVLFYKAEVLSEEPILKWYKDAHVAKGKSVFLEQMKKFVEWLKNAEEESESEAEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BL12</accession>
    <name>A0A4W2BL12_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transducin beta like 1 X-linked</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat EBI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BL12"/>
    <dbReference type="STRING" id="30522.A0A4W2BL12"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052961.1">
        <property type="protein sequence ID" value="ENSBIXP00000000529.1"/>
        <property type="gene ID" value="ENSBIXG00000029722.1"/>
    </dbReference>
    <dbReference type="OMA" id="KWNKCGN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000001">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000014">
        <property type="entry name" value="Putative F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF52">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN TBL1X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="570" mass="61368" checksum="5DC0CAD053612C96" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
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        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024470.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="98" mass="11503" checksum="83B3B1BC23E81C56" modified="2019-09-18" version="1">MGRHFLLLLFLLGCPLAVTPLRCITCHLRTQTDRCRRGFGVCVAKNYESCMILKIFQGGTLQLSYLVCQRFCRDLTYSFQGRIYVHKCCNYNYCNFKR</sequence>
</entry>
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    <accession>A0A4W2BNF1</accession>
    <name>A0A4W2BNF1_BOBOX</name>
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    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_027525987.1"/>
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        <property type="nucleotide sequence ID" value="XM_027525990.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027525991.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027525992.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cerebellin 4"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
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        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF50">
        <property type="entry name" value="CEREBELLIN-2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COMPLEMNTC1Q"/>
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        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <location>
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        </location>
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        <source>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001509.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="224" mass="23901" checksum="767D4CED3EDF1A11" modified="2019-09-18" version="1">MLAPGRGPRGPPLTMPGRRGALREPAGCGSGLGAALALLLLLLPAGCPVRAQNDTEPIVLEGKCLVVCDSSPSADGAVTSSLGISVRSGSAKVAFSATRSTNHEPSEMSNRTMTIYFDQVLVNIGNHFDLASSIFVAPRKGIYSFSFHVVKVYNRQTIQVSLMQNGYPVISAFAGDQDVTREAASNGVLLLMEREDKVHLKLERGNLMGGWKYSTFSGFLVFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTE9</accession>
    <name>A0A4W2BTE9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ALS2 C-terminal-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ALS2CL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase. Regulates the ALS2-mediated endosome dynamics.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Forms a heteromeric complex with ALS2. Interacts with ALS2 and RAB5A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTE9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010036.1">
        <property type="protein sequence ID" value="ENSBIXP00000003345.1"/>
        <property type="gene ID" value="ENSBIXG00000009754.1"/>
    </dbReference>
    <dbReference type="OMA" id="HWQEGSM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.80:FF:000006">
        <property type="entry name" value="ALS2 C-terminal-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051984">
        <property type="entry name" value="Alsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057248">
        <property type="entry name" value="Alsin-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089:SF1">
        <property type="entry name" value="ALS2 C-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089">
        <property type="entry name" value="ALSIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25383">
        <property type="entry name" value="PH_alsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VPS9" evidence="8">
        <location>
            <begin position="796"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="953" mass="107844" checksum="4765DFF5B23BA3E1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUV5</accession>
    <name>A0A4W2BUV5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUV5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011204.1">
        <property type="protein sequence ID" value="ENSBIXP00000002778.1"/>
        <property type="gene ID" value="ENSBIXG00000009233.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="570" mass="65614" checksum="76969B6F3D83D2C7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZU2</accession>
    <name>A0A4W2BZU2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZU2"/>
    <dbReference type="STRING" id="30522.A0A4W2BZU2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004668.1">
        <property type="protein sequence ID" value="ENSBIXP00000005969.1"/>
        <property type="gene ID" value="ENSBIXG00000012121.1"/>
    </dbReference>
    <dbReference type="OMA" id="VIRYNTR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000066">
        <property type="entry name" value="H2B histone family member W testis-specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="59"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="165" mass="18437" checksum="BC999C490F700626" modified="2019-09-18" version="1">MENGGSVVSQPSPDGYEEDVITKETGTSETEPSETEMAKAETSKPEPCDAEPKKAKRKTAKGRRHRRRCHQDNFASFATYFRRVLKQVHTGLSLSHEAMNVMHSFVKHMFEQIAEEAGSLAHSSKHCTITSGEIQRAVRLLLPGEIGKHAVSEATKSVIRYNTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2S8</accession>
    <name>A0A4W2C2S8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tryptase</fullName>
            <ecNumber evidence="5">3.4.21.59</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.</text>
            <dbReference type="EC" id="3.4.21.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.59" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C2S8"/>
    <dbReference type="SMR" id="A0A4W2C2S8"/>
    <dbReference type="STRING" id="30522.A0A4W2C2S8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004484.1">
        <property type="protein sequence ID" value="ENSBIXP00000006069.1"/>
        <property type="gene ID" value="ENSBIXG00000012214.1"/>
    </dbReference>
    <dbReference type="OMA" id="PRTWNTF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF162">
        <property type="entry name" value="SERINE PROTEASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="40"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00050838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="320" mass="35186" checksum="049B6A4DFE8973FF" modified="2019-09-18" version="1">MDGSLEMMRRRGSEGQDKRTFTFLSPLPLVCGRPVYSGRVVGGKDAAATRWPWQVSLQLSNSHVCGGSLLSDRWIVTAAHCLQMTRIPFLYTVQLGSVERDNLDEGVILRVSQIVIHPTYSNVSGDIALLRLFSRVTYSSSILPICLSNVKKKRLIIPDSCWVTGWGKLKEEDADYPTILQEAEVPIIKRQKCENIYNPLGSFLPQIQPVIMETMICAGDVKKGKDTCQGDSGGPLACHIDGIWILIGLSSWGRGCGISFPGIYTNVTYYQKWIMSVISRAEVLGANNLNLSDFLFSIVPLSLALLGPSCVFGSNILPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C4H0</accession>
    <name>A0A4W2C4H0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-dependent RNA helicase TDRD9</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tudor domain-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">TDRD9</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with piRNA-associated proteins PIWIL1 and PIWIL4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="STRING" id="30522.A0A4W2C4H0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001396.1">
        <property type="protein sequence ID" value="ENSBIXP00000007646.1"/>
        <property type="gene ID" value="ENSBIXG00000013588.1"/>
    </dbReference>
    <dbReference type="OMA" id="QRSAYCS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20431">
        <property type="entry name" value="Tudor_TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000073">
        <property type="entry name" value="ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001113">
        <property type="entry name" value="ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000012">
        <property type="entry name" value="putative ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000946">
        <property type="entry name" value="putative ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000016">
        <property type="entry name" value="Tudor domain containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047384">
        <property type="entry name" value="Tudor_TDRD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF113">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="12" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="172"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="23">
        <location>
            <begin position="404"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="21">
        <location>
            <begin position="974"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="1412" mass="159017" checksum="1BB430C6F80A823E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <location>
            <begin position="198"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007009.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="383" mass="44761" checksum="9F476BD428D55934" modified="2019-09-18" version="1">MEVLDIVQETRIKTIPMEKKCKKAKWLSEEALQIAVKRRQAKSKGEKERYKHLNAEFQRIARRDKKAFFSDQCKEIEENNRMGKTRDLFKKIRDTKGIFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVFTHLEPDILECEVKRALGSITTNKASGGDGIPVELFQILKDDAEKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYMNREVPDVQAGFRKGRGTRDQIANIRWIIEIAREFQKNIYFCFIDYTKAFDSVDHNKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTIDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAHDVLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5I8</accession>
    <name>A0A4W2C5I8_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6M7</accession>
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            <fullName evidence="4">6-pyruvoyltetrahydropterin synthase</fullName>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <name>Zn(2+)</name>
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        </cofactor>
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    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.</text>
    </comment>
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        <text evidence="3">Belongs to the PTPS family.</text>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="135" mass="15126" checksum="5D957575F42BAE4C" modified="2019-09-18" version="1">MSSHEWPGLGSSPMGTSVPPRLFGTTHHPHSKSLSDEEDLKLFGKCHNTHTFKVPGEIGPVTGMVLNLTDLQEYTEEAIMKYLGLDVPYFADIVSTTENVDIYTWENLQKFLPVGVLDKIKVYETNNNIVVYKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8F3</accession>
    <name>A0A4W2C8F3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA carboxylase 1</fullName>
            <ecNumber evidence="4">6.4.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">ACC-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">ACACA</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:11309"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027373930.1">
        <property type="nucleotide sequence ID" value="XM_027518129.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A4W2C8F3"/>
    <dbReference type="STRING" id="30522.A0A4W2C8F3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011643.1">
        <property type="protein sequence ID" value="ENSBIXP00000002573.1"/>
        <property type="gene ID" value="ENSBIXG00000007094.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031938.1">
        <property type="protein sequence ID" value="ENSBIXP00005039959.1"/>
        <property type="gene ID" value="ENSBIXG00005023249.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877728"/>
    <dbReference type="CTD" id="31"/>
    <dbReference type="GeneTree" id="ENSGT00940000156706"/>
    <dbReference type="OMA" id="PTPKGHC"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071380">
        <property type="term" value="P:cellular response to prostaglandin E stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001894">
        <property type="term" value="P:tissue homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF5">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0092">Biotin</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="26" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610397" description="Acetyl-CoA carboxylase 1" evidence="26">
        <location>
            <begin position="22"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin carboxylation" evidence="29">
        <location>
            <begin position="154"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="312"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="27">
        <location>
            <begin position="782"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="30">
        <location>
            <begin position="1613"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="31">
        <location>
            <begin position="1955"/>
            <end position="2271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00045132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00075764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
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    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="2383" mass="269848" checksum="B850C20E008CDF70" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8Q4</accession>
    <name>A0A4W2C8Q4_BOBOX</name>
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        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000002676.1"/>
        <property type="gene ID" value="ENSBIXG00000009141.1"/>
    </dbReference>
    <dbReference type="OMA" id="KHREVHV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000032">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000355">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000258">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29 isoform X2"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
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        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
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        <property type="entry name" value="KRAB"/>
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    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
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        <property type="entry name" value="Myb_DNA-bind_4"/>
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        <property type="entry name" value="SCAN_dom"/>
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        <property type="entry name" value="SCAN_sf"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
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    </dbReference>
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        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="19">
        <location>
            <begin position="47"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="20">
        <location>
            <begin position="231"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="777"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="805"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="833"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="861"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="889"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="917"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="162"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="589"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="950"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="622"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="958"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="969" mass="110592" checksum="0422B4652A39B7CA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBW4</accession>
    <name>A0A4W2CBW4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glial fibrillary acidic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SYNM.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBW4"/>
    <dbReference type="STRING" id="30522.A0A4W2CBW4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019186.1">
        <property type="protein sequence ID" value="ENSBIXP00000010395.1"/>
        <property type="gene ID" value="ENSBIXG00000002418.1"/>
    </dbReference>
    <dbReference type="OMA" id="QYETMAT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904714">
        <property type="term" value="P:regulation of chaperone-mediated autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF9">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04732">
        <property type="entry name" value="Filament_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="10">
        <location>
            <begin position="65"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="69"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="271"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="468" mass="53765" checksum="66BD3F6CF6AC42CB" modified="2019-09-18" version="1">MERRRVTSATRRSYVSSSEMVVGGRRLGPGTRLSLARMPPPLPARVDFSLAGALNSGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLGTLRQKLQDETNQRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVASSNMHEAEEWYRSKFADLNDAAARNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHAREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRGVKSTKEGESHKVTRHLKSLTIQVIPIQAHQVVDGAPPALETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCQ9</accession>
    <name>A0A4W2CCQ9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TATA-box binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCQ9"/>
    <dbReference type="STRING" id="30522.A0A4W2CCQ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019679.1">
        <property type="protein sequence ID" value="ENSBIXP00000010710.1"/>
        <property type="gene ID" value="ENSBIXG00000002474.1"/>
    </dbReference>
    <dbReference type="OMA" id="HMMPMSE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33548" checksum="E206865DAA9E2F42" modified="2019-09-18" version="1">MDRNDSLSPFDQYLASPQGAMSPGIPVFSPVMHYGTGLTPQPIQSTDSLSTSEEEQQQQTTVRTGRLSTTSQEATQEALDQTPQLFDSQPLTTAPLLGTTPLYPSPMTPMIPMTPAMPASEIAELVPQLHNVVCTVNLGCKLDLKSIALLARNAEYNPKRFAAVIMRIREPRTTALLFSSGKMVCTGGKTEEQSRLAARKYARIIQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEPLVLSHQQFASYEPELFPGLVYRMIKPRIVLLIFVSGKVVLTGAKVRSEIYEAFNNIYSILKGFRKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEM3</accession>
    <name>A0A4W2CEM3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL19m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L19, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEM3"/>
    <dbReference type="STRING" id="30522.A0A4W2CEM3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045088.1">
        <property type="protein sequence ID" value="ENSBIXP00000011429.1"/>
        <property type="gene ID" value="ENSBIXG00000016665.1"/>
    </dbReference>
    <dbReference type="OMA" id="IHEIQVV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.790:FF:000002">
        <property type="entry name" value="39S ribosomal protein L19, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="287" mass="32846" checksum="69D50AE5246E039F" modified="2019-09-18" version="1">MAPASSPNITAFGPCRLPSDLPVSTSVASRVLAGPGRRQITGPSEPGVFQPPPKPVIVDKRGPQRRESRFLSPEFIPPRGRTNPLKFQIERKDMLERRKILHIPEFYVGSILRVTTADPYANGKTSQFLGICIQRSGKGLGATFILRNTIEGQGVEICFELYNPRIQEIQVVKLEKRLDDSLLYLRDALPEYSTFDMNMKPVAQEPSREVPINQLKVKMKPKPWSKRWERPKFNVKGIRFDLCLTEEQMKEAQKWSQPWLEFDMMREYDTSKIEAAIWNEIEASKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHP4</accession>
    <name>A0A4W2CHP4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SIX homeobox 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SIX3</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SIX/Sine oculis homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022532.1">
        <property type="protein sequence ID" value="ENSBIXP00000012557.1"/>
        <property type="gene ID" value="ENSBIXG00000006588.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031434.1">
        <property type="protein sequence ID" value="ENSBIXP00005018872.1"/>
        <property type="gene ID" value="ENSBIXG00005003768.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160346"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000046">
        <property type="entry name" value="SIX homeobox 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF31">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="221"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="223"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="249"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="368" mass="40128" checksum="71D0B3D8F8B68255" modified="2019-09-18" version="1">MNIYPPLLPAPGFPYLSTGQSMVFRSPLDLYSSHFLLPNFADSHHRSLLLASSGGGNGSGGGGGGSGGNGVGGGGAGGAGGGGGGGSRAPPEELSMFQLPTLNFSPEQVASVCETLEETGDIERLGRFLWSLPVAPGACEAINKHESILRARAVVAFHTGNFRDLYHILENHKFTKESHGKLQAMWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRSLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQHQAIGPSGMRSLAEPGCPTHGSAESPSTAASPTTSVSSLTERADTGTSILSDKHRFSFRFPRTRHPTCMTIYLYLGKYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK52</accession>
    <name>A0A4W2CK52_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PTPN7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CK52"/>
    <dbReference type="SMR" id="A0A4W2CK52"/>
    <dbReference type="STRING" id="30522.A0A4W2CK52"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003267.1">
        <property type="protein sequence ID" value="ENSBIXP00000006686.1"/>
        <property type="gene ID" value="ENSBIXG00000012776.1"/>
    </dbReference>
    <dbReference type="OMA" id="GYDGREK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14612">
        <property type="entry name" value="PTPc-N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000020">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008356">
        <property type="entry name" value="Tyr_Pase_KIM-con"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46198">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46198:SF3">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01778">
        <property type="entry name" value="KIMPTPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="76"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="244"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="270"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR608356-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR608356-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="339" mass="38293" checksum="09341B18338CE867" modified="2019-09-18" version="1">MTQPPPDQAPAKKHVRLQERRGSNVALMLDVRSLGAVEPICSVRTPQEVTLHFLRTAGRPLSRQALQHRPPSPTQLEEEFLRIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGQDKAYIATQGPMPNTVSDFWEMVWQEEAPLIIMLTQLREGKEKCVHYWPTEEETYGPFRIRVQDVRERPEYTVRTLTIQHQEQRRALKHILFSAWPDHQTPESAGPLLRLVAEVEDGPETGARSGPIVVHCSAGIGRTGCFIATRIGCQQLKARGEVDILSIVCQLRLDRGGMIQTAEQYQFLHHTLALFAAQLPEEPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CN35</accession>
    <name>A0A4W2CN35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC113904663</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CN35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023782.1">
        <property type="protein sequence ID" value="ENSBIXP00000013377.1"/>
        <property type="gene ID" value="ENSBIXG00000018316.1"/>
    </dbReference>
    <dbReference type="OMA" id="VSYCSAN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="43"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="317" mass="35439" checksum="9B573EAD1540690F" modified="2019-09-18" version="1">MAPGNRNHTHVTEFILMGVSDHPELQIPFFCVFLIIYGLTLAGNLGIIILTSLDSQLQTPMYFFLKHLAIINLGNSSVIAPKMLVNFLVTKKTISYYGCAIQLGGFLVFIVTEIFVLAAMACDRYVAICSPLLYQVVVSPRICLLLAALIYIYSLTTALTVSSCVFSMSYCSSNAINHFYCDNVPLLALSCSDTYIPETAVFTFSGTNLFFSMIIVLISYFNIILAILRIRSSEGRQKAFSTCASHMMAVTVFYGTLLFMYLQPRTNHSLDTDKMASVFYTLVIPMLNPFIYSLRNKDVKDALKRFLDNPCQSFKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSC8</accession>
    <name>A0A4W2CSC8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-catenin-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CTNNBIP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Prevents the interaction between CTNNB1 and TCF family members, and acts as a negative regulator of the Wnt signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds CTNNB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTNNBIP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027420761.1">
        <property type="nucleotide sequence ID" value="XM_027564960.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027420762.1">
        <property type="nucleotide sequence ID" value="XM_027564961.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027420763.1">
        <property type="nucleotide sequence ID" value="XM_027564962.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027420764.1">
        <property type="nucleotide sequence ID" value="XM_027564963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSC8"/>
    <dbReference type="SMR" id="A0A4W2CSC8"/>
    <dbReference type="STRING" id="30522.A0A4W2CSC8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026023.1">
        <property type="protein sequence ID" value="ENSBIXP00000014870.1"/>
        <property type="gene ID" value="ENSBIXG00000000547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906599"/>
    <dbReference type="CTD" id="56998"/>
    <dbReference type="OMA" id="MNCEGAS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.490:FF:000001">
        <property type="entry name" value="beta-catenin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.490">
        <property type="entry name" value="Beta-catenin-interacting ICAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009428">
        <property type="entry name" value="ICAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036911">
        <property type="entry name" value="ICAT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47142">
        <property type="entry name" value="BETA-CATENIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47142:SF1">
        <property type="entry name" value="BETA-CATENIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06384">
        <property type="entry name" value="ICAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81730">
        <property type="entry name" value="beta-catenin-interacting protein ICAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Beta-catenin-interacting ICAT" evidence="11">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="81" mass="9228" checksum="C586DE90B517636A" modified="2019-09-18" version="1">MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTANEEEFLRTYAGVVNSQLSQLPQHSIDQGAEDVVMAFSRSETEDRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CST7</accession>
    <name>A0A4W2CST7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Retinol dehydrogenase 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CST7"/>
    <dbReference type="SMR" id="A0A4W2CST7"/>
    <dbReference type="STRING" id="30522.A0A4W2CST7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026227.1">
        <property type="protein sequence ID" value="ENSBIXP00000015029.1"/>
        <property type="gene ID" value="ENSBIXG00000003505.1"/>
    </dbReference>
    <dbReference type="OMA" id="FRWYQER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000074">
        <property type="entry name" value="Retinol dehydrogenase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF11">
        <property type="entry name" value="RETINOL DEHYDROGENASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35669" checksum="442E1473282BA0ED" modified="2019-09-18" version="1">MWLYLVVLVGLYYLLRWYRERQVVSHLRDKFVFITGCDSGFGNLLARQLDLRGLRVLAACLTEKGAEQLRNQTSDRLQTVILDVTKTDSVAAATEWVKERVGDRGLWGLVNNAGISTPTAPNEWLTKQDFMKILDVNLLGVIDVTLSVLPLVRKARGRVVNVSSIMGRVALLGGGYCMSKYGVEAFSDSLRRELSYFGVKVVMIEPGYFKTAVTSPKALSQGFQASWNQASPEIKELYGEKFMANLMKSVDLLETTSCQDLSLVTNCMEHALTACHPRTRYSPGWDAKFIYLPMSYLPSFLVDLMVYWRNPRPAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUT9</accession>
    <name>A0A4W2CUT9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein zwilch</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RZZ complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Chromosome</location>
            <location evidence="1 9">Centromere</location>
            <location evidence="1 9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZWILCH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUT9"/>
    <dbReference type="STRING" id="30522.A0A4W2CUT9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018684.1">
        <property type="protein sequence ID" value="ENSBIXP00000010061.1"/>
        <property type="gene ID" value="ENSBIXG00000015637.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.230:FF:000001">
        <property type="entry name" value="protein zwilch homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018630">
        <property type="entry name" value="Zwilch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995:SF1">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09817">
        <property type="entry name" value="Zwilch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="277" mass="30686" checksum="06B6D9A6789A7F6B" modified="2019-09-18" version="1">KGIRKDPFIYEADIQVQLISKGQPNPLKNILHENETIFIVEKVPLEKEEPSPIEELQSEDTAISDLSTGENVGLLALPIGRARQLIGFYTMAHNPNMTHLKINRPVTALPPLWVRCDGSDPEGISWLGAELISTSSNITGIVLYMVTCKVDKNYSVNLEDLKKSHKKRHHLSTLTASGFAQYELFKSTALDDTVAASQTTITLDISWSPVDEILQTPPLSSTATLNIKVESGEPRGPLSQLHRELKFLLGHTLLASWAESCFCSPTTNPRPLPYTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVT8</accession>
    <name>A0A4W2CVT8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tudor domain containing 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TDRD15</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2CVT8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030100.1">
        <property type="protein sequence ID" value="ENSBIXP00000017528.1"/>
        <property type="gene ID" value="ENSBIXG00000021532.1"/>
    </dbReference>
    <dbReference type="OMA" id="NMSFECL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20436">
        <property type="entry name" value="Tudor_TDRD15_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20437">
        <property type="entry name" value="Tudor_TDRD15_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047450">
        <property type="entry name" value="Tudor_TDRD15_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047452">
        <property type="entry name" value="Tudor_TDRD15_rpt2"/>
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    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF7">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tudor/PWWP/MBT"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="59"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="289"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="531"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="797"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="1784"/>
            <end position="1842"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2123" mass="242647" checksum="F13667D3B5573B78" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXP4</accession>
    <name>A0A4W2CXP4_BOBOX</name>
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        <recommendedName>
            <fullName evidence="9">STAGA complex 65 subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
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        <alternativeName>
            <fullName evidence="10">Suppressor of Ty 7-like</fullName>
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        <name evidence="14" type="primary">SUPT7L</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subunit">
        <text evidence="8">Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, SUPT7L, GCN5L2, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12 and TAF9.</text>
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            <location evidence="1">Nucleus</location>
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        <source>
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        </source>
    </evidence>
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        <source>
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    <sequence length="412" mass="46118" checksum="81CD2535FA7EA294" modified="2019-09-18" version="1">MLRYWGEIPISSSQTNRSSFDLLPREFRLVEVHDPPLHQPSANKPKPPTMLDIPSEPCSLTIHTIQLIQHNRRLRNLIATAQAQNQQQTEGVKTEETEPLPSCPGSPPLPDDLLPLDCKNPSAPFQVWHSDPESDFYRGKGEPVTELSWHSCRQLLYQAVATILAHTGFECANESVLETLTDVAHEYCLKFTKLLRFAVDRESRLGQTPFPDVMEQVFHEVGIGSVLSLQKFWQHRIKDYHSYMLQISKQLSEEYERIVNPEKATEDTKPVKIKEEPVSDITFPVSEELEADLASGDQSLPMGVLGTQTERFPSNLEVEASPQASSTEVNASPLWNLAHVKMEPQESEEGNVSGHGVLGSDVFEEPMSGMSEAGIPQSPDDSDSSYGSHSTDSLMGSSPVFNQRCKKRMRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D0F0</accession>
    <name>A0A4W2D0F0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="12">Regulator of G-protein signaling 3</fullName>
        </recommendedName>
    </protein>
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        <name evidence="16" type="primary">RGS3</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D0F0"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RGS"/>
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        <property type="entry name" value="RGS_RGS3"/>
    </dbReference>
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        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
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        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46848">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46848:SF1">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PDZ" evidence="14">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="15">
        <location>
            <begin position="786"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="388"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="718"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="448"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="596"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="911" mass="100234" checksum="27CECEC906FCC816" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2G0</accession>
    <name>A0A4W2D2G0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine decarboxylase antizyme 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OAZ1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome. Triggers ODC degradation by inducing the exposure of a cryptic proteasome-interacting surface of ODC. Stabilizes AZIN2 by interfering with its ubiquitination. Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Involved in the translocation of AZIN2 from ER-Golgi intermediate compartment (ERGIC) to the cytosol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with ODC1 and thereby sterically blocks ODC homodimerization. Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with AZIN2; this interaction disrupts the interaction between the antizyme and ODC1. Interacts with FAM171A1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2G0"/>
    <dbReference type="STRING" id="30522.A0A4W2D2G0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033845.1">
        <property type="protein sequence ID" value="ENSBIXP00000019977.1"/>
        <property type="gene ID" value="ENSBIXG00000000082.1"/>
    </dbReference>
    <dbReference type="OMA" id="HVDHVFI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000001">
        <property type="entry name" value="Ornithine decarboxylase antizyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF8">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01337">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0688">Ribosomal frameshifting</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="225" mass="24428" checksum="35B1BEACBCDA2086" modified="2019-09-18" version="1">NPPCSGSSTATASPERRKGINPAPPSTPPAPCRSLACTAAEAAAVRVPGSPSTAVVTWVQGLGGAPDVPHPPLKIPGGRGNSQRDHNLSANLFYSDNRLNVTEELTSNNKTRIFNVQSRLTEAKHINWRAVLSNTCLYVEIPGGALPEGSKDSFAVLLEFAEEQLHVDHVFICFHKNRDDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMAYTFERESSGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3P1</accession>
    <name>A0A4W2D3P1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">40S ribosomal protein S20</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 40S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3P1"/>
    <dbReference type="SMR" id="A0A4W2D3P1"/>
    <dbReference type="STRING" id="30522.A0A4W2D3P1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034520.1">
        <property type="protein sequence ID" value="ENSBIXP00000020442.1"/>
        <property type="gene ID" value="ENSBIXG00000004732.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043854.1">
        <property type="protein sequence ID" value="ENSBIXP00005027138.1"/>
        <property type="gene ID" value="ENSBIXG00005001752.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003248"/>
    <dbReference type="OMA" id="TMAMGMI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="10">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="119" mass="13329" checksum="93F88D66D17CFBC7" modified="2019-09-18" version="1">MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCVDLIGGVKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEVVKQITSITIEPGVKVEVTIADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4B1</accession>
    <name>A0A4W2D4B1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Mitochondrial adenyl nucleotide antiporter SLC25A23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Short calcium-binding mitochondrial carrier protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Solute carrier family 25 member 23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">SLC25A23</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Electroneutral antiporter that mediates the transport of adenine nucleotides through the inner mitochondrial membrane. Originally identified as an ATP-magnesium/inorganic phosphate antiporter, it also acts as a broad specificity adenyl nucleotide antiporter. By regulating the mitochondrial matrix adenine nucleotide pool could adapt to changing cellular energetic demands and indirectly regulate adenine nucleotide-dependent metabolic pathways. Also acts as a regulator of mitochondrial calcium uptake and can probably transport trace amounts of other divalent metal cations in complex with ATP. In vitro, a low activity is also observed with guanyl and pyrimidine nucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3'-AMP(in) + ADP(out) + H(+)(out) = 3'-AMP(out) + ADP(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73679"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:60880"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3'-AMP(out) + phosphate(in) = 3'-AMP(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73691"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ADP(out) + diphosphate(in) = ADP(in) + diphosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73671"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ADP(out) + phosphate(in) + H(+)(out) = ADP(in) + phosphate(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>AMP(out) + phosphate(in) = AMP(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:70259"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Mg(2+)(in) + ADP(out) + ATP(in) + H(+)(out) = Mg(2+)(out) + ADP(in) + ATP(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73659"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dADP(in) + ADP(out) = dADP(out) + ADP(in)</text>
            <dbReference type="Rhea" id="RHEA:72855"/>
            <dbReference type="ChEBI" id="CHEBI:57667"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>dADP(out) + phosphate(in) + H(+)(out) = dADP(in) + phosphate(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57667"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dAMP(in) + ADP(out) + H(+)(out) = dAMP(out) + ADP(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58245"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>dAMP(out) + phosphate(in) = dAMP(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73687"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58245"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>phosphate(in) + ATP(out) + 2 H(+)(out) = phosphate(out) + ATP(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72035"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with MCU. Interacts with MICU1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 30">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4B1"/>
    <dbReference type="STRING" id="30522.A0A4W2D4B1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032439.1">
        <property type="protein sequence ID" value="ENSBIXP00000019035.1"/>
        <property type="gene ID" value="ENSBIXG00000022740.1"/>
    </dbReference>
    <dbReference type="OMA" id="TYEWYKR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051503">
        <property type="term" value="P:adenine nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000190">
        <property type="entry name" value="calcium-binding mitochondrial carrier protein SCaMC-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000028">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000003">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 29 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 30" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="77"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="29">
        <location>
            <begin position="183"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="29">
        <location>
            <begin position="277"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="482"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00055026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00065306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00070247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00079652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="536" mass="59944" checksum="FEC5D2BDF385EDD0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5U4</accession>
    <name>A0A4W2D5U4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lysophosphatidylserine lipase ABHD12</fullName>
            <ecNumber evidence="4">3.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-arachidonoylglycerol hydrolase ABHD12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Abhydrolase domain-containing protein 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Monoacylglycerol lipase ABHD12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Oxidized phosphatidylserine lipase ABHD12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes. Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system. Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes glycerol monoesters of long-chain fatty acids.</text>
            <dbReference type="EC" id="3.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine esterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.23" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5U4"/>
    <dbReference type="STRING" id="30522.A0A4W2D5U4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024812.1">
        <property type="protein sequence ID" value="ENSBIXP00000014086.1"/>
        <property type="gene ID" value="ENSBIXG00000018872.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052651">
        <property type="term" value="P:monoacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000069">
        <property type="entry name" value="monoacylglycerol lipase ABHD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF61">
        <property type="entry name" value="LYSOPHOSPHATIDYLSERINE LIPASE ABHD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="20">
        <location>
            <begin position="256"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="531"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="557" mass="61593" checksum="98B6B63500E834CC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8A3</accession>
    <name>A0A4W2D8A3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8A3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037214.1">
        <property type="protein sequence ID" value="ENSBIXP00000022197.1"/>
        <property type="gene ID" value="ENSBIXG00000025115.1"/>
    </dbReference>
    <dbReference type="OMA" id="HIWPWNT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="352"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="676" mass="78648" checksum="944264FDB176D114" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8N7</accession>
    <name>A0A4W2D8N7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="15">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CDC25B</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. The three isoforms seem to have a different level of activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with MAPK14 and 14-3-3 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8N7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026378.1">
        <property type="protein sequence ID" value="ENSBIXP00000015121.1"/>
        <property type="gene ID" value="ENSBIXG00000019702.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000006">
        <property type="entry name" value="M-phase inducer phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF48">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06617">
        <property type="entry name" value="M-inducer_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 15" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 15" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="17">
        <location>
            <begin position="384"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="533" mass="59983" checksum="B32CF5BDE8D07AA3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D962</accession>
    <name>A0A4W2D962_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cysteine rich hydrophobic domain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CHIC2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027400319.1">
        <property type="nucleotide sequence ID" value="XM_027544518.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2D962"/>
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        <property type="protein sequence ID" value="ENSBIXP00000022539.1"/>
        <property type="gene ID" value="ENSBIXG00000025354.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010287.1">
        <property type="protein sequence ID" value="ENSBIXP00005003454.1"/>
        <property type="gene ID" value="ENSBIXG00005009594.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894291"/>
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    <dbReference type="GeneTree" id="ENSGT00390000003601"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:Golgi-associated vesicle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CHIC1/2"/>
    </dbReference>
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        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
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        <property type="entry name" value="CYSTEINE-RICH HYDROPHOBIC DOMAIN PROTEIN BRAIN X-LINKED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13005:SF3">
        <property type="entry name" value="CYSTEINE-RICH HYDROPHOBIC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="4">
        <location>
            <begin position="45"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="165" mass="19254" checksum="DC32A9C58524B55E" modified="2019-09-18" version="1">MADFDEIYEEEEDEERALEEQLLKYSPDPVVVRGSGHVTVFGLSNKFESEFPSSLTGKVAPEEFKASINRVNSCLKKNLPVNVRWLLCGCLCCCCTLGCSMWPVICLSKRTRRSIEKLLEWENNRLYHKLCLHWRLSKRKCETNNMMEYVILIEFLPKTPIFRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9S8</accession>
    <name>A0A4W2D9S8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras-related GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9S8"/>
    <dbReference type="STRING" id="30522.A0A4W2D9S8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035459.1">
        <property type="protein sequence ID" value="ENSBIXP00000021050.1"/>
        <property type="gene ID" value="ENSBIXG00000024225.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000002">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000276">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF7">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="346" mass="40283" checksum="ECC7261B3D48BBA4" modified="2019-09-18" version="1">RTDSPLWLHQYTEARPSRLSPRNPRLLPPSVCAAVRPRPEKKVLLMGKSRSGKTSMRSIIFANYIACDTRRLGATINVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNAPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLGNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRLETISNIIQQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKRSLLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA51</accession>
    <name>A0A4W2DA51_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heterogeneous nuclear ribonucleoprotein A3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element, A2RE. May be involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Identified in the spliceosome C complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA51"/>
    <dbReference type="SMR" id="A0A4W2DA51"/>
    <dbReference type="STRING" id="30522.A0A4W2DA51"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038200.1">
        <property type="protein sequence ID" value="ENSBIXP00000022859.1"/>
        <property type="gene ID" value="ENSBIXG00000025638.1"/>
    </dbReference>
    <dbReference type="OMA" id="PRNSWDT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12763">
        <property type="entry name" value="RRM1_hnRNPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12582">
        <property type="entry name" value="RRM2_hnRNPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000158">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein A3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000350">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein A3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034516">
        <property type="entry name" value="hnRNPA1/3_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026:SF27">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A3-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026">
        <property type="entry name" value="HOMOLOGOUS TO DROSOPHILA SQD (SQUID) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="104"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="357" mass="36837" checksum="E3CC10C08D6D2ECC" modified="2019-09-18" version="1">MEGHDPKEAEQLRKLFIGGLSFETTDDSLREHFEKWGTLTDCVVMRDPQTKCSRGFGFVTYSCVEEVDAAMCARPHKVDGRVVEPKRAVSREDSVKPGAHLTVKKIFVGGIKEDTDEYNLRDYFEKYGKIETIEVMEDRQSGKKRGFAFVTFDDHDTVDKIVVQKYHTINGHNCEVKKALSKQEMQSAGSQRGRGGGSGNFMGRGGNSGGGGGNFGHGGNFGGRGGCGGGGGGSRGSYGGGDGGYNGFGGDGGNYGSGPGYSSRGANGAGGPGGHGNQGGGYGGGGGGYDGYNEGGNFGGNYGGGGNYNDFGNYSGQQQSNYGPMKGGSFGGRSSGSPYGGGYGSGGGSGGYGSRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAG8</accession>
    <name>A0A4W2DAG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAG8"/>
    <dbReference type="SMR" id="A0A4W2DAG8"/>
    <dbReference type="STRING" id="30522.A0A4W2DAG8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047145.1">
        <property type="protein sequence ID" value="ENSBIXP00000015743.1"/>
        <property type="gene ID" value="ENSBIXG00000000457.1"/>
    </dbReference>
    <dbReference type="OMA" id="IVNYEKQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048020">
        <property type="term" value="F:CCR chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070098">
        <property type="term" value="P:chemokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00271">
        <property type="entry name" value="Chemokine_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000023">
        <property type="entry name" value="Lymphotactin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008105">
        <property type="entry name" value="Chemokine_XCL1/XCL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF101">
        <property type="entry name" value="CYTOKINE SCM-1 BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01731">
        <property type="entry name" value="LYMPHOTACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021383014" description="Chemokine interleukin-8-like domain-containing protein" evidence="8">
        <location>
            <begin position="16"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="9">
        <location>
            <begin position="28"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="97" mass="10609" checksum="BCF8D47E7F301B9A" modified="2019-09-18" version="1">MKLLILTCLVICSLAAYTVEGVGSEVLEKSICVSLTTQRLPIKNIKTYTIKEGSVKAVIFITRRGFKICADPQAAWVKKAVQKIDRKNISQAKPTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIA7</accession>
    <name>A0A4W2DIA7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Farnesyl pyrophosphate synthase</fullName>
            <ecNumber evidence="7">2.5.1.1</ecNumber>
            <ecNumber evidence="6">2.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="30">Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>isopentenyl diphosphate + (2E)-geranyl diphosphate = (2E,6E)-farnesyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19361"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>isopentenyl diphosphate + dimethylallyl diphosphate = (2E)-geranyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22408"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 31">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="7"/>
    <dbReference type="EC" id="2.5.1.10" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIA7"/>
    <dbReference type="SMR" id="A0A4W2DIA7"/>
    <dbReference type="STRING" id="30522.A0A4W2DIA7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051307.1">
        <property type="protein sequence ID" value="ENSBIXP00000024031.1"/>
        <property type="gene ID" value="ENSBIXG00000001543.1"/>
    </dbReference>
    <dbReference type="OMA" id="CSWVVNQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000052">
        <property type="entry name" value="Farnesyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="21" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="17" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="11 31" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00053104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="421" mass="48317" checksum="783EB116E6BEE313" modified="2019-09-18" version="1">MLPLSRWLRSVGVFLLPAPCWVPQDRLLGPLQRPTLGHGCPVLGAWHSARCWRQAWTEEPRAFYSSLRMNGDQKLDAYAQERQDFIQHFSQIVKVLTEEDIGHPEIGDAITRLKEVLEYNAIGGKYNRGLTVVITFRELVEPGKQDPDSLQRALTVGWCVELLQAFFLVSDDIMDSSLTRRGQTCWYQKPGIGLDAINDAFLLESSIYRLLKLYCREQPYYLDLIELFLQSSYQTEIGQTLDLITAPQGNVDLGRFTEKRYKSIVKYKTAFYSFYLPVAAAMYMAGIDGEKEHAHAKKILLEMGEFFQIQDDYLDLFGDPSMTGKIGTDIQDNKCSWLVVQCLQRASPEQRQILQENYGQKEAEKVARVKALYEEMNLSAVYMQYEEDSYNHIMGLIEQYAAPLPPAIFLGLAQKIYKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJ64</accession>
    <name>A0A4W2DJ64_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Centaurin-delta-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ARAP3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SAM domain) with INPPL1/SHIP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027401947.1">
        <property type="nucleotide sequence ID" value="XM_027546146.1"/>
    </dbReference>
    <dbReference type="STRING" id="30522.A0A4W2DJ64"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040140.1">
        <property type="protein sequence ID" value="ENSBIXP00000024167.1"/>
        <property type="gene ID" value="ENSBIXG00000026414.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021024.1">
        <property type="protein sequence ID" value="ENSBIXP00005011910.1"/>
        <property type="gene ID" value="ENSBIXG00005007324.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895230"/>
    <dbReference type="CTD" id="64411"/>
    <dbReference type="GeneTree" id="ENSGT00940000158869"/>
    <dbReference type="OMA" id="TLNTMEM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
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        <source>
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    <sequence length="1548" mass="170668" checksum="CA71FA4E33D9EAFD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Kazal-like" evidence="4">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="105" mass="11245" checksum="B0EE8713C28421EF" modified="2019-09-18" version="1">MADAVVVTILSNLCITAVSIMKTTGGLLLLCVVAHLCSCSEAASLPLKTVNCSIYKKYPVVAIPCPITYLPVCGSDYITYGNECHLCIENLKSYGKVQFLHEGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM04</accession>
    <name>A0A4W2DM04_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Troponin I, cardiac muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cardiac troponin I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Troponin I, fast skeletal muscle</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Troponin I, fast-twitch isoform</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TNNI2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Binds to actin and tropomyosin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388232.1">
        <property type="nucleotide sequence ID" value="XM_027532431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DM04"/>
    <dbReference type="STRING" id="30522.A0A4W2DM04"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013589.1">
        <property type="protein sequence ID" value="ENSBIXP00000027355.1"/>
        <property type="gene ID" value="ENSBIXG00000008165.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038106.1">
        <property type="protein sequence ID" value="ENSBIXP00005023330.1"/>
        <property type="gene ID" value="ENSBIXG00005026138.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886321"/>
    <dbReference type="CTD" id="7136"/>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="OMA" id="KRHRAIT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031014">
        <property type="term" value="F:troponin T binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000002">
        <property type="entry name" value="troponin I, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF15">
        <property type="entry name" value="TROPONIN I, FAST SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="21404" checksum="E5D0BD3037893B50" modified="2019-09-18" version="1">MGDEEKRHRAITARRQHLKSVMLQIAATELEKEEGRREAEKQNYLSEHCPPLHLPGSMSEVQELCRQLHAKIDAAEEEKYDMEIRVQKSTKELEDMNQKLFDLRGKFKRPPLRRVRMSADAMLKALLGSKHKVCMDLRANLKQVKKEDTEKERDLRDVGDWRKNIEEKSGMEGRKKMFETES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM27</accession>
    <name>A0A4W2DM27_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DM27"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013482.1">
        <property type="protein sequence ID" value="ENSBIXP00000027385.1"/>
        <property type="gene ID" value="ENSBIXG00000008217.1"/>
    </dbReference>
    <dbReference type="OMA" id="PWFWWVE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="183" mass="20974" checksum="6A9F78D9698132C8" modified="2025-10-08" version="2">MDWEQQTGTQQLDWEQPHEPQPPLEPPQEPQPPLEPPQEPQPPLEPPQEPRSPHMSHSPPWSPHTSRSPLCHSWSRSRLAHSSIRACSSRRAHSSWSHSPHSWSRSPHSWSHSLRNSQSSPWFWWVEGGVGQRSRWRERRCSVWGLLGRSLYIPAGAGVTWARAPFLVPVWAAFVSSLPLCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQL4</accession>
    <name>A0A4W2DQL4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Histone-lysine N-methyltransferase SETD2</fullName>
            <ecNumber evidence="3">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Lysine N-methyltransferase 3A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Protein-lysine N-methyltransferase SETD2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">SETD2</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="38 39" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="3"/>
    <dbReference type="STRING" id="30522.A0A4W2DQL4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009858.1">
        <property type="protein sequence ID" value="ENSBIXP00000028581.1"/>
        <property type="gene ID" value="ENSBIXG00000009850.1"/>
    </dbReference>
    <dbReference type="OMA" id="RTCFPME"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="SMR" id="A0A4W2DQT8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035988.1">
        <property type="protein sequence ID" value="ENSBIXP00000021348.1"/>
        <property type="gene ID" value="ENSBIXG00000004940.1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0035556">
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        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000431">
        <property type="term" value="P:regulation of cytokinesis, actomyosin contractile ring assembly"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="BRCT_Ect2_rpt1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="protein ECT2 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.10190:FF:000015">
        <property type="entry name" value="protein ECT2 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="protein ECT2 isoform X2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="protein ECT2 isoform X3"/>
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        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Ect2"/>
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        <property type="entry name" value="ECT2_BRCT0"/>
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    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="25" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
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    </feature>
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    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00075521"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="914" mass="103589" checksum="A89156DBCE00906F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DST9</accession>
    <name>A0A4W2DST9_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">DET1- and DDB1-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDA1 family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000029643.1"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033575">
        <property type="entry name" value="DDA1-like"/>
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    <dbReference type="InterPro" id="IPR018276">
        <property type="entry name" value="DDA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879:SF2">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10172">
        <property type="entry name" value="DDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DET1- and DDB1-associated protein 1" evidence="5">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="116" mass="13536" checksum="4A68FE915208A638" modified="2019-09-18" version="1">MGSIKDRNGIDLTEAEDIKKRWQEYTEDNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELAGESSAPPRKVARTDSPDMHEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT12</accession>
    <name>A0A4W2DT12_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT12"/>
    <dbReference type="STRING" id="30522.A0A4W2DT12"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013582.1">
        <property type="protein sequence ID" value="ENSBIXP00000027356.1"/>
        <property type="gene ID" value="ENSBIXG00000008163.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054042.1">
        <property type="protein sequence ID" value="ENSBIXP00005045391.1"/>
        <property type="gene ID" value="ENSBIXG00005029103.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222806"/>
    <dbReference type="OMA" id="RFSPARC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610924" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="53" mass="5653" checksum="2F7A090781E76153" modified="2025-10-08" version="2">MGGRIQPTPCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DU41</accession>
    <name>A0A4W2DU41_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SRPK1</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
            <location evidence="13">Nuclear body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DU41"/>
    <dbReference type="STRING" id="30522.A0A4W2DU41"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005058.1">
        <property type="protein sequence ID" value="ENSBIXP00000030114.1"/>
        <property type="gene ID" value="ENSBIXG00000011923.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005495.1">
        <property type="protein sequence ID" value="ENSBIXP00005005875.1"/>
        <property type="gene ID" value="ENSBIXG00005011520.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155264"/>
    <dbReference type="OMA" id="HEGQRPP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000895">
        <property type="entry name" value="SRSF protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000163">
        <property type="entry name" value="SRSF protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF4">
        <property type="entry name" value="SRSF PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="80"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="238"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="391"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="686" mass="78054" checksum="18E0CCA8E90B2DF7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWH7</accession>
    <name>A0A4W2DWH7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SKA complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Spindle and kinetochore-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SKA1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Component of the SKA complex, composed of SKA1, SKA2 and SKA3. The SKA complex is a homodimer organized around a central W-shaped coiled-coil structure, formed by the interacting domains of SKA1, SKA2, and SKA3, each end of the 'W' is extended further by the C-terminal microtubule-binding domains of SKA1 and SKA3; the complex forms extended structures on microtubules. Interacts (via SXLP motif) with MAPRE1 (via C-terminus); the interaction is direct and stabilizes the kinetochore-microtubule attachment of the SKA1 complex. Interacts (via C-terminus) with protein phosphatase PP1 subunit PPP1CA; the interaction is direct and required for recruitment of PPP1CA to the kinetochore. Interacts with the NDC80 complex; the interaction is required to establish kinetochore-microtubule end-on attachments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SKA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWH7"/>
    <dbReference type="SMR" id="A0A4W2DWH7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009080.1">
        <property type="protein sequence ID" value="ENSBIXP00000028861.1"/>
        <property type="gene ID" value="ENSBIXG00000010225.1"/>
    </dbReference>
    <dbReference type="OMA" id="PTGMRED"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051656">
        <property type="term" value="P:establishment of organelle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12958">
        <property type="entry name" value="SKA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1890:FF:000001">
        <property type="entry name" value="Spindle and kinetochore-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1890">
        <property type="entry name" value="Ska1 microtubule binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009829">
        <property type="entry name" value="SKA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042031">
        <property type="entry name" value="SKA1_MBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28573">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28573:SF1">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07160">
        <property type="entry name" value="SKA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="254" mass="29428" checksum="9A3F72AA8E84B53B" modified="2019-09-18" version="1">MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWJ4</accession>
    <name>A0A4W2DWJ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorting nexin-15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SNX15</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in several stages of intracellular trafficking. Overexpression of SNX15 disrupts the normal trafficking of proteins from the plasma membrane to recycling endosomes or the TGN.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027387832.1">
        <property type="nucleotide sequence ID" value="XM_027532031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWJ4"/>
    <dbReference type="SMR" id="A0A4W2DWJ4"/>
    <dbReference type="STRING" id="30522.A0A4W2DWJ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010444.1">
        <property type="protein sequence ID" value="ENSBIXP00000028391.1"/>
        <property type="gene ID" value="ENSBIXG00000009542.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886105"/>
    <dbReference type="CTD" id="29907"/>
    <dbReference type="OMA" id="EMLVDQH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02677">
        <property type="entry name" value="MIT_SNX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000017">
        <property type="entry name" value="sorting nexin-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000029">
        <property type="entry name" value="sorting nexin-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051866">
        <property type="entry name" value="Intracell_Sig-Traffick_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508:SF9">
        <property type="entry name" value="SORTING NEXIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="226"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="345" mass="38403" checksum="56C0009501B6BE25" modified="2019-09-18" version="1">MSRQAKDDFLRHYTVSDPRTHPKGYTEYKVTAQFISKRDPEDVKEVVVWKRYSDFRKLHGDLAYTHRNLFRRLEEFPAFPRGQVFGRFEASVIEERRKGAEDLLRFTVHIPALNNSPQLKEFFRGGEVTRPSEVSGDLHILPPPLIPTPPPDEPRVQPHETWLPQPLPAERRGLEELEVPADPPPSSPAQEALDLLFNCGSTEEASSSPARGPLTEAELALFDPFSKEEGAGPSPTHIGELAALEAGSGRPDQEPWEPGGQAEEDDEEGEPAPAYLSQATELITQALRDEKAGAYPAALQGYRDGVHILLQGVPGDPSPARREGVKKKAAEYLKRAEEILHLHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXV9</accession>
    <name>A0A4W2DXV9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CXXC-type zinc finger protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CpG-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PHD finger and CXXC domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CXXC1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXV9"/>
    <dbReference type="STRING" id="30522.A0A4W2DXV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009090.1">
        <property type="protein sequence ID" value="ENSBIXP00000028831.1"/>
        <property type="gene ID" value="ENSBIXG00000010216.1"/>
    </dbReference>
    <dbReference type="OMA" id="IRVGHKP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15553">
        <property type="entry name" value="PHD_Cfp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000138">
        <property type="entry name" value="CXXC-type zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022056">
        <property type="entry name" value="CpG-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF1">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12269">
        <property type="entry name" value="CpG_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="18">
        <location>
            <begin position="159"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="84"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="218"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="324"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="655" mass="75485" checksum="C2C1A83DCA3FEC3B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0L6</accession>
    <name>A0A4W2E0L6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF163">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="81" mass="9270" checksum="892948E3C71C15AF" modified="2019-09-18" version="1">MAASQGWLTFQDIAIDFTQEEWECLDLGQRELYRDTMLENYGNLASLGLVSKPDLVTFLERMKDSRNISSMETTPIYPGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4E8</accession>
    <name>A0A4W2E4E8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Condensin complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">NCAPD2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG. Interacts with histones H1 and H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2E4E8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000498.1">
        <property type="protein sequence ID" value="ENSBIXP00000031610.1"/>
        <property type="gene ID" value="ENSBIXG00000013933.1"/>
    </dbReference>
    <dbReference type="OMA" id="CPLEKLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000695">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 16" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 16" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="10 16" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="19">
        <location>
            <begin position="77"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="18">
        <location>
            <begin position="1092"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="980"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1325"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="980"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1390"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR017127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1422" mass="159012" checksum="D019423CBE9180F4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5P8</accession>
    <name>A0A4W2E5P8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113886531</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5P8"/>
    <dbReference type="STRING" id="30522.A0A4W2E5P8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014743.1">
        <property type="protein sequence ID" value="ENSBIXP00000027031.1"/>
        <property type="gene ID" value="ENSBIXG00000028934.1"/>
    </dbReference>
    <dbReference type="OMA" id="KRSYCEQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="308" mass="34459" checksum="C46CC3AFF6F84D8C" modified="2019-09-18" version="1">MATENQSTVTEFILGGLTNRPEFQFPLFFLFLGIYLVTMVGNLGMITLICLNPQLHTPMYYFLSNLSFVDLCYSSVITPKMLVNFVSGKNTISYAGCMAQLYFFQVFVIAECYMLTVMAYDRYVAICRPLLYNIIMSHRVCSLLVVGVYAMGLIGSTIETGLMLKLSYCDFLISHYFCEILTLMTLSCSSTYDIEMTTFFLAGFNIVVTSLTVHVSYAFIISSILHITSTEGRSKAFSTCSSHLAAVGLFYGSSSFMYLKPSTASSLAQENVASMFYTTVIPMLNPLIYSLRNKEVRAAIQKTLRVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7U1</accession>
    <name>A0A4W2E7U1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thyroid hormone receptor interactor 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TRIP11</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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    <name>A0A4W2EEH8_BOBOX</name>
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        <submittedName>
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        </submittedName>
    </protein>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13447:SF2">
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="183" mass="20214" checksum="069B9A1E8E880263" modified="2019-09-18" version="1">MAALCRTRAVTAESHFLRVFLFSRSCRGAGTESGSGSESSNSTEPRPRPGGFASALERHSELQRKAELGSPKNVESFASMLRHSPLTQMGPAKDKIVIGRIFHIVENDLYIDFGGKFHCVCKRPEVDGEKYQKGTRVRLRLLDLELTSRFLGATTDTTILEAEAVLLGLQESKDSKSKEERGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFI8</accession>
    <name>A0A4W2EFI8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFI8"/>
    <dbReference type="STRING" id="30522.A0A4W2EFI8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023286.1">
        <property type="protein sequence ID" value="ENSBIXP00000034921.1"/>
        <property type="gene ID" value="ENSBIXG00000003092.1"/>
    </dbReference>
    <dbReference type="OMA" id="SARDECP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12382">
        <property type="entry name" value="RRM_RBMX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000119">
        <property type="entry name" value="RNA-binding motif protein, X chromosome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012604">
        <property type="entry name" value="RBM1CTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08081">
        <property type="entry name" value="RBM1CTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="241"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="396" mass="42373" checksum="46F715FE1343F0DD" modified="2019-09-18" version="1">MVEADRPGKLFIGGLNLETDEKSLEATFGKYGRISEVLLMKDRETNKSRGFAFITFESPADAKAAVRDMNGKSLDGKAIKVAQATKPAFESGRRGPPLSRSRGRSRGLRGARGGGPRRPPSRGGPADDGGYAGDFDLRPSRAPLPMKRGPPPPRRAGPPPKRAAPSGPARSGSGGGMRGRAPAARGRDGYEGPPRRDPPPPRRDPYLGSREGGYSPRDGYSSRDYSSARDARDFAPSPREYTYRDYGHSSARDECPSRGYGDRDGYGGRDRDYADHPSGGSYRDTFESYGDPRSAAPARGPPPSYGGGGGRYEEYRGCSPDAYGGGRDGYAGGRSERYSGGRDRVGRADRGLPQSVERGCPPPRESYSRSGRKVPRGGGRLGSRSERGGGGGRSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFJ4</accession>
    <name>A0A4W2EFJ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubby-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFJ4"/>
    <dbReference type="STRING" id="30522.A0A4W2EFJ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023300.1">
        <property type="protein sequence ID" value="ENSBIXP00000034931.1"/>
        <property type="gene ID" value="ENSBIXG00000018047.1"/>
    </dbReference>
    <dbReference type="OMA" id="HNKTPSW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000001">
        <property type="entry name" value="Tubby-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005398">
        <property type="entry name" value="Tubby_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF24">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16322">
        <property type="entry name" value="Tub_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01574">
        <property type="entry name" value="TUBBYPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Tubby N-terminal" evidence="9">
        <location>
            <begin position="27"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby C-terminal" evidence="8">
        <location>
            <begin position="275"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="524" mass="58176" checksum="AB9AAEE62802CD2C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGV0</accession>
    <name>A0A4W2EGV0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear apoptosis inducing factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NAIF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027412188.1">
        <property type="nucleotide sequence ID" value="XM_027556387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGV0"/>
    <dbReference type="STRING" id="30522.A0A4W2EGV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027461.1">
        <property type="protein sequence ID" value="ENSBIXP00000036717.1"/>
        <property type="gene ID" value="ENSBIXG00000003683.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045493.1">
        <property type="protein sequence ID" value="ENSBIXP00005011073.1"/>
        <property type="gene ID" value="ENSBIXG00005002340.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901687"/>
    <dbReference type="CTD" id="203245"/>
    <dbReference type="GeneTree" id="ENSGT00450000040324"/>
    <dbReference type="OMA" id="QQDGMIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902108">
        <property type="term" value="P:regulation of mitochondrial membrane permeability involved in apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098">
        <property type="entry name" value="AGAP001331-PA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098:SF7">
        <property type="entry name" value="NUCLEAR APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="327" mass="35194" checksum="E876D72EF24D28F8" modified="2019-09-18" version="1">MAVPAKKRKMNFSEREVEIIVEELELKKHLLVNHFNAGVPLAAKSAAWHGILRRVNAVATCRRELPEVKKKWSDLKTEVRRKVAQVRAAVEGGEAPGPTEEDGAGGPGTGGGSGAGGPAVAPVLLTPMQQRICNLLGEATIISLPSTAEIHPVALGPTATAAAATVTLTQIPTETTYHTLEEGVVEYCTAEAPPPLPAEAPVEMMAQHADTSVKPQALKSRIALNSAKLIQEQRVTNLHVKEIAQHLEQQNDLLQMIRRSQEVQACAQERQAQAMEGTQAALSVLIQVLRPMIKDFRRYLQSNMPNPATASEPGQVAQNGQPDSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIZ7</accession>
    <name>A0A4W2EIZ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">40S ribosomal protein S20</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 40S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EIZ7"/>
    <dbReference type="SMR" id="A0A4W2EIZ7"/>
    <dbReference type="STRING" id="30522.A0A4W2EIZ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028212.1">
        <property type="protein sequence ID" value="ENSBIXP00000037032.1"/>
        <property type="gene ID" value="ENSBIXG00000020551.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047152.1">
        <property type="protein sequence ID" value="ENSBIXP00005014431.1"/>
        <property type="gene ID" value="ENSBIXG00005018414.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003248"/>
    <dbReference type="OMA" id="AIHHIRM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="10">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="95" mass="10671" checksum="CD849CADEE03616D" modified="2019-09-18" version="1">MAFKDTGKTPVEPEVAIHRIRITLTSPNVKSLEKVWADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCVKSNSCSYMKSLLLSCCFIWSSAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKP5</accession>
    <name>A0A4W2EKP5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Leucine rich repeat neuronal 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LRRN3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027395352.1">
        <property type="nucleotide sequence ID" value="XM_027539551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKP5"/>
    <dbReference type="SMR" id="A0A4W2EKP5"/>
    <dbReference type="STRING" id="30522.A0A4W2EKP5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046083.1">
        <property type="protein sequence ID" value="ENSBIXP00000035224.1"/>
        <property type="gene ID" value="ENSBIXG00000018470.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007449.1">
        <property type="protein sequence ID" value="ENSBIXP00005004887.1"/>
        <property type="gene ID" value="ENSBIXG00005010699.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891472"/>
    <dbReference type="CTD" id="54674"/>
    <dbReference type="GeneTree" id="ENSGT00940000160513"/>
    <dbReference type="OMA" id="DKFYMHP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000355">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000481">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000056">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000074">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000090">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
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        <property type="entry name" value="FN3"/>
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    </dbReference>
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        <property type="entry name" value="LRR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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    <feature type="chain" id="PRO_5044611142" evidence="12">
        <location>
            <begin position="23"/>
            <end position="708"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="628"/>
            <end position="649"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="421"/>
            <end position="514"/>
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        <location>
            <begin position="523"/>
            <end position="614"/>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="708" mass="79072" checksum="CD2EE11785A9309F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ENF6</accession>
    <name>A0A4W2ENF6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="20">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="20">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 27">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer; homodimerizes following heparin- and ligand-binding. Interacts with CBL, IRS1, PIK3R1 and PLCG1. Interacts with FRS2 and SHC1. Interacts with PTN and MDK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05036">
        <property type="entry name" value="PTKc_ALK_LTK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000154">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
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    </dbReference>
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        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALK/LTK-like_GRD"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF276">
        <property type="entry name" value="ALK TYROSINE KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12810">
        <property type="entry name" value="ALK_LTK_GRD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021321540" description="Tyrosine-protein kinase receptor" evidence="22">
        <location>
            <begin position="22"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="24">
        <location>
            <begin position="270"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="24">
        <location>
            <begin position="484"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="1122"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1436"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1476"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1592"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1445"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1487"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1546"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="1156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1621" mass="176176" checksum="4F5E79045EA533A1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ENI0</accession>
    <name>A0A4W2ENI0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Angiogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ANG</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the pancreatic ribonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027409215.1">
        <property type="nucleotide sequence ID" value="XM_027553414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ENI0"/>
    <dbReference type="SMR" id="A0A4W2ENI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048359.1">
        <property type="protein sequence ID" value="ENSBIXP00000038299.1"/>
        <property type="gene ID" value="ENSBIXG00000022102.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027062.1">
        <property type="protein sequence ID" value="ENSBIXP00005015967.1"/>
        <property type="gene ID" value="ENSBIXG00005003224.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900006"/>
    <dbReference type="CTD" id="283"/>
    <dbReference type="GeneTree" id="ENSGT00940000162981"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019731">
        <property type="term" value="P:antibacterial humoral response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06265">
        <property type="entry name" value="RNase_A_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.130.10:FF:000001">
        <property type="entry name" value="Ribonuclease pancreatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001427">
        <property type="entry name" value="RNaseA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023411">
        <property type="entry name" value="RNaseA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023412">
        <property type="entry name" value="RNaseA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437:SF60">
        <property type="entry name" value="ANGIOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00074">
        <property type="entry name" value="RnaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00794">
        <property type="entry name" value="RIBONUCLEASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00092">
        <property type="entry name" value="RNAse_Pc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54076">
        <property type="entry name" value="RNase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00127">
        <property type="entry name" value="RNASE_PANCREATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ribonuclease A-domain" evidence="12">
        <location>
            <begin position="26"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="148" mass="17004" checksum="B794FC4104DD81DD" modified="2019-09-18" version="1">MVMVLSPLFLVFILGLGLTPVAPAQDDYRYIHFLTQHYDAKPKGRNDEYCFNMMKNRRLTRPCKDRNTFIHGNKNDIKAICEDRNGQPYRGDLRISKSEFQITICKHKGGSSRPPCRYGATEDSRVIVVGCENGLPVHFDESFITPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQ04</accession>
    <name>A0A4W2EQ04_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Solute carrier family 51 member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SLC51A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027393020.1">
        <property type="nucleotide sequence ID" value="XM_027537219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EQ04"/>
    <dbReference type="SMR" id="A0A4W2EQ04"/>
    <dbReference type="STRING" id="30522.A0A4W2EQ04"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032719.1">
        <property type="protein sequence ID" value="ENSBIXP00000038972.1"/>
        <property type="gene ID" value="ENSBIXG00000004079.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043643.1">
        <property type="protein sequence ID" value="ENSBIXP00005045429.1"/>
        <property type="gene ID" value="ENSBIXG00005029116.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889865"/>
    <dbReference type="CTD" id="200931"/>
    <dbReference type="GeneTree" id="ENSGT00940000160780"/>
    <dbReference type="OMA" id="CLPMVIP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015125">
        <property type="term" value="F:bile acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032782">
        <property type="term" value="P:bile acid secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="340" mass="37858" checksum="C9EB139D53E909B9" modified="2019-09-18" version="1">MEPDRTQIRLDPRYTADLLEILKTNYSVPSACFSYPPTAAQLLRALGPVDISLMVIMTLFVLGSIAIFLEAAVYLHKNTRCPIKRKTLIWCSSSPTIVSAFSCFGLWIPRALTLVEMAITTFYSMCFYLLMQAMVEGFGGKEAVLRTLKDTPVMIHTGPCCCCCPCCPRIKITRKRLQLLLLGPIQYAFFKISLTLVGLFLIPDGIFDPSDISEGSTALWINTFLGVSTLSALWTIGIIFRQARLHLGEQNIGAKFVLFQALLILSALQPSIFSVLASGGQIACSPPFSSKIRSQVMNCHLLILESFLITVLTRIYYRRKDDKLGYEPFSSPDQDLNLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESG3</accession>
    <name>A0A4W2ESG3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESG3"/>
    <dbReference type="STRING" id="30522.A0A4W2ESG3"/>
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        <property type="protein sequence ID" value="ENSBIXP00000039718.1"/>
        <property type="gene ID" value="ENSBIXG00000023658.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:sensory perception of smell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TryX_like_family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RdCVF2"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46762:SF1">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin_8"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021449703" description="Thioredoxin-like fold domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="3">
        <location>
            <begin position="335"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="465" mass="51031" checksum="28BB6FDCEA8B3285" modified="2019-09-18" version="1">MKNTVLTIFSFATFILVLSFKKCQGPVHARFIVLVVPLNLFLPSISPTNPPFFQEDSESELWQHASWNSINPRREFPLALALPSAGTLSARAQYFGTNLEKEQGPGLPSAGSLPPTDRTALTFPRWWDPWDRWQGIERRVGRAPRGLRARARREGPRGERGGGGLARVAEKSASPRSAEAASSEQRRTQSCAGRWDVQRVAEERVGRGAEAGGGGAEAEGGGAGAGRLGSLEAGAAVVGATAATPGVAGVCECFPLLQRGRACSRRRPRLRLVPRSGHPVVRREPDIATRPQVSFCRRCPHPQVSPRPDMVDVLGGRRLVTCDGAWVEAEAALQNKVVALYFAAGRCAPSRDFTPLLCDFYEELVDEARPPAPFEVVFVSADGSAQEMLEFMKELHGAWLALPFHDPYRHELRTRYHITAIPRLVILKPSGEVITDKGRKQIRERGLACFQNWVEAADIFQNFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETD6</accession>
    <name>A0A4W2ETD6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory inhibitor subunit 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PPP1R1C</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411910.1">
        <property type="nucleotide sequence ID" value="XM_027556109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ETD6"/>
    <dbReference type="STRING" id="30522.A0A4W2ETD6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035805.1">
        <property type="protein sequence ID" value="ENSBIXP00000040297.1"/>
        <property type="gene ID" value="ENSBIXG00000024340.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013313.1">
        <property type="protein sequence ID" value="ENSBIXP00005028621.1"/>
        <property type="gene ID" value="ENSBIXG00005006079.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901579"/>
    <dbReference type="CTD" id="151242"/>
    <dbReference type="GeneTree" id="ENSGT00940000160192"/>
    <dbReference type="OMA" id="VNEREDQ"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PPP1R1A/B/C"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15417">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR AND DOPAMINE- AND CAMP-REGULATED NEURONAL PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DARPP-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="109" mass="12341" checksum="5463529436C919DE" modified="2019-09-18" version="1">MEPNSPKKIQFAVPLFQSQIAPEAAEQIRKRRPTPASLVILNEHNPPEIDDKRVTNTQAEAQNATPKQRKQSVYTAPAMKGVKHMKGQNESAFPEEEEGVNERAQQWDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETN6</accession>
    <name>A0A4W2ETN6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cadherin-related family member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">CDHR2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Intermicrovillar adhesion molecule that forms, via its extracellular domain, calcium-dependent heterophilic complexes with CDHR5 on adjacent microvilli. Thereby, controls the packing of microvilli at the apical membrane of epithelial cells. Through its cytoplasmic domain, interacts with microvillus cytoplasmic proteins to form the intermicrovillar adhesion complex/IMAC. This complex plays a central role in microvilli and epithelial brush border differentiation. May also play a role in cell-cell adhesion and contact inhibition in epithelial cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5. Interacts with MAST2. Interacts (via cytoplasmic domain) with USH1C and MYO7B; required for proper localization of CDHR2 to microvilli tips and its function in brush border differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Cell projection</location>
            <location evidence="16">Microvillus membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2ETN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035650.1">
        <property type="protein sequence ID" value="ENSBIXP00000040268.1"/>
        <property type="gene ID" value="ENSBIXG00000024304.1"/>
    </dbReference>
    <dbReference type="OMA" id="QTVMLVQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000221">
        <property type="entry name" value="Cadherin related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000245">
        <property type="entry name" value="Cadherin related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000252">
        <property type="entry name" value="Cadherin related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000168">
        <property type="entry name" value="Cadherin-related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000264">
        <property type="entry name" value="Cadherin-related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000101">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000094">
        <property type="entry name" value="protocadherin gamma-C4 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF133">
        <property type="entry name" value="CADHERIN-RELATED FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021406195" description="Cadherin-related family member 2" evidence="22">
        <location>
            <begin position="22"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1158"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="127"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="244"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="371"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="484"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="589"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="699"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="813"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="933"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1264"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1279"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1311" mass="142267" checksum="A02F32F918CBC892" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV52</accession>
    <name>A0A4W2EV52_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Sucrase-isomaltase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SI</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EV52"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037093.1">
        <property type="protein sequence ID" value="ENSBIXP00000040891.1"/>
        <property type="gene ID" value="ENSBIXG00000007991.1"/>
    </dbReference>
    <dbReference type="OMA" id="AQHVWIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000005">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000016">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.110.10:FF:000003">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017957">
        <property type="entry name" value="P_trefoil_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF66">
        <property type="entry name" value="SUCRASE-ISOMALTASE, INTESTINAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00025">
        <property type="entry name" value="P_TREFOIL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0765">Sulfation</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="47"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="913"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1812" mass="208520" checksum="E094ABD39E844AE3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZZ6</accession>
    <name>A0A4W2EZZ6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BICD cargo adaptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">BICD1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3P0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042489.1">
        <property type="protein sequence ID" value="ENSBIXP00000043632.1"/>
        <property type="gene ID" value="ENSBIXG00000027782.1"/>
    </dbReference>
    <dbReference type="OMA" id="RWTELLY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="437" mass="49552" checksum="13EDE2CF5D9B28C6" modified="2019-09-18" version="1">MGKTRDLFKKIRDTKGTFHAKMGSIKDSNGMDLTEAEDIKKKWQEYTEELYKKDLHDKDNHDGVITHSPKARHPCEVKWALESITMNEGSGGDGIPVELFQVLKDDAVKVLHSICQQIWKTQQWPQDWKMSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIASWITKKARAFQKNIYFCFIDYAKAFDCVDHNKLENSERDGNTRPPDLPLEKPVCRSGATVRTGHGTTDWFQIGKGVHQVCILSPCLFNLYAEYIMRNAGPDEPQAGIKIAGRNINNLRYSDDTTLMAESEELKSLLMKVKEESEKVGLKLNIQKTKIMASSPITSWQIDGETVADFIFGGSKITADGDRSHENKRCLLLGRKVMTNLDSILKSRDITLSTKVHLLKAMVFPVVMYGCESWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F4J4</accession>
    <name>A0A4W2F4J4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 178B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TMEM178B</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027394616.1">
        <property type="nucleotide sequence ID" value="XM_027538815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F4J4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032294.1">
        <property type="protein sequence ID" value="ENSBIXP00000038788.1"/>
        <property type="gene ID" value="ENSBIXG00000008061.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055065.1">
        <property type="protein sequence ID" value="ENSBIXP00005000103.1"/>
        <property type="gene ID" value="ENSBIXG00005030684.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891124"/>
    <dbReference type="CTD" id="100507421"/>
    <dbReference type="GeneTree" id="ENSGT00390000015299"/>
    <dbReference type="OMA" id="RQHNSTN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039625">
        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611273" evidence="8">
        <location>
            <begin position="24"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="294" mass="33363" checksum="813DE2D48D888ED0" modified="2019-09-18" version="1">MAAGRLLLYTGLSLALCALGMLAVAICSDHWYETDARKHRDRCKAFNTRRIDPGFIYNNNNNLPLRASRSRLDRWEGKLLRARNRRQLFAMSPADECSRQYNSTNMGLWRKCHRQGFDPEIAALIRKGEIERCTYIKYHYSSATIPRNLTFNITKTIRQDEWHALHLRRMTAGFMGMAVAIILFGWIIGVLGCCWDRGLMQYVAGLLFLMGGTFCIISLCTCVAGINFELSRYPRYLYGLPDDISHGYGWSMFCAWGGLGLTLISGFFCTLAPSVQPVPRTNCPKSRPENGTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F7U9</accession>
    <name>A0A4W2F7U9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Histone arginine demethylase JMJD6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">JmjC domain-containing protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Jumonji domain-containing protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Lysyl-hydroxylase JMJD6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Peptide-lysine 5-dioxygenase JMJD6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Phosphatidylserine receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">JMJD6</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-lysyl-[protein] + 2-oxoglutarate + O2 = (5S)-5-hydroxy-L-lysyl-[protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:58360"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15144"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:141843"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 2-oxoglutarate + 2 O2 = L-arginyl-[protein] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:58348"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2-oxoglutarate + O2 = N(omega)-methyl-L-arginyl-[protein] + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:58472"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 5'-end methyltriphosphate-guanosine-ribonucleotide-snRNA + 2-oxoglutarate + O2 = a 5'-end triphospho-guanosine-ribonucleotide-snRNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58784"/>
            <dbReference type="Rhea" id="RHEA-COMP:15220"/>
            <dbReference type="Rhea" id="RHEA-COMP:15221"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138278"/>
            <dbReference type="ChEBI" id="CHEBI:142789"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Homooligomerizes; requires lysyl-hydroxylase activity. Interacts with LUC7L2, LUC7L3 and U2AF2/U2AF65. Interacts with CDK9 and CCNT1; the interaction is direct with CDK9 and associates the P-TEFb complex when active. Interacts (via JmjC and N-terminal domains) with BRD4 (via NET domain); the interaction is stronger in presence of ssRNA and recruits JMJD6 on distal enhancers. Interacts with ARGLU1; interaction may be involved in ARGLU1-mediated modulation of alternative splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the JMJD6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027374359.1">
        <property type="nucleotide sequence ID" value="XM_027518558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F7U9"/>
    <dbReference type="SMR" id="A0A4W2F7U9"/>
    <dbReference type="STRING" id="30522.A0A4W2F7U9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001795.1">
        <property type="protein sequence ID" value="ENSBIXP00000007464.1"/>
        <property type="gene ID" value="ENSBIXG00000013409.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014451.1">
        <property type="protein sequence ID" value="ENSBIXP00005001323.1"/>
        <property type="gene ID" value="ENSBIXG00005029269.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877905"/>
    <dbReference type="CTD" id="23210"/>
    <dbReference type="GeneTree" id="ENSGT00940000156867"/>
    <dbReference type="OMA" id="NAWVAMR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033749">
        <property type="term" value="F:histone H4R3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.270:FF:000001">
        <property type="entry name" value="Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000010">
        <property type="entry name" value="bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF32">
        <property type="entry name" value="BIFUNCTIONAL ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="25">
        <location>
            <begin position="141"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="336"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="414" mass="47691" checksum="AD0131D336EDB4E5" modified="2019-09-18" version="1">MNHKSKKRIREAKRSARPELKDSLDWTRHNYFESFPLNPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTREEGGNQQDEAITWFNIIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSECESGSEGEGTMHRRKKRRTCGMVGNGDTTSQDDCVSKERSSSRIRDTCGGRAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FEK5</accession>
    <name>A0A4W2FEK5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Lysosomal membrane ascorbate-dependent ferrireductase CYB561A3</fullName>
            <ecNumber evidence="18">7.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Cytochrome b ascorbate-dependent protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Lysosomal cytochrome b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CYB561A3</name>
        <name evidence="26" type="synonym">LOC113886453</name>
    </gene>
    <organism evidence="27 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Transmembrane reductase that uses ascorbate as an electron donor in the cytoplasm and transfers electrons across membranes to reduce iron cations Fe(3+) into Fe(2+) in the lumen of the late endosome and lysosome. Reduced iron can then be extruded from the late endosome and lysosome to the cytoplasm by divalent metal-specific transporters. It is therefore most probably involved in endosomal and lysosomal cellular iron homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30403"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:59513"/>
            <dbReference type="EC" id="7.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:30404"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.2.1.3" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2FEK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012462.1">
        <property type="protein sequence ID" value="ENSBIXP00000027736.1"/>
        <property type="gene ID" value="ENSBIXG00000008659.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010415.1">
        <property type="protein sequence ID" value="ENSBIXP00005003383.1"/>
        <property type="gene ID" value="ENSBIXG00005000536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183197"/>
    <dbReference type="OMA" id="LSTIYWM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140571">
        <property type="term" value="F:transmembrane ascorbate ferrireductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF38">
        <property type="entry name" value="LYSOSOMAL MEMBRANE ASCORBATE-DEPENDENT FERRIREDUCTASE CYB561A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="15 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="25">
        <location>
            <begin position="12"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="242" mass="27311" checksum="D2BBE02FF7E12BCB" modified="2019-09-18" version="1">MAVGWFYLSVLALCSLGSMCILFTIYWMRYWHGGFAWDGSMLMFNWHPVLMVTGMVVLYSAASLVYRLPQSWVGPRLPWKSGHAAMHLLAFLLTVLGLHAVFEFHNHAKIPHLYSLHSWLGITTVFLFACQWFLGFSVFLLPWASMWLRSLLKPIHVFFGASILSLAIASVVSGINEKLFFSLKNGTKTYSNLPSEAVFANCAGMLVVVFGLLVLYILLASSWKRPEPGMQAEREALLRGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FJS1</accession>
    <name>A0A4W2FJS1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2FJS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018566.1">
        <property type="protein sequence ID" value="ENSBIXP00000009984.1"/>
        <property type="gene ID" value="ENSBIXG00000007698.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007531.1">
        <property type="protein sequence ID" value="ENSBIXP00005004841.1"/>
        <property type="gene ID" value="ENSBIXG00005010672.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="OMA" id="CALMTHM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="409" mass="47256" checksum="D31C9EEB5F8AF6A2" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQAVIRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKIRKEMGIPDHLTSLLRNLYAGQEATVRTRHGTTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNTGLEETQAGIKTAGRNINNLRYADDTTLIAESEEELNSILMKGKEESEKVGLKLNIQKTKIMASSPITSWQIDGQTVETVADFIFGVSKIIADGDYSHEIKRCLLLGRKVMTNLDSILKSRDITFSTKVHLVKAMVFPVVMYGCESWTIKKAEYQRIDAFQLWCWRRLLRVPWTTRRSNQSILKEISPGCSLEGLMLKLKLQYFGHLMLQRADSFEKTLMLGKIGGRRRRGRQRMRWLDGITDSMDMSLGRLRQLVMDREAWCAAVYGVAKSWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FQT7</accession>
    <name>A0A4W2FQT7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PSMA4</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376979.1">
        <property type="nucleotide sequence ID" value="XM_027521178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FQT7"/>
    <dbReference type="SMR" id="A0A4W2FQT7"/>
    <dbReference type="STRING" id="30522.A0A4W2FQT7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002415.1">
        <property type="protein sequence ID" value="ENSBIXP00000007137.1"/>
        <property type="gene ID" value="ENSBIXG00000013136.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005003391.1">
        <property type="protein sequence ID" value="ENSBIXP00005006950.1"/>
        <property type="gene ID" value="ENSBIXG00005012348.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879564"/>
    <dbReference type="CTD" id="5685"/>
    <dbReference type="GeneTree" id="ENSGT00550000074827"/>
    <dbReference type="OMA" id="YVLNDNM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000022">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 7 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="10">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="261" mass="29484" checksum="7867422B1B31F3B9" modified="2019-09-18" version="1">MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FWD5</accession>
    <name>A0A4W2FWD5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC113897490</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2FWD5"/>
    <dbReference type="STRING" id="30522.A0A4W2FWD5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034023.1">
        <property type="protein sequence ID" value="ENSBIXP00000020103.1"/>
        <property type="gene ID" value="ENSBIXG00000023474.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044419.1">
        <property type="protein sequence ID" value="ENSBIXP00005008902.1"/>
        <property type="gene ID" value="ENSBIXG00005006215.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064170"/>
    <dbReference type="OMA" id="QHTHHFL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058745">
        <property type="entry name" value="PWI_Topors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TOPORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077:SF3">
        <property type="entry name" value="TOPOISOMERASE I BINDING, ARGININE_SERINE-RICH LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26084">
        <property type="entry name" value="PWI_Topors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Topors PWI-like" evidence="5">
        <location>
            <begin position="169"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="600"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="664"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="612"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="712"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="730" mass="83935" checksum="C2319445F01D1B60" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G159</accession>
    <name>A0A4W2G159_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Keratin 78</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KRT78</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398765.1">
        <property type="nucleotide sequence ID" value="XM_027542964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G159"/>
    <dbReference type="SMR" id="A0A4W2G159"/>
    <dbReference type="STRING" id="30522.A0A4W2G159"/>
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        <property type="protein sequence ID" value="ENSBIXP00000038697.1"/>
        <property type="gene ID" value="ENSBIXG00000007530.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045695.1">
        <property type="protein sequence ID" value="ENSBIXP00005011486.1"/>
        <property type="gene ID" value="ENSBIXG00005002503.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893185"/>
    <dbReference type="CTD" id="196374"/>
    <dbReference type="GeneTree" id="ENSGT00940000162461"/>
    <dbReference type="OMA" id="ETQQIRT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF18">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="105"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="160"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="316"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005011486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="528" mass="57464" checksum="F0AB6E80013185AA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GC93</accession>
    <name>A0A4W2GC93_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AP4B1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with TEPSIN; this interaction requires the presence of a functional AP-4 complex. Interacts with GRIA2; probably indirect it mediates the somatodendritic localization of GRIA2 in neurons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027391885.1">
        <property type="nucleotide sequence ID" value="XM_027536084.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027391893.1">
        <property type="nucleotide sequence ID" value="XM_027536092.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027391897.1">
        <property type="nucleotide sequence ID" value="XM_027536096.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027391898.1">
        <property type="nucleotide sequence ID" value="XM_027536097.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027391899.1">
        <property type="nucleotide sequence ID" value="XM_027536098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GC93"/>
    <dbReference type="SMR" id="A0A4W2GC93"/>
    <dbReference type="STRING" id="30522.A0A4W2GC93"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034841.1">
        <property type="protein sequence ID" value="ENSBIXP00000020637.1"/>
        <property type="gene ID" value="ENSBIXG00000023927.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047181.1">
        <property type="protein sequence ID" value="ENSBIXP00005014476.1"/>
        <property type="gene ID" value="ENSBIXG00005002992.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889424"/>
    <dbReference type="CTD" id="10717"/>
    <dbReference type="GeneTree" id="ENSGT00940000157025"/>
    <dbReference type="OMA" id="FIQRPTR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030124">
        <property type="term" value="C:AP-4 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098541">
        <property type="term" value="C:cytoplasmic side of trans-Golgi network transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061938">
        <property type="term" value="P:protein localization to somatodendritic compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000151">
        <property type="entry name" value="AP complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000013">
        <property type="entry name" value="AP complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015151">
        <property type="entry name" value="B-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09066">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01020">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Beta-adaptin appendage C-terminal subdomain" evidence="10">
        <location>
            <begin position="620"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="739" mass="82892" checksum="0961598427C60B71" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GG73</accession>
    <name>A0A4W2GG73_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat shock protein beta-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HSPB2</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May regulate the kinase DMPK.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DMPK; may enhance its kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419579.1">
        <property type="nucleotide sequence ID" value="XM_027563778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GG73"/>
    <dbReference type="SMR" id="A0A4W2GG73"/>
    <dbReference type="STRING" id="30522.A0A4W2GG73"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007486.1">
        <property type="protein sequence ID" value="ENSBIXP00000004598.1"/>
        <property type="gene ID" value="ENSBIXG00000000723.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028630.1">
        <property type="protein sequence ID" value="ENSBIXP00005017010.1"/>
        <property type="gene ID" value="ENSBIXG00005020506.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905862"/>
    <dbReference type="CTD" id="3316"/>
    <dbReference type="GeneTree" id="ENSGT00940000161288"/>
    <dbReference type="OMA" id="MSTEYEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007525">
        <property type="term" value="P:somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06476">
        <property type="entry name" value="ACD_HspB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000025">
        <property type="entry name" value="heat shock protein beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003090">
        <property type="entry name" value="Alpha-crystallin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF27">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00525">
        <property type="entry name" value="Crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="13">
        <location>
            <begin position="55"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20190" checksum="7026DCE0C1619CCD" modified="2019-09-18" version="1">MSGRSVPHAHPATAEYEFANPSRLGEQRFGEGLLPEEILTPTLYHGYYVRPRAAPGGEGSRAGASELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYVLPADVDPWRVRAALSHDGILNLEAPRGGRHLDTEVNEVYISLLPAPPDPEEEEEAVGVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GKV8</accession>
    <name>A0A4W2GKV8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">General transcription and DNA repair factor IIH helicase subunit XPD</fullName>
            <ecNumber evidence="19">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">CXPD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">DNA 5'-3' helicase XPD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">DNA excision repair protein ERCC-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">DNA repair protein complementing XP-D cells</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Xeroderma pigmentosum group D-complementing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ERCC2</name>
    </gene>
    <organism evidence="28 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="19"/>
    <dbReference type="RefSeq" id="XP_027371279.1">
        <property type="nucleotide sequence ID" value="XM_027515478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GKV8"/>
    <dbReference type="SMR" id="A0A4W2GKV8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022088.1">
        <property type="protein sequence ID" value="ENSBIXP00000012272.1"/>
        <property type="gene ID" value="ENSBIXG00000002895.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031266.1">
        <property type="protein sequence ID" value="ENSBIXP00005018754.1"/>
        <property type="gene ID" value="ENSBIXG00005022070.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876348"/>
    <dbReference type="CTD" id="2068"/>
    <dbReference type="GeneTree" id="ENSGT00950000182970"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035315">
        <property type="term" value="P:hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17969">
        <property type="entry name" value="DEAHc_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000381">
        <property type="entry name" value="TFIIH basal transcription factor complex helicase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
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        <property type="entry name" value="Helicase-like_DEXD_c2"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
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        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
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        <property type="entry name" value="RAD3/XPD"/>
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        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-0347">Helicase</keyword>
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    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
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        <location>
            <begin position="7"/>
            <end position="283"/>
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        <location>
            <begin position="249"/>
            <end position="276"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079246"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081072"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00082576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="760" mass="86999" checksum="645BE334EA415BC4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GW69</accession>
    <name>A0A4W2GW69_BOBOX</name>
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        <recommendedName>
            <fullName evidence="13">Pannexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PANX3</name>
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    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
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            <taxon>Bovinae</taxon>
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    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="13">Structural component of the gap junctions and the hemichannels.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <location>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU010713"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="392" mass="44585" checksum="8CB1EA32C084882A" modified="2019-09-18" version="1">MSLAHTAAEYMLSDALLPDRKGPRLKGLRLELPLDRMVKFVAVGFPLLLVSLAFAQEFSSGSPISCFCPSNFSIRQAAYVDSSCWDSLVHHEQDESGQSKTKSLWPHKALPYSLLALAVAMYLPVLLWQYAAVPALSSDLLFIISELDKSYNRAIRLVQHMLKIRQESSDTDVFWDELEKARKERYFEFPLLERYLACKQRSHSLVATYLLRNALLLLFTSATYLYLGHFHLDVFFQEEFSCSIKTGLLHDETHIPDLITCRLTSLSVFQIVSLASVAIYTLLVPVILYNLTRLCRWDKRLLSIYEMLPAFDLLSRKMLGCPINDLNVILLFLRANISELVSFSWLSVLCALKDTATQKHNIDTVVDFMTLLAGLEPSKPKHLTHGMCDENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GX86</accession>
    <name>A0A4W2GX86_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">FRA10A associated CGG repeat 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">FRA10AC1</name>
        <name evidence="2" type="synonym">LOC113884606</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GX86"/>
    <dbReference type="STRING" id="30522.A0A4W2GX86"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002304.1">
        <property type="protein sequence ID" value="ENSBIXP00000031036.1"/>
        <property type="gene ID" value="ENSBIXG00000013180.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051842.1">
        <property type="protein sequence ID" value="ENSBIXP00005023358.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 26"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019129">
        <property type="entry name" value="Folate-sensitive_fs_Fra10Ac1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF25">
        <property type="entry name" value="PROTEIN FRA10AC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09725">
        <property type="entry name" value="Fra10Ac1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="315" mass="37503" checksum="8211FD9E75C01438" modified="2019-09-18" version="1">MHGHGGYDSDFSDDEHCGESSKRKKRTVEDDLLLKKPFQKEKHGKVVHKQVAAELLDREEAKNRRFHLIAMDAYQRHTKFVNDYILYYGGKKEDFRRLGENDKTDMDVIRENHRFLWNEEDEMDMNWEKRLAKKYYDKLFKEYCIADLSRYKENKFGFRWRIEKEVISGKGQFCCGNKSCDKKEGLKSWEVNFGYTEHGEKRNALVKLRLCQECSFKLNFHHRRKEIKSKKRKDKAEKDSEESTHKKSRLSSVEETSKTKDKAHSSSVKSENSVNRNSDEEESASESELWKGPLPETDEKSQEEEFDEYFQDLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H1Q4</accession>
    <name>A0A4W2H1Q4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 116</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMEM116</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027422410.1">
        <property type="nucleotide sequence ID" value="XM_027566609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H1Q4"/>
    <dbReference type="STRING" id="30522.A0A4W2H1Q4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016414.1">
        <property type="protein sequence ID" value="ENSBIXP00000031984.1"/>
        <property type="gene ID" value="ENSBIXG00000014427.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052737.1">
        <property type="protein sequence ID" value="ENSBIXP00005024931.1"/>
        <property type="gene ID" value="ENSBIXG00005027493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907447"/>
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    <dbReference type="GeneTree" id="ENSGT00390000003209"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="78"/>
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            <begin position="203"/>
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        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
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        <location>
            <begin position="36"/>
            <end position="311"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="364" mass="41657" checksum="FA3AD8E740614998" modified="2019-09-18" version="1">MAFLLETSILEDSWDLFFSAIQWIQFVMATLSVIGSSSLVAYAIFQNIQKSPERRPLFYLSFSDLLLGIYWLTEALLYGTSTASKDIVCYNLQVVGQVLYISSFFYTINYIWYLYKELRMKPSQSAQRTFSLVIDYTCRIGQIAIILSSLIPLLLMVPVFCLGNVSECFRNFSQSHRCILIYSPPPAMAELLPSANTSVCSTLYFYGLIIFLASFVLSFFTIVVLLIQAQILYKKFLKSTGFLGSEQWAVIHIMEQRVRFYPVAFFCCWSPVVILMIIKLTKLQDTRLHMALYVLQALTTSSQGLLNCGVYGWTQYKFYQLKQEARRDADTQTPLLCSQKRFYCRGLDPLESTLAFATSTSTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H2Z7</accession>
    <name>A0A4W2H2Z7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Ribonuclease P/MRP protein subunit POP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">POP5</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027422367.1">
        <property type="nucleotide sequence ID" value="XM_027566566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H2Z7"/>
    <dbReference type="SMR" id="A0A4W2H2Z7"/>
    <dbReference type="STRING" id="30522.A0A4W2H2Z7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043631.1">
        <property type="protein sequence ID" value="ENSBIXP00000008446.1"/>
        <property type="gene ID" value="ENSBIXG00000014295.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042264.1">
        <property type="protein sequence ID" value="ENSBIXP00005026194.1"/>
        <property type="gene ID" value="ENSBIXG00005028572.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907422"/>
    <dbReference type="CTD" id="51367"/>
    <dbReference type="GeneTree" id="ENSGT00390000012331"/>
    <dbReference type="OMA" id="MQNYLDK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3250:FF:000001">
        <property type="entry name" value="Ribonuclease P/MRP protein subunit POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48414">
        <property type="entry name" value="POP5 HOMOLOG, RIBONUCLEASE P_MRP SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48414:SF1">
        <property type="entry name" value="POP5 HOMOLOG, RIBONUCLEASE P_MRP SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="170" mass="19351" checksum="712668C4F7745A8C" modified="2019-09-18" version="1">MVRFKHRYLLCEVVSDDPRCRLTLEDRVLGTLVRDTIARVHGTFGAAACSIGFAVRYLNAYTGIVLLRCRKEFYRLVWSALPFITSLENKGHRYPCFLNTLHVGGTIRTCQKFLIQYNRRQLLILLQNCTDEGEREAIQKSVTKSCLLEEESAGEELSDSGGEETAEPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H2Z9</accession>
    <name>A0A4W2H2Z9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA (guanine(27)-N(2))-dimethyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">tRNA methyltransferase 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TRMT1L</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>guanosine(27) in tRNA(Tyr) + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(27) in tRNA(Tyr) + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:83895"/>
            <dbReference type="Rhea" id="RHEA-COMP:20240"/>
            <dbReference type="Rhea" id="RHEA-COMP:20241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:83896"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027421379.1">
        <property type="nucleotide sequence ID" value="XM_027565578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H2Z9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015898.1">
        <property type="protein sequence ID" value="ENSBIXP00000031763.1"/>
        <property type="gene ID" value="ENSBIXG00000014168.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039196.1">
        <property type="protein sequence ID" value="ENSBIXP00005024087.1"/>
        <property type="gene ID" value="ENSBIXG00005004576.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906866"/>
    <dbReference type="CTD" id="81627"/>
    <dbReference type="GeneTree" id="ENSGT00530000063646"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000098">
        <property type="entry name" value="Trmt1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF1">
        <property type="entry name" value="TRMT1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 19" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 19" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="737" mass="81844" checksum="F890DCDCC7C4AD83" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HE00</accession>
    <name>A0A4W2HE00_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Leucine rich repeat LGI family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LGI4</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027370060.1">
        <property type="nucleotide sequence ID" value="XM_027514259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HE00"/>
    <dbReference type="SMR" id="A0A4W2HE00"/>
    <dbReference type="STRING" id="30522.A0A4W2HE00"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020105.1">
        <property type="protein sequence ID" value="ENSBIXP00000011005.1"/>
        <property type="gene ID" value="ENSBIXG00000002523.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013886.1">
        <property type="protein sequence ID" value="ENSBIXP00005028438.1"/>
        <property type="gene ID" value="ENSBIXG00005008095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875656"/>
    <dbReference type="CTD" id="163175"/>
    <dbReference type="GeneTree" id="ENSGT00940000162018"/>
    <dbReference type="OMA" id="WLLQWMA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014009">
        <property type="term" value="P:glial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022011">
        <property type="term" value="P:myelination in peripheral nervous system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042551">
        <property type="term" value="P:neuron maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031641">
        <property type="term" value="P:regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000017">
        <property type="entry name" value="leucine-rich repeat LGI family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009039">
        <property type="entry name" value="EAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005492">
        <property type="entry name" value="EPTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051295">
        <property type="entry name" value="LGI_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24367:SF268">
        <property type="entry name" value="LEUCINE-RICH REPEAT LGI FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24367">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03736">
        <property type="entry name" value="EPTP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01463">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50912">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611880" evidence="8">
        <location>
            <begin position="21"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="9">
        <location>
            <begin position="159"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="211"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="257"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="303"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="352"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="397"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="442"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="EAR" evidence="7">
        <location>
            <begin position="488"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="538" mass="59611" checksum="E0811847DAE1B4B5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HE14</accession>
    <name>A0A4W2HE14_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sestrin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SESN1</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a hydroperoxide + L-cysteinyl-[protein] = S-hydroxy-L-cysteinyl-[protein] + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:67124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17193"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:67125"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sestrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027407058.1">
        <property type="nucleotide sequence ID" value="XM_027551257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HE14"/>
    <dbReference type="SMR" id="A0A4W2HE14"/>
    <dbReference type="STRING" id="30522.A0A4W2HE14"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020252.1">
        <property type="protein sequence ID" value="ENSBIXP00000011084.1"/>
        <property type="gene ID" value="ENSBIXG00000016444.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013844.1">
        <property type="protein sequence ID" value="ENSBIXP00005028454.1"/>
        <property type="gene ID" value="ENSBIXG00005008131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898268"/>
    <dbReference type="CTD" id="27244"/>
    <dbReference type="GeneTree" id="ENSGT00950000183168"/>
    <dbReference type="OMA" id="CDQVTQV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098869">
        <property type="term" value="P:cellular oxidant detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1290.10:FF:000001">
        <property type="entry name" value="Sestrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF3">
        <property type="entry name" value="SESTRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="551" mass="63895" checksum="3C2BE7C684EE2494" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HEY7</accession>
    <name>A0A4W2HEY7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-pyruvoyl tetrahydrobiopterin synthase</fullName>
            <ecNumber evidence="4">4.2.3.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTPS family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2HEY7"/>
    <dbReference type="SMR" id="A0A4W2HEY7"/>
    <dbReference type="STRING" id="30522.A0A4W2HEY7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013287.1">
        <property type="protein sequence ID" value="ENSBIXP00000001757.1"/>
        <property type="gene ID" value="ENSBIXG00000008300.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012916.1">
        <property type="protein sequence ID" value="ENSBIXP00005028754.1"/>
        <property type="gene ID" value="ENSBIXG00005008500.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002752"/>
    <dbReference type="OMA" id="HFNAAHK"/>
    <dbReference type="UniPathway" id="UPA00849">
        <property type="reaction ID" value="UER00819"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00470">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.10:FF:000003">
        <property type="entry name" value="6-pyruvoyl tetrahydrobiopterin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022470">
        <property type="entry name" value="PTPS_Cys_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00039">
        <property type="entry name" value="6PTHBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00987">
        <property type="entry name" value="PTPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="165" mass="18643" checksum="446180D6E692AE36" modified="2019-09-18" version="1">MNGAGLGPGWWARVSRLVSFSASHRLHSKSLSNEENLKLFGKCNNPNGHGHNYKVVVTVHGEVDPVTGMVMNLTDLKEYIEEAIMKPLDHKNLDLGVPYFADIVSTTENVAVYIWENLQKFLPVGVLYKVKVYETDNNIVVYKGEQLLEISFHPYLKKIFIPLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HRA5</accession>
    <name>A0A4W2HRA5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-MNLL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase MNLL subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB1 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HRA5"/>
    <dbReference type="SMR" id="A0A4W2HRA5"/>
    <dbReference type="STRING" id="30522.A0A4W2HRA5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000692.1">
        <property type="protein sequence ID" value="ENSBIXP00000007977.1"/>
        <property type="gene ID" value="ENSBIXG00000013846.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021419.1">
        <property type="protein sequence ID" value="ENSBIXP00005035332.1"/>
        <property type="gene ID" value="ENSBIXG00005016459.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170401"/>
    <dbReference type="OMA" id="AREHWVN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012575">
        <property type="entry name" value="NDUB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15222">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15222:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08040">
        <property type="entry name" value="NADH_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="58" mass="6965" checksum="765D588A1D3F86A4" modified="2019-09-18" version="1">MMNLLQIMCDHWVHSHVPVGLVFRCYLDRKNDGKLTAFRNKNLLYKRKLKSSEEVTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HRQ0</accession>
    <name>A0A4W2HRQ0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Zinc finger and BTB domain containing 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZBTB39</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398086.1">
        <property type="nucleotide sequence ID" value="XM_027542285.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398087.1">
        <property type="nucleotide sequence ID" value="XM_027542286.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027398088.1">
        <property type="nucleotide sequence ID" value="XM_027542287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HRQ0"/>
    <dbReference type="STRING" id="30522.A0A4W2HRQ0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026306.1">
        <property type="protein sequence ID" value="ENSBIXP00000015068.1"/>
        <property type="gene ID" value="ENSBIXG00000003517.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001321.1">
        <property type="protein sequence ID" value="ENSBIXP00005032571.1"/>
        <property type="gene ID" value="ENSBIXG00005013102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892855"/>
    <dbReference type="CTD" id="9880"/>
    <dbReference type="GeneTree" id="ENSGT00940000160722"/>
    <dbReference type="OMA" id="SSFVDWH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18224">
        <property type="entry name" value="BTB_POZ_ZBTB39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001792">
        <property type="entry name" value="Zinc finger and BTB domain-containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001375">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000104">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047989">
        <property type="entry name" value="ZBTB39_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF43">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="16">
        <location>
            <begin position="30"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="600"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="656"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="134"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="707" mass="78635" checksum="EF3D01121D43046F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HS75</accession>
    <name>A0A4W2HS75_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">SCN8A</name>
    </gene>
    <organism evidence="29 31">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient. Contributes to neuronal excitability by regulating action potential threshold and propagation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 23">Cell membrane</location>
            <topology evidence="2 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398718.1">
        <property type="nucleotide sequence ID" value="XM_027542917.1"/>
    </dbReference>
    <dbReference type="STRING" id="30522.A0A4W2HS75"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031753.1">
        <property type="protein sequence ID" value="ENSBIXP00000018613.1"/>
        <property type="gene ID" value="ENSBIXG00000022416.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005022325.1">
        <property type="protein sequence ID" value="ENSBIXP00005035689.1"/>
        <property type="gene ID" value="ENSBIXG00005016926.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893153"/>
    <dbReference type="CTD" id="6334"/>
    <dbReference type="GeneTree" id="ENSGT00940000156263"/>
    <dbReference type="OMA" id="DPFYHNQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031402">
        <property type="term" value="F:sodium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1190:FF:000003">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008054">
        <property type="entry name" value="Na_channel_a8su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF23">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 8 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01667">
        <property type="entry name" value="NACHANNEL8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19 23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 23" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 23" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 23" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="17 23" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 23" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 23" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="754"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="784"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="864"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="951"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1199"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1238"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1269"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1313"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1438"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1523"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1553"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1583"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1640"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1743"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="131"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="27">
        <location>
            <begin position="546"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="753"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium ion transport-associated" evidence="26">
        <location>
            <begin position="991"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1197"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1522"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="28">
        <location>
            <begin position="1888"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="446"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="568"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1107"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1922"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="471"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="501"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="586"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1938"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1951"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00058180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF06512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF11933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF24609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1980" mass="225302" checksum="C30683E5E47B2886" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HTT0</accession>
    <name>A0A4W2HTT0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Protein SYS1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SYS1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein trafficking. May serve as a receptor for ARFRP1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with ARFRP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414254.1">
        <property type="nucleotide sequence ID" value="XM_027558453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HTT0"/>
    <dbReference type="STRING" id="30522.A0A4W2HTT0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024914.1">
        <property type="protein sequence ID" value="ENSBIXP00000035651.1"/>
        <property type="gene ID" value="ENSBIXG00000018898.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019946.1">
        <property type="protein sequence ID" value="ENSBIXP00005034670.1"/>
        <property type="gene ID" value="ENSBIXG00005015706.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902982"/>
    <dbReference type="CTD" id="90196"/>
    <dbReference type="GeneTree" id="ENSGT00940000154347"/>
    <dbReference type="OMA" id="EYEMVGM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016973">
        <property type="entry name" value="Integral_membrane_SYS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031402">
        <property type="entry name" value="SYS1_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR031402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17445" checksum="E212B699018DCF3E" modified="2019-09-18" version="1">MAGQFRSYVWDPLLILSQIVLMQTVYYGSLGLWLALVDGLVHSSPSLDQVFDAEILGFSTPPGRLSTMSFVLNALTCALGLLYFIRRGKQCLDFTVTVHFFHLLGCWLYSSRFPSALTWWLVQAVCIALMAVIGEYLCMRSELKEIPLNSAPKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HUR9</accession>
    <name>A0A4W2HUR9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HUR9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006060.1">
        <property type="protein sequence ID" value="ENSBIXP00000029776.1"/>
        <property type="gene ID" value="ENSBIXG00000011511.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021286.1">
        <property type="protein sequence ID" value="ENSBIXP00005035261.1"/>
        <property type="gene ID" value="ENSBIXG00005016373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000147716"/>
    <dbReference type="OMA" id="WKIRWRL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="87" mass="10466" checksum="6D3C3DEADD8378A1" modified="2025-10-08" version="2">MTSQHLIMTRPSLQVWFSSSPHVWKIRWRLGDTHLWRRGDGEREEEGRKREKRLVFAEVDKLTSTRECHIWKCVACPDSLKPLIYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYR2</accession>
    <name>A0A4W2HYR2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA damage-inducible transcript 4 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DDIT4</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Monomer. Interacts with BTRC. Identified in a complex with CUL4A, DDB1 and BTRC. Interacts with TXNIP; this inhibits the proteasomal degradation of DDIT4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386431.1">
        <property type="nucleotide sequence ID" value="XM_027530630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HYR2"/>
    <dbReference type="SMR" id="A0A4W2HYR2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007814.1">
        <property type="protein sequence ID" value="ENSBIXP00000004438.1"/>
        <property type="gene ID" value="ENSBIXG00000010787.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005045825.1">
        <property type="protein sequence ID" value="ENSBIXP00005035041.1"/>
        <property type="gene ID" value="ENSBIXG00005016099.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885348"/>
    <dbReference type="CTD" id="54541"/>
    <dbReference type="GeneTree" id="ENSGT00530000063652"/>
    <dbReference type="OMA" id="MPGLWER"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071549">
        <property type="term" value="P:cellular response to dexamethasone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032984">
        <property type="term" value="P:protein-containing complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.40:FF:000001">
        <property type="entry name" value="DNA damage-inducible transcript 4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF7">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="229" mass="24811" checksum="1AB4CB2849F9AB6B" modified="2019-09-18" version="1">MPSLWDRFSSSSSSSSLSRTPTPNQPPRSAWGSAAREEGLGRCGSLESSDCESLDSSNSGFGPEEDSAYLDGVSLPDFELLSDPEDEHLCASLMQLLQESLAQARLGSRRPARLLMPGQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFVPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I9W7</accession>
    <name>A0A4W2I9W7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">RAB2A, member RAS onco family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RAB2A</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasmic vesicle</location>
            <location evidence="14">Autophagosome membrane</location>
            <topology evidence="14">Lipid-anchor</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416842.1">
        <property type="nucleotide sequence ID" value="XM_027561041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I9W7"/>
    <dbReference type="SMR" id="A0A4W2I9W7"/>
    <dbReference type="STRING" id="30522.A0A4W2I9W7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022562.1">
        <property type="protein sequence ID" value="ENSBIXP00000012580.1"/>
        <property type="gene ID" value="ENSBIXG00000017643.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031410.1">
        <property type="protein sequence ID" value="ENSBIXP00005039729.1"/>
        <property type="gene ID" value="ENSBIXG00005022188.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904202"/>
    <dbReference type="CTD" id="5862"/>
    <dbReference type="GeneTree" id="ENSGT00940000153886"/>
    <dbReference type="OMA" id="TNATHAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01866">
        <property type="entry name" value="Rab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000275">
        <property type="entry name" value="Putative ras-related protein Rab-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="212" mass="23519" checksum="F8731E25A6B399A3" modified="2019-09-18" version="1">MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNATHAGSQGGQQAGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IAH5</accession>
    <name>A0A4W2IAH5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Interferon regulatory factor 2 binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IRF2BP2</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRF2BP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027386178.1">
        <property type="nucleotide sequence ID" value="XM_027530377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IAH5"/>
    <dbReference type="SMR" id="A0A4W2IAH5"/>
    <dbReference type="STRING" id="30522.A0A4W2IAH5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004875.1">
        <property type="protein sequence ID" value="ENSBIXP00000030164.1"/>
        <property type="gene ID" value="ENSBIXG00000012019.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034149.1">
        <property type="protein sequence ID" value="ENSBIXP00005040921.1"/>
        <property type="gene ID" value="ENSBIXG00005023732.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885243"/>
    <dbReference type="CTD" id="359948"/>
    <dbReference type="GeneTree" id="ENSGT00940000160591"/>
    <dbReference type="OMA" id="FKKEPGM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002327">
        <property type="term" value="P:immature B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16716">
        <property type="entry name" value="vRING-HC_IRF2BP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1580:FF:000001">
        <property type="entry name" value="interferon regulatory factor 2-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1580">
        <property type="entry name" value="Interferon regulatory factor 2-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044882">
        <property type="entry name" value="I2BP1/2_C3HC4-RING_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058682">
        <property type="entry name" value="IRF-2BP1/2-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022750">
        <property type="entry name" value="IRF-2BP1_2-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057414">
        <property type="entry name" value="Zf-C3HC4_IRF-2BP1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF18">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 2-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11261">
        <property type="entry name" value="IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25457">
        <property type="entry name" value="IRF-2BP1_2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25454">
        <property type="entry name" value="zf-C3HC4_IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like zinc finger" evidence="11">
        <location>
            <begin position="12"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="IRF-2BP1/2-like middle" evidence="13">
        <location>
            <begin position="296"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like C3HC4 zinc finger" evidence="12">
        <location>
            <begin position="505"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="125"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="231"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="345"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="438"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="588" mass="60755" checksum="539D36984ED8E2E2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ICS5</accession>
    <name>A0A4W2ICS5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Outer mitochondrial membrane lipid metabolism regulator OPA3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">OPA3</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play some role in mitochondrial processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OPA3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ICS5"/>
    <dbReference type="STRING" id="30522.A0A4W2ICS5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022235.1">
        <property type="protein sequence ID" value="ENSBIXP00000012392.1"/>
        <property type="gene ID" value="ENSBIXG00000017445.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032139.1">
        <property type="protein sequence ID" value="ENSBIXP00005040048.1"/>
        <property type="gene ID" value="ENSBIXG00005022571.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009795"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="286" mass="31329" checksum="525D26BB74C84E90" modified="2019-09-18" version="1">MTSAEAGVGTHIGYSSRLTVPPSDSLTSAVYLHIHRSGRRSVSVYAVTSWTAPAVPPGRTHVRTHARRREGGGGGSDLRRPSQSARLRVPLATAKMVVGAFPMAKLLYLGIRQVSKPLANRIKEAARRSEFFKTYICLPPAQLYHWVEMRAKMRLMGFNAETIKPLNEEAAAELGAELLGEATIFLVACGCLVLEYSRQKTQQRRKEGEQLAAWDAMRDEVDHLALVLEALQAQVQAAPPPGALEELQAQMREVRAQLCVQDLPPAPQEEPTPRDEGPPDPGLGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IDD7</accession>
    <name>A0A4W2IDD7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Histone deacetylase complex subunit SAP30L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Sin3 corepressor complex subunit SAP30L</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sin3-associated protein p30-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SAP30L</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a transcription repressor, probably via its interaction with histone deacetylase complexes. Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus. Binds DNA, apparently without sequence-specificity, and bends bound double-stranded DNA. Binds phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate) via the same basic sequence motif that mediates DNA binding and nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with components of the histone deacetylase complex SIN3A, HDAC1 and HDAC2. Binds histones and nucleosomes. Interacts with FEZ1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP30 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401679.1">
        <property type="nucleotide sequence ID" value="XM_027545878.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027401680.1">
        <property type="nucleotide sequence ID" value="XM_027545879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IDD7"/>
    <dbReference type="SMR" id="A0A4W2IDD7"/>
    <dbReference type="STRING" id="30522.A0A4W2IDD7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052030.1">
        <property type="protein sequence ID" value="ENSBIXP00000025174.1"/>
        <property type="gene ID" value="ENSBIXG00000027283.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049877.1">
        <property type="protein sequence ID" value="ENSBIXP00005040230.1"/>
        <property type="gene ID" value="ENSBIXG00005003903.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895121"/>
    <dbReference type="CTD" id="79685"/>
    <dbReference type="GeneTree" id="ENSGT00390000006633"/>
    <dbReference type="OMA" id="SDQICCL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044378">
        <property type="term" value="F:non-sequence-specific DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1800.30:FF:000001">
        <property type="entry name" value="Histone deacetylase complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024145">
        <property type="entry name" value="His_deAcase_SAP30/SAP30L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025717">
        <property type="entry name" value="SAP30_zn-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP30L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286">
        <property type="entry name" value="SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13866">
        <property type="entry name" value="zf-SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 zinc-finger" evidence="18">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="19">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20835" checksum="D2A9E7BD00F9A7C9" modified="2019-09-18" version="1">MNGFSTEEDSREGPPAAPAAAPGYGQSCCLIEDGERCVRPAGNASFSKRVQKSISQKKLKLDIDKSVRHLYICDFHKNFIQSVRNKRKRKTSDDGGDSPEHDTDIPEVDLFQLQVNTLRRYKRHYKLQTRPGFNKAQLAETVSRHFRNIPVNEKETLAYFIYMVKSNKSRLDQKSESGKQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IFX3</accession>
    <name>A0A4W2IFX3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2IFX3"/>
    <dbReference type="STRING" id="30522.A0A4W2IFX3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037411.1">
        <property type="protein sequence ID" value="ENSBIXP00000022306.1"/>
        <property type="gene ID" value="ENSBIXG00000025211.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038985.1">
        <property type="protein sequence ID" value="ENSBIXP00005043023.1"/>
        <property type="gene ID" value="ENSBIXG00005026595.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000197193"/>
    <dbReference type="OMA" id="PLTSHIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="68" mass="8014" checksum="FD3725998A47520C" modified="2019-09-18" version="1">MDFSPLGSSVHRIFQTRVLEWHAIKMNHYLEWFNMYKVHVPLTSHICGRLTKRDVKLANFVEKATVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IPY6</accession>
    <name>A0A4W2IPY6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vitamin-K-epoxide reductase (warfarin-sensitive)</fullName>
            <ecNumber evidence="3">1.17.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">VKORC1L1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2IPY6"/>
    <dbReference type="STRING" id="30522.A0A4W2IPY6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014165.1">
        <property type="protein sequence ID" value="ENSBIXP00000027197.1"/>
        <property type="gene ID" value="ENSBIXG00000028635.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041572.1">
        <property type="protein sequence ID" value="ENSBIXP00005044338.1"/>
        <property type="gene ID" value="ENSBIXG00005028184.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157044"/>
    <dbReference type="OMA" id="ICIATYV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047057">
        <property type="term" value="F:vitamin-K-epoxide reductase (warfarin-sensitive) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042373">
        <property type="term" value="P:vitamin K metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12917">
        <property type="entry name" value="VKOR_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.130:FF:000001">
        <property type="entry name" value="Vitamin K epoxide reductase complex subunit 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="entry name" value="VKOR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042406">
        <property type="entry name" value="VKORC1/VKORC1L1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14519:SF5">
        <property type="entry name" value="VITAMIN K EPOXIDE REDUCTASE COMPLEX SUBUNIT 1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14519">
        <property type="entry name" value="VITAMIN K EPOXIDE REDUCTASE COMPLEX, SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Vitamin K epoxide reductase" evidence="14">
        <location>
            <begin position="137"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="64"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="301" mass="31826" checksum="AD22739109A1B1E7" modified="2019-09-18" version="1">MATPILWPRPLRAQRRLWLRPSAGLSGGCALRPLPPRPRAPSQPGRALSFRGRWAARLWGRGPEEAVEPMGRGGGGGGGGGGGGGGGSGSGWVGPRRAAAAEVEAEGGGGGGGGEGGGGGGGGGKMAAPVLLRVSVPRWERVARYAVCAAGILLSIYAYHVEREKERDPEHRALCDLGPWVKCSAALASRWGRGFGLLGSIFGKDGVLNQPNSIFGLIFYILQLLLGLTASAVAALILMTSSIMSVVGSLYLAYILYFVLKEFCIICVITYVLNFILLIINYKRLVYLNEAWKRQLQPKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IQ47</accession>
    <name>A0A4W2IQ47_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA-binding protein Musashi homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MSI1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Musashi family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027422375.1">
        <property type="nucleotide sequence ID" value="XM_027566574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IQ47"/>
    <dbReference type="SMR" id="A0A4W2IQ47"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016203.1">
        <property type="protein sequence ID" value="ENSBIXP00000008503.1"/>
        <property type="gene ID" value="ENSBIXG00000001696.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041716.1">
        <property type="protein sequence ID" value="ENSBIXP00005044417.1"/>
        <property type="gene ID" value="ENSBIXG00005028333.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907430"/>
    <dbReference type="CTD" id="4440"/>
    <dbReference type="GeneTree" id="ENSGT00940000156515"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12576">
        <property type="entry name" value="RRM1_MSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12323">
        <property type="entry name" value="RRM2_MSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000596">
        <property type="entry name" value="RNA-binding protein Musashi homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000020">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034126">
        <property type="entry name" value="MSI_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="109"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="362" mass="39125" checksum="26487B0A32CF1F40" modified="2019-09-18" version="1">METDAPQPGLASPDSPHDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSVNTTVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVMPYGMDAFMLGIGMLGYPGFQATTYASRSYTGLAPGYTYQFPEFRVERTPLPSAPVLPELTAIPLTAYGPMAAAAAAAAVVRGTGSHPWTMAPPPGSTPSRTGGFLGTTSPGPMAELYGAANQDSGVSSYISAASPAPSTGFGHSLGGPLIATAFTNGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMZ6</accession>
    <name>A0A4W2BMZ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphomannomutase</fullName>
            <ecNumber evidence="14">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PMM2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 14">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMZ6"/>
    <dbReference type="STRING" id="30522.A0A4W2BMZ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053942.1">
        <property type="protein sequence ID" value="ENSBIXP00000000048.1"/>
        <property type="gene ID" value="ENSBIXG00000030331.1"/>
    </dbReference>
    <dbReference type="OMA" id="ISHRVYT"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061728">
        <property type="term" value="P:GDP-mannose biosynthetic process from mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000216">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF2">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <begin position="15"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSBIXP00000001954.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="90" mass="10059" checksum="6F5193624EE07052" modified="2019-09-18" version="1">MSSTLARNEEKKGSRPSQPTTPATSLGCDLKVSRSEEFLTQISTELTDEALFVAAYHMNPVPIKEKQTQDRGTQISKHVFFTKTRGTDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BS26</accession>
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        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 13</fullName>
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        <name evidence="12" type="primary">MED13</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2 8">Belongs to the Mediator complex subunit 13 family.</text>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MID_MedPIWI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MID_MedPIWI"/>
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    </dbReference>
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    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="1724"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="10">
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        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2174" mass="239433" checksum="D95043882C037D9F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTR5</accession>
    <name>A0A4W2BTR5_BOBOX</name>
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        <submittedName>
            <fullName evidence="4">Golgin A4</fullName>
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    </protein>
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        <name evidence="4" type="primary">GOLGA4</name>
    </gene>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSBIXG00000009168.1"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Golgi vesicle transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.60">
        <property type="entry name" value="GRIP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327">
        <property type="entry name" value="GOLGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00755">
        <property type="entry name" value="Grip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101283">
        <property type="entry name" value="GRIP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="3">
        <location>
            <begin position="2189"/>
            <end position="2236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1759"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1939"/>
            <end position="2115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2140"/>
            <end position="2167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2251" mass="261507" checksum="ECB4E47483B9B4CD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUA0</accession>
    <name>A0A4W2BUA0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="5 12">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Interacts with TMEM35A/NACHO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Endoplasmic reticulum membrane</location>
            <topology evidence="2 12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUA0"/>
    <dbReference type="STRING" id="30522.A0A4W2BUA0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011728.1">
        <property type="protein sequence ID" value="ENSBIXP00000002539.1"/>
        <property type="gene ID" value="ENSBIXG00000009002.1"/>
    </dbReference>
    <dbReference type="OMA" id="RYEYARE"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="702" mass="77918" checksum="B5F7C111E035842E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BY23</accession>
    <name>A0A4W2BY23_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">HtrA serine peptidase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HTRA4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BY23"/>
    <dbReference type="STRING" id="30522.A0A4W2BY23"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008665.1">
        <property type="protein sequence ID" value="ENSBIXP00000004040.1"/>
        <property type="gene ID" value="ENSBIXG00000010404.1"/>
    </dbReference>
    <dbReference type="OMA" id="WIEVVLQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06785">
        <property type="entry name" value="cpPDZ_HtrA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.120:FF:000002">
        <property type="entry name" value="HtrA serine peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22939">
        <property type="entry name" value="SERINE PROTEASE FAMILY S1C HTRA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22939:SF105">
        <property type="entry name" value="SERINE PROTEASE HTRA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="482"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="414"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="482" mass="50960" checksum="F0E5B07E9FA727EE" modified="2019-09-18" version="1">MARPLQRPAGLGPFVLLWLLLPAPSGRGVQAGRAWPVPRCPAACEPTRCPPLPRCSVGTAPVLDRCGCCRVCAAAEGEACGGPLGRPCAPGLQCLAPRAPRLLGGRPLGTCGCPAAGATVCGSDGRTYRSLCALRAENRAARLRGALPAVPVQKGDCGDPGTGSAGWLRNKFNFIASVVEKVAPSVVHLQLFRRSPLGSEDVPVSSASGFIVSEDGLIVTNAHVLTNQQRIQVELQSGVQYEATVKDVDHKLDLALIKIEPNTDLPVLLLGKSSDLRAGEFVVALGSPFSLQNTVTAGIVSTTQRGGKELGLKDSDMDYIQTDAIINHGNSGGPLVNLDGDVIGINTLKVTAGISFAIPSDHIRQFLAEFHERQLKGKALSQKKYLGLRMLPLTMNLLQEMKRQDPEFPDVASGVFVHEVIQGTAAESSGLKDHDVIVSINGLPVTTTTDVIEAVKANDSLSLLVRRKSQTLILTVTPEIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZE7</accession>
    <name>A0A4W2BZE7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZE7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007130.1">
        <property type="protein sequence ID" value="ENSBIXP00000004765.1"/>
        <property type="gene ID" value="ENSBIXG00000000772.1"/>
    </dbReference>
    <dbReference type="OMA" id="KSELKWT"/>
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        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
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        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="505" mass="58758" checksum="8C6BAC91381B5CCE" modified="2019-09-18" version="1">MARVNVDILGKSELKWTGMGEFNSDDHYIYYCGQESLRRNGVAIMVNKRVQNAVLGCNLKNDRMISVHFQGKPFNITVIQVYAPTSNAEEAEAERFYEDLQDLLELTLKKDVLFIIGDWNAKVGSQETPEVTGKFGLGIRNEAGQRLIEFCQANALVIANTLFQQHKRRLYTWTSPDGQHRNQIDYILCSQRWRSSIQSAKTRPGADCGSDHELLIAKIRLKLRKIGKTTRPFRYDLNQIPYDYTVEVRNRFKGLDLKDRVPDELWTEVCDIVQETGIKTIPIEKKCKKKKAKWLSAEALQIAVKRSEAKSKGEKERYTHLNAEFQRVARRVKKAFFSNQCKEIEENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNDMDLTEAENIKKRWQEYTEELYRKDLHEPDNHDGVITDLEPDILECEVKWALESITMKKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRCFHSNPKERQCQRMLKLQHNCSHLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C149</accession>
    <name>A0A4W2C149_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C149"/>
    <dbReference type="STRING" id="30522.A0A4W2C149"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003696.1">
        <property type="protein sequence ID" value="ENSBIXP00000006461.1"/>
        <property type="gene ID" value="ENSBIXG00000012575.1"/>
    </dbReference>
    <dbReference type="OMA" id="GANRFAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18648">
        <property type="entry name" value="CD_Cbx6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF4">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="232"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
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            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="414" mass="44605" checksum="D314DD8218BA5318" modified="2019-09-18" version="1">MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPKKRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQGGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGPGAGGAGQGAGALARPKVPSRNRVIGKSKKFSESILRTQVRHMKFGTFALYNKPPPGPLQPPPAGKTDITASPGQGLLLAPPSAPYDERSSSSSDCPSPVPHSSEPEDAPPKLLPETTSPSAPDWREPEVLDLSLPPEAAATNKRAPPEVPAAASQALPTVPEPAGAASEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNPEDFEKVAAGVAGAAAGGGSSGASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1J3</accession>
    <name>A0A4W2C1J3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Centromere protein U</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">MLF1-interacting protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CENPU</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2S4</accession>
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        <name evidence="12" type="primary">LOC113892267</name>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
    <sequence length="1375" mass="150103" checksum="82927D35A63BC2D5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="14">NEK6-subfamily protein kinase</fullName>
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        </recommendedName>
    </protein>
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    </gene>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
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                <person name="Bickhart D.M."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <name>Mg(2+)</name>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140677">
        <property type="term" value="F:molecular function activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035865">
        <property type="term" value="P:cellular response to potassium ion"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070498">
        <property type="term" value="P:interleukin-1-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0035655">
        <property type="term" value="P:interleukin-18-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044546">
        <property type="term" value="P:NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050729">
        <property type="term" value="P:positive regulation of inflammatory response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032731">
        <property type="term" value="P:positive regulation of interleukin-1 beta production"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032741">
        <property type="term" value="P:positive regulation of interleukin-18 production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900227">
        <property type="term" value="P:positive regulation of NLRP3 inflammasome complex assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032206">
        <property type="term" value="P:positive regulation of telomere maintenance"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070269">
        <property type="term" value="P:pyroptotic inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08224">
        <property type="entry name" value="STKc_Nek6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000204">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000240">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="34"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="302" mass="34549" checksum="D98AF5DB34D94D4C" modified="2019-09-18" version="1">MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDITYVYDVAKRMHACTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5E4</accession>
    <name>A0A4W2C5E4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spermadhesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5E4"/>
    <dbReference type="STRING" id="30522.A0A4W2C5E4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003050.1">
        <property type="protein sequence ID" value="ENSBIXP00000006802.1"/>
        <property type="gene ID" value="ENSBIXG00000012872.1"/>
    </dbReference>
    <dbReference type="OMA" id="LHTECLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000124">
        <property type="entry name" value="Spermadhesin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00986">
        <property type="entry name" value="SPERMADHESIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="32"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="134" mass="15327" checksum="BF11237A5FEEA374" modified="2019-09-18" version="1">PPCHLDFPCFCLFLSASSDSTDGLLVKDKYLCGDLYGEEYGVIFPYLGLHTECLWIIKMDPGYRILLEVRDVHLNCNKESLEIIEGPPESSNSRKICDTSHAEYTSCTNTMTVKYTRKPNHPAPDFFLIFRRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA09</accession>
    <name>A0A4W2CA09_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC113879379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFI6/IFI27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CA09"/>
    <dbReference type="STRING" id="30522.A0A4W2CA09"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016496.1">
        <property type="protein sequence ID" value="ENSBIXP00000008698.1"/>
        <property type="gene ID" value="ENSBIXG00000014483.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009311">
        <property type="entry name" value="IFI6/IFI27-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932">
        <property type="entry name" value="INTERFERON ALPHA-INDUCIBLE PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932:SF2">
        <property type="entry name" value="INTERFERON ALPHA-INDUCIBLE PROTEIN 27, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06140">
        <property type="entry name" value="Ifi-6-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="284" mass="30455" checksum="425D1BFFF860069A" modified="2019-09-18" version="1">MPWAPSCQNCCWKVSALGKSPEGGWPRAWGSWTWAARVWVLTWCPSSLFPLGFSLLLCAVRTSSSPLVLGFSLVHLQASLTPLSSASSEVPSLHQRFWGLQERVEGAVWGLFPSCLPHCVLPHYFFLPEEEEPSQSTNQASYGSSPGKPPQGPHQGPDDFGSDPRDPPNFYHYAGMTAAAVIGGVMAVRAVPELLDTVGFTRKGISRSSLAAKMMSSTARANGGGVPSGSLVSNLQSKGATGLSTPSNILLGSAGALLGALLWDSYSSSRRTQYRSRNELPFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBF1</accession>
    <name>A0A4W2CBF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC113879868</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBF1"/>
    <dbReference type="STRING" id="30522.A0A4W2CBF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018931.1">
        <property type="protein sequence ID" value="ENSBIXP00000010223.1"/>
        <property type="gene ID" value="ENSBIXG00000015742.1"/>
    </dbReference>
    <dbReference type="OMA" id="CWGSAIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000014">
        <property type="entry name" value="Complement factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF13">
        <property type="entry name" value="CATHEPSIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021244132" description="Peptidase S1 domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="21"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="251" mass="28204" checksum="D705B095F32380E1" modified="2019-09-18" version="1">MWPLLLLVAFLLSPRAQAGQIIGGREARPHSHPYMAYIQTLTPAGPTICGGFLVREDFVMTAARCLGSQINVILGIHSVTASEWTQQRIPVLRPIPHPRYNQRNNQNDIMLLQLSNRARQNEAVRLVALPQTEEMLSPGTQCTVAGWGLTRLHRRAITLQEVQLTIQRDGECHSRFDFYTGQKQICVGDPREGKSTFLGDSGGPLVCSNVAQGVVSYGDRMGTPPAVFTRISSFLPWIRRTMRRFQEWRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBM5</accession>
    <name>A0A4W2CBM5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC113876033</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBM5"/>
    <dbReference type="STRING" id="30522.A0A4W2CBM5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009312.1">
        <property type="protein sequence ID" value="ENSBIXP00000003717.1"/>
        <property type="gene ID" value="ENSBIXG00000010116.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000050">
        <property type="entry name" value="MGC157082 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF10">
        <property type="entry name" value="MGC138914 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="369"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="600"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="629"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="658"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="11">
        <location>
            <begin position="631"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="687"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003717.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="708" mass="77182" checksum="4F46C1A6D8533129" modified="2019-09-18" version="1"> </sequence>
</entry>
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    <accession>A0A4W2CCA4</accession>
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        <submittedName>
            <fullName evidence="3">BMERB domain containing 1</fullName>
        </submittedName>
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        <name evidence="3" type="primary">BMERB1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    </dbReference>
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            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="204" mass="23599" checksum="56968C0737CC0A64" modified="2019-09-18" version="1">MELKQSLSVHLEAEKPLRRYGAVEETAWKAEGLGRNQLDIISMAETTMRPEEIELEMAKIQRLREVLVRRESELRFMMDDIQLCNDIMDLKQELQNLVAIPEKEKTKLQKQREDELIQKIHKLVQKRDFLVDDAEVERLREQEEDKEMADFLRIKLKPLDKVTKSSASSRAEKKADPPPSKPTVAKTGLALIKDCCGAAQCRIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CDW1</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Ruminantia</taxon>
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            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="865" mass="96928" checksum="74FDF4150190B4E4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CDX1</accession>
    <name>A0A4W2CDX1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">STAT5B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372677.1">
        <property type="nucleotide sequence ID" value="XM_027516876.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027372679.1">
        <property type="nucleotide sequence ID" value="XM_027516878.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027372680.1">
        <property type="nucleotide sequence ID" value="XM_027516879.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2CDX1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044970.1">
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        <property type="gene ID" value="ENSBIXG00000016479.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044013.1">
        <property type="protein sequence ID" value="ENSBIXP00005045702.1"/>
        <property type="gene ID" value="ENSBIXG00005005263.1"/>
    </dbReference>
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    <dbReference type="CTD" id="6777"/>
    <dbReference type="GeneTree" id="ENSGT01080000257420"/>
    <dbReference type="OMA" id="WIEEKMW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050798">
        <property type="term" value="P:activated T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046543">
        <property type="term" value="P:development of secondary female sexual characteristics"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046544">
        <property type="term" value="P:development of secondary male sexual characteristics"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038162">
        <property type="term" value="P:erythropoietin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007565">
        <property type="term" value="P:female pregnancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042492">
        <property type="term" value="P:gamma-delta T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060397">
        <property type="term" value="P:growth hormone receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007595">
        <property type="term" value="P:lactation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001553">
        <property type="term" value="P:luteinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097531">
        <property type="term" value="P:mast cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033028">
        <property type="term" value="P:myeloid cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001779">
        <property type="term" value="P:natural killer cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042267">
        <property type="term" value="P:natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001787">
        <property type="term" value="P:natural killer cell proliferation"/>
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    <sequence length="787" mass="89973" checksum="FEAEC3B7E1267EDE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the SCM family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
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    <dbReference type="PANTHER" id="PTHR12247:SF85">
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    <feature type="domain" description="SAM" evidence="8">
        <location>
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    </feature>
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        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="453" mass="49017" checksum="23D2B140D1A71659" modified="2019-09-18" version="1">MSTFTTTHKHPALKLLRLAAPLLPVSDLSLPSVRLAPAEMQSQRIPGRKRGRPPLHSTPVKMAVHNIYSASAGSLPAVKIPKKRGRKPGYKLKSRVLMTPLALSPPRSTPEPDLSSIPQDAATVPSLAASQALTVCLYINKQANTGPYLERRKVQQLPEHFGPERPSAVLQQAVQACIDCAHQQKLVFSLVKQGYGGEMVSVSASFDGKQHLRSLPVVNSVGYVLRFLTKLCRSLLCDDLFSHQPFPRGTSASDNAPESQEGRMESAKTVTAEECLVNPASMNRYGVDTSSPTFSHRGSLPTSSSLYCKRQNSGDGHLGGGSATTVSGPRASPLSPGGPSAPGLRPPGSSPKRNGTALEGNRCASNPSPDRQDARRPRSRNPSSWSVEDVVWFLKDADPQALGPHVELFRKHEIDGNALLLLKSDMIMKYLGLKLGPALKLCYHIDKLKQDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGG0</accession>
    <name>A0A4W2CGG0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CGG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021809.1">
        <property type="protein sequence ID" value="ENSBIXP00000012095.1"/>
        <property type="gene ID" value="ENSBIXG00000002845.1"/>
    </dbReference>
    <dbReference type="OMA" id="TYFEEHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000436">
        <property type="entry name" value="ATP binding cassette subfamily A member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF274">
        <property type="entry name" value="ABC-TYPE ORGANIC ANION TRANSPORTER ABCA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="863"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1022"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1097"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1125"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1165"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="481"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="1256"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1210"/>
            <end position="1235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1585" mass="177375" checksum="AAB100BB33D33F34" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGJ4</accession>
    <name>A0A4W2CGJ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGJ4"/>
    <dbReference type="STRING" id="30522.A0A4W2CGJ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019843.1">
        <property type="protein sequence ID" value="ENSBIXP00000010814.1"/>
        <property type="gene ID" value="ENSBIXG00000016234.1"/>
    </dbReference>
    <dbReference type="OMA" id="VYYCEQT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000365">
        <property type="entry name" value="If kappa light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021229011" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="16"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="157" mass="17380" checksum="8D71C602D2BA3380" modified="2019-09-18" version="1">MRFPAQLLGLLLLWVPGSSGDVVLTQTPLSLSVIPGETVSISCKSTQSLKYSDGKTYLYWLQHKPGQSPRQLIYQISNRNTGVPDRFTGSGAETDFTLTISSVQAEDVGVYYCFQATHDPPTVIHCSKCSFSGELTADALSLYKRQKGWNHAFLFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHJ0</accession>
    <name>A0A4W2CHJ0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nidogen-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Entactin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">NID1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with FBLN1. Interacts with LGALS3BP. Interacts with PLXDC1. Interacts with SVEP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHJ0"/>
    <dbReference type="SMR" id="A0A4W2CHJ0"/>
    <dbReference type="STRING" id="30522.A0A4W2CHJ0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005105.1">
        <property type="protein sequence ID" value="ENSBIXP00000005763.1"/>
        <property type="gene ID" value="ENSBIXG00000011903.1"/>
    </dbReference>
    <dbReference type="OMA" id="PGTGNQF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00255">
        <property type="entry name" value="nidG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000270">
        <property type="entry name" value="Nidogen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000281">
        <property type="entry name" value="Nidogen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000297">
        <property type="entry name" value="Nidogen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000030">
        <property type="entry name" value="Nidogen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.155.10:FF:000001">
        <property type="entry name" value="Nidogen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000666">
        <property type="entry name" value="nidogen-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000001">
        <property type="entry name" value="Testican-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.155.10">
        <property type="entry name" value="Green fluorescent protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006605">
        <property type="entry name" value="G2_nidogen/fibulin_G2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009017">
        <property type="entry name" value="GFP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF6">
        <property type="entry name" value="NIDOGEN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07474">
        <property type="entry name" value="G2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00682">
        <property type="entry name" value="G2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54511">
        <property type="entry name" value="GFP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50993">
        <property type="entry name" value="NIDOGEN_G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021262384" description="Nidogen-1" evidence="21">
        <location>
            <begin position="31"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="25">
        <location>
            <begin position="106"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Nidogen G2 beta-barrel" evidence="23">
        <location>
            <begin position="427"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="665"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="707"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="755"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="799"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="24">
        <location>
            <begin position="843"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="984"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="1027"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="1070"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="309"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="883"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1241" mass="136001" checksum="A9657FD189967B56" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI49</accession>
    <name>A0A4W2CI49_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="10">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="10">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="10">HAD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAAO</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the 3-HAO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CI49"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022884.1">
        <property type="protein sequence ID" value="ENSBIXP00000012763.1"/>
        <property type="gene ID" value="ENSBIXG00000017823.1"/>
    </dbReference>
    <dbReference type="OMA" id="MWLWQLE"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000077">
        <property type="entry name" value="3-hydroxyanthranilate 3,4-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Domain A (catalytic)" evidence="10">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Domain B" evidence="10">
        <location>
            <begin position="339"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="409" mass="46159" checksum="F663A3E980332FCF" modified="2019-09-18" version="1">MRDRSRRSFEFLKLSSLDSGVSSVPFRSATTTSPPGPLPTRAVFPRILSLEEREESRGPIGLPRLRTHTGKQSVSARSRGSRLAALTPGWNHQRLRRPIGGWAEARPFDRAEDTALECGSRETMERPVRVKAWVEENRGSFLPPVCNKLLHQKQLKIMFVGGPNTRKDYHIEEGEEVFYQLEGDMLLRVLERGKHRDVVIRQGEIFLLPAGVPHSPQRFANTVGLVIERRRLKTELDGLRYYVGDTTDVLFEKWFYCEDLGTQLAPIIQEFFSSEQYRTGKPNPDQLLKEPPFPLSTRSVMEPMCLEAWLDGHRKELQAGTPLSLFGDTYESQVMVHGQGSSEGLRRDVDVWLWQLEGSSVVTMEGQRLSLTLDDSLLVPAGTLYGWERGQGSVALSVTQDPACKKSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJP8</accession>
    <name>A0A4W2CJP8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113875553</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJP8"/>
    <dbReference type="STRING" id="30522.A0A4W2CJP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023643.1">
        <property type="protein sequence ID" value="ENSBIXP00000013300.1"/>
        <property type="gene ID" value="ENSBIXG00000000698.1"/>
    </dbReference>
    <dbReference type="OMA" id="FCVCPQM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF253">
        <property type="entry name" value="OLFACTORY RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="54"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="35646" checksum="EEF5B9B116D23D37" modified="2019-09-18" version="1">MSINFVSLQMERSLELGNMTRVQEFILLGLSISPETREVLFAVFLTLYLLTLLENALIVFLVCSHTELHKPMYFFLGNLSCLEMCYVSVTMPSLLVGLWTGPYHVPFTACMTQLFFFIILICTECTLLASMACDHYVAICRPLHYPLLMRPQVCLGLAGTSWLGGLLVSVSKTACIAGLSYCGPNVLNHFFCDVSPLLNLSCTHVALTELVDFLSAIVIFCGTLLVSLASYSAIGVAVLRMPSAAARRKDFSTCASHLVVVGIFYSAALFIYCRPSRIRSMDLNKLLSVVYTVATPMCNLVIYCLRNREVHAALLRTLRWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CM09</accession>
    <name>A0A4W2CM09_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RUN and FYVE domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">ARL8 effector that promotes the coupling of endolysosomes to dynein-dynactin for retrograde transport along microtubules. Acts by binding both GTP-bound ARL8 and dynein-dynactin. In nonneuronal cells, promotes concentration of endolysosomes in the juxtanuclear area. In hippocampal neurons, drives retrograde transport of endolysosomes from the axon to the soma. Positive regulator of macroautophagy in dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger. Positive regulator of osteosclast bone-resorbing activity, possibly by promoting late endosome-lysosome fusion by acting as an adapter protein between RAB7A on late endosomes and LAMP2 on primary lysosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CM09"/>
    <dbReference type="SMR" id="A0A4W2CM09"/>
    <dbReference type="STRING" id="30522.A0A4W2CM09"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024971.1">
        <property type="protein sequence ID" value="ENSBIXP00000014191.1"/>
        <property type="gene ID" value="ENSBIXG00000018941.1"/>
    </dbReference>
    <dbReference type="OMA" id="APGCCVG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071353">
        <property type="term" value="P:cellular response to interleukin-4"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051050">
        <property type="term" value="P:positive regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15745">
        <property type="entry name" value="FYVE_RUFY4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000015">
        <property type="entry name" value="RUN and FYVE domain containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042939">
        <property type="entry name" value="RUFY4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059036">
        <property type="entry name" value="RUFY4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47732">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47732:SF1">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25366">
        <property type="entry name" value="RUFY4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RUN" evidence="16">
        <location>
            <begin position="33"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="15">
        <location>
            <begin position="521"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="422"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="571" mass="64459" checksum="4BC84DEFCF888BD1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNJ0</accession>
    <name>A0A4W2CNJ0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATPase family gene 2 protein homolog B</fullName>
            <ecNumber evidence="3">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">AFG2 AAA ATPase homolog B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Ribosome biogenesis protein SPATA5L1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Spermatogenesis-associated protein 5-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">AFG2B</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity. Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of the 55LCC heterohexameric ATPase complex composed at least of AIRIM, AFG2A, AFG2B and CINP. Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the AAA ATPase family. AFG2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CNJ0"/>
    <dbReference type="STRING" id="30522.A0A4W2CNJ0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025704.1">
        <property type="protein sequence ID" value="ENSBIXP00000014694.1"/>
        <property type="gene ID" value="ENSBIXG00000019323.1"/>
    </dbReference>
    <dbReference type="OMA" id="DRHIYVA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000075">
        <property type="entry name" value="Spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF194">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="233"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="499"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00084032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="767" mass="82568" checksum="C2D0A2585CEE60E6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CP18</accession>
    <name>A0A4W2CP18_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ArfGAP with dual PH domains 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ADAP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CP18"/>
    <dbReference type="STRING" id="30522.A0A4W2CP18"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000448.1">
        <property type="protein sequence ID" value="ENSBIXP00000008086.1"/>
        <property type="gene ID" value="ENSBIXG00000013961.1"/>
    </dbReference>
    <dbReference type="OMA" id="YYNRNDA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08843">
        <property type="entry name" value="ArfGap_ADAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13252">
        <property type="entry name" value="PH1_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01251">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000011">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000080">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000099">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052589">
        <property type="entry name" value="Arf-GAP_dual-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037849">
        <property type="entry name" value="PH1_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037851">
        <property type="entry name" value="PH2_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021:SF5">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="7"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="129"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="252"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="374" mass="43335" checksum="B6EC8C98EBB0626E" modified="2019-09-18" version="1">MAKERRKAVLELLQRPGNARCADCGAPDPDWASYTLGVFICLSCSGIHRNIPHVSKVKSVRLDTWEDVQVEFMASRGNAIARATFESRVPPFYYRPSASDCPLLREQWIRAKYERQEFAHPERQEPYSAGYREGFLWKRGRDNGQFLSRKFVLTEREGALKYFNRSDAKEPKAVMKIEHLNATFQPAKIGHPHGLQVTYLKDNSTRNIFVYHEDGKEMVDWFNALRAARFHYLQVAFPGASNADLVPKLSRNYLQEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSRESGYTVLDGLPPSTQGHHWPHGITIVTPERRFLLACETESEQRAWMEALQKVVDRPMLPQEYAVEAHFKHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPC1</accession>
    <name>A0A4W2CPC1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CPC1"/>
    <dbReference type="STRING" id="30522.A0A4W2CPC1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026200.1">
        <property type="protein sequence ID" value="ENSBIXP00000015002.1"/>
        <property type="gene ID" value="ENSBIXG00000019592.1"/>
    </dbReference>
    <dbReference type="OMA" id="LTQQGPW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF13">
        <property type="entry name" value="LEUCINE-RICH REPEAT NEURONAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021466934" description="Fibronectin type-III domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="588"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="368"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="437"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="744" mass="79378" checksum="13514A197D0970BA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CR23</accession>
    <name>A0A4W2CR23_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Proprotein convertase subtilisin/kexin type 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Proprotein convertase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Proprotein convertase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Subtilisin/kexin-like protease PC5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive and regulated secretory pathways. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins. May be responsible for the maturation of gastrointestinal peptides. May be involved in the cellular proliferation of adrenal cortex via the activation of growth factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Endomembrane system</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23 24">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000027237.1">
        <property type="protein sequence ID" value="ENSBIXP00000015637.1"/>
        <property type="gene ID" value="ENSBIXG00000000469.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007565">
        <property type="term" value="P:female pregnancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000011">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000018">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000033">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000035">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000037">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000042">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000049">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000053">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000057">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000076">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000002">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000022">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF7">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 23 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0635">Pregnancy</keyword>
    <keyword evidence="4 23 24" id="KW-0645">Protease</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 23 24" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8 26" id="KW-0732">Signal</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021232774" description="Proprotein convertase subtilisin/kexin type 5" evidence="26">
        <location>
            <begin position="33"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="1744"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="27">
        <location>
            <begin position="461"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22 23">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22 23">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22 23">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1857" mass="206721" checksum="6BDD008CFC951F9D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CR26</accession>
    <name>A0A4W2CR26_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">TNF receptor-associated factor</fullName>
            <ecNumber evidence="21">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TNF receptor-associated factor family. A subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CR26"/>
    <dbReference type="STRING" id="30522.A0A4W2CR26"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025327.1">
        <property type="protein sequence ID" value="ENSBIXP00000014429.1"/>
        <property type="gene ID" value="ENSBIXG00000003382.1"/>
    </dbReference>
    <dbReference type="OMA" id="FMHLQAL"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070498">
        <property type="term" value="P:interleukin-1-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141124">
        <property type="term" value="P:intracellular signaling cassette"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031663">
        <property type="term" value="P:lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03776">
        <property type="entry name" value="MATH_TRAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16643">
        <property type="entry name" value="mRING-HC-C3HC3D_TRAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000010">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000179">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000211">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000289">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037309">
        <property type="entry name" value="TRAF6_MATH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027139">
        <property type="entry name" value="TRAF6_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041310">
        <property type="entry name" value="TRAF6_Z2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF152">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18048">
        <property type="entry name" value="TRAF6_Z2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 21" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="18" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="11 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16 21 22" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="26">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="26">
        <location>
            <begin position="205"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="25">
        <location>
            <begin position="351"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="22">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="22">
        <location>
            <begin position="205"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="300"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR015614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="523" mass="59563" checksum="2E77E6BF0FD3CFB9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CR76</accession>
    <name>A0A4W2CR76_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">OXR1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in protection from oxidative damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CR76"/>
    <dbReference type="SMR" id="A0A4W2CR76"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025380.1">
        <property type="protein sequence ID" value="ENSBIXP00000014479.1"/>
        <property type="gene ID" value="ENSBIXG00000007605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.350.10:FF:000002">
        <property type="entry name" value="Oxidation resistance protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF69">
        <property type="entry name" value="OXIDATION RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="7">
        <location>
            <begin position="98"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="711"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="872" mass="98112" checksum="D718451449979ED8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRB7</accession>
    <name>A0A4W2CRB7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRB7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025506.1">
        <property type="protein sequence ID" value="ENSBIXP00000014404.1"/>
        <property type="gene ID" value="ENSBIXG00000019111.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="178"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="450" mass="52089" checksum="3317CB55C7317BC3" modified="2019-09-18" version="1">MQKSKMAVWGGLTKAVKRREAKSKGEKERYKHLNAEFQRIARRDKKAFFSNQCKEREENNRMGKTRDLFKKIRDTKGTSHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITDLEPDILECEVQWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRLVFIPIPKKGSAKECSNYRTIALISHASEVMLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKERGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQTGKGVCQGCILSPCLFNFYAEYIMRNAGLEEAQAGIRIAGRNVNNLRYADDTTLMAESEEEFKSLLMKVKEENEKVGLKLHIQKTKIMASSPITSWEIDGETVETVSDFIFFGLQNHCRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1Y7</accession>
    <name>A0A4W2D1Y7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="4 14">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113900013</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.</text>
    </comment>
    <comment type="function">
        <text evidence="14">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1Y7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031106.1">
        <property type="protein sequence ID" value="ENSBIXP00000018208.1"/>
        <property type="gene ID" value="ENSBIXG00000022075.1"/>
    </dbReference>
    <dbReference type="OMA" id="NIPTHHV"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
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        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
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        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF12">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="15">
        <location>
            <begin position="26"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="288" mass="31948" checksum="658BA9BB0FC61163" modified="2019-09-18" version="1">MANGFTYEDYQDTAKWLLSHTKHPQVAVICGTGLGCLINRLTQAQTFDYSEIPSFPESTVPGHAGRLVFGILNGRACVMMQGRFHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASIPVSGHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2U4</accession>
    <name>A0A4W2D2U4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative treble-clef zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C11H2orf42</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2U4"/>
    <dbReference type="STRING" id="30522.A0A4W2D2U4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031625.1">
        <property type="protein sequence ID" value="ENSBIXP00000018527.1"/>
        <property type="gene ID" value="ENSBIXG00000022358.1"/>
    </dbReference>
    <dbReference type="OMA" id="FWMPSQL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026049">
        <property type="entry name" value="C2orf42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029269">
        <property type="entry name" value="Zf-tcix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13518:SF1">
        <property type="entry name" value="C2ORF42 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13518">
        <property type="entry name" value="PUTATIVE TREBLE-CLEF ZINC-FINGER C2ORF42 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14952">
        <property type="entry name" value="zf-tcix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative treble-clef zinc-finger" evidence="2">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="574" mass="64034" checksum="D5DB0F96730EAF7D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3A1</accession>
    <name>A0A4W2D3A1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3A1"/>
    <dbReference type="STRING" id="30522.A0A4W2D3A1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032414.1">
        <property type="protein sequence ID" value="ENSBIXP00000018673.1"/>
        <property type="gene ID" value="ENSBIXG00000022464.1"/>
    </dbReference>
    <dbReference type="OMA" id="MHIRAPA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000076">
        <property type="entry name" value="SPARC-related modular calcium binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000012">
        <property type="entry name" value="SPARC-related modular calcium binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000004">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000003">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF21">
        <property type="entry name" value="SPARC-RELATED MODULAR CALCIUM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16597">
        <property type="entry name" value="Thyroglob_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="15">
        <location>
            <begin position="208"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="14">
        <location>
            <begin position="261"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="14">
        <location>
            <begin position="387"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="520"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="41"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="326"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="593"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="298"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="425"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="655" mass="73239" checksum="0D27CA44759FBC2B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D502</accession>
    <name>A0A4W2D502_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tyrosine-protein phosphatase non-receptor type 13</fullName>
            <ecNumber evidence="5">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">PTPN13</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Tyrosine phosphatase which negatively regulates FAS-induced apoptosis and NGFR-mediated pro-apoptotic signaling. May regulate phosphoinositide 3-kinase (PI3K) signaling through dephosphorylation of PIK3R2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 30">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts (via the first PDZ domain) with PLEKHA1 and PLEKHA2. Interacts (via the second PDZ domain) with TNFRSF6 (Fas receptor) (via C-terminus). Interacts (via the second PDZ domain) with TRIP6 (via the third LIM domain and C-terminus). Interacts (via the third PDZ domain) with NGFR (via C-terminal SVP motif) and PKN2 (via C-terminus). Interacts (via the second or fourth PDZ domains) with PDLIM4 (via C-terminus only or via combined C-terminus and LIM domain, but not LIM domain only). Found in a complex with PDLIM4 and TRIP6. Interacts with PDLIM4; this interaction results in dephosphorylation of SRC 'Tyr-419' by this protein leading to its inactivation. Interacts with BRD7. Interacts with RAPGEF6. Interacts with ARHGAP29. Interacts with PIK3R2; dephosphorylates PIK3R2. Interacts with FBXL2. Interacts (via the FERM domain) with ENTR1. Found in a complex with ENTR1, PTPN13 and GIT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="5"/>
    <dbReference type="STRING" id="30522.A0A4W2D502"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035319.1">
        <property type="protein sequence ID" value="ENSBIXP00000020940.1"/>
        <property type="gene ID" value="ENSBIXG00000024165.1"/>
    </dbReference>
    <dbReference type="OMA" id="ESPPHTI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036312">
        <property type="term" value="F:phosphatidylinositol 3-kinase regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13187">
        <property type="entry name" value="FERM_C_PTPH13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17195">
        <property type="entry name" value="FERM_F1_PTPN13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23072">
        <property type="entry name" value="PDZ1_PTPN13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06792">
        <property type="entry name" value="PDZ2-PTPN13_FRMPD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06695">
        <property type="entry name" value="PDZ3_PTPN13_FRMPD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06696">
        <property type="entry name" value="PDZ4_PTPN13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06697">
        <property type="entry name" value="PDZ5_PTPN13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14597">
        <property type="entry name" value="PTPc-N13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000242">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000011">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000107">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000084">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000086">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000105">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000129">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000174">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000082">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000034">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011019">
        <property type="entry name" value="KIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052074">
        <property type="entry name" value="NonRcpt_TyrProt_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012153">
        <property type="entry name" value="PTPN13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46900">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46900:SF1">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16599">
        <property type="entry name" value="PTN13_u3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000933">
        <property type="entry name" value="Tyr-Ptase_nr13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00750">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51377">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11 21" id="KW-0175">Coiled coil</keyword>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="364" mass="39592" checksum="6E69C1CBF9BCCD4E" modified="2019-09-18" version="1">MVDYYEVLGVQRHASAEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDRYGKEGLNGGGGGGSHFDSPFEFGFTFRNPEDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSTFSGFPSFGGAFPSFDAGFSSFGSLGHGGLTAFSSSSAFGGSGMGNYKSISTSTKVVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGVADEDAFAEECLRRGQSALPAQPASSSARSLRPPRPAPPPRPAAHAAGEDEDAPGAFCAGWKELVGRSKQGMSGEALLVSKPGARMRTFAGRCTQSRAAGAWSLCRETRRRLLPNALLVGEGGSVPAAPWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6P3</accession>
    <name>A0A4W2D6P3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Stearoyl-CoA desaturase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SCD5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027400691.1">
        <property type="nucleotide sequence ID" value="XM_027544890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D6P3"/>
    <dbReference type="SMR" id="A0A4W2D6P3"/>
    <dbReference type="STRING" id="30522.A0A4W2D6P3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050026.1">
        <property type="protein sequence ID" value="ENSBIXP00000021527.1"/>
        <property type="gene ID" value="ENSBIXG00000007406.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048870.1">
        <property type="protein sequence ID" value="ENSBIXP00005038926.1"/>
        <property type="gene ID" value="ENSBIXG00005003574.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894450"/>
    <dbReference type="CTD" id="79966"/>
    <dbReference type="GeneTree" id="ENSGT00940000162090"/>
    <dbReference type="OMA" id="CQHGPID"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF100">
        <property type="entry name" value="STEAROYL-COA DESATURASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="17">
        <location>
            <begin position="81"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="335" mass="38215" checksum="32F8B2C60533AB31" modified="2019-09-18" version="1">MPGPAVDAEKVPFRSAKEEIRAGVGVEGSEGGGGGGGRERPGARGHRQDIVWRNVFLMSLLHLAAVYSLVLIPKAQPLTLLWAYFCFLLTALGVTAGAHRLWSHRSYKAKLPLRIFLAAANSMAFQNDIFEWSRDHRVHHKYSETDADPHNARRGFFFSHIGWLFVRKHRDVIEKGRKLDVTDLLADPVVRFQRKYYKITVVLMCFVVPTLVPWYIWGESLWNSYFLASILRYTISLNVTWLVNSVAHMYGNRPYDKHISPRQNPLVTLGAIGEGFHNYHHTFPFDYSASEFGLNFNPTTWFIDFMCWLGLATDRKRATKQMIEARKARTGDGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7U0</accession>
    <name>A0A4W2D7U0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7U0"/>
    <dbReference type="STRING" id="30522.A0A4W2D7U0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034353.1">
        <property type="protein sequence ID" value="ENSBIXP00000020336.1"/>
        <property type="gene ID" value="ENSBIXG00000023664.1"/>
    </dbReference>
    <dbReference type="OMA" id="GASHYNI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000007">
        <property type="entry name" value="Acidic mammalian chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF388">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="6">
        <location>
            <begin position="30"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="361" mass="40789" checksum="FF8428ABDA4FCD53" modified="2019-09-18" version="1">MGTQETGKSMSFGARLPGSSVGTISICSAYKLVCYFTNWSQYRPEPAKFFPRDVDPCLCTHLIYAFATMNDNKIAPYEWNDIDVLYPGFQALKEHNKDLVTLLVVGGWNFGTQKFTTMVSSAANRRIFICSVIDFLRQHEFDGIDLDIEYPGSRGKAEEAGKPRLLITAAVSAGKETTDAGYEIAEIGKLLDFISMMTYDFHGGWDPCTGHNSPLHVGSKDQGNMCYFNCEYAMKYWRDNGVPSEKLIMGFPTYGRTFRLRSSGPYTREAGFWAYYEICTFLSEATNAWIEDQKVPYAYKDTEWVGYDNIRSYKYKVDFLKENNFGGAMVWAIDLDDFLGSFCNEDNYKKMGSGLPCLSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9U0</accession>
    <name>A0A4W2D9U0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SULT1E1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4-ethylphenol + 3'-phosphoadenylyl sulfate = 4-ethylphenyl sulfate + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49584"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:133681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:70608"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9U0"/>
    <dbReference type="SMR" id="A0A4W2D9U0"/>
    <dbReference type="STRING" id="30522.A0A4W2D9U0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038016.1">
        <property type="protein sequence ID" value="ENSBIXP00000022742.1"/>
        <property type="gene ID" value="ENSBIXG00000025535.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004062">
        <property type="term" value="F:aryl sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="38"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="295" mass="35140" checksum="B7CAE4FCF8620F6F" modified="2019-09-18" version="1">MDSKLDIFRRELVDVQGIPLFWSIVEEWSQVESFENRPDDLLIATYPKSGTTWISEILDLIYNHGDVEKCKRDAIYKRVPFMELIIPRLTNGVEDLNDMQSPRLVKKHLPVQLLPSSFWKNNCKMVYVARNANDVAVSYYYFYQMAKMHPDPGTWEEFLDKFMTGKVAFGSWYDHVKGWWEKKKDYCKLYLFYEDMKQDPKCEIQKLLKFLDKDLPEETVDKILYHSSFDMMKQNPSANYTTMPKFCMDHSVSPFMRKGVSGDWKNQFTVVQYERFEEDYEKKMKGSTLQFHSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA61</accession>
    <name>A0A4W2DA61_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA61"/>
    <dbReference type="STRING" id="30522.A0A4W2DA61"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035505.1">
        <property type="protein sequence ID" value="ENSBIXP00000021064.1"/>
        <property type="gene ID" value="ENSBIXG00000004871.1"/>
    </dbReference>
    <dbReference type="OMA" id="SIPHAFK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045100">
        <property type="entry name" value="TUT4/7_NTP_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19088">
        <property type="entry name" value="TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="2">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="168" mass="19529" checksum="CC2C3243E975C369" modified="2019-09-18" version="1">MEAGSPENKKPRSRPRKPRRTRNDENEQDGDLEGPVIDESVLSTKELLGLQQAEERLKRDCIDRLKRRPRNYPTAKYTCKLCAVLIESIAFAHKHIKEKRHKKNIKEKHEEELLTTLPPPTPSQINAIGIAIDKVVQEFGLHNENLEQRLEIKRIMENVFQHKLPGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDI0</accession>
    <name>A0A4W2DDI0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC-type containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ZCCHC3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027414704.1">
        <property type="nucleotide sequence ID" value="XM_027558903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DDI0"/>
    <dbReference type="STRING" id="30522.A0A4W2DDI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028956.1">
        <property type="protein sequence ID" value="ENSBIXP00000016830.1"/>
        <property type="gene ID" value="ENSBIXG00000020905.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051478.1">
        <property type="protein sequence ID" value="ENSBIXP00005042183.1"/>
        <property type="gene ID" value="ENSBIXG00005025484.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903159"/>
    <dbReference type="CTD" id="85364"/>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="OMA" id="QGDESAC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071360">
        <property type="term" value="P:cellular response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009597">
        <property type="term" value="P:detection of virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900246">
        <property type="term" value="P:positive regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032481">
        <property type="term" value="P:positive regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057810">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF4">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23057">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23058">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="404" mass="43683" checksum="B9C9FA325D3ACEE7" modified="2019-09-18" version="1">MATGGGAEEERKRGRPQLLGPARPSTRAEEAEGGREKMGWAQVVKNLAEKKGEFRESRPPRREEESGSGAGGGLGAPAGLATPSLGDFPPAGRGDPKGRRRDPAGEAADSRKKKGAAEAGRRKKAEAAAMTTPARPDAAEDAADRPPQDEQATAAGSAAGPGKGRFLVRICFQGDEGACPTRDFVVGALILRSIGMDPSDIYAVIQIPGSREFDVSFRSAEKLALFLRVYEEKREQEDCWENFVVLGRSKSSLKTLFILFRNETVDVEDIVTWLKRHCDVLAVPVKVTDRFGIWTGEYKCEIELRQGEGGVRHLPGAFFLGAERGYSWYKGQPKTCFKCGSRTHMSGSCTQDRCFRCGEEGHLSPYCRKGIVCNLCGKRGHAFAQCPKAVHNSVGAQLTGVAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DL96</accession>
    <name>A0A4W2DL96_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coiled-coil-helix-coiled-coil-helix domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CHCHD2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027383023.1">
        <property type="nucleotide sequence ID" value="XM_027527222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DL96"/>
    <dbReference type="SMR" id="A0A4W2DL96"/>
    <dbReference type="STRING" id="30522.A0A4W2DL96"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014145.1">
        <property type="protein sequence ID" value="ENSBIXP00000027203.1"/>
        <property type="gene ID" value="ENSBIXG00000028628.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041507.1">
        <property type="protein sequence ID" value="ENSBIXP00005025662.1"/>
        <property type="gene ID" value="ENSBIXG00005004840.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883475"/>
    <dbReference type="CTD" id="51142"/>
    <dbReference type="GeneTree" id="ENSGT00440000038159"/>
    <dbReference type="OMA" id="CDADARN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905448">
        <property type="term" value="P:positive regulation of mitochondrial ATP synthesis coupled electron transport"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of cellular response to hypoxia"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF3">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="153" mass="15634" checksum="4B1D45AD63BD26D4" modified="2019-09-18" version="1">MPRGSRSRTSRVAPPASRAPQMRAAPRPAPAAQPPAMAPPSAVGSPAAAPRQPGLMAQMATTAAGVAVGSAVGHTLGHAITGGFSGGSSAEPSRPDITYQEPQGTQPAQLQQNGPCFYEVKQFLECAQSQGDLKLCEGFSEVLKQCRLANGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQ72</accession>
    <name>A0A4W2DQ72_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 43S pre-initiation complex (43S PIC), which is composed of the 40S ribosomal subunit, EIF1, eIF1A (EIF1AX), eIF3 complex, EIF5 and eIF2-GTP-initiator tRNA complex (eIF2 ternary complex). Interacts with EIF5; this interaction contributes to the maintenance of EIF1 within the open 43S PIC. Interacts through its C-terminal domain (CTD) with the CTD of EIF5B; from the location of the start codon by the 43S complex until the formation of the 80S complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQ72"/>
    <dbReference type="STRING" id="30522.A0A4W2DQ72"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053002.1">
        <property type="protein sequence ID" value="ENSBIXP00000026607.1"/>
        <property type="gene ID" value="ENSBIXG00000029744.1"/>
    </dbReference>
    <dbReference type="OMA" id="INENTWE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="11">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="143" mass="16400" checksum="EB9987C2507B0C25" modified="2019-09-18" version="1">MPKNKGKGGRNRRKGKKENEYEKRDLVFKEDGQEYAQVTKMLGNARLEAMCFDGVTRICHIRGSLRKKVWINTSDIILIGLRDYQDNKADVILKYNADEARSLKAYGELPEYAELSETDTFDAEDDDEIQFKDPAGEDEDMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQE5</accession>
    <name>A0A4W2DQE5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 4E family member 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">EIF4E1B</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQE5"/>
    <dbReference type="STRING" id="30522.A0A4W2DQE5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049825.1">
        <property type="protein sequence ID" value="ENSBIXP00000021184.1"/>
        <property type="gene ID" value="ENSBIXG00000004903.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034079.1">
        <property type="protein sequence ID" value="ENSBIXP00005020642.1"/>
        <property type="gene ID" value="ENSBIXG00005023696.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160838"/>
    <dbReference type="OMA" id="PKWEDET"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="132" mass="14868" checksum="C6FA2F8D9211C4D2" modified="2019-09-18" version="1">SSGCDYALFKDGIKPMWEDSRNKRGGRWLVSLTKQQRHIELDHLWLETLLCLIGESFGEHSQEVCGAVINIRAKGDKIAVWTREAENQEGVLHIGRVYKERLGLSTKTIIGYQAHADTATKSNSLAKNKFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DR01</accession>
    <name>A0A4W2DR01_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Syntaxin-1A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DR01"/>
    <dbReference type="STRING" id="30522.A0A4W2DR01"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015096.1">
        <property type="protein sequence ID" value="ENSBIXP00000026916.1"/>
        <property type="gene ID" value="ENSBIXG00000029163.1"/>
    </dbReference>
    <dbReference type="OMA" id="KTTHGPK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15880">
        <property type="entry name" value="SNARE_syntaxin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000042">
        <property type="entry name" value="Syntaxin 11b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF84">
        <property type="entry name" value="SYNTAXIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="249"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="125"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="373" mass="41573" checksum="6DEADC2EDA074AD7" modified="2019-09-18" version="1">MLQVSLRLGAEGTARPCPCQGDRQGWPGLGAPTEDPAIGGSWAAWGPRTHGTQWSYHRGACVSTPLQAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKISENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQALSSQNPSSGPLAAWRGPSGAGVPLALQGEMIDRIEYNVEHSVDYVERAVSDTKKAVKYQSKARRKKIMIVICCVVLGIVIASTFGGIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSW9</accession>
    <name>A0A4W2DSW9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM26</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSW9"/>
    <dbReference type="STRING" id="30522.A0A4W2DSW9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006404.1">
        <property type="protein sequence ID" value="ENSBIXP00000029673.1"/>
        <property type="gene ID" value="ENSBIXG00000000873.1"/>
    </dbReference>
    <dbReference type="OMA" id="HHGTQYC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019169">
        <property type="entry name" value="Transmembrane_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168">
        <property type="entry name" value="TMEM26 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09772">
        <property type="entry name" value="Tmem26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="368" mass="41680" checksum="89EE24907687F51A" modified="2019-09-18" version="1">MEGLVLLNALATRLLFVLHSLVGVWRVTVVKKEPWYWLLALLNLLLFLETALTLKFKRGRGYKWFSPAIFLYLISIVPSLWLLEMHHETQYCSSQFGGLSQNTSRKENFNQTLLSAEHTNRADDLIATAKVFVSNLSTVCEKVWTLGLHQTFLLMLIIGRWLLPIGSGITRDQLSQLLLMFVGTAADILEFTSETLEEENVRNSPALVYSILVIWTWSMLQFPLDLAVQNVVCPVSMTTRGFSSRFFCQYSADLWNIGISIFIQDGPFLVVRLILMTYFEVINQMLVFFAAKNFLVVVLQFYRLVVLALDVRASLRSQRERLKGEHRCPDVPAECGVSPEAWPGSSMEALAIPLQASPVTSDDSHPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTL5</accession>
    <name>A0A4W2DTL5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Exocyst complex component 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Exocyst complex component Sec3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">EXOC1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EEF1A1. Interacts with SLC6A9; interaction increases the transporter capacity of SLC6A9 probably by promoting its insertion into the cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
            <location evidence="3">Midbody ring</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SEC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTL5"/>
    <dbReference type="STRING" id="30522.A0A4W2DTL5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037484.1">
        <property type="protein sequence ID" value="ENSBIXP00000022395.1"/>
        <property type="gene ID" value="ENSBIXG00000025267.1"/>
    </dbReference>
    <dbReference type="OMA" id="NQHVMSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090543">
        <property type="term" value="C:Flemming body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14683">
        <property type="entry name" value="PH-EXOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.90:FF:000001">
        <property type="entry name" value="exocyst complex component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF33">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="19">
        <location>
            <begin position="31"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="486"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="580"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="214"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="928" mass="106005" checksum="A0791A3156690605" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DVS5</accession>
    <name>A0A4W2DVS5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Purinergic receptor P2Y12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">P2RY12</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DVS5"/>
    <dbReference type="SMR" id="A0A4W2DVS5"/>
    <dbReference type="STRING" id="30522.A0A4W2DVS5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050892.1">
        <property type="protein sequence ID" value="ENSBIXP00000023294.1"/>
        <property type="gene ID" value="ENSBIXG00000025787.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010285.1">
        <property type="protein sequence ID" value="ENSBIXP00005003452.1"/>
        <property type="gene ID" value="ENSBIXG00005000542.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267167"/>
    <dbReference type="OMA" id="FVCQVTQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044298">
        <property type="term" value="C:cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031253">
        <property type="term" value="C:cell projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001621">
        <property type="term" value="F:G protein-coupled ADP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071318">
        <property type="term" value="P:cellular response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043270">
        <property type="term" value="P:positive regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904139">
        <property type="term" value="P:regulation of microglial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006930">
        <property type="term" value="P:substrate-dependent cell migration, cell extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150063">
        <property type="term" value="P:visual system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15150">
        <property type="entry name" value="7tmA_P2Y12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000049">
        <property type="entry name" value="G-protein coupled receptor 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005394">
        <property type="entry name" value="P2Y12_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF0">
        <property type="entry name" value="P2Y PURINOCEPTOR 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01569">
        <property type="entry name" value="P2Y12PRNCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="42"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023294.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005003452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="342" mass="39263" checksum="1484784EB9E7CA03" modified="2019-09-18" version="1">TRAMDNLTSVAGNSSQCSRDYKITQVLFPLLYTVLFFVGLITNSLAMRIFFQIRSKSNFIIFLKNTVISDLLMILTFPFKILSDTKLGTGPLRAFVCQVTSVVFYFTMYISISFLGLITIDRYQKTTRPFKTANPNNLLGAKILSVVIWAFMFLISLPNMILTNRRPSDKTVKKCSFLKSEFGLVWHEIVNYVCQVIFWINFLIVIVCYTLITKELYKSYVRTRGAGKVPKKKVNIKVFIIIAVFFICFVPFHFARIPYTLSQTRDVFDCTAENTLFYVKESTLWLTSLNACLDPFIYFFLCKSFKNSLMSMLRCSNSTSPPSHDNRKKGQDGSDPSEETPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXN0</accession>
    <name>A0A4W2DXN0_BOBOX</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSBIXG00005015217.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="135" mass="13990" checksum="3FE2F5E4598698CF" modified="2025-10-08" version="2">MGFTGPHAPSLGGAEHRSAVYSSSVSTNRLDTAQMAMIRYTFTSTNAVSAPAAPDNAREEALGAWPLSSSDSNTVAVAVSAGSPWSFTSTSRRWRGASASSRARVVLISPVYSPTRNGLRAPPAAGCSSYDSHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZ36</accession>
    <name>A0A4W2DZ36_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="FAM222"/>
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        <property type="entry name" value="PROTEIN FAM222A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN FAM222A-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="FAM222A"/>
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    </dbReference>
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            <begin position="219"/>
            <end position="262"/>
        </location>
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        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="460" mass="47126" checksum="1E1CDD0AC4DD2975" modified="2019-09-18" version="1">MLACLQRTQNAPGQHLACPTKSLELRKCETVASSMHSSRYPSPAELDAYAEKVANSPLSIKIFPTNIRVPQHKHLSRTVNGYDTSGQRYSPYPPHTAGYQGLLAIVKAAVSSSSSAAAPAGPAKSVLKSAEGKRTKLSPAAVQVGVAPYPAPSTLGPLAYPKPPEVPAPPPGLPAAAAPAAAVIPLPGRGLPLPPSNLPSIHSLLYQLHQQCQAPGAAPAACQAVPGPPPSPAKHGPVPGFPRTAYSATAGPPECRKGAELGQGSTPAALTLAGAAKPAGYADGGLDYLLWPQKPPPPPPPPPQPLRAYGGGALASKSPEACGGRAYERASGSPLNCGVGLPASFTVGQYFAAPWNSVLVTPTSDCYNPAAAAAAAAAELGPGAARELAGPPAETLSGLPSKSVCNTAVLSSSLQSLEYLINDIRPPCIKERMLGKGYETVAVPRLLDHQHAHIRLPVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0S7</accession>
    <name>A0A4W2E0S7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Semaphorin 3D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SEMA3D</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395643.1">
        <property type="nucleotide sequence ID" value="XM_027539842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E0S7"/>
    <dbReference type="SMR" id="A0A4W2E0S7"/>
    <dbReference type="STRING" id="30522.A0A4W2E0S7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041740.1">
        <property type="protein sequence ID" value="ENSBIXP00000025197.1"/>
        <property type="gene ID" value="ENSBIXG00000006756.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031805.1">
        <property type="protein sequence ID" value="ENSBIXP00005039906.1"/>
        <property type="gene ID" value="ENSBIXG00005003818.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891596"/>
    <dbReference type="CTD" id="223117"/>
    <dbReference type="GeneTree" id="ENSGT00940000156681"/>
    <dbReference type="OMA" id="KIYWPAT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11252">
        <property type="entry name" value="Sema_3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041416">
        <property type="entry name" value="IL-1RAcP-like_ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042582">
        <property type="entry name" value="Sema3D_Sema"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="39 40" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="39" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="30">Inhibits angiogenesis in a CD36-dependent manner.</text>
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        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
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        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
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            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
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            <location evidence="1">Cell projection</location>
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            <location evidence="29">Postsynaptic density</location>
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        <subcellularLocation>
            <location evidence="4">Secreted</location>
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    <comment type="similarity">
        <text evidence="6">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
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        <property type="match status" value="5"/>
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    <keyword evidence="12 34" id="KW-0812">Transmembrane</keyword>
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    <feature type="signal peptide" evidence="35">
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    <feature type="chain" id="PRO_5021252650" description="Adhesion G protein-coupled receptor B1" evidence="35">
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            <begin position="28"/>
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    <feature type="transmembrane region" description="Helical" evidence="34">
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            <begin position="941"/>
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    <feature type="transmembrane region" description="Helical" evidence="34">
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            <begin position="974"/>
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    <feature type="transmembrane region" description="Helical" evidence="34">
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            <begin position="1005"/>
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    <feature type="transmembrane region" description="Helical" evidence="34">
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    <feature type="transmembrane region" description="Helical" evidence="34">
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    <feature type="transmembrane region" description="Helical" evidence="34">
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            <begin position="1155"/>
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        <location>
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            <end position="931"/>
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    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="38">
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            <begin position="939"/>
            <end position="1180"/>
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    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="310"/>
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    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
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        <location>
            <begin position="314"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="33">
        <location>
            <begin position="1382"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="33">
        <location>
            <begin position="1431"/>
            <end position="1451"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="32">
        <source>
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    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000256" key="35">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1457" mass="158956" checksum="CF57AFA818A1D5C0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5D8</accession>
    <name>A0A4W2E5D8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5D8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022394.1">
        <property type="protein sequence ID" value="ENSBIXP00000034535.1"/>
        <property type="gene ID" value="ENSBIXG00000017540.1"/>
    </dbReference>
    <dbReference type="OMA" id="QQWARIP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="43016" checksum="7A6F7B85789BD2F4" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYSKAFDCVDHKKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIRKGVRQGYILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITTDGDCSHEIERRLLLGRKVMTNLDSIFKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWYWRRLLRVPWTTRRSNQSILKEISPGISLEGMMLKLKLQYFGHLMRRIDSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELLELVMDREAWCAAIHGVAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E6G9</accession>
    <name>A0A4W2E6G9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bardet-Biedl syndrome 2 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BBS2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
            <location evidence="6">Microtubule organizing center</location>
            <location evidence="6">Centrosome</location>
            <location evidence="6">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027372309.1">
        <property type="nucleotide sequence ID" value="XM_027516508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E6G9"/>
    <dbReference type="STRING" id="30522.A0A4W2E6G9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044000.1">
        <property type="protein sequence ID" value="ENSBIXP00000032365.1"/>
        <property type="gene ID" value="ENSBIXG00000014921.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019765.1">
        <property type="protein sequence ID" value="ENSBIXP00005034600.1"/>
        <property type="gene ID" value="ENSBIXG00005015601.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876828"/>
    <dbReference type="CTD" id="583"/>
    <dbReference type="GeneTree" id="ENSGT00390000017113"/>
    <dbReference type="OMA" id="MSDGANC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030534">
        <property type="term" value="P:adult behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014824">
        <property type="term" value="P:artery smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048854">
        <property type="term" value="P:brain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021766">
        <property type="term" value="P:hippocampus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038108">
        <property type="term" value="P:negative regulation of appetite by leptin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040015">
        <property type="term" value="P:negative regulation of multicellular organism growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040018">
        <property type="term" value="P:positive regulation of multicellular organism growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060296">
        <property type="term" value="P:regulation of cilium beat frequency involved in ciliary motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021756">
        <property type="term" value="P:striatum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000967">
        <property type="entry name" value="Bardet-Biedl syndrome 2 protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016616">
        <property type="entry name" value="Bardet-Biedl_syndrome_2_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055381">
        <property type="entry name" value="BBS2_CtH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029333">
        <property type="entry name" value="BBS2_GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055380">
        <property type="entry name" value="BBS2_hp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029429">
        <property type="entry name" value="BBS2_Mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029430">
        <property type="entry name" value="BBS2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055379">
        <property type="entry name" value="BBS2_pf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32465">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32465:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23351">
        <property type="entry name" value="BBS2_CtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14782">
        <property type="entry name" value="BBS2_GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23353">
        <property type="entry name" value="BBS2_hp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14783">
        <property type="entry name" value="BBS2_Mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14781">
        <property type="entry name" value="BBS2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23350">
        <property type="entry name" value="BBS2_pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013684">
        <property type="entry name" value="BBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 6" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary BBSome complex subunit 2 N-terminal" evidence="8">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ciliary BBSome complex subunit 2 middle region" evidence="10">
        <location>
            <begin position="165"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 GAE" evidence="9">
        <location>
            <begin position="396"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 platform" evidence="11">
        <location>
            <begin position="484"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 hairpin" evidence="13">
        <location>
            <begin position="587"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 C-terminal helix bundle" evidence="12">
        <location>
            <begin position="689"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060342"/>
        </source>
    </evidence>
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        <source>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBD5"/>
    <dbReference type="STRING" id="30522.A0A4W2EBD5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045433.1">
        <property type="protein sequence ID" value="ENSBIXP00000034198.1"/>
        <property type="gene ID" value="ENSBIXG00000017127.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005024169.1">
        <property type="protein sequence ID" value="ENSBIXP00005036497.1"/>
        <property type="gene ID" value="ENSBIXG00005018064.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165608"/>
    <dbReference type="OMA" id="KIRCKSE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004137">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002278">
        <property type="entry name" value="Zinc finger protein 320"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000133">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001700">
        <property type="entry name" value="Zinc finger protein 677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="444" mass="51420" checksum="FB2157FFCC7C8162" modified="2019-09-18" version="1">MLSLSGYMWIYNKLKPKANSDTGVFQRVMLGRYKSQEVRDFYFTEIQENMHVFECQWTDDEGNDKGMPVNNNENLTDGKNQHGRRDPGNKPIENKLALSFQDEWCIFKREEKIDEFNEADKTSNSSTSFLPLQTISPIVQTNISNPYGNDFIHPSVLTQDQKAYKEKPYKCNECDKTFPYSSNLSRHQLIHTGEKPYNCDVCGKVFSRNSHLACHQRIHTGEKPYKCKECGKTFNQCSNLTRHHHQTVHFGEKSYKCNECGKAFRKRSSLTQHKRIHTGEKPYRCNECGKAFRKHSNLTQHKVVHTGEKPYRCNECGKAFSVHSALRNHQIIHTGEKPYKCDVCGKVFSRNSILASHQRLHTGEKPYKCKECGKSFMQRSLLWIHEKIHTGEKPYKCNECGKAFPVRSILTQHMRIHTGEKPYICTECEKSFKTYVCGKACNCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EC30</accession>
    <name>A0A4W2EC30_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Atypical kinase COQ8A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chaperone activity of bc1 complex-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Coenzyme Q protein 8A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">aarF domain-containing protein kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">COQ8A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is still unclear: may act as a protein kinase that mediates phosphorylation of COQ3. According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates. However, the small molecule kinase activity was not confirmed by another publication. Shows an unusual selectivity for binding ADP over ATP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EC30"/>
    <dbReference type="SMR" id="A0A4W2EC30"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028274.1">
        <property type="protein sequence ID" value="ENSBIXP00000037056.1"/>
        <property type="gene ID" value="ENSBIXG00000020577.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF1">
        <property type="entry name" value="ATYPICAL KINASE COQ8A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="20">
        <location>
            <begin position="300"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="648" mass="72082" checksum="217518E10C95C73D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EF74</accession>
    <name>A0A4W2EF74_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import receptor subunit TOM22 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Translocase of outer membrane 22 kDa subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TOMM22</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396570.1">
        <property type="nucleotide sequence ID" value="XM_027540769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF74"/>
    <dbReference type="SMR" id="A0A4W2EF74"/>
    <dbReference type="STRING" id="30522.A0A4W2EF74"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003613.1">
        <property type="protein sequence ID" value="ENSBIXP00000030586.1"/>
        <property type="gene ID" value="ENSBIXG00000012608.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040228.1">
        <property type="protein sequence ID" value="ENSBIXP00005043625.1"/>
        <property type="gene ID" value="ENSBIXG00005004713.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892221"/>
    <dbReference type="CTD" id="56993"/>
    <dbReference type="GeneTree" id="ENSGT00390000016475"/>
    <dbReference type="OMA" id="DKDSGME"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15292" checksum="36FEC51220BD3680" modified="2019-09-18" version="1">MAAAAAGPGAPLSADELLPKGDAEKPEEELEEEDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPSLPGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFR9</accession>
    <name>A0A4W2EFR9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 835</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF835</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFR9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045980.1">
        <property type="protein sequence ID" value="ENSBIXP00000035074.1"/>
        <property type="gene ID" value="ENSBIXG00000000700.1"/>
    </dbReference>
    <dbReference type="OMA" id="PKKPWKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000111">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000380">
        <property type="entry name" value="zinc finger protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001011">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF366">
        <property type="entry name" value="ZINC FINGER PROTEIN ZFP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="85"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="94"/>
            <end position="132"/>
        </location>
    </feature>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 14">Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all. Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling. Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form. Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC. Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with MEFV.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
            <location evidence="14">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Secreted</location>
            <location evidence="2 14">Extracellular exosome</location>
        </subcellularLocation>
        <text evidence="14">The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">IL1B production occurs in 2 steps, each being controlled by different stimuli. First, inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1B (priming). Then additional signals are required for inflammasome assembly, leading to CASP1 activation, pro-IL1B processing and eventually secretion of the active cytokine. IL1B processing and secretion are temporarily associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the IL-1 family.</text>
    </comment>
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        <property type="entry name" value="IL-1_fam"/>
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        <property type="entry name" value="INTERLEUKIN-1 FAMILY MEMBER"/>
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        <property type="entry name" value="IL1HBGF"/>
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        <property type="entry name" value="INTERLEUKIN1"/>
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        <property type="entry name" value="INTRLEUKIN1B"/>
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        <property type="entry name" value="INTRLEUKN1AB"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INTERLEUKIN_1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0202">Cytokine</keyword>
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    <keyword evidence="7 13" id="KW-0666">Pyrogen</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Interleukin-1 propeptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="266" mass="30702" checksum="F43A6ABD18B1D975" modified="2019-09-18" version="1">MATVPEPINEMMAYYSDENELLFEADGPKQMKSCIQHLDLGSMGDGNIQLQISHQFYNKSFRQVVSVIVAMEKLRNSAYAHVFHDDDLRSILSFIFEEEPVIFETSSDEFLCDAPVQSIKCKLQDREQKSLVLASPCVLKALHLLSQEMNREVVFCMSFVQGEERDNKIPVALGIKDKNLYLSCVKKGDTPTLQLEEVDPKVYPKRNMEKRFVFYKTEIKNTVEFESVLYPNWYISTSQIEERPVFLGHFRGGQDITDFRMETLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2END5</accession>
    <name>A0A4W2END5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">POPDC1-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the popeye family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2END5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031049.1">
        <property type="protein sequence ID" value="ENSBIXP00000038261.1"/>
        <property type="gene ID" value="ENSBIXG00000004269.1"/>
    </dbReference>
    <dbReference type="OMA" id="FLCLTLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051146">
        <property type="term" value="P:striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006916">
        <property type="entry name" value="POPDC1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055272">
        <property type="entry name" value="POPDC1-3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101">
        <property type="entry name" value="POPEYE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101:SF15">
        <property type="entry name" value="POPEYE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04831">
        <property type="entry name" value="POPDC1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="POPDC1-3" evidence="8">
        <location>
            <begin position="26"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="364" mass="40731" checksum="BF0607CD90E65F1D" modified="2019-09-18" version="1">MSSNRSMEGQLLWHGPLCIAWRQDLEGAVFHLANCFLLLGFMGGSGVYGCFYLFGFLGTGYLCCVLWGWFGACGLDIVLWSVLLAMACVLQLAHLVYRLREDTLPEDLELLYKTLCLPLQVPLPAYKEIVHCCEEQVLTLATEQTYAVEGETPIDRLSLLLSGRVRVSQDGQFLHYIFPYQFMDSPEWESLHPSEEGVFQVTLTAETECSYISWPRKNLHLLLTKERYISRLFSVLLGYDISEKLYALNDKLFAKFGLRFDIRLPSLYHVLGSAAPDAGPQSEKDDEEVREPAATPPQALHTSVQQTPPSSPPPLVTSSPAPPPWVRMSRPDSGVLASRTPLRSYSEVMSRGQAPLAPTQTPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPI9</accession>
    <name>A0A4W2EPI9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPI9"/>
    <dbReference type="STRING" id="30522.A0A4W2EPI9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032300.1">
        <property type="protein sequence ID" value="ENSBIXP00000038789.1"/>
        <property type="gene ID" value="ENSBIXG00000004418.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049101.1">
        <property type="protein sequence ID" value="ENSBIXP00005039232.1"/>
        <property type="gene ID" value="ENSBIXG00005021582.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006813"/>
    <dbReference type="OMA" id="GHDGIRF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18490" checksum="156AF95F21D6A228" modified="2019-09-18" version="1">SNSMPHLSFIRSYQNASCRLSLWQLSTSTNLKDILADVIPKEQTRVKAFRQQHGKTVVGQITGHDGIRFRGYSIPECQKLLPKAKGGEEPLPEGLFWLLVTGQIPTEEQVSWLSQEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYSEGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESY6</accession>
    <name>A0A4W2ESY6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 782</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF782</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESY6"/>
    <dbReference type="STRING" id="30522.A0A4W2ESY6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035165.1">
        <property type="protein sequence ID" value="ENSBIXP00000040020.1"/>
        <property type="gene ID" value="ENSBIXG00000024059.1"/>
    </dbReference>
    <dbReference type="OMA" id="TSCLIQP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000784">
        <property type="entry name" value="Zinc finger protein 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000848">
        <property type="entry name" value="Zinc finger protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000829">
        <property type="entry name" value="zinc finger protein 510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000830">
        <property type="entry name" value="zinc finger protein 596 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="649"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="677"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="702" mass="81313" checksum="2FBE2F50F99BEDBD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETQ5</accession>
    <name>A0A4W2ETQ5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="30">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PRKAB2</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Acts as a Baeyer-Villiger monooxygenase on a broad range of substrates. Catalyzes the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Active on diverse carbonyl compounds, whereas soft nucleophiles are mostly non- or poorly reactive. In contrast with other forms of FMO it is non- or poorly active on 'classical' substrates such as drugs, pesticides, and dietary components containing soft nucleophilic heteroatoms. Able to oxidize drug molecules bearing a carbonyl group on an aliphatic chain, such as nabumetone and pentoxifylline. Also, in the absence of substrates, shows slow but yet significant NADPH oxidase activity. Acts as a positive modulator of cholesterol biosynthesis as well as glucose homeostasis, promoting metabolic aging via pleiotropic effects.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(2E)-geranial + NADPH + O2 + H(+) = (1E)-2,6-dimethylhepta-1,5-dien-1-yl formate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138375"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>N,N-dimethylaniline + NADPH + O2 + H(+) = N,N-dimethylaniline N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:24468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16269"/>
            <dbReference type="ChEBI" id="CHEBI:17735"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:24469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>NADPH + O2 + H(+) = H2O2 + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:11260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.6.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:11261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>heptan-2-one + NADPH + O2 + H(+) = pentyl acetate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54836"/>
            <dbReference type="ChEBI" id="CHEBI:5672"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87362"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>heptan-4-one + NADPH + O2 + H(+) = propyl butanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:89484"/>
            <dbReference type="ChEBI" id="CHEBI:89719"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>hexan-3-one + NADPH + O2 + H(+) = ethyl butanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54844"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:88764"/>
            <dbReference type="ChEBI" id="CHEBI:89891"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hexan-3-one + NADPH + O2 + H(+) = propyl propanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54848"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:89828"/>
            <dbReference type="ChEBI" id="CHEBI:89891"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:54849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>octan-3-one + NADPH + O2 + H(+) = ethyl hexanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:80946"/>
            <dbReference type="ChEBI" id="CHEBI:86055"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:54857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>octan-3-one + NADPH + O2 + H(+) = pentyl propanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:80946"/>
            <dbReference type="ChEBI" id="CHEBI:87373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>sulcatone + NADPH + O2 + H(+) = 4-methylpent-3-en-1-yl acetate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16310"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:54865"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 29 30">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 29">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 29 30">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="30"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ETQ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035945.1">
        <property type="protein sequence ID" value="ENSBIXP00000040379.1"/>
        <property type="gene ID" value="ENSBIXG00000024454.1"/>
    </dbReference>
    <dbReference type="OMA" id="CCTGYDI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000042">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000073">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000409">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002257">
        <property type="entry name" value="Flavin_mOase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01125">
        <property type="entry name" value="FMOXYGENASE5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 29" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 29 30" id="KW-0274">FAD</keyword>
    <keyword evidence="7 29 30" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="15 29 30" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12 29" id="KW-0521">NADP</keyword>
    <keyword evidence="14 29 30" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRNR" id="PIRNR000332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="533" mass="60311" checksum="EFE2419A36BA0DE0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EV25</accession>
    <name>A0A4W2EV25_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CLDN11</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with tetraspanin-3/TSPAN3. Interacts with OCLN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell junction</location>
            <location evidence="11">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397169.1">
        <property type="nucleotide sequence ID" value="XM_027541368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EV25"/>
    <dbReference type="SMR" id="A0A4W2EV25"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050108.1">
        <property type="protein sequence ID" value="ENSBIXP00000040627.1"/>
        <property type="gene ID" value="ENSBIXG00000024743.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019053.1">
        <property type="protein sequence ID" value="ENSBIXP00005034257.1"/>
        <property type="gene ID" value="ENSBIXG00005015126.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892492"/>
    <dbReference type="CTD" id="5010"/>
    <dbReference type="GeneTree" id="ENSGT00890000139496"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000015">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003555">
        <property type="entry name" value="Claudin11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01384">
        <property type="entry name" value="CLAUDIN11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="208" mass="22157" checksum="1A1C8C4A93A5964E" modified="2019-09-18" version="1">MMVATCLQVVGFVTSFVGWIGIIVTTSTNDWVVTCGYTIPTCRKLDELGSKGLWADCVMATGLYHCKPLVDILILPGYVQACRALMIAASVLGLPAILLLLTVLPCIRMGHEPGVAKYRRAQLAGVMLILVALCAMVATIWFPVCAHRETTIVSFGYSLYAGWIGAVLCLVGGCVIVCCAGDAQAFGENRFYYSSGSSSPTHAKSAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVP4</accession>
    <name>A0A4W2EVP4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat and SOCS box protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ASB1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EVP4"/>
    <dbReference type="STRING" id="30522.A0A4W2EVP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037008.1">
        <property type="protein sequence ID" value="ENSBIXP00000040852.1"/>
        <property type="gene ID" value="ENSBIXG00000005106.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03720">
        <property type="entry name" value="SOCS_ASB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000119">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051637">
        <property type="entry name" value="Ank_repeat_dom-contain_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037331">
        <property type="entry name" value="ASB1_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 2C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180:SF45">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE TANKYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
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            <dbReference type="PROSITE" id="PS50225"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040852.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="358" mass="38913" checksum="858EB39414F09EDB" modified="2019-09-18" version="1">VPLSVRAEPGRCPSAVVLLVPVLSSCASGFLVCGPGLLRQAWAWQGECVKASPAAEPLEAFLTRGLPPAPGRRVLRLPAWLPVLSLSRINEKSVWCCGWLPCTPLRIAATAGHGNCVDFLIRKGAEVDLVDVKGQTALYVAVVNGHLESAQILLEAGADPNGSRHHRSTPVYHASRVGRADILKALIRYGADVDVNHHLTPDAQPPFSRRLTSLVVCPLYISAAYHNLQCFKLLLQAGANPDFNCNGPVNTQGFYRGSPGCVMDAVLRHGCEAAFVSLLVEFGANLNLVKWESLGPESRGRRKVDPEALQVFKEARSVPRSLLNLCRVAVRRALGKYRLHLIPSLPLPDPIKKFLLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYJ2</accession>
    <name>A0A4W2EYJ2_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="gene ID" value="ENSBIXG00000005467.1"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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        <location>
            <begin position="117"/>
            <end position="385"/>
        </location>
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        <location>
            <begin position="298"/>
            <end position="344"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="574" mass="66277" checksum="D4C2FFC0FC12C72A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZ41</accession>
    <name>A0A4W2EZ41_BOBOX</name>
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        <recommendedName>
            <fullName evidence="3">G protein-activated inward rectifier potassium channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inward rectifier K(+) channel Kir3.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Potassium channel, inwardly rectifying subfamily J member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">KCNJ3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027560348.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00000042035.1"/>
        <property type="gene ID" value="ENSBIXG00000005427.1"/>
    </dbReference>
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        <property type="gene ID" value="ENSBIXG00005019661.1"/>
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    <dbReference type="GeneTree" id="ENSGT01080000257365"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990566">
        <property type="term" value="C:I(KACh) inward rectifier potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098688">
        <property type="term" value="C:parallel fiber to Purkinje cell synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015467">
        <property type="term" value="F:G-protein activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_inward-rec_Kir3.1"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF16">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01327">
        <property type="entry name" value="KIR31CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 16" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <keyword evidence="7 16" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="19">
        <location>
            <begin position="47"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="20">
        <location>
            <begin position="194"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="501" mass="56547" checksum="E388B6FF8187CABE" modified="2019-09-18" version="1">MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQGPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQSEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDISTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGATRMEGNLPAKLRKMNSDRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZW8</accession>
    <name>A0A4W2EZW8_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZW8"/>
    <dbReference type="STRING" id="30522.A0A4W2EZW8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040634.1">
        <property type="protein sequence ID" value="ENSBIXP00000042635.1"/>
        <property type="gene ID" value="ENSBIXG00000026595.1"/>
    </dbReference>
    <dbReference type="OMA" id="FYAMGSV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017081">
        <property type="term" value="F:chloride channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006821">
        <property type="term" value="P:chloride transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029181">
        <property type="entry name" value="Barttin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28399">
        <property type="entry name" value="BARTTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28399:SF1">
        <property type="entry name" value="BARTTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15462">
        <property type="entry name" value="Barttin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="319" mass="34640" checksum="C0C6921274B612E9" modified="2019-09-18" version="1">MTDEKTFRIGFIVLGLFLLALGTFLMSHDRPQVYGTFYAMGGVMVIGGVIWSMCQCYPKITFIPADSDFQGVLSTKAKALGLLESGFAAEMKSPQPPYVRLWEAAAYDQSLPDFSHIQMKVMSYSEDPRPLLDPEPGQRQPGASDGGESGPHDPQTWLEATVVIHRGSDQDEGEGDPSQGRLSPPTCPQGSAPLASFQDDLDVGSSEGSSPNPSPPEEAEPQPPPPESRTCRCQLDRFHDFALIDAPATEDAPPEKQRWEAAVPSSQQRSPRTKEEEEASDTGTEGPAQEEEDLYYGLPDSPGDPLPDKELGFEPEAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F071</accession>
    <name>A0A4W2F071_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC113890409</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cell membrane</location>
            <topology evidence="2 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F071"/>
    <dbReference type="SMR" id="A0A4W2F071"/>
    <dbReference type="STRING" id="30522.A0A4W2F071"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040851.1">
        <property type="protein sequence ID" value="ENSBIXP00000042754.1"/>
        <property type="gene ID" value="ENSBIXG00000005649.1"/>
    </dbReference>
    <dbReference type="OMA" id="KLFCPQK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15914">
        <property type="entry name" value="7tmA_OR6N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF402">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0552">Olfaction</keyword>
    <keyword evidence="12 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="14 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="42"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="316" mass="35763" checksum="5B326817C529CD48" modified="2019-09-18" version="1">MVRSNQTSMVTEFFFSGLPELEDGNLLFFIPLLVIYIFIIIGNLIVFFAVRIDTHLHNPMYNFISIFSFLEIWYTTATIPKMLSNLVSKKRTISITGCLLQMYFFHSLGNSEGILLTTMAIDRYVAICNPLRYPTIMTPRLCTQLSIGSCIFGFLLLLPEIVWISTLPFCGPNQIHQIFCDFEPVLCLACTDTSMILIEDVIHAIAIIFSVLIIALSYIRIITVILRIPSAEGRQKAFSTCASHLGVFLMFYGSVSLMYLRFSATFPPILDTIIALMFAVLAPFFNPIIYSLRNKDMKIAIKKLLCLQKVFYASAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2V0</accession>
    <name>A0A4W2F2V0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Cytochrome P450</fullName>
            <ecNumber evidence="26">1.14.14.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">HADH</name>
        <name evidence="28" type="synonym">CYP2U1</name>
    </gene>
    <organism evidence="27 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">A cytochrome P450 monooxygenase involved in the metabolism of arachidonic acid and its conjugates. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Acts as an omega and omega-1 hydroxylase for arachidonic acid and possibly for other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. May down-regulate the biological activities of N-arachidonoyl-serotonin, an endocannabinoid that has anti-nociceptive effects through inhibition of fatty acid amide hydrolase FAAH, TRPV1 receptor and T-type calcium channels. Catalyzes C-2 oxidation of the indole ring of N-arachidonoyl-serotonin forming a less active product 2-oxo-N-arachidonoyl-serotonin.</text>
    </comment>
    <comment type="function">
        <text evidence="26">Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39759"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:76627"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:39760"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + reduced [NADPH--hemoprotein reductase] + O2 = 20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39755"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:76624"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:39756"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N-[(5Z,8Z,11Z,14Z)-eicosatetraenoyl]-serotonin + reduced [NADPH--hemoprotein reductase] + O2 = 2-oxo-N-[(5Z,8Z,11Z,14Z)-eicosatetraenoyl]-serotonin + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:132255"/>
            <dbReference type="ChEBI" id="CHEBI:132256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:50297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an omega-methyl-long-chain fatty acid + reduced [NADPH--hemoprotein reductase] + O2 = an omega-hydroxy-long-chain fatty acid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56748"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:140991"/>
            <dbReference type="ChEBI" id="CHEBI:140992"/>
            <dbReference type="EC" id="1.14.14.80"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:56749"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24 26">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 26">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 26">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 25">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.-" evidence="26"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F2V0"/>
    <dbReference type="SMR" id="A0A4W2F2V0"/>
    <dbReference type="STRING" id="30522.A0A4W2F2V0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042037.1">
        <property type="protein sequence ID" value="ENSBIXP00000043373.1"/>
        <property type="gene ID" value="ENSBIXG00000027476.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035727.1">
        <property type="protein sequence ID" value="ENSBIXP00005021730.1"/>
        <property type="gene ID" value="ENSBIXG00005024695.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157714"/>
    <dbReference type="OMA" id="EPCIQQG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052869">
        <property type="term" value="F:arachidonate omega-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102033">
        <property type="term" value="F:long-chain fatty acid omega-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097267">
        <property type="term" value="P:omega-hydroxylase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20666">
        <property type="entry name" value="CYP2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000017">
        <property type="entry name" value="cytochrome P450 2U1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008069">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF364">
        <property type="entry name" value="CYTOCHROME P450 2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01686">
        <property type="entry name" value="EP450ICYP2D"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 24 25" id="KW-0349">Heme</keyword>
    <keyword evidence="14 24 25" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 24 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15 25" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="13 25" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="24">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00058812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU368047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="543" mass="62031" checksum="6E908B438BF8BF1A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F2W6</accession>
    <name>A0A4W2F2W6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Growth arrest and DNA damage-inducible protein GADD45 alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GADD45A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GADD45 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027392744.1">
        <property type="nucleotide sequence ID" value="XM_027536943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F2W6"/>
    <dbReference type="SMR" id="A0A4W2F2W6"/>
    <dbReference type="STRING" id="30522.A0A4W2F2W6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041986.1">
        <property type="protein sequence ID" value="ENSBIXP00000043367.1"/>
        <property type="gene ID" value="ENSBIXG00000027447.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033621.1">
        <property type="protein sequence ID" value="ENSBIXP00005020305.1"/>
        <property type="gene ID" value="ENSBIXG00005023475.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889749"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182964"/>
    <dbReference type="OMA" id="FRMTFEE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043537">
        <property type="term" value="P:negative regulation of blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900745">
        <property type="term" value="P:positive regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000379">
        <property type="term" value="P:positive regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042770">
        <property type="term" value="P:signal transduction in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000012">
        <property type="entry name" value="growth arrest and DNA damage-inducible protein GADD45 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF3">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="9">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="165" mass="18337" checksum="FFCDCCE3F6C85AC6" modified="2019-09-18" version="1">MTLEEFSAGEQKTERMDKVGDALEEVLSKALSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIQAFCCENDIDILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3M8</accession>
    <name>A0A4W2F3M8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1</fullName>
            <ecNumber evidence="5">2.4.99.20</ecNumber>
            <ecNumber evidence="4">3.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">2'-phospho-ADP-ribosyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">2'-phospho-cyclic-ADP-ribose transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">ADP-ribosyl cyclase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cyclic ADP-ribose hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.20" evidence="5"/>
    <dbReference type="EC" id="3.2.2.6" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3M8"/>
    <dbReference type="STRING" id="30522.A0A4W2F3M8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042509.1">
        <property type="protein sequence ID" value="ENSBIXP00000043658.1"/>
        <property type="gene ID" value="ENSBIXG00000007881.1"/>
    </dbReference>
    <dbReference type="OMA" id="GMYVTGC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04759">
        <property type="entry name" value="Rib_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF5">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="348" mass="38938" checksum="D7124E48673FE84C" modified="2019-09-18" version="1">MRSNQPSLALHGIPVIYCCLFSKPHPKLSAIKQHPFYQRSPLRDFEGGGRSRKGSERKGEPALKGKCSLRNGLALSSPAAQTPAAASTNWSGRPSSDPMADCCTPIKKLCWWHGVGSTADFQKIIQERCDTYTQTIRPGSRSRNCQAIRQAFMSTFISKDPCKATKEDYNSLINLAPPTVPCGQQVFWSKTKELAHEYVKRQRLMTLEDTLLGYLADGLSWCGEPGSSDLNIWSCPDWRKDCRTNYLSVFWEVLSERFAESACSTVRVVLNGSLENAFDSMSIFGRVEAPNLRPQVELEAWLVHDTGKPPSDSCSGSSIRKLKSILDGRNVKFRCMDNLSRDQFLQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F892</accession>
    <name>A0A4W2F892_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDB1- and CUL4-associated factor 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">WD repeat-containing protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DCAF10</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DDB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat DCAF10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405494.1">
        <property type="nucleotide sequence ID" value="XM_027549693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F892"/>
    <dbReference type="SMR" id="A0A4W2F892"/>
    <dbReference type="STRING" id="30522.A0A4W2F892"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046654.1">
        <property type="protein sequence ID" value="ENSBIXP00000014712.1"/>
        <property type="gene ID" value="ENSBIXG00000003432.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014078.1">
        <property type="protein sequence ID" value="ENSBIXP00005001540.1"/>
        <property type="gene ID" value="ENSBIXG00005000071.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897210"/>
    <dbReference type="CTD" id="79269"/>
    <dbReference type="GeneTree" id="ENSGT00390000012666"/>
    <dbReference type="OMA" id="STAHEDC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="DDB1- and CUL4-associated factor 10"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DCA10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14588">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14588:SF2">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5 10" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="211"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="354"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="564" mass="61512" checksum="588FF78C45CC0C10" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FC91</accession>
    <name>A0A4W2FC91_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Filensin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Beaded filament structural protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">BFSP1</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA. Involved in altering the calcium regulation of MIP water permeability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Part of a complex required for lens intermediate filament formation composed of BFSP1, BFSP2 and CRYAA. Identified in a complex that contains VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin. Found in a complex composed of PPL (via C-terminal linker domain), BFSP1 and BFSP2 in the retinal lens. Within the complex interacts with BFSP2. Interacts (via C-terminus) with MIP (via C-terminus) in aged lens fiber cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027415477.1">
        <property type="nucleotide sequence ID" value="XM_027559676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FC91"/>
    <dbReference type="STRING" id="30522.A0A4W2FC91"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024146.1">
        <property type="protein sequence ID" value="ENSBIXP00000035295.1"/>
        <property type="gene ID" value="ENSBIXG00000018539.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011192.1">
        <property type="protein sequence ID" value="ENSBIXP00005002963.1"/>
        <property type="gene ID" value="ENSBIXG00005009213.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903494"/>
    <dbReference type="CTD" id="631"/>
    <dbReference type="GeneTree" id="ENSGT00390000016976"/>
    <dbReference type="OMA" id="IQTTPRV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048469">
        <property type="term" value="P:cell maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070307">
        <property type="term" value="P:lens fiber cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000094">
        <property type="entry name" value="Beaded filament structural protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000009">
        <property type="entry name" value="filensin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042358">
        <property type="entry name" value="BFSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14069">
        <property type="entry name" value="FILENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14069:SF0">
        <property type="entry name" value="FILENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="11" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="IF rod" evidence="22">
        <location>
            <begin position="39"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="493"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="43"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="234"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="528"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005002963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="757" mass="83182" checksum="164237313CE0B977" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FCA9</accession>
    <name>A0A4W2FCA9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TEX261</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TEX261</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410742.1">
        <property type="nucleotide sequence ID" value="XM_027554941.1"/>
    </dbReference>
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        <source>
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        <source>
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        </source>
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    <sequence length="196" mass="22600" checksum="92EEE0DE5232E844" modified="2019-09-18" version="1">MWFMYVLSWLSLFIQVAFITLAVAAGLYYLAELIEEYTVATSRIIKYMIWFSTAVLIGLYVFERFPTYMIGVGLFTNLVYFGLLQTFPFIMLTSPNFILSCGLVVVNHYLAFQFFAEEYYPFSEVLAYFTFCLWIIPFAFFVSLSAGENVLPSTMQPGDDVVSNYFTKGKRGKRLGILVVFSFIKEAILPSRQKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FJB6</accession>
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        <submittedName>
            <fullName evidence="6">Small integral membrane protein 18</fullName>
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        <name evidence="6" type="primary">SMIM18</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="SIM_Modulators"/>
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        <property type="entry name" value="CLCA DOMAIN-CONTAINING PROTEIN"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="98" mass="11378" checksum="8257CDB588EB0DBA" modified="2019-09-18" version="1">MASLSSSLWNETTTSVYQYLGFQVQKIYPFHDNWNTACFVILLLFIFTVVSLVVLAFLYEVLDCCCCVKNKTVKDLKNEPNPIRSMMDNIRKRETEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FQP6</accession>
    <name>A0A4W2FQP6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="13">GDNF family receptor alpha-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GFRA3</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for artemin (ARTN), a growth factor that supports the survival of sensory and sympathetic peripheral neurons. ARTN-binding leads to autophosphorylation and activation of the RET receptor.</text>
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    <comment type="subunit">
        <text evidence="12">Interacts with ARTN ligand and RET: forms a 2:2:2 ternary complex composed of ARTN ligand, GFRA3 and RET receptor. Interacts with SORL1.</text>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
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        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the GDNFR family.</text>
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        <property type="nucleotide sequence ID" value="XM_027546380.1"/>
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        <property type="gene ID" value="ENSBIXG00000005375.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005006909.1"/>
        <property type="gene ID" value="ENSBIXG00005001287.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000161256"/>
    <dbReference type="OMA" id="CNAAYQH"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008046">
        <property type="term" value="F:axon guidance receptor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016167">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048485">
        <property type="term" value="P:sympathetic nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="GDNF family receptor alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.110">
        <property type="entry name" value="GDNF binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003505">
        <property type="entry name" value="GDNF_rcpt_A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF15">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01319">
        <property type="entry name" value="GDNFRALPHA3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01316">
        <property type="entry name" value="GDNFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611431" description="GDNF family receptor alpha-3" evidence="14">
        <location>
            <begin position="25"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="15">
        <location>
            <begin position="38"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="15">
        <location>
            <begin position="156"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="15">
        <location>
            <begin position="242"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="394" mass="44068" checksum="88B7D038C61C8791" modified="2019-09-18" version="1">MARPQNPRPLPPALLLLLPLLLRAGDHLPTEGRFTNSCIQARRKCQADPTCNATYHYLNSCASSISTSSPAEEPLVPEDCMEAAQQLRNSSLMSCTCHRRMKNQATCLDIYWTIHPARSLGDYELDVSPYEDTVTRKPWKMNLSKLNMLIPDSDLCLKFAMLCTLNDKCDRLRKAYGEACSGSRCQRHTCQRQLRAFFEKASEPHSQGLLLCPCAPTDQGCGQRRRNTIAPSCALPSEAPNCLELRHVCVSDPLCRSRLADFQTHCHPMDILGTCATEQSRCLRAYMGLIGTALTPNFVSNINASVALSCTCRGSGNLQEECERLEESFSHNPCLMEAISAKMRFHSQLFSQDWAASTFSVMERQNKNPALRPQPWVPTLFSCTLTLILLSSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GC80</accession>
    <name>A0A4W2GC80_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Double-stranded RNA-binding protein Staufen homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">STAU1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027414113.1">
        <property type="nucleotide sequence ID" value="XM_027558312.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414114.1">
        <property type="nucleotide sequence ID" value="XM_027558313.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414116.1">
        <property type="nucleotide sequence ID" value="XM_027558315.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414119.1">
        <property type="nucleotide sequence ID" value="XM_027558318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GC80"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025909.1">
        <property type="protein sequence ID" value="ENSBIXP00000014814.1"/>
        <property type="gene ID" value="ENSBIXG00000019425.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026403.1">
        <property type="protein sequence ID" value="ENSBIXP00005015504.1"/>
        <property type="gene ID" value="ENSBIXG00005019320.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902924"/>
    <dbReference type="CTD" id="6780"/>
    <dbReference type="GeneTree" id="ENSGT00940000157304"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032839">
        <property type="term" value="C:dendrite cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098964">
        <property type="term" value="P:anterograde dendritic transport of messenger ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035418">
        <property type="term" value="P:protein localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19881">
        <property type="entry name" value="DSRM_STAU1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19885">
        <property type="entry name" value="DSRM_STAU1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19887">
        <property type="entry name" value="DSRM_STAU1_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000007">
        <property type="entry name" value="Double-stranded RNA-binding protein Staufen homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000014">
        <property type="entry name" value="double-stranded RNA-binding protein Staufen homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000024">
        <property type="entry name" value="double-stranded RNA-binding protein Staufen homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000013">
        <property type="entry name" value="double-stranded RNA-binding protein Staufen homolog 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051740">
        <property type="entry name" value="DRBM-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032478">
        <property type="entry name" value="Staufen_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46054:SF2">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING PROTEIN STAUFEN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46054">
        <property type="entry name" value="MATERNAL EFFECT PROTEIN STAUFEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16482">
        <property type="entry name" value="Staufen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="103"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="211"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005015504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="502" mass="55373" checksum="291D3202CAE223B6" modified="2019-09-18" version="1">MKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQSEPPSERLEVNGRESEEENLNKSEISQVFEIALKRNLPVNFESFPPTQVARESGPPHMKSFVTRVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPTVERVKPRIKKKTKSIVRLQGGPECGQGMNPISRLAQIQQAKKEKEPEYLLLAERGLPRRREFVMQVKVGNHTAEGSGTNKKVAKRNAAENMLEILGFKVPQAQPSKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFARVAPNPAKATVTAMIARELLYGGTSPTAETILKSNISSGHAPHGPLTRPSEQLDYLCTVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQNTEMPRTGNGPMSVCGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GJX0</accession>
    <name>A0A4W2GJX0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin-D-binding Myb-like transcription factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DMTF1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with the D-type cyclins CCND1, CCND2 and CCND3. Interaction with D-type cyclins may modulate transcriptional activation by this protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DMTF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027395649.1">
        <property type="nucleotide sequence ID" value="XM_027539848.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395650.1">
        <property type="nucleotide sequence ID" value="XM_027539849.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395651.1">
        <property type="nucleotide sequence ID" value="XM_027539850.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395652.1">
        <property type="nucleotide sequence ID" value="XM_027539851.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027395653.1">
        <property type="nucleotide sequence ID" value="XM_027539852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GJX0"/>
    <dbReference type="SMR" id="A0A4W2GJX0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041646.1">
        <property type="protein sequence ID" value="ENSBIXP00000043174.1"/>
        <property type="gene ID" value="ENSBIXG00000027246.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030659.1">
        <property type="protein sequence ID" value="ENSBIXP00005018331.1"/>
        <property type="gene ID" value="ENSBIXG00005021807.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113891600"/>
    <dbReference type="CTD" id="9988"/>
    <dbReference type="GeneTree" id="ENSGT00940000156016"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000114">
        <property type="entry name" value="cyclin-D-binding Myb-like transcription factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000139">
        <property type="entry name" value="cyclin-D-binding Myb-like transcription factor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046775">
        <property type="entry name" value="DMTF1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
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    </dbReference>
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        <property type="entry name" value="DMTF1_N"/>
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        <property type="entry name" value="Myb_DNA-binding"/>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="HTH_MYB"/>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="14">
        <location>
            <begin position="268"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="13">
        <location>
            <begin position="268"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="737"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="749"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        <source>
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    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018331.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="761" mass="84615" checksum="8DF5DF1405A73EB7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GNE8</accession>
    <name>A0A4W2GNE8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 21">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16 21">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">JAK1</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Kinase partner for the type I interferon receptor IFNAR2. In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins. Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16 21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000027242.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005031044.1">
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        <property type="gene ID" value="ENSBIXG00005021985.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157092"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:extrinsic component of cytoplasmic side of plasma membrane"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0060397">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006357">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
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    <dbReference type="GO" id="GO:0060337">
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="Tyr_kinase_non-rcpt_Jak/Tyk2"/>
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        <property type="entry name" value="Tyr_kinase_non-rcpt_Jak1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="JANUSKINASE"/>
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    <dbReference type="PRINTS" id="PR01824">
        <property type="entry name" value="JANUSKINASE1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
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        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 19" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3 16 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 16 21" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FERM" evidence="24">
        <location>
            <begin position="34"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="22">
        <location>
            <begin position="439"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="515"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="826"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="954"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="832"/>
            <end position="840"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="859"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000636-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000636-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1110" mass="128234" checksum="FEE10F78D654F148" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GPT6</accession>
    <name>A0A4W2GPT6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="12">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 15" type="primary">SNRPD2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10 12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
        <text evidence="12">SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371247.1">
        <property type="nucleotide sequence ID" value="XM_027515446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GPT6"/>
    <dbReference type="SMR" id="A0A4W2GPT6"/>
    <dbReference type="STRING" id="30522.A0A4W2GPT6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022266.1">
        <property type="protein sequence ID" value="ENSBIXP00000034477.1"/>
        <property type="gene ID" value="ENSBIXG00000017480.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050050.1">
        <property type="protein sequence ID" value="ENSBIXP00005019928.1"/>
        <property type="gene ID" value="ENSBIXG00005006167.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876333"/>
    <dbReference type="CTD" id="6633"/>
    <dbReference type="GeneTree" id="ENSGT00390000017608"/>
    <dbReference type="OMA" id="DVKEMWT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034709">
        <property type="term" value="C:methylosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000069">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="29"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="118" mass="13527" checksum="D986059D82B7E045" modified="2019-09-18" version="1">MSLLNKPKSEMTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFDRHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H0H6</accession>
    <name>A0A4W2H0H6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA splicing endonuclease subunit 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TSEN15</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H0H6"/>
    <dbReference type="STRING" id="30522.A0A4W2H0H6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016029.1">
        <property type="protein sequence ID" value="ENSBIXP00000008375.1"/>
        <property type="gene ID" value="ENSBIXG00000008039.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039742.1">
        <property type="protein sequence ID" value="ENSBIXP00005024498.1"/>
        <property type="gene ID" value="ENSBIXG00005027043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014781"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28582">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28582:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="4">
        <location>
            <begin position="305"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="411" mass="43864" checksum="F24DA3387883C8F4" modified="2019-09-18" version="1">MRGEEKRNLRECKGGEASTFAASPQPYLGEARGHPIRTVPGGAPCGLLIPRSTRGAHRRARPSRPQPLIRPGPGQAARGVRTMSPARPELPPDPPARRGGLTRWPSCSWGSPGAPGSRGSEAACAQRCARGRPRAPWVSAGATRGKPPPPLRRPRSPPRRRSAPGSRRAAEPRRGAAAPRPGGRRGARPCSGPWRAAGRPGADARGRRRGAEGRRLARAHWHGLGRRDPPAAAAAAPPRPRRGRSGVPAAAGARLGSGSQTVVSVRLFVPSPAESSSSSGSACTEYLEMMELDIGDATQVYIAFLVYLDLMESKSWHEVNCVGLPDLQLICLLGTEIEGEGLQTVVPTPISASLSHNRIREILKASRKLQGDPDLPTSFTLAIVESDSTIVYYKLTDGFMLPDPQNISLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H242</accession>
    <name>A0A4W2H242_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-Fucosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC113876245</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ganglioside GM1 + GDP-beta-L-fucose = a ganglioside Fuc-GM1 + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:82639"/>
            <dbReference type="ChEBI" id="CHEBI:90189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:48293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">Golgi stack membrane</location>
            <topology evidence="1 8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyltransferase 11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_027371032.1">
        <property type="nucleotide sequence ID" value="XM_027515231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H242"/>
    <dbReference type="STRING" id="30522.A0A4W2H242"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045838.1">
        <property type="protein sequence ID" value="ENSBIXP00000012973.1"/>
        <property type="gene ID" value="ENSBIXG00000017974.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038906.1">
        <property type="protein sequence ID" value="ENSBIXP00005023862.1"/>
        <property type="gene ID" value="ENSBIXG00005026530.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876245"/>
    <dbReference type="GeneTree" id="ENSGT00390000001450"/>
    <dbReference type="OMA" id="GLQAKWA"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008107">
        <property type="term" value="F:galactoside 2-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11301">
        <property type="entry name" value="Fut1_Fut2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002516">
        <property type="entry name" value="Glyco_trans_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927:SF8">
        <property type="entry name" value="GALACTOSIDE 2-ALPHA-L-FUCOSYLTRANSFERASE SEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927">
        <property type="entry name" value="GALACTOSIDE 2-L-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01531">
        <property type="entry name" value="Glyco_transf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="454" mass="50343" checksum="EBF14D9810C1A9BF" modified="2019-09-18" version="1">MGVASAKGGERGAGQGRARGQGICRSRKSRPGKARARKDLVLECFSSPSRAGSPRWRAPSGLGALNPSGPHSPGPWGPLAAIPPGTMWDMRAVAPQRPAAGHPRAGWPRKLKTAATRFWATCPSSSTVCFLFVIFAVSTVFHCHRRLALVPAPWAYAGHVVLFPRHLPRGGVFTINAKGRLGNQMGEYATLYALAKMNGRAAFIPPQMHSTLAPIFRITLPVLHDATARSVPWQNYHLNDWMEEQYRHIPGEYVRLTGYPCSWTFYHHLRAEILQEFTLHAHVREEAQNFLRGLRVNGSRPSTYVGVHVRRGDYVRVMPTMWKGVLADRGYLQQALDWFRARHHSPLFVITSDDMAWCRRNINSSHRDVVFAGSGQQGSPARDFALLTQCNHTVITVGTFGIWAAYLAGGSTVYLANFTLPGSRFRMIFKPQAAFLPEWVGIAANLGQARESHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H4S9</accession>
    <name>A0A4W2H4S9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor 2M3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC113896597</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027404628.1">
        <property type="nucleotide sequence ID" value="XM_027548827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H4S9"/>
    <dbReference type="STRING" id="30522.A0A4W2H4S9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036585.1">
        <property type="protein sequence ID" value="ENSBIXP00000021739.1"/>
        <property type="gene ID" value="ENSBIXG00000006684.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040039.1">
        <property type="protein sequence ID" value="ENSBIXP00005024693.1"/>
        <property type="gene ID" value="ENSBIXG00005027211.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896597"/>
    <dbReference type="GeneTree" id="ENSGT01150000286923"/>
    <dbReference type="OMA" id="FISMAGC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15421">
        <property type="entry name" value="7tmA_OR2T-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000583">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="311" mass="34885" checksum="C8AE7AD3F2627568" modified="2019-09-18" version="1">MAWEFQTFNSDFILLGIFDHRPTHIFLFSRVLGVSAVAIMGNTLVVLFLYLNAQLHTPMYFLLSQLSLTDLVLISTTIPKMAFNYLSGSKSITMADCATHIFFYASLTGCECFLLAVMAYDCDVATCHPLRYTSLMRLKICGLMTASSWVLGSTDGVIDAVVTFSFSCCGFWVIAHFFCDFPFLLVIQCNDTSIFEEVLFYCCIMMILFPVAIIIASYARVLLTVILMGSGKGRHRAFATCSSHLMVVGTYYGAVLFMYMRPASNHSPTQDRMMSVFYTILTPTLNPLIYSFRNKEVACAFQVFRKGKTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H5Q1</accession>
    <name>A0A4W2H5Q1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium inwardly rectifying channel subfamily J member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KCNJ1</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H5Q1"/>
    <dbReference type="SMR" id="A0A4W2H5Q1"/>
    <dbReference type="STRING" id="30522.A0A4W2H5Q1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013909.1">
        <property type="protein sequence ID" value="ENSBIXP00000001490.1"/>
        <property type="gene ID" value="ENSBIXG00000028503.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042102.1">
        <property type="protein sequence ID" value="ENSBIXP00005026076.1"/>
        <property type="gene ID" value="ENSBIXG00005028469.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282491"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000036">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000002">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF6">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 15" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="17">
        <location>
            <begin position="37"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="18">
        <location>
            <begin position="186"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="14">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="385" mass="44174" checksum="B5617653A3A00E27" modified="2019-09-18" version="1">MLAIHEISVLTERMFKHLRKWFVTRFFGRSRRRARLVSKDGRCNIEFGNVEAQSRLIFFVDIWTTVLDLKWRYKMTIFITAFLGSWFFFGLLWYVVAYVHKDLPEFHPPANHTPCVEHINGLTSAFLFSLETQVTIGYGFRCVTEQCGTAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLRTTITPEGETIILDQININFVVDAGNENLFFISPLTIYHVIDHTSPFFHMAAETLPQQDFELVVFLDGTVEATSATCQVRTSYVPEEVLWGYRFAPIVSKTKEGKYRVDFHNFSKTVEVETPHCAMCLYNEKDARAKMKRGYDNPNFVLSEVNETDDTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HUR1</accession>
    <name>A0A4W2HUR1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitotic spindle assembly checkpoint protein MAD2A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mitotic arrest deficient 2-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HUR1"/>
    <dbReference type="STRING" id="30522.A0A4W2HUR1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053621.1">
        <property type="protein sequence ID" value="ENSBIXP00000000191.1"/>
        <property type="gene ID" value="ENSBIXG00000030132.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017559.1">
        <property type="protein sequence ID" value="ENSBIXP00005033612.1"/>
        <property type="gene ID" value="ENSBIXG00005014429.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153395"/>
    <dbReference type="OMA" id="ICAFDLK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990728">
        <property type="term" value="C:mitotic spindle assembly checkpoint MAD1-MAD2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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            <dbReference type="ARBA" id="ARBA00076594"/>
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        <source>
            <dbReference type="PROSITE" id="PS50815"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033612.1"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="197" mass="22297" checksum="5CD6687BFC814531" modified="2019-09-18" version="1">MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGLYPSETFTRVQKYGLTLLVTTDPELIKYLNNVDQLKGILLLDTCSVQKLVVVISNIESGEVLERWQFDIDAPREKFQKAIQDEICSVVRQITATVTFLPLLEVSCSFDLLICTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSVVAYTIPVND</sequence>
</entry>
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    <accession>A0A4W2HZJ6</accession>
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        <name evidence="11" type="primary">GPR75</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Laurasiatheria</taxon>
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    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
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                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="540" mass="59483" checksum="582554881C2AEC5B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I6Q4</accession>
    <name>A0A4W2I6Q4_BOBOX</name>
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        <recommendedName>
            <fullName evidence="5">Selenoprotein H</fullName>
        </recommendedName>
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        <name evidence="7" type="primary">SELENOH</name>
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    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="3">May be involved in a redox-related process.</text>
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        <text evidence="4">Belongs to the SelWTH family.</text>
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    <dbReference type="OMA" id="WHNAEEV"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Selenoprotein H"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005041023.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="112" mass="12084" checksum="8F6C31D3CF536428" modified="2019-09-18" version="1">MASRGRKRKAEAALAAAAEKREKPAGGQEGGVEGPIYGRNAAALSQALRLQAPELTVKVNPARPRRGSFEVTLLRADGSSAELWTGLKKGPPRKLKFPEPHVVLEELKKYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I9B7</accession>
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        <recommendedName>
            <fullName evidence="17">Solute carrier family 41 member</fullName>
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        <name evidence="19" type="primary">SLC41A2</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
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    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Acts as a plasma-membrane magnesium transporter. Can also mediate the transport of other divalent metal cations in an order of Ba(2+) &gt; Ni(2+) &gt; Co(2+) &gt; Fe(2+) &gt; Mn(2+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Co(2+)(in) = Co(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28578"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Mn(2+)(in) = Mn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28699"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ni(2+)(in) = Ni(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29831"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397712.1">
        <property type="nucleotide sequence ID" value="XM_027541911.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397713.1">
        <property type="nucleotide sequence ID" value="XM_027541912.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397714.1">
        <property type="nucleotide sequence ID" value="XM_027541913.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397715.1">
        <property type="nucleotide sequence ID" value="XM_027541914.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397717.1">
        <property type="nucleotide sequence ID" value="XM_027541916.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397718.1">
        <property type="nucleotide sequence ID" value="XM_027541917.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397719.1">
        <property type="nucleotide sequence ID" value="XM_027541918.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027397720.1">
        <property type="nucleotide sequence ID" value="XM_027541919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I9B7"/>
    <dbReference type="STRING" id="30522.A0A4W2I9B7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041874.1">
        <property type="protein sequence ID" value="ENSBIXP00000025248.1"/>
        <property type="gene ID" value="ENSBIXG00000005742.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030928.1">
        <property type="protein sequence ID" value="ENSBIXP00005039484.1"/>
        <property type="gene ID" value="ENSBIXG00005003693.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892711"/>
    <dbReference type="CTD" id="84102"/>
    <dbReference type="GeneTree" id="ENSGT00950000183042"/>
    <dbReference type="OMA" id="NDKAYHG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015675">
        <property type="term" value="P:nickel cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF25">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="539"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="18">
        <location>
            <begin position="200"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="18">
        <location>
            <begin position="414"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="573" mass="62265" checksum="A3320E808F78F415" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IA09</accession>
    <name>A0A4W2IA09_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Probable thioesterase PNKD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PNKD</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Probable thioesterase that may play a role in cellular detoxification processes; it likely acts on a yet-unknown alpha-hydroxythioester substrate. In vitro, it is able to catalyze the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid at very low rate, though this reaction is not physiologically relevant in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a thioester + H2O = a thiol + a carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:51277"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027423161.1">
        <property type="nucleotide sequence ID" value="XM_027567360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IA09"/>
    <dbReference type="SMR" id="A0A4W2IA09"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025035.1">
        <property type="protein sequence ID" value="ENSBIXP00000035671.1"/>
        <property type="gene ID" value="ENSBIXG00000018970.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033858.1">
        <property type="protein sequence ID" value="ENSBIXP00005040778.1"/>
        <property type="gene ID" value="ENSBIXG00005023605.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907748"/>
    <dbReference type="CTD" id="25953"/>
    <dbReference type="GeneTree" id="ENSGT00940000158887"/>
    <dbReference type="OMA" id="CVWPGMR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046929">
        <property type="term" value="P:negative regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000015">
        <property type="entry name" value="probable hydrolase PNKD isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF116">
        <property type="entry name" value="HYDROLASE PNKD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="15">
        <location>
            <begin position="104"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="360" mass="40723" checksum="716F3818719ADDE8" modified="2019-09-18" version="1">MAWRGWPASWLWVASCGLLLLVFVLLLSPRSCRARRTLRGLFMARSKRLLFRIGYSLYTRTRLGYLFYRQQLRRARNRYPKGHSRTQPRLFNGVKVLPIPVLSDNYSYLIIDTQARLAVAVDPSDPQAVQASIEKEGVNLVAILCTHKHWDHSGGNRDLSRRHQDCRVYGSPQDSIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYEGPSCLFSGDLLFLSGCGRTFEGTAETMLSSLDTVLGLGDDTLLWPGHEYAEENLGFAGVVEPENLARERKMQWVQRQRMERKSTCPSTLGEERSYNPFLRTHCLVLQEALGPSPGPTGDDGCSRAQLLERLRQLKDLHKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IDA6</accession>
    <name>A0A4W2IDA6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IDA6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033192.1">
        <property type="protein sequence ID" value="ENSBIXP00000019547.1"/>
        <property type="gene ID" value="ENSBIXG00000023127.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034335.1">
        <property type="protein sequence ID" value="ENSBIXP00005041000.1"/>
        <property type="gene ID" value="ENSBIXG00005023825.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271662"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005041000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="26" mass="2945" checksum="FA56A851550E91FC" modified="2019-09-18" version="1">QQTSGSKMIFGKGTQLTVQLGKWRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IER4</accession>
    <name>A0A4W2IER4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Leucine rich repeat containing 3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LRRC3B</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LRRC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027385779.1">
        <property type="nucleotide sequence ID" value="XM_027529978.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385780.1">
        <property type="nucleotide sequence ID" value="XM_027529979.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385783.1">
        <property type="nucleotide sequence ID" value="XM_027529982.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385784.1">
        <property type="nucleotide sequence ID" value="XM_027529983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IER4"/>
    <dbReference type="SMR" id="A0A4W2IER4"/>
    <dbReference type="STRING" id="30522.A0A4W2IER4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003610.1">
        <property type="protein sequence ID" value="ENSBIXP00000006499.1"/>
        <property type="gene ID" value="ENSBIXG00000012610.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040553.1">
        <property type="protein sequence ID" value="ENSBIXP00005043806.1"/>
        <property type="gene ID" value="ENSBIXG00005027556.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884892"/>
    <dbReference type="CTD" id="116135"/>
    <dbReference type="GeneTree" id="ENSGT00940000157780"/>
    <dbReference type="OMA" id="PKGCACQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000069">
        <property type="entry name" value="leucine-rich repeat-containing protein 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612095" evidence="10">
        <location>
            <begin position="28"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="11">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="259" mass="29303" checksum="B085ECF266EA740A" modified="2019-09-18" version="1">MNLVDLWLTRSLSMCLLLQSFVLMILCFHSASMCPKGCLCSSSGGLNVTCSNANLKEIPRDLPPETVLLYLDSNQITSIPNEIFKDLHQLRVLNLSKNGIEFIDEHAFKGVAETLQTLDLSDNRIQSVHKNAFNNLKARARIANNPWHCDCTLQQVLRSMVSNHETAHNVICKTSVLDEHAGRPFLNAANDADLCNLPKKTTDYAMLVTMFGWFTMVISYVVYYVRQNQEDARRHLEYLKSLPSRQKKADEPDDISTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQ59</accession>
    <name>A0A4W2BQ59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein FAM234A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein ITFG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FAM234A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the FAM234 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQ59"/>
    <dbReference type="STRING" id="30522.A0A4W2BQ59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014479.1">
        <property type="protein sequence ID" value="ENSBIXP00000001227.1"/>
        <property type="gene ID" value="ENSBIXG00000028787.1"/>
    </dbReference>
    <dbReference type="OMA" id="FMFWGLM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002632">
        <property type="entry name" value="Protein FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF7">
        <property type="entry name" value="PROTEIN FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="11">
        <location>
            <begin position="81"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="552" mass="58767" checksum="4F7A628592DE9AAB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQF4</accession>
    <name>A0A4W2BQF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gla domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQF4"/>
    <dbReference type="STRING" id="30522.A0A4W2BQF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014849.1">
        <property type="protein sequence ID" value="ENSBIXP00000001061.1"/>
        <property type="gene ID" value="ENSBIXG00000029005.1"/>
    </dbReference>
    <dbReference type="OMA" id="FIGQRCK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF37">
        <property type="entry name" value="TRANSMEMBRANE GAMMA-CARBOXYGLUTAMIC ACID PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gla" evidence="2">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="64" mass="7462" checksum="8CCC780604C0E06C" modified="2019-09-18" version="1">MNYVFLTGEKANSVLKRYPRANGLFEEIRQGNIERECKEEVCTFEEAREAFENNEKTVSILAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVF0</accession>
    <name>A0A4W2BVF0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H3-like centromeric protein A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Centromere protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CENPA</name>
        <name evidence="18" type="synonym">LOC113888742</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411678.1">
        <property type="nucleotide sequence ID" value="XM_027555877.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027411679.1">
        <property type="nucleotide sequence ID" value="XM_027555878.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027411680.1">
        <property type="nucleotide sequence ID" value="XM_027555879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVF0"/>
    <dbReference type="SMR" id="A0A4W2BVF0"/>
    <dbReference type="STRING" id="30522.A0A4W2BVF0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008291.1">
        <property type="protein sequence ID" value="ENSBIXP00000004214.1"/>
        <property type="gene ID" value="ENSBIXG00000010576.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035639.1">
        <property type="protein sequence ID" value="ENSBIXP00005021664.1"/>
        <property type="gene ID" value="ENSBIXG00005002573.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901478"/>
    <dbReference type="CTD" id="1058"/>
    <dbReference type="GeneTree" id="ENSGT01150000287000"/>
    <dbReference type="OMA" id="MRWQVYA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061638">
        <property type="term" value="C:CENP-A containing chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071459">
        <property type="term" value="P:protein localization to chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000065">
        <property type="entry name" value="Histone H3-like centromeric protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF14">
        <property type="entry name" value="CENTROMERE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="44"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="138" mass="15678" checksum="56E551F0697AC4A3" modified="2019-09-18" version="1">MGPRRQKRKPETPRRRPASPAPAAPRPTPSLGTSSRPLARRRHTVLKEIRTLQKTTHLLLRKSPFCRLAREICVQFTRGVDFNWQAQALLALQEAAEAFLVHLFEDAYLLSLHAGRVTLFPKDVQLARRIRGIQEGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BX41</accession>
    <name>A0A4W2BX41_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TAFII28-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BX41"/>
    <dbReference type="STRING" id="30522.A0A4W2BX41"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009033.1">
        <property type="protein sequence ID" value="ENSBIXP00000003858.1"/>
        <property type="gene ID" value="ENSBIXG00000010244.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000009036.1">
        <property type="protein sequence ID" value="ENSBIXP00000003856.1"/>
        <property type="gene ID" value="ENSBIXG00000010242.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000009039.1">
        <property type="protein sequence ID" value="ENSBIXP00000003846.1"/>
        <property type="gene ID" value="ENSBIXG00000010239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000009041.1">
        <property type="protein sequence ID" value="ENSBIXP00000003848.1"/>
        <property type="gene ID" value="ENSBIXG00000010238.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000020292.1">
        <property type="protein sequence ID" value="ENSBIXP00000033631.1"/>
        <property type="gene ID" value="ENSBIXG00000002248.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000044952.1">
        <property type="protein sequence ID" value="ENSBIXP00000033627.1"/>
        <property type="gene ID" value="ENSBIXG00000002244.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000037.1">
        <property type="protein sequence ID" value="ENSBIXP00005000413.1"/>
        <property type="gene ID" value="ENSBIXG00005030260.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000041.1">
        <property type="protein sequence ID" value="ENSBIXP00005000411.1"/>
        <property type="gene ID" value="ENSBIXG00005030258.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000049.1">
        <property type="protein sequence ID" value="ENSBIXP00005027688.1"/>
        <property type="gene ID" value="ENSBIXG00005030254.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000056.1">
        <property type="protein sequence ID" value="ENSBIXP00005027689.1"/>
        <property type="gene ID" value="ENSBIXG00005030250.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000062.1">
        <property type="protein sequence ID" value="ENSBIXP00005027694.1"/>
        <property type="gene ID" value="ENSBIXG00005030243.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000067.1">
        <property type="protein sequence ID" value="ENSBIXP00005000428.1"/>
        <property type="gene ID" value="ENSBIXG00005030240.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013228"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000025">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF23">
        <property type="entry name" value="TAFII28-LIKE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="3">
        <location>
            <begin position="92"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="193" mass="21452" checksum="D7FDAFA9AAA9E1F0" modified="2019-09-18" version="1">DVSREAPGVPLGPEPSNPGRILPRDPAQNARKASDEEGGELVHQDCPQPRAGEEADSSSRSPVAKRRKVDTEGKRGRQREVGEEEAQKMRTLVAAMSKEQLNRYEVFRRSAFPKSTIKHLIQAVAGTPVSQNVVIAMSGMAKVFVGEVVEEALDVCEKWGEKPPLQPKHLREAVRRLREKGQMPSTKHKILFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C0D0</accession>
    <name>A0A4W2C0D0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cleavage and polyadenylation specificity factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cleavage and polyadenylation specificity factor 160 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CPSF1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction. May play a role in eye morphogenesis and the development of retinal ganglion cell projections to the midbrain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CPSF1 family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2C0D0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004311.1">
        <property type="protein sequence ID" value="ENSBIXP00000006161.1"/>
        <property type="gene ID" value="ENSBIXG00000012294.1"/>
    </dbReference>
    <dbReference type="OMA" id="PMTKFKL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002223">
        <property type="entry name" value="Cleavage and polyadenylation specific factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001659">
        <property type="entry name" value="Cleavage and polyadenylation specific factor 1, 160kDa (Predicted), isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000118">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="12">
        <location>
            <begin position="85"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="13">
        <location>
            <begin position="591"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="11">
        <location>
            <begin position="1142"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="474"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="617"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="784"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="970"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="827"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1512" mass="167960" checksum="3488D637E4934B7F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C270</accession>
    <name>A0A4W2C270_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C270"/>
    <dbReference type="STRING" id="30522.A0A4W2C270"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053289.1">
        <property type="protein sequence ID" value="ENSBIXP00000000361.1"/>
        <property type="gene ID" value="ENSBIXG00000029915.1"/>
    </dbReference>
    <dbReference type="OMA" id="CIVIDVE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000349">
        <property type="entry name" value="Heat shock transcription factor, Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="9">
        <location>
            <begin position="71"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="199" mass="23339" checksum="BCD874B6AA14AF99" modified="2019-09-18" version="1">MAHIPSEIQDGPPKDESTDSEASIRSSLYDYTLTRDSVLRSMIEEYAFQALSEDLVIKRPCYKYSVSETDEVNNFLSLTFPQKLWNIVGSDQFESIWWDERGTCIVINEELFQKEVLERKAPFRIFETKTMKSLIQQLNLYGFSKKRQTFQRSDSRPVFLEEENNISLLSKVCQHFHLLAICKIKSCDPRKHVHICSYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2Q4</accession>
    <name>A0A4W2C2Q4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nuclear factor NF-kappa-B p100 subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">DNA-binding factor KBF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NFKB2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Component of the NF-kappa-B RelB-p52 complex. Homodimer; component of the NF-kappa-B p52-p52 complex. Component of the NF-kappa-B p65-p52 complex. Component of the NF-kappa-B p52-c-Rel complex. NFKB2/p52 interacts with NFKBIE. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Directly interacts with MEN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027385280.1">
        <property type="nucleotide sequence ID" value="XM_027529479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C2Q4"/>
    <dbReference type="SMR" id="A0A4W2C2Q4"/>
    <dbReference type="STRING" id="30522.A0A4W2C2Q4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004530.1">
        <property type="protein sequence ID" value="ENSBIXP00000006047.1"/>
        <property type="gene ID" value="ENSBIXG00000012188.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001895.1">
        <property type="protein sequence ID" value="ENSBIXP00005007670.1"/>
        <property type="gene ID" value="ENSBIXG00005012914.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884671"/>
    <dbReference type="CTD" id="4791"/>
    <dbReference type="GeneTree" id="ENSGT00940000160968"/>
    <dbReference type="OMA" id="QIAHIIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033257">
        <property type="term" value="C:Bcl3/NF-kappaB2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002268">
        <property type="term" value="P:follicular dendritic cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002467">
        <property type="term" value="P:germinal center formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048536">
        <property type="term" value="P:spleen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08798">
        <property type="entry name" value="Death_NFkB2_p100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07934">
        <property type="entry name" value="RHD-n_NFkB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000127">
        <property type="entry name" value="Nuclear factor NF-kappa-B p100 subunit isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000061">
        <property type="entry name" value="nuclear factor NF-kappa-B p100 subunit isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000004">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030497">
        <property type="entry name" value="NFkB_p100_RHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF21">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B P100 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="10 20" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="11" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RHD" evidence="23">
        <location>
            <begin position="35"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="20">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="20">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="20">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="20">
        <location>
            <begin position="668"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="405"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="699"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="849"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00084044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="900" mass="96964" checksum="2CC170A8D30984C4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6B4</accession>
    <name>A0A4W2C6B4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1</fullName>
            <ecNumber evidence="5">3.1.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">HD domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Metazoan SpoT homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Penta-phosphate guanosine-3'-pyrophosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HDDC3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">ppGpp hydrolyzing enzyme involved in starvation response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77828"/>
            <dbReference type="EC" id="3.1.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MESH1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.7.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6B4"/>
    <dbReference type="STRING" id="30522.A0A4W2C6B4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015968.1">
        <property type="protein sequence ID" value="ENSBIXP00000008329.1"/>
        <property type="gene ID" value="ENSBIXG00000014196.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="13">
        <location>
            <begin position="32"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024387"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="179" mass="20434" checksum="B54FBFD614950E2A" modified="2019-09-18" version="1">MGSEVAQLLEAADFAARKHQRQRRKDPEGTPYINHPIGVARILTHEAGITDIVVLQAALLHDTVEDTDTTLDEVELHFGDQVRRLVEEVTDNKTLPKLERKRLQVEHAPQSSPGAKLVKLADKLYNLRDLNRCTPEGWTEHRVQEYFEWAAQVVKGLQGTNRQLEEALRQLFKERGLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7E3</accession>
    <name>A0A4W2C7E3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Apolipoprotein B receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">APOBR</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027382509.1">
        <property type="nucleotide sequence ID" value="XM_027526708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7E3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012391.1">
        <property type="protein sequence ID" value="ENSBIXP00000002204.1"/>
        <property type="gene ID" value="ENSBIXG00000008687.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050089.1">
        <property type="protein sequence ID" value="ENSBIXP00005040485.1"/>
        <property type="gene ID" value="ENSBIXG00005023162.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883216"/>
    <dbReference type="CTD" id="55911"/>
    <dbReference type="GeneTree" id="ENSGT00530000065031"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030229">
        <property type="term" value="F:very-low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026158">
        <property type="entry name" value="ApolipoprotB_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15964:SF0">
        <property type="entry name" value="APOLIPOPROTEIN B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15964">
        <property type="entry name" value="APOLIPOPROTEIN B48 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="916"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1020" mass="108765" checksum="9FA57B38F31802FB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C868</accession>
    <name>A0A4W2C868_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AKT-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FHIP1B, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. Interacts directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Also interacts with AKT1. May interact with FHIP1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C868"/>
    <dbReference type="SMR" id="A0A4W2C868"/>
    <dbReference type="STRING" id="30522.A0A4W2C868"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017100.1">
        <property type="protein sequence ID" value="ENSBIXP00000009041.1"/>
        <property type="gene ID" value="ENSBIXG00000014781.1"/>
    </dbReference>
    <dbReference type="OMA" id="WGFPEWR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23814">
        <property type="entry name" value="UEV_AKTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000030">
        <property type="entry name" value="AKT-interacting protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="74"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="254"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="293" mass="33228" checksum="7F3300AF03D4356F" modified="2019-09-18" version="1">MNPFWSMSTSSVRKRSDGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPATQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDVPVFHPLVDPASGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8V7</accession>
    <name>A0A4W2C8V7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Polyunsaturated fatty acid lipoxygenase ALOX15</fullName>
            <ecNumber evidence="25">1.13.11.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">12/15-lipoxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Arachidonate 12-lipoxygenase, leukocyte-type</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Arachidonate 15-lipoxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Arachidonate omega-6 lipoxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Erythroid cell-specific 15-lipoxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Hepoxilin A3 synthase Alox15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Linoleate 13S-lipoxygenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="41 42">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="41 42" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="41" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = (8S)-hydroxy-(11S,12S)-epoxy-(5Z,9E,14Z)-eicosatrienoate</text>
            <dbReference type="Rhea" id="RHEA:50216"/>
            <dbReference type="ChEBI" id="CHEBI:57444"/>
            <dbReference type="ChEBI" id="CHEBI:132129"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:50217"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (5S)-hydroperoxy-(14R,15S)-epoxy-(6E,8Z,11Z)-eicosatrienoate</text>
            <dbReference type="Rhea" id="RHEA:50280"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:131965"/>
            <dbReference type="ChEBI" id="CHEBI:132067"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:50281"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (8S)-hydroperoxy-(14S,15R)-epoxy-(5Z,9E,11Z)-eicosatrienoate</text>
            <dbReference type="Rhea" id="RHEA:50288"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:131964"/>
            <dbReference type="ChEBI" id="CHEBI:132068"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50289"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(15R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:50152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
            <dbReference type="ChEBI" id="CHEBI:82626"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:50153"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = (14S,15S)-epoxy-(5Z,8Z,10E,12E)-eicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:50140"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57446"/>
            <dbReference type="ChEBI" id="CHEBI:132070"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:50141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate + O2 = 14-hydroperoxy-(4Z,7Z,10Z,12E,16Z)-docosapentaenoate</text>
            <dbReference type="Rhea" id="RHEA:50824"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:77226"/>
            <dbReference type="ChEBI" id="CHEBI:133799"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:50825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (11S)-hydroperoxy-(4Z,7Z,9E,13Z,16Z,19Z)-docosahexaenoate</text>
            <dbReference type="Rhea" id="RHEA:64732"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
            <dbReference type="ChEBI" id="CHEBI:156131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:64733"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = (14R,15S)-dihydroperoxy-(5Z,8Z,10E,12E)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:50928"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:133900"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:50929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = (8S,15S)-dihydroperoxy-(5Z,9E,11Z,13E)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:50924"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:133899"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:50925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + O2 = 14-hydroperoxy-(7Z,10Z,12E,16Z,19Z)-docosapentaenoate</text>
            <dbReference type="Rhea" id="RHEA:50836"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:77224"/>
            <dbReference type="ChEBI" id="CHEBI:133798"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:50837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate</text>
            <dbReference type="Rhea" id="RHEA:22780"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:57466"/>
            <dbReference type="EC" id="1.13.11.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:22781"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="34">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="34">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23">Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="24">Interacts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 38">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="37">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.12" evidence="25"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C8V7"/>
    <dbReference type="STRING" id="30522.A0A4W2C8V7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000635.1">
        <property type="protein sequence ID" value="ENSBIXP00000008011.1"/>
        <property type="gene ID" value="ENSBIXG00000013868.1"/>
    </dbReference>
    <dbReference type="OMA" id="TYPDEYF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004052">
        <property type="term" value="F:arachidonate 12(S)-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016165">
        <property type="term" value="F:linoleate 13S-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01753">
        <property type="entry name" value="PLAT_LOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000001">
        <property type="entry name" value="arachidonate 12-lipoxygenase, 12R-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000001">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
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    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042062">
        <property type="entry name" value="PLAT_LOX_verte"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="35" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 38" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9 34 38" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6 34 38" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 38" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="42" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="39">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="40">
        <location>
            <begin position="125"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="36">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00037897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00038731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00038988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00040850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00041403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00041853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00041889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00042534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00043163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00043170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00077387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="677" mass="76417" checksum="43F5EE57D4034158" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9V1</accession>
    <name>A0A4W2C9V1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartate aminotransferase</fullName>
            <ecNumber evidence="12">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GOT2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027372194.1">
        <property type="nucleotide sequence ID" value="XM_027516393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C9V1"/>
    <dbReference type="SMR" id="A0A4W2C9V1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018051.1">
        <property type="protein sequence ID" value="ENSBIXP00000009655.1"/>
        <property type="gene ID" value="ENSBIXG00000015299.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026323.1">
        <property type="protein sequence ID" value="ENSBIXP00005015462.1"/>
        <property type="gene ID" value="ENSBIXG00005005444.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876780"/>
    <dbReference type="CTD" id="2806"/>
    <dbReference type="GeneTree" id="ENSGT00950000183082"/>
    <dbReference type="OMA" id="VGACTIV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000026">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000160">
        <property type="entry name" value="Similar to aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0944">Nitration</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="13">
        <location>
            <begin position="58"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="430" mass="47514" checksum="16DDF475382035AA" modified="2019-09-18" version="1">MALLHSGRFLSGVAAAFHPGLAAAASARASSWWAHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIAGLAEFCKASAELALGENNEVLKSGRYVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQSYRYYDPKTCGFDFTGAIEDISKIPAQSVILLHACAHNPTGVDPRPEQWKEMATVVKKNNLFAFFDMAYQGFASGDGNKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTVVCKDAEEAKRVESQLKILIRPMYSNPPINGARIASTILTSPDLRKQWLHEVKGMADRIISMRTQLVSNLKKEGSSHNWQHIIDQIGMFCYTGLKPEQVERLTKEFSIYMTKDGRISVAGVTSGNVAYLAHAIHQVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAI5</accession>
    <name>A0A4W2CAI5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAI5"/>
    <dbReference type="STRING" id="30522.A0A4W2CAI5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018408.1">
        <property type="protein sequence ID" value="ENSBIXP00000009874.1"/>
        <property type="gene ID" value="ENSBIXG00000015495.1"/>
    </dbReference>
    <dbReference type="OMA" id="CFVNKEE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019370">
        <property type="entry name" value="E2F-assoc_phosphoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15967">
        <property type="entry name" value="E2F-ASSOCIATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15967:SF0">
        <property type="entry name" value="E2F-ASSOCIATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10238">
        <property type="entry name" value="Eapp_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="285" mass="32772" checksum="928D8E31B9D9D052" modified="2019-09-18" version="1">MNRLQDDYDPYAVEEPSDEEPALSSSEDEVDVLLHGTPDQKRKLIRECLTGESESSSEDEFEKEMEAELNSTIKTMEDKLSSLETGSSGDGIVGTAPTKYYDNIYFDSDSEDEDKAAQVTKKKKKKQHRIPTNDELLYDPEKDNRDQAWVDAQRRGYHGFGIQRPRQQQQPVPNSDAVLNCPACMTTLCLDCQRHESYKTQYRAMFVMNCSVNKEEILRYKPPENRKKRRGHKKMRSNQEGAAEEAETDVEEIYHPVMCTECSTEVAVYDKDEVFHFFNVLASHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCA9</accession>
    <name>A0A4W2CCA9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cell cycle checkpoint control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RAD9A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'-&gt;5' double stranded DNA exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the rad9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCA9"/>
    <dbReference type="SMR" id="A0A4W2CCA9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008821.1">
        <property type="protein sequence ID" value="ENSBIXP00000003962.1"/>
        <property type="gene ID" value="ENSBIXG00000000558.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000005">
        <property type="entry name" value="Cell cycle checkpoint control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026584">
        <property type="entry name" value="Rad9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF1">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN RAD9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04139">
        <property type="entry name" value="Rad9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009303">
        <property type="entry name" value="Cell_cycle_RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="315"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="395" mass="43128" checksum="8AC3A3E459214649" modified="2019-09-18" version="1">MKCLVTGSNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDQLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNDRSSRLVVQLHCKYGVRKTHNLSFQDCESLQAVFDPALCPHVLRAPARVLVEAVLPFPPALAEVTLGIGHGRRVILRSYQEEEADSAIKAMVTEMSIGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLSLSIHFDAPGRPAIFAIEDSLLDGHFVLATLSESDSHPQTLHAQEELQRPEPGPQAHSTPHLDLDDFAVDDMDSYMIAMETTTGSEGSRALPSISLSPGLQRPCSSSPHSEEEDDDMEPSTVPGTPPPKKFRSLFFGSILAPAHSPQGPSPVLAEDSEGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEH6</accession>
    <name>A0A4W2CEH6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">All trans-polyprenyl-diphosphate synthase PDSS1</fullName>
            <ecNumber evidence="16">2.5.1.91</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">All-trans-decaprenyl-diphosphate synthase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Decaprenyl-diphosphate synthase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Solanesyl-diphosphate synthase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Trans-prenyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">PDSS1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Heterotetrameric enzyme that catalyzes the condensation of farnesyl diphosphate (FPP), which acts as a primer, and isopentenyl diphosphate (IPP) to produce prenyl diphosphates of varying chain lengths and participates in the determination of the side chain of ubiquinone. Supplies nona and decaprenyl diphosphate, the precursors for the side chain of the isoprenoid quinones ubiquinone-9 (Q9)and ubiquinone-10 (Q10) respectively. The enzyme adds isopentenyl diphosphate molecules sequentially to farnesyl diphosphate with trans stereochemistry.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>6 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = all-trans-nonaprenyl diphosphate + 6 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:55364"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58391"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:55365"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>7 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = all-trans-decaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27802"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60721"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.91"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:27803"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterotetramer composed of 2 PDSS1/DPS1 and 2 PDSS2/DLP1 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.91" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEH6"/>
    <dbReference type="STRING" id="30522.A0A4W2CEH6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020628.1">
        <property type="protein sequence ID" value="ENSBIXP00000011320.1"/>
        <property type="gene ID" value="ENSBIXG00000016588.1"/>
    </dbReference>
    <dbReference type="OMA" id="GKQMRPM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032478">
        <property type="term" value="C:heterotetrameric polyprenyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097269">
        <property type="term" value="F:all-trans-decaprenyl-diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000011">
        <property type="entry name" value="Decaprenyl diphosphate synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00084036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="418" mass="46312" checksum="F921311DE0E12345" modified="2019-09-18" version="1">MASCWWPWRWRRGCSWRPAARSPGPNSAGRAALYAPRAAAVAAAATVAVARAQMSYCNLMKSLTSAFRNMYGVSRFHHTTPVACCFSETLSGEKYTDPFKIGWRDLKGLYEDIRKELFISTAELREMSEYHFDGKGKAFRPIIVVLMARACNIHHSNARNVQASQRAIALIAEMIHTASLVHDDIIDDASSRRGKHTVNKIWGEKKAVLAGDLILSAASIALARIGNTTVISILTQVIEDLVRGEFLQLGSKENENERFAHYLEKTFKKTASLIANSCKAVSVLGCPDPAVHEIAYQYGKNVGIAFQLIDDVLDFTSCSEQMGKPTSADLKLGLATGPVLFACQQFPEMNAMIMRRFGSPGDVDRALQYVLQSDGVQQTTYLAQRYCHKAVSEISKLRPSPERDALIQLSEMVLTRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHL6</accession>
    <name>A0A4W2CHL6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Beta-1,4-N-acetyl-galactosaminyltransferase 2 (SID blood group)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">B4GALNT2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027373954.1">
        <property type="nucleotide sequence ID" value="XM_027518153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHL6"/>
    <dbReference type="STRING" id="30522.A0A4W2CHL6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005050.1">
        <property type="protein sequence ID" value="ENSBIXP00000005795.1"/>
        <property type="gene ID" value="ENSBIXG00000011922.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014239.1">
        <property type="protein sequence ID" value="ENSBIXP00005001442.1"/>
        <property type="gene ID" value="ENSBIXG00005007929.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877740"/>
    <dbReference type="CTD" id="124872"/>
    <dbReference type="GeneTree" id="ENSGT00390000006679"/>
    <dbReference type="OMA" id="GDCIHRR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008376">
        <property type="term" value="F:acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022408">
        <property type="term" value="P:negative regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098528">
        <property type="term" value="P:skeletal muscle fiber differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043403">
        <property type="term" value="P:skeletal muscle tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019276">
        <property type="term" value="P:UDP-N-acetylgalactosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011143">
        <property type="entry name" value="GM2_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15046:SF2">
        <property type="entry name" value="BETA-1,4 N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15046">
        <property type="entry name" value="GLYCO_TRANS_2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000474">
        <property type="entry name" value="GM2_GD2_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="12">
        <location>
            <begin position="265"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="507" mass="57815" checksum="77C91161A5B268E8" modified="2019-09-18" version="1">MMTSFGSRYLWILKMSVLFLALGIVLFMFRSVYLYSEFSSYKAPLLQPSPAAGMLKLLPEEHIKTLFTHDGIWLFPKNQCKCEAWIHRQRYNFENAYDQTDLPAVKMRRQAEFEHFQRREGLPRPPPLLAQANLPFGYPVHGVEVMPLHTIPIPGLQFEGPDAPIYKVTLKASLGTLNTLADTPDSVVQGRGQKQLTILTSSRKVLNFILQHVTYTSTEYQHHRVDVVSLESNSSVAKFPVTIRFPVMPKLYDPGPERKLRNLVSIATKTFLRPHKLMTMLQSIREYYPDLTVIVADDSKEPLKINDSHVEYYTMPYGKGWFAGRNLAISQVTTKYVLWVDDDFLFNDKTKIEVLVDVLEKTELDVVGGSVLGNMFQFKLFLEHSKNGDCLHRRTGSFGPLDGFPNCVVTSGVVNFFLAHTERLQRVGFDPRLQRVAHSEFFIDGLGSLLVGSCSDVIIGHQPHSPVEDPELAALEKTYRIYRANTNDKIQFKLALHYFKNHLQCAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMI4</accession>
    <name>A0A4W2CMI4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger SWIM-type containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ZSWIM3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027414207.1">
        <property type="nucleotide sequence ID" value="XM_027558406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMI4"/>
    <dbReference type="STRING" id="30522.A0A4W2CMI4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046482.1">
        <property type="protein sequence ID" value="ENSBIXP00000014333.1"/>
        <property type="gene ID" value="ENSBIXG00000019051.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046255.1">
        <property type="protein sequence ID" value="ENSBIXP00005012618.1"/>
        <property type="gene ID" value="ENSBIXG00005016824.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902960"/>
    <dbReference type="CTD" id="140831"/>
    <dbReference type="GeneTree" id="ENSGT00390000017273"/>
    <dbReference type="OMA" id="LNTQHVH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052579">
        <property type="entry name" value="Zinc_finger_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048326">
        <property type="entry name" value="ZSWIM1-3_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048324">
        <property type="entry name" value="ZSWIM1-3_RNaseH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045563">
        <property type="entry name" value="ZSWIM1/3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048325">
        <property type="entry name" value="ZSWIM3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31569">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31569:SF3">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19286">
        <property type="entry name" value="ZSWIM1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21600">
        <property type="entry name" value="ZSWIM1-3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21056">
        <property type="entry name" value="ZSWIM1-3_RNaseH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21599">
        <property type="entry name" value="ZSWIM3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="526"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="692" mass="77986" checksum="E70C24E69BD00D5F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CN83</accession>
    <name>A0A4W2CN83_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)H oxidase (H2O2-forming)</fullName>
            <ecNumber evidence="5">1.6.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>NADH + O2 + H(+) = H2O2 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:11264"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NADPH + O2 + H(+) = H2O2 + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:11260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.6.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis; thyroid hormone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the peroxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.3.1" evidence="5"/>
    <dbReference type="SMR" id="A0A4W2CN83"/>
    <dbReference type="STRING" id="30522.A0A4W2CN83"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025604.1">
        <property type="protein sequence ID" value="ENSBIXP00000014629.1"/>
        <property type="gene ID" value="ENSBIXG00000019287.1"/>
    </dbReference>
    <dbReference type="OMA" id="NASMLHP"/>
    <dbReference type="UniPathway" id="UPA00194"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09820">
        <property type="entry name" value="dual_peroxidase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000043">
        <property type="entry name" value="dual oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000095">
        <property type="entry name" value="dual oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000004">
        <property type="entry name" value="Dual oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000006">
        <property type="entry name" value="Dual oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034821">
        <property type="entry name" value="DUOX_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF75">
        <property type="entry name" value="DUAL OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
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        <source>
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        <source>
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        </source>
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    <sequence length="1553" mass="177622" checksum="1562E97C1E8D903F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CNJ3</accession>
    <name>A0A4W2CNJ3_BOBOX</name>
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        <recommendedName>
            <fullName evidence="16">Ganglioside-induced differentiation-associated protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="21" type="primary">GDAP1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="15">Regulates the mitochondrial network by promoting mitochondrial fission.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
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    </dbReference>
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        <property type="entry name" value="GST_NTER"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="GST N-terminal" evidence="19">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="20">
        <location>
            <begin position="153"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="164"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="358" mass="41202" checksum="01806D1D09891F23" modified="2019-09-18" version="1">MARRQDEQRGGAPLIAEGKSDAEVKLILYHWTHSFSSQKVRLVIAEKALKCEEHDVSLPLSEHNEPWFMRLNSTGEVPVLIHGENIICEATQIIDYLEQTFLDEKTPRLMPDKGSMYYPRVQHYRELLDSLPMDAYTHGCILHPELTVDSMIPAYATTRIRSQIGNTESELKKLAEENPDLQEAYIAKQKRLKSKLLDHDNVKYLKKILDELEKVLDQVETELQRRNEETPEEGRQPWLCGESFTLADVSLAVTLHRLKFLGFARRNWGNGKRPNLETYYERVLKRKTFNKVLGHVNNILISAVLPTAFRVAKKRAPKVLGTTLVVGLLAGMGYFAFMLFRKRLGSMILALRPRPNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPS1</accession>
    <name>A0A4W2CPS1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CPS1"/>
    <dbReference type="STRING" id="30522.A0A4W2CPS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046275.1">
        <property type="protein sequence ID" value="ENSBIXP00000013948.1"/>
        <property type="gene ID" value="ENSBIXG00000018772.1"/>
    </dbReference>
    <dbReference type="OMA" id="NHRICAR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042335">
        <property type="entry name" value="ANKRD53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24160">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24160:SF1">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="118"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="482" mass="54231" checksum="A4C9D83345737C1C" modified="2019-09-18" version="1">VTWPGRRAVPAPPPPPAGTSSGGRRRRHPSGGGGTSPGTYTSEEWDQRAMGRSYYQLFAASLGNLEWLRFCLNRHRGEILADDKGFTAIHFAAQSGKLACLQVLVEEYKFPVNLPTNNGQTPLHLVIHGDNKTVALACIHYLIKQGAALNTQTCNGSTPLHLAAREGLLSCVKVLVQKGANVHAQDATGCKPIDYCKIWNHRVCARFLKDAMWKRDKEDFAHEMRKLKRLKDQLALMEEDYLTEYQKEHQILREADFKKWLHHKLLLQDQSLIPSVQREPRAPPRATALSKTCKDGVLWPPTSFHPSPEARLQQIQQPNLPLVMSVPTYKPPTIRRPKVWNLSSNPTRSPTAQIGYPQGVRLGVHPDPRPERDFRCFVEVRSDGHGGAHLRTVAGRQVAPVPRLPFEVVIRELHPSVRPYRMKVPQDFCSVAMEDVPRKRHLGDSSFWTDTLSMNLRETFDEAFLTPDPLLSTKESPPGDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSD3</accession>
    <name>A0A4W2CSD3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PAN2-PAN3 deadenylation complex catalytic subunit PAN2</fullName>
            <ecNumber evidence="13">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Inactive ubiquitin carboxyl-terminal hydrolase 52</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Poly(A)-nuclease deadenylation complex subunit 2</fullName>
            <shortName evidence="13">PAN deadenylation complex subunit 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">PAN2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="13">Binds 2 metal cations per subunit in the catalytic exonuclease domain.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Positively regulated by the regulatory subunit PAN3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts with PAN3 isoform 1/Pan3L and isoform 3/Pan3S. Interacts with ZFP36.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts with ZFP36.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
            <location evidence="13">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <text evidence="13">Shuttles between nucleus and cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peptidase C19 family. PAN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSD3"/>
    <dbReference type="STRING" id="30522.A0A4W2CSD3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026021.1">
        <property type="protein sequence ID" value="ENSBIXP00000014876.1"/>
        <property type="gene ID" value="ENSBIXG00000019481.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017660.1">
        <property type="protein sequence ID" value="ENSBIXP00005009599.1"/>
        <property type="gene ID" value="ENSBIXG00005014495.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013978"/>
    <dbReference type="OMA" id="TQELLWT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02672">
        <property type="entry name" value="Peptidase_C19P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000059">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000011">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000017">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03182">
        <property type="entry name" value="PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030843">
        <property type="entry name" value="PAN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="520"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="981"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="983"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="1090"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="1142"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1205" mass="135802" checksum="8A6529FC63146403" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUX1</accession>
    <name>A0A4W2CUX1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CUX1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029508.1">
        <property type="protein sequence ID" value="ENSBIXP00000017174.1"/>
        <property type="gene ID" value="ENSBIXG00000021198.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="364" mass="41788" checksum="E51D48D3D5FA3B54" modified="2019-09-18" version="1">MGKTRDLFKKIRDIKGTFHAKMGLTKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITHLEPDILECEVKWALESITMNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKHNAKECSHYLTIALISHTSKVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANIRWIMEKATEFQQNIYFCIIDYAKAFDCVDHNQLWKILKEMGIPDHLTYLLRNLEAGQEATVRTGHGVTDWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNTNNLRYADDTTLMAESQEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW48</accession>
    <name>A0A4W2CW48_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger and BTB domain-containing protein 43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Zinc finger protein 297B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ZBTB43</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with BDP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027412093.1">
        <property type="nucleotide sequence ID" value="XM_027556292.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027412094.1">
        <property type="nucleotide sequence ID" value="XM_027556293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW48"/>
    <dbReference type="SMR" id="A0A4W2CW48"/>
    <dbReference type="STRING" id="30522.A0A4W2CW48"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027810.1">
        <property type="protein sequence ID" value="ENSBIXP00000016076.1"/>
        <property type="gene ID" value="ENSBIXG00000020360.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046344.1">
        <property type="protein sequence ID" value="ENSBIXP00005012785.1"/>
        <property type="gene ID" value="ENSBIXG00005016956.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901656"/>
    <dbReference type="CTD" id="23099"/>
    <dbReference type="GeneTree" id="ENSGT00940000160616"/>
    <dbReference type="OMA" id="IEMVAGI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001025">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18227">
        <property type="entry name" value="BTB_POZ_ZBTB43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000009">
        <property type="entry name" value="Zinc finger and BTB domain-containing 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000808">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF15">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="21">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="428"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="162"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="498" mass="56202" checksum="51C5AF974FA1FBBD" modified="2019-09-18" version="1">MEPGTNSFRVEFPDFSSTILQKLNQQRQQGQLCDVSIVVQGHIFRAHKAVLAASSPYFCDQVLLKNSRRIVLPDVMNPRVFENILLSSYTGRLVMPAPEIVSYLTAASFLQMWHVVDKCTEVLEGNPTVLCQKLNHGSDHQSPSSSNYNGLVESFELGSGGHTDFPKAQELRDGENEEESTKDELSSQLTEHEYLPSNSSTEHDRLSTEMASQDGEEGASDSAEFHYTRPMYSKPSIMAHKRWIHVKPERFEQACEGMDVHAPYDEHQVTESINTVQTEYSVQPSGVEEDFHIGEKKVEAEFDEQADESNYDEQVDFYGSSMEEFSGERSDGNLIWHKQEAALASGYSENIEMVTGIKEEASHLGFSATDKLYLCQCGKSFTHKSQRDRHMSMHLGLRPYGCGVCGKKFKMKHHLVGHMKIHTGIKPYECNICAKRFMWRDSFHRHVTSCTKSYEAAKAEQNTTEACTKSYEAAKAEQNTTETCTKSYEAAKAEQNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXC4</accession>
    <name>A0A4W2CXC4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tripartite motif-containing protein 54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">TRIM54</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May bind and stabilize microtubules during myotubes formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027411623.1">
        <property type="nucleotide sequence ID" value="XM_027555822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXC4"/>
    <dbReference type="SMR" id="A0A4W2CXC4"/>
    <dbReference type="STRING" id="30522.A0A4W2CXC4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030955.1">
        <property type="protein sequence ID" value="ENSBIXP00000018106.1"/>
        <property type="gene ID" value="ENSBIXG00000021995.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043301.1">
        <property type="protein sequence ID" value="ENSBIXP00005045226.1"/>
        <property type="gene ID" value="ENSBIXG00005005109.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901453"/>
    <dbReference type="CTD" id="57159"/>
    <dbReference type="GeneTree" id="ENSGT00940000156529"/>
    <dbReference type="OMA" id="MEDICRT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19833">
        <property type="entry name" value="Bbox2_MuRF3_C-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16761">
        <property type="entry name" value="RING-HC_MuRF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000014">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase MID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000022">
        <property type="entry name" value="Tripartite motif containing 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000140">
        <property type="entry name" value="Tripartite motif containing 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033492">
        <property type="entry name" value="Trim54_Bbox2_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042752">
        <property type="entry name" value="TRIM54_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="18">
        <location>
            <begin position="121"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="19">
        <location>
            <begin position="271"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="326"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="185"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="366" mass="41337" checksum="9058297044439497" modified="2019-09-18" version="1">MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRSSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEDEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEENSRRQKQLLNQRFEGLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGTMSKVELAGRPEPGYERMDQFTVSVEHVAEMLRTIDFQPGTSGEEEDEEVAVEGEEGNAGPEEERTDGRESTGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXW9</accession>
    <name>A0A4W2CXW9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatase and actin regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Binds PPP1CA and actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXW9"/>
    <dbReference type="STRING" id="30522.A0A4W2CXW9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028773.1">
        <property type="protein sequence ID" value="ENSBIXP00000016699.1"/>
        <property type="gene ID" value="ENSBIXG00000020815.1"/>
    </dbReference>
    <dbReference type="OMA" id="GPSHKGD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1750">
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="582"/>
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        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060725"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073391"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="687" mass="77466" checksum="AE21AE947266A287" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZG0</accession>
    <name>A0A4W2CZG0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
            <ecNumber evidence="13">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ARMT1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism. Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues. Possibly methylates PCNA, suggesting it is involved in the DNA damage response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="13">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="EC" id="3.1.3.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027407660.1">
        <property type="nucleotide sequence ID" value="XM_027551859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZG0"/>
    <dbReference type="STRING" id="30522.A0A4W2CZG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029761.1">
        <property type="protein sequence ID" value="ENSBIXP00000017345.1"/>
        <property type="gene ID" value="ENSBIXG00000007204.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048566.1">
        <property type="protein sequence ID" value="ENSBIXP00005017120.1"/>
        <property type="gene ID" value="ENSBIXG00005020618.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898585"/>
    <dbReference type="CTD" id="79624"/>
    <dbReference type="GeneTree" id="ENSGT00530000064023"/>
    <dbReference type="OMA" id="IFARQKM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051998">
        <property type="term" value="F:protein carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10880:FF:000002">
        <property type="entry name" value="Acidic residue methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.60:FF:000001">
        <property type="entry name" value="protein-glutamate O-methyltransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="14">
        <location>
            <begin position="21"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="441" mass="50930" checksum="DB2B2638A4F7D069" modified="2019-09-18" version="1">MAGPPASLSARDVGSFAYLSVKDRSPQILTKAIDTLHRHKSEFFEKHGEKGLEAEKKAISLLSKLRNELQTDKPIVPLVEKFVDTDIWNQYLEYQQSLLNESDGKPRWFLSPWLFVECYMYRRIHEAIIQSPPIDDFDIFKELKDQNFFESQESIIALCTHLQELRKTIEDLDENQLKNEFFKVLQISLWGNKCDLSLSGGEHISQKTNIMNSLEDLKPFILVNDMDRLWSLLSNCKKTREKESVTRVDIVLDNSGFELITDLVLADFLLSSKLATKIHFYGKTIPWFVSDTTLHDFNWIIKQLKHSNNKWVSQCGVDWEDHVKTGRWVYLDHIFWTLPHEFSAMSQVAPDLHAALQKAHLIFFKGDLNYRKLTGDRRWEFTVPFHEALSGFHPAPLCSIRTLKAEVQVGLQPGQGEQLTASEPNWLTAGKYGVFQFDGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1I1</accession>
    <name>A0A4W2D1I1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DBIRD complex subunit ZNF326</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1I1"/>
    <dbReference type="STRING" id="30522.A0A4W2D1I1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022445.1">
        <property type="protein sequence ID" value="ENSBIXP00000012503.1"/>
        <property type="gene ID" value="ENSBIXG00000017564.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7997" checksum="2B14DA9707D29F80" modified="2019-09-18" version="1">MDFEDDYTHSACRNTYQSFNGMDRDYGPGSYRGMDRDYGHGSYGGQRSMDSYLNQSYGMDNHSGGGGRGSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D214</accession>
    <name>A0A4W2D214_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D214"/>
    <dbReference type="STRING" id="30522.A0A4W2D214"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033608.1">
        <property type="protein sequence ID" value="ENSBIXP00000019814.1"/>
        <property type="gene ID" value="ENSBIXG00000023337.1"/>
    </dbReference>
    <dbReference type="OMA" id="TMEWYRR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="201"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="203"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="347" mass="35953" checksum="523C84AD7303698A" modified="2019-09-18" version="1">MPDELTEPGRATPARASSFLIENLLAAEAKGAGSGAQGGGGREEEEDDDDDPEDEDAERARRRRLQRRRQLRAGSGPSAEARARPLLGPGALGLGPRPPPGPGPPYALGCGGAARWYSRSHGGYGGGLSPDTSDRDSPETGEEMGRAEGAWPRGSGPRAVQREAAELAARGPAAGAEEAAELAEAPVATAAAGEARGGGGGRKKKTRTVFSRSQVFQLESTFDLKRYLSSAERAGLAASLQLTETQVKIWFQNRRNKWKRQLAAELEAASLSPPGTQRLVRVPVLYHESPPAAGAAAAAALPFPLAPAAPAPPPPLLGFSGALAYPLAAFPAAASAPFLRAQMPGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2J4</accession>
    <name>A0A4W2D2J4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D2J4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049211.1">
        <property type="protein sequence ID" value="ENSBIXP00000020012.1"/>
        <property type="gene ID" value="ENSBIXG00000004634.1"/>
    </dbReference>
    <dbReference type="OMA" id="ITAHGDC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="138"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="412" mass="47350" checksum="388F29C18D536B0C" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQAGFRKGRGTRHQIANICWIIEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTGLLRNLYAGQEATVRTGHGTTDWLQIGKGVCQGCILSPCLFNLYAEYIMRNPGLEEAQAGIKIAERNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWEIDGKTVETVSDFILGDSKITAHGDCSHEIKRRLLLARKVMTNLDSILKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGCSLEGLMLKLKLQYFGHLMRRVDSLEKTLMLGGIGGRRRRGQQRMRWLDGIIDLMDVSLSELRELVMDREAWRAAIHGAAKSRTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3S8</accession>
    <name>A0A4W2D3S8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D3S8"/>
    <dbReference type="STRING" id="30522.A0A4W2D3S8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034581.1">
        <property type="protein sequence ID" value="ENSBIXP00000020482.1"/>
        <property type="gene ID" value="ENSBIXG00000023792.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTGWRKP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000016">
        <property type="entry name" value="B-cell linker protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF2">
        <property type="entry name" value="CYTOKINE-DEPENDENT HEMATOPOIETIC CELL LINKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="307"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="426" mass="48563" checksum="2603D15A44DF22C4" modified="2019-09-18" version="1">MNRPGNRKIAKEGASDLKFQNFALLKNRSWPRIDSATGQYQTINEPLLLDGERNSMAVLDGAKGHTGDDYEDPELHVGEDWQSLKILPARPIKESEYADTRYFKGMMDTPLSLDSKTSLPTDRLTWMKLEEVEKPISKDIRKQHVKGDKPTKGNKTPLPPPRPPVKPPKKYQPLPPEPPSSRPPFPQRHTFPEAQKGARQISLKNLSEVLGTEKVPYHQMKPEAPHLPQNQSPQEIPLAVASSSFMTRNPTVQNRDHKESMPSYAPQRCQSPAGSGPQENPLLHKITSRKKPFPRRSDGKDVQHNEWYIGEYSRQAVEEALMKENKDGTFLVRDCSAKSRAEPYVLVVFYGNKVYNVKIRFLERNQQFALGTGLRGDEKFNSVEAIIEHYKYFPIILIDGKDKTGTHREQCYLTQALPVNRHFSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D5C7</accession>
    <name>A0A4W2D5C7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SRA stem-loop-interacting RNA-binding protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SLIRP</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D5C7"/>
    <dbReference type="STRING" id="30522.A0A4W2D5C7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033001.1">
        <property type="protein sequence ID" value="ENSBIXP00000019421.1"/>
        <property type="gene ID" value="ENSBIXG00000004028.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050061.1">
        <property type="protein sequence ID" value="ENSBIXP00005040444.1"/>
        <property type="gene ID" value="ENSBIXG00005023113.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008624"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097222">
        <property type="term" value="P:mitochondrial mRNA polyadenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905638">
        <property type="term" value="P:negative regulation of mitochondrial mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12242">
        <property type="entry name" value="RRM_SLIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000392">
        <property type="entry name" value="SRA stem-loop-interacting RNA-binding protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034152">
        <property type="entry name" value="SLIRP_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF61">
        <property type="entry name" value="SRA STEM-LOOP INTERACTING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="19"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="117" mass="13321" checksum="FD016CC8DC56181D" modified="2019-09-18" version="1">MAASAARGAMALRTNIGRPVAFVRKIPWTAASSELREHFAQFGHVRKCTVPFDKETGFHKGMGWIHFSSEEELHNALQQENHVIDGVKESWFRTETEERFRRKNSQAVSLARSTPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7V7</accession>
    <name>A0A4W2D7V7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7V7"/>
    <dbReference type="STRING" id="30522.A0A4W2D7V7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034331.1">
        <property type="protein sequence ID" value="ENSBIXP00000020314.1"/>
        <property type="gene ID" value="ENSBIXG00000023646.1"/>
    </dbReference>
    <dbReference type="OMA" id="QVMLNQH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06950">
        <property type="entry name" value="NR_LBD_Tlx_PNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000082">
        <property type="entry name" value="NR2E3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000022">
        <property type="entry name" value="Nuclear receptor subfamily 2 group E member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="11">
        <location>
            <begin position="81"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="322" mass="35571" checksum="1A9C2AB109106AAD" modified="2019-09-18" version="1">MCPVDKAHRNQCQACRLKKCLQAGMNQDAVQNERQPRSTAQVRMDSVESETEPRLQPLATPPALAGPSSRGPTPVSAARALGHHFMASLITAETCTKLEPEDADENIDVTSNDPEFPSSPYSSSSPCALDSIHETSARLLFMAVKWAKNLPVFSNLPFRDQVILLEEAWSELFLLGAIQWSLPLDSCPLLALPEASAGGSSQGRLVLASAETRILQETISRFRALAVDPTEFACMKALVLFKPETRGLKDPEHVEALQDQSQVMLSQHSKAHHPSQPVRFGKLLLLLPSLRFISSERVELLFFRKTIGNTPMEKLLCDMFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA16</accession>
    <name>A0A4W2DA16_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FMO3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N,N-dimethylaniline + NADPH + O2 + H(+) = N,N-dimethylaniline N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:24468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16269"/>
            <dbReference type="ChEBI" id="CHEBI:17735"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 18">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA16"/>
    <dbReference type="SMR" id="A0A4W2DA16"/>
    <dbReference type="STRING" id="30522.A0A4W2DA16"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027088.1">
        <property type="protein sequence ID" value="ENSBIXP00000015585.1"/>
        <property type="gene ID" value="ENSBIXG00000020049.1"/>
    </dbReference>
    <dbReference type="OMA" id="WFGQSHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000023">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000042">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000073">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000279">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002255">
        <property type="entry name" value="Flavin_mOase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01123">
        <property type="entry name" value="FMOXYGENASE3"/>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="9 17 18" id="KW-0274">FAD</keyword>
    <keyword evidence="6 17 18" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="11 17" id="KW-0521">NADP</keyword>
    <keyword evidence="13 17 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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            <dbReference type="ARBA" id="ARBA00004389"/>
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            <dbReference type="ARBA" id="ARBA00012850"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="532" mass="60666" checksum="5EF6750B9AB70D59" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBK7</accession>
    <name>A0A4W2DBK7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 17</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBK7"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="11802" checksum="B8B8FB6175AC7AB1" modified="2019-09-18" version="1">MASHSREPGLLPCGSCDMVFRSWALLATHTQRFCIGRQTREVMIGAQPPVATEPRVPGVPNSSSPEASPLTPTTAFVDPPPPEDATSRLGCREEGWALPHSFDQPPLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDV2</accession>
    <name>A0A4W2DDV2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DDV2"/>
    <dbReference type="STRING" id="30522.A0A4W2DDV2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040263.1">
        <property type="protein sequence ID" value="ENSBIXP00000024249.1"/>
        <property type="gene ID" value="ENSBIXG00000005551.1"/>
    </dbReference>
    <dbReference type="OMA" id="IMNLAPY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070778">
        <property type="term" value="P:L-aspartate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070633">
        <property type="term" value="P:transepithelial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150104">
        <property type="term" value="P:transport across blood-brain barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3860.10:FF:000002">
        <property type="entry name" value="Amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF22">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0769">Symport</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="560" mass="60247" checksum="CFC2B0C8847F1135" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEX7</accession>
    <name>A0A4W2DEX7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GRPEL2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEX7"/>
    <dbReference type="SMR" id="A0A4W2DEX7"/>
    <dbReference type="STRING" id="30522.A0A4W2DEX7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040959.1">
        <property type="protein sequence ID" value="ENSBIXP00000024670.1"/>
        <property type="gene ID" value="ENSBIXG00000006151.1"/>
    </dbReference>
    <dbReference type="OMA" id="KQAHNDA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000003">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000004">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF10">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="224" mass="25120" checksum="C7DF70D99C8EF0C5" modified="2019-09-18" version="1">MAARLLWAVRRRMQPLAAHAASEGRGWLHPFSTATQRTAGEDCNSEDPPDELGPSLAERALKLKAVKLEKEVQDLTVRYQRAVADSENIRRRTQRCVEDAKIFGIQSFCKDLVEVADILEKTTECISEETEPADQKLTLEKIFRGLSLLEAKLKSVFAKHGLEKMTPIGDKYDPHEHELICHVPAGVGVQPGTVAFVRQDGYKLHGRTIRLAQVEVAVESQRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DGD5</accession>
    <name>A0A4W2DGD5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DGD5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041752.1">
        <property type="protein sequence ID" value="ENSBIXP00000025193.1"/>
        <property type="gene ID" value="ENSBIXG00000007836.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032781.1">
        <property type="protein sequence ID" value="ENSBIXP00005019760.1"/>
        <property type="gene ID" value="ENSBIXG00005022922.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183345"/>
    <dbReference type="OMA" id="ACHFTPT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="71" mass="7872" checksum="B670D06B72D60945" modified="2019-09-18" version="1">MFGVNVAKSSLHLHFLKTLEFRTDVSVTSMPGVAKDIRACHFTPTILHGDPRSDPVRSPHVKVVKFCSVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DHD9</accession>
    <name>A0A4W2DHD9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA repair and recombination protein RAD54-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RAD54 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RAD54L</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homohexamer. Interacts (via N-terminus) with RAD51. Interacts with NAP1L1. Interacts with BRD9; this interaction orchestrates RAD51-RAD54 complex formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DHD9"/>
    <dbReference type="STRING" id="30522.A0A4W2DHD9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039177.1">
        <property type="protein sequence ID" value="ENSBIXP00000023548.1"/>
        <property type="gene ID" value="ENSBIXG00000001808.1"/>
    </dbReference>
    <dbReference type="OMA" id="YTEHERM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18067">
        <property type="entry name" value="DEXHc_RAD54A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.850:FF:000002">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000010">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000332">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="171"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="501"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="749" mass="84750" checksum="9AEE2D4B6996D043" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJT1</accession>
    <name>A0A4W2DJT1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJT1"/>
    <dbReference type="STRING" id="30522.A0A4W2DJT1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048817.1">
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        <property type="gene ID" value="ENSBIXG00000022808.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014003">
        <property type="term" value="P:oligodendrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052296">
        <property type="entry name" value="TR-Histone_Methyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="AGAP007109-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16516:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN 488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="234" mass="25433" checksum="7C65B4EBF9DAC8A6" modified="2019-09-18" version="1">LKKRLGGGQAQDRKHSGPVGQPGPQQLTPDIRRDLTGSTRVSVWPSGARREQRSAFSKPARGPAGRLGPACVFQAGEPADALGELLGLISTVDVTCWGRLSNSKLLVGDFWNLQTGPPHAPLCGTFLGAPTLWLKHATVQIPTPPSSSSTSSWALLPPTLTSLGLSTQNWCANCNLSFRLTSDLVFHMRSHHKKEHAGPSLHSKRLRAEALTCPICHECFRERHHLSRHMTSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQ65</accession>
    <name>A0A4W2DQ65_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LIMD1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQ65"/>
    <dbReference type="STRING" id="30522.A0A4W2DQ65"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010323.1">
        <property type="protein sequence ID" value="ENSBIXP00000028427.1"/>
        <property type="gene ID" value="ENSBIXG00000006923.1"/>
    </dbReference>
    <dbReference type="OMA" id="SCKEGPP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09352">
        <property type="entry name" value="LIM1_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09355">
        <property type="entry name" value="LIM2_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09438">
        <property type="entry name" value="LIM3_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000028">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000036">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000037">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047172">
        <property type="entry name" value="Ajuba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047245">
        <property type="entry name" value="Ajuba-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047247">
        <property type="entry name" value="Ajuba-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047248">
        <property type="entry name" value="Ajuba-like_LIM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219:SF3">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN JUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="19">
        <location>
            <begin position="468"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="19">
        <location>
            <begin position="533"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="19">
        <location>
            <begin position="594"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="73"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="142"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="99" mass="10734" checksum="CF61C1B623299204" modified="2019-09-18" version="1">MDLIGFGYAALVTFGSILGYKRRGGVLSLIAGLFVGFLAGYGAYRVSNDKRDVKLSLFTAFFLATIMGVRFKRSKKIMPAGLVAGLSLLMILRLVLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZI3</accession>
    <name>A0A4W2DZI3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZI3"/>
    <dbReference type="STRING" id="30522.A0A4W2DZI3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041076.1">
        <property type="protein sequence ID" value="ENSBIXP00000024750.1"/>
        <property type="gene ID" value="ENSBIXG00000026884.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027584.1">
        <property type="protein sequence ID" value="ENSBIXP00005037980.1"/>
        <property type="gene ID" value="ENSBIXG00005020010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161841"/>
    <dbReference type="OMA" id="DAFLPWR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000168">
        <property type="entry name" value="F-box only protein 6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007397">
        <property type="entry name" value="F-box-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039752">
        <property type="entry name" value="F-box_only"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04300">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01198">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51114">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="25"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="FBA" evidence="3">
        <location>
            <begin position="104"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32843" checksum="ED517BB669DBFAD2" modified="2019-09-18" version="1">MTTGASASQGLPACVPASDPEPKEAPGLSQLPIEMLLEMLSYLPTSMLLGQCRHVCWYWRYLVDTRAVWLSILPYSHVKLWPVFQTCLPLDNDDNSRPCLLGRFCERRPLGRNLYPNPHGIDAFLPWRTINCTSWREEESWEEDHRLVPEDSFQPFYRWCYKKQALDLEKEGLWPELLDSGKIEICVSAWWANQEASVCLYELIVQLLNAKQAKLHHFSPRPIHQGTTSIPFKTNHVFSNLKKGVCFVSLEHWIWDMGFLPEDYGIIVPKASVIVRVYWLLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3F5</accession>
    <name>A0A4W2E3F5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hexosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="11">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E3F5"/>
    <dbReference type="STRING" id="30522.A0A4W2E3F5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020651.1">
        <property type="protein sequence ID" value="ENSBIXP00000033784.1"/>
        <property type="gene ID" value="ENSBIXG00000002590.1"/>
    </dbReference>
    <dbReference type="OMA" id="MWKALHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000009">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF382">
        <property type="entry name" value="ACETYLGALACTOSAMINYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="458" mass="51254" checksum="9C59CCB57FAA7640" modified="2019-09-18" version="1">MTFSLIIKGKQAITVGRKLVGMSPTSTPLIQGKRDARIGQVHKHSLGGGASDCEQKNRVYGRSKSGDDSCLFLLSQTFKMAFPCRKFLKPKILTCLLAGLSFSILHLWFLRDPRFQQKQRSDGSLQVTHVAPAATSPSSPGPPCVANASANATADFEKLPARIQDFLRYRHCRHFPLLWDAPAKCRGSRGPFLLLAVKSAPANFERRELIRRTWGQERSYGGRPVRRVFLLGTPAPEDAERAEQLAELAALEAHEHGDVLQWAFTDTFLNLTLKQVHLLDWLEARCPHARFLLSGDDDVFVHTANVLRFLEAKSPDRHLFTGQLMSGSVPIRESWSKYFVPPQLFPGSVYPVYCSGGGFLLSRHTAQALRQAARHTPLFPIDDAYMGMCLERVGLKPSGHEGIRPFGVQLPGARQPSFDPCMYRELLLVHRFAPYEMLLMWKALHNPGLSCHQGHRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E3K5</accession>
    <name>A0A4W2E3K5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="35 36">Adenylate cyclase type 8</fullName>
            <ecNumber evidence="11 36">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="42" type="primary">ADCY8</name>
    </gene>
    <organism evidence="42 43">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="42 43 44" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="42" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="34">Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion. Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation. Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment. Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells.</text>
    </comment>
    <comment type="function">
        <text evidence="36">Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 36">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <location evidence="8">Caveola</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="33">Membrane</location>
            <location evidence="33">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="31">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="36 38">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027560823.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2E3K5"/>
    <dbReference type="STRING" id="30522.A0A4W2E3K5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020789.1">
        <property type="protein sequence ID" value="ENSBIXP00000033835.1"/>
        <property type="gene ID" value="ENSBIXG00000007751.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056300.1">
        <property type="protein sequence ID" value="ENSBIXP00005046540.1"/>
        <property type="gene ID" value="ENSBIXG00005031309.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904098"/>
    <dbReference type="CTD" id="114"/>
    <dbReference type="GeneTree" id="ENSGT00940000158742"/>
    <dbReference type="OMA" id="HRTIFIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0098686">
        <property type="term" value="C:hippocampal mossy fiber to CA3 synapse"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0014069">
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    </dbReference>
    <dbReference type="GO" id="GO:0048786">
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    </dbReference>
    <dbReference type="GO" id="GO:0042734">
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        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008294">
        <property type="term" value="F:calcium- and calmodulin-responsive adenylate cyclase activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006171">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071377">
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    </dbReference>
    <dbReference type="GO" id="GO:0071315">
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    </dbReference>
    <dbReference type="GO" id="GO:0010255">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150076">
        <property type="term" value="P:neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035774">
        <property type="term" value="P:positive regulation of insulin secretion involved in cellular response to glucose stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900454">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900273">
        <property type="term" value="P:positive regulation of long-term synaptic potentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031915">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080135">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="A/G_cyclase"/>
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        <property type="entry name" value="A/G_cyclase_CS"/>
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        <property type="entry name" value="AC_N"/>
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    <dbReference type="InterPro" id="IPR030672">
        <property type="entry name" value="Adcy"/>
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        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
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        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADENYLATE CYCLASE TYPE 8"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ade_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19 36 37" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="22 36" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="29" id="KW-0966">Cell projection</keyword>
    <keyword evidence="25" id="KW-0168">Coated pit</keyword>
    <keyword evidence="30" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="26" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="28 36 38" id="KW-0456">Lyase</keyword>
    <keyword evidence="20 36" id="KW-0460">Magnesium</keyword>
    <keyword evidence="27" id="KW-0464">Manganese</keyword>
    <keyword evidence="24 36 40" id="KW-0472">Membrane</keyword>
    <keyword evidence="16 36" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0488">Methylation</keyword>
    <keyword evidence="18 36 37" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="43" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0677">Repeat</keyword>
    <keyword evidence="23" id="KW-0770">Synapse</keyword>
    <keyword evidence="15 40" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21 40" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="216"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="246"/>
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    <feature type="transmembrane region" description="Helical" evidence="40">
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            <begin position="296"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="718"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="741"/>
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    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="784"/>
            <end position="808"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="837"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="862"/>
            <end position="882"/>
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    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="41">
        <location>
            <begin position="416"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="41">
        <location>
            <begin position="984"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="39">
        <location>
            <begin position="14"/>
            <end position="33"/>
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    <feature type="region of interest" description="Disordered" evidence="39">
        <location>
            <begin position="51"/>
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    <feature type="region of interest" description="Disordered" evidence="39">
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        <location>
            <begin position="421"/>
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="37">
        <location>
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    <feature type="binding site" evidence="37">
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            <position position="509"/>
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        <ligand>
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    <feature type="binding site" evidence="37">
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        <location>
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        <location>
            <begin position="1118"/>
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    <feature type="binding site" evidence="37">
        <location>
            <position position="1158"/>
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        <ligand>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="30">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00058052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00070495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PIRNR" id="PIRNR039050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="41">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="43">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1253" mass="140031" checksum="BB1682D7CB418A19" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E423</accession>
    <name>A0A4W2E423_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha-tubulin N-acetyltransferase 1</fullName>
            <shortName evidence="7">Alpha-TAT</shortName>
            <shortName evidence="7">Alpha-TAT1</shortName>
            <shortName evidence="7">TAT</shortName>
            <ecNumber evidence="7">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">ATAT1</name>
        <name evidence="7" type="synonym">MEC17</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 7">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <location evidence="7">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell junction</location>
            <location evidence="7">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Autoacetylation strongly increases tubulin acetylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E423"/>
    <dbReference type="STRING" id="30522.A0A4W2E423"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002831.1">
        <property type="protein sequence ID" value="ENSBIXP00000030863.1"/>
        <property type="gene ID" value="ENSBIXG00000012989.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000060">
        <property type="entry name" value="Alpha-tubulin N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038746">
        <property type="entry name" value="Atat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327:SF0">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="7">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine; by autocatalysis" evidence="7">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine; by autocatalysis" evidence="7">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="409" mass="45899" checksum="E7C1DE6035B6B8AA" modified="2019-09-18" version="1">IWLTCPSCFPTVILMEKGVYHPENVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVMYILKDTSARPAGKGAIVGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGRELFQHMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPATSLRATRHSRAAALDPMPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLATDPGGSPAQRRRTSGTPPGLVAQSCSYSRHGRLNSSCPNTGNQELKQGEQETENRCAIEEQVLSQHGSGEEPMHTVPPQAQVPPAQAWTMGGDMFNARFIRNLHERRSTRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E521</accession>
    <name>A0A4W2E521_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113905962</name>
        <name evidence="15" type="synonym">OR51T1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E521"/>
    <dbReference type="STRING" id="30522.A0A4W2E521"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045505.1">
        <property type="protein sequence ID" value="ENSBIXP00000034407.1"/>
        <property type="gene ID" value="ENSBIXG00000017365.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050356.1">
        <property type="protein sequence ID" value="ENSBIXP00005020485.1"/>
        <property type="gene ID" value="ENSBIXG00005023568.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286908"/>
    <dbReference type="OMA" id="TVFYVPM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF6">
        <property type="entry name" value="OLFACTORY RECEPTOR 51T1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="327" mass="37336" checksum="6987A68CBC72AC2D" modified="2019-09-18" version="1">MMIFNNTTSSPSTFLLTAFPGLELAHVWISIPVCCLYIIALLGNTMILFVIFVKQHLHKPMYYFLSMLSAADLCLTITTLPTVLGVLWFHAREISFKACLIQMFFVHAFSFLESSVLAIMAFDRFMAICNPLKYATVLKDMMIMVIGLFICVRQLIFIFPMLLALKTVSFQERKELSHPFCYHPDIIRDTYSNPWINSFLGLFLQLYMTGTDLMFILVSYVLIIRAVLSIVAPKKQKKALNTCVCHICAVTIFYVPMISLSFTHRLLSSTPRVICSILANVYLLLPPVVNPIIYSLKTRTIRQAMLQLLQTKGSWSRNVRGLRGSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7X7</accession>
    <name>A0A4W2E7X7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Probable cystatin-15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC113903442</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027415358.1">
        <property type="nucleotide sequence ID" value="XM_027559557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E7X7"/>
    <dbReference type="STRING" id="30522.A0A4W2E7X7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046303.1">
        <property type="protein sequence ID" value="ENSBIXP00000035518.1"/>
        <property type="gene ID" value="ENSBIXG00000003292.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007124.1">
        <property type="protein sequence ID" value="ENSBIXP00005005066.1"/>
        <property type="gene ID" value="ENSBIXG00005010816.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903442"/>
    <dbReference type="GeneTree" id="ENSGT00940000165454"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052333">
        <property type="entry name" value="Cystatin_spermatogenesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47393:SF1">
        <property type="entry name" value="CYSTATIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47393">
        <property type="entry name" value="CYSTATIN-12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="101"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="211" mass="24021" checksum="83547D538285C773" modified="2019-09-18" version="1">MRGNRACGSLVPKAYLTRGGNWDIPPPHPQLNFPSGDNGRIREEELGRGPGGQQSTVRGRAVLDGELRRRPPKHSASRTTLDNMFWKLPLLLGLLALGPHVCSWMFEDIGKTGQEFSMCVEFAIHHFNEHQPDENAYKLLWVRRSQHKKFSLTYLMDLHLGRTICKKHDEDIDNCPLQEGPEGKKVNCTFIVDSRPLYTKFTLLNSTCQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E824</accession>
    <name>A0A4W2E824_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Growth hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Somatotropin receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GHR</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the type I cytokine receptor family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E824"/>
    <dbReference type="STRING" id="30522.A0A4W2E824"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044460.1">
        <property type="protein sequence ID" value="ENSBIXP00000032955.1"/>
        <property type="gene ID" value="ENSBIXG00000015664.1"/>
    </dbReference>
    <dbReference type="OMA" id="YKPQLYN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000269">
        <property type="entry name" value="Growth hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000318">
        <property type="entry name" value="Growth hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025871">
        <property type="entry name" value="GHBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015152">
        <property type="entry name" value="Growth/epo_recpt_lig-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003528">
        <property type="entry name" value="Long_hematopoietin_rcpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF46">
        <property type="entry name" value="INTERLEUKIN 5 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09067">
        <property type="entry name" value="EpoR_lig-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12772">
        <property type="entry name" value="GHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01352">
        <property type="entry name" value="HEMATOPO_REC_L_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="10 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="22">
        <location>
            <begin position="170"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="657" mass="73432" checksum="16883BD7C2389F12" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EA59</accession>
    <name>A0A4W2EA59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EA59"/>
    <dbReference type="STRING" id="30522.A0A4W2EA59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018653.1">
        <property type="protein sequence ID" value="ENSBIXP00000032922.1"/>
        <property type="gene ID" value="ENSBIXG00000015621.1"/>
    </dbReference>
    <dbReference type="OMA" id="ICVSPHS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000019">
        <property type="entry name" value="C-C motif chemokine 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3 7" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021480096" description="C-C motif chemokine" evidence="7">
        <location>
            <begin position="23"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="9">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="127" mass="14368" checksum="73E3816697123E28" modified="2019-09-18" version="1">MQQTGLALLALAACVAFRPSEAILPIASSCCTEVSHHISRRLLERVTTCRIQRADGDCDLAAVILHVKRRRVCVSPHNHVIKQWMNEQAAKKEAKGNICHKKRHHGRRNSKGAHRERQETHGHKTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDG1</accession>
    <name>A0A4W2EDG1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MGAT4 conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDG1"/>
    <dbReference type="STRING" id="30522.A0A4W2EDG1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005876.1">
        <property type="protein sequence ID" value="ENSBIXP00000029848.1"/>
        <property type="gene ID" value="ENSBIXG00000011588.1"/>
    </dbReference>
    <dbReference type="OMA" id="QPKARYY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF10">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN MGAT4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021253266" description="MGAT4 conserved region domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 conserved region" evidence="3">
        <location>
            <begin position="97"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="376" mass="43903" checksum="E612AA1F57C7F89C" modified="2019-09-18" version="1">MRTRHVNLLITLIAVVLFSFSCFCISRMTQTSNQLTNCRNHISEFKESILRLKNKTERNRQELLNAFKEMKLDISEKERDAQNAVEKKVNASDTNETVSDAFEVLKFFFPHLRKVDRIYPDVIMGREKTGVTFALGISTVNRGNYSYLKQTLTSILSRMTLPEEKDSVVIVSIADSNENYVKSVVDMITKKFKRQVTSGSLEVISIPAYFYPNISHAARSTDDSEKLESWRIKQVLDFCFLMLYAQPKAMYYLQLEDDILANKMYLTKITDFVHNITSNNWLYIEFSVLGFIGKLFKSEDLTDFVRFFLMFYKDKPIDLLLGDIFLVKMCTPGEALEKCIERNKHIRIRYKPSLFQHVGIQSSYPGREQYFKDIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDH4</accession>
    <name>A0A4W2EDH4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDH4"/>
    <dbReference type="SMR" id="A0A4W2EDH4"/>
    <dbReference type="STRING" id="30522.A0A4W2EDH4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023473.1">
        <property type="protein sequence ID" value="ENSBIXP00000034978.1"/>
        <property type="gene ID" value="ENSBIXG00000018103.1"/>
    </dbReference>
    <dbReference type="OMA" id="YQGAMLY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21150">
        <property type="entry name" value="PUA_NSun6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="141"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="242"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="470" mass="51737" checksum="7022F229D6E18E86" modified="2019-09-18" version="1">MSVFPKISLRLEVENYLKAGFMNKEILSALGKQEAERRFEMLLHNLSHPPSFTTVRVNTHLASVQHVKNLLFDELQKQFNGLSFPVLQHPDLQDVLLIPVIGPRKNIKQQTCEVIVGAQCGSAVLRGAHVYVPGIVSASKFMKGGDDVSVYSDIKGKCKKGAKEFDGTKIFLGNGISELSRKEIFSGLPELKGVGVRMTEPVYLSPSFDNVLPSYLFLQNLPSAVVSHVLDPQPGEKILDLCAAPGGKTTHIAALMHDQGEVIALDKISKKVEKIKQNALLLGLNSIRAFCFDGTKALKLDMVKDTDGEPPFLPESFDRILLDAPCSGMGQRPNMACTWTLKEVMSYQPLQRKLFTVAVELLKPGGMLVYSTCTVTLAENEEQVAWALEAFPGLQLQHQDPQVGGEGMLGAGLSPEQLKRLQRFDPSAMRSLDRDIGSLRDASIEDLVWLANKDCIGFFIAKFVKCKSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDJ6</accession>
    <name>A0A4W2EDJ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 47">Phospholipase A2</fullName>
            <ecNumber evidence="6 47">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="50 51">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="50 51" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="50" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="50" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:60657"/>
            <dbReference type="ChEBI" id="CHEBI:74344"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:41076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41123"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72828"/>
            <dbReference type="ChEBI" id="CHEBI:75163"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:41124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 2-O-hexadecyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41271"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:77695"/>
            <dbReference type="ChEBI" id="CHEBI:77696"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41272"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41071"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74344"/>
            <dbReference type="ChEBI" id="CHEBI:77694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:41072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41067"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:55430"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41068"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40427"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73003"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:40428"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + H2O = 1-octadecanoyl-sn-glycero-3-phosphate + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40451"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40452"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40519"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:74965"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:40520"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + glycerol = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + 1-octadecanoyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:41099"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:74965"/>
            <dbReference type="ChEBI" id="CHEBI:75612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41100"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-octadecanoyl-2-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z,15Z)-octadecatrienoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41307"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:78022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:41308"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-octadecanoyl-2-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphocholine + glycerol = 1-(9Z,12Z,15Z-octadecatrienoyl)-glycerol + 1-octadecanoyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:41087"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:75610"/>
            <dbReference type="ChEBI" id="CHEBI:78022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41088"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>2-(prostaglandin E2)-sn-glycero-3-phosphocholine + H2O = prostaglandin E2 + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:137585"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:53693"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>2-(prostaglandin E2)-sn-glycero-3-phosphoethanolamine + H2O = sn-glycero-3-phosphoethanolamine + prostaglandin E2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:137581"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:53705"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>2-[(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoyl]-sn-glycero-3-phosphocholine + H2O = (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:78836"/>
            <dbReference type="ChEBI" id="CHEBI:137582"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:53689"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>2-[(15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl]-sn-glycero-3-phosphocholine + H2O = (15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:78837"/>
            <dbReference type="ChEBI" id="CHEBI:137583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:53697"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>2-[(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl]-sn-glycero-3-phosphocholine + H2O = (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:137584"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:53701"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
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        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:36232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="28">Lipid metabolism; arachidonate metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; leukotriene B4 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; prostaglandin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="29">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="24">Phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="30">Interacts with KAT5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="47">The N-terminal C2 domain associates with lipid membranes upon calcium binding.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="6 47"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDJ6"/>
    <dbReference type="SMR" id="A0A4W2EDJ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029537.1">
        <property type="protein sequence ID" value="ENSBIXP00000037613.1"/>
        <property type="gene ID" value="ENSBIXG00000021210.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005544">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04036">
        <property type="entry name" value="C2_cPLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000030">
        <property type="entry name" value="Phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000002">
        <property type="entry name" value="Phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041847">
        <property type="entry name" value="C2_cPLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040723">
        <property type="entry name" value="cPLA2_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF32">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18695">
        <property type="entry name" value="cPLA2_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16 47" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 47" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="14" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="15 46 47" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="17 46 47" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="18 46 47" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 47" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0595">Phospholipid degradation</keyword>
    <keyword evidence="21" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="51" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="48">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="49">
        <location>
            <begin position="247"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004702"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004716"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022668"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022751"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023408"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00035999"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036797"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00036898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00037916"/>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00037925"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00038814"/>
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    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00047381"/>
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    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00047406"/>
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    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048049"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048087"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048373"/>
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    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00048446"/>
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    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
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    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00048533"/>
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    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00048849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00048903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00049162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00049468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00049471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00049508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="RuleBase" id="RU362102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="48">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="49">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="50">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="51">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="784" mass="88348" checksum="C3705CC9E9EE6A02" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEA2</accession>
    <name>A0A4W2EEA2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Bromodomain and PHD finger containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BRPF3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
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        <property type="entry name" value="Bromodomain-containing protein 1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
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        <property type="entry name" value="Histone_Mod_Regulator"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="BROMODOMAIN AND PHD FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EPL1"/>
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        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BROMODOMAIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_2"/>
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    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 11" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="212"/>
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        <location>
            <begin position="266"/>
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            <begin position="1"/>
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        <location>
            <begin position="387"/>
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        <location>
            <begin position="780"/>
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            <begin position="75"/>
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            <begin position="89"/>
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            <begin position="417"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1207" mass="135900" checksum="0F632401FC327025" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEN5</accession>
    <name>A0A4W2EEN5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pseudouridylate synthase RPUSD2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA pseudouridylate synthase domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Pseudouridine synthase that catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
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            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
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    <dbReference type="SMR" id="A0A4W2EEN5"/>
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        <property type="gene ID" value="ENSBIXG00000021379.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000010">
        <property type="entry name" value="RNA pseudouridine synthase domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="11">
        <location>
            <begin position="238"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="552" mass="61110" checksum="61C8A7814574B11C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEV3</accession>
    <name>A0A4W2EEV3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">NADPH oxidase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">CGD91-phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Cytochrome b(558) subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Cytochrome b-245 heavy chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Heme-binding membrane glycoprotein gp91phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Neutrophil cytochrome b 91 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">gp91-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">gp91-phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">p22 phagocyte B-cytochrome</fullName>
        </alternativeName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. NADPH oxidase complex assembly is impaired through interaction with NRROS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>NADPH + 2 O2 = 2 superoxide + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63180"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="32">Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)). Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2. Interacts with NCF1 (phosphorylated form). Interacts with NCF2; the interaction is enhanced in the presence of GBP7. Interacts with RAC2. Interacts with RAC1. Interacts with calprotectin (S100A8/9). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex. Interacts with CYBC1; CYBC1 may act as a chaperone stabilizing Cytochrome b-245 heterodimer. The CYBA-CYBB complex interacts with GBP7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EEV3"/>
    <dbReference type="STRING" id="30522.A0A4W2EEV3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030057.1">
        <property type="protein sequence ID" value="ENSBIXP00000037822.1"/>
        <property type="gene ID" value="ENSBIXG00000021511.1"/>
    </dbReference>
    <dbReference type="OMA" id="DENQAIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000030">
        <property type="entry name" value="cytochrome b-245 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000004">
        <property type="entry name" value="NADPH oxidase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF12">
        <property type="entry name" value="NADPH OXIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 33" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="34">
        <location>
            <begin position="286"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00042476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00043221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00043223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00050020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00050046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00063468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="566" mass="64924" checksum="D029192E7D365E2A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EH75</accession>
    <name>A0A4W2EH75_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">REEP5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays an essential role in heart function and development by regulating the organization and function of the sarcoplasmic reticulum in cardiomyocytes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408394.1">
        <property type="nucleotide sequence ID" value="XM_027552593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EH75"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015878.1">
        <property type="protein sequence ID" value="ENSBIXP00000031756.1"/>
        <property type="gene ID" value="ENSBIXG00000014156.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029627.1">
        <property type="protein sequence ID" value="ENSBIXP00005038923.1"/>
        <property type="gene ID" value="ENSBIXG00005021148.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899201"/>
    <dbReference type="CTD" id="7905"/>
    <dbReference type="GeneTree" id="ENSGT00940000157873"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF93">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="189" mass="21429" checksum="934779352FCBF7DF" modified="2019-09-18" version="1">MSAAMRQRFDQFLHQKNCMTDLLAKIEAKTGVNRSFIALGVIGLLALYLVFGYGASLLCNLIGFGYPAYVSIKAIESPNKEDDTQWLTYWVVYGVFSIVEFFSDLFLSWFPFYYMLKCGFLLWCMAPSPANGADLLYKRIIRPFFLKHESQVDNVVNDLKDKAKETADTISKEARKAAVSLLGEEKKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EH98</accession>
    <name>A0A4W2EH98_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Importin-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Importin subunit beta-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Karyopherin beta-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ran-binding protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">IPO5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the importin beta family. Importin beta-3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027413656.1">
        <property type="nucleotide sequence ID" value="XM_027557855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EH98"/>
    <dbReference type="SMR" id="A0A4W2EH98"/>
    <dbReference type="STRING" id="30522.A0A4W2EH98"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047386.1">
        <property type="protein sequence ID" value="ENSBIXP00000036987.1"/>
        <property type="gene ID" value="ENSBIXG00000020502.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039660.1">
        <property type="protein sequence ID" value="ENSBIXP00005024442.1"/>
        <property type="gene ID" value="ENSBIXG00005027124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902506"/>
    <dbReference type="CTD" id="3843"/>
    <dbReference type="GeneTree" id="ENSGT00940000155502"/>
    <dbReference type="OMA" id="PKRFVQE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002753">
        <property type="term" value="P:cytoplasmic pattern recognition receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042307">
        <property type="term" value="P:positive regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006610">
        <property type="term" value="P:ribosomal protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000074">
        <property type="entry name" value="importin-5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="15">
        <location>
            <begin position="45"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1097" mass="123581" checksum="A7A17060653B0C2C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIG8</accession>
    <name>A0A4W2EIG8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Matrilin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MATN3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.</text>
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        <text evidence="12">Can form homooligomers (monomers, dimers, trimers and tetramers) and heterooligomers with matrilin-1. Interacts with COMP. Component of a complex containing at least CRELD2, MANF, MATN3 and PDIA4.</text>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
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    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="term" value="P:extracellular matrix organization"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4 14" id="KW-0245">EGF-like domain</keyword>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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        </location>
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            <begin position="47"/>
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    <sequence length="484" mass="52524" checksum="7672711F4EE21CD6" modified="2019-09-18" version="1">MPRHLPGLLLLLWPLLLLPPPPAAPSPMARPGLRRLGTRGPGGSPGRRGPAPAAPASVPYSGTGQPGGARGAGVCKSRPLDLVFIIDSSRSVRPLQFTKVKTFVSKIIDTLDIGPMDTRVAVVNYASTVKIEFHLQTHSDKQSLKRAVARITPLSTGTMSGLAIQTAMDEAFTVEAGARGPSSNIPKVAIIVTDGRPQDQVNEVAARARASGIELYAVGVDRADMESLKMMASEPLDEHVFYVETYGVIEKLSSRFQETFCALDPCALGTHQCQHVCISDGEGRHHCECSQGYSLNADKKTCSAIDKCALNTHGCEHICVNDRTGSYHCECYEGYTLNEDRKTCSAQDQCALGTHGCQHICVNDRAGSHHCECYEGYTLNEDKKTCSVLNQCALGSHGCQHICVYDGNASYHCDCDTGYTLNEDKKTCSAIEEARRLISTEDACACEATLAFQDKVNSYLQRLNSKLDDILEKLQANEYGQVHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJV1</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <accession>A0A4W2EMM2</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="8">Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 9">Belongs to the alpha/beta interferon family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EMM2"/>
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        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="INTERFERON ALPHA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="INTERFERONAB"/>
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        <property type="entry name" value="IFabd"/>
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    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5021317618" evidence="10">
        <location>
            <begin position="22"/>
            <end position="187"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="187" mass="21405" checksum="640E85A2CFF84118" modified="2019-09-18" version="1">MAWSFLLSLLLLSCNTICSLGCLLPHSYSLPNRRVLMLLRQLRRVSPSSCLQDRNDFAFPQEALGSSQLQKAQAISVLHEVTQHTFQLFSTEGSAATWDESLLDKLHAALDQQLTDLQACLRQEEGLRGAPLLKEDSSLAVRKYFHRITLYLREKRHSPCAWEVVRAEVMRAFSSSTNLQERFRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMX7</accession>
    <name>A0A4W2EMX7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMX7"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_4"/>
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        <property type="entry name" value="OLFACTORYR"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
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    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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        <location>
            <begin position="41"/>
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        </location>
    </feature>
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        <source>
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        </source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="35509" checksum="1051B7CBEB1D2964" modified="2019-09-18" version="1">MYGANHSVVLEFVFLGITNSWEIQLLLFVFSSIFFMASIMGNSLIILTVTSDPHLHSPMYFLLANLSFIDMGVSSVSSPKMIYDLFRKHKVISFSGCIAQIFFIHVIGGVEMVLLIAMAFDRYIAICKPLHYLTIMSPRMCVFFLVSAWMIGLMHSMVQLAFVVNLPFCGPNVLDSFYCDLPRFIKLACIDTYQLEFMVTANSGFISVGSFFILIISYIVIILTVQKHSSGGSSKALSTLSAHITVVVLFFGPLIFFYVWPFPSTHLDKFLAFFDAVLTPFLNPVIYTFRNQEMKMAMRRVCQQLYDHIHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EP68</accession>
    <name>A0A4W2EP68_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EP68"/>
    <dbReference type="STRING" id="30522.A0A4W2EP68"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032269.1">
        <property type="protein sequence ID" value="ENSBIXP00000038767.1"/>
        <property type="gene ID" value="ENSBIXG00000022660.1"/>
    </dbReference>
    <dbReference type="OMA" id="KETFATH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="497"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1272" mass="149449" checksum="5A86E64F0B725050" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPA3</accession>
    <name>A0A4W2EPA3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">ADP-ribosylation factor-like protein 2-binding protein</fullName>
            <shortName evidence="13">ARF-like 2-binding protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 13">Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
            <location evidence="1 13">Cytoskeleton</location>
            <location evidence="1 13">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 13">Cytoplasm</location>
            <location evidence="3 13">Cytoskeleton</location>
            <location evidence="3 13">Microtubule organizing center</location>
            <location evidence="3 13">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the ARL2BP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPA3"/>
    <dbReference type="STRING" id="30522.A0A4W2EPA3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032053.1">
        <property type="protein sequence ID" value="ENSBIXP00000038691.1"/>
        <property type="gene ID" value="ENSBIXG00000022569.1"/>
    </dbReference>
    <dbReference type="OMA" id="GYNMAIF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051457">
        <property type="term" value="P:maintenance of protein location in nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1520.10">
        <property type="entry name" value="ADP-ribosylation factor-like 2-binding protein, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038849">
        <property type="entry name" value="ARL2BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042541">
        <property type="entry name" value="BART_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15487">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15487:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 13" id="KW-0969">Cilium</keyword>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="14">
        <location>
            <begin position="37"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="136" mass="15343" checksum="B458798412FA5E40" modified="2019-09-18" version="1">MDIAEVTEVLEEESLAVSTSSTAEAEFDAVVGSREDTIMDYIENQLLERIPGYNMAIFTRTLKHHNDEMVHDVFNMLTCTDFPAFKEMFLEYRTEKEGRRLDLCSGLVVTSLCKSASQNNTKHHLLRPGSGSLLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPL2</accession>
    <name>A0A4W2EPL2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">LIM domain binding 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LDB3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPL2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032354.1">
        <property type="protein sequence ID" value="ENSBIXP00000038814.1"/>
        <property type="gene ID" value="ENSBIXG00000022700.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09454">
        <property type="entry name" value="LIM1_ZASP_Cypher"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09362">
        <property type="entry name" value="LIM2_Enigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09363">
        <property type="entry name" value="LIM3_Enigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000019">
        <property type="entry name" value="LIM domain binding 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000010">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000014">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000020">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF9">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="545"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="604"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="664"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="319"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="468"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="338"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="723" mass="76581" checksum="65EA08DE48D65C33" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EU94</accession>
    <name>A0A4W2EU94_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RNA-binding protein 45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Developmentally-regulated RNA-binding protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RNA-binding motif protein 45</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">RNA-binding protein with binding specificity for poly(C). May play an important role in neural development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EU94"/>
    <dbReference type="STRING" id="30522.A0A4W2EU94"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036394.1">
        <property type="protein sequence ID" value="ENSBIXP00000040575.1"/>
        <property type="gene ID" value="ENSBIXG00000024662.1"/>
    </dbReference>
    <dbReference type="OMA" id="MIPKTYT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12366">
        <property type="entry name" value="RRM1_RBM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12367">
        <property type="entry name" value="RRM2_RBM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12368">
        <property type="entry name" value="RRM3_RBM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12369">
        <property type="entry name" value="RRM4_RBM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000292">
        <property type="entry name" value="RNA-binding motif protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000295">
        <property type="entry name" value="RNA-binding motif protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000290">
        <property type="entry name" value="RNA-binding protein 45 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034203">
        <property type="entry name" value="RBM45_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034206">
        <property type="entry name" value="RBM45_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034207">
        <property type="entry name" value="RBM45_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034208">
        <property type="entry name" value="RBM45_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 17" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="116"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="220"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="489"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="573" mass="63014" checksum="48F23EFD40BDD4D1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVL2</accession>
    <name>A0A4W2EVL2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="9">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EVL2"/>
    <dbReference type="STRING" id="30522.A0A4W2EVL2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037021.1">
        <property type="protein sequence ID" value="ENSBIXP00000040863.1"/>
        <property type="gene ID" value="ENSBIXG00000025024.1"/>
    </dbReference>
    <dbReference type="OMA" id="GCIMYMV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000189">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000600">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF43">
        <property type="entry name" value="INACTIVE SERINE_THREONINE-PROTEIN KINASE PLK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
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    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="29"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="13">
        <location>
            <begin position="392"/>
            <end position="472"/>
        </location>
    </feature>
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        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
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        <location>
            <position position="58"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="548" mass="60677" checksum="7EAC36BFE79DEEDA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVV7</accession>
    <name>A0A4W2EVV7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
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    <dbReference type="STRING" id="30522.A0A4W2EVV7"/>
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        <property type="gene ID" value="ENSBIXG00000005185.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7tmC_mGluR6"/>
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    </dbReference>
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        <property type="entry name" value="PBP1_mGluR_groupIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000009">
        <property type="entry name" value="Glutamate receptor, metabotropic 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000112">
        <property type="entry name" value="GPCR_3__mGluR6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01056">
        <property type="entry name" value="MTABOTROPC6R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021356909" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="15">
        <location>
            <begin position="23"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="615"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="741"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="774"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="805"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="16">
        <location>
            <begin position="577"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="845"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="871" mass="94513" checksum="848E983C3A355378" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXJ6</accession>
    <name>A0A4W2EXJ6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Secretory phospholipase A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">180 kDa secretory phospholipase A2 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">M-type receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Interacts with sPLA2-IB/PLA2G1B; this interaction mediates intracellular signaling as well as clearance of extracellular sPLA2-IB/PLA2G1B via endocytotic pathway. Interacts with sPLA2-X/PLA2G10; this interaction mediates sPLA2-X/PLA2G10 clearance and inactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EXJ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039028.1">
        <property type="protein sequence ID" value="ENSBIXP00000041771.1"/>
        <property type="gene ID" value="ENSBIXG00000025923.1"/>
    </dbReference>
    <dbReference type="OMA" id="GGDICEH"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:receptor-mediated endocytosis"/>
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        <property type="entry name" value="Fibronectin 1a isoform 1"/>
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        <property type="entry name" value="Secretory phospholipase A2 receptor"/>
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        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="C-type_lectin-like"/>
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        <property type="entry name" value="C-type_lectin_CS"/>
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        <property type="entry name" value="Ricin_B-like_lectins"/>
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        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
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        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="FNTYPEII"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="C_TYPE_LECTIN_2"/>
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    </dbReference>
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        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021234723" description="Secretory phospholipase A2 receptor" evidence="21">
        <location>
            <begin position="21"/>
            <end position="1463"/>
        </location>
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        <location>
            <begin position="1398"/>
            <end position="1421"/>
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    </feature>
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        <location>
            <begin position="173"/>
            <end position="221"/>
        </location>
    </feature>
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        <location>
            <begin position="238"/>
            <end position="355"/>
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        <location>
            <begin position="385"/>
            <end position="502"/>
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        <location>
            <begin position="522"/>
            <end position="634"/>
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        <location>
            <begin position="677"/>
            <end position="797"/>
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        <location>
            <begin position="819"/>
            <end position="939"/>
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        <location>
            <begin position="966"/>
            <end position="1097"/>
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    <feature type="domain" description="C-type lectin" evidence="22">
        <location>
            <begin position="1121"/>
            <end position="1224"/>
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        <location>
            <begin position="1257"/>
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            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044135"/>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044268"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044310"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00046689"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1463" mass="168687" checksum="17523CEB56657A38" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYS7</accession>
    <name>A0A4W2EYS7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="21">Calsyntenin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CLSTN2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate synapse formation, and which is involved in learning and memory. Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calsyntenin family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000001514.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Calsyntenin 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calsyntenin 2"/>
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        <property type="entry name" value="Calsyntenin 3"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cadherin-like_dom"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045588">
        <property type="entry name" value="CLSTN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139">
        <property type="entry name" value="CALSYNTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139:SF3">
        <property type="entry name" value="CALSYNTENIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19699">
        <property type="entry name" value="CLSTN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="inferred from homology"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    <evidence type="ECO:0000256" key="25">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042278.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="955" mass="106789" checksum="7DC62A6CB35657C3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZ11</accession>
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        <recommendedName>
            <fullName evidence="14">Sodium/mannose cotransporter SLC5A10</fullName>
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        <alternativeName>
            <fullName evidence="15">Sodium/glucose cotransporter 5</fullName>
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        <alternativeName>
            <fullName evidence="16">Solute carrier family 5 member 10</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="17">Electrogenic Na+-coupled sugar symporter that actively transports D-mannose or D-fructose at the plasma membrane, with a Na+ to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na+ electrochemical gradient set by the Na+/K+ pump. Exclusively recognizes sugar substrates having a pyranose ring with an axial hydroxyl group on carbon 2. Has likely evolved to enable renal reabsorption of D-mannose, an important constituent of oligosaccharide chains of glycoproteins. Contributes to dietary D-fructose reabsorption from glomerular filtrate across the brush border of the kidney.</text>
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        <reaction evidence="13">
            <text>D-fructopyranose(out) + Na(+)(out) = D-fructopyranose(in) + Na(+)(in)</text>
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            <dbReference type="Rhea" id="RHEA:72916"/>
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    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-mannose(out) + Na(+)(out) = D-mannose(in) + Na(+)(in)</text>
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        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:72908"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Na/solute_symporter"/>
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        <property type="entry name" value="Na/solute_symporter_CS"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 4"/>
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    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
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            <end position="55"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
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            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="317"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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    <feature type="transmembrane region" description="Helical" evidence="20">
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        </location>
    </feature>
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            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
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        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036553"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041339"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="681" mass="73813" checksum="68715B2DDFBD06DD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0C5</accession>
    <name>A0A4W2F0C5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="12">Solute carrier family 25 member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphate carrier protein, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Phosphate transport protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Inorganic ion transporter that transports phosphate or copper ions across the mitochondrial inner membrane into the matrix compartment. Mediates proton-coupled symport of phosphate ions necessary for mitochondrial oxidative phosphorylation of ADP to ATP. Transports copper ions probably in the form of anionic copper(I) complexes to maintain mitochondrial matrix copper pool and to supply copper for cytochrome C oxidase complex assembly. May also play a role in regulation of the mitochondrial permeability transition pore (mPTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>phosphate(in) + H(+)(in) = phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:29941"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PPIF; the interaction is impaired by CsA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0C5"/>
    <dbReference type="STRING" id="30522.A0A4W2F0C5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040350.1">
        <property type="protein sequence ID" value="ENSBIXP00000042480.1"/>
        <property type="gene ID" value="ENSBIXG00000005572.1"/>
    </dbReference>
    <dbReference type="OMA" id="PERAYAW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000005">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF8">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="38"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="135"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="236"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="330" mass="36507" checksum="59A85AB28D3A1ACF" modified="2019-09-18" version="1">MCDAGGYQALRGGSAGQLNGRPWAAALEESSYEYGSTKYLLLCGLGGMLSCGLTHTAIVPLDLVKCRMQVDPSKYKGILSGFGVTLRDDGLRGLARGWAPTFLGYSMQGLFKFGLYEVFKIRYAELLGQEKAYEWRTGLYLAASASAEFFADVALAPMEAVKVRMQTQQGYARTLRAAAPKMYGEEGLWAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKYVVPKPQSHCTKSEQLAVTFVAGYIAGVFCAVVSHPADSVVSVLNKEKGSTAFDVLQRLGFKGVWKGLFARIIMIGTLTALQWFIYDSVKVYFKLPRPPVPQAPKSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F203</accession>
    <name>A0A4W2F203_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EF-hand calcium-binding domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EFCAB7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling. Required for the localization of the EVC2:EVC subcomplex at the base of primary cilia.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the EvC complex composed of EFCAB7, IQCE, EVC2 and EVC; built from two subcomplexes, EVC2:EVC and EFCAB7:IQCE. Interacts (via EF-hand 1 and 2) with IQCE (via N-terminus); this interaction anchors the EVC-EVC2 complex in a signaling microdomain at the base of cilia and stimulates the Hedgehog (Hh) pathway. Interacts with EVC2 (via N-terminal end). Interacts with EVC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F203"/>
    <dbReference type="SMR" id="A0A4W2F203"/>
    <dbReference type="STRING" id="30522.A0A4W2F203"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041442.1">
        <property type="protein sequence ID" value="ENSBIXP00000043078.1"/>
        <property type="gene ID" value="ENSBIXG00000027125.1"/>
    </dbReference>
    <dbReference type="OMA" id="KTIDKYW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903569">
        <property type="term" value="P:positive regulation of protein localization to ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000193">
        <property type="entry name" value="EF-hand calcium-binding domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000161">
        <property type="entry name" value="EF-hand calcium-binding domain-containing protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="629" mass="71637" checksum="BEEC6D42D7EA203D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F561</accession>
    <name>A0A4W2F561_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MARCHF11</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F561"/>
    <dbReference type="STRING" id="30522.A0A4W2F561"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020420.1">
        <property type="protein sequence ID" value="ENSBIXP00000011187.1"/>
        <property type="gene ID" value="ENSBIXG00000002557.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054432.1">
        <property type="protein sequence ID" value="ENSBIXP00005000329.1"/>
        <property type="gene ID" value="ENSBIXG00005030353.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160025"/>
    <dbReference type="OMA" id="QWQSITV"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16810">
        <property type="entry name" value="RING_CH-C4HC3_MARCH11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047906">
        <property type="entry name" value="MARCHF11_RING_CH-C4HC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046356">
        <property type="entry name" value="MARCHF4/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="160"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="400" mass="43620" checksum="1152A96DAEAEA90E" modified="2019-09-18" version="1">MSFEGGHSGGRRREAESGDSEPPPSPPPPPPPPPPPGEPASGPAPPRYLPAPPASPERRAGPDEPPAEVVPRHRGAEELPPPPAGQEVAAAGDSGEGPRHLPEATVPEAAAGKGGPGEPEAGAGGEGERRGAGDQPETRSVCSSRSSSSGGGGDHRAGHQHQHHQPICKICFQGAEQGELLNPCRCDGSVRYTHQLCLLKWISERGSWTCELCCYRYHVTAIKMKQPCQWQSISITLVEKVQMIAVILGSLFLIASVTWLLWSAFSPYAVWQRKDILFQICYGMYGFMDLVCIGLIVHEGAAVYRVFKRWRAVNLHWDVLNYDKATDIEESSRGESSTSRTLWLPLTALRNRNLVHPTQLTSPRFQCGYVLLHLFNRMRAHEDLSEDNSSGEVVMRVTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F5E5</accession>
    <name>A0A4W2F5E5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Thioredoxin domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">3'-5' exonuclease NME8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NME/NM23 family member 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Spermatid-specific thioredoxin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. In vitro, it has neither nucleoside diphosphate kinase (NDPK) activity nor reducing activity on disulfide bonds. Exhibits a 3'-5' exonuclease activity with a preference for single-stranded DNA, suggesting roles in DNA proofreading and repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the NDK family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the C-terminal section; belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F5E5"/>
    <dbReference type="STRING" id="30522.A0A4W2F5E5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043406.1">
        <property type="protein sequence ID" value="ENSBIXP00000044225.1"/>
        <property type="gene ID" value="ENSBIXG00000028406.1"/>
    </dbReference>
    <dbReference type="OMA" id="ERQHVSQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04416">
        <property type="entry name" value="NDPk_TX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02948">
        <property type="entry name" value="TRX_NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000012">
        <property type="entry name" value="Thioredoxin domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051766">
        <property type="entry name" value="TXND_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46135">
        <property type="entry name" value="NME/NM23 FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46135:SF2">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="Thioredoxin" evidence="18">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="16">
        <location>
            <position position="559"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="586" mass="67045" checksum="CE4F9B4106F9A08B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F920</accession>
    <name>A0A4W2F920_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Carboxypeptidase B</fullName>
            <ecNumber evidence="17">3.4.17.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CPB1</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Preferential release of a C-terminal lysine or arginine amino acid.</text>
            <dbReference type="EC" id="3.4.17.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Zymogen granule lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.2" evidence="17"/>
    <dbReference type="RefSeq" id="XP_027408261.1">
        <property type="nucleotide sequence ID" value="XM_027552460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F920"/>
    <dbReference type="SMR" id="A0A4W2F920"/>
    <dbReference type="STRING" id="30522.A0A4W2F920"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051106.1">
        <property type="protein sequence ID" value="ENSBIXP00000023705.1"/>
        <property type="gene ID" value="ENSBIXG00000005385.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013423.1">
        <property type="protein sequence ID" value="ENSBIXP00005001842.1"/>
        <property type="gene ID" value="ENSBIXG00005008300.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899126"/>
    <dbReference type="CTD" id="1360"/>
    <dbReference type="GeneTree" id="ENSGT00940000157819"/>
    <dbReference type="OMA" id="TKYRHGT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03871">
        <property type="entry name" value="M14_CPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000002">
        <property type="entry name" value="Carboxypeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034253">
        <property type="entry name" value="CPB_M14_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF20">
        <property type="entry name" value="CARBOXYPEPTIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611295" description="Carboxypeptidase B" evidence="20">
        <location>
            <begin position="16"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="21">
        <location>
            <begin position="118"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="19">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="417" mass="47348" checksum="923DABC54214CB27" modified="2019-09-18" version="1">MLAFLILVTVTLASAHHSGEHFEGDKVFRVHVEDENHISLLHELASTRQMDFWKPDSVTQVKPHSTVDFRVKAEDTVAVEDFLGQNGLRYEVLISNLRSMLEAQFDSRVRTTGHSYEKYNNWETIEAWTEQVASENPDLISRSAIGTTFLGNTIYLLKVGKPGSNKPAVFMDCGFHAREWISPAFCQWFVREAVRTYGREIHMTEFLDKLDFYVLPVVNIDGYIYTWTTNRMWRKTRSTRAGSSCTGTDLNRNFDAGWCSIGASNNPCSETYCGSAAESEKESKAVADFIRNHLSSIKAYLTIHSYSQMMLYPYSYDYKLPKNNVELNTLAKGAVKKLASLHGTTYTYGPGASTIYPASGGSDDWAYDQGIKYSFTFELRDKGRYGFVLPESQIQPTCEETMLAIKYVTSYVLEHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FM15</accession>
    <name>A0A4W2FM15_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MKNK1</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027393173.1">
        <property type="nucleotide sequence ID" value="XM_027537372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FM15"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051066.1">
        <property type="protein sequence ID" value="ENSBIXP00000023614.1"/>
        <property type="gene ID" value="ENSBIXG00000026069.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004424.1">
        <property type="protein sequence ID" value="ENSBIXP00005006420.1"/>
        <property type="gene ID" value="ENSBIXG00005006328.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889937"/>
    <dbReference type="CTD" id="8569"/>
    <dbReference type="GeneTree" id="ENSGT00940000162886"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14174">
        <property type="entry name" value="STKc_Mnk1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000119">
        <property type="entry name" value="Putative map kinase-interacting serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000093">
        <property type="entry name" value="Putative map kinase-interacting serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="37"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="387"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="420" mass="46629" checksum="A981CE444D855AD0" modified="2019-09-18" version="1">MGSSEPIPIAESDKRKKKKRKARATDSLPGKFEDVYKLTSELLGEGANAKVQVAVSLQNGNEYAVKIIEKHAGHSRSRVFREVETLYQCQGNKHILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDEATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCTVCQNKLFESIQKGKYEFPDKDWAHISNEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENEQNKLAEESEALAEGLCSVKLSPPSKSRLARRRALAQAGRSGDAPPSPTPTTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FMP1</accession>
    <name>A0A4W2FMP1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ciliary microtubule inner protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CIMIP7</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FMP1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008392.1">
        <property type="protein sequence ID" value="ENSBIXP00000004165.1"/>
        <property type="gene ID" value="ENSBIXG00000010521.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004372.1">
        <property type="protein sequence ID" value="ENSBIXP00005006447.1"/>
        <property type="gene ID" value="ENSBIXG00005001208.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002177"/>
    <dbReference type="OMA" id="NSGFYGH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029369">
        <property type="entry name" value="HDNR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35539">
        <property type="entry name" value="CDNA SEQUENCE BC048562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35539:SF1">
        <property type="entry name" value="CDNA SEQUENCE BC048562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15115">
        <property type="entry name" value="HDNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function with conserved HDNR motif" evidence="1">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005006447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="205" mass="23591" checksum="C3F0DD62B5332B08" modified="2019-09-18" version="1">MQSALVGSWHNSGFYGHYRGQFRSDSAREYRLAAKPQPPAVFLQRCQEPPQQHFFSKHDNRTSFDKKGPYCLLQGIGRRKDLERLWQQHTFLRWAPCELELRQPRPLESSYQTEFRSGPGLGDLPQRLVHFVQIQPLRVNTTYQQNFCQPSRGGHCGSDNMGPQTPVTNPLPDLPGIPRPKLLQHYLHAGVSECLNWSRTLNKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FSA2</accession>
    <name>A0A4W2FSA2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tissue factor pathway inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TFPI2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2FSA2"/>
    <dbReference type="STRING" id="30522.A0A4W2FSA2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037379.1">
        <property type="protein sequence ID" value="ENSBIXP00000022311.1"/>
        <property type="gene ID" value="ENSBIXG00000025216.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000485.1">
        <property type="protein sequence ID" value="ENSBIXP00005008370.1"/>
        <property type="gene ID" value="ENSBIXG00005013434.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159917"/>
    <dbReference type="OMA" id="REEYFFN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22615">
        <property type="entry name" value="Kunitz_TFPI1_TFPI2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22616">
        <property type="entry name" value="Kunitz_TFPI2_1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000004">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000011">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000018">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008296">
        <property type="entry name" value="TFPI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001620">
        <property type="entry name" value="TFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="3 13" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044535897" description="Tissue factor pathway inhibitor" evidence="13">
        <location>
            <begin position="23"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="36"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="96"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="240" mass="27327" checksum="32E29714E1AA2957" modified="2019-09-18" version="1">MDSVRPLWLMLLSLLLVGTALGDASQAPPGNNAEICLLPPDDGPCRARIPSYYYDRYTQSCREFMYGGCEGNANNFETLEACNEACWKIEKVPKICRLKVNKKQCGELREQYFFNLSSMTCKKFISGGCHSNENRFPDEATCMDFCAPKRGKYSLCAPVFCYSPKDEGLCSANVTRYYFNPRHKACEAFNYTGCGGNDNNFVNLKDCKRTCVKALKKEKNKKMPRLLLANRRLKIKKKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GAE8</accession>
    <name>A0A4W2GAE8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ARFRP1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SYS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414983.1">
        <property type="nucleotide sequence ID" value="XM_027559182.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027414985.1">
        <property type="nucleotide sequence ID" value="XM_027559184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GAE8"/>
    <dbReference type="SMR" id="A0A4W2GAE8"/>
    <dbReference type="STRING" id="30522.A0A4W2GAE8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027428.1">
        <property type="protein sequence ID" value="ENSBIXP00000015760.1"/>
        <property type="gene ID" value="ENSBIXG00000000451.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025367.1">
        <property type="protein sequence ID" value="ENSBIXP00005014842.1"/>
        <property type="gene ID" value="ENSBIXG00005018785.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903283"/>
    <dbReference type="CTD" id="10139"/>
    <dbReference type="GeneTree" id="ENSGT00940000156407"/>
    <dbReference type="OMA" id="HGFYKYM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04160">
        <property type="entry name" value="Arfrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000509">
        <property type="entry name" value="ADP-ribosylation factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005014842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="201" mass="22644" checksum="F1CBFD139A73520A" modified="2019-09-18" version="1">MYTLLSGLYKYMFQKDEYCVLILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTVGLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLSESKQAFEKMVTSEALDGVPILVLANKQDVETCLSIPDIKTAFSDCASKIGRRDCLTQACSALTGKGVREGIEWMVKCVVRNVHRPPRQRDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GCB1</accession>
    <name>A0A4W2GCB1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase delta subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA polymerase delta subunit p50</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">POLD2</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GCB1"/>
    <dbReference type="STRING" id="30522.A0A4W2GCB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026871.1">
        <property type="protein sequence ID" value="ENSBIXP00000015428.1"/>
        <property type="gene ID" value="ENSBIXG00000019946.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026458.1">
        <property type="protein sequence ID" value="ENSBIXP00005015534.1"/>
        <property type="gene ID" value="ENSBIXG00005019352.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006780"/>
    <dbReference type="OMA" id="TLIWRHY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000001">
        <property type="entry name" value="DNA polymerase delta 2, accessory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000008">
        <property type="entry name" value="DNA polymerase delta 2, accessory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.430:FF:000001">
        <property type="entry name" value="DNA polymerase delta subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="13">
        <location>
            <begin position="146"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="12">
        <location>
            <begin position="293"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005015534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="580" mass="62588" checksum="E72E228BD49917B9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GE59</accession>
    <name>A0A4W2GE59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Flattop</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cilia- and flagella-associated protein 126</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CFAP126</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme. Acts as a regulator of cilium basal body docking and positioning in mono- and multiciliated cells. Regulates basal body docking and cilia formation in multiciliated lung cells. Regulates kinocilium positioning and stereocilia bundle morphogenesis in the inner ear.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Flattop family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GE59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051928.1">
        <property type="protein sequence ID" value="ENSBIXP00000024992.1"/>
        <property type="gene ID" value="ENSBIXG00000027124.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027501.1">
        <property type="protein sequence ID" value="ENSBIXP00005016239.1"/>
        <property type="gene ID" value="ENSBIXG00005003269.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001092"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23705">
        <property type="entry name" value="Flattop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038797">
        <property type="entry name" value="Fltp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34639">
        <property type="entry name" value="PROTEIN FLATTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34639:SF1">
        <property type="entry name" value="PROTEIN FLATTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22611">
        <property type="entry name" value="CFAP126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="177" mass="19288" checksum="EC553B48CA24F349" modified="2019-09-18" version="1">MATNYSANQYEKPFSPKYLQNWSLAKPTKERISSHEGYTQIIANDRGHLLPSVPRSKASPWGSFMGTWQMPLKVPPARATLTSRTAAGAASLTRWIQKNPDLLKASNGLRPEIFGKPHDPDSQKKLRKSITKTVQQAPSPTIIPSSPASNLSSPDQLQSSHPSAGHTPGPQSPLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GEX9</accession>
    <name>A0A4W2GEX9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PHD finger protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PHF11</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027413086.1">
        <property type="nucleotide sequence ID" value="XM_027557285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GEX9"/>
    <dbReference type="SMR" id="A0A4W2GEX9"/>
    <dbReference type="STRING" id="30522.A0A4W2GEX9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013175.1">
        <property type="protein sequence ID" value="ENSBIXP00000027487.1"/>
        <property type="gene ID" value="ENSBIXG00000008353.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028416.1">
        <property type="protein sequence ID" value="ENSBIXP00005016877.1"/>
        <property type="gene ID" value="ENSBIXG00005020432.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902243"/>
    <dbReference type="CTD" id="51131"/>
    <dbReference type="GeneTree" id="ENSGT00950000182865"/>
    <dbReference type="OMA" id="ECEDHDS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000425">
        <property type="entry name" value="PHD finger protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF4">
        <property type="entry name" value="PHD FINGER PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="42"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="337" mass="37951" checksum="54B9B4CED498F68D" modified="2019-09-18" version="1">MDTAGPSAPESTRGASWSEAQAARDIVPLPTGVFQVAERLQKRICALCPKDLECSVLYFAQSENIAAHENCLLYSSALVECEDYDPSNNDRNFDVESVKKEIKRGRKLKCTFCGKKGATVGCDLKSCFKNYHFFCAKNDHAVLQADGRTGIYKVFCQQHADPQNDLPSVKPLSGVFHSHYSEQTKPRHACFSGVKRKRGRSKRHHVQPPERMALKKEKDGRHTDAIVKAAFLKKCKEAGLLDALFEEILDKLHLIQERLMDETTAESDYEEIGTSLFDCRLFEDTLVNFQAAIENQIHQSEERRRQLKEEIELLQDLKQTLCSGLQSSSTSDSSLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GFP8</accession>
    <name>A0A4W2GFP8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">F-box only protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">FBXO11</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the SCF(FBXO11) complex consisting of CUL1, RBX1, SKP1 and FBXO11. Interacts with CIITA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GFP8"/>
    <dbReference type="STRING" id="30522.A0A4W2GFP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022127.1">
        <property type="protein sequence ID" value="ENSBIXP00000012291.1"/>
        <property type="gene ID" value="ENSBIXG00000017375.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029501.1">
        <property type="protein sequence ID" value="ENSBIXP00005017571.1"/>
        <property type="gene ID" value="ENSBIXG00005021079.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063425"/>
    <dbReference type="OMA" id="KESFHQL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010719">
        <property type="term" value="P:negative regulation of epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22091">
        <property type="entry name" value="F-box_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19676">
        <property type="entry name" value="UBR-box_UBR6_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000003">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000005">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000006">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000007">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="3"/>
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    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
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    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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        <property type="entry name" value="F-box_dom"/>
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    <dbReference type="InterPro" id="IPR047505">
        <property type="entry name" value="F-box_FBXO11"/>
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    <dbReference type="InterPro" id="IPR047504">
        <property type="entry name" value="FBXO11_UBR-box"/>
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        <property type="entry name" value="Para_beta_helix_rpt-2"/>
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        <property type="entry name" value="PbH1"/>
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    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
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    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
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        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
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        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
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        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF20">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-box" evidence="15">
        <location>
            <begin position="146"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="UBR-type" evidence="16">
        <location>
            <begin position="826"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="13">
        <location>
            <begin position="826"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063474"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068830"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="920" mass="102794" checksum="1D602A6B98962B55" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GGN9</accession>
    <name>A0A4W2GGN9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mth938 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AAMDC</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role in preadipocyte differentiation and adipogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAMDC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388498.1">
        <property type="nucleotide sequence ID" value="XM_027532697.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388499.1">
        <property type="nucleotide sequence ID" value="XM_027532698.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388501.1">
        <property type="nucleotide sequence ID" value="XM_027532700.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388502.1">
        <property type="nucleotide sequence ID" value="XM_027532701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GGN9"/>
    <dbReference type="SMR" id="A0A4W2GGN9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013161.1">
        <property type="protein sequence ID" value="ENSBIXP00000001816.1"/>
        <property type="gene ID" value="ENSBIXG00000008364.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028869.1">
        <property type="protein sequence ID" value="ENSBIXP00005017177.1"/>
        <property type="gene ID" value="ENSBIXG00005020691.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886481"/>
    <dbReference type="CTD" id="28971"/>
    <dbReference type="GeneTree" id="ENSGT00390000011958"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045600">
        <property type="term" value="P:positive regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05126">
        <property type="entry name" value="Mth938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1230.10:FF:000001">
        <property type="entry name" value="Adipogenesis-associated, Mth938 domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAMDC"/>
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    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15811">
        <property type="entry name" value="MTH938 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15811:SF5">
        <property type="entry name" value="MTH938 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00057418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005017177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="122" mass="13388" checksum="B68842036A088221" modified="2019-09-18" version="1">MSSPEIASLSWGQMKVQGSTKIYKDCKVWPGGSRDWDWRETGTEHSPGVQPADVEEVVEKGVQILVIGRGMSEALKVPPSTVEYLKKKGIDVRVLQTEQAVKEYNALATQGIRVGGVFHSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GSG7</accession>
    <name>A0A4W2GSG7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metalloproteinase inhibitor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Tissue inhibitor of metalloproteinases 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TIMP3</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with EFEMP1. Interacts with KDR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GSG7"/>
    <dbReference type="SMR" id="A0A4W2GSG7"/>
    <dbReference type="STRING" id="30522.A0A4W2GSG7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051936.1">
        <property type="protein sequence ID" value="ENSBIXP00000025001.1"/>
        <property type="gene ID" value="ENSBIXG00000005693.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034264.1">
        <property type="protein sequence ID" value="ENSBIXP00005020773.1"/>
        <property type="gene ID" value="ENSBIXG00005004074.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159601"/>
    <dbReference type="OMA" id="PFGKCET"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010716">
        <property type="term" value="P:negative regulation of extracellular matrix disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03585">
        <property type="entry name" value="NTR_TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.370.10:FF:000001">
        <property type="entry name" value="Metalloproteinase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000005">
        <property type="entry name" value="Metalloproteinase inhibitor 3 precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030490">
        <property type="entry name" value="TIMP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF22">
        <property type="entry name" value="METALLOPROTEINASE INHIBITOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00288">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="6" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="NTR" evidence="18">
        <location>
            <begin position="131"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="44"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="131"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="133"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="143"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="252"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="257"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030101"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00063810"/>
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    </evidence>
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            <dbReference type="PIRSR" id="PIRSR601820-1"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020773.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="318" mass="35233" checksum="C196C9B1CE43CAC7" modified="2019-09-18" version="1">MIFQHAFGLSPPFCLKSACNHWLLGLEKHSSSCSSARRDCPPAQSCASAEAAPSHPIPSRRALGERRAPSQSCPPQAGGRAQAAATRPVARKATLETRAAAPATAAAMTPWLGLVVLLGSWSLGDWGAEACTCSPSHPQDAFCNSDIVIRAKVVGKKLLKEGPFGTMVYTIKQMKMYRGFTKMPHVQYIHTEASESLCGLKLEVNKYQYLLTGRVYDGKMYTGLCNFVERWDQLTLSQRKGLNYRYHLGCNCKIKSCYYLPCFVTSKNECLWTDMLSNFGYPGYQSKHYACIRQKGGYCSWYRGWAPPDKSIINATDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GU13</accession>
    <name>A0A4W2GU13_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GU13"/>
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        <property type="protein sequence ID" value="ENSBIXP00000039186.1"/>
        <property type="gene ID" value="ENSBIXG00000023128.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034338.1">
        <property type="protein sequence ID" value="ENSBIXP00005020820.1"/>
        <property type="gene ID" value="ENSBIXG00005023831.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="20" mass="2201" checksum="99766C3193E86314" modified="2019-09-18" version="1">NNNNNLRFGAGTRLTVKPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HCK1</accession>
    <name>A0A4W2HCK1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Poly(U)-binding-splicing factor PUF60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">60 kDa poly(U)-binding-splicing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PUF60</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM half pint family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416298.1">
        <property type="nucleotide sequence ID" value="XM_027560497.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2HCK1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003182.1">
        <property type="protein sequence ID" value="ENSBIXP00000030741.1"/>
        <property type="gene ID" value="ENSBIXG00000006569.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015528.1">
        <property type="protein sequence ID" value="ENSBIXP00005027917.1"/>
        <property type="gene ID" value="ENSBIXG00005029853.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903887"/>
    <dbReference type="CTD" id="22827"/>
    <dbReference type="GeneTree" id="ENSGT00940000155594"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12370">
        <property type="entry name" value="RRM1_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12371">
        <property type="entry name" value="RRM2_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12648">
        <property type="entry name" value="RRM3_UHM_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000133">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000136">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000152">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006532">
        <property type="entry name" value="PUF60-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051974">
        <property type="entry name" value="PUF60_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034209">
        <property type="entry name" value="PUF60_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034211">
        <property type="entry name" value="PUF60_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034212">
        <property type="entry name" value="PUF60_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01645">
        <property type="entry name" value="half-pint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330:SF1">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="100"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="197"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="433"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="530" mass="57043" checksum="BB9C5E066E7C8F46" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HD21</accession>
    <name>A0A4W2HD21_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RPS2</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Interacts with zinc finger protein ZNF277 (via zinc-finger domains); the interaction is direct; the interaction is extra-ribosomal. Interaction with ZNF277 competes with the binding of RPS2 to protein arginine methyltransferase PRMT3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HD21"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015398.1">
        <property type="protein sequence ID" value="ENSBIXP00000026816.1"/>
        <property type="gene ID" value="ENSBIXG00000029344.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054323.1">
        <property type="protein sequence ID" value="ENSBIXP00005027670.1"/>
        <property type="gene ID" value="ENSBIXG00005030271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153095"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000133">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="72"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="209" mass="21576" checksum="EC83CD18B706865C" modified="2019-09-18" version="1">MADDAGAAGGPGGPGGPGMGGRGGFRGGFGSGVRGRGRGRGRGRGRGRGARGGKAEDKEESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFGHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HHC1</accession>
    <name>A0A4W2HHC1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ETS translocation variant 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ets-related protein 71</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ETV2</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HHC1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019163.1">
        <property type="protein sequence ID" value="ENSBIXP00000010378.1"/>
        <property type="gene ID" value="ENSBIXG00000015865.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011487.1">
        <property type="protein sequence ID" value="ENSBIXP00005029211.1"/>
        <property type="gene ID" value="ENSBIXG00005009145.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162407"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000340">
        <property type="entry name" value="ETS translocation variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF209">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="11">
        <location>
            <begin position="52"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17856" checksum="0CAB38670997E328" modified="2019-09-18" version="1">MDLWNWDEVSPQEVPLGHRMSGLEGAELNFYIPELAVQADTLTEDTCWKGPIQLWQFLLELLHDGARRSCIRWTGNSREFQLCDPKEVARLWGERKRKPGMNYEKLSRGLRYYYRRDIVRKSGGRKYTYRFGGRLPSLVYPDLMGGGLEAPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HKI7</accession>
    <name>A0A4W2HKI7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Proton-coupled zinc antiporter SLC30A9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 30 member 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Zinc transporter 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">SLC30A9</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Mitochondrial proton-coupled zinc ion antiporter mediating the export of zinc from the mitochondria and involved in zinc homeostasis, zinc mobilization as well as mitochondrial morphology and health. In nucleus, functions as a secondary coactivator for nuclear receptors by cooperating with p160 coactivators subtypes. Plays a role in transcriptional activation of Wnt-responsive genes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Zn(2+)(in) + 2 H(+)(out) = Zn(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027400187.1">
        <property type="nucleotide sequence ID" value="XM_027544386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HKI7"/>
    <dbReference type="SMR" id="A0A4W2HKI7"/>
    <dbReference type="STRING" id="30522.A0A4W2HKI7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038474.1">
        <property type="protein sequence ID" value="ENSBIXP00000023059.1"/>
        <property type="gene ID" value="ENSBIXG00000025648.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005004287.1">
        <property type="protein sequence ID" value="ENSBIXP00005031590.1"/>
        <property type="gene ID" value="ENSBIXG00005006425.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894241"/>
    <dbReference type="CTD" id="10463"/>
    <dbReference type="GeneTree" id="ENSGT00390000008346"/>
    <dbReference type="OMA" id="HSMFSEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21078">
        <property type="entry name" value="NTD_ZNT9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000004">
        <property type="entry name" value="zinc transporter 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.530.10:FF:000002">
        <property type="entry name" value="zinc transporter 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040177">
        <property type="entry name" value="SLC30A9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414">
        <property type="entry name" value="HUEL-CATION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414:SF9">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="24">
        <location>
            <begin position="238"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00059884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="566" mass="63165" checksum="38AE489925B2FD5D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HQH6</accession>
    <name>A0A4W2HQH6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-hydroxytryptamine receptor 1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 1A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">HTR1A</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Heterodimer; heterodimerizes with GPER1. Interacts with YIF1B. Interacts with GPR39 and GALR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376212.1">
        <property type="nucleotide sequence ID" value="XM_027520411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HQH6"/>
    <dbReference type="SMR" id="A0A4W2HQH6"/>
    <dbReference type="STRING" id="30522.A0A4W2HQH6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044019.1">
        <property type="protein sequence ID" value="ENSBIXP00000032396.1"/>
        <property type="gene ID" value="ENSBIXG00000001864.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017047.1">
        <property type="protein sequence ID" value="ENSBIXP00005033388.1"/>
        <property type="gene ID" value="ENSBIXG00005014129.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879142"/>
    <dbReference type="CTD" id="3350"/>
    <dbReference type="GeneTree" id="ENSGT00940000163614"/>
    <dbReference type="OMA" id="VQHCNSS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15330">
        <property type="entry name" value="7tmA_5-HT1A_vertebrates"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000610">
        <property type="entry name" value="5HT1A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF191">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00512">
        <property type="entry name" value="5HT1ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0085">Behavior</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="53"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="237"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005033388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="422" mass="46445" checksum="E542F791EAA92ED2" modified="2019-09-18" version="1">MDVLSPGQGNNTTSSQGPFGTRGNATGISDVTFSYQVITSLVLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAITDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVDKKAANHRLAASPAPQPRKSVSGESDSRDWRQGTENKVTGAPCANGAVRQGEEGAALEVIEVHRVGNSKEHLPLPSEGGAVPYVPASFEKKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HSU3</accession>
    <name>A0A4W2HSU3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CD48 antigen-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC113884642</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027385215.1">
        <property type="nucleotide sequence ID" value="XM_027529414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HSU3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040574.1">
        <property type="protein sequence ID" value="ENSBIXP00000042613.1"/>
        <property type="gene ID" value="ENSBIXG00000026589.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019072.1">
        <property type="protein sequence ID" value="ENSBIXP00005034296.1"/>
        <property type="gene ID" value="ENSBIXG00005006557.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884642"/>
    <dbReference type="GeneTree" id="ENSGT01030000234540"/>
    <dbReference type="OMA" id="EKGNCHT"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015631">
        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080:SF121">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611951" evidence="6">
        <location>
            <begin position="34"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="152"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="365" mass="41073" checksum="7CA854331942F89D" modified="2019-09-18" version="1">MDSLGSSWLPTQTLILQISLIFGLSSPSPDSQAVKYPSSSCTLKRTVGTSVQLTLTSPLHPNVREIEWNWKSEDDEKTRLLVSWKPQTPNPDWYELEEIYKHRLNLTEMAFLSIRNLTMEMSGLYTAKIKFNSGKSNEEEFRLCLYDPIPHPQIQILSSSNTSGWCNISLECEIPGNAGNLTVTWLSQGLPRHLKQRGILPNSRNLSLSLPISHINSHLTCVVSNPAEEKNATLHLEDICPWRGSLQSKWLWLGILLMVLMVSLGAGVWIWMRKKMQTRRGGSTLLPEASGLKATPQALPTEESTNLQTDVTNSLDSPYEEISFQRHLKKDLEKGNCHTDNPECTLAIHTVYEKIRTSPEPQGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HVR4</accession>
    <name>A0A4W2HVR4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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            <end position="364"/>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF6">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN-CONTAINING 11-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12125">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01028">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="150"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="257"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="380"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="420"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="460"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="509"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005037566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="563" mass="64539" checksum="35F41CE06A2BDF47" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I5Y2</accession>
    <name>A0A4W2I5Y2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pleckstrin homology domain containing A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PLEKHA1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027385380.1">
        <property type="nucleotide sequence ID" value="XM_027529579.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027385381.1">
        <property type="nucleotide sequence ID" value="XM_027529580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I5Y2"/>
    <dbReference type="SMR" id="A0A4W2I5Y2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002931.1">
        <property type="protein sequence ID" value="ENSBIXP00000006873.1"/>
        <property type="gene ID" value="ENSBIXG00000012941.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030315.1">
        <property type="protein sequence ID" value="ENSBIXP00005039204.1"/>
        <property type="gene ID" value="ENSBIXG00005021579.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884706"/>
    <dbReference type="CTD" id="59338"/>
    <dbReference type="GeneTree" id="ENSGT00940000155157"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13270">
        <property type="entry name" value="PH1_TAPP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13271">
        <property type="entry name" value="PH2_TAPP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000049">
        <property type="entry name" value="pleckstrin homology domain-containing family A member 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000042">
        <property type="entry name" value="pleckstrin homology domain-containing family A member 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="7"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="191"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="383" mass="43632" checksum="23B2F7EBAABE8256" modified="2019-09-18" version="1">MPYVDRQNRICGFLDIEENENSGKFLRRYFILDTREDSFVWYMDNPQNLPSGSSRVGAIKLTYISKVSDATKLRPKAEFCFVMNAGMRKYFLQANDQQDLVEWVNVLNKAIKITVPKPLDSQPHPDNPSRQGECGKKQVSYRTDIVGGVPIITPTQKEEVNECGEAIDRNNLKRSQSHLPYFTPKPPSDSAVIKAGYCVKQGAVMKNWKRRYFQLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTSSRTFYVQADSPEEMHSWIKAVSGAIVAQRGPGRSASSMRQARRLSNPCIQRSIPTVLPSPNTLSSLPVPQQPPHIPQPLAATLWSQAFPWRSEDFMNLLPRSSQGTSRSRLSLQENQLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I8L3</accession>
    <name>A0A4W2I8L3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]</fullName>
            <ecNumber evidence="3">3.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Nucleoside diphosphate-linked moiety X motif 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NUDT2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the asymmetric hydrolysis of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to yield AMP and ATP. Exhibits decapping activity towards FAD-capped RNAs and dpCoA-capped RNAs in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GMP + GTP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57553"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5'-end CoA-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + (R)-4'-phosphopantetheine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67592"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="Rhea" id="RHEA-COMP:17276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
            <dbReference type="ChEBI" id="CHEBI:172371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67593"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end FAD-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + FMN + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67588"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="Rhea" id="RHEA-COMP:17275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
            <dbReference type="ChEBI" id="CHEBI:172372"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67589"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027405850.1">
        <property type="nucleotide sequence ID" value="XM_027550049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I8L3"/>
    <dbReference type="SMR" id="A0A4W2I8L3"/>
    <dbReference type="STRING" id="30522.A0A4W2I8L3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036319.1">
        <property type="protein sequence ID" value="ENSBIXP00000040541.1"/>
        <property type="gene ID" value="ENSBIXG00000024623.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049072.1">
        <property type="protein sequence ID" value="ENSBIXP00005039195.1"/>
        <property type="gene ID" value="ENSBIXG00005007216.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897370"/>
    <dbReference type="CTD" id="318"/>
    <dbReference type="GeneTree" id="ENSGT00390000002416"/>
    <dbReference type="OMA" id="WRDYEQA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006167">
        <property type="term" value="P:AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
        <property type="term" value="P:ATP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03428">
        <property type="entry name" value="NUDIX_Ap4A_Nudt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000037">
        <property type="entry name" value="Nudix hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003565">
        <property type="entry name" value="Tetra_PHTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01405">
        <property type="entry name" value="TETRPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="14">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="147" mass="16775" checksum="1BC4523DF285D130" modified="2019-09-18" version="1">MALRACGLIIFRRRLIPKVDNTAIEFLLLQASDGIHHWTPPKGHVEPGESDLETALRETQEEAGIEAGQLTIIEGFRRELSYVARAKPKIVIYWLAEVKDCDVEVRLSREHQAYRWLELEDACQLAQFEEMKAALQEGHQFLCSTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IKF1</accession>
    <name>A0A4W2IKF1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 22">Glutamine synthetase</fullName>
            <ecNumber evidence="22">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">GLUL</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Decamer; composed of two pentamers. Interacts with PALMD. Interacts with RHOJ. Interacts with BEST2; this interaction tethers a fraction of GLUL to the membrane, causing a decrease of cytosolic glutamine synthase (GS) activity and inhibits the chloride channel activity of BEST2 by affecting the gating at the aperture in the absence of intracellular glutamate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 20 21">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="22"/>
    <dbReference type="RefSeq" id="XP_027421310.1">
        <property type="nucleotide sequence ID" value="XM_027565509.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027421311.1">
        <property type="nucleotide sequence ID" value="XM_027565510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IKF1"/>
    <dbReference type="SMR" id="A0A4W2IKF1"/>
    <dbReference type="STRING" id="30522.A0A4W2IKF1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016450.1">
        <property type="protein sequence ID" value="ENSBIXP00000008676.1"/>
        <property type="gene ID" value="ENSBIXG00000014453.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053446.1">
        <property type="protein sequence ID" value="ENSBIXP00005044650.1"/>
        <property type="gene ID" value="ENSBIXG00005028507.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906836"/>
    <dbReference type="CTD" id="2752"/>
    <dbReference type="GeneTree" id="ENSGT00390000010047"/>
    <dbReference type="OMA" id="DRRPNAN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF45">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="14 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 22" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="23">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="24">
        <location>
            <begin position="113"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005044650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="373" mass="42031" checksum="BBCC6A953FD7DAD5" modified="2019-09-18" version="1">MATSASSHLNKGIKQVYMALPQGDKVQAMYIWIDGTGEGLRCKTRTLDSEPKCIEELPEWNFDGSSTFQSEGSNSDMYLVPAAMFRDPFRKDPNKLVFCEVFKYNRKPAETNLRHTCKRIMDMVSNQRPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADKAYGRDIVEAHYRACLYAGIKIGGTNAEVMPAQWEFQIGPCEGIDMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRGASIRIPRTVGQEKKGYFEDRRPSANCDPFAVTEALIRTCLLNETGDEPFQYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BN90</accession>
    <name>A0A4W2BN90_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase MYLIP</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inducible degrader of the LDL-receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase MYLIP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">MYLIP</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with the E2 ubiquitin-conjugating enzyme, UBE2D1 (via RING-type zinc finger). Interacts with myosin regulatory light chain (MRLC) and TMEM4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027380076.1">
        <property type="nucleotide sequence ID" value="XM_027524275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BN90"/>
    <dbReference type="STRING" id="30522.A0A4W2BN90"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014035.1">
        <property type="protein sequence ID" value="ENSBIXP00000001437.1"/>
        <property type="gene ID" value="ENSBIXG00000028563.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043036.1">
        <property type="protein sequence ID" value="ENSBIXP00005045090.1"/>
        <property type="gene ID" value="ENSBIXG00005005057.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881322"/>
    <dbReference type="CTD" id="29116"/>
    <dbReference type="GeneTree" id="ENSGT00940000156206"/>
    <dbReference type="OMA" id="NKGENLW"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010989">
        <property type="term" value="P:negative regulation of low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010977">
        <property type="term" value="P:negative regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032803">
        <property type="term" value="P:regulation of low-density lipoprotein particle receptor catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13195">
        <property type="entry name" value="FERM_C_MYLIP_IDOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17104">
        <property type="entry name" value="FERM_F1_MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16523">
        <property type="entry name" value="RING-HC_MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000019">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000129">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYLIP isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000164">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000175">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041790">
        <property type="entry name" value="MYLIP_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FERM" evidence="18">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="387"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="49559" checksum="8BE38023FD1342C1" modified="2019-09-18" version="1">MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKEALLAGHLQCSPEQAVELSALLAQTKFGDYNQNTAKYSYEELCAKELSSATLNSIVAKHKELEGTSQASAEYQVLQIVSAMENYGIEWHSVRDSEGQKLLIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEAYDHARRALYNAGVVDLLPRSDPSPPNSPLKSSESSTSCSSCEGLGCQQTRALQEKLRKLKEAMLCVLCCEGEINSAFCPCGHTVCCEGCAAQLQSCPVCRSRVDHIQHVYLPTHTSLLNLTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNA1</accession>
    <name>A0A4W2BNA1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FLYWCH family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FLYWCH2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027382302.1">
        <property type="nucleotide sequence ID" value="XM_027526501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNA1"/>
    <dbReference type="STRING" id="30522.A0A4W2BNA1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053117.1">
        <property type="protein sequence ID" value="ENSBIXP00000000455.1"/>
        <property type="gene ID" value="ENSBIXG00000029814.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013470.1">
        <property type="protein sequence ID" value="ENSBIXP00005028576.1"/>
        <property type="gene ID" value="ENSBIXG00005008285.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883139"/>
    <dbReference type="CTD" id="114984"/>
    <dbReference type="GeneTree" id="ENSGT00530000064166"/>
    <dbReference type="OMA" id="PTVCPSM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029279">
        <property type="entry name" value="FLYWCH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040312">
        <property type="entry name" value="FWCH1/FWCH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31665:SF0">
        <property type="entry name" value="FLYWCH FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31665">
        <property type="entry name" value="FLYWCH FAMILY MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15423">
        <property type="entry name" value="FLYWCH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FLYWCH-type zinc finger-containing protein N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="144" mass="14950" checksum="37FF77B97C68B374" modified="2019-09-18" version="1">MPLPEPSEQEGESVKAGQEPSPEPPQPGTDVVPAAPKKPRKFSKLVLLTASKDSAKVEGAKRKGVHCIMSLGVPGPATLAKALLKIHPEAQRAIEAAPQEPEQKRSKLDTDGEEDGSLAGPSAPSPLVDGEESTSALVKPRAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRN7</accession>
    <name>A0A4W2BRN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbonic anhydrase 4</fullName>
            <ecNumber evidence="5">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Carbonate dehydratase IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Carbonic anhydrase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CA4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:10749"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:10750"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with SLC4A4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027374540.1">
        <property type="nucleotide sequence ID" value="XM_027518739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BRN7"/>
    <dbReference type="SMR" id="A0A4W2BRN7"/>
    <dbReference type="STRING" id="30522.A0A4W2BRN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011369.1">
        <property type="protein sequence ID" value="ENSBIXP00000002704.1"/>
        <property type="gene ID" value="ENSBIXG00000000239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034072.1">
        <property type="protein sequence ID" value="ENSBIXP00005020641.1"/>
        <property type="gene ID" value="ENSBIXG00005023689.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877985"/>
    <dbReference type="CTD" id="762"/>
    <dbReference type="GeneTree" id="ENSGT00940000155690"/>
    <dbReference type="OMA" id="AVEFHLH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03117">
        <property type="entry name" value="alpha_CA_IV_XV_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041874">
        <property type="entry name" value="CA4/CA15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF95">
        <property type="entry name" value="CARBONIC ANHYDRASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610272" description="Carbonic anhydrase 4" evidence="21">
        <location>
            <begin position="20"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="312" mass="35151" checksum="BAEA320C09426351" modified="2019-09-18" version="1">MRLLLALLVLAAAPPQARAASHWCYQIQVKPSNYTCLEPDEWEGSCQNNRQSPVNIVTAKTQLDPNLGRFSFSGYNMKHQWVVQNNGHTVMVLLENKPSIAGGGLSTRYQATQLHLHWSRAMDRGSEHSFDGERFAMEMHIVHEKEKGLSGNASQNQFAEDEIAVLAFMVEDGSKNVNFQPLVEALSDIPRPNMNTTMKEGVSLFDLLPEEESLRHYFRYLGSLTTPTCDEKVVWTVFQKPIQLHRDQILAFSQKLFYDDQQKVNMTDNVRPVQSLGQRQVFRSGAPGLLLAQPLPTLLAPVLACLTVGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRZ2</accession>
    <name>A0A4W2BRZ2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="445"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BS91</accession>
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        <recommendedName>
            <fullName evidence="15">Coiled-coil domain-containing protein 88B</fullName>
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        <alternativeName>
            <fullName evidence="17">Hook-related protein 3</fullName>
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        <name evidence="22" type="primary">CCDC88B</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="14">Homodimer. Interacts with DOCK8. Interacts (via C-terminus) with intact microtubules. Interacts with dynein-dynactin motor complex. Interacts (via C-terminus) with HSPA5.</text>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
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        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="13">Belongs to the CCDC88 family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000002927.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 88B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hook domain"/>
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    </dbReference>
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    <keyword evidence="20" id="KW-0732">Signal</keyword>
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    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021189614" description="Coiled-coil domain-containing protein 88B" evidence="20">
        <location>
            <begin position="26"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="domain" description="HOOK N-terminal" evidence="21">
        <location>
            <begin position="50"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="504"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="565"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1311"/>
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        </location>
    </feature>
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        <location>
            <begin position="253"/>
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        </location>
    </feature>
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        <location>
            <begin position="454"/>
            <end position="481"/>
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    </feature>
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        <location>
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        <location>
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            <end position="1029"/>
        </location>
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        <location>
            <begin position="1072"/>
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    </feature>
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        <location>
            <begin position="625"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
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        </location>
    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00061299"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064574"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00068574"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00078746"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF19047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1463" mass="163302" checksum="EF9CA3713C0B8A59" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTW7</accession>
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        <recommendedName>
            <fullName evidence="2">Immediate early response 3-interacting protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="12" type="primary">IER3IP1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="9">Regulator of endoplasmic reticulum secretion that acts as a key determinant of brain size. Required for secretion of extracellular matrix proteins. Required for correct brain development by depositing sufficient extracellular matrix proteins for tissue integrity and the proliferation of neural progenitors. Acts as a regulator of the unfolded protein response (UPR).</text>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Yos1"/>
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    <dbReference type="PANTHER" id="PTHR15858">
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        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="Yos1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="142" mass="15615" checksum="C48C1303DA608DB5" modified="2019-09-18" version="1">SHFQAAALDSAGLHSSADTPCRGERRAGLSQTGRSLSGRVLGLASCRRKRRTWRRHVPCRRGRGVSWALGWVAMAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVRTVMRGKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVN4</accession>
    <name>A0A4W2BVN4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVN4"/>
    <dbReference type="SMR" id="A0A4W2BVN4"/>
    <dbReference type="STRING" id="30522.A0A4W2BVN4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010118.1">
        <property type="protein sequence ID" value="ENSBIXP00000003308.1"/>
        <property type="gene ID" value="ENSBIXG00000009720.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23701">
        <property type="entry name" value="At1g26750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059242">
        <property type="entry name" value="mS23_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR019520">
        <property type="entry name" value="Ribosomal_mS23_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15925">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15925:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10484">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
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        <location>
            <begin position="2"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="190" mass="21578" checksum="EDD491AE7F9348CB" modified="2019-09-18" version="1">MAGSRLETVGSIFTRTRDLIRAGVLKEKPLWFDVYNAFPPLREPVFRRPRLRYGKAKSPTQDIFYHEDQIRAKFYAAYGSGPKAFDLFNPNFKSTCQRFVEKYIELQKLGETDEEKLFVEAGKALLAEGVILRRVEKARTQQEGSQVSRKSESMGVESQTALEENPPLKEVPQAQHLESPGEESKGLSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWG2</accession>
    <name>A0A4W2BWG2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
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        <property type="gene ID" value="ENSBIXG00000010014.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="458" mass="52056" checksum="E653393A737037E8" modified="2019-09-18" version="1">MPAHLENSAVAQDWKRSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANICWIIEKARELQKNIYFCFIDYVKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQACILSPCLFNLYAEYIMRNAGLEEAEAGIKIAGRNINNLRYADDTTLMAESEEELKSLLKKVKVESEKVGVKLNIQKTKIMVSGPIISWEIDGEAVETVSDFIFGGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAIVFPVVMYGCESWTVKKAECQRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQCFGHLMGRVDSLEKTVMLGGIGGRRRRGRQRMRWLDGITNSMDVSLGELRELVMDREAWSAAVHGVAKSWTRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BX67</accession>
    <name>A0A4W2BX67_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">chymotrypsin</fullName>
            <ecNumber evidence="9">3.4.21.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.1" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BX67"/>
    <dbReference type="SMR" id="A0A4W2BX67"/>
    <dbReference type="STRING" id="30522.A0A4W2BX67"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008980.1">
        <property type="protein sequence ID" value="ENSBIXP00000003881.1"/>
        <property type="gene ID" value="ENSBIXG00000010266.1"/>
    </dbReference>
    <dbReference type="OMA" id="WGFRIAD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000118">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000176">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250">
        <property type="entry name" value="CHYMOTRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250:SF65">
        <property type="entry name" value="CHYMOTRYPSINOGEN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021442424" description="chymotrypsin" evidence="11">
        <location>
            <begin position="19"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="34"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="263" mass="27604" checksum="7F5EE1ADDDD23CC6" modified="2019-09-18" version="1">MAPLWLLSCFALVGAAFGCGVPAIQPVLSGLARIVNGEDAVPGSWPWQVSLQDSTGFHFCGGSLISEDWVVTAAHCGVTTSDVVVAGEFDQGLQTEDTQVLKIGKVFKNPKFSILTVRNDITLLKLATPAQFSETVSAVCLPSADEDFPAGMLCATTGWGKTKYNALKTPDKLQQATLPIVSNTDCRKYWGSRVTDVMICAGASGVSSCMGDSGGPLVCQKNGAWTLAGIVSWGSSTCSTSTPAVYARVTALMPWVQETLAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C0D9</accession>
    <name>A0A4W2C0D9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AarF domain containing kinase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ADCK5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027416274.1">
        <property type="nucleotide sequence ID" value="XM_027560473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C0D9"/>
    <dbReference type="SMR" id="A0A4W2C0D9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004304.1">
        <property type="protein sequence ID" value="ENSBIXP00000006171.1"/>
        <property type="gene ID" value="ENSBIXG00000012302.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903881"/>
    <dbReference type="CTD" id="203054"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF28">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="170"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="580" mass="66057" checksum="0702AECECF8FF122" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C4J0</accession>
    <name>A0A4W2C4J0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">StAR-related lipid transfer protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">START domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">STARD5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in the intracellular transport of sterols or other lipids. May bind cholesterol or other sterols.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377337.1">
        <property type="nucleotide sequence ID" value="XM_027521536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C4J0"/>
    <dbReference type="SMR" id="A0A4W2C4J0"/>
    <dbReference type="STRING" id="30522.A0A4W2C4J0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001354.1">
        <property type="protein sequence ID" value="ENSBIXP00000007667.1"/>
        <property type="gene ID" value="ENSBIXG00000013606.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018208.1">
        <property type="protein sequence ID" value="ENSBIXP00005010003.1"/>
        <property type="gene ID" value="ENSBIXG00005014761.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879765"/>
    <dbReference type="CTD" id="80765"/>
    <dbReference type="GeneTree" id="ENSGT00940000159159"/>
    <dbReference type="OMA" id="PQKVWEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070508">
        <property type="term" value="P:cholesterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000031">
        <property type="entry name" value="StAR-related lipid transfer protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043556">
        <property type="entry name" value="StARD5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46374">
        <property type="entry name" value="PROTEIN CBG07384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46374:SF3">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="7">
        <location>
            <begin position="11"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="213" mass="23573" checksum="3183BDC2E38D2946" modified="2019-09-18" version="1">MDLATAAQVSEAVAEKMLQYRRDKSGWKICREGNGVSVSWRPSVEFPGNLYKGEGIVNGTPEQVWDCVKPLAGTLRAQWDENVNSFEIIESLTDTLLFSRTTTPSAVMKLISPRDFVDLILVRTYEDGTISSNAANVEHPSCPPNPAYVRGFNHPCGCFCEPLPGEPNKTSLVTFFQTDLSGYLPQSVVDSFFPRSMAGFYANLEKAVKKFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6E4</accession>
    <name>A0A4W2C6E4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BANP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">BANP</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Part of a corepressor complex containing BANP, HDAC1, SIN3A, SIN3B, RBL1 and RBL2. Forms a trimeric complex in the nucleus consisting of BANP, HDAC6 and KHDRBS1/SAM68; HDAC6 keeps KHDRBS1 in a deacetylated state which inhibits the inclusion of CD44 alternate exons. The complex is disrupted by MAPK1/MAPK3-mediated phosphorylation of BANP which results in BANP export to the cytoplasm. This facilitates acetylation of KHDRBS1 and CD44 variant exon inclusion. Interacts with TP53. Interacts with CUX1/CDP. Interacts with HDAC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BANP/SMAR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6E4"/>
    <dbReference type="STRING" id="30522.A0A4W2C6E4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015998.1">
        <property type="protein sequence ID" value="ENSBIXP00000008352.1"/>
        <property type="gene ID" value="ENSBIXG00000014217.1"/>
    </dbReference>
    <dbReference type="OMA" id="TQQVQIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042177">
        <property type="term" value="P:negative regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2590:FF:000001">
        <property type="entry name" value="protein BANP isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042343">
        <property type="entry name" value="BANP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16243">
        <property type="entry name" value="BTG3-ASSOCIATED NUCLEAR PROTEIN BANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16243:SF2">
        <property type="entry name" value="PROTEIN BANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BEN"/>
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    </dbReference>
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        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="343"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="444"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009735"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015794"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="647" mass="69395" checksum="00964F79B768DC88" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6P4</accession>
    <name>A0A4W2C6P4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase RNF10</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING finger protein 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling. Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome. Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation. The action of RNF10 in iRQC is counteracted by USP10. May also act as a transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression. Acts as a regulator of Schwann cell differentiation and myelination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with MEOX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNF10 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C6P4"/>
    <dbReference type="STRING" id="30522.A0A4W2C6P4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016140.1">
        <property type="protein sequence ID" value="ENSBIXP00000008463.1"/>
        <property type="gene ID" value="ENSBIXG00000014301.1"/>
    </dbReference>
    <dbReference type="OMA" id="PRWKKCP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031643">
        <property type="term" value="P:positive regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16536">
        <property type="entry name" value="RING-HC_RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000112">
        <property type="entry name" value="RING finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="225"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="603"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="727"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="780"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="612"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="667"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="727"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="815" mass="90259" checksum="B7020121F8B31362" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C743</accession>
    <name>A0A4W2C743_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C743"/>
    <dbReference type="STRING" id="30522.A0A4W2C743"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001936.1">
        <property type="protein sequence ID" value="ENSBIXP00000007398.1"/>
        <property type="gene ID" value="ENSBIXG00000013357.1"/>
    </dbReference>
    <dbReference type="OMA" id="SCDHSFA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001851">
        <property type="term" value="F:complement component C3b binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000026">
        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000054">
        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000060">
        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF7">
        <property type="entry name" value="COMPLEMENT FACTOR H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021324942" description="Sushi domain-containing protein" evidence="9">
        <location>
            <begin position="28"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="94"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="156"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="158"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="278" mass="31921" checksum="CA49058B3985BDF3" modified="2019-09-18" version="1">MNLPLRMSNMLLLLSVILTLWVCCAHGRGKTCDFPEIKHGSIYNENRYKKIFPADVGKYFYYTCEHSFVTPSQSLWTRINCTEEGWSPTPKCLRQCFFPWVENGQSTSSGQTHREGDIVHIVCDTGYSLPNGQSNITCGERGWSSPPECRHVYPRGRCGPPPPIDNGDTVSFPLSQYPPGSAVEYQCQAYYVLQGNRHIVCRNGEWSEPPKCLDACVISEEMMKQHNIQLKWRDDKKLYTRTDDTIEFTCKRGYRPTTPIRTFRTTCHEGKVVYPHCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8T6</accession>
    <name>A0A4W2C8T6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C8T6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000682.1">
        <property type="protein sequence ID" value="ENSBIXP00000007988.1"/>
        <property type="gene ID" value="ENSBIXG00000013848.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018926.1">
        <property type="protein sequence ID" value="ENSBIXP00005010520.1"/>
        <property type="gene ID" value="ENSBIXG00005015107.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282489"/>
    <dbReference type="OMA" id="DNSHEIK"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="26"/>
            <end position="289"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="481" mass="55325" checksum="FFABA694096A54D4" modified="2019-09-18" version="1">MEFQLNSRIQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVLIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYMNRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIHFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYSGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNFYAEYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSGFIFGGSKITADGDCSHEIKRRLLLGRKVMTNPDSIFKSRDITLPTKVHLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWSARRSNQSILKEISPGISLEGMMLRLKLQYFGHLMRRADSLAKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVTDREAWRAAIPGVSKSRTQLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9E4</accession>
    <name>A0A4W2C9E4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Parathymosin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PTMS</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pro/parathymosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396967.1">
        <property type="nucleotide sequence ID" value="XM_027541166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C9E4"/>
    <dbReference type="STRING" id="30522.A0A4W2C9E4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015724.1">
        <property type="protein sequence ID" value="ENSBIXP00000008200.1"/>
        <property type="gene ID" value="ENSBIXG00000014080.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047853.1">
        <property type="protein sequence ID" value="ENSBIXP00005037652.1"/>
        <property type="gene ID" value="ENSBIXG00005019569.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892368"/>
    <dbReference type="CTD" id="5763"/>
    <dbReference type="GeneTree" id="ENSGT01030000234816"/>
    <dbReference type="OMA" id="HEEKSAH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004931">
        <property type="entry name" value="Pro/parathymosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22745:SF3">
        <property type="entry name" value="PARATHYMOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22745">
        <property type="entry name" value="PROTHYMOSIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03247">
        <property type="entry name" value="Prothymosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="102" mass="11486" checksum="E0F2DFD50A63ED56" modified="2019-09-18" version="1">MSEKSVEAAAELSAKDLKEKKEKVEEKAGRKERKKEVVEEEENGAEEEEEETAEDGEEEDEGDEEDEEEEEDEDEGPALKRAAEEEDEADPKRQKTENGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CDD5</accession>
    <name>A0A4W2CDD5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SOGA coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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        </alternativeName>
    </protein>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
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            <text>Preferential cleavage where P1', P2' and P3' are hydrophobic residues.</text>
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        </reaction>
    </comment>
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            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
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    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="24">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
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            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.17" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CH59"/>
    <dbReference type="STRING" id="30522.A0A4W2CH59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005334.1">
        <property type="protein sequence ID" value="ENSBIXP00000005637.1"/>
        <property type="gene ID" value="ENSBIXG00000011802.1"/>
    </dbReference>
    <dbReference type="OMA" id="NFVQQYL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF215">
        <property type="entry name" value="STROMELYSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0177">Collagen degradation</keyword>
    <keyword evidence="17 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 29" id="KW-0732">Signal</keyword>
    <keyword evidence="11 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021189530" description="Stromelysin-1" evidence="29">
        <location>
            <begin position="18"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="30">
        <location>
            <begin position="105"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="287"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="337"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="385"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="434"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="27">
        <location>
            <begin position="90"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="22">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="24">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="124"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="158"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="168"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="170"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="175"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
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        <location>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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    <feature type="binding site" evidence="24">
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
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            <begin position="290"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="28">
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="477" mass="54387" checksum="96CFE5619A0B2744" modified="2019-09-18" version="1">MRNLPILLLLCVAVCSAYPLDRAARDKEDTMELVQQYLENYYNLAKDTKQFVRRKDSSPVVQKIQEMQKFLGLEVTGKLDSDTLEVIRKPRCGIPDVGFFSTFPGSPKWRKTHLTYRIVNYTKDLPRDAVDSAIEKALTVWEEVTPLTFSRIYEGEADIMIIFAVREHGDFLPFDGPGKVLAHAYPPGSGFYGDAHFDDDEQWTKDTSGINLFLVAAHELGHSLGLQHSTEREALMYPVYDPRTDLTRFRLSQDDVDGIQFLYGSPPVSPNDPVVPTESVPPEPGTPAACDPALSFDAVSTLRGEILFFKGRHFWRKSFRTFEPEFHLISSFWPSLPSGIDAAYEVTSKDTVFIFKGNQFWAIRGNEMQAGYPRGIHTLGFPSTVRKIDAAFSDKEKKKTYFFAEDKYWRFDEKRQSMEPGFPKQIVEDFPGVESEVDAVFEVFGFYYFFSGSSQFEFDPNAKKVTHVLKSNSWLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHG1</accession>
    <name>A0A4W2CHG1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHG1"/>
    <dbReference type="SMR" id="A0A4W2CHG1"/>
    <dbReference type="STRING" id="30522.A0A4W2CHG1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005149.1">
        <property type="protein sequence ID" value="ENSBIXP00000005738.1"/>
        <property type="gene ID" value="ENSBIXG00000011882.1"/>
    </dbReference>
    <dbReference type="OMA" id="LYTADPF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072711">
        <property type="term" value="P:cellular response to hydroxyurea"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097752">
        <property type="term" value="P:regulation of DNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01796">
        <property type="entry name" value="Ubl_Ddi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000005">
        <property type="entry name" value="DNA damage inducible 1 homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000107">
        <property type="entry name" value="protein DDI1 homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057273">
        <property type="entry name" value="Ddi1/2_HDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033882">
        <property type="entry name" value="DDI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24669">
        <property type="entry name" value="Ddi2_HDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="367"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="396" mass="43921" checksum="A2E9241029752726" modified="2019-09-18" version="1">MLLTVYCVRRDLSEATFSLQVRPDFELHNFRVLCELESGIPAEETQIVYMERLLVNDHCSLGSYGLKDGDMVILLQKEAMRPRSPERAAGLCSMEPAGPALPGTSGSRPHQRAQSAQHSSRRGSGEKAGPGQGLDSPALVRSMLLSSPHDLSLLKERNPSLAEALLSGNLESFSQVLMEQQRERALREQERLRLFSADPFDLEAQAKIEEEIRQQNIEENMSIAMEEAPESFGQVAMLYINCRVNGHPLKAFVDSGAQMTIMNQVCAERCNIIRLVDRRWAGVAKGVGTQRILGRVHLAQIQIEGDFLQCSFSILEEQPMDMLLGLDMLRRHQCSIDLKRNVLVIGTTGTQTSFLPEGELPPCAKLVSGMGPEESSDKETANAIKHSVMDSGRKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI29</accession>
    <name>A0A4W2CI29_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger protein 541</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ZNF541</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CI29"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022823.1">
        <property type="protein sequence ID" value="ENSBIXP00000012743.1"/>
        <property type="gene ID" value="ENSBIXG00000017797.1"/>
    </dbReference>
    <dbReference type="OMA" id="FAYDCPD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004357">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000251">
        <property type="entry name" value="Zinc finger protein 541"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000656">
        <property type="entry name" value="Zinc finger protein 541"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051066">
        <property type="entry name" value="Trans_reg/Corepressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089">
        <property type="entry name" value="REST COREPRESSOR COREST PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 541"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="827"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="ELM2" evidence="19">
        <location>
            <begin position="1045"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="20">
        <location>
            <begin position="1152"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="1283"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="418"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="558"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="678"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="875"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1209"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="916"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1209"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1258"/>
            <end position="1267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1344" mass="145543" checksum="463FE93966E457A0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIP3</accession>
    <name>A0A4W2CIP3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">E3 ubiquitin-protein ligase RNF169</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">RING finger protein 169</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase RNF169</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Probable E3 ubiquitin-protein ligase that acts as a regulator of double-strand breaks (DSBs) repair following DNA damage. Functions in a non-canonical fashion to harness RNF168-mediated protein recruitment to DSB-containing chromatin, thereby contributing to regulation of DSB repair pathway utilization. Once recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168, competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby favouring homologous recombination repair (HRR) and single-strand annealing (SSA) instead of non-homologous end joining (NHEJ) mediated by TP53BP1. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Interacts with DYRK1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the RNF169 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CIP3"/>
    <dbReference type="STRING" id="30522.A0A4W2CIP3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021097.1">
        <property type="protein sequence ID" value="ENSBIXP00000011618.1"/>
        <property type="gene ID" value="ENSBIXG00000016855.1"/>
    </dbReference>
    <dbReference type="OMA" id="EFIFRAP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21951">
        <property type="entry name" value="MIU_RNF169_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16551">
        <property type="entry name" value="RING-HC_RNF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22264">
        <property type="entry name" value="UDM1_RNF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000676">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051657">
        <property type="entry name" value="RNF168/RNF169_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="26">
        <location>
            <begin position="68"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="117"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="197"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="287"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="508"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="197"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00067427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="708" mass="77356" checksum="CB4D78CBB942790E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CK62</accession>
    <name>A0A4W2CK62_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CK62"/>
    <dbReference type="STRING" id="30522.A0A4W2CK62"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003255.1">
        <property type="protein sequence ID" value="ENSBIXP00000006696.1"/>
        <property type="gene ID" value="ENSBIXG00000001214.1"/>
    </dbReference>
    <dbReference type="OMA" id="VHLDLTW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF35">
        <property type="entry name" value="PROTON CHANNEL OTOP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="563" mass="62083" checksum="6B0646B05D5D6204" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLD3</accession>
    <name>A0A4W2CLD3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL16m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLD3"/>
    <dbReference type="SMR" id="A0A4W2CLD3"/>
    <dbReference type="STRING" id="30522.A0A4W2CLD3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022865.1">
        <property type="protein sequence ID" value="ENSBIXP00000012759.1"/>
        <property type="gene ID" value="ENSBIXG00000007186.1"/>
    </dbReference>
    <dbReference type="OMA" id="WGHMEMM"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000005">
        <property type="entry name" value="39S ribosomal protein L16, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="251" mass="28573" checksum="9C03E0163DF55229" modified="2019-09-18" version="1">MWRLLSGARAPVLRATLSDSWAAPPARAGLKTLLPVPTFEDVSIPEKPKLRFVERAPLVPKVRREPKNLRDIRGPSTEATEFTEGNFAILALGGGYLHWGHFEMMRLTINRFMDPKNMFALWRVPAPFKAITRKGMGQRMGGGKGAIDHYVTPVKAGRLIVEVGGRCEFQEVQGILNQVAHKLPFPAKAVSRETLEKIQKDQEERERNNQNPWTFERIATANMLGIRKVLSPYDLTHRGRYWGKFYMPERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMC3</accession>
    <name>A0A4W2CMC3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium/calcium exchanger 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Na(+)/Ca(2+)-exchange protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 8 member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">SLC8A1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline. Required for normal embryonic heart development and the onset of heart contractions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMC3"/>
    <dbReference type="STRING" id="30522.A0A4W2CMC3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023104.1">
        <property type="protein sequence ID" value="ENSBIXP00000012924.1"/>
        <property type="gene ID" value="ENSBIXG00000017971.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050393.1">
        <property type="protein sequence ID" value="ENSBIXP00005020562.1"/>
        <property type="gene ID" value="ENSBIXG00005024054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155129"/>
    <dbReference type="OMA" id="TFSMGCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030506">
        <property type="term" value="F:ankyrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030501">
        <property type="term" value="P:positive regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098735">
        <property type="term" value="P:positive regulation of the force of heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035994">
        <property type="term" value="P:response to muscle stretch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000003">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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</entry>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="787"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="708"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="802"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="911" mass="98407" checksum="B880A6CE09112B54" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQE4</accession>
    <name>A0A4W2CQE4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thioredoxin reductase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TXNRD2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQE4"/>
    <dbReference type="STRING" id="30522.A0A4W2CQE4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024967.1">
        <property type="protein sequence ID" value="ENSBIXP00000014182.1"/>
        <property type="gene ID" value="ENSBIXG00000018949.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000012">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF7">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="138"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="636" mass="67695" checksum="215C493E91190643" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSD1</accession>
    <name>A0A4W2CSD1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidylprolyl isomerase domain and WD repeat-containing protein 1</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PPWD1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. May be involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Identified in the spliceosome C complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSD1"/>
    <dbReference type="SMR" id="A0A4W2CSD1"/>
    <dbReference type="STRING" id="30522.A0A4W2CSD1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017267.1">
        <property type="protein sequence ID" value="ENSBIXP00000009175.1"/>
        <property type="gene ID" value="ENSBIXG00000014883.1"/>
    </dbReference>
    <dbReference type="OMA" id="GMVEYWR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000394">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000119">
        <property type="entry name" value="peptidylprolyl isomerase domain and WD repeat-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="4 17" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="127"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="19">
        <location>
            <begin position="488"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="644" mass="73274" checksum="9EDCC2AEB77BAB18" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWH2</accession>
    <name>A0A4W2CWH2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cilia and flagella associated protein 95</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CFAP95</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CWH2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047391.1">
        <property type="protein sequence ID" value="ENSBIXP00000016274.1"/>
        <property type="gene ID" value="ENSBIXG00000020509.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020064.1">
        <property type="protein sequence ID" value="ENSBIXP00005011256.1"/>
        <property type="gene ID" value="ENSBIXG00005015744.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014593"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027905">
        <property type="entry name" value="CFAP95"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35069:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35069">
        <property type="entry name" value="PROTEIN C9ORF135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15139">
        <property type="entry name" value="CFAP95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="232" mass="26637" checksum="E3B13A054AE426F7" modified="2019-09-18" version="1">MDSSDSSCQEWCSHGQYWLELGPTDLMERKGSLTLRSHHKKYSKPVLVYSWHRDRETYPKDYDIEGPEEVKKLCNSTYRRLGTSEPPVWISETREKMAQVCLNTKLAKIKSKALLNEETMNSGIIERDTGLPATGFGALFTRHSPDWSKMCTLTTYAEEYAPPYEYQPLGDPCQDDDYSIVHRKCRSQFTDLDGSKRLGINTWHDESGIYANSYVKRKLYSLTGGPIAPFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXG2</accession>
    <name>A0A4W2CXG2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">sphingomyelin phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SMPD2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-O-octadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-octadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74001"/>
            <dbReference type="ChEBI" id="CHEBI:75216"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:39924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a sphingosylphosphocholine + H2O = a sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:84410"/>
            <dbReference type="ChEBI" id="CHEBI:85171"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:45297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N-(acyl)-sphingosylphosphocholine + H2O = an N-acyl-sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:45301"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXG2"/>
    <dbReference type="STRING" id="30522.A0A4W2CXG2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020161.1">
        <property type="protein sequence ID" value="ENSBIXP00000011024.1"/>
        <property type="gene ID" value="ENSBIXG00000016399.1"/>
    </dbReference>
    <dbReference type="OMA" id="LWTPNVG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="20">
        <location>
            <begin position="203"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="17"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="614" mass="67478" checksum="E4E98153257D5E1C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYG0</accession>
    <name>A0A4W2CYG0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor CP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TFCP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398684.1">
        <property type="nucleotide sequence ID" value="XM_027542883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYG0"/>
    <dbReference type="SMR" id="A0A4W2CYG0"/>
    <dbReference type="STRING" id="30522.A0A4W2CYG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031545.1">
        <property type="protein sequence ID" value="ENSBIXP00000018480.1"/>
        <property type="gene ID" value="ENSBIXG00000022317.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023000.1">
        <property type="protein sequence ID" value="ENSBIXP00005013243.1"/>
        <property type="gene ID" value="ENSBIXG00005017401.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893140"/>
    <dbReference type="CTD" id="7024"/>
    <dbReference type="GeneTree" id="ENSGT00940000157629"/>
    <dbReference type="OMA" id="XNLLPTT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042789">
        <property type="term" value="P:mRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09589">
        <property type="entry name" value="SAM_TFCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000022">
        <property type="entry name" value="Transcription factor CP2 like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037599">
        <property type="entry name" value="TFCP2_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF11">
        <property type="entry name" value="ALPHA-GLOBIN TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="9">
        <location>
            <begin position="61"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="501" mass="57127" checksum="FD279176B776576B" modified="2019-09-18" version="1">MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIFRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGIIDPRANPNQLNTVEFLWDPAKRTSVFIQVHCISTEFTMRKHGGEKGVPFRVQIDTFKENENGEYTEHLHSASCQIKVFKPKGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPWPEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRLFTNFSGADLLKLTRDDVIQICGPADGIRLFNALKGRMVRPRLTIYVCQESLQLREQQQQQQNQQKHEDGDSNGTFFVYHAIYLEELTAVELTEKIAQLFSISPCQISQIYKQGPTGIHVLISDEMIQNFQEEACFILDTMKAETNDSYHIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1A2</accession>
    <name>A0A4W2D1A2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1A2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049025.1">
        <property type="protein sequence ID" value="ENSBIXP00000019606.1"/>
        <property type="gene ID" value="ENSBIXG00000023198.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="482" mass="54047" checksum="BD8E9DACC5ABB9CC" modified="2019-09-18" version="1">MDELVHDLASALEQTSEQNKLGELWEEMALSPRQQRRQLRKRRGRKRRSDFTHLAEHTCCYSEASESSLDEALKDCREMAPVTNFSDSDDTMVVKRHPALNAIVKSKQHSWHESDSFTENAPCRPLRRRRKVKRVTSEVAASLQQKLKVSDWSYERGCRFKSAKKQRLSRWKENTPWTSSGHGLCESAENRTFLSKTGRKERMECEADEQKQGSDENMSECETSSVCSSSDTGLFTNDEGRQGDDEQSDWFYEGECVPGFTVPNLLPKWAPDHCSEVERMDSGLDKLSDSTFLLPSRPAQRGYHARFNRLPGAAARCLRKGRRRLVGKESSISNLGTERIGHLISDPRQKEKNKALASGFPHISACAHEFNPLSPLYSLDVLADASHRRCSPAHCSARQANIHLGPPCSRDIKRKRKPVATVSLSSPNAVHMDAVESATAASQAPRPPDSEWLVRTSAAEKATDSAAATSFKMPQEKSPGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1H6</accession>
    <name>A0A4W2D1H6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ethanolamine kinase</fullName>
            <ecNumber evidence="5">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1H6"/>
    <dbReference type="STRING" id="30522.A0A4W2D1H6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022450.1">
        <property type="protein sequence ID" value="ENSBIXP00000012498.1"/>
        <property type="gene ID" value="ENSBIXG00000017567.1"/>
    </dbReference>
    <dbReference type="OMA" id="WIFWTFI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF94">
        <property type="entry name" value="ETHANOLAMINE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="67" mass="7978" checksum="818F1FF93051ABDD" modified="2019-09-18" version="1">TAVPREVERLYVQVNKFALASHFLWALWALIQNQFSTIDFDFLRYAVIRFNQYFKVKPQVAALEMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3A8</accession>
    <name>A0A4W2D3A8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
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    <gene>
        <name evidence="13" type="primary">LOC113875736</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
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        <property type="gene ID" value="ENSBIXG00000018135.1"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:sensory perception of chemical stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="21"/>
            <end position="42"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="54"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="30"/>
            <end position="290"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="310" mass="34705" checksum="3D2F4066E4D38EBF" modified="2019-09-18" version="1">MAIHRNALRNPGEMVLETISLLQMVVGALGNVILFFHTISPILLGCKMRPTDVILAHVVVANLLIIFSPGIPHIIVAFVLRKPLSGLGCKFVYYIQRVARGSTLCSTCILSTYQSFILTPRTAAWVILRGRAPKAIGPSRCACWLLSFLMNIYVPVTVTGPQGTNNYTDNHGKWFCSSSAPKARFLYLWSISDAVFIGLVVWSSGSMVLLLHRHRQRVRYIHTPTGHYRHPPETRAAHTILMLVVTFVTFYTLDCIFALCIAAFLDFRLSLLHTTNVLASCFPTVSPLLLLLRDPRTRGCCSWKSYDQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7G6</accession>
    <name>A0A4W2D7G6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113888716</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027391547.1">
        <property type="nucleotide sequence ID" value="XM_027535746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7G6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036674.1">
        <property type="protein sequence ID" value="ENSBIXP00000021832.1"/>
        <property type="gene ID" value="ENSBIXG00000024845.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113888716"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
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        <location>
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            <end position="292"/>
        </location>
    </feature>
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        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="312" mass="34798" checksum="A8E1515098008E05" modified="2019-09-18" version="1">MWGGNETESTNFILLGFFPEFRYTTALVSIVLLIYMAAFIGNTLLVLLIWLDSRLHTPMYFLLSQLSLMDLTLTSSIIPKMVANFFSGWQSISFLACGTQIFFSLTVAIGECILITVMCFDRYVAICNPLRYPVIMSPRMCWQVAAMSWAGGALTSSGHTAFTLHFHICSPREIPHFFCEVMAVLRIVCEDISAYEKAVVVTSILVLLLPLSLILSSYILIFLAVLRMNSPEGRNKTLATCSSHLCVVGLYFGPGMCIYMRPGSAKTPKLNQGLFLFGTVLTPLLNPLAYSLRNKEVLCSLKKLMGRFQSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D7U7</accession>
    <name>A0A4W2D7U7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Chloride channel protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Chloride channel protein, skeletal muscle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CLCN1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Voltage-gated chloride channel involved in skeletal muscle excitability. Generates most of the plasma membrane chloride conductance in skeletal muscle fibers, stabilizes the resting membrane potential and contributes to the repolarization phase during action potential firing. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Has a significant open probability at muscle resting potential and is further activated upon membrane depolarization. Permeable to small monovalent anions with ion selectivity for chloride &gt; thiocyanate &gt; bromide &gt; nitrate &gt; iodide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>nitrate(in) = nitrate(out)</text>
            <dbReference type="Rhea" id="RHEA:34923"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>thiocyanate(in) = thiocyanate(out)</text>
            <dbReference type="Rhea" id="RHEA:75347"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Cell membrane</location>
            <location evidence="20">Sarcolemma</location>
            <location evidence="20">T-tubule</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the chloride channel (TC 2.A.49) family. ClC-1/CLCN1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D7U7"/>
    <dbReference type="STRING" id="30522.A0A4W2D7U7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034314.1">
        <property type="protein sequence ID" value="ENSBIXP00000020303.1"/>
        <property type="gene ID" value="ENSBIXG00000023636.1"/>
    </dbReference>
    <dbReference type="OMA" id="KHIGDPE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000003">
        <property type="entry name" value="Chloride channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000027">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000030">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002243">
        <property type="entry name" value="Cl_channel-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF4">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01112">
        <property type="entry name" value="CLCHANNEL1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="49"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="718"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="881"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="887"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="927"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="950"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="988" mass="108814" checksum="2A7ECF18641DF7E8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8M0</accession>
    <name>A0A4W2D8M0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Metal cation symporter ZIP14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Solute carrier family 39 member 14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Zrt- and Irt-like protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">SLC39A14</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Cd(2+)(out) + 2 hydrogencarbonate(out) = Cd(2+)(in) + 2 hydrogencarbonate(in)</text>
            <dbReference type="Rhea" id="RHEA:62256"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:62257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Fe(2+)(out) + 2 hydrogencarbonate(out) = Fe(2+)(in) + 2 hydrogencarbonate(in)</text>
            <dbReference type="Rhea" id="RHEA:62368"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:62369"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>Mn(2+)(out) + 2 hydrogencarbonate(out) = Mn(2+)(in) + 2 hydrogencarbonate(in)</text>
            <dbReference type="Rhea" id="RHEA:62260"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:62261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Zn(2+)(out) + 2 hydrogencarbonate(out) = Zn(2+)(in) + 2 hydrogencarbonate(in)</text>
            <dbReference type="Rhea" id="RHEA:62252"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:62253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Basolateral cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405656.1">
        <property type="nucleotide sequence ID" value="XM_027549855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8M0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050423.1">
        <property type="protein sequence ID" value="ENSBIXP00000022333.1"/>
        <property type="gene ID" value="ENSBIXG00000025228.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039192.1">
        <property type="protein sequence ID" value="ENSBIXP00005024079.1"/>
        <property type="gene ID" value="ENSBIXG00005026763.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897286"/>
    <dbReference type="CTD" id="23516"/>
    <dbReference type="GeneTree" id="ENSGT00940000157986"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="ZIP_Transporter"/>
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        <property type="entry name" value="METAL CATION SYMPORTER ZIP14"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
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    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
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    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
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        <location>
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    <sequence length="490" mass="53806" checksum="77319F12CD15DA6A" modified="2019-09-18" version="1">MELLRPALPSYFLLTLLSIWTAASEARAVSTGMPTISAASFLQNLMHRYGEGDSLTLQQLKALLNHLDVGVGRGNISQPVQGPRNLSTCFSSGELFAAHNLSHQSQIGEREFQEFCPTILQQLDSRACSSENQENEENEQTEEGRPSSVEVWGFGFLSVSLINLASLLGVLVLPCTERAFFSRVLTYFIALSIGTLLSNALFQLIPEAFGFNPMEDYYVSKSAVVFGGFYLFFFTEKILKMLLKQKNEHHHGHSHYTSETLPSQKDQEEGVTEKLQNGDLDHMIPQHCSGELDGKTPVVDEKVIVGSLSVQDLQASQSACHWLKGVRYSDIGTLAWMITLSDGLHNFIDGLAIGASFTVSVFQGISTSVAILCEEFPHELGDFVILLNAGMSLQQALFFNFLSACCCYVGLGFGILAGSHFSANWIFALAGGMFLYISLADMFPEMNEVSQEDERKGSALIPFVIQNLGLLTGFGIMLVLTMYSGHIQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8N5</accession>
    <name>A0A4W2D8N5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZFP36L1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8N5"/>
    <dbReference type="STRING" id="30522.A0A4W2D8N5"/>
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        <property type="protein sequence ID" value="ENSBIXP00000020538.1"/>
        <property type="gene ID" value="ENSBIXG00000023844.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015112.1">
        <property type="protein sequence ID" value="ENSBIXP00005000977.1"/>
        <property type="gene ID" value="ENSBIXG00005029639.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155076"/>
    <dbReference type="OMA" id="YYFRPMS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:1990904">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:14-3-3 protein binding"/>
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    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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    </dbReference>
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        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
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    </dbReference>
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        <property type="term" value="P:cellular response to cAMP"/>
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        <property type="term" value="P:cellular response to epidermal growth factor stimulus"/>
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        <property type="term" value="P:cellular response to fibroblast growth factor stimulus"/>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072091">
        <property type="term" value="P:regulation of stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033077">
        <property type="term" value="P:T cell differentiation in thymus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007635">
        <property type="entry name" value="Tis11B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF53">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="72"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="258"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="323" mass="34671" checksum="61428B080003FECA" modified="2019-09-18" version="1">MTTTLVSATIFDLSEVLCKGNKMLNYSTPSAGGCLLDRKAVGTPAGGGFPRRHSVTLPSSKFHQNQLLSSLKGEPAPALSSRDSRFRDRSFSEGGERLLPPQKQPGSGQVNSSRYKTELCQFAHGIHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRALAGARDLSADRPRLQHSFSFAGFPSAAATAAATGLLDSPTSITPPPILSADDLLGSPTLPDGTNNPFAFSSQELASLFAPSMGLPGGGSPTTFLFRPMSESPHMFDSPPSPQDSLSDQEGYLSSSSSSHSGSDSPTLDNSRRLPIFSRLSISDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9S7</accession>
    <name>A0A4W2D9S7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9S7"/>
    <dbReference type="STRING" id="30522.A0A4W2D9S7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038048.1">
        <property type="protein sequence ID" value="ENSBIXP00000022764.1"/>
        <property type="gene ID" value="ENSBIXG00000025554.1"/>
    </dbReference>
    <dbReference type="OMA" id="YAMVGFF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF91">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN LOC653160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="51"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="346" mass="36003" checksum="B0189494A8F56300" modified="2019-09-18" version="1">MTSLPLASTRSEMNRSVGDLGVGGCRPWDDPARFIVVPAAYALALGLGLPANVAALAVFVRSGRRLGQALRLYLLNLALADVLFTLTLPLWLTYYLGPAHWPFPEAACRAAGAAYYVSTYAAVAFAALISVCRCSSVHRPGPKAAARPALRRRGPARAACAAAWLAGLACAAPSLAAPHALSPGPGGASRCLERGWARAGLAYTTVAFFTAAFLLVLAAYVSLARALAAPSGPGPARAGAHRRAARTMVLGLLLVFALCLAPYHLLLAPWVTGQEVAASREGGGCRATSTLDVLHTLSLALLSLNSCLDPLIYCFSVRRFRRDCWALSCRLGVGAPGAHSASLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBB0</accession>
    <name>A0A4W2DBB0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBB0"/>
    <dbReference type="STRING" id="30522.A0A4W2DBB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036153.1">
        <property type="protein sequence ID" value="ENSBIXP00000021467.1"/>
        <property type="gene ID" value="ENSBIXG00000004967.1"/>
    </dbReference>
    <dbReference type="OMA" id="REGLNQH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000058">
        <property type="entry name" value="ethanolamine-phosphate phospho-lyase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF6">
        <property type="entry name" value="5-PHOSPHOHYDROXY-L-LYSINE PHOSPHO-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="450" mass="49598" checksum="820A07481B52C669" modified="2019-09-18" version="1">MAADQRGKAGTLALRRRLLSNSCRLFFPEDPIKIVRGQGQYMYDEQGAEYIDCINNVAHVGHCHPLVVQAAHEQNQVLNTNSRYLHDNIVDYAQRLSETLPEKLCVFYFLNSGSEANDLALRLARQYTGHWDVVVLDHAYHGHLSSLIDISPYKFRDLDGQKEWVHVAPLPDTYRGPYREDHPNPAVAYASEVKRVVSSAQEKGRKIAAFFAESLPSVGGQIIPPAGYFPEVAGHIRRAGGVFVADEIQVGFGRVGKHFWAFQLQGEDFVPDIVTMGKSIGNGHPVACVATTQAVARAFEATGVEYFNTFGGSPVSCAVGLAVLDVLEKEQLQAHAACVGSFLMELLGQQKAKHPILGDIRGVGLFIGVDLIKDEATRMPATEEADYVVSRLKENYILLSTDGPGRNVLKFKPPMCFSLDNARHVVAKLDAILTDMEAKVRSCETLRLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCY3</accession>
    <name>A0A4W2DCY3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113900863</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCY3"/>
    <dbReference type="STRING" id="30522.A0A4W2DCY3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028596.1">
        <property type="protein sequence ID" value="ENSBIXP00000016606.1"/>
        <property type="gene ID" value="ENSBIXG00000020739.1"/>
    </dbReference>
    <dbReference type="OMA" id="NHCCCIL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15235">
        <property type="entry name" value="7tmA_OR1A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35496" checksum="B159C523C6109A98" modified="2019-09-18" version="1">MERLNQTSSVSEFILLGLSSQPEDQKPLFILFLIMYLVTITGNLLIILAIRSDPQLHTPMYFFLSVLSFTDICFTTTIVPRMLVNFLSHKTISYAGCLTQMYFIYALGNTDSCLLAVMAYDRYVAICDPFHYVTIMSHRRCVLLVAFSCSLPHFHSLLHTLLLNQLTFCDSNIIHNFLCDLSPLIKLSCSPTFVNEIVILIEGSFILVTPFLCITFSYIQILIAVLKIPSASGKRKAFSTCGSHLTVVTLFYGSVFYVYLQPVSTYTVRDHMATIVYTVLSSMLNPFIYSLRNKDLKQGLRKLVGRRKPQAAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEG0</accession>
    <name>A0A4W2DEG0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CD1e molecule</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEG0"/>
    <dbReference type="STRING" id="30522.A0A4W2DEG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040756.1">
        <property type="protein sequence ID" value="ENSBIXP00000024544.1"/>
        <property type="gene ID" value="ENSBIXG00000026674.1"/>
    </dbReference>
    <dbReference type="OMA" id="NHYRIIK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030883">
        <property type="term" value="F:endogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030884">
        <property type="term" value="F:exogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071723">
        <property type="term" value="F:lipopeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048006">
        <property type="term" value="P:antigen processing and presentation, endogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048007">
        <property type="term" value="P:antigen processing and presentation, exogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21029">
        <property type="entry name" value="IgC1_CD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000254">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000002">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF146">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD1E, MEMBRANE-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021453739" evidence="12">
        <location>
            <begin position="20"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="198"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="393" mass="43902" checksum="F43D50EDDC6ABAE8" modified="2019-09-18" version="1">MLLLLPLLPLLFKGLLCHGASTVGLQVPGPHHLAAEESPSFRLIQISSFANHSWTKTQGSGWLGDLQTHGWDSVLASIRFLRPWSQGNFSKEELKNIQAFLQLYLHSFPREVQAYASQFQFEYPFELQVSFGCLIHSGKALETFLNGAYQGLDFLSFQENSWKSSPEAGSRAENVCKVLNHYRVIKEIVQRLLSDTCPRFLAGVLEAGKAELEKQVKPEAWVSKGPSPGPGRLLLVCHVSGFHPKPIWVMWMRGKQKHPGTRRGDVLPNADGTWYLRVTLDVAAGEAAGLSCQVKHSSLGGQDIIIHWGGYSVFWILICLIVIVTLVMLVIVDSLFKKQSSNQDVFSISHPAFPIGAKTQDPRNSGHQLCLAQESWIKNKLLKKWKARLNQLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEV0</accession>
    <name>A0A4W2DEV0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Islet cell autoantigen 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ICA1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DEV0"/>
    <dbReference type="STRING" id="30522.A0A4W2DEV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050681.1">
        <property type="protein sequence ID" value="ENSBIXP00000022832.1"/>
        <property type="gene ID" value="ENSBIXG00000025617.1"/>
    </dbReference>
    <dbReference type="OMA" id="FGPFMDF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07661">
        <property type="entry name" value="BAR_ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000015">
        <property type="entry name" value="Islet cell autoantigen 1, 69kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024114">
        <property type="entry name" value="Islet_autoAg_Ica1/Ica1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006723">
        <property type="entry name" value="Islet_autoAg_Ica1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164:SF3">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04629">
        <property type="entry name" value="ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01237">
        <property type="entry name" value="ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="2">
        <location>
            <begin position="51"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="509" mass="57519" checksum="295CF52C6A55951E" modified="2019-09-18" version="1">MSGHKCSYPWDLHNRYPQDKSVVNKMQQKYWETKQAFIKATGKKEDEHVVASDADLDAKLELFHSIQRTCLDLSKAIVLYQKRICFLSQEENELGKFLRSQGFQDKTRAGKMMQATGKALCFSSQQRLALRNPLCRFHQEVETFRHRAISDTWLTVNRMEQCRTEYRGALLWMKDVSQELDPDLCKQMEKFRKVQTQVRLAKKSFDKLKMDVCQKVDLLGASRCNLLSHMLATYQTTLLHFWEKTSHTMAAIHESFRGYQPYEFTTLKSLQDPMRKLVEKEEKKRIAQQESAEAVAEEPSQLISLEDENQHKESSSFKTEDRKSVLSALDKSCTHNACADELLDMKPEEGACLGPMAGTPEPEGADKDDLLLLSEIFNASSLEEGEFSKEWAAVFGDGRLKEPMPTAAPGELDPRPQAGSGFLPSQLLDQNIKDLQASLEGWSESSVSPSPPWPAPKASSANVNYPAVKEPAKASSDLTAWFSLFADLDPLSNPDAIGKTDKEHELLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DFP6</accession>
    <name>A0A4W2DFP6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DFP6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038341.1">
        <property type="protein sequence ID" value="ENSBIXP00000022977.1"/>
        <property type="gene ID" value="ENSBIXG00000002035.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
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    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="335" mass="38163" checksum="A6B8F12E890AB07B" modified="2019-09-18" version="1">MPANLENQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALFSHASKVMLKILQARLQQYVNCKLPDVQAGFRKVRRTIDQIANIHWIMEKAREFQKNIYFCFTDYAKAFDCVDPNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGDGTTDWFQIGKGVRQGCILSPCLFNLYAEYLMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKTKIMASGPITSWYIDGETVETVSDFIFGGSKITADGDCSHEMKRRLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVEKAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DI92</accession>
    <name>A0A4W2DI92_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="21">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[rhodopsin] + ATP = O-phospho-L-seryl-[rhodopsin] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14594"/>
            <dbReference type="Rhea" id="RHEA-COMP:14595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[rhodopsin] + ATP = O-phospho-L-threonyl-[rhodopsin] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56552"/>
            <dbReference type="Rhea" id="RHEA-COMP:14596"/>
            <dbReference type="Rhea" id="RHEA-COMP:14597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (when prenylated) with PDE6D; this promotes release from membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DI92"/>
    <dbReference type="STRING" id="30522.A0A4W2DI92"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039884.1">
        <property type="protein sequence ID" value="ENSBIXP00000024016.1"/>
        <property type="gene ID" value="ENSBIXG00000001557.1"/>
    </dbReference>
    <dbReference type="OMA" id="YFTEFRV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05607">
        <property type="entry name" value="STKc_GRK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000027">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF12">
        <property type="entry name" value="RHODOPSIN KINASE GRK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0844">Vision</keyword>
    <feature type="domain" description="RGS" evidence="23">
        <location>
            <begin position="62"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="191"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="24">
        <location>
            <begin position="455"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="552" mass="62171" checksum="5026AA733ABF94C0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DMF4</accession>
    <name>A0A4W2DMF4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cell membrane</location>
            <topology evidence="3 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DMF4"/>
    <dbReference type="STRING" id="30522.A0A4W2DMF4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033970.1">
        <property type="protein sequence ID" value="ENSBIXP00000020062.1"/>
        <property type="gene ID" value="ENSBIXG00000002673.1"/>
    </dbReference>
    <dbReference type="OMA" id="WENQTIR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000501">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="316" mass="35204" checksum="77D1DDB6B71BA1C3" modified="2019-09-18" version="1">MGKPNHSGGIFLKGLSGYPRLELLFFVVVLIMYVVILLGNGTLILISILDSHLHTPMYFFLGNLSFLDICYTTVFIPSVLVSFLSVKKTISFSGCAMQMFLGLAMGTTECVLLGMMAFDRYVAICNPLRYSVIMSKGSYVPMAAGSWIVGIVNSTVQTGCVVQLPFCRNNVINHFACEILAVMKLACADISGNEFIMLVATTLFTMTPLLLIIISYTLIIISILRIRSSEGRSKVFSTCSAHLTVVIIFYGTILFMYMKPKSKETLNSDDMDTTDKLVSFFYGVMTPMINPLIYSLRNKDVKEAVKHLLRRKNFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQ30</accession>
    <name>A0A4W2DQ30_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biogenesis of the 60S ribosomal subunit in the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQ30"/>
    <dbReference type="SMR" id="A0A4W2DQ30"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043369.1">
        <property type="protein sequence ID" value="ENSBIXP00000026248.1"/>
        <property type="gene ID" value="ENSBIXG00000028381.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.170:FF:000001">
        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="203" mass="23644" checksum="709ABB3CE9991145" modified="2019-09-18" version="1">MPKAPKGKSVGQEKKVIHPYSRKAAQITRKAHKQEKKEKLKNEKALRLNLIGEKLQWFQNHLDPKKVGYSKRDACELIERYLNRFSSELEQIELRNSFKDRQGRRHCSREAAIRQTLEREQRQYQAHGLEIPDILNADNLKTFREWDFDLKKLPNIKMRKVCASDAVPKKCKKKAVTTIDGDLGELELKDESSDTDEEMTAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DQ69</accession>
    <name>A0A4W2DQ69_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KLC2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388504.1">
        <property type="nucleotide sequence ID" value="XM_027532703.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388505.1">
        <property type="nucleotide sequence ID" value="XM_027532704.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388506.1">
        <property type="nucleotide sequence ID" value="XM_027532705.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388507.1">
        <property type="nucleotide sequence ID" value="XM_027532706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DQ69"/>
    <dbReference type="STRING" id="30522.A0A4W2DQ69"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009634.1">
        <property type="protein sequence ID" value="ENSBIXP00000028657.1"/>
        <property type="gene ID" value="ENSBIXG00000009976.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023013.1">
        <property type="protein sequence ID" value="ENSBIXP00005013256.1"/>
        <property type="gene ID" value="ENSBIXG00005002853.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886484"/>
    <dbReference type="CTD" id="64837"/>
    <dbReference type="GeneTree" id="ENSGT00940000161973"/>
    <dbReference type="OMA" id="SAGEYPT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF2">
        <property type="entry name" value="KINESIN LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="240"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="495"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="85"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="548"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="624" mass="68966" checksum="DE4CF853A9EA4D92" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DRN4</accession>
    <name>A0A4W2DRN4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 204</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Claudin-like protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TMEM204</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Can influence paracellular permeability. Appears to be involved in cell-cell interactions through adherens.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027383759.1">
        <property type="nucleotide sequence ID" value="XM_027527958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRN4"/>
    <dbReference type="SMR" id="A0A4W2DRN4"/>
    <dbReference type="STRING" id="30522.A0A4W2DRN4"/>
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        <property type="protein sequence ID" value="ENSBIXP00000026870.1"/>
        <property type="gene ID" value="ENSBIXG00000029258.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043221.1">
        <property type="protein sequence ID" value="ENSBIXP00005026786.1"/>
        <property type="gene ID" value="ENSBIXG00005002103.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883854"/>
    <dbReference type="CTD" id="79652"/>
    <dbReference type="GeneTree" id="ENSGT00390000015528"/>
    <dbReference type="OMA" id="WKSCWLA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001945">
        <property type="term" value="P:lymph vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030947">
        <property type="term" value="P:regulation of vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051145">
        <property type="term" value="P:smooth muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000008">
        <property type="entry name" value="Transmembrane protein 204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038992">
        <property type="entry name" value="TMEM204"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14627">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14627:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 204"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="chain" id="PRO_5044610859" description="Transmembrane protein 204" evidence="12">
        <location>
            <begin position="26"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
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        </location>
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            <begin position="139"/>
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        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057987"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067890"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083504"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="226" mass="24552" checksum="A1CA0465FE6F5017" modified="2019-09-18" version="1">MTVRKVVATAVLVALVSLVLNNAAAFTPNWVYQTLEDGRRRSVGLWWACWLVERPRGAPGPGARPGPADVRDCEALGWGSEAAGFQESRGTVKLQFDMMRACNLVATAALAAGQLTFVLGLTGLPLLSPDAQCWEEAMAAAFQLASFVLVIGLVTFYRIGPYTSLSWSCYLNIGACLLATLAAAMLIWNVLHRREDCTAPRVIVISRSLTARFRRGLDNDYVESPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT16</accession>
    <name>A0A4W2DT16_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT16"/>
    <dbReference type="STRING" id="30522.A0A4W2DT16"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013571.1">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="271"/>
            <end position="295"/>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="179"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="54"/>
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            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="356" mass="38303" checksum="109D6CD3200710B1" modified="2019-09-18" version="1">MGPPLGHLPSWGPLECTPGPQAWTQAGGPARPVEQTAPPGGRTQAGTLTTAGTGQSRGTLSLPRPQGPHRSWLVCPGARSPLGSAPRPLLQFWDSPFFKSRRFEVHGSPVKQEEDTSQAGCGEGTRMHTHEYAHAPTHTHRYTHRWEFWADDDHQPRILNHENHEGSCLNPRTHFGSDISAANRGGLKGARAQGEWPGSGTRRWSTLQTPTVPAPRWAPQPRLGSHWTSLPLSPVSLGACGGQCRRPRGWGAVTVHSGISGRLCATPGLRWALVAATIVAGVLLTSCLLCTVCCCRRGRHRKRPRDKEAVVLGTTGLTTTTHLVQPELGNVASGPGGAQQWGACSCPWSMTLEARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTP3</accession>
    <name>A0A4W2DTP3_BOBOX</name>
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        <recommendedName>
            <fullName evidence="5">Chibby family member 3</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSBIXG00000025273.1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21533:SF17">
        <property type="entry name" value="PROTEIN CHIBBY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14645">
        <property type="entry name" value="Chibby"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20546" checksum="C2EF9412921A020A" modified="2019-09-18" version="1">LPHIRLWEQLRQFWTDHCSRRFSPRRPPLRRISSMSTFYLLDHQTRQAELGLSYGSPRTRLNDEAFVFRGGRWTSEGQPVWSRPQLPSQTIAGWKAQVQRVRSQVLLEENNYLKLQQELLMDMLTETTARMHLLEKKLDHEAGPGAAPCSWQRKMRKREGAGSLPRLEPRALESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DV04</accession>
    <name>A0A4W2DV04_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protease serine</fullName>
            <ecNumber evidence="11">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DV04"/>
    <dbReference type="SMR" id="A0A4W2DV04"/>
    <dbReference type="STRING" id="30522.A0A4W2DV04"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038261.1">
        <property type="protein sequence ID" value="ENSBIXP00000022912.1"/>
        <property type="gene ID" value="ENSBIXG00000002093.1"/>
    </dbReference>
    <dbReference type="OMA" id="EPNTKMK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017329">
        <property type="entry name" value="Pept_S1A_HAT/DESC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF17">
        <property type="entry name" value="SUPPRESSOR OF TUMORIGENICITY 14 PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037941">
        <property type="entry name" value="TMPRSS11ABCDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 11 13" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="15">
        <location>
            <begin position="41"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="16">
        <location>
            <begin position="182"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037941-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="413" mass="46178" checksum="40977A7366A14C75" modified="2019-09-18" version="1">MDSNTKMKITRCGKISLCILTMVIAILVIGLIVCFVTYGKTYFYHHISFKVNNIDYDSKFAKPYSQEYTDLNNKIISLINETFHGSRLRRQYVTSQVVQVSQAKGKVIIHIVLKFKSYYTNTSAKFRDKIETILRQKLNGKTGSLCIDSSSFRFSDIAKPMAENFLHTCCGHRKITRSGNKIAGGMDAEEGEWPWQASLQQKNVHRCGATLISNSWLVTAAHCFLKARDPKQWNVSFGLLLSDPQIQRSVKDIIIHENYHYPSHKNDIAVVRLSSPVLYTSNIRRACLPETSYAFPSNSDVVVTGWGTLKTDGTSPNILQKGLVKIIDSKICNRKVVYDGAITPGMLCAGFLEGSVDACQGDSGGPLVGEDYKGTWFLAGIVSWGDECALPNKPGVYTRVTHYRDWIMSKTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DVP8</accession>
    <name>A0A4W2DVP8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DVP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009885.1">
        <property type="protein sequence ID" value="ENSBIXP00000028568.1"/>
        <property type="gene ID" value="ENSBIXG00000009827.1"/>
    </dbReference>
    <dbReference type="OMA" id="WHILKEM"/>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="571" mass="66012" checksum="8E3118D244BA5218" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYK7</accession>
    <name>A0A4W2DYK7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking. Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with RHOH. Interacts with the GDP-bound inactive forms of RAB3A, RAB3B, RAB3C, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43; binds RAB3D to a lesser extent. Interacts with DZIP1; this interaction negatively regulates the interaction of GDI2 with GDP-bound RAB8A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYK7"/>
    <dbReference type="STRING" id="30522.A0A4W2DYK7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040534.1">
        <property type="protein sequence ID" value="ENSBIXP00000024404.1"/>
        <property type="gene ID" value="ENSBIXG00000026569.1"/>
    </dbReference>
    <dbReference type="OMA" id="TSMQEVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000014">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF1">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 13" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="230" mass="26297" checksum="A4A9F0753415E8FF" modified="2019-09-18" version="1">MGKQWKTVSDFIFLGSKITECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGAPPASMGRGRDWNVDLIPKFLMANGQLVKMLLFTEVTRYLDFKVNEGSFFYKGGKIYKVPSTEAEALASSLMGLFGKRRFRKFLIYVANFDEKDPRTFEGIDPKKTSMQEVCKKFDLGQDVIDFTGHALALYRTDDYLDQPCCETINRIKLYSESLRHKNDIYGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYN0</accession>
    <name>A0A4W2DYN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYN0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043672.1">
        <property type="protein sequence ID" value="ENSBIXP00000031913.1"/>
        <property type="gene ID" value="ENSBIXG00000014360.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="30"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="539" mass="57368" checksum="897AA8EDBC159DA8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYV2</accession>
    <name>A0A4W2DYV2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxisome proliferator-activated receptor alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nuclear receptor subfamily 1 group C member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PPARA</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with NCOA3 coactivator. Interacts with CITED2; the interaction stimulates its transcriptional activity. Also interacts with PPARBP in vitro. Interacts with AKAP13, LPIN1, PRDM16 and coactivator NCOA6. Interacts with ASXL1 and ASXL2. Interacts with PER2. Interacts with SIRT1; the interaction seems to be modulated by NAD(+) levels. Interacts with CRY1 and CRY2. In hepatocytes, interacts with PAQR3 and HUWE1; the interactions promote PPARA poylubiquitination and HUWE1-mediated degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396297.1">
        <property type="nucleotide sequence ID" value="XM_027540496.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027396298.1">
        <property type="nucleotide sequence ID" value="XM_027540497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYV2"/>
    <dbReference type="SMR" id="A0A4W2DYV2"/>
    <dbReference type="STRING" id="30522.A0A4W2DYV2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006303.1">
        <property type="protein sequence ID" value="ENSBIXP00000029697.1"/>
        <property type="gene ID" value="ENSBIXG00000011423.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005012488.1">
        <property type="protein sequence ID" value="ENSBIXP00005028879.1"/>
        <property type="gene ID" value="ENSBIXG00005000246.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892102"/>
    <dbReference type="CTD" id="5465"/>
    <dbReference type="GeneTree" id="ENSGT00940000157097"/>
    <dbReference type="OMA" id="TNHPDNI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070166">
        <property type="term" value="P:enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008544">
        <property type="term" value="P:epidermis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032099">
        <property type="term" value="P:negative regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010887">
        <property type="term" value="P:negative regulation of cholesterol storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900016">
        <property type="term" value="P:negative regulation of cytokine production involved in inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903944">
        <property type="term" value="P:negative regulation of hepatocyte apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903038">
        <property type="term" value="P:negative regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010745">
        <property type="term" value="P:negative regulation of macrophage derived foam cell differentiation"/>
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    <keyword evidence="10 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
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    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="20">
        <location>
            <begin position="101"/>
            <end position="175"/>
        </location>
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        <location>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="470" mass="52428" checksum="9F36D23325D247C7" modified="2019-09-18" version="1">MEMVDTESPIGPLSPLEADDLESPLSADFLQEMGTIQEISQSIGEDSSGSFSFTEYQYLGSGPGSDGSVITDTLSPASSPSSVSYPAVPGSAEESSSIALNIECRICGDKASGYHYGVHACEGCKGFFRRTIRLKLVYDKCDRSCKIQKKNRNKCQYCRFHKCLSVGMSHNAIRFGRMPRSEKAKLKAEILTCEHDLEDSETADLKSLAKRIYEAYLKNFNMNKIKARVILAGKTNNNPPFVIHDMETLCMAEKTLVAKLVANGIQNKEAEVRIFHCCQCTSVETVTELTEFAKSIPGFANLDLNDQVTLLKYGVYEAIFAMLSSVMNKDGMLVAYGNGFITREFLKSLRKPFCDIMEPKFDFAMKFNALELDDSDISLFVAAIICCGDRPGLLNVGHIEKMQEGIVHVLKLHLQNNHPDDVFLFPKLLQKMADLRQLVTEHAQLVQVIKKTESDAALHPLLQEIYRDMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZ50</accession>
    <name>A0A4W2DZ50_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-hydroxytryptamine receptor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZ50"/>
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    <dbReference type="STRING" id="30522.A0A4W2DZ50"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051688.1">
        <property type="protein sequence ID" value="ENSBIXP00000024611.1"/>
        <property type="gene ID" value="ENSBIXG00000026735.1"/>
    </dbReference>
    <dbReference type="OMA" id="CRDRQLX"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032098">
        <property type="term" value="P:regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="7tmA_5-HT4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5-hydroxytryptamine receptor 4"/>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001520">
        <property type="entry name" value="5HT4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01059">
        <property type="entry name" value="5HT4RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
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            <end position="295"/>
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        <location>
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            <end position="329"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="36"/>
            <end position="326"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="402" mass="45427" checksum="422FBA1D1348FDDE" modified="2019-09-18" version="1">MDELDANVSSKAGFRSVEKVVLLTFLSAVILMAILGNLLVMAAVCRDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELVQDIWIYGEMFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPMFISFLPIMQGWNNIGIIDLERISKPRLGQDLHVIEKRKFNQNSNSTYCIFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAHQIQMLQRAGAPTEGRLQPTDQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNIVDPFVDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTINGSTHVLRDAVECGGQWESQSHPPASSPLVAAQPSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZW9</accession>
    <name>A0A4W2DZW9_BOBOX</name>
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        <submittedName>
            <fullName evidence="11">Integrin alpha FG-GAP repeat containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ITFG1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIP family.</text>
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        <property type="gene ID" value="ENSBIXG00000001757.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005007827.1"/>
        <property type="gene ID" value="ENSBIXG00005013019.1"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="entry name" value="Integrin_alpha_N"/>
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        <property type="entry name" value="Tip"/>
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        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN HOMOLOG"/>
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        <property type="entry name" value="Integrin alpha N-terminal domain"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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            <end position="33"/>
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            <end position="589"/>
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            <end position="559"/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032061.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="611" mass="67804" checksum="17B15B3B09C6C241" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1N7</accession>
    <name>A0A4W2E1N7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ectodermal-neural cortex 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ENC1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027375811.1">
        <property type="nucleotide sequence ID" value="XM_027520010.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2E1N7"/>
    <dbReference type="STRING" id="30522.A0A4W2E1N7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018301.1">
        <property type="protein sequence ID" value="ENSBIXP00000032767.1"/>
        <property type="gene ID" value="ENSBIXG00000015424.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047098.1">
        <property type="protein sequence ID" value="ENSBIXP00005014310.1"/>
        <property type="gene ID" value="ENSBIXG00005002964.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878875"/>
    <dbReference type="CTD" id="8507"/>
    <dbReference type="GeneTree" id="ENSGT00950000182983"/>
    <dbReference type="OMA" id="KMSVSMH"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18515">
        <property type="entry name" value="BACK_KLHL37_ENC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18267">
        <property type="entry name" value="BTB_POZ_KLHL37_ENC1"/>
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    </dbReference>
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        <property type="entry name" value="Ectoderm-neural cortex protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000004">
        <property type="entry name" value="Ectoderm-neural cortex protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ectoderm-neural cortex protein 1"/>
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    </dbReference>
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        <property type="entry name" value="Kelch-like family member 12"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="BACK"/>
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        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
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        <property type="entry name" value="ENC1_BACK"/>
    </dbReference>
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        <property type="entry name" value="ENC1_BTB_POZ_dom"/>
    </dbReference>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF496">
        <property type="entry name" value="ECTODERM-NEURAL CORTEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="46"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="589" mass="66090" checksum="EF639330E2DCA12A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7B3</accession>
    <name>A0A4W2E7B3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113904851</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E7B3"/>
    <dbReference type="STRING" id="30522.A0A4W2E7B3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023201.1">
        <property type="protein sequence ID" value="ENSBIXP00000034889.1"/>
        <property type="gene ID" value="ENSBIXG00000002171.1"/>
    </dbReference>
    <dbReference type="OMA" id="MAIRNHP"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR6B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="42"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="319" mass="35087" checksum="83C0200BEAD35CD2" modified="2019-09-18" version="1">MERKNQSGRVSEFVLLGFPAPVPLRALLFALSLLAYVLVLTENILIIVAIRSHPSLHKPMYFFLANMSFLEIWYVTVTIPKMLAGFVGSKRGRGQLISFEGCMTQLCFFLGLGCTECVLLAVMAYDRYVAICHPLHYPVIVSGQLCVQLSAGSWAGGFGISMVKVFLISRLSYCGPNIINHFFCDVSPLLNLSCTDMSTAELTDFVLAIFILLGPLSVTGASYMAITSAVMRIPSAAGRHKAFSTCASHLTVVVIFYAASIFIYARPKALSAFDTNKLVSVLYAVIVPLLNPIIYCLRNQEVKRALRHTLHLHQGHDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7U7</accession>
    <name>A0A4W2E7U7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ethylmalonyl-CoA decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Enoyl-CoA hydratase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Methylmalonyl-CoA decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ECHDC1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Decarboxylates ethylmalonyl-CoA, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading. Acts preferentially on (S)-ethylmalonyl-CoA but also has some activity on the (R)-isomer. Also has methylmalonyl-CoA decarboxylase activity at lower level.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-ethylmalonyl-CoA + H(+) = butanoyl-CoA + CO2</text>
            <dbReference type="Rhea" id="RHEA:59540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:85316"/>
            <dbReference type="EC" id="4.1.1.94"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:59541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2S)-ethylmalonyl-CoA + H(+) = butanoyl-CoA + CO2</text>
            <dbReference type="Rhea" id="RHEA:32131"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:60909"/>
            <dbReference type="EC" id="4.1.1.94"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:32132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(S)-methylmalonyl-CoA + H(+) = propanoyl-CoA + CO2</text>
            <dbReference type="Rhea" id="RHEA:61340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57327"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="EC" id="4.1.1.94"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:61341"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.94" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E7U7"/>
    <dbReference type="STRING" id="30522.A0A4W2E7U7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018515.1">
        <property type="protein sequence ID" value="ENSBIXP00000032870.1"/>
        <property type="gene ID" value="ENSBIXG00000007074.1"/>
    </dbReference>
    <dbReference type="OMA" id="FTICRPE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004492">
        <property type="term" value="F:methyl/ethyl malonyl-CoA decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000040">
        <property type="entry name" value="Ethylmalonyl-CoA decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF27">
        <property type="entry name" value="ETHYLMALONYL-COA DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00036343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038883"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039903"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00042052"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042182"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047446"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="306" mass="33541" checksum="294C6A3A53186C88" modified="2019-09-18" version="1">MELKQEMASLLKTSPNAVKKRLLHQIGLSLYNTSHGFHEEEVKKKLEQFPGGSIDLQKENSGIGILTLNNPSKMNAFSGVMMLQLLEKVIELENWTEGKGLIIRGAKNTFSSGSDLNAVKALGTPEDGMAVCMFMQNTLTRFMRLPLISVALVQGRALGGGAEVTTACDFRLMTTQSEIRFVHKEMGIIPSWGGATRLVEIIGGRQALKVLSGALKLDSEKALNIGMVDDILPSSDETECLKEAQEWLQQFIKGPPEVIRALKKSVSSCKELCLEEALQRERDILGTVWGGPANLEAVARKGKFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E820</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="common">Hybrid cattle</name>
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            <taxon>Mammalia</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="Ensembl" id="ENSBIXP00000027844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="566" mass="64700" checksum="4B372CE957A71154" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8E7</accession>
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            <fullName evidence="2">WAP domain-containing protein</fullName>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5021301093" description="WAP domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="78" mass="8893" checksum="11DBF15EA6A76D89" modified="2019-09-18" version="1">MRAQALLPILLLCVLLLQARGRQHSQKTNQKQQPPEIKQCEKRPKIYMCKIPCTDDLECQANNICCSTFCGNICMNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAV0</accession>
    <name>A0A4W2EAV0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein capicua homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CIC</name>
    </gene>
    <organism evidence="14 15">
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional repressor which plays a role in development of the central nervous system (CNS). In concert with ATXN1 and ATXN1L, involved in brain development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Found in a complex with ATXN1 and ATXN1L.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with ATXN1.</text>
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    <dbReference type="STRING" id="30522.A0A4W2EAV0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021650.1">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="High mobility group box domain"/>
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        <property type="entry name" value="CC-Dev_Transcription_Reg"/>
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        <property type="entry name" value="HMG-box_Cic-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
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    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH_Cic_C"/>
    </dbReference>
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        <property type="entry name" value="HMG-BOX TRANSCRIPTION FACTOR BBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13059:SF13">
        <property type="entry name" value="PROTEIN CAPICUA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16090">
        <property type="entry name" value="DUF4819"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25981">
        <property type="entry name" value="HTH_Cic_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="13">
        <location>
            <begin position="1106"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="11">
        <location>
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        </location>
    </feature>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="341"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="12">
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1232"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1288"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1375"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1591"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1690"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1790"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2029"/>
            <end position="2051"/>
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            <begin position="40"/>
            <end position="74"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="152"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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        </location>
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            <end position="925"/>
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            <begin position="945"/>
            <end position="967"/>
        </location>
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        <location>
            <begin position="1002"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1135"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="779"/>
            <end position="792"/>
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            <end position="803"/>
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            <begin position="815"/>
            <end position="833"/>
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        <location>
            <begin position="1026"/>
            <end position="1038"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1046"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1267" mass="137214" checksum="08E2F6E82FC8D0C1" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ED48</accession>
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        <submittedName>
            <fullName evidence="6">NUMB like endocytic adaptor protein</fullName>
        </submittedName>
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        <name evidence="6" type="primary">NUMBL</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the process of neurogenesis.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2ED48"/>
    <dbReference type="STRING" id="30522.A0A4W2ED48"/>
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        <property type="protein sequence ID" value="ENSBIXP00000033858.1"/>
        <property type="gene ID" value="ENSBIXG00000016690.1"/>
    </dbReference>
    <dbReference type="OMA" id="PWMSRSA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="PTB_Numb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000031">
        <property type="entry name" value="protein numb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Numb/numb-like"/>
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    <dbReference type="InterPro" id="IPR010449">
        <property type="entry name" value="Numb_domain"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PTB/PI_dom"/>
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    <dbReference type="PANTHER" id="PTHR47368">
        <property type="entry name" value="NUMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF4">
        <property type="entry name" value="NUMB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06311">
        <property type="entry name" value="NumbF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Numb/numb-like"/>
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    </dbReference>
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        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="78"/>
            <end position="203"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="534"/>
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        <location>
            <begin position="545"/>
            <end position="560"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033858.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="596" mass="63316" checksum="D6B1FF96F32091DF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEI3</accession>
    <name>A0A4W2EEI3_BOBOX</name>
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        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein kinase DCLK2</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CaMK-like CREB regulatory kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Doublecortin-like and CAM kinase-like 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Doublecortin-like kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">DCLK2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027369215.1">
        <property type="nucleotide sequence ID" value="XM_027513414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EEI3"/>
    <dbReference type="STRING" id="30522.A0A4W2EEI3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004600.1">
        <property type="protein sequence ID" value="ENSBIXP00000030259.1"/>
        <property type="gene ID" value="ENSBIXG00000012152.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054214.1">
        <property type="protein sequence ID" value="ENSBIXP00005045674.1"/>
        <property type="gene ID" value="ENSBIXG00005029213.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875052"/>
    <dbReference type="CTD" id="166614"/>
    <dbReference type="GeneTree" id="ENSGT00940000154895"/>
    <dbReference type="OMA" id="LMTECKV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17141">
        <property type="entry name" value="DCX1_DCLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17069">
        <property type="entry name" value="DCX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14184">
        <property type="entry name" value="STKc_DCKL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000066">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000057">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000001">
        <property type="entry name" value="serine/threonine-protein kinase DCLK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000002">
        <property type="entry name" value="serine/threonine-protein kinase DCLK2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Doublecortin" evidence="22">
        <location>
            <begin position="72"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin" evidence="22">
        <location>
            <begin position="196"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="409"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="299"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="718"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="734"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="767" mass="83642" checksum="CD4FC5981FC2E0AF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFQ1</accession>
    <name>A0A4W2EFQ1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GRHPR</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFQ1"/>
    <dbReference type="SMR" id="A0A4W2EFQ1"/>
    <dbReference type="STRING" id="30522.A0A4W2EFQ1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025800.1">
        <property type="protein sequence ID" value="ENSBIXP00000036010.1"/>
        <property type="gene ID" value="ENSBIXG00000019379.1"/>
    </dbReference>
    <dbReference type="OMA" id="PHIAWAY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF137">
        <property type="entry name" value="GLYOXYLATE REDUCTASE_HYDROXYPYRUVATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="9"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="117"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="328" mass="35770" checksum="0394EDDB6D04A3FB" modified="2019-09-18" version="1">MRPVRLMKVFITRRIPPEGSAALARAADCEVEQWDSDEPIPREDLEQRMAGAQGLLCLLSDRIDKKLLDAAGANLKVISTMSVGVDHLALDEIKKRGIRVGYTPGVLTDATAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWMCGHGLSQSTVGIVGLGRIGQAIARRLKPFGVRRFLYTGRQPRPQEAAEFQAEFVSTPKLAAESDFIVVACSLTPATRGLCNKDFFQWMKKTAVFVNISRGEVVDQDDLYQALASGQIAAAGLDVTTPEPLPTNHPLLTLKNCVILPHIGSATHRTRNIMSVLAADNLLAGLRGEPMPSELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGS3</accession>
    <name>A0A4W2EGS3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alkaline phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGS3"/>
    <dbReference type="STRING" id="30522.A0A4W2EGS3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027352.1">
        <property type="protein sequence ID" value="ENSBIXP00000036664.1"/>
        <property type="gene ID" value="ENSBIXG00000003673.1"/>
    </dbReference>
    <dbReference type="OMA" id="MAEHINQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57262">
        <property type="entry name" value="Leech antihemostatic proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51252">
        <property type="entry name" value="ANTISTASIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021290507" description="Cysteine-rich motor neuron 1 protein" evidence="11">
        <location>
            <begin position="35"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="939"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="14">
        <location>
            <begin position="33"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="12">
        <location>
            <begin position="334"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="12">
        <location>
            <begin position="401"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Antistasin-like" evidence="13">
        <location>
            <begin position="469"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Antistasin-like" evidence="13">
        <location>
            <begin position="505"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Antistasin-like" evidence="13">
        <location>
            <begin position="539"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Antistasin-like" evidence="13">
        <location>
            <begin position="567"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="12">
        <location>
            <begin position="606"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="12">
        <location>
            <begin position="677"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="12">
        <location>
            <begin position="751"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="12">
        <location>
            <begin position="817"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1035" mass="113874" checksum="1DF2652618C052C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELE1</accession>
    <name>A0A4W2ELE1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EEF1A lysine methyltransferase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Methyltransferase-like protein 21B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein-lysine methyltransferase METTL21B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EEF1AKMT3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein-lysine methyltransferase that selectively mono-, di- and trimethylates 'Lys-165' of the translation elongation factors EEF1A1 and EEF1A2 in an aminoacyl-tRNA and GTP-dependent manner. EEF1A1 methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:51737"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54200"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="Rhea" id="RHEA-COMP:13827"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:54201"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54196"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="Rhea" id="RHEA-COMP:13827"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:54197"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with members of the heat shock protein 70 and 90 families and of the TCP-1 chaperonin family, as well as with HSPD1, STIP1 and tubulin; at least some of these proteins may be methylation substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the methyltransferase superfamily. METTL21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398195.1">
        <property type="nucleotide sequence ID" value="XM_027542394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELE1"/>
    <dbReference type="SMR" id="A0A4W2ELE1"/>
    <dbReference type="STRING" id="30522.A0A4W2ELE1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026927.1">
        <property type="protein sequence ID" value="ENSBIXP00000036487.1"/>
        <property type="gene ID" value="ENSBIXG00000003607.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006511.1">
        <property type="protein sequence ID" value="ENSBIXP00005030870.1"/>
        <property type="gene ID" value="ENSBIXG00005011058.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892901"/>
    <dbReference type="CTD" id="25895"/>
    <dbReference type="GeneTree" id="ENSGT00940000161297"/>
    <dbReference type="OMA" id="HRDDKQN"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000160">
        <property type="entry name" value="EEF1A lysine methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="226" mass="24972" checksum="AB328D2D934F1992" modified="2019-09-18" version="1">MADSRPDPESEPDSVFPREVGLFADCYSEKSRFCFCGHVLNITENFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGGDVTITDLPLVLEQIQGNVQANVPPGGRAQVRALSWGIDQHVFPGDYDLVLGADIVYLEPTFPLLLGTLRHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQLELAQRDEDENVNIYRARHRGPRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELS4</accession>
    <name>A0A4W2ELS4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM200 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELS4"/>
    <dbReference type="STRING" id="30522.A0A4W2ELS4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029144.1">
        <property type="protein sequence ID" value="ENSBIXP00000037185.1"/>
        <property type="gene ID" value="ENSBIXG00000003843.1"/>
    </dbReference>
    <dbReference type="OMA" id="GMEQGTR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018787">
        <property type="entry name" value="DUF2371_TMEM200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31815">
        <property type="entry name" value="AGAP005329-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31815:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 200B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10177">
        <property type="entry name" value="DUF2371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="388" mass="41860" checksum="18982059EB0FE0F5" modified="2019-09-18" version="1">MGGWRRGRGLFAEFQEILRKVCRWFWSERSSQSLAQVCLAAASVLTEEVWKEGAGRKRLESVTTPTTTTTFLRCERPDGGEMTAGSPGDCGEVRRSPEGRVSRLGRRLGRRRRARSPPEPLRVRARLRLRSPSGAFAALGALVVLVGMGIAVAGYWPHRAGVPGPRAANSSAPPLSELRREGRGAGRAHGPHERLRLLGPVVMGVGLFVFICANTLLYENRDLETRRLRQGVLRAQALRPPDGPGWDCALLPSPGPRTPRAVGCVEPESWDLSPRRGTSPVPSVRSLRSEPANPRLGLPALLNSYPLKGSGLHPPWGPRPQAGHVIITVQPSGSCIEHSKSLDLGLGELLLGAPAARDCAHRSWPRLDRLSLGGYAKLGAGGDLGARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMB8</accession>
    <name>A0A4W2EMB8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113893560</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMB8"/>
    <dbReference type="STRING" id="30522.A0A4W2EMB8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030446.1">
        <property type="protein sequence ID" value="ENSBIXP00000038005.1"/>
        <property type="gene ID" value="ENSBIXG00000021704.1"/>
    </dbReference>
    <dbReference type="OMA" id="DFCESHI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15229">
        <property type="entry name" value="7tmA_OR8S1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="311" mass="34554" checksum="0EA13848CB7999C6" modified="2019-09-18" version="1">MTLGNHTIVTQFLLLGLSTDPHIQALLFVLFLEIYLLTLMGNLMMLLVIRADSHLHMPMFFFLSHLSLLDLCLSSVTVPKMLKDLLSETKTISLRGCLAQGFFVLITAGTECFLLSAMAYDRYAAICHPLLYGQMMKKQLCVQLVWGSWGLASLNAFINTLLAANLDFCENHTISHYSCEVPSLFPLSCSDVSINLIVLLCSSLMHGFGTLLPIVFSYAHIVSTILSISSTSGRSKTFSTCSSHLTAVSFFFGSGFLRYLMPTSGSPLELIFSVQYGVVTPMVNPLIYSLRNKEVKAAVRRTLGKYMRWSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPL6</accession>
    <name>A0A4W2EPL6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TUBB4A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027403253.1">
        <property type="nucleotide sequence ID" value="XM_027547452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPL6"/>
    <dbReference type="SMR" id="A0A4W2EPL6"/>
    <dbReference type="STRING" id="30522.A0A4W2EPL6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048749.1">
        <property type="protein sequence ID" value="ENSBIXP00000038836.1"/>
        <property type="gene ID" value="ENSBIXG00000022728.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005740.1">
        <property type="protein sequence ID" value="ENSBIXP00005031121.1"/>
        <property type="gene ID" value="ENSBIXG00005001091.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895802"/>
    <dbReference type="CTD" id="10382"/>
    <dbReference type="GeneTree" id="ENSGT00940000161972"/>
    <dbReference type="OMA" id="CFPAGGN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033269">
        <property type="term" value="C:internode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031115">
        <property type="term" value="P:negative regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
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    </dbReference>
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        <property type="entry name" value="Beta-tubulin_BS"/>
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        <property type="entry name" value="Beta_tubulin"/>
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        <property type="entry name" value="Tub_FtsZ_C"/>
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    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
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    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
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        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="444" mass="49586" checksum="F7429D057C11A3F5" modified="2019-09-18" version="1">MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEAEEEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPY2</accession>
    <name>A0A4W2EPY2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prefoldin subunit 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PFDN5</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPY2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032362.1">
        <property type="protein sequence ID" value="ENSBIXP00000038816.1"/>
        <property type="gene ID" value="ENSBIXG00000006225.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018497.1">
        <property type="protein sequence ID" value="ENSBIXP00005034046.1"/>
        <property type="gene ID" value="ENSBIXG00005014918.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008783"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990113">
        <property type="term" value="P:RNA polymerase I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990115">
        <property type="term" value="P:RNA polymerase III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23157">
        <property type="entry name" value="Prefoldin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000011">
        <property type="entry name" value="prefoldin subunit 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="109" mass="12395" checksum="90AB656E8F463144" modified="2019-09-18" version="1">MAQSVNITELNLPQLEMLKNQLDQMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTTLGAAQATAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EQ35</accession>
    <name>A0A4W2EQ35_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LRRC18</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in the regulation of spermatogenesis and sperm maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EQ35"/>
    <dbReference type="STRING" id="30522.A0A4W2EQ35"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048896.1">
        <property type="protein sequence ID" value="ENSBIXP00000039040.1"/>
        <property type="gene ID" value="ENSBIXG00000022961.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005056153.1">
        <property type="protein sequence ID" value="ENSBIXP00005046410.1"/>
        <property type="gene ID" value="ENSBIXG00005031236.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156026"/>
    <dbReference type="OMA" id="DSQEDWR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000246">
        <property type="entry name" value="Leucine rich repeat containing 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF42">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 18-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="32"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00071471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005046410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="261" mass="29387" checksum="3314D82EAA081426" modified="2019-09-18" version="1">MAKGAKGPKGKKITLAVAKNCIKITFDGKKRLDLSKMGITTFPKCILRLTDVDELDLSRNLIKKIPDAISKFQNLRWLDLHSNYIDKLPETIGQMTSLLYLNVSNNRLTTNGLPVELNQLKNIRTVNLGLNHLDSVPTTLGALKELHEVGLHDNLLSNIPNSISKLPKLKKLNTKRNPFPKAESSDMFMDSIRRLDNLHLVEEKDLCGTCLKKCQQARDKLNKIKSMATTIPRKAIFSTLVSPNSMAKESQEDWRMRSTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ET67</accession>
    <name>A0A4W2ET67_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-alpha-acetyltransferase 35, NatC auxiliary subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein MAK10 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NAA35</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Auxillary component of the N-terminal acetyltransferase C (NatC) complex which catalyzes acetylation of N-terminal methionine residues. N-terminal acetylation protects proteins from ubiquitination and degradation by the N-end rule pathway. Involved in regulation of apoptosis and proliferation of smooth muscle cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of NAA35, NAA38 and NAA30.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027405948.1">
        <property type="nucleotide sequence ID" value="XM_027550147.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405949.1">
        <property type="nucleotide sequence ID" value="XM_027550148.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027405950.1">
        <property type="nucleotide sequence ID" value="XM_027550149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ET67"/>
    <dbReference type="STRING" id="30522.A0A4W2ET67"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035577.1">
        <property type="protein sequence ID" value="ENSBIXP00000040226.1"/>
        <property type="gene ID" value="ENSBIXG00000024270.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047403.1">
        <property type="protein sequence ID" value="ENSBIXP00005014933.1"/>
        <property type="gene ID" value="ENSBIXG00005003065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897408"/>
    <dbReference type="CTD" id="60560"/>
    <dbReference type="GeneTree" id="ENSGT00390000002445"/>
    <dbReference type="OMA" id="QMEWIVQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048659">
        <property type="term" value="P:smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="9">
        <location>
            <begin position="46"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like N-terminal" evidence="9">
        <location>
            <begin position="146"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="10">
        <location>
            <begin position="338"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="548"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="725" mass="83566" checksum="F2A6866503C9028D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXC0</accession>
    <name>A0A4W2EXC0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EXC0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050909.1">
        <property type="protein sequence ID" value="ENSBIXP00000041668.1"/>
        <property type="gene ID" value="ENSBIXG00000025812.1"/>
    </dbReference>
    <dbReference type="OMA" id="HEMGIPD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="340" mass="39045" checksum="D404DACFC7902C86" modified="2019-09-18" version="1">MLKILQARLQQYVNRELPGVQACFRKGRGTRDQIANIRWIMEKAREFQKSIYFCFIDYAKAFNCVDHNTLWKILHEMGIPDHLICLLRNLYTGQEATVRTGHGTTDWFQIGKGVRQGCILSPYLFNLYAEYIMRDAGLEETQAGIKIAGRNSNNLRYADDTTLMAESEEELKSLLMKVKVENEKVGLKLNIQKTKIMASGPTTSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKAMTNLDSILKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAERQRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGRPVLWPPHAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXH8</accession>
    <name>A0A4W2EXH8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Toll-like receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 17">Belongs to the Toll-like receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EXH8"/>
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        <property type="protein sequence ID" value="ENSBIXP00000041798.1"/>
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        <property type="term" value="P:innate immune response"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cys-rich_flank_reg_C"/>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
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        <property type="entry name" value="Toll-like_receptor"/>
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        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOLL-LIKE RECEPTOR 6"/>
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        <property type="entry name" value="LRR_8"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Toll-like_receptor"/>
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        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
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        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
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        <property type="entry name" value="LRR"/>
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    </dbReference>
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        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15 17" id="KW-0391">Immunity</keyword>
    <keyword evidence="14 15 17" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3 15 17" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12 15 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="585"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="18">
        <location>
            <begin position="640"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037595"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR037595-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="796" mass="91423" checksum="A539952CCE76FEDF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1C5</accession>
    <name>A0A4W2F1C5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Adhesion G protein-coupled receptor L1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">ADGRL1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GAIN_dom_sf"/>
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    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
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        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
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        <property type="entry name" value="GPCR_2_extracellular_dom"/>
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        <property type="entry name" value="GPCR_2_latrophilin"/>
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    <dbReference type="InterPro" id="IPR003334">
        <property type="entry name" value="GPCR_2_latrophilin_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
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        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
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        <property type="entry name" value="GPS"/>
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        <property type="entry name" value="Latrophilin-1_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
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        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="Olfac-like_dom"/>
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        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR L1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02354">
        <property type="entry name" value="Latrophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01444">
        <property type="entry name" value="LATROPHILIN"/>
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    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81321">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer, non-covalently linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 22">Belongs to the SCF family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00000020665.1"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:filopodium"/>
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        <property type="term" value="C:lamellipodium"/>
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        <property type="term" value="F:cytokine activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:stem cell factor receptor binding"/>
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        <property type="term" value="P:male gonad development"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:Ras protein signal transduction"/>
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    </dbReference>
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        <property type="entry name" value="SCF"/>
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        <property type="entry name" value="KIT LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="19" id="KW-0206">Cytoskeleton</keyword>
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    <keyword evidence="16 22 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
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    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 25" id="KW-0732">Signal</keyword>
    <keyword evidence="11 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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        <location>
            <begin position="26"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
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            <end position="238"/>
        </location>
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        </location>
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        </location>
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        <source>
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    <evidence type="ECO:0000313" key="28">
        <source>
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    <sequence length="249" mass="27740" checksum="9D0279F590342593" modified="2019-09-18" version="1">MKKTQTWIITCIYLQLLLFNPLVHTQGICSNRVTDDVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVEQLSVSLTDLLDKFSNISEGLSNYCIIDKLVKIVDDLVECMEEHSSENVKKSSKSPEPRQFTPEKFFGIFNKSIDAFKDLEIVASKMSECVISSTSSPEKDSRVSVTKPFMLPPVAASSLRNDSSSSNRKASNSIEDSSLQWAAVALPAFFSLVIGFAFGAFYWKAGNSSCLLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="MAMMAGLOBIN-B"/>
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        <property type="entry name" value="MAMMAGLOBIN-RELATED"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005004646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="99" mass="11081" checksum="95D4E2629F5A41F1" modified="2019-09-18" version="1">MKLVTVLMLVALPLYCYAGSSGCSLLDNVVEKSVDPTVSKDEYRAYLKDFAQTDVEKKAADELKQCFLQQSNETLANFKQMLVISASSDVLLQPRDRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FUK1</accession>
    <name>A0A4W2FUK1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Shisa like 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SHISAL2B</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027376065.1">
        <property type="nucleotide sequence ID" value="XM_027520264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FUK1"/>
    <dbReference type="SMR" id="A0A4W2FUK1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044009.1">
        <property type="protein sequence ID" value="ENSBIXP00000009226.1"/>
        <property type="gene ID" value="ENSBIXG00000014938.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005017282.1">
        <property type="protein sequence ID" value="ENSBIXP00005009354.1"/>
        <property type="gene ID" value="ENSBIXG00005001879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879020"/>
    <dbReference type="CTD" id="100132916"/>
    <dbReference type="GeneTree" id="ENSGT00940000161622"/>
    <dbReference type="OMA" id="RYCCGFA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF2">
        <property type="entry name" value="PROTEIN SHISA-LIKE-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005009354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="161" mass="17880" checksum="88799AF875A132CA" modified="2019-09-18" version="1">MSEASRVCSGYYSLNHSFVEPFQCPRRGEGATLLYCCGFADLKYCCSEPGSYFPYKHSYMWSLSIGALIGLGIAALVLLAFVISVCVLCYLFLYTKPQRLDTGLKLQHLEAVSTQEGNSNRKSKAPRSNAASNSTNETFYEADDIIQEKTMDTTQINTAYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GAT1</accession>
    <name>A0A4W2GAT1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="22">ADAM metallopeptidase with thrombospondin type 1 motif 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ADAMTS19</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GAT1"/>
    <dbReference type="STRING" id="30522.A0A4W2GAT1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034515.1">
        <property type="protein sequence ID" value="ENSBIXP00000020448.1"/>
        <property type="gene ID" value="ENSBIXG00000023764.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027611.1">
        <property type="protein sequence ID" value="ENSBIXP00005015722.1"/>
        <property type="gene ID" value="ENSBIXG00005019701.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161018"/>
    <dbReference type="OMA" id="QHAEPDG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003180">
        <property type="term" value="P:aortic valve morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF197">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13 17 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611592" evidence="20">
        <location>
            <begin position="20"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="21">
        <location>
            <begin position="321"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="49"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 18">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="16">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
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    <sequence length="1225" mass="135218" checksum="B2B7FFA27C241D6D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="8 9 10" key="1">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <ligand>
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</entry>
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    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="320" mass="36056" checksum="8E0A7CB19D93A759" modified="2019-09-18" version="1">MTNMSLVTTFVLTGLPHAPELDTFLFGIFLVIYVLTVVGNFLILLVIIVNPHLHTPMYYFLTNLSFIDMWYSTVTVPKMLMTLVSPEGSPISFHSCVAQFYSFDFLGSTECFLYTVMSYDRFLAISYPLRYASMMSGRTCAILATTTWLSGSLHSAAQTTPTFRLPFCGPNQIQHYICDAPSVLKLACADTSTVEMVIFVNIGVVALGCFLLIVLSYVSIVHSILKIRTSEGRWRAFQTCASHCIVVLCFYVPLVFIYLRPGSKEVVDRIVAVFYTVMTPLLNPVVYTLRNKDVKKALLKLKDKVMYSQSKWSLQCRLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GV87</accession>
    <name>A0A4W2GV87_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Olfactory receptor 10G9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113886050</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027387788.1">
        <property type="nucleotide sequence ID" value="XM_027531987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GV87"/>
    <dbReference type="STRING" id="30522.A0A4W2GV87"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011868.1">
        <property type="protein sequence ID" value="ENSBIXP00000027929.1"/>
        <property type="gene ID" value="ENSBIXG00000000200.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035631.1">
        <property type="protein sequence ID" value="ENSBIXP00005021669.1"/>
        <property type="gene ID" value="ENSBIXG00005024622.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886050"/>
    <dbReference type="GeneTree" id="ENSGT01050000244869"/>
    <dbReference type="OMA" id="CASHCIM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="310" mass="34724" checksum="484B0FD938DF39E6" modified="2019-09-18" version="1">MTNMSLVTTFILMGLPHAPELDTLLFGIFLVIYVLTVVGNLLILLVIIVNPHLHTPMYYFLTNLSFIDMWFSTVTVPKMLMTLVSPEGSPVSFPSCVAQLYSFHFLGSTECFLYTVMSYDRFLAISYPLRYASMMSGRTCAILATTTWLSGSVHSAAQTTLTFRLPFCGPNQIQHYFCDAPPILKLACADTSTMEMVIFVNIGVVASGCFLLIVLSYMSIIHSILKIHTSEGRWRAFQTCASHCTVVLCFFVPCVFIYLRPGSKEAMDSIVAVFYTVMTPLLNPVVYTLRNKEVKKALLKLKDKVVYLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GXE4</accession>
    <name>A0A4W2GXE4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ring finger and CHY zinc finger domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RCHY1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GXE4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050361.1">
        <property type="protein sequence ID" value="ENSBIXP00000040962.1"/>
        <property type="gene ID" value="ENSBIXG00000025149.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036327.1">
        <property type="protein sequence ID" value="ENSBIXP00005022140.1"/>
        <property type="gene ID" value="ENSBIXG00005025176.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008853"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000009">
        <property type="entry name" value="RING finger and CHY zinc finger domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF53">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="9">
        <location>
            <begin position="13"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="10">
        <location>
            <begin position="82"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005022140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="252" mass="29055" checksum="E2F9A8E3EF826EA3" modified="2019-09-18" version="1">MAFSALEDGARGQAQRRRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCSVCHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGKHKCIENVSRQNCPICLEDIHTSRIAAQVLPCGHLLHRGYRCPLCMRSALDMSRSWRQRDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCNICESYNTAQAGKYRISIDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H3C7</accession>
    <name>A0A4W2H3C7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="22">SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SMARCA4</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027403503.1">
        <property type="nucleotide sequence ID" value="XM_027547702.1"/>
    </dbReference>
    <dbReference type="STRING" id="30522.A0A4W2H3C7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043171.1">
        <property type="protein sequence ID" value="ENSBIXP00000044025.1"/>
        <property type="gene ID" value="ENSBIXG00000028249.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041350.1">
        <property type="protein sequence ID" value="ENSBIXP00005025560.1"/>
        <property type="gene ID" value="ENSBIXG00005028150.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895895"/>
    <dbReference type="CTD" id="6597"/>
    <dbReference type="GeneTree" id="ENSGT00940000156887"/>
    <dbReference type="OMA" id="YGPGHKL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071564">
        <property type="term" value="C:npBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050681">
        <property type="term" value="F:nuclear androgen receptor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:p53 binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030957">
        <property type="term" value="F:Tat protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043923">
        <property type="term" value="P:host-mediated activation of viral transcription"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060766">
        <property type="term" value="P:negative regulation of androgen receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003407">
        <property type="term" value="P:neural retina development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902661">
        <property type="term" value="P:positive regulation of glucose mediated signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902895">
        <property type="term" value="P:positive regulation of miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901798">
        <property type="term" value="P:positive regulation of signal transduction by p53 class mediator"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901838">
        <property type="term" value="P:positive regulation of transcription of nucleolar large rRNA by RNA polymerase I"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05516">
        <property type="entry name" value="Bromo_SNF2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18062">
        <property type="entry name" value="DEXHc_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000004">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.120:FF:000001">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000116">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000089">
        <property type="entry name" value="Putative global transcription activator SNF2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030100">
        <property type="entry name" value="BRG1_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08880">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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        </source>
    </evidence>
    <sequence length="248" mass="26294" checksum="CF4CDC294437F28C" modified="2019-09-18" version="1">MFLFSSLPVLLCLVTVSFSNTKAVEDAQKTCPVVACAIPVTNGTPGRDGRDGPKGEKGEPGQGLRGSQGPPGKMGPPGNIGNPGLPGPRGYKGDRGDSSVAEAKLASLERQIRDLRSELDHVKKLQTFSLGKKSGKKLYVTNREKMPFSSVKALCTALGATVATPKNAEENKAIQDMASDTAFLGITDEVTEGQFMYVTGGRLGYSNWKKNEPNNYGSGEDCVSLLPDGLWNDISCSSSFLAICEFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H8C7</accession>
    <name>A0A4W2H8C7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbonic anhydrase</fullName>
            <ecNumber evidence="11">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CA2</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>urea = cyanamide + H2O</text>
            <dbReference type="Rhea" id="RHEA:23056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:16698"/>
            <dbReference type="EC" id="4.2.1.69"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027417426.1">
        <property type="nucleotide sequence ID" value="XM_027561625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H8C7"/>
    <dbReference type="SMR" id="A0A4W2H8C7"/>
    <dbReference type="STRING" id="30522.A0A4W2H8C7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018536.1">
        <property type="protein sequence ID" value="ENSBIXP00000009973.1"/>
        <property type="gene ID" value="ENSBIXG00000015570.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042764.1">
        <property type="protein sequence ID" value="ENSBIXP00005026492.1"/>
        <property type="gene ID" value="ENSBIXG00005004993.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904451"/>
    <dbReference type="CTD" id="760"/>
    <dbReference type="GeneTree" id="ENSGT00940000160385"/>
    <dbReference type="OMA" id="INPHWKV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018820">
        <property type="term" value="F:cyanamide hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF120">
        <property type="entry name" value="CARBONIC ANHYDRASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="12">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="260" mass="29114" checksum="A89412C23FFD19A7" modified="2019-09-18" version="1">MSHHWGYGKHNGPEHWHKDFPIANGERQSPVDIDTKAVVQDPALKPLALVYGEATSRRMVNNGHSFNVEYDDSQDKAVLKDGPLTGTYRLVQFHFHWGSSDDQGSEHTVDRKKYAAELHLVHWNTKYGDFGTAAQQPDGLAVVGVFLKVGDANPALQKVLDALDSIKTKGKSTDFPNFDPGSLLPNVLDYWTYPGSLTTPPLLESVTWIVLKEPISVSSQQMLKFRTLNFNAEGEPELLMLANWRPAQPLKNRQVRGFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HME1</accession>
    <name>A0A4W2HME1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myosin light chain kinase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MYLK3</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027369506.1">
        <property type="nucleotide sequence ID" value="XM_027513705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HME1"/>
    <dbReference type="SMR" id="A0A4W2HME1"/>
    <dbReference type="STRING" id="30522.A0A4W2HME1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016551.1">
        <property type="protein sequence ID" value="ENSBIXP00000008737.1"/>
        <property type="gene ID" value="ENSBIXG00000000947.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005002258.1">
        <property type="protein sequence ID" value="ENSBIXP00005032257.1"/>
        <property type="gene ID" value="ENSBIXG00005012755.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875386"/>
    <dbReference type="CTD" id="91807"/>
    <dbReference type="GeneTree" id="ENSGT00940000160007"/>
    <dbReference type="OMA" id="IHVQEMD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004687">
        <property type="term" value="F:myosin light chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071347">
        <property type="term" value="P:cellular response to interleukin-1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905710">
        <property type="term" value="P:positive regulation of membrane permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905075">
        <property type="term" value="P:positive regulation of tight junction disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14192">
        <property type="entry name" value="STKc_MLCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000196">
        <property type="entry name" value="Myosin light chain kinase family, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000135">
        <property type="entry name" value="Putative myosin light chain kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF20">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="482"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005032257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="786" mass="85238" checksum="1C33F6E33BCC58F5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HWT1</accession>
    <name>A0A4W2HWT1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HIPK4</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_027371613.1">
        <property type="nucleotide sequence ID" value="XM_027515812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HWT1"/>
    <dbReference type="STRING" id="30522.A0A4W2HWT1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020740.1">
        <property type="protein sequence ID" value="ENSBIXP00000011415.1"/>
        <property type="gene ID" value="ENSBIXG00000016650.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019451.1">
        <property type="protein sequence ID" value="ENSBIXP00005034458.1"/>
        <property type="gene ID" value="ENSBIXG00005015421.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876504"/>
    <dbReference type="CTD" id="147746"/>
    <dbReference type="GeneTree" id="ENSGT00940000161512"/>
    <dbReference type="OMA" id="DMTMDAE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035173">
        <property type="term" value="F:histone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901796">
        <property type="term" value="P:regulation of signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000029">
        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF46">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="527"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="616" mass="69316" checksum="2E817B0BE22119DF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYG5</accession>
    <name>A0A4W2HYG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox protein goosecoid</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GSC</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377475.1">
        <property type="nucleotide sequence ID" value="XM_027521674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HYG5"/>
    <dbReference type="SMR" id="A0A4W2HYG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043691.1">
        <property type="protein sequence ID" value="ENSBIXP00000008581.1"/>
        <property type="gene ID" value="ENSBIXG00000014379.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046897.1">
        <property type="protein sequence ID" value="ENSBIXP00005036419.1"/>
        <property type="gene ID" value="ENSBIXG00005006390.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879849"/>
    <dbReference type="CTD" id="145258"/>
    <dbReference type="GeneTree" id="ENSGT00940000160635"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021904">
        <property type="term" value="P:dorsal/ventral neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042474">
        <property type="term" value="P:middle ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048644">
        <property type="term" value="P:muscle organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014036">
        <property type="term" value="P:neural crest cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023019">
        <property type="term" value="P:signal transduction involved in regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000210">
        <property type="entry name" value="homeobox protein goosecoid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="161"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="214"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005036419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="257" mass="28045" checksum="41F2F554161E0019" modified="2019-09-18" version="1">MPASMFSIDNILATRPRCKDSVLPVAPSAATPVVFPALHGDSLYGGAGGGASSDYGAFYPRPVAPGGAGLPAAVGGSRLGYSNYFYGQLHVQAAPVGPACCGAVPPLGAQQCSCVPTPPGYEGPGSVLVSAVPHQMMPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEESENAEKWNKTSSKASPEKREEEGKSDLDSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I770</accession>
    <name>A0A4W2I770_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATP1B2</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375185.1">
        <property type="nucleotide sequence ID" value="XM_027519384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I770"/>
    <dbReference type="SMR" id="A0A4W2I770"/>
    <dbReference type="STRING" id="30522.A0A4W2I770"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015837.1">
        <property type="protein sequence ID" value="ENSBIXP00000008251.1"/>
        <property type="gene ID" value="ENSBIXG00000014132.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031333.1">
        <property type="protein sequence ID" value="ENSBIXP00005039683.1"/>
        <property type="gene ID" value="ENSBIXG00005022109.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878371"/>
    <dbReference type="CTD" id="482"/>
    <dbReference type="GeneTree" id="ENSGT01030000234579"/>
    <dbReference type="OMA" id="DHIPTYQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000068">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1660:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01107">
        <property type="entry name" value="Na_K_ATPase_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF26">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00390">
        <property type="entry name" value="ATPASE_NA_K_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00391">
        <property type="entry name" value="ATPASE_NA_K_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="290" mass="33406" checksum="CC453C76E2AAB296" modified="2019-09-18" version="1">MVIQKEKKSCGQVVEEWKEFVWNPRTHQFMGRTGTSWAFILLFYLVFYGFLTAMFTLTMWVMLQTVSDHTPKYQDRLATPGLMIRPKTENLDVIVNVSDTESWDQHVQKLNKFLEPYNDSIQAQKNDVCRPGRYYEQPDNGVLNYPKRACQFNRTQLGDCSGIGDPTHYGYSTGQPCVFIKMNRVINFYAGANQSMNVTCVGKRDEDAENLGNFVMFPANGNIDLMYFPYYGKKFHVNYTQPLVAVKFLNVTPNVEVNVECRINAANIATDDERDKFAGRVAFKLRINKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IEW3</accession>
    <name>A0A4W2IEW3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Noggin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NOG</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Interacts with GDF5; inhibits chondrocyte differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the noggin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027374824.1">
        <property type="nucleotide sequence ID" value="XM_027519023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IEW3"/>
    <dbReference type="SMR" id="A0A4W2IEW3"/>
    <dbReference type="STRING" id="30522.A0A4W2IEW3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009953.1">
        <property type="protein sequence ID" value="ENSBIXP00000028552.1"/>
        <property type="gene ID" value="ENSBIXG00000009794.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005040658.1">
        <property type="protein sequence ID" value="ENSBIXP00005043859.1"/>
        <property type="gene ID" value="ENSBIXG00005027624.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878142"/>
    <dbReference type="CTD" id="9241"/>
    <dbReference type="GeneTree" id="ENSGT00390000006009"/>
    <dbReference type="OMA" id="LWSHTFC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSBIXG00000029533.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006059.1">
        <property type="protein sequence ID" value="ENSBIXP00005031015.1"/>
        <property type="gene ID" value="ENSBIXG00005001056.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161811"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000010">
        <property type="entry name" value="Myosin regulatory light chain 2, atrial isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000007">
        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="5" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="168" mass="19156" checksum="7196AE6C4AB3FF59" modified="2019-09-18" version="1">MAPRRARKKAEGGASSNVFSMFDQSQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRINVKNEELEAMVKEAPGPINFTVFLTMFGEKLKGTDPEETILHAFKVFDTEGKGFVKADFIKEKLMTQADRFSEEEVKQMFAAFPPDVCGNLDYRNLCYVITHGEEKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMH7</accession>
    <name>A0A4W2BMH7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Sex-determining region Y protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Involved in different aspects of gene regulation including promoter activation or repression. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending. Also involved in pre-mRNA splicing. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CALM, EP300, HDAC3, KPNB1, ZNF208 isoform KRAB-O, PARP1, SLC9A3R2 and WT1. The interaction with EP300 modulates its DNA-binding activity. The interaction with KPNB1 is sensitive to dissociation by Ran in the GTP-bound form. Interaction with PARP1 impaired its DNA-binding activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the SRY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMH7"/>
    <dbReference type="STRING" id="30522.A0A4W2BMH7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053590.1">
        <property type="protein sequence ID" value="ENSBIXP00000000206.1"/>
        <property type="gene ID" value="ENSBIXG00000030119.1"/>
    </dbReference>
    <dbReference type="OMA" id="DCTKATH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030238">
        <property type="term" value="P:male sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22028">
        <property type="entry name" value="HMG-box_SoxA_SoxB_SoxG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017253">
        <property type="entry name" value="SRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF161">
        <property type="entry name" value="SEX-DETERMINING REGION Y PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037653">
        <property type="entry name" value="SRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 18" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 18" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12 18 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15 18 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 18" id="KW-0726">Sexual differentiation</keyword>
    <keyword evidence="14 18" id="KW-0804">Transcription</keyword>
    <keyword evidence="11 18" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="21">
        <location>
            <begin position="61"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="19">
        <location>
            <begin position="61"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR037653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="205" mass="23877" checksum="8902D5F48908CAF9" modified="2019-09-18" version="1">VQSYASAMFRVLNDDVYSPAVVQQQTTLAFRKDSSLCTDSHSANDQCERGEHVRESSQDHVKRPMNAFIVWSRERRRKVALENPKMKNSDISKQLGYEWKRLTDAEKRPFFEEAQRLLAIHRDKYPGYKYRPRRRAKRPQKSLPADSSILCNPMHVETLHPFTYRDGCAKTTYSQMESQLSRSQSVIITNSLLQKEHHSSWTSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BP24</accession>
    <name>A0A4W2BP24_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BP24"/>
    <dbReference type="STRING" id="30522.A0A4W2BP24"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013302.1">
        <property type="protein sequence ID" value="ENSBIXP00000001755.1"/>
        <property type="gene ID" value="ENSBIXG00000008294.1"/>
    </dbReference>
    <dbReference type="OMA" id="HDWFESR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000203">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="20"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="369" mass="41269" checksum="856339414A3F026B" modified="2019-09-18" version="1">MPGAVERPSWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKMVAIKCIAKKALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPSSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASRGELLAPAAGGPVAGCCCRDCCVEPSPKLSPSLHPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQ77</accession>
    <name>A0A4W2BQ77_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">ATPase WRNIP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Werner helicase-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Functions as a modulator of initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. In the presence of ATP, stimulation of DNA polymerase delta-mediated DNA synthesis is decreased. Also plays a role in the innate immune defense against viruses. Stabilizes the RIGI dsRNA interaction and promotes RIGI 'Lys-63'-linked polyubiquitination. In turn, RIGI transmits the signal through mitochondrial MAVS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="23">Forms homooligomers, possibly octamers. Directly interacts with POLD1, POLD2 and POLD4. Interacts with the N-terminal domain of WRN. Interacts (via UBZ4-type zinc finger) with monoubiquitin and polyubiquitin. Interacts with TRIM14 and PPP6C; these interactions positively regulate the RIGI signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQ77"/>
    <dbReference type="SMR" id="A0A4W2BQ77"/>
    <dbReference type="STRING" id="30522.A0A4W2BQ77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012472.1">
        <property type="protein sequence ID" value="ENSBIXP00000002166.1"/>
        <property type="gene ID" value="ENSBIXG00000008655.1"/>
    </dbReference>
    <dbReference type="OMA" id="RIILSQC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3710.10:FF:000002">
        <property type="entry name" value="ATPase WRNIP1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000054">
        <property type="entry name" value="ATPase WRNIP1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000331">
        <property type="entry name" value="E3 ubiquitin-protein ligase RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000001">
        <property type="entry name" value="Putative AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000137">
        <property type="entry name" value="Replication-associated recombination protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3710.10">
        <property type="entry name" value="DNA polymerase III clamp loader subunits, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040539">
        <property type="entry name" value="Znf-WRNIP1_ubi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18279">
        <property type="entry name" value="zf-WRNIP1_ubi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51908">
        <property type="entry name" value="ZF_UBZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 26" id="KW-0227">DNA damage</keyword>
    <keyword evidence="19 26" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="49"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="126"/>
            <end position="146"/>
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        <location>
            <begin position="147"/>
            <end position="162"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
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    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00056760"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00066151"/>
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    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00068616"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00080168"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01256"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="638" mass="68398" checksum="3A553F3341092D78" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BRU3</accession>
    <name>A0A4W2BRU3_BOBOX</name>
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        <submittedName>
            <fullName evidence="17">Zinc finger protein 197</fullName>
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    </protein>
    <gene>
        <name evidence="18" type="primary">ZNF197</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSBIXP00005043196.1"/>
        <property type="gene ID" value="ENSBIXG00005026836.1"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SCAN"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 19"/>
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    </dbReference>
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        <property type="entry name" value="Zinc finger protein 197"/>
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        <property type="entry name" value="zinc finger protein 197"/>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
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    </dbReference>
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        <property type="entry name" value="C2H2-ZF_domain"/>
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        <property type="entry name" value="KRAB"/>
    </dbReference>
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        <property type="entry name" value="KRAB_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF242">
        <property type="entry name" value="ZINC FINGER PROTEIN 628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00349">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="15">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="16">
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        </location>
    </feature>
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            <begin position="958"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001895.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1029" mass="119482" checksum="C7213E9A91E892D5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTV7</accession>
    <name>A0A4W2BTV7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTV7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009700.1">
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    </dbReference>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
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        <property type="entry name" value="RT_POL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="158"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="495" mass="57112" checksum="8877248881E5F2DA" modified="2019-09-18" version="1">MVCMKNNLLFYLLCKIGHDQARRDKKAFFSDQCKEIEENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDSDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKTSVFIPIPKKGNAKEGSNYRTTALISHASKVMLKILQARLQQYMNRELPDVQAGFRKGRGTREQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYMMRNAGLEETQTGIEIARRNINNLRYADDTTLMAESEEEPKSLLMKVKVESEKVGLKLNIQKTKIMASGPITFWEIDGETVETVSDFIFLGSKITTDGDCTHEIKRRLLLGRKVMTNLDSIKSRHIILPTKVHLVKAIVLPVVMYGCESWTVKKAERRRIDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTZ3</accession>
    <name>A0A4W2BTZ3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cathelicidin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTZ3"/>
    <dbReference type="STRING" id="30522.A0A4W2BTZ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009526.1">
        <property type="protein sequence ID" value="ENSBIXP00000003593.1"/>
        <property type="gene ID" value="ENSBIXG00000010017.1"/>
    </dbReference>
    <dbReference type="OMA" id="MMETTCA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000003">
        <property type="entry name" value="Cathelicidin antimicrobial peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001894">
        <property type="entry name" value="Cathelicidin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018216">
        <property type="entry name" value="Cathelicidin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10206">
        <property type="entry name" value="CATHELICIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10206:SF2">
        <property type="entry name" value="CATHELICIDIN ANTIMICROBIAL PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00666">
        <property type="entry name" value="Cathelicidins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00947">
        <property type="entry name" value="CATHELICIDINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021447266" evidence="9">
        <location>
            <begin position="30"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="159" mass="17405" checksum="92AAFD47261FCFCD" modified="2019-09-18" version="1">METQRASLSLGRWSLWLLLLGLVLPSASAQALSYREAMLRAVDQLNERVLRSSSLPPPGVEDLGARKPVNFRVKETVCPRSNLQPPEQCDFKENGVSLGTETEGGLWNMLRTDDPLPHPGQRKALLPGPLPPPSPRSPALALHPLEQCFCNAVPTPELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVG5</accession>
    <name>A0A4W2BVG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Asparagine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NARS1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVG5"/>
    <dbReference type="SMR" id="A0A4W2BVG5"/>
    <dbReference type="STRING" id="30522.A0A4W2BVG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008289.1">
        <property type="protein sequence ID" value="ENSBIXP00000004213.1"/>
        <property type="gene ID" value="ENSBIXG00000010578.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033553.1">
        <property type="protein sequence ID" value="ENSBIXP00005020263.1"/>
        <property type="gene ID" value="ENSBIXG00005023373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
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        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
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        <property type="entry name" value="Asn-tRNA-ligase"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="NA-bd_OB_tRNA"/>
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        <property type="entry name" value="asnS"/>
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        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
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        <property type="entry name" value="tRNA-synt_2"/>
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        <property type="entry name" value="tRNA_anti-codon"/>
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        <property type="entry name" value="TRNASYNTHASP"/>
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    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        <location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039867"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004213.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="559" mass="64431" checksum="61B2147DCC43575C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C182</accession>
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        <submittedName>
            <fullName evidence="13">Transcription factor 7 like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TCF7L2</name>
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    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000000909.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021438.1">
        <property type="protein sequence ID" value="ENSBIXP00005012203.1"/>
        <property type="gene ID" value="ENSBIXG00005016649.1"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HMG-box_TCF7-like"/>
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    </dbReference>
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        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="transcription factor 7-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Catenin-bd_sf"/>
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        <property type="entry name" value="CTNNB1-bd_N"/>
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        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF32">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="12">
        <location>
            <begin position="327"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="327"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="459" mass="51171" checksum="DAD14DCD2169A5D8" modified="2019-09-18" version="1">MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGTLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSEVGSLHSAKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSECFLNPCLSLPPITDLSAPKKCRARFGLDQQNNWCGPCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1T0</accession>
    <name>A0A4W2C1T0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C1T0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053543.1">
        <property type="protein sequence ID" value="ENSBIXP00000000227.1"/>
        <property type="gene ID" value="ENSBIXG00000030082.1"/>
    </dbReference>
    <dbReference type="OMA" id="CMNENGK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
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        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="185"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="612" mass="70880" checksum="5AB99FFE99BA9F84" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C218</accession>
    <name>A0A4W2C218_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tripartite motif containing 80</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C218"/>
    <dbReference type="SMR" id="A0A4W2C218"/>
    <dbReference type="STRING" id="30522.A0A4W2C218"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003193.1">
        <property type="protein sequence ID" value="ENSBIXP00000006725.1"/>
        <property type="gene ID" value="ENSBIXG00000012805.1"/>
    </dbReference>
    <dbReference type="OMA" id="GFIPKVT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19802">
        <property type="entry name" value="Bbox1_TRIM8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13737">
        <property type="entry name" value="SPRY_PRY_TRIM25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF34">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="73"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="208"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="426"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="70507" checksum="5F8EF36605E3970E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C557</accession>
    <name>A0A4W2C557_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit 6-interacting protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit E-interacting protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">EIF3L</name>
        <name evidence="4" type="synonym">EIF3EIP</name>
        <name evidence="4" type="synonym">EIF3S6IP</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RRN3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027396632.1">
        <property type="nucleotide sequence ID" value="XM_027540831.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00000006702.1"/>
        <property type="gene ID" value="ENSBIXG00000006883.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038581.1">
        <property type="protein sequence ID" value="ENSBIXP00005042806.1"/>
        <property type="gene ID" value="ENSBIXG00005026466.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892243"/>
    <dbReference type="CTD" id="51386"/>
    <dbReference type="GeneTree" id="ENSGT00390000000411"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
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    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="eIF3l"/>
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        <property type="entry name" value="PCI_dom"/>
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
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        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="233"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="466" mass="55194" checksum="1CE2ABA807F91DA7" modified="2019-09-18" version="1">MSYRPVVSPAMSLTRRCMRFRTSMRTDAVFLILYKELYYRHIYAKVSGGPSLEQRFESYYNYCNLFNYILNADGPAPLELPNQWLWDIIDEFIYQFQSFSQYRCKTAKKSEEEIDFLRSNPKIWNVHSVLNVLHSLVDKSNINRQLEVYTSGGDPESVAGEYGRHSLYKMLGYFSLVGLLRLHSLLGDYYQAIKVLENIELNKKSMYSRVPECQVTTYYYVGFAYLMMRRYQDAIRVFANILLYIQRTKSMFQRTTYKYEMINKQNEQMHALLAIALTMYPMRIDESIHLQLREKYGDKMLRMQKGDPQVYEELFSYSCPKFLSPVVPNYDNVHPNYHKEPFLQQLKVFSDEVQQQAQLSTIRSFLKLYTTMPVAKLAGFLDLTEQEFRIQLLVFKHKMKNLVWTSGISALDGEFQSASEVDFYIDKDMIHIADTKVARRYGDFFIRQIHKFEELNRTLKKMGQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CA47</accession>
    <name>A0A4W2CA47_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CA47"/>
    <dbReference type="STRING" id="30522.A0A4W2CA47"/>
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        <property type="protein sequence ID" value="ENSBIXP00000009754.1"/>
        <property type="gene ID" value="ENSBIXG00000002218.1"/>
    </dbReference>
    <dbReference type="OMA" id="FAIHRYI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="57"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="233" mass="25830" checksum="4AC12C1C44EF3CED" modified="2019-09-18" version="1">MADKKGKPGEAEAPPPQKGEKDPEEPKKKSEQPKDEVGTRKGCRRYKWEFKDSNKEFWSVGHAEVKLISLACLIGSLVMFTGSTVHPLLTLIITMEISIFIFFFIIYTFAIQRYLPFILWPISDLLNDLFCCGFLVGAAVFALRSRQTVPTNYLAAVVFLCVAALFALVDVCLQKKHFKGKKIKRNVLVPPPPKEPEKPVAEVKPVDAEKKKEAGKPKEAKAKGAAGAKGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAW3</accession>
    <name>A0A4W2CAW3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carbonic anhydrase</fullName>
            <ecNumber evidence="13">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CA3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 13">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027417428.1">
        <property type="nucleotide sequence ID" value="XM_027561627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAW3"/>
    <dbReference type="SMR" id="A0A4W2CAW3"/>
    <dbReference type="STRING" id="30522.A0A4W2CAW3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018553.1">
        <property type="protein sequence ID" value="ENSBIXP00000009979.1"/>
        <property type="gene ID" value="ENSBIXG00000007345.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042706.1">
        <property type="protein sequence ID" value="ENSBIXP00005044916.1"/>
        <property type="gene ID" value="ENSBIXG00005028779.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904452"/>
    <dbReference type="CTD" id="761"/>
    <dbReference type="GeneTree" id="ENSGT00940000159435"/>
    <dbReference type="OMA" id="NYPMAKG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03119">
        <property type="entry name" value="alpha_CA_I_II_III_XIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF127">
        <property type="entry name" value="CARBONIC ANHYDRASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="14">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="260" mass="29370" checksum="F78A6E7E9C47028B" modified="2019-09-18" version="1">MAKEWGYADHNGPDHWHELFPNAKGENQSPIELNTKEISHDPSLKPWTASYDPGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLAAPYRLRQFHLHWGSSDDHGSEHSVDGVKYAAELHLVHWNSKYNSYATALKHADGIAVVGVFLKIGREKGEFQLLLDALDKIKTKGKEAPFNNFNPSCLFPACRDYWTYHGSFTTPPCEECIVWLLLKEPITVSSDQIAKLRTLYSSAENEPPVPLVRNWRPPQPIKGRIVKASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CD31</accession>
    <name>A0A4W2CD31_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CD31"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008193.1">
        <property type="protein sequence ID" value="ENSBIXP00000004265.1"/>
        <property type="gene ID" value="ENSBIXG00000010622.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="435" mass="49753" checksum="950B015BE07A01C5" modified="2019-09-18" version="1">MGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSKYHTIALISHASKVMLKILQARLQQYVIRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKSIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYADYIMRNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSPLKEMSEKVGLKLNIQKTKIMASGPITSWEIDGETVETVSDFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSIFKSRDITLPTKVCLVKAMVFPVVMYGCESWTVKKAECQRIDAFELWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CDD2</accession>
    <name>A0A4W2CDD2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 18">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">GPI</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="5 18"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CDD2"/>
    <dbReference type="STRING" id="30522.A0A4W2CDD2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019972.1">
        <property type="protein sequence ID" value="ENSBIXP00000010899.1"/>
        <property type="gene ID" value="ENSBIXG00000016297.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043692.1">
        <property type="protein sequence ID" value="ENSBIXP00005027048.1"/>
        <property type="gene ID" value="ENSBIXG00005029128.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000707"/>
    <dbReference type="OMA" id="DWYRQLW"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034101">
        <property type="term" value="P:erythrocyte homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904539">
        <property type="term" value="P:negative regulation of glycolytic process through fructose-6-phosphate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010595">
        <property type="term" value="P:positive regulation of endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002639">
        <property type="term" value="P:positive regulation of immunoglobulin production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000093">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 18" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="14 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="15 18" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="565" mass="63636" checksum="FA5D5D10DFD7793B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CEX4</accession>
    <name>A0A4W2CEX4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acid sphingomyelinase-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SMPDL3A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cyclic-nucleotide phosphodiesterase that acts as a negative regulator of innate immunity by mediating degradation of 2',3'-cGAMP, thereby inhibiting the cGAS-STING signaling. Specifically linearizes 2',3'-cGAMP into 2'5'-bond pGpA and further hydrolyzes pGpA to produce GpA. Also has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP. Has in vitro activity with p-nitrophenyl-TMP. Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates. Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine. Does not have sphingomyelin phosphodiesterase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2',3'-cGAMP + H2O = 5'-pGpA(2'-5') + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78339"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:143093"/>
            <dbReference type="ChEBI" id="CHEBI:228270"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:78340"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5'-pGpA(2'-5') + H2O = 5'-GpA(2'-5') + phosphate</text>
            <dbReference type="Rhea" id="RHEA:78343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:228270"/>
            <dbReference type="ChEBI" id="CHEBI:228271"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:78344"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:23681"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Homodimer; homodimerizes following 2',3'-cGAMP-binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the acid sphingomyelinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027406890.1">
        <property type="nucleotide sequence ID" value="XM_027551089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CEX4"/>
    <dbReference type="SMR" id="A0A4W2CEX4"/>
    <dbReference type="STRING" id="30522.A0A4W2CEX4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018911.1">
        <property type="protein sequence ID" value="ENSBIXP00000010215.1"/>
        <property type="gene ID" value="ENSBIXG00000007079.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898167"/>
    <dbReference type="CTD" id="10924"/>
    <dbReference type="OMA" id="TAWHCRS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160049">
        <property type="term" value="P:negative regulation of cGAS/STING signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000044">
        <property type="entry name" value="acid sphingomyelinase-like phosphodiesterase 3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017064">
        <property type="entry name" value="ASM-like_Pdiesterase_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045473">
        <property type="entry name" value="ASM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF24">
        <property type="entry name" value="ACID SPHINGOMYELINASE-LIKE PHOSPHODIESTERASE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19272">
        <property type="entry name" value="ASMase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036767">
        <property type="entry name" value="ASM-like_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16 17" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021523235" description="Acid sphingomyelinase-like phosphodiesterase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="18">
        <location>
            <begin position="37"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin phosphodiesterase C-terminal" evidence="19">
        <location>
            <begin position="305"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR036767-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF19272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="450" mass="51233" checksum="B5F813D8C233EBEB" modified="2019-09-18" version="1">MARLGALVCCLLAAWHCRPGLGLPLAPAGTGPAVGQFWHVTDFHLDPTYHITGDHTKVCASSKGAEASDPGPFGDVMCDSPYRLIFSALDFIKNSGQKVSFMIWTGDSPPHVPVLELSTDKVINVIANITTTIQRLFPNLQVFPALGNHDYWPQDQLPVVNSKVYNAVANLWKPWLTEDAITTLRKGGFYTQKVSNNPKLRIISLNTNLYYGPNSVTLNQTDPANQFEWLENTLNISQQNKEKVYIIAHVPVGYLPYARGISAMRKYHNEKLIDIFRKYSDIIAGQFYGHTHRDSIMVLSDKKGKPVNSLFVAPAVTPVRSVLERLTNNPGVRLFQYDPRDYKLLDMLQYYLNLTDANLKGESNWKLEYNLTQAYDIQDLQPKSLYKLAKQFAIQESKQFIKYYKYFFVSYDSSVICQGKCKIFQICAIMNLDVISYTDCFRQYHMKHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFH3</accession>
    <name>A0A4W2CFH3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho guanine nucleotide exchange factor 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARHGEF17</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPases.</text>
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    <dbReference type="STRING" id="30522.A0A4W2CFH3"/>
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        <property type="protein sequence ID" value="ENSBIXP00000011770.1"/>
        <property type="gene ID" value="ENSBIXG00000016978.1"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 17"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 17"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="ARHGEF10/ARHGEF17"/>
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        <property type="entry name" value="DBL_dom_sf"/>
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        <property type="entry name" value="DH_dom"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
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    </dbReference>
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        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 17"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40_2"/>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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        <property type="entry name" value="DH_2"/>
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    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
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            <end position="1254"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011770.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2061" mass="220651" checksum="A2DD0FCAE2485B9C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHL8</accession>
    <name>A0A4W2CHL8_BOBOX</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <sequence length="365" mass="41746" checksum="48FC9519AB611A1E" modified="2019-09-18" version="1">MLLSLVLHMHSVRCVLPAAVLLGTAPTYVLVWGAWRLLSALLPSRFYQAVDDRLYCVYQSMVLFFFENYTGVQILLYGDLPKNKENIIYLANHQSTVDWIIADILAVRQSALGHVRYVLKDGLKWLPLYGCYFSQHGGIYVKRSAKFNENQMRKKLQRYINAGTPMYLVIFPEGTRYNPELTKVISASQAFAAQEGLPVLKHVLTPRIKATHVAFDSMKDYLDAVYDVTVAFEGSVDDKGQRKEAPSMAEFLCKECPKIHIHIDRIDKKNIPEEQVSMKRWLHERFEIKDKLLIEFYDSPDPERRNKFPGESVNSKLSLKKTLPSFLILSGLTAGLLMTEAGRKLYVKTWIYGTLIGCLWVSIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI05</accession>
    <name>A0A4W2CI05_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CI05"/>
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        <property type="protein sequence ID" value="ENSBIXP00000012677.1"/>
        <property type="gene ID" value="ENSBIXG00000017739.1"/>
    </dbReference>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SMART" id="SM00409"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012677.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="220" mass="24500" checksum="E9A686253ACC250A" modified="2019-09-18" version="1">MDREKQITSRKTNTLSITEMTFDKTGLYHCSYQRGGRWSQFSDPLQLVMTGAYSKPSLSSMAGTVVAPGETVKLQCVSKINYDIFILTKEDGDHVTQNQSSVPHDGGRQTIFLLNPVSSTQAGTYRCYGAFHEYPYVWSQPSDPLQLQVEGTTDSPSITQPRHSTAPRAWHPSVETQVRLSLAALLLLVMVVLLVEAWCSRGGPSVKSDEPPPQWTDKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CIY7</accession>
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            <taxon>Eutheria</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="791" mass="87816" checksum="82F340A607E4BADC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKY2</accession>
    <name>A0A4W2CKY2_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Ras and Rab interactor 2</fullName>
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    </protein>
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        <name evidence="8" type="primary">RIN2</name>
    </gene>
    <organism evidence="8 9">
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIN (Ras interaction/interference) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CKY2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024415.1">
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        <property type="gene ID" value="ENSBIXG00000018645.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005008810.1">
        <property type="protein sequence ID" value="ENSBIXP00005004193.1"/>
        <property type="gene ID" value="ENSBIXG00005010179.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154866"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ras and Rab interactor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vps9-like"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF51">
        <property type="entry name" value="RAS AND RAB INTERACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="VPS9" evidence="7">
        <location>
            <begin position="185"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="6">
        <location>
            <begin position="354"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="462" mass="52978" checksum="73C4EFAA24547FC2" modified="2019-09-18" version="1">MSAQTMGTRGLDKRGSFFKLIDTIASEIGELKQEMVQTDVSLENGLGPTEAHSMVRHKDDHYSEETDAKTCPRDSGYDSLSNRLSILDRLLHTHPIWLQLSLSEEEAAGVLQAQPPGIFLVRKSTKMQKKVLCLRLPCEFGVPLKEFAIKESTYNVVLEKAMHKCILKPLKGHIEAMLKHFHVADGSWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKMYSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEVEYMMELLDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSEARDTLRQWHKRRTTTRTVPSVDDFQNYLRVAFQEVNSGCTGKTLLVRPYVTTEDVCQLCAEKFKVAEPQEYSLFLFVDETWQQLAEDTYPQKIKAELHSRPQPHVFHFVYKRIKKDPYGIIFQNGEEDLSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMG5</accession>
    <name>A0A4W2CMG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CIDE-N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CMG5"/>
    <dbReference type="STRING" id="30522.A0A4W2CMG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000023167.1">
        <property type="protein sequence ID" value="ENSBIXP00000012971.1"/>
        <property type="gene ID" value="ENSBIXG00000017973.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015121">
        <property type="entry name" value="DNA_fragmentation_mid_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02017">
        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09033">
        <property type="entry name" value="DFF-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00266">
        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51135">
        <property type="entry name" value="CIDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CIDE-N" evidence="4">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="227" mass="24730" checksum="419DCA4FA930C6A0" modified="2019-09-18" version="1">MSTCLHAKRENNEISQSSGLEVEDDTPEPGEIWALKQRLLCSNNRREQRGAAASCLEKMRSKSRDVPAIKDGAQADGGDYFLCLPANAKFVTSAGNQWGTHSSDAGTAWLTQESLDRGETDGGAGSEWKNVARLLMEGLYPALPCCRRKSCRRSLMSRVPSWFRTSARAVWLSSSFRTPCSRCLTGERKPSSPGGSRNVPAGLWRRTLASCRSSKSPEPTSGGEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPC7</accession>
    <name>A0A4W2CPC7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Hexosyltransferase</fullName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">B3GALT6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3-O-(beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-galactose = 3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11780"/>
            <dbReference type="Rhea" id="RHEA-COMP:12570"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:132088"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="EC" id="2.4.1.134"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:11781"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; chondroitin sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_027421062.1">
        <property type="nucleotide sequence ID" value="XM_027565261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CPC7"/>
    <dbReference type="SMR" id="A0A4W2CPC7"/>
    <dbReference type="STRING" id="30522.A0A4W2CPC7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046222.1">
        <property type="protein sequence ID" value="ENSBIXP00000013811.1"/>
        <property type="gene ID" value="ENSBIXG00000018676.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001215.1">
        <property type="protein sequence ID" value="ENSBIXP00005032605.1"/>
        <property type="gene ID" value="ENSBIXG00005013144.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906719"/>
    <dbReference type="CTD" id="126792"/>
    <dbReference type="GeneTree" id="ENSGT00940000162229"/>
    <dbReference type="OMA" id="LKPSHGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047220">
        <property type="term" value="F:galactosylxylosylprotein 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050651">
        <property type="term" value="P:dermatan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000018">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF3">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000013811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="329" mass="36950" checksum="2D41E13076E89AE2" modified="2019-09-18" version="1">MKLLRRAWRHRTALGLGGLAMGGVALLYLARCAAPPDSALSGPAAPAPVGPARAAAFLAVLVASAPRAAERRSVVRSTWLAARRGGPGDVWARFAVGTSGLGDEERRALEREQAQHGDLLLLPGLRDAYENLTAKVLAMLAWLDEHVAFEFVLKADDDSFARLDAVLAELRARDPARRRRLYWGFFSGRGRVRPGGRWREAAWQLCDYYLPYALGGGYVLSADLVRYLRLSREYLRAWHSEDVSLGAWLAPVDVQREHDPRFDTEYKSRGCNNQYLVTHKQSLEDMLEKHRTLTHEGRLCKREVQLRLSYVYDWSAPPSQCCQRKEGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ62</accession>
    <name>A0A4W2CQ62_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Adaptor related protein complex 5 subunit zeta 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AP5Z1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQ62"/>
    <dbReference type="STRING" id="30522.A0A4W2CQ62"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016145.1">
        <property type="protein sequence ID" value="ENSBIXP00000008460.1"/>
        <property type="gene ID" value="ENSBIXG00000014299.1"/>
    </dbReference>
    <dbReference type="OMA" id="LMLAYEF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044599">
        <property type="term" value="C:AP-5 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="AP5Z1"/>
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        <property type="entry name" value="ARM-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR46488:SF1">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT ZETA-1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="37"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit zeta-1 ARM repeats" evidence="1">
        <location>
            <begin position="356"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit zeta-1 C-terminal TPR" evidence="3">
        <location>
            <begin position="486"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="849" mass="92241" checksum="08590EE9D3928EAC" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQA1</accession>
    <name>A0A4W2CQA1_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">UBZ2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQA1"/>
    <dbReference type="SMR" id="A0A4W2CQA1"/>
    <dbReference type="STRING" id="30522.A0A4W2CQA1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024877.1">
        <property type="protein sequence ID" value="ENSBIXP00000014135.1"/>
        <property type="gene ID" value="ENSBIXG00000003112.1"/>
    </dbReference>
    <dbReference type="OMA" id="GECARLW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FA_core_complex_assoc"/>
    </dbReference>
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        <property type="entry name" value="FANCA_interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031490">
        <property type="entry name" value="UBZ2_FAAP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37862">
        <property type="entry name" value="FANCONI ANEMIA CORE COMPLEX-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37862:SF1">
        <property type="entry name" value="FANCONI ANEMIA CORE COMPLEX-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15751">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51906">
        <property type="entry name" value="ZF_UBZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBZ2-type" evidence="2">
        <location>
            <begin position="156"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="273" mass="29131" checksum="302FEB41C231C27E" modified="2019-09-18" version="1">MEATRRSRLSLSRRRPPLGVRPRSNTAGSLPDGGECAKLWTELLRTASADLNVDGELPPLPAFPDQEPRRSPERPPPETFTVGTETFFWTPFPAPSPGRGGNSGGSDLVLSAVRGRTGSPQQYAAPELRGTPSAEEQPSEEQPSQRQSSVDGAMTLQSCPMCQVDFAPGLAQLDIDGHLAQCLADSTDDIECDVMTEVDRATQQTDQPQVNLLLQLQGGRAAPGICREGSGPPADVLCELCLGGPVQGVFSPVPSVHRFWKSLIFSSAMAAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQC6</accession>
    <name>A0A4W2CQC6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CTD nuclear envelope phosphatase 1</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serine/threonine-protein phosphatase dullard</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CTDNEP1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 19">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with CNEP1R1; the complex dephosphorylates LPIN1 and LPIN2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Dullard family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027375538.1">
        <property type="nucleotide sequence ID" value="XM_027519737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQC6"/>
    <dbReference type="SMR" id="A0A4W2CQC6"/>
    <dbReference type="STRING" id="30522.A0A4W2CQC6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016227.1">
        <property type="protein sequence ID" value="ENSBIXP00000008521.1"/>
        <property type="gene ID" value="ENSBIXG00000014344.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035466.1">
        <property type="protein sequence ID" value="ENSBIXP00005041468.1"/>
        <property type="gene ID" value="ENSBIXG00005024608.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878542"/>
    <dbReference type="CTD" id="23399"/>
    <dbReference type="GeneTree" id="ENSGT01040000240503"/>
    <dbReference type="OMA" id="RIWGFFM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000044">
        <property type="entry name" value="CTD nuclear envelope phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FCP1 homology" evidence="15">
        <location>
            <begin position="57"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073467"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081325"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="244" mass="28393" checksum="44494AC37D7E2F30" modified="2019-09-18" version="1">MMRTQCLLGLRTFVAFAAKLWSFFIYLLRRQIRTVIQYQTVRYDILPLSPVSRNRLSQVKRKILVLDLDETLIHSHHDGVLRPTVRPGTPPDFILKVVIDKHPVRFFVHKRPHVDFFLEVVSQWYELVVFTASMEIYGSAVADKLDNSRSILKRRYYRQHCTLELGSYIKDLSVVHSDLSSIVILDNSPGAYRSHPDNAIPIKSWFSDPSDTALLNLLPMLDALRFTADVRSVLSRNLHQHRLW</sequence>
</entry>
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    <accession>A0A4W2CQQ7</accession>
    <name>A0A4W2CQQ7_BOBOX</name>
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        <submittedName>
            <fullName evidence="5">WAP four-disulfide core domain 3</fullName>
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        <name evidence="5" type="primary">WFDC3</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elafin-like_sf"/>
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        <property type="entry name" value="WAP_dom"/>
    </dbReference>
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        <property type="entry name" value="WAP_four-disulfide_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19441:SF97">
        <property type="entry name" value="WAP FOUR-DISULFIDE CORE DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19441">
        <property type="entry name" value="WHEY ACDIC PROTEIN WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="96"/>
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    </feature>
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        <location>
            <begin position="192"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="211" mass="22016" checksum="23ED34808D21B629" modified="2019-09-18" version="1">MQGFHSCGVPQTPFCSALEEKTSLGIIAKGKDLGLGLGQSPCLALPSVDLLGKMYMSGRVGVFISALSILVMLSCLFLLKTVVALGSVASRLTVGEREFGGECPADPLPCEELCDGDTSCPQGHKCCSTGCGHACFGDIKGGMSALWGRNPWVLLGSQSWLGREPSVIVFSFCLWEEVKILILPVLIPCPPSSQSNPPPGSRGPCMAGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSW5</accession>
    <name>A0A4W2CSW5_BOBOX</name>
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        <submittedName>
            <fullName evidence="4">RIPOR family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RIPOR3</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RIPOR family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2CSW5"/>
    <dbReference type="STRING" id="30522.A0A4W2CSW5"/>
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        <property type="gene ID" value="ENSBIXG00000003491.1"/>
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    <dbReference type="OMA" id="ELDYLCG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
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        <property type="entry name" value="FAM65_N"/>
    </dbReference>
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        <property type="entry name" value="RIPOR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15829">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PL48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM65 N-terminal" evidence="3">
        <location>
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            <end position="363"/>
        </location>
    </feature>
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        </location>
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        <location>
            <begin position="469"/>
            <end position="514"/>
        </location>
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        <location>
            <begin position="590"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="948" mass="105649" checksum="2B79114D817ED054" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTK0</accession>
    <name>A0A4W2CTK0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="Ensembl" id="ENSBIXT00000046921.1">
        <property type="protein sequence ID" value="ENSBIXP00000015266.1"/>
        <property type="gene ID" value="ENSBIXG00000006642.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:synapse organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="11"/>
    </dbReference>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376329.1">
        <property type="nucleotide sequence ID" value="XM_027520528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CU30"/>
    <dbReference type="SMR" id="A0A4W2CU30"/>
    <dbReference type="STRING" id="30522.A0A4W2CU30"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018269.1">
        <property type="protein sequence ID" value="ENSBIXP00000009792.1"/>
        <property type="gene ID" value="ENSBIXG00000015413.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005005655.1">
        <property type="protein sequence ID" value="ENSBIXP00005005799.1"/>
        <property type="gene ID" value="ENSBIXG00005001098.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879227"/>
    <dbReference type="CTD" id="54622"/>
    <dbReference type="GeneTree" id="ENSGT00940000156244"/>
    <dbReference type="OMA" id="RLCCKGP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04162">
        <property type="entry name" value="Arl9_Arfrp2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000934">
        <property type="entry name" value="ADP-ribosylation factor-like 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042292">
        <property type="entry name" value="ARL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46693">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46693:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="202" mass="22608" checksum="3A07F49E96E2DA33" modified="2019-09-18" version="1">MSDLRISEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDSVVSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLETARNELHSALQHPQLCTLPFLILANHQDKPAARSVQEVKKYFELEPLARGKRWILQPCSLDDMEALKDSFSQLINLLEDHEAVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CW32</accession>
    <name>A0A4W2CW32_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SUMO specific peptidase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SENP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CW32"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030222.1">
        <property type="protein sequence ID" value="ENSBIXP00000017616.1"/>
        <property type="gene ID" value="ENSBIXG00000021618.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000001">
        <property type="entry name" value="Sentrin-specific protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF30">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="451"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="645" mass="73564" checksum="978DC937820EF511" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYG6</accession>
    <name>A0A4W2CYG6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein G</fullName>
            <shortName evidence="9">snRNP-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SNRPG</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. Is also a component of the minor U12 spliceosome. As part of the U7 snRNP it is involved in histone 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027411536.1">
        <property type="nucleotide sequence ID" value="XM_027555735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CYG6"/>
    <dbReference type="SMR" id="A0A4W2CYG6"/>
    <dbReference type="STRING" id="30522.A0A4W2CYG6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031563.1">
        <property type="protein sequence ID" value="ENSBIXP00000018486.1"/>
        <property type="gene ID" value="ENSBIXG00000022329.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014648.1">
        <property type="protein sequence ID" value="ENSBIXP00005028203.1"/>
        <property type="gene ID" value="ENSBIXG00005029371.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901410"/>
    <dbReference type="CTD" id="6637"/>
    <dbReference type="GeneTree" id="ENSGT00510000046985"/>
    <dbReference type="OMA" id="MSKAQPP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034709">
        <property type="term" value="C:methylosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005683">
        <property type="term" value="C:U7 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01719">
        <property type="entry name" value="Sm_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000017">
        <property type="entry name" value="Small nuclear ribonucleoprotein G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034098">
        <property type="entry name" value="Sm_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF26">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="76" mass="8496" checksum="623302BF6BE758FE" modified="2019-09-18" version="1">MSKAHPPELKKFMDKKLSLKLNGGRHVQGILRGFDPFMNLVIDECVEMATSGQQNNIGMVVIRGNSIIMLEALERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZ82</accession>
    <name>A0A4W2CZ82_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SIGLEC family like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZ82"/>
    <dbReference type="STRING" id="30522.A0A4W2CZ82"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021179.1">
        <property type="protein sequence ID" value="ENSBIXP00000011675.1"/>
        <property type="gene ID" value="ENSBIXG00000002735.1"/>
    </dbReference>
    <dbReference type="OMA" id="ACQEPKM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF113">
        <property type="entry name" value="RIKEN CDNA 4931406B18 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="25362" checksum="DCDAC626331C513B" modified="2019-09-18" version="1">MAPPGLRLAPTRLLDSSCSLKKTLQCSCSFRGTPMPSVQWWVGGTPVAMNHTGFRVTSTTLGPWANSTIHLTKLPETGTSLLCEGRNPEGTHALTILLKSGDGGGAPCGWGRGGGPWGLGRQLGGPRGEPLLMSSLPPGRSPLVAQTFMKGLIRGVFYGAIGVTLLFLCLVPLIAKHIRLKQAKKIAAMKAEKSPKRARQGLETSLRLKEPGKSRVTPSPEQQILDPSPQTSKKSRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CZN7</accession>
    <name>A0A4W2CZN7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">X-linked retinitis pigmentosa GTPase regulator-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May function as scaffolding protein. Required for normal location of RPGR at the connecting cilium of photoreceptor cells. Required for normal disk morphogenesis and disk organization in the outer segment of photoreceptor cells and for survival of photoreceptor cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RPGRIP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CZN7"/>
    <dbReference type="STRING" id="30522.A0A4W2CZN7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032361.1">
        <property type="protein sequence ID" value="ENSBIXP00000018980.1"/>
        <property type="gene ID" value="ENSBIXG00000022723.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTLAAGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032391">
        <property type="term" value="C:photoreceptor connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046548">
        <property type="term" value="P:retinal rod cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000073">
        <property type="entry name" value="protein fantom isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000075">
        <property type="entry name" value="protein fantom isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000189">
        <property type="entry name" value="X-linked retinitis pigmentosa GTPase regulator-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021656">
        <property type="entry name" value="C2-C2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041091">
        <property type="entry name" value="RPGRIP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031139">
        <property type="entry name" value="RPGRIP1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14240">
        <property type="entry name" value="RETINITIS PIGMENTOSA GTPASE REGULATOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14240:SF3">
        <property type="entry name" value="X-LINKED RETINITIS PIGMENTOSA GTPASE REGULATOR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11618">
        <property type="entry name" value="C2-C2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18111">
        <property type="entry name" value="RPGR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7" id="KW-0844">Vision</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="746"/>
            <end position="866"/>
        </location>
    </feature>
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            <end position="219"/>
        </location>
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        <location>
            <begin position="890"/>
            <end position="969"/>
        </location>
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        <location>
            <begin position="294"/>
            <end position="429"/>
        </location>
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        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
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        <location>
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            <end position="112"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
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            <end position="151"/>
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        <location>
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            <end position="191"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00055830"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1243" mass="142160" checksum="661DC11BB4F949F5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1W5</accession>
    <name>A0A4W2D1W5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">Pro-MCH</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2D1W5"/>
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        <property type="term" value="F:type 1 melanin-concentrating hormone receptor binding"/>
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        <property type="entry name" value="Prepro-melanin_conc_hormone"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRO-MCH"/>
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    </dbReference>
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        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="124" mass="14326" checksum="D88613D089BF8A66" modified="2019-09-18" version="1">SIRILHNCIIVPFPIEQLKHKSTFSLQNAGSKHNFLNHGLPLNLAIKPYLALKGSVAFPAENEVQNTESTQEKRETWDEENSAKFPIGRRDFDKNYTQYLSGRLQQKYHRCGDLSQFWRLGGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D2P7</accession>
    <name>A0A4W2D2P7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Alpha-beta TR is a heterodimer composed of an alpha and beta chain; disulfide-linked. The alpha-beta TR is associated with the transmembrane signaling CD3 coreceptor proteins to form the TR-CD3 (TcR or TCR). The assembly of alpha-beta TR heterodimers with CD3 occurs in the endoplasmic reticulum where a single alpha-beta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octameric structure. CD3D-CD3E and CD3G-CD3E heterodimers preferentially associate with TR alpha and TR beta chains, respectively. The association of the CD247 homodimer is the last step of TcR assembly in the endoplasmic reticulum and is required for transport to the cell surface.</text>
    </comment>
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    <dbReference type="STRING" id="30522.A0A4W2D2P7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033977.1">
        <property type="protein sequence ID" value="ENSBIXP00000020071.1"/>
        <property type="gene ID" value="ENSBIXG00000002664.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF19">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 6-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021268964" description="Immunoglobulin V-set domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="9">
        <location>
            <begin position="26"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="126" mass="13895" checksum="21942A70ED048C2E" modified="2019-09-18" version="1">VSPCLLGCVVFCLLQAGMVHAGVTQDPRFQVVRRGQSATLKCTQDLGHDCMYWYRQDLGHELRLIHYSAGVHTSEPGDMPDGYSVSRSNTQKFPLTLDSYSTALQGHLLSVEKDKGPTCRILALVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D420</accession>
    <name>A0A4W2D420_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">separase</fullName>
            <ecNumber evidence="2">3.4.22.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ESPL1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="STRING" id="30522.A0A4W2D420"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032350.1">
        <property type="protein sequence ID" value="ENSBIXP00000018969.1"/>
        <property type="gene ID" value="ENSBIXG00000022698.1"/>
    </dbReference>
    <dbReference type="OMA" id="YMGMTAS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
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        <property type="entry name" value="SEPARIN"/>
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        <property type="entry name" value="Separin_C"/>
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        <property type="entry name" value="SEPARIN"/>
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        <location>
            <begin position="1925"/>
            <end position="2020"/>
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            <begin position="1290"/>
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            <begin position="1307"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1431"/>
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            <begin position="1512"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="2100" mass="230143" checksum="CA38B9F31E52046A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4L0</accession>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
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        </location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6J1</accession>
    <name>A0A4W2D6J1_BOBOX</name>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the ANKRD34 family.</text>
    </comment>
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        <property type="gene ID" value="ENSBIXG00000024594.1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24156:SF4">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 34A"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="424"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="494" mass="52282" checksum="38985DB4AD47CAD0" modified="2019-09-18" version="1">MLHTEGHALLRAVGQGKLRLARLLLEGGAYVNEGDAQGETALMAACRARYDDPQNKARMVRYLLEQGADPNIADRLGRTALMHACAGGGGAAVASLLLAHGADPSVRDHAGASALVHALDRGDRETLATLLDACKAKGTEVIIITTDTSPSGTKKTRQYLNSPPSPGVEDPAPAPPSPGVCTSPSEIQLQTAGAGGRGLLSPRAQEEEEKRDVFEFPLPKPPDDPSPSEPLPKPPRHPPKPLKRLNSEPWGLVAPPQPVPPAEGRPGFERLTAEFNGLTLTGRPRLSRRHSTEGPEDPPPWAEKVTGGGPLSRRNTAPEAQESGPSSGLRQKLSRMESVELDTPGNLCPDSPECSRPSLERRRYSASPLTLPLAGSVSSPRQSQESLPGAVSPLSGRRRSPGLLERRGSGTLLLDHIAQTRPGFLPPLNVSPHPPIPDIRPGGRAPSLPAPPQAGAPGSPRTKRKLVRRHSMQTEQIRLLGGFQSLGGPGEPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6V1</accession>
    <name>A0A4W2D6V1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">High affinity immunoglobulin gamma Fc receptor I</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Fc-gamma RI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FCGR1A</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the immunoglobulin superfamily. FCGR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027390787.1">
        <property type="nucleotide sequence ID" value="XM_027534986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D6V1"/>
    <dbReference type="SMR" id="A0A4W2D6V1"/>
    <dbReference type="STRING" id="30522.A0A4W2D6V1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036339.1">
        <property type="protein sequence ID" value="ENSBIXP00000021589.1"/>
        <property type="gene ID" value="ENSBIXG00000024636.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005036852.1">
        <property type="protein sequence ID" value="ENSBIXP00005022483.1"/>
        <property type="gene ID" value="ENSBIXG00005025364.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887765"/>
    <dbReference type="CTD" id="2209"/>
    <dbReference type="GeneTree" id="ENSGT01050000244808"/>
    <dbReference type="OMA" id="VWFHILF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019771">
        <property type="term" value="F:high-affinity IgG receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019864">
        <property type="term" value="F:IgG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001788">
        <property type="term" value="P:antibody-dependent cellular cytotoxicity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042590">
        <property type="term" value="P:antigen processing and presentation of exogenous peptide antigen via MHC class I"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006910">
        <property type="term" value="P:phagocytosis, recognition"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050766">
        <property type="term" value="P:positive regulation of phagocytosis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032760">
        <property type="term" value="P:positive regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001798">
        <property type="term" value="P:positive regulation of type IIa hypersensitivity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001805">
        <property type="term" value="P:positive regulation of type III hypersensitivity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05752">
        <property type="entry name" value="Ig1_FcgammaR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05753">
        <property type="entry name" value="Ig2_FcgammaR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000651">
        <property type="entry name" value="Fc receptor like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000217">
        <property type="entry name" value="High affinity immunoglobulin gamma Fc receptor I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000356">
        <property type="entry name" value="Low affinity immunoglobulin gamma Fc region receptor III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF11">
        <property type="entry name" value="HIGH AFFINITY IMMUNOGLOBULIN GAMMA FC RECEPTOR I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0390">IgG-binding protein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610696" description="High affinity immunoglobulin gamma Fc receptor I" evidence="17">
        <location>
            <begin position="17"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="19"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="95"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="349" mass="39608" checksum="D0B77B2EF9408C02" modified="2019-09-18" version="1">MWLIIALILGAPVAEQVDPTKAVITLKPPWVSVFQEENVTLLCEGPHRPGDTATQWFLNGTAIKTLAPRYSINSATFDDSGEYKCQTGLSMLSDPVQLEIHSDWLLLQVTSRVFTEGDPLALRCHAWKNMPVYKMLFYKDGKPFRFSSQDSEFTILQTNLSHNGIYHCSGERRRRYTSAGVSITIKELFPAPVLRTSFSSPHQEGNLVNLSCETKLPSEKPGQQLYFSFYVGNKTLISRTTSSEYQTFIAKKEDRRLYWCEAATGDGNLIKRSPELELPVLGLQSTTPVWFHFLFYLAVGIMFLVDSVLCIVIHKELQRKKMWNLEIYLDSLDSGHGKEVPPTFKNIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9H4</accession>
    <name>A0A4W2D9H4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/arginine-rich splicing factor 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FUS-interacting serine-arginine-rich protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Splicing factor, arginine/serine-rich 13A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">TLS-associated protein with Ser-Arg repeats</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">TLS-associated serine-arginine protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SRSF10</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376201.1">
        <property type="nucleotide sequence ID" value="XM_027520400.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2D9H4"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="88"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="88"/>
            <end position="102"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="109"/>
            <end position="126"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="134"/>
            <end position="150"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="165"/>
            <end position="186"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="194"/>
            <end position="207"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="209"/>
            <end position="234"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="252"/>
            <end position="262"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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            <dbReference type="ARBA" id="ARBA00067946"/>
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            <dbReference type="ARBA" id="ARBA00084028"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022652.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="262" mass="31287" checksum="4B2F0BA229BBFAB5" modified="2019-09-18" version="1">MSRYLRPPNTSLFVRNVADDTRSEDLRREFGRYGPIVDVYVPLDFYTRRPRGFAYVQFEDVRDAEDALHNLDRKWICGRQIEIQFAQGDRKTPNQMKAKEGRNVYSSSRYDDYDRYRRSRSRSYDRRRSRSRSFDYNYRRSYSPRNSRPTGRPRRSRSHSDNDRFKHRNRSFSRSKSNSRSRSKSQPKKEMKAKSRSRSASHTKTRGTSKTDSKTHYKSGSRYEKESRKKEPPRSKSQSRSQSRSRSKSRSRSWTSPKSSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9Z7</accession>
    <name>A0A4W2D9Z7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclic nucleotide-gated channel alpha-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Cyclic nucleotide-gated channel, photoreceptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Rod photoreceptor cGMP-gated cation channel subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">cGMP-gated cation channel alpha-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="37 38">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="37 38" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="37" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="31">Pore-forming subunit of the rod cyclic nucleotide-gated channel. Mediates rod photoresponses at dim light converting transient changes in intracellular cGMP levels into electrical signals. In the dark, cGMP levels are high and keep the channel open enabling a steady inward current carried by Na(+) and Ca(2+) ions that leads to membrane depolarization and neurotransmitter release from synaptic terminals. Upon photon absorption cGMP levels decline leading to channel closure and membrane hyperpolarization that ultimately slows neurotransmitter release and signals the presence of light, the end point of the phototransduction cascade. Conducts cGMP- and cAMP-gated ion currents, with permeability for monovalent and divalent cations. The selectivity for Ca(2+) over Na(+) increases with cGMP concentrations, whereas the selectivity among monovalent ions is independent of the cGMP levels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>Li(+)(in) = Li(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78551"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NH4(+)(in) = NH4(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28747"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9Z7"/>
    <dbReference type="STRING" id="30522.A0A4W2D9Z7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038097.1">
        <property type="protein sequence ID" value="ENSBIXP00000022801.1"/>
        <property type="gene ID" value="ENSBIXG00000006689.1"/>
    </dbReference>
    <dbReference type="OMA" id="ECEPQTR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="cGMP-gated cation channel alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
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    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
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    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF9">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CHANNEL ROD PHOTORECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="10" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="21" id="KW-0114">cAMP</keyword>
    <keyword evidence="9" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0140">cGMP</keyword>
    <keyword evidence="17" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="22" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="20 35" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="13 35" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 35" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="24" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="36">
        <location>
            <begin position="481"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="37"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="92"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="34">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="115"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00032867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00044635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00044738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00044817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00044828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="694" mass="80058" checksum="FF8029123A345C98" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBB2</accession>
    <name>A0A4W2DBB2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MRTO4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with the pre-60S ribosomal particle. Interacts with MINAS-60 (product of an alternative open reading frame of RBM10).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377869.1">
        <property type="nucleotide sequence ID" value="XM_027522068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBB2"/>
    <dbReference type="SMR" id="A0A4W2DBB2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000023313.1"/>
        <property type="gene ID" value="ENSBIXG00000025810.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052545.1">
        <property type="protein sequence ID" value="ENSBIXP00005043508.1"/>
        <property type="gene ID" value="ENSBIXG00005005607.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880053"/>
    <dbReference type="CTD" id="51154"/>
    <dbReference type="GeneTree" id="ENSGT00390000006238"/>
    <dbReference type="OMA" id="LEWAENY"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000004">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000002">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="10">
        <location>
            <begin position="125"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="239" mass="27526" checksum="C7BEA8C4109FF068" modified="2019-09-18" version="1">MPKSKRDKKVSLTKTAKKGLELKQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKKLRGEVGLLFTNRTKEEVDEWFTKYTEMDYARAGNKATFTVNLDPGPLEQFPHSMEPQLRQLGLPTALKKGVVTLLSDYEVCKEGDVLTPEQARVLKLFGYEMAEFKVSIKYMWDAQSGRFQQMGDDLPESAPESEGESEEEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDA9</accession>
    <name>A0A4W2DDA9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Integrin alpha-6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">CD49 antigen-like family member F</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Integrin alpha-7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">VLA-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">ITGA6</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 (ITGB1) or beta-4 (ITGB4) to form ITGA6:ITGB1 and ITGA6:ITGB4, respectively. ITGA6:ITGB1 is found in a complex with CD9; interaction takes place in oocytes and is involved in sperm-egg fusion. ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3. ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R. ITGA6:ITGB4 interacts with IGF2. Interacts with ADAM9. Interacts with RAB21. Interacts with MDK. ITGA6:ITGB1 interacts with MDK; this interaction mediates MDK-induced neurite outgrowth. Interacts with CD82; this interaction down-regulates ITGA6-mediated cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Membrane</location>
            <topology evidence="28">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 28">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027413365.1">
        <property type="nucleotide sequence ID" value="XM_027557564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DDA9"/>
    <dbReference type="SMR" id="A0A4W2DDA9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050347.1">
        <property type="protein sequence ID" value="ENSBIXP00000022183.1"/>
        <property type="gene ID" value="ENSBIXG00000025114.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902372"/>
    <dbReference type="CTD" id="3655"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000002">
        <property type="entry name" value="integrin alpha-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000002">
        <property type="entry name" value="integrin alpha-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000002">
        <property type="entry name" value="Integrin subunit alpha 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1530:FF:000001">
        <property type="entry name" value="Integrin subunit alpha 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000001">
        <property type="entry name" value="Integrin subunit alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF9">
        <property type="entry name" value="INTEGRIN ALPHA-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 28" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 28" id="KW-0401">Integrin</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 28" id="KW-0732">Signal</keyword>
    <keyword evidence="7 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021452928" description="Integrin alpha-6" evidence="28">
        <location>
            <begin position="24"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="1013"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="27">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="27">
        <location>
            <begin position="101"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="27">
        <location>
            <begin position="301"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="27">
        <location>
            <begin position="364"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="27">
        <location>
            <begin position="420"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="30">
        <location>
            <begin position="464"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="31">
        <location>
            <begin position="626"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="32">
        <location>
            <begin position="797"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00054656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00062424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00068411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00080506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1073" mass="119234" checksum="E1B21477EAF9D733" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DEI6</accession>
    <name>A0A4W2DEI6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP binding cassette subfamily C member 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113902481</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an S-substituted glutathione(in) + ATP + H2O = an S-substituted glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19121"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19122"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Sarcoplasmic reticulum membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DRD8"/>
    <dbReference type="SMR" id="A0A4W2DRD8"/>
    <dbReference type="STRING" id="30522.A0A4W2DRD8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008298.1">
        <property type="protein sequence ID" value="ENSBIXP00000029094.1"/>
        <property type="gene ID" value="ENSBIXG00000010597.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018431.1">
        <property type="protein sequence ID" value="ENSBIXP00005034020.1"/>
        <property type="gene ID" value="ENSBIXG00005014953.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156481"/>
    <dbReference type="OMA" id="QSVQHCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000239">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="350"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="404"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="596" mass="65863" checksum="584038FFB60B3A0A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DSY4</accession>
    <name>A0A4W2DSY4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DSY4"/>
    <dbReference type="STRING" id="30522.A0A4W2DSY4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006331.1">
        <property type="protein sequence ID" value="ENSBIXP00000029688.1"/>
        <property type="gene ID" value="ENSBIXG00000011407.1"/>
    </dbReference>
    <dbReference type="OMA" id="RASICKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000003">
        <property type="entry name" value="Lysine-specific demethylase 4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF21">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="JmjN" evidence="15">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="16">
        <location>
            <begin position="144"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="367"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="500" mass="55865" checksum="3235DAC0C444648C" modified="2019-09-18" version="1">MEAMKPSCAQNPSCSIMIFHPTKEEFTDFDKYIAYIESQGAHRAGLAKIVPPKEWKARQTYDDINDILIAAPLQQVVSGRAGVFTQYHKKKKAMTVAEYRHLANTEKYQTPFYSDFEELERKYWKTRLFESPIYGADISGSLFDENTKQWNLGRLGTIQDLLEQECGVVIEGVNTPYLYFGMWKTAFAWHTEDMDLYSINFLHFGEPKTWYAVPPEHGRRLERLASALFPGSSRGCEAFLRHKAALISPTVLRDNGIPFGRVTQEAGEFMVTFPYGYHSGFNHGFNCAEAINFATPRWIDYGKVASQCSCGEAQVAFSMDAFVRILQPERYELWKRGQDRAVVNHAEPAAPGGQELRTWKEVQTLGPKYFPPRRTARLRQPVSSSEGTDPRAPVRAVSLRPLPARGSCSASQFDAVAGSSSRKPSQTPPLLPGPSVASCLHPVGRCGSRRRPQEKSTQELTAPIGAKRGLALDRKAQDPKAQPLSAEGRMDNPAPTIPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DT73</accession>
    <name>A0A4W2DT73_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ankyrin repeat and SOCS box containing 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ASB18</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DT73"/>
    <dbReference type="STRING" id="30522.A0A4W2DT73"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050392.1">
        <property type="protein sequence ID" value="ENSBIXP00000022260.1"/>
        <property type="gene ID" value="ENSBIXG00000007993.1"/>
    </dbReference>
    <dbReference type="OMA" id="DARNGCG"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000154">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000228">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF18">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="122"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="189"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="8">
        <location>
            <begin position="392"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="437" mass="47613" checksum="07DD0A943AD50FDA" modified="2019-09-18" version="1">MDGQPEPVRGRLQIFQKMEKEPQARDGSPVLEYHAALVTGDLEHLKPLMDKFFQDANVVFEIKKDEMVWQVKSPATFGLSGLWTLEYKRELTTPLCIAAAQGHTACVRHLLGRGADPDASPGGRGALHEACLGSHTACARLLLQHRADPDLLSAEGLAPLHLCRTAASLGCAQALLEHGASVQLEGGTSRDTPLHVAAQRGLDEHARLYLNRGARVDARNGHGETALSAACGAALRPDEHARCLRLCTLLLRHGAAADTRDEDERSPLHKACGHARPGLARLLLLHGADARALDYGGASPLARVLQTAACAPEGEPQLTVQELLNHGSPTVWPDAFPKVLKSCAAVPTVIEVLFNSYSQLRVSESWKDVIPEEVFQMHQAFYRSLFALAHTPRCLQHLCRCAIRKSFGRKCFSLVPLLPLPESLQNYLLLEPEGVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTP4</accession>
    <name>A0A4W2DTP4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycophorin-A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTP4"/>
    <dbReference type="STRING" id="30522.A0A4W2DTP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005481.1">
        <property type="protein sequence ID" value="ENSBIXP00000029965.1"/>
        <property type="gene ID" value="ENSBIXG00000011737.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021451074" description="Glycophorin-A" evidence="2">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9232" checksum="DE35769DA505C9AE" modified="2019-09-18" version="1">MDRKLIFVLLLSGYIFTKPVAAQTPGPPDTPGLPAMVIAILALIAGIIGITIGSVSLFFILQMRNQVSPRVSETPGQTEQKEDLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWB1</accession>
    <name>A0A4W2DWB1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tetratricopeptide repeat domain 36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TTC36</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009215.1">
        <property type="protein sequence ID" value="ENSBIXP00000028793.1"/>
        <property type="gene ID" value="ENSBIXG00000010161.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090394">
        <property type="term" value="P:negative regulation of excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042864">
        <property type="entry name" value="TMEM25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47224">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47224:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="31"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="366" mass="39301" checksum="71A3F209E1072BA7" modified="2019-09-18" version="1">MASYPPAHTSAPAGPSELRLHYVLGWGELAPQIDGQTWAERALRENERHAFTCRVAGGLGTPRLAWYLDGQLQEASTSRLLSVGREAFSGGTSTFTVTAQRAQHELNCSLQDPGSGQSANASVILNVQFKPEIAQVGAKYQEAQGPGLLVILFALVRANPPANVTWIDQDGPVTVNTSDFLVLDAQNYPWLTNHTVQLQLRSLPHNLSVVATNDVGVTSSSLPAPGLLATRVEVPLLGIVVAGGLALGTLVGFSTLVACLVCRKEKKTKGPSRRPSLISSDSNNLKVSNVRLPRENMSLPSNLQLNDLAPGCRGKPADRQMAQDNSRPDLLDPEPGGLLTSRGFIRLPMLGYIYRVSSVSSDEIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0U6</accession>
    <name>A0A4W2E0U6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sialin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">H(+)/nitrate cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">H(+)/sialic acid cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Vesicular excitatory amino acid transporter</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SLC17A5</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for CM101, a polysaccharide produced by group B Streptococcus with antipathoangiogenic properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 nitrate(out) + H(+)(out) = 2 nitrate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71539"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:71540"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>D-glucuronate(out) + H(+)(out) = D-glucuronate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72591"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:72592"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-aspartate(out) = L-aspartate(in)</text>
            <dbReference type="Rhea" id="RHEA:66332"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:66337"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N-acetyl-L-aspartyl-L-glutamate(out) = N-acetyl-L-aspartyl-L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:72599"/>
            <dbReference type="ChEBI" id="CHEBI:76931"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:72600"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-acetylneuraminate(in) + H(+)(in) = N-acetylneuraminate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28987"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35418"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:28989"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027406746.1">
        <property type="nucleotide sequence ID" value="XM_027550945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E0U6"/>
    <dbReference type="STRING" id="30522.A0A4W2E0U6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044063.1">
        <property type="protein sequence ID" value="ENSBIXP00000032442.1"/>
        <property type="gene ID" value="ENSBIXG00000015031.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019666.1">
        <property type="protein sequence ID" value="ENSBIXP00005011011.1"/>
        <property type="gene ID" value="ENSBIXG00005015518.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898086"/>
    <dbReference type="CTD" id="26503"/>
    <dbReference type="GeneTree" id="ENSGT00940000160370"/>
    <dbReference type="OMA" id="RVVTTWF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042880">
        <property type="term" value="F:D-glucuronate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015136">
        <property type="term" value="F:sialic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098700">
        <property type="term" value="P:neurotransmitter loading into synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17381">
        <property type="entry name" value="MFS_SLC17A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000067">
        <property type="entry name" value="sialin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF432">
        <property type="entry name" value="SIALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="27">
        <location>
            <begin position="41"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00056891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00069713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00080244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00081925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="495" mass="54515" checksum="6C8D53C3F26BD462" modified="2019-09-18" version="1">MKSPVSDLAPSDGEEGSDRTPLLQRAPRAETAPVCCSARYNLAFLSFFGFFVLYSLRVNLSVALVDMVDSNTTAKDNRTSYECAEHSAPIKVLRNQTGKKYQWDAETQGWILGSFFYGYIITQIPGGYVASRSGGKLLLGFGVLGTAVFTLFTPLAADFGFGALVALRALEGLGEGVTFPAMHAMWSSWAPPLERSKLLSISYAGAQLGTVVSLPLSGVICYYMNWTYVFYFFGLVGIIWFILWICLVSDTPETHKTITPYEKEYILSSLKNQLSSQKSVPWIPMLKSLPLWAIVVAHFSYNWTFYTLLTLLPTYMKEVLRFNIQENGFLSAVPYLGCWLCMILSGQAADNLRARWNFSTLWVRRVFSLIGMIGPAIFLVAAGFIGCDYSLAVAFLTISTTLGGFCSSGFSINHLDIAPSYAGILLGITNTFATIPGMIGPIIARSLTPENTIGEWQTVFCIAAAINVFGAIFFTLFAKGEVQNWAISDHRGHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E2F8</accession>
    <name>A0A4W2E2F8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 34</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E2F8"/>
    <dbReference type="STRING" id="30522.A0A4W2E2F8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004523.1">
        <property type="protein sequence ID" value="ENSBIXP00000030284.1"/>
        <property type="gene ID" value="ENSBIXG00000012194.1"/>
    </dbReference>
    <dbReference type="OMA" id="NVFQHQR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001025">
        <property type="entry name" value="zinc finger protein 34"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 34 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002214">
        <property type="entry name" value="zinc finger protein 34 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000011">
        <property type="entry name" value="zinc finger protein 615 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000642">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000427">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="16">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
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            <end position="275"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="501" mass="57406" checksum="0548E072B2418E43" modified="2019-09-18" version="1">MLETYRNLVSLGAGLAGPKPEVIAQLEQGDELWVLDMHGAEQPSVDGSAHGTRTENQEVTSGEMLFGRELDPLRGSVLRGPEPGEVHERVREPEGRLDRPGEQRGPRLVTLANEECGLESGGNLRSRSRPVPDQRPHKCDICEQSFEQRSYLNNHKRVHRCKKTNIVHDSGEIFAANLVKEDQKIPVGKRLYYCGCCGKAFRYSANLVKHQRLHSEEKPYKCEECGKAFHQSCELISHRRMHSGEIPYRCDECGKTFNQRPNLMKHQRIHTGEKPYKCSECGKHFSAYSSLIYHQRIHTGEKPYKCSDCGKAFSDGSILIRHRRTHTGEKPYECKECGKGFTQSSNLIQHQRIHTGEKPYKCNECEKAFIQKTKLVEHQRSHTGEKPYECNDCGKVFSQSTHLIQHQRIHTGEKPYKCSECGKAFHNSSRLIHHQRSHHGEKPYKCADCKKAFSQGTYLLQHRRIHTGEKPYTCGECGKAFRHSSNMSQHQRIHLREDFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E443</accession>
    <name>A0A4W2E443_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E443"/>
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    <dbReference type="Ensembl" id="ENSBIXT00000044985.1">
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        <property type="gene ID" value="ENSBIXG00000006347.1"/>
    </dbReference>
    <dbReference type="OMA" id="HNTGARH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF6">
        <property type="entry name" value="ZINC FINGER PROTEIN 385C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
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    </feature>
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            <end position="311"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="516" mass="54787" checksum="03B4E012A97A86DF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4B5</accession>
    <name>A0A4W2E4B5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RNF6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNF12 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4B5"/>
    <dbReference type="SMR" id="A0A4W2E4B5"/>
    <dbReference type="STRING" id="30522.A0A4W2E4B5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053278.1">
        <property type="protein sequence ID" value="ENSBIXP00000026503.1"/>
        <property type="gene ID" value="ENSBIXG00000029905.1"/>
    </dbReference>
    <dbReference type="OMA" id="DISRDHT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16673">
        <property type="entry name" value="RING-H2_RNF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000054">
        <property type="entry name" value="E3 ubiquitin-protein ligase RLIM isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058896">
        <property type="entry name" value="RNF6/12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25914">
        <property type="entry name" value="RNF6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="616"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="177"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="299"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="492"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="669" mass="74011" checksum="82457C4979D98597" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EA85</accession>
    <name>A0A4W2EA85_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EA85"/>
    <dbReference type="STRING" id="30522.A0A4W2EA85"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045048.1">
        <property type="protein sequence ID" value="ENSBIXP00000033778.1"/>
        <property type="gene ID" value="ENSBIXG00000016590.1"/>
    </dbReference>
    <dbReference type="OMA" id="YGPYGWL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153">
        <property type="entry name" value="SELENOPROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153:SF18">
        <property type="entry name" value="SELENOPROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="528" mass="60540" checksum="203DC2D82756F4CE" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EAW9</accession>
    <name>A0A4W2EAW9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ATP4A</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(out) + ATP + H2O + H(+)(in) = K(+)(in) + ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:22044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22045"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">The gastric H(+)/K(+) ATPase pump is composed of the catalytic alpha subunit ATP4A and the regulatory beta subunit ATP4B. Interacts (via the P-domain) with ATP4B (via N-terminus); this interaction stabilizes the lumenal-open E2 conformation state and prevents the reverse reaction of the transport cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
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    <dbReference type="SMR" id="A0A4W2EAW9"/>
    <dbReference type="STRING" id="30522.A0A4W2EAW9"/>
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        <property type="protein sequence ID" value="ENSBIXP00000033117.1"/>
        <property type="gene ID" value="ENSBIXG00000015824.1"/>
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    <dbReference type="OMA" id="PVQKDCD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008900">
        <property type="term" value="F:P-type potassium:proton transporter activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000079">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015127">
        <property type="entry name" value="ATPase_P-typ_H/K-transp_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF10">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE ALPHA CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09040">
        <property type="entry name" value="H-K_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
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    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="16 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 21" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 21" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="965"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="996"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="52"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045621"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1034" mass="114236" checksum="9DEAD2C0637E5D9B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECP6</accession>
    <name>A0A4W2ECP6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECP6"/>
    <dbReference type="STRING" id="30522.A0A4W2ECP6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020493.1">
        <property type="protein sequence ID" value="ENSBIXP00000033706.1"/>
        <property type="gene ID" value="ENSBIXG00000002574.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="141" mass="15817" checksum="AF99405971961400" modified="2019-09-18" version="1">MNQQMLSDFFCSKVLTSFLKMFLDGLRKNMLLLVVLISAKSSEEDSLSRFSNKPGVDSWPTSDDDVLGSETKKKPKPNLAKLMKAFQQSKRNQTEHGIVRRESTNFSENNNSDSEIEDVVENPSQTITWSPGLLSSCLPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDH2</accession>
    <name>A0A4W2EDH2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptic Ras GTPase activating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SYNGAP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2EDH2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005835.1">
        <property type="protein sequence ID" value="ENSBIXP00000029858.1"/>
        <property type="gene ID" value="ENSBIXG00000011602.1"/>
    </dbReference>
    <dbReference type="OMA" id="DPCVNTE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04013">
        <property type="entry name" value="C2_SynGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13375">
        <property type="entry name" value="PH_SynGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05136">
        <property type="entry name" value="RasGAP_DAB2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000001">
        <property type="entry name" value="Ras GTPase-activating protein nGAP isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000010">
        <property type="entry name" value="Ras GTPase-activating protein nGAP isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037779">
        <property type="entry name" value="SynGAP_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF25">
        <property type="entry name" value="RAS_RAP GTPASE-ACTIVATING PROTEIN SYNGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="217"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="242"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="7">
        <location>
            <begin position="444"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="726"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="930"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1032"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1196"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="955"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1114"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1134"/>
            <end position="1149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1300" mass="143724" checksum="750B64AC4FACCCA5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDX2</accession>
    <name>A0A4W2EDX2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA repair protein XRCC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">X-ray repair cross-complementing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">XRCC1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1. Interacts with (poly-ADP-ribosylated) PARP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDX2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021549.1">
        <property type="protein sequence ID" value="ENSBIXP00000034159.1"/>
        <property type="gene ID" value="ENSBIXG00000007290.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17725">
        <property type="entry name" value="BRCT_XRCC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17707">
        <property type="entry name" value="BRCT_XRCC1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000025">
        <property type="entry name" value="DNA repair protein XRCC1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000008">
        <property type="entry name" value="X-ray repair cross complementing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000012">
        <property type="entry name" value="X-ray repair cross complementing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045080">
        <property type="entry name" value="BRCT_XRCC1_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002706">
        <property type="entry name" value="Xrcc1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11370:SF5">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11370">
        <property type="entry name" value="DNA-REPAIR PROTEIN XRCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01834">
        <property type="entry name" value="XRCC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="310"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="534"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="223"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="395"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="629" mass="68503" checksum="B0467C42FF61FEF0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFP1</accession>
    <name>A0A4W2EFP1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFP1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025708.1">
        <property type="protein sequence ID" value="ENSBIXP00000035979.1"/>
        <property type="gene ID" value="ENSBIXG00000019320.1"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="RT_dom"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="544" mass="62828" checksum="E10100F90F91A765" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGF6</accession>
    <name>A0A4W2EGF6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EGF6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026876.1">
        <property type="protein sequence ID" value="ENSBIXP00000036463.1"/>
        <property type="gene ID" value="ENSBIXG00000019947.1"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
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        <property type="entry name" value="RT_dom"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="568" mass="65615" checksum="EAFEE375B390CA60" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJT9</accession>
    <name>A0A4W2EJT9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase greatwall</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Microtubule-associated serine/threonine-protein kinase-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">MASTL</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJT9"/>
    <dbReference type="STRING" id="30522.A0A4W2EJT9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020535.1">
        <property type="protein sequence ID" value="ENSBIXP00000033732.1"/>
        <property type="gene ID" value="ENSBIXG00000016570.1"/>
    </dbReference>
    <dbReference type="OMA" id="VMKKNEM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000278">
        <property type="entry name" value="serine/threonine-protein kinase greatwall isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001219">
        <property type="entry name" value="serine/threonine-protein kinase greatwall isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000277">
        <property type="entry name" value="serine/threonine-protein kinase greatwall isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="35"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="25">
        <location>
            <begin position="838"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="567"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="704"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="708"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00058869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="881" mass="97844" checksum="810121EE57A3AA11" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EKT7</accession>
    <name>A0A4W2EKT7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EKT7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046243.1">
        <property type="protein sequence ID" value="ENSBIXP00000035440.1"/>
        <property type="gene ID" value="ENSBIXG00000018719.1"/>
    </dbReference>
    <dbReference type="OMA" id="XGDEARE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="116"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="374" mass="42845" checksum="CF6C100689A24848" modified="2019-09-18" version="1">MGKTRGLFKKIRDTKGTFHAKMGLIKDRNGMDLTEAEDIKKRWQEYTELYKRDLHDPDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNVKECSNYRTIALISHASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANIHWIMEKAREFQKSIYFCFIDYAKAFDCVDHNKLWKTVKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGGRQGCILSPCLFNLYAEYIMRNTGLEETQAGIKIGGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWETEGQTVETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMB7</accession>
    <name>A0A4W2EMB7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein 641</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZNF641</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional activator. Activates transcriptional activities of SRE and AP-1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMB7"/>
    <dbReference type="SMR" id="A0A4W2EMB7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030391.1">
        <property type="protein sequence ID" value="ENSBIXP00000037970.1"/>
        <property type="gene ID" value="ENSBIXG00000002899.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000770">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000893">
        <property type="entry name" value="Zinc finger protein 641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000911">
        <property type="entry name" value="Zinc finger protein 641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000948">
        <property type="entry name" value="Zinc finger protein 641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001318">
        <property type="entry name" value="zinc finger protein 641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF127">
        <property type="entry name" value="BLOODY FINGERS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="17">
        <location>
            <begin position="95"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="424" mass="47871" checksum="581057DACF3880D8" modified="2019-09-18" version="1">MLSEQTAALGTGWESMNVQVDRAEPQVERGSQEEGPWRTAPGPLEHLCCDLEEEPQSLQEKAQSTPWVPAIPQEGSTGDWEMAAALLAAGSQGLVTIKDVSLCFSQEEWRSLDPSQTDFYGEYVMQENCGIVVSLRFPIPKLDMLSQLEGGEEQWVPDPPDLEERDILKVTYTGDGSEHEGDTPELEAEPPRMLSSVSQDTVLWNPEQDANWDSMPRSSRGMLLGPPFLQEDSFSNLLCSTEMDSLLRPHTCPQCGKQFVWGSHLARHQQTHTGERPYSCLKCEKSFGRRHHLIRHQKTHLHDKPSRCPECGKNFRCNSHLASHQRVHADGKSCKGQEVGESPGARKRQRAPPVPKCHVCTECGKSFGRRHHLVRHWLTHTGEKPFQCPRCEKSFGRKHHLDRHLLTHQGQSPRSSWDRGTSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EMW4</accession>
    <name>A0A4W2EMW4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor MafB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MAFB</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EMW4"/>
    <dbReference type="STRING" id="30522.A0A4W2EMW4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048330.1">
        <property type="protein sequence ID" value="ENSBIXP00000038268.1"/>
        <property type="gene ID" value="ENSBIXG00000022051.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001506.1">
        <property type="protein sequence ID" value="ENSBIXP00005007852.1"/>
        <property type="gene ID" value="ENSBIXG00005001483.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160486"/>
    <dbReference type="OMA" id="PTEQKHH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021599">
        <property type="term" value="P:abducens nerve formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035284">
        <property type="term" value="P:brain segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903575">
        <property type="term" value="P:cornified envelope assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045647">
        <property type="term" value="P:negative regulation of erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007585">
        <property type="term" value="P:respiratory gaseous exchange by respiratory system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021571">
        <property type="term" value="P:rhombomere 5 development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021572">
        <property type="term" value="P:rhombomere 6 development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007379">
        <property type="term" value="P:segment specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033077">
        <property type="term" value="P:T cell differentiation in thymus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048538">
        <property type="term" value="P:thymus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14718">
        <property type="entry name" value="bZIP_Maf_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000016">
        <property type="entry name" value="MAF bZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013592">
        <property type="entry name" value="Maf_TF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08383">
        <property type="entry name" value="Maf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="238"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="348" mass="38631" checksum="4BBC520D099E0C29" modified="2019-09-18" version="1">MAAELSMGPELPTSPLAMEYVNDFDLLKFDVKKEPLGRAERPGRPCTRLQPAGSVSSTPLSTPCSSVPSSPSFSPTEQKTHLEDLYWMASNYQQMNPEALNLTPEDAVEALIGSHPVPQPLQSFDGFRGAHHHHHHHHPHPHHAYPGPGVAHEELGPHAHPHHHHHHQASPPPSSAASPAQQLPTSHPGPGPHAAAAATAAGGSGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFLAQTQTQPSDRCHHSASRTTRWLRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EP16</accession>
    <name>A0A4W2EP16_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-defensin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Has antibacterial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the beta-defensin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EP16"/>
    <dbReference type="STRING" id="30522.A0A4W2EP16"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031817.1">
        <property type="protein sequence ID" value="ENSBIXP00000038594.1"/>
        <property type="gene ID" value="ENSBIXG00000022449.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043505.1">
        <property type="protein sequence ID" value="ENSBIXP00005045329.1"/>
        <property type="gene ID" value="ENSBIXG00005029075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169471"/>
    <dbReference type="OMA" id="NTCWRTK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.360.10">
        <property type="entry name" value="Antimicrobial Peptide, Beta-defensin 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050544">
        <property type="entry name" value="Beta-defensin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025933">
        <property type="entry name" value="Beta_defensin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001">
        <property type="entry name" value="BETA-DEFENSIN 123-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001:SF10">
        <property type="entry name" value="BETA-DEFENSIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13841">
        <property type="entry name" value="Defensin_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4 9" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6 9" id="KW-0211">Defensin</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516469" description="Beta-defensin" evidence="9">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-defensin" evidence="10">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU231113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8076" checksum="3DD4BBD104B55A95" modified="2019-09-18" version="1">GASVLLTVCLILFLLAVKSGANAFIRRFFNTCWRTKGICRKSCHKSEIFHVFCDVSILCCIDKKHLPSMVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERV5</accession>
    <name>A0A4W2ERV5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ERV5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034301.1">
        <property type="protein sequence ID" value="ENSBIXP00000039694.1"/>
        <property type="gene ID" value="ENSBIXG00000023631.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020081.1">
        <property type="protein sequence ID" value="ENSBIXP00005034724.1"/>
        <property type="gene ID" value="ENSBIXG00005015738.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="99" mass="9970" checksum="14FB82B662D6D5D6" modified="2019-09-18" version="1">MAPRVPGARSQVRGGPAPQRATSAGATLSLSRGGGGRGNPPGAGRRTFRTPAQPPAHLGGTALPWAPGGAACGDRGYPTRVPEGRHHLAPTLRAAPRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETI4</accession>
    <name>A0A4W2ETI4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytosolic carboxypeptidase 1</fullName>
            <ecNumber evidence="16">3.4.17.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">ATP/GTP-binding protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Protein deglutamylase CCP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">AGTPBP1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Metallocarboxypeptidase that mediates protein deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of alpha-tubulin as well as non-tubulin proteins such as MYLK. Involved in KLF4 deglutamylation which promotes KLF4 proteasome-mediated degradation, thereby negatively regulating cell pluripotency maintenance and embryogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60004"/>
            <dbReference type="Rhea" id="RHEA-COMP:15519"/>
            <dbReference type="Rhea" id="RHEA-COMP:15675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:60005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63792"/>
            <dbReference type="Rhea" id="RHEA-COMP:16435"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:149555"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:63793"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with MYLK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 23">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.24" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ETI4"/>
    <dbReference type="STRING" id="30522.A0A4W2ETI4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035625.1">
        <property type="protein sequence ID" value="ENSBIXP00000040247.1"/>
        <property type="gene ID" value="ENSBIXG00000024307.1"/>
    </dbReference>
    <dbReference type="OMA" id="LEYNMPS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06906">
        <property type="entry name" value="M14_Nna1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000024">
        <property type="entry name" value="ATP/GTP binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000125">
        <property type="entry name" value="cytosolic carboxypeptidase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3120:FF:000001">
        <property type="entry name" value="cytosolic carboxypeptidase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033852">
        <property type="entry name" value="CBPC1/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF24">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25571">
        <property type="entry name" value="TPR_CCP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
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        <property type="term" value="F:PDZ domain binding"/>
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        <property type="term" value="F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential"/>
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        <property type="term" value="P:cerebellar granule cell differentiation"/>
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        <property type="term" value="P:excitatory postsynaptic potential"/>
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        <property type="term" value="P:excitatory synapse assembly"/>
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        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
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    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
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    <dbReference type="GO" id="GO:1900454">
        <property type="term" value="P:positive regulation of long-term synaptic depression"/>
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        <property type="term" value="P:prepulse inhibition"/>
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        <property type="term" value="P:regulation of neuron apoptotic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of neuron projection development"/>
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        <property type="term" value="P:regulation of postsynaptic density assembly"/>
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        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
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    <dbReference type="GO" id="GO:0099538">
        <property type="term" value="P:synaptic signaling via neuropeptide"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06391">
        <property type="entry name" value="PBP1_iGluR_delta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13731">
        <property type="entry name" value="PBP2_iGluR_delta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000024">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.2300:FF:000068">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1b"/>
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        <property type="entry name" value="Glutamate receptor, ionotropic, delta 2"/>
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    <dbReference type="FunFam" id="3.40.190.10:FF:000040">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
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    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
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        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
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        <property type="entry name" value="Iontro_rcpt_C"/>
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    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NMDARECEPTOR"/>
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    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
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    </dbReference>
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    <keyword evidence="2 22" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 22" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6 22" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 22" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516488" description="Glutamate receptor" evidence="22">
        <location>
            <begin position="24"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="567"/>
            <end position="587"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="636"/>
            <end position="658"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="831"/>
            <end position="855"/>
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    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="23">
        <location>
            <begin position="442"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="24">
        <location>
            <begin position="452"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="525"/>
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        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="742"/>
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        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="20">
        <location>
            <position position="665"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="20">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="83"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="756"/>
            <end position="811"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="15">
        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056512"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061411"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042666.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1007" mass="113273" checksum="CB36BFE96EB475F8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F216</accession>
    <name>A0A4W2F216_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PPP3CA</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027399773.1">
        <property type="nucleotide sequence ID" value="XM_027543972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F216"/>
    <dbReference type="SMR" id="A0A4W2F216"/>
    <dbReference type="STRING" id="30522.A0A4W2F216"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051938.1">
        <property type="protein sequence ID" value="ENSBIXP00000043089.1"/>
        <property type="gene ID" value="ENSBIXG00000027134.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030578.1">
        <property type="protein sequence ID" value="ENSBIXP00005039316.1"/>
        <property type="gene ID" value="ENSBIXG00005021728.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894084"/>
    <dbReference type="CTD" id="5530"/>
    <dbReference type="GeneTree" id="ENSGT00940000156306"/>
    <dbReference type="OMA" id="YPAACNF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005955">
        <property type="term" value="C:calcineurin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Oxidoreductase_NAD-binding"/>
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        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
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    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46505">
        <property type="entry name" value="OXIDOREDUCTASE NAD-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46505:SF1">
        <property type="entry name" value="OXIDOREDUCTASE NAD-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHEHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="4">
        <location>
            <begin position="47"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="309" mass="34597" checksum="C7AA013E4C725AEA" modified="2019-09-18" version="1">MVVVIPRLLRGSLGAICTQAALLRSTPSAFRYLTRSSVMKSKRRPDHLERTADVVRQEVVSAAKVCGVASESPSVKRLCLLVADKDFSFKAGQWVDFFIPGVSVVGGFSICSSPRLLEQERMIELAVKHANHPPALWIHNQCTLDSEVAVRVGGEFFFDPKPTDASRNLVLIAGGVGINPLLSILRHAADLLRERASKGQGYEMGTVRLLYSAKDTSELLFKKNILDLVNEFPEQIACSLHVTKQTTQITADLRPYITEGRITQKEIRDHISKETLFYICGPPPMTDFFSKELESSRVPREHICFEKWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F4B8</accession>
    <name>A0A4W2F4B8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F4B8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052876.1">
        <property type="protein sequence ID" value="ENSBIXP00000044296.1"/>
        <property type="gene ID" value="ENSBIXG00000028464.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="331" mass="37765" checksum="446BFEB3E6E1CE7F" modified="2019-09-18" version="1">MGIPDHLTCLLRNLYAAQEATVRTGHGTTDWFQIRKGVHQGCVLSPCLFNFYAEYIMRNAGLEEAQAGIKIAGRNLNNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKTKIMASSPITSWETDGETVETVTDYILGFKITADGNCSHEIKRRLLLGRKVMTNLDSVLKSRDTTLPRKVCLVKAMVFPVVMYGCESWTGKKAKCQRIDAFELWCWRRLLRVPWTARRSNLSVLREISPEYSLEGLMLKLKLQYFGHLMQRTDSLDKTLLLGKIEGGRRWGRQKMKWLDGITDSMDMSLSKLRELVMDREAWRAAACGVAKSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F5F6</accession>
    <name>A0A4W2F5F6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Heterogeneous nuclear ribonucleoproteins A2/B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F5F6"/>
    <dbReference type="SMR" id="A0A4W2F5F6"/>
    <dbReference type="STRING" id="30522.A0A4W2F5F6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052840.1">
        <property type="protein sequence ID" value="ENSBIXP00000044235.1"/>
        <property type="gene ID" value="ENSBIXG00000028413.1"/>
    </dbReference>
    <dbReference type="OMA" id="NRYSENN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000040">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein A2/B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000108">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoproteins A2/B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026:SF13">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS A2_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026">
        <property type="entry name" value="HOMOLOGOUS TO DROSOPHILA SQD (SQUID) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="29"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="120"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="230"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000044235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="372" mass="40418" checksum="5271B0E742639645" modified="2019-09-18" version="1">MAQATKRAEKETSTSDPSERNKRDNEKFRKLFVGGLSSETTEETLWNYYRQWGYLTDCVVIRDPASQTSRRFAFVTFSSMAEVDAAMAARPHSIDGKRVAPKRAVPREDYGIPGALVTVKKLFVCKIGEDTEKHHLRDYFGKYGKINAIEIISDRESGRKRGFGFVTFDDHDPVDKLVLQKYHTINGHHAEVRKALSRPEMQELQHSRIGRGGRKADFLYLHGGGGNFGAGRGRDFREASDGSGSSGESGDGDHGYGGGPGGGSLRVSPGYGEGRGGCDGEGPGHGYQDRGCRAGYINYGGRNDGSQNCNNLGNYKQKPSKYELLKSGKLSGRRNMGRSYGGANYCPGGHGGSGGYGETAMLSLLFGRGLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F6M5</accession>
    <name>A0A4W2F6M5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Surfeit locus protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED22</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027412601.1">
        <property type="nucleotide sequence ID" value="XM_027556800.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027412602.1">
        <property type="nucleotide sequence ID" value="XM_027556801.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027412603.1">
        <property type="nucleotide sequence ID" value="XM_027556802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F6M5"/>
    <dbReference type="SMR" id="A0A4W2F6M5"/>
    <dbReference type="STRING" id="30522.A0A4W2F6M5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009607.1">
        <property type="protein sequence ID" value="ENSBIXP00000003556.1"/>
        <property type="gene ID" value="ENSBIXG00000009980.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015229.1">
        <property type="protein sequence ID" value="ENSBIXP00005000930.1"/>
        <property type="gene ID" value="ENSBIXG00005029700.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901834"/>
    <dbReference type="CTD" id="6837"/>
    <dbReference type="GeneTree" id="ENSGT00390000004339"/>
    <dbReference type="OMA" id="KQAECDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="166"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="201" mass="22381" checksum="477D4242E3A84684" modified="2019-09-18" version="1">MAQQRALPQSKETLLQSYNKRLKDDVKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLIALRDEVSIDLYELEEEYYSSSSSLCEANDLPLCEAYWRLDPDTDSTDCLSAPLLASPEPSAGGPLQAAAPTHSHAGGPGPTEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FAP4</accession>
    <name>A0A4W2FAP4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="16">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:25426"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:17975"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2FAP4"/>
    <dbReference type="SMR" id="A0A4W2FAP4"/>
    <dbReference type="STRING" id="30522.A0A4W2FAP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038423.1">
        <property type="protein sequence ID" value="ENSBIXP00000041428.1"/>
        <property type="gene ID" value="ENSBIXG00000002092.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013292.1">
        <property type="protein sequence ID" value="ENSBIXP00005001911.1"/>
        <property type="gene ID" value="ENSBIXG00005008348.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155028"/>
    <dbReference type="OMA" id="MQWRVAP"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000019">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF6">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 16" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="17">
        <location>
            <begin position="42"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="218" mass="24575" checksum="DB86124815B41583" modified="2019-09-18" version="1">MATCSSSIVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMHVMGGHHIVALCVLKGGYKFFADLLDYIKVLNRNSDRSIPMTVDFIRLKSYYNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQALLSLVKRHNPKMVKVASLLVKRTPRSVSYRPDFVGFEIPDKFVVGYALDYNEHFRDLNHVCVISETGKAKYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FJ57</accession>
    <name>A0A4W2FJ57_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acetyl-coenzyme A transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Solute carrier family 33 member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SLC33A1</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>acetyl-CoA(in) = acetyl-CoA(out)</text>
            <dbReference type="Rhea" id="RHEA:75039"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:75040"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SLC33A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027398758.1">
        <property type="nucleotide sequence ID" value="XM_027542957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FJ57"/>
    <dbReference type="STRING" id="30522.A0A4W2FJ57"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050690.1">
        <property type="protein sequence ID" value="ENSBIXP00000022865.1"/>
        <property type="gene ID" value="ENSBIXG00000002146.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006377.1">
        <property type="protein sequence ID" value="ENSBIXP00005005433.1"/>
        <property type="gene ID" value="ENSBIXG00005011151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893177"/>
    <dbReference type="CTD" id="9197"/>
    <dbReference type="GeneTree" id="ENSGT00940000154019"/>
    <dbReference type="OMA" id="RRKSWIM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000287">
        <property type="entry name" value="acetyl-coenzyme A transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="550" mass="61131" checksum="BC2E349BC66A0AF3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FS55</accession>
    <name>A0A4W2FS55_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 24 25">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="14 24 25">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CDS2</name>
    </gene>
    <organism evidence="27 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77126"/>
            <dbReference type="ChEBI" id="CHEBI:85351"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45657"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45672"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77128"/>
            <dbReference type="ChEBI" id="CHEBI:85355"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:85356"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:45677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:72864"/>
            <dbReference type="ChEBI" id="CHEBI:85350"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:45653"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45668"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77130"/>
            <dbReference type="ChEBI" id="CHEBI:85354"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:45669"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:85349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45649"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45660"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77098"/>
            <dbReference type="ChEBI" id="CHEBI:85352"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:45661"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45664"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74560"/>
            <dbReference type="ChEBI" id="CHEBI:85353"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16230"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 24 25">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 24 25">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="14 24 25"/>
    <dbReference type="RefSeq" id="XP_027415225.1">
        <property type="nucleotide sequence ID" value="XM_027559424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FS55"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026082.1">
        <property type="protein sequence ID" value="ENSBIXP00000014910.1"/>
        <property type="gene ID" value="ENSBIXG00000019524.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016129.1">
        <property type="protein sequence ID" value="ENSBIXP00005008589.1"/>
        <property type="gene ID" value="ENSBIXG00005013645.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903370"/>
    <dbReference type="CTD" id="8760"/>
    <dbReference type="GeneTree" id="ENSGT00940000158877"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF4">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="20 24" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="18 24 25" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22 24" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="23 24" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 24 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="17 24 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00000859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00001056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00001617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00001729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00001902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005008589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="51357" checksum="788FC1B80D41902E" modified="2019-09-18" version="1">MTELRQRVAREPEAPPEDKESESEAKADGETASDSESRVEAVTQPPSADDTPEVLNRALSNLSSRWKNWWVRGILTLAMIAFFFIIIYLGPMVLMMIVMCVQIKCFHEIITIGYNVYHSYDLPWFRTLSWYFLLCVNYFFYGETVTDYFFTLVQREEPLRILSKYHRFISFTLYLTGFCMFVLSLVKKHYRLQFYMFGWTHVTLLIVVTQSHLVIHNLFEGMIWFIVPISCVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFATVVFGLLLSYVMSGYRCFVCPVEYNNDTNSFTVDCEPSGLFRLQEYNIPGVIQSIIGWKTVRMYPFQIHSIALSTFASLIGPFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVNVYIASFIRGPNPSKLVQQFLTLRPDQQLHIFNTLKSHLTDKGMLTAATEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GBL3</accession>
    <name>A0A4W2GBL3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bile acid receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Farnesoid X-activated receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Farnesol receptor HRR-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Nuclear receptor subfamily 1 group H member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">NR1H4</name>
    </gene>
    <organism evidence="28 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 25">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397839.1">
        <property type="nucleotide sequence ID" value="XM_027542038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GBL3"/>
    <dbReference type="SMR" id="A0A4W2GBL3"/>
    <dbReference type="STRING" id="30522.A0A4W2GBL3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042420.1">
        <property type="protein sequence ID" value="ENSBIXP00000043585.1"/>
        <property type="gene ID" value="ENSBIXG00000027734.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005026210.1">
        <property type="protein sequence ID" value="ENSBIXP00005015378.1"/>
        <property type="gene ID" value="ENSBIXG00005019293.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892755"/>
    <dbReference type="CTD" id="9971"/>
    <dbReference type="GeneTree" id="ENSGT00940000158037"/>
    <dbReference type="OMA" id="XISDEYI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032052">
        <property type="term" value="F:bile acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038186">
        <property type="term" value="F:bile acid nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903413">
        <property type="term" value="P:cellular response to bile acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038185">
        <property type="term" value="P:nuclear receptor-mediated bile acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06962">
        <property type="entry name" value="NR_DBD_FXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06936">
        <property type="entry name" value="NR_LBD_Fxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000018">
        <property type="entry name" value="Bile acid receptor isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000021">
        <property type="entry name" value="bile acid receptor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044114">
        <property type="entry name" value="NR_LBD_NR1H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF155">
        <property type="entry name" value="BILE ACID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="15 25" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="19" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="20 25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="17 25" id="KW-0804">Transcription</keyword>
    <keyword evidence="14 25" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="26">
        <location>
            <begin position="134"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="27">
        <location>
            <begin position="262"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00071503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005015378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="486" mass="55868" checksum="FD7B5D8E974C9F2E" modified="2019-09-18" version="1">MVMQFQELENPVQISPCHSHTSSGFAMEVMSMKPAKGVLTEQVAGPLGQNLEVEPYSQYNNVQFPQVQPQISSSSYYSNVGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVVEIPITKKARLGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQAIHEDSEGRDLRQVTSTTKSCREKTELTPDQQNLLHYIMDSYSKQRMPQEITNKFLKEEFSAEENFIILTEMATSHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFSKKLPAGHTDLLEERIRKSGISDEYITPMFSFYKSVAELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHADMLMSWRVNDHKFTPLLCEIWDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIN4</accession>
    <name>A0A4W2GIN4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trefoil factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TFF2</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402657.1">
        <property type="nucleotide sequence ID" value="XM_027546856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GIN4"/>
    <dbReference type="SMR" id="A0A4W2GIN4"/>
    <dbReference type="STRING" id="30522.A0A4W2GIN4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052046.1">
        <property type="protein sequence ID" value="ENSBIXP00000043235.1"/>
        <property type="gene ID" value="ENSBIXG00000005728.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030610.1">
        <property type="protein sequence ID" value="ENSBIXP00005018299.1"/>
        <property type="gene ID" value="ENSBIXG00005003655.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895535"/>
    <dbReference type="CTD" id="7032"/>
    <dbReference type="GeneTree" id="ENSGT00940000161334"/>
    <dbReference type="OMA" id="ITSEQCF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031723">
        <property type="term" value="F:CXCR4 chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070098">
        <property type="term" value="P:chemokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030277">
        <property type="term" value="P:maintenance of gastrointestinal epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060455">
        <property type="term" value="P:negative regulation of gastric acid secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.110.10:FF:000005">
        <property type="entry name" value="Trefoil factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.110.10:FF:000001">
        <property type="entry name" value="Trefoil factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017994">
        <property type="entry name" value="P_trefoil_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017957">
        <property type="entry name" value="P_trefoil_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13826">
        <property type="entry name" value="INTESTINAL TREFOIL FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13826:SF17">
        <property type="entry name" value="TREFOIL FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00680">
        <property type="entry name" value="PTREFOIL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00025">
        <property type="entry name" value="P_TREFOIL_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611655" description="Trefoil factor 2" evidence="9">
        <location>
            <begin position="28"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="10">
        <location>
            <begin position="33"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="10">
        <location>
            <begin position="83"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="133" mass="14706" checksum="5DB0B3156654724F" modified="2019-09-18" version="1">MNLDMGPRGTWLLAALLLLGLCTLAGAQKPDACQCSRLNPHNRVNCGFPGISSDDCFSRGCCFDSSVARVPWCFNPLPKQESEECVMEVSARKDCGYPGISPEECESRKCCFSNTIPKVPWCFFPISVEDCHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GKB8</accession>
    <name>A0A4W2GKB8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HAO2</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027391413.1">
        <property type="nucleotide sequence ID" value="XM_027535612.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027391417.1">
        <property type="nucleotide sequence ID" value="XM_027535616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GKB8"/>
    <dbReference type="SMR" id="A0A4W2GKB8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035567.1">
        <property type="protein sequence ID" value="ENSBIXP00000040227.1"/>
        <property type="gene ID" value="ENSBIXG00000024267.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005030971.1">
        <property type="protein sequence ID" value="ENSBIXP00005018546.1"/>
        <property type="gene ID" value="ENSBIXG00005021914.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113888538"/>
    <dbReference type="CTD" id="51179"/>
    <dbReference type="GeneTree" id="ENSGT00390000018717"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 9" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="248"/>
        </location>
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            <begin position="77"/>
            <end position="79"/>
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        <ligand>
            <name>FMN</name>
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            <name>FMN</name>
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            <name>FMN</name>
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        <location>
            <position position="165"/>
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        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
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        <location>
            <position position="224"/>
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            <name>FMN</name>
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            <name>FMN</name>
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            <name>glyoxylate</name>
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            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
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        <location>
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            <name>FMN</name>
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            <name>FMN</name>
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            <dbReference type="ARBA" id="ARBA00001917"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    </evidence>
    <sequence length="353" mass="39253" checksum="E20D09034252F692" modified="2019-09-18" version="1">MPLVCLTDFREHAREHLSKSTWDFIEGGADDCCTRDENMAAFKKIRLRPRYLKDVSKVDMRTTIQGAEISAPICIAPTGFHRLAWPDGEMSTARAAQAASICYITSTYASCSLEDIVAAAPRGLRWFQLYVHPNRQINKQMIQKVESLGFKALVITVDVPKVGNRRNDITNQVDLMKKLLLKDLGSPEMGNVMPYFQMSPIDPSICWEDLSWFQSMTRLPIILKGILTKEDAELAVKHNVHGIIVSNHGGRQLDEVPASIDALTEVVAAVKGKVEVYLDGGIRTGNDVLKALALGAKCVFVGRPILWGLAYKGEHGVKEVLDILKNEFHTSMTLTGCRSVAEINQDLIQFSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GND5</accession>
    <name>A0A4W2GND5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neuropilin and tolloid-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NETO1</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. Interacts with GRIN2A and GRIN2B via its CUB domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic density membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381644.1">
        <property type="nucleotide sequence ID" value="XM_027525843.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027381645.1">
        <property type="nucleotide sequence ID" value="XM_027525844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GND5"/>
    <dbReference type="SMR" id="A0A4W2GND5"/>
    <dbReference type="STRING" id="30522.A0A4W2GND5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013928.1">
        <property type="protein sequence ID" value="ENSBIXP00000001486.1"/>
        <property type="gene ID" value="ENSBIXG00000028505.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031019.1">
        <property type="protein sequence ID" value="ENSBIXP00005018582.1"/>
        <property type="gene ID" value="ENSBIXG00005021949.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882748"/>
    <dbReference type="CTD" id="81832"/>
    <dbReference type="GeneTree" id="ENSGT00940000156700"/>
    <dbReference type="OMA" id="KPLVQPM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060076">
        <property type="term" value="C:excitatory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140231">
        <property type="term" value="P:anterograde axonal transport of neurotransmitter receptor complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:neurotransmitter receptor localization to postsynaptic specialization membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000463">
        <property type="term" value="P:positive regulation of excitatory postsynaptic potential"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of long-term neuronal synaptic plasticity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:visual learning"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuropilin and tolloid-like protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF27">
        <property type="entry name" value="NEUROPILIN AND TOLLOID-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611707" description="Neuropilin and tolloid-like protein 1" evidence="22">
        <location>
            <begin position="23"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="41"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="172"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="533" mass="60291" checksum="A771CCA157BB7793" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GSW4</accession>
    <name>A0A4W2GSW4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GSW4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033206.1">
        <property type="protein sequence ID" value="ENSBIXP00000039189.1"/>
        <property type="gene ID" value="ENSBIXG00000023135.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034409.1">
        <property type="protein sequence ID" value="ENSBIXP00005020872.1"/>
        <property type="gene ID" value="ENSBIXG00005023877.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271425"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="23" mass="2449" checksum="0F6AA5AF937BBB1B" modified="2019-09-18" version="1">GFSSGQKLVFGSGTMLKVNLSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H1Z5</accession>
    <name>A0A4W2H1Z5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit theta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">CCT-theta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CCT8</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with DNAAF4. Interacts with synaptic plasticity regulator PANTS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027397620.1">
        <property type="nucleotide sequence ID" value="XM_027541819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H1Z5"/>
    <dbReference type="STRING" id="30522.A0A4W2H1Z5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040607.1">
        <property type="protein sequence ID" value="ENSBIXP00000024444.1"/>
        <property type="gene ID" value="ENSBIXG00000026605.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038844.1">
        <property type="protein sequence ID" value="ENSBIXP00005023827.1"/>
        <property type="gene ID" value="ENSBIXG00005005570.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892653"/>
    <dbReference type="CTD" id="10694"/>
    <dbReference type="GeneTree" id="ENSGT00550000074783"/>
    <dbReference type="OMA" id="WGLKYAV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000083">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14 21" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00093360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00093509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="553" mass="60203" checksum="C3692D88376DE2F7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HCC9</accession>
    <name>A0A4W2HCC9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulating synaptic membrane exocytosis 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RIMS3</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2HCC9"/>
    <dbReference type="STRING" id="30522.A0A4W2HCC9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050548.1">
        <property type="protein sequence ID" value="ENSBIXP00000022577.1"/>
        <property type="gene ID" value="ENSBIXG00000005193.1"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50004"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027844.1"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="302" mass="32149" checksum="6259573DEF2862E2" modified="2019-09-18" version="1">MFNGESGPASAAASRNVVRSSSISGEICGSQQAGDGTGTSTAKKRRSSLGAKMVAIVGLTQWSKGTLQLPQAEGATKKLRSNIRRSTETGVAVEMRSRVPRQGSRESTDGSTNSNSSDGTFIFPTTRLGAESQFSDFLDGLGPAQIVGRQTLATPPMGDVHIAIMDRSGQLEVEVIEARGLTPKPGSKSLPATYIKVYLLENGACLAKKKTKVAKKTCDPLYQQALLFDEGPQGKVLQVIVWGDYGRMDHKCFMGMAQIMLDELDLSAAVTGWIRMGLKPGPDHLPPKKTAHGQNVGEGNKN</sequence>
</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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        <text evidence="1">Belongs to the PRKRIP1 family.</text>
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        <property type="component" value="Chromosome 25"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein kinase binding"/>
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        <property type="project" value="TreeGrafter"/>
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            <begin position="170"/>
            <end position="179"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010717"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005030715.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="186" mass="21248" checksum="3C8237753153C7C7" modified="2019-09-18" version="1">MASSAASSVRPPRPKKEPQALIIPKNAAEEQKLKLERLMKNPDKAVPIPEKMSEWAPRPPPEFVRDVMGSSAGAGSGEFHVYRHLRRREYQRQDYMDAMAEKQKLDAEFQKRLERNKIAAEEQTAKRRKKRQKLKEKKLLAKKMKLEQKKQSEASSETQEQPSSGSEEASGTEDEEEDAPSFITGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HNF3</accession>
    <name>A0A4W2HNF3_BOBOX</name>
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        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 10-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">LOC113886372</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027532536.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000028065.1"/>
        <property type="gene ID" value="ENSBIXG00000009125.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005031627.1"/>
        <property type="gene ID" value="ENSBIXG00005006329.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000163667"/>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS_OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000023">
        <property type="entry name" value="Solute carrier family 22 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="492"/>
            <end position="508"/>
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    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="92"/>
            <end position="514"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005031627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="546" mass="61401" checksum="41B71B40EC6978F9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HWT6</accession>
    <name>A0A4W2HWT6_BOBOX</name>
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        <submittedName>
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        <name evidence="4" type="primary">MYOZ1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the myozenin family.</text>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005034338.1"/>
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00950000183027"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:actinin binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:molecular condensate scaffold activity"/>
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        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:telethonin binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:sarcomere organization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:skeletal muscle fiber adaptation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:skeletal muscle tissue development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:wound healing"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008438">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941:SF11">
        <property type="entry name" value="MYOZENIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="105"/>
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        </location>
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        <location>
            <begin position="118"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="136"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005034338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="306" mass="32391" checksum="245CED8C2EC2B6A5" modified="2019-09-18" version="1">MPLSGTPAPNKKRKSSKLIMELTGGGQESSGLNLGKKISVPRDVMLEELSLLTNRGSKMFKLRQLRVEKFIYENHPDVFSDSSMDRFQKFIPTVGGQLGTAGQGFSYSKSSGGGQAGRSGSAGQYGSDQQHHHQGSGSGSGSGSGPGSGGAGGPGGHSGRGGAAGTPGVGETGTDNQAGGEGKHITVFKTYISPWEKAMGIDPQQKVELGIDLLAYGAKAELPKYKSFNRTAMPYGGYEKASKRMTFQMPKFDLGPLLSEPLVLYNQNLSNRPSFNRTPIPWLSSGEPVDYNVDISIPLDGETEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HYA5</accession>
    <name>A0A4W2HYA5_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">CKLF like MARVEL transmembrane domain containing 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CMTM6</name>
    </gene>
    <organism evidence="8 10">
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        <lineage>
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            <taxon>Pecora</taxon>
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    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027522271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HYA5"/>
    <dbReference type="STRING" id="30522.A0A4W2HYA5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010818.1">
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        <property type="gene ID" value="ENSBIXG00000009401.1"/>
    </dbReference>
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        <property type="gene ID" value="ENSBIXG00005018160.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000157911"/>
    <dbReference type="OMA" id="ASMVWEK"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:early endosome membrane"/>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of protein stability"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="32"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20220" checksum="FB8CCFE403FE0AD3" modified="2019-09-18" version="1">MENGQVYRATTEAHPGPGIGPRSGLAAYFTLSRLTGFRRLKLLELVLSLLAFICEEVVSQCTLCGGLYFFEFVSCSAFLLSLLILVVYCTPVYDRVDPAKVKSSDFFITLGTGLVFFVASLTFTLTHDNTIAEIAAIVFGMLASLTYLAEFSFMLREKLKEPRPRKGEATMRREHLTEPLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2I3U0</accession>
    <name>A0A4W2I3U0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CREB3 regulatory factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CREBRF</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027376380.1">
        <property type="nucleotide sequence ID" value="XM_027520579.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027520580.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_027520581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I3U0"/>
    <dbReference type="STRING" id="30522.A0A4W2I3U0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018038.1">
        <property type="protein sequence ID" value="ENSBIXP00000032659.1"/>
        <property type="gene ID" value="ENSBIXG00000015289.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047552.1">
        <property type="protein sequence ID" value="ENSBIXP00005037250.1"/>
        <property type="gene ID" value="ENSBIXG00005002650.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879259"/>
    <dbReference type="CTD" id="153222"/>
    <dbReference type="GeneTree" id="ENSGT00390000007125"/>
    <dbReference type="OMA" id="MYQKNGP"/>
    <dbReference type="Proteomes" id="UP000314981">
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        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Acts as a positive regulator of the TORC1 signaling pathway by mediating 'Lys-63'-linked deubiquitination of SESN2, thereby inhibiting SESN2-interaction with the GATOR2 complex. Does not cleave 'Lys-48'-linked polyubiquitin chains.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384701.1">
        <property type="nucleotide sequence ID" value="XM_027528900.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384702.1">
        <property type="nucleotide sequence ID" value="XM_027528901.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027384703.1">
        <property type="nucleotide sequence ID" value="XM_027528902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2I6H7"/>
    <dbReference type="SMR" id="A0A4W2I6H7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001535.1">
        <property type="protein sequence ID" value="ENSBIXP00000031285.1"/>
        <property type="gene ID" value="ENSBIXG00000013529.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050485.1">
        <property type="protein sequence ID" value="ENSBIXP00005040952.1"/>
        <property type="gene ID" value="ENSBIXG00005023795.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884355"/>
    <dbReference type="CTD" id="57559"/>
    <dbReference type="GeneTree" id="ENSGT00940000153710"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08066">
        <property type="entry name" value="MPN_AMSH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000012">
        <property type="entry name" value="AMSH-like protease isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000010">
        <property type="entry name" value="AMSH-like protease isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044098">
        <property type="entry name" value="STAMBP/STALP-like_MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947:SF7">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="15">
        <location>
            <begin position="269"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="126"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="436" mass="49552" checksum="988316E176414CD4" modified="2019-09-18" version="1">MDQPLTVNSLKKLAAMPDHTDVSLTPEERVRALSKLGGNITINEDITPRRYFRSGVEMERMASVYLEEGNLESAFVLYNKFITLFVEKLPNHRDYQQCAVPEKQDIMKKLKEIAFPRTDELKKDLLKKYNVEYQEYLQSKNKYKAEILKKLEHQRLIEAERKRIAQMRQQQLESEQFSIFEDQLKKQELARSQLRSQETPALSEQIDGSALSCFSTHQNSSLLNVLADQSSKSDATSYAGHSPPVNRALKPAASLSAVQNLVVEGLRCVVLSRDLCHRFLLLAESNTVRGIETCGILCGKLTHNEFTITHLIVPKQSAGPDYCDVENVEELFSVQDQHDLLTLGWIHTHPTQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHKDTGIFRLTDAGMLEVSACKKKGFHPHTKDPRLFSVCRHVLVKDIKIIVLDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IFB1</accession>
    <name>A0A4W2IFB1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hemoglobin subunit theta-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC113883300</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in oxygen transport from the lung to the various peripheral tissues.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of two alpha chains and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the globin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027382721.1">
        <property type="nucleotide sequence ID" value="XM_027526920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IFB1"/>
    <dbReference type="SMR" id="A0A4W2IFB1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014427.1">
        <property type="protein sequence ID" value="ENSBIXP00000001246.1"/>
        <property type="gene ID" value="ENSBIXG00000028763.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000014432.1">
        <property type="protein sequence ID" value="ENSBIXP00000027123.1"/>
        <property type="gene ID" value="ENSBIXG00000028763.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000014442.1">
        <property type="protein sequence ID" value="ENSBIXP00000001244.1"/>
        <property type="gene ID" value="ENSBIXG00000028763.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038472.1">
        <property type="protein sequence ID" value="ENSBIXP00005023577.1"/>
        <property type="gene ID" value="ENSBIXG00005026352.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038492.1">
        <property type="protein sequence ID" value="ENSBIXP00005042790.1"/>
        <property type="gene ID" value="ENSBIXG00005026352.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005051969.1">
        <property type="protein sequence ID" value="ENSBIXP00005042800.1"/>
        <property type="gene ID" value="ENSBIXG00005026352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883300"/>
    <dbReference type="GeneTree" id="ENSGT00940000154590"/>
    <dbReference type="OMA" id="MFTSFPT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031720">
        <property type="term" value="F:haptoglobin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043177">
        <property type="term" value="F:organic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08927">
        <property type="entry name" value="Hb-alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000002">
        <property type="entry name" value="Hemoglobin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002338">
        <property type="entry name" value="Hemoglobin_a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002339">
        <property type="entry name" value="Hemoglobin_pi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442:SF48">
        <property type="entry name" value="HEMOGLOBIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00612">
        <property type="entry name" value="ALPHAHAEM"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00815">
        <property type="entry name" value="PIHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="10">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15184" checksum="6D20CADDA05C0DC5" modified="2019-09-18" version="1">MVLSAADKGNVKAAWGKVGGHAAEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGAKVAAALTKAVEHLDDLPGALSELSDLHAHKLRVDPVNFKLLSHSLLVTLASHLPSDFTPAVHASLDKFLANVSTVLTSKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IGI0</accession>
    <name>A0A4W2IGI0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2IGI0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040444.1">
        <property type="protein sequence ID" value="ENSBIXP00000042544.1"/>
        <property type="gene ID" value="ENSBIXG00000026546.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039489.1">
        <property type="protein sequence ID" value="ENSBIXP00005043260.1"/>
        <property type="gene ID" value="ENSBIXG00005002480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="54"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="380" mass="42986" checksum="F075584021106F76" modified="2019-09-18" version="1">MITHLEPDILECEVKWVLGSISTNKASGGDGIPVELFQILKDDAVKVLHSICQLIWKTQQWPQDWKRSVFIPIPKKGNTKEYSNYCTIALISHASKVMLKILQARLQGYMNYELPDVQAGFKRGRGTRDQIANFRWIIKKATELQKNIYFCFIDYAKAFDCVVHNILWKILKEMGIADHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCTLSPCLFNLYAEYIMRNAGLKEAQAGIKIAGRNINNLRYADDTTLMAESEEEPKSLLMKVKEESEKVGLKLNIQKTKIMASGPITSWQIDRETVETVSDFIYLGSKITADGDCSHEIKGHLLLGRKIMTNLDSILKSRDITLPTKVRLVKAMVFPVVIYGLRVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2III5</accession>
    <name>A0A4W2III5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein kinase 40</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">STK40</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be a negative regulator of NF-kappa-B and p53-mediated gene transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027393612.1">
        <property type="nucleotide sequence ID" value="XM_027537811.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393613.1">
        <property type="nucleotide sequence ID" value="XM_027537812.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393614.1">
        <property type="nucleotide sequence ID" value="XM_027537813.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027393615.1">
        <property type="nucleotide sequence ID" value="XM_027537814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2III5"/>
    <dbReference type="SMR" id="A0A4W2III5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038349.1">
        <property type="protein sequence ID" value="ENSBIXP00000041470.1"/>
        <property type="gene ID" value="ENSBIXG00000002025.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052913.1">
        <property type="protein sequence ID" value="ENSBIXP00005043957.1"/>
        <property type="gene ID" value="ENSBIXG00005027735.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113890133"/>
    <dbReference type="CTD" id="83931"/>
    <dbReference type="GeneTree" id="ENSGT00950000182986"/>
    <dbReference type="OMA" id="HGIFKDR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13974">
        <property type="entry name" value="STKc_SHIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000269">
        <property type="entry name" value="Serine/threonine-protein kinase 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024236">
        <property type="entry name" value="Ser/Thr_kinase_40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF16">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="35"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="436" mass="48929" checksum="56DDF51AD14695AF" modified="2019-09-18" version="1">MKRRASDRGAGETSARAKALGSGISGNNAKRAGPFILGPRLGNSPVPSIVQCLARKDGTDDFYQLKILTLEERGDQGIESQEERQGKMLLHTEYSLLSLLHTQDGVVHHHGLFQDRTCEVVEDAESNRMVKKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHQKNVVHRDLKLGNMVLSKRTHRITITNFCLGKHLVSEGDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLAAADVLEALSSIIASWQSLSSLSGPLQVVPDIDDQMSNADSSQEAKVTEECSQYEFENYMRQQLLLAEEKSSVHEARSWVPKRQSGAGVPPVRRLGHDAQPVNPLDAAILAQRYLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNN0</accession>
    <name>A0A4W2BNN0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Ras-related protein Rab-30</fullName>
            <ecNumber evidence="6">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RAB30</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB30 is required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins. Facilitates lipid homeostasis during fasting by regulating hepatic protein and lipid trafficking in a PPAR-alpha-dependent manner. Promotes autophagosome biogenesis during bacterial infection such as group A Streptococcus infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="22">Autolysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Autophagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Golgi apparatus</location>
            <location evidence="20">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027388031.1">
        <property type="nucleotide sequence ID" value="XM_027532230.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388032.1">
        <property type="nucleotide sequence ID" value="XM_027532231.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388033.1">
        <property type="nucleotide sequence ID" value="XM_027532232.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027388034.1">
        <property type="nucleotide sequence ID" value="XM_027532233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNN0"/>
    <dbReference type="SMR" id="A0A4W2BNN0"/>
    <dbReference type="STRING" id="30522.A0A4W2BNN0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013636.1">
        <property type="protein sequence ID" value="ENSBIXP00000001602.1"/>
        <property type="gene ID" value="ENSBIXG00000008143.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005047881.1">
        <property type="protein sequence ID" value="ENSBIXP00005037680.1"/>
        <property type="gene ID" value="ENSBIXG00005019596.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886207"/>
    <dbReference type="CTD" id="27314"/>
    <dbReference type="GeneTree" id="ENSGT00940000156875"/>
    <dbReference type="OMA" id="VYDVSCQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120281">
        <property type="term" value="C:autolysosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031985">
        <property type="term" value="C:Golgi cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04114">
        <property type="entry name" value="Rab30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000440">
        <property type="entry name" value="Ras-related protein Rab-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041820">
        <property type="entry name" value="Rab30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0636">Prenylation</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00067826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="203" mass="23058" checksum="935E56DBD48DD7C2" modified="2019-09-18" version="1">MSMEDYDFLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEINGEKVKLQIWDTAGQERFRSITQSYYRSANALILTYDITCEESFRCLPEWLREIEQYASNKVITVLVGNKIDLAERREVSQQRAEEFSEAQDMYYLETSAKESDNVEKLFLDLACRLISEARQNTLVNNVSSPLPGEGKSISYLTCCNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BPW5</accession>
    <name>A0A4W2BPW5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear FMR1 interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NUFIP2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027374785.1">
        <property type="nucleotide sequence ID" value="XM_027518984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BPW5"/>
    <dbReference type="STRING" id="30522.A0A4W2BPW5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014757.1">
        <property type="protein sequence ID" value="ENSBIXP00000001106.1"/>
        <property type="gene ID" value="ENSBIXG00000028944.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019605.1">
        <property type="protein sequence ID" value="ENSBIXP00005034535.1"/>
        <property type="gene ID" value="ENSBIXG00005002302.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878123"/>
    <dbReference type="CTD" id="57532"/>
    <dbReference type="GeneTree" id="ENSGT00440000038328"/>
    <dbReference type="OMA" id="NEQKGNR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032747">
        <property type="entry name" value="NUFIP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28333:SF2">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28333">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15293">
        <property type="entry name" value="NUFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="697" mass="76281" checksum="128C4E2D31832E82" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BUP4</accession>
    <name>A0A4W2BUP4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BUP4"/>
    <dbReference type="SMR" id="A0A4W2BUP4"/>
    <dbReference type="STRING" id="30522.A0A4W2BUP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008931.1">
        <property type="protein sequence ID" value="ENSBIXP00000003901.1"/>
        <property type="gene ID" value="ENSBIXG00000010292.1"/>
    </dbReference>
    <dbReference type="OMA" id="PWIYSNM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="165"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="274"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="515" mass="57192" checksum="BEAD999938993568" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BVX6</accession>
    <name>A0A4W2BVX6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BVX6"/>
    <dbReference type="STRING" id="30522.A0A4W2BVX6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009928.1">
        <property type="protein sequence ID" value="ENSBIXP00000003398.1"/>
        <property type="gene ID" value="ENSBIXG00000009809.1"/>
    </dbReference>
    <dbReference type="OMA" id="KPMISCK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000003">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
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        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="170" mass="19654" checksum="A24CB563545B53D0" modified="2019-09-18" version="1">MQIKRCKSSCSLKNTRETAQAIKGIHIRKATKYLKDVTLKKQCVPFRRYNGGVGRCAQAKQWGWTQGRWRKKSAEFLLHVLKNAETNAELKGLDVDSLVIEHIQVNKAPKMRRGTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEAAQKKKISQKKLKKQKLMARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BWT3</accession>
    <name>A0A4W2BWT3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BWT3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009686.1">
        <property type="protein sequence ID" value="ENSBIXP00000003524.1"/>
        <property type="gene ID" value="ENSBIXG00000009932.1"/>
    </dbReference>
    <dbReference type="OMA" id="CKDIQPV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="89"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="478" mass="54582" checksum="FC0C5B9A5A8FE09D" modified="2019-09-18" version="1">MVYTDLTEAEDIKKRWQEYTELYKKDLHDPDNHDGVITDLEPDILECEVKWALESITTNKASGGDGIPVELLQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHGSKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANICWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWEILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGLRQGCILSPCLFNLHVEHIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESEKVGLKLNIQKMKIMASGPITSWEIDGETVETVSDFIFGGSKITADGDCNHEIKRRLLLGRKVMSNLDSIFKSRNITLPTKVRLVKAMVFPVVMYGRESWTVKKAERRRIAAFSTVVLEKTLESPLDCKDIQPVHSEGDQPWEFFGRNDAKAETPVLWPPHAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BXB5</accession>
    <name>A0A4W2BXB5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BXB5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009301.1">
        <property type="protein sequence ID" value="ENSBIXP00000003724.1"/>
        <property type="gene ID" value="ENSBIXG00000010135.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000318">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000357">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="125"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="306"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="479" mass="53887" checksum="EB87D22AEE19E478" modified="2019-09-18" version="1">MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDANGAVVKTNANAEKADEEEKEDRAAQSLLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPLMLAEPPQNLIAQSQSGTGKTAAFVLAMLSQVEPANRHPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEHIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNIIKLKREEETLDTIKQYYVLCNSRDEKFQALCNIYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMVVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZ57</accession>
    <name>A0A4W2BZ57_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carnitine O-palmitoyltransferase 1, muscle isoform</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carnitine O-palmitoyltransferase I, muscle isoform</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Carnitine palmitoyltransferase 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">CPT1B</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027396190.1">
        <property type="nucleotide sequence ID" value="XM_027540389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BZ57"/>
    <dbReference type="SMR" id="A0A4W2BZ57"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007383.1">
        <property type="protein sequence ID" value="ENSBIXP00000004648.1"/>
        <property type="gene ID" value="ENSBIXG00000010968.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005008721.1">
        <property type="protein sequence ID" value="ENSBIXP00005004230.1"/>
        <property type="gene ID" value="ENSBIXG00005000825.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892053"/>
    <dbReference type="CTD" id="1375"/>
    <dbReference type="GeneTree" id="ENSGT01150000286917"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015909">
        <property type="term" value="P:long-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF69">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="24">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="23">
        <location>
            <begin position="175"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="771" mass="88512" checksum="67FAB2B2531EDA85" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2W0</accession>
    <name>A0A4W2C2W0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C2W0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004424.1">
        <property type="protein sequence ID" value="ENSBIXP00000006100.1"/>
        <property type="gene ID" value="ENSBIXG00000006383.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="336" mass="38824" checksum="134300D186CD4887" modified="2019-09-18" version="1">MEKAREFQKNIYFCFIDYAKAFDCVDHDKLWKILKEMGIPDHLTCLLRILYAGQEVTVRTGHGTTDWFQIEKGVCQGCILSPCLFNFYAEYIMRNAGLEEAQAGIKTAGRNINNLRYADDTTLMAESEEELKSLLIKVKEESEKVGLKLNIQKLKIMAFGPITSWEIEGETVETVSDFIFWGSKITADGNCSHEIKRRLLLGRKVMTNLDSILKSRDITLPTKVRLVKAVFPVVMYGCESWTVKKAERQRIDDFELWCWRRLLRVPWTSRRSNQSILKEISPGCSLEGLLLKLKLQYFGHLVRRVDSLEKTLMLGGAGGRRRRVRQRMRWLDGITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C431</accession>
    <name>A0A4W2C431_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex protein 3 homolog</fullName>
            <shortName evidence="5">NOC3 protein homolog</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C431"/>
    <dbReference type="STRING" id="30522.A0A4W2C431"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004018.1">
        <property type="protein sequence ID" value="ENSBIXP00000006298.1"/>
        <property type="gene ID" value="ENSBIXG00000001173.1"/>
    </dbReference>
    <dbReference type="OMA" id="HYCPQVR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="9">
        <location>
            <begin position="213"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="8">
        <location>
            <begin position="554"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="449"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="799" mass="92304" checksum="B906974B45E13DAD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C472</accession>
    <name>A0A4W2C472_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Laminin subunit beta 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LAMB3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C472"/>
    <dbReference type="STRING" id="30522.A0A4W2C472"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001616.1">
        <property type="protein sequence ID" value="ENSBIXP00000007542.1"/>
        <property type="gene ID" value="ENSBIXG00000008025.1"/>
    </dbReference>
    <dbReference type="OMA" id="NKREQFE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043256">
        <property type="term" value="C:laminin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070831">
        <property type="term" value="P:basement membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000084">
        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000101">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000440">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000073">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000001">
        <property type="entry name" value="Laminin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000166">
        <property type="entry name" value="laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF268">
        <property type="entry name" value="LAMININ SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23219">
        <property type="entry name" value="LAMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021264391" evidence="15">
        <location>
            <begin position="18"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="17">
        <location>
            <begin position="22"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="316"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="379"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="431"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="481"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="534"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="839"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="949"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1043"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1172" mass="128876" checksum="F80A5FE687B0A7F5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C4C8</accession>
    <name>A0A4W2C4C8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BAR/IMD domain-containing adapter protein 2-like 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Planar intestinal- and kidney-specific BAR domain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BAIAP2L2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Phosphoinositides-binding protein that induces the formation of planar or gently curved membrane structures. Binds to phosphoinositides, including to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) headgroups. There seems to be no clear preference for a specific phosphoinositide.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027396618.1">
        <property type="nucleotide sequence ID" value="XM_027540817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C4C8"/>
    <dbReference type="SMR" id="A0A4W2C4C8"/>
    <dbReference type="STRING" id="30522.A0A4W2C4C8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003370.1">
        <property type="protein sequence ID" value="ENSBIXP00000006630.1"/>
        <property type="gene ID" value="ENSBIXG00000012728.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052244.1">
        <property type="protein sequence ID" value="ENSBIXP00005024147.1"/>
        <property type="gene ID" value="ENSBIXG00005026798.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892235"/>
    <dbReference type="CTD" id="80115"/>
    <dbReference type="GeneTree" id="ENSGT00940000153560"/>
    <dbReference type="OMA" id="QNLMEHF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:actin crosslink formation"/>
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    <sequence length="528" mass="59048" checksum="0679A5A5C1138215" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <text evidence="5">Belongs to the RIPOR family.</text>
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    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="13" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1009">Hearing</keyword>
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    <keyword evidence="10" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0734">Signal transduction inhibitor</keyword>
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        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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        <location>
            <begin position="82"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF15903"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1050" mass="116293" checksum="6C08EE1C606DE901" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C4V3</accession>
    <name>A0A4W2C4V3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MRS2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Magnesium transporter that mediates the influx of magnesium into the mitochondrial matrix and regulates magnesium metabolism. Also permeable to calcium, sodium and potassium ions. Required for normal expression of the mitochondrial respiratory complex I subunits. May play a role in maintaining the inner mitochondrial membrane potential.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381172.1">
        <property type="nucleotide sequence ID" value="XM_027525371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C4V3"/>
    <dbReference type="STRING" id="30522.A0A4W2C4V3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001131.1">
        <property type="protein sequence ID" value="ENSBIXP00000007788.1"/>
        <property type="gene ID" value="ENSBIXG00000013676.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005007905.1">
        <property type="protein sequence ID" value="ENSBIXP00005030430.1"/>
        <property type="gene ID" value="ENSBIXG00005010580.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882168"/>
    <dbReference type="CTD" id="57380"/>
    <dbReference type="GeneTree" id="ENSGT00390000009988"/>
    <dbReference type="OMA" id="TRNNCII"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000007">
        <property type="entry name" value="Magnesium transporter MRS2 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000003">
        <property type="entry name" value="Magnesium transporter MRS2 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF0">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="443" mass="50503" checksum="B5D97555F0E4EBDF" modified="2019-09-18" version="1">MECLRSLAQLLPRAVGLPRRTLCALALGLTPGTRPVVAYGRATELLGRRRAAPLCWSRRLPVTGEIYRNRTSDVSQATLASVSPVFTVTKFDKEGKVTSFERKKTDLYQELGLQARDLRFQHLMSITTRNNRIIMRMEYLKAVITPECLLILDYRNLNLEQWLFRELPSQLAGEGQLVTYPLPFEFRAIEALLQYRINTLQGKLSVLQPLILETLDALVDPKHSSVDRSKLHILLQNGKSLSELETDIKIFKESILEILDEEELLEDLCLTKWSDPQVFEKSSAGIDHAEEMELLLENYYRLAEDLSNAARELRALIDDSQSIIFINLDSHRNVMMRLNLQLTMGTFSLSLFGLMGVAFGMNLESSLEEDHRVFWLITGIMFMGSGLIWRRLLSFLGRQLEAPLPPLMASLPKKTLQADRRLEMKHSFRPDGLGSSRGILTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5A8</accession>
    <name>A0A4W2C5A8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NUP85</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
            <location evidence="1 10">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C5A8"/>
    <dbReference type="SMR" id="A0A4W2C5A8"/>
    <dbReference type="STRING" id="30522.A0A4W2C5A8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003118.1">
        <property type="protein sequence ID" value="ENSBIXP00000006767.1"/>
        <property type="gene ID" value="ENSBIXG00000012837.1"/>
    </dbReference>
    <dbReference type="OMA" id="ELMEWLN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8 10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="656" mass="74710" checksum="D5F1A80D44C828B9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5I1</accession>
    <name>A0A4W2C5I1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">WD repeat-containing protein 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Bromodomain and WD repeat-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">WDR11</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the Hedgehog (Hh) signaling pathway, is essential for normal ciliogenesis. Regulates the proteolytic processing of GLI3 and cooperates with the transcription factor EMX1 in the induction of downstream Hh pathway gene expression and gonadotropin-releasing hormone production. WDR11 complex facilitates the tethering of Adaptor protein-1 complex (AP-1)-derived vesicles. WDR11 complex acts together with TBC1D23 to facilitate the golgin-mediated capture of vesicles generated using AP-1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
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            <location evidence="3">Cilium axoneme</location>
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        <subcellularLocation>
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        <subcellularLocation>
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            <location evidence="5">trans-Golgi network</location>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
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    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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    <feature type="domain" description="WDR11 first beta-propeller" evidence="18">
        <location>
            <begin position="21"/>
            <end position="319"/>
        </location>
    </feature>
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        <location>
            <begin position="483"/>
            <end position="824"/>
        </location>
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        <location>
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        <source>
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        <source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="1222" mass="136058" checksum="2F583B270307B5AB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C6I7</accession>
    <name>A0A4W2C6I7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113881455</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="319" mass="35931" checksum="0E76D1792775B341" modified="2019-09-18" version="1">MERANRTLVTQFIFMRFSNSPLLQLLFFSLFLLVYLLSLVGNGLIVLIVALDRRLHTPMYFFICNLSLVELWYTTVTVPKMLANFLSSQGVISVPSCITQYYFFFSLAATELFFLTTMAYDRYAAICRPLHYPLLLSPQTCGTLAGVCWCVGFLCPMFPSFLLTQISFCTPNQINHFFCDADQIFRLSCTDTYAIQAVGYAFSTVIILGALVFTMASYAHILATILAMASAAAQRKAFSTCTAHLSVVTIYFGTLIFMYVCPAVRYESSINKIVAIFYSVITPLLNPLIYTLRNKDVKEALKVLVSRMKKVYHSSGETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7A9</accession>
    <name>A0A4W2C7A9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C7A9"/>
    <dbReference type="STRING" id="30522.A0A4W2C7A9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016504.1">
        <property type="protein sequence ID" value="ENSBIXP00000008706.1"/>
        <property type="gene ID" value="ENSBIXG00000001739.1"/>
    </dbReference>
    <dbReference type="OMA" id="CELFAVN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="497"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="704" mass="81484" checksum="773FED9900C95849" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C964</accession>
    <name>A0A4W2C964_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Signal peptide peptidase like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SPPL3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027422357.1">
        <property type="nucleotide sequence ID" value="XM_027566556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C964"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016052.1">
        <property type="protein sequence ID" value="ENSBIXP00000008393.1"/>
        <property type="gene ID" value="ENSBIXG00000014242.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042857.1">
        <property type="protein sequence ID" value="ENSBIXP00005026548.1"/>
        <property type="gene ID" value="ENSBIXG00005005011.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907414"/>
    <dbReference type="CTD" id="121665"/>
    <dbReference type="GeneTree" id="ENSGT00940000158101"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF22">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="384" mass="42251" checksum="57DBF3363734CF33" modified="2019-09-18" version="1">MAEQTYSWAYSLVDSSQVSTFLISILLIVYGSFRSLNMDFENQDKEKDSNSSSGSFNGNSTNNSIQTIDSTQALFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFTAAELLSFSLSVMLVLIWVLTGHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYIFNSNVMVKVATQPADNPLDVLSRKLHLGPNVGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPGLLLCFVLRYDNYKKQASGDSCGASGPANISGRMQKVSYFHCTLIGYFVGLLTATVASRIHRAAQPALLYLVPFTLLPLLTMAYLKGDLRRMWSEPFHSKSSSSRFLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CC50</accession>
    <name>A0A4W2CC50_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nipped-B protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NIPBL</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin. Plays a role in cohesin loading at sites of DNA damage. Its recruitment to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitment to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner. Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375911.1">
        <property type="nucleotide sequence ID" value="XM_027520110.1"/>
    </dbReference>
    <dbReference type="STRING" id="30522.A0A4W2CC50"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044666.1">
        <property type="protein sequence ID" value="ENSBIXP00000010488.1"/>
        <property type="gene ID" value="ENSBIXG00000015967.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013002.1">
        <property type="protein sequence ID" value="ENSBIXP00005002066.1"/>
        <property type="gene ID" value="ENSBIXG00005008477.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878926"/>
    <dbReference type="CTD" id="25836"/>
    <dbReference type="GeneTree" id="ENSGT00390000010427"/>
    <dbReference type="OMA" id="KQNENRM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070087">
        <property type="term" value="F:chromo shadow domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036033">
        <property type="term" value="F:mediator complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071481">
        <property type="term" value="P:cellular response to X-ray"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050890">
        <property type="term" value="P:cognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042471">
        <property type="term" value="P:ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048557">
        <property type="term" value="P:embryonic digestive tract morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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        <name evidence="15" type="primary">LOC113875703</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="13">Component of the mitochondrial respiratory complex IV (CIV, also named cytochrome c oxidase complex), the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The CIV complex is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Acts as an assembly factor that specifically drives the homodimerization of CIV complexes, mediating the formation of mitochondrial respiratory supercomplexes (respirasomes) containing two CIV: supercomplxes with two molecules of CIV show improved activity. Despite being highly expressed in brown adipose tissue, not required for thermogenesis.</text>
    </comment>
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        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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        <text evidence="3">Belongs to the cytochrome c oxidase VIIa family.</text>
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    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
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            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="80" mass="9063" checksum="A33FE89A867472A0" modified="2019-09-18" version="1">MRALRVSQALVRSFSSTARNRFENRVAEKQKLFQEDNGLPVHLKGGATDNILYRVTMTLCLGGTLYSLYCLGWASFPHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGM1</accession>
    <name>A0A4W2CGM1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGM1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045457.1">
        <property type="protein sequence ID" value="ENSBIXP00000012210.1"/>
        <property type="gene ID" value="ENSBIXG00000017286.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="185"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="472" mass="54315" checksum="35A6EFF6D9E276A7" modified="2019-09-18" version="1">MCRIILTHSKSKMAVWGGLTNSCERREVKSKGEKERYKHLNAEFQRIARRDKKSFLSDQCKEIEENNRMGMTRDLFKKIRDTKGTFHAKMGSRKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDNHDGVITHLEPDILECEVKWALGSITTNKASGGDGILVELFQILKDDAVKVLHSICQQMWKTQQWPQDWKRSVFIPIPKKGNAKECSNYCTIALISHTSKVMLKILQPRFQQYVNCELPDVQAGFRKGRGTRDQIANIHWIMEKAREFQKNTYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLICLLRNLYAGQEATVRTGHGTTDWFQIGKGVCQDCILSPCLFNLYAEYIMRNIGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKVESKKVGLKLNIQKTIMASSPITSWQIDGETVETVSDFSFLGSKITADDDCSHEIKDAYSLEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHJ5</accession>
    <name>A0A4W2CHJ5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CHJ5"/>
    <dbReference type="SMR" id="A0A4W2CHJ5"/>
    <dbReference type="STRING" id="30522.A0A4W2CHJ5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022534.1">
        <property type="protein sequence ID" value="ENSBIXP00000012558.1"/>
        <property type="gene ID" value="ENSBIXG00000002977.1"/>
    </dbReference>
    <dbReference type="OMA" id="EEHKYTR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061975">
        <property type="term" value="P:articular cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000167">
        <property type="entry name" value="epiphycan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043547">
        <property type="entry name" value="Mimecan/Epiphycan/Opticin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46269">
        <property type="entry name" value="EPIPHYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46269:SF4">
        <property type="entry name" value="OPTICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021204511" evidence="10">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="321" mass="35731" checksum="FB87F0CEEE7E0EC3" modified="2019-09-18" version="1">MKLLALLSLLILMLQEARTASLSEEREGDPYAILHLGDYVLSLDNYDEVIDPSNYDELIDYGDQLPQVKGTSLASLTRTRFTQSTEAARTLPSNPTTARPPTLGLLAAPANHGLPTCLICVCLGSSVYCDDADLENIPPLPQTTAYLYARFNRISHIRAGDFKGLTKLKRIDLSGNSISSIDDKALRLLPALRDLILPENKLVALPTLPTSIEVLDVRMNRLQSSGIQPEAFRALEKLQFLYLADNLLDAIPPSLPLSLRSLHLQNNMIETMQRDAFCDAEEHRHTRRPLEDIRLDGNPINLSLFPSAYFCLPRLPTGRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CI12</accession>
    <name>A0A4W2CI12_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase FANCL</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase FANCL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">FANCL</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CI12"/>
    <dbReference type="STRING" id="30522.A0A4W2CI12"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020972.1">
        <property type="protein sequence ID" value="ENSBIXP00000011546.1"/>
        <property type="gene ID" value="ENSBIXG00000016788.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046478.1">
        <property type="protein sequence ID" value="ENSBIXP00005013054.1"/>
        <property type="gene ID" value="ENSBIXG00005017270.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005537"/>
    <dbReference type="OMA" id="NRPFHAK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23832">
        <property type="entry name" value="DRWD-C_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23831">
        <property type="entry name" value="DRWD-N_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23786">
        <property type="entry name" value="ELF_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16490">
        <property type="entry name" value="RING-CH-C4HC3_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000081">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000221">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.20">
        <property type="entry name" value="RWD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026848">
        <property type="entry name" value="Fancl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026850">
        <property type="entry name" value="FANCL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043898">
        <property type="entry name" value="FANCL_d2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044037">
        <property type="entry name" value="FANCL_d3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043003">
        <property type="entry name" value="FANCL_d3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019162">
        <property type="entry name" value="FancL_WD-rpt_cont_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN PHF9 FANCONI ANEMIA GROUP L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11793">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09765">
        <property type="entry name" value="FANCL_d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18890">
        <property type="entry name" value="FANCL_d2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18891">
        <property type="entry name" value="FANCL_d3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="309"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="377" mass="43010" checksum="B82CEBDEFA9ED48F" modified="2019-09-18" version="1">MAETEATLLRQFPLFLPQNRSKTVYEGFISAQGRDFHLRLLLPKDLQLRNARLLCSWQLKTALNGYHHIVQQRMQQSPDLVSFMMELKMVLEAALKNKKEPYVLPSPPKFYSSLIGEIRTLGWDKLVFVDPCFSTIKLKADVSGREHLITVKLKAKYPAESPDCVVDFPVPFSVSWTPQVNSSSLISIYTQFLAALESLKTFWDVMDEIDEKTWVLEPEKPTRSATARRIVLGNNVSINIEVDPRHPTMLPECCFLGADHVVKPLGIRLSRNIHLWDPENSLLQNLKDVLEIDFPARAILEKSDFSMDCGICYAYQLDGAIPDQVCDNSQCGQSFHYICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CID7</accession>
    <name>A0A4W2CID7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ADAM metallopeptidase domain 23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ADAM23</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CID7"/>
    <dbReference type="STRING" id="30522.A0A4W2CID7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045793.1">
        <property type="protein sequence ID" value="ENSBIXP00000012858.1"/>
        <property type="gene ID" value="ENSBIXG00000017916.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000147">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000014">
        <property type="entry name" value="disintegrin and metalloproteinase domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF13">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="795"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="17">
        <location>
            <begin position="300"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="16">
        <location>
            <begin position="503"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="733"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="833" mass="92173" checksum="E8CAB586D3913048" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKZ4</accession>
    <name>A0A4W2CKZ4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904793</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027417683.1">
        <property type="nucleotide sequence ID" value="XM_027561882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CKZ4"/>
    <dbReference type="SMR" id="A0A4W2CKZ4"/>
    <dbReference type="STRING" id="30522.A0A4W2CKZ4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045589.1">
        <property type="protein sequence ID" value="ENSBIXP00000012443.1"/>
        <property type="gene ID" value="ENSBIXG00000005966.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032541.1">
        <property type="protein sequence ID" value="ENSBIXP00005019602.1"/>
        <property type="gene ID" value="ENSBIXG00005022752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904793"/>
    <dbReference type="GeneTree" id="ENSGT01150000286940"/>
    <dbReference type="OMA" id="RCRFQFY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF397">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="312" mass="35411" checksum="81578D4389FFD4EC" modified="2019-09-18" version="1">MSISNITVFMPSVFMLIGIPGLESVQCWIGIPFCAMYLTAVIGNALLLIIIKSEPSLHEPMYIFLGMLGVTDIALSTSIVPKMLGIFWFHIPEIYFDSCLLQMWLIHTFEGFESGFLLAMALDRYVAICYPLRHASIFTPQLVTQIAAMVTLRATIFVTPSLVLIKHQLHFFHTKVISHCYCEHMAIVKLAAEDARVNKIYGLFVAFTVAGFDLICISLSYVQIFTTVFRLPQKEARLKAFNTCIAHICVFLQFYLLAFFSFFAHRFGSHIPPYIHILFSSIYLLVPPFLNPLVYGAKTKQIRIHVVKMFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CLK9</accession>
    <name>A0A4W2CLK9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CLK9"/>
    <dbReference type="STRING" id="30522.A0A4W2CLK9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024680.1">
        <property type="protein sequence ID" value="ENSBIXP00000014001.1"/>
        <property type="gene ID" value="ENSBIXG00000018807.1"/>
    </dbReference>
    <dbReference type="OMA" id="LMFGWAP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000010">
        <property type="entry name" value="probable cationic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF4">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="503"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
        <location>
            <begin position="538"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="611"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="630" mass="67470" checksum="6538F8E0C266562B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQU2</accession>
    <name>A0A4W2CQU2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQU2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043742.1">
        <property type="protein sequence ID" value="ENSBIXP00000008689.1"/>
        <property type="gene ID" value="ENSBIXG00000014464.1"/>
    </dbReference>
    <dbReference type="OMA" id="LISNCIC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047123">
        <property type="entry name" value="MYADM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068:SF3">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER MYADM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021503434" description="MARVEL domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="138"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="315" mass="36296" checksum="EE1759FC541338E1" modified="2019-09-18" version="1">MGYFLRTLQLLSTCVAISLVGSLDSWTVPTSTWSFVILCFCFVVTFIILIIESLALHYCFPFSWGDFLLCHACYLALFCLLVSIIYPATYVQFLFYTPSWDHAIAATAGLLSCIMCVLYAIDMAYMWKRYKLKNIPCYVHTLPGLLKILESAVACVIFVFISNTSLYLHQPALEWCVAVYSICFVQVVVAMLLKLRGWEKRLPLRLPTFHRVGTWFSFLLYVIAMVLWPLYQFNEKLGGQPHWYRDMSCVDELTNYGCVWDQQLAVAILTAINLVIYMADLVYWTRQVSVGLRTSPVYFIPSTKQRWSSQNSIYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CV22</accession>
    <name>A0A4W2CV22_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CV22"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027386.1">
        <property type="protein sequence ID" value="ENSBIXP00000015783.1"/>
        <property type="gene ID" value="ENSBIXG00000020151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="32"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="383" mass="43751" checksum="EEF0C4FE6174670E" modified="2019-09-18" version="1">MNKASGGDRIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKSSVFIPIPKKGNAKECSNYRTIALISHASKVMLKILQARLQQYVNCELPDVQAGFRKGRGTRDQIANICWIMEKAREFQKNLYFCFIDYAKAFDCVNHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGYGTTNWFQIGKGVCQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKMKIMASSPITSWEIDGETVETVSDFIFLGSKITADGDCSREIKRCLLLGRKVMTNLDSIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAECQRIDAFELWCWRRLLGPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVW6</accession>
    <name>A0A4W2CVW6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Copine-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Copine I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC113903074</name>
        <name evidence="24" type="synonym">CPNE1</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner. Exhibits calcium-dependent phospholipid binding properties. Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner. May recruit target proteins to the cell membrane in a calcium-dependent manner. May function in membrane trafficking. Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit. Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer; homodimerizes via its C2 domains. Interacts with p65/RELA (via N-terminus); this interaction induces proteolytic cleavage of p65/RELA subunit and inhibition of NF-kappa-B transcriptional activity. Interacts (via VWFA domain) with ACTB, CCDC22, MYCBP2, PPP5C, RDX and UBE2O.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027414471.1">
        <property type="nucleotide sequence ID" value="XM_027558670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CVW6"/>
    <dbReference type="STRING" id="30522.A0A4W2CVW6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030118.1">
        <property type="protein sequence ID" value="ENSBIXP00000017557.1"/>
        <property type="gene ID" value="ENSBIXG00000021571.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009447.1">
        <property type="protein sequence ID" value="ENSBIXP00005003888.1"/>
        <property type="gene ID" value="ENSBIXG00005010114.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903074"/>
    <dbReference type="GeneTree" id="ENSGT00940000162210"/>
    <dbReference type="OMA" id="SPTWITK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
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        <property type="term" value="P:cellular response to calcium ion"/>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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            <begin position="19"/>
            <end position="542"/>
        </location>
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        <location>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017557.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="542" mass="59581" checksum="D40C6FD3DED16338" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CWW3</accession>
    <name>A0A4W2CWW3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein G(s) subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Adenylate cyclase-stimulating G alpha protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC113903214</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the G-alpha family. G(s) subfamily.</text>
    </comment>
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    <dbReference type="STRING" id="30522.A0A4W2CWW3"/>
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        <property type="protein sequence ID" value="ENSBIXP00000016342.1"/>
        <property type="gene ID" value="ENSBIXG00000007608.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031698">
        <property type="term" value="F:beta-2 adrenergic receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051430">
        <property type="term" value="F:corticotropin-releasing hormone receptor 1 binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031748">
        <property type="term" value="F:D1 dopamine receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005159">
        <property type="term" value="F:insulin-like growth factor receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031852">
        <property type="term" value="F:mu-type opioid receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007191">
        <property type="term" value="P:adenylate cyclase-activating dopamine receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000367">
        <property type="entry name" value="Gprotein_alpha_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF348">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(S) SUBUNIT ALPHA ISOFORMS XLAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00443">
        <property type="entry name" value="GPROTEINAS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="291"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="457"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="610"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="678"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
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    <sequence length="156" mass="17096" checksum="C0EA5F5AEFA31D34" modified="2019-09-18" version="1">MEKLLALSLVILWLQLAKVNNQQGKQKLQTLSIQEGENFTTYCNSSSILSSLQWYKQSPGGSPVVLMILAKGGEVKTEQRLTGQFGETKQHSSLHLTAAQLSDAGTYFCAGHSALEAPAVCPQTSLWALAVTPPHLSSEQRPEIAEVMMSMKKFKF</sequence>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <dbReference type="Proteomes" id="UP000314981"/>
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</entry>
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    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <text>a CDP-1,2-diacyl-sn-glycerol + a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = a cardiolipin + CMP + H(+)</text>
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        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
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        <property type="entry name" value="CDP-OH_P_trans"/>
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        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
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        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="CDP-OH_P_transf"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="244"/>
            <end position="262"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="268"/>
            <end position="289"/>
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    </feature>
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        <location>
            <begin position="67"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00068900"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015000.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="302" mass="32789" checksum="B690EFD3AD5C2EBF" modified="2019-09-18" version="1">MLASRMARASWGALRVAAWAPGARPGKGRARGALLPPAPGCLGFLAERWWLRPAALGLRLPGASPRAHCSGAEKATPGPATGEDATVEARRGQWGPTSASNLYENPWTIPNMLSMTRIGLAPVLGYLIIEEDFNIALGVFALAGLTDLLDGFIARNWASQKSALGSALDPLADKILISILYISLTYADLIPVPLTYMIISRDVMLIAAVFYVRYRTLPTPRTLSKYFNPCYATARLKPTFISKVNTAVQLILVAASLAAPVFNYADSIYLQILWCFTAFTTAASAYSYYHYGRKTVQVIKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9K2</accession>
    <name>A0A4W2D9K2_BOBOX</name>
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        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 39</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="4">Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC40 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly.</text>
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        <text evidence="1">Belongs to the CCDC39 family.</text>
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        <source>
            <dbReference type="ARBA" id="ARBA00045182"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020974.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="941" mass="109970" checksum="0FD3CD1E9014C288" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DA85</accession>
    <name>A0A4W2DA85_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DA85"/>
    <dbReference type="SMR" id="A0A4W2DA85"/>
    <dbReference type="STRING" id="30522.A0A4W2DA85"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049788.1">
        <property type="protein sequence ID" value="ENSBIXP00000021089.1"/>
        <property type="gene ID" value="ENSBIXG00000007126.1"/>
    </dbReference>
    <dbReference type="OMA" id="DVYWFQK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026139">
        <property type="entry name" value="GOLM1/CASC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15896:SF8">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15896">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 2/GP73-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02084">
        <property type="entry name" value="GOLM1CASC4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="178"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="405" mass="45536" checksum="4209C0CD56BACCC9" modified="2019-09-18" version="1">MMGLGNGRRSMKSPPLVLAALVACIIVLGFNYWIASSRSVDLQTRIVELEGRVRRAAAERGAVEMKKNEFQGELEKQREQLDKIQSSHNFQMESVNKLYQDEKAVLVNNITTGERLIRTLQDQLKALQKSYGRLQQDVLQFQKNQTNLERKFSYDLNQCISQMKEVKEQCEERIEEVTRKGNEAMPSHDLNEQKDQSQQPRAPSESQPRPQEAGPPQAEVPEARGNVPSHSEPQTPAPSSEVAFDLKRQGEKEETKEIQVVSEEELQRDSLALPKEPGREQEVEGDRQVGGKGNGEPGELGQIPQVPAALLASQENQEAEGPERDQLVIRDGQEEGQDADEEGRNQQKLGADDDYNMDENEAESETDKQAALAGNDKNRNVLNAENQKGDTINLLDQHEKRNHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAE4</accession>
    <name>A0A4W2DAE4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">WNT9A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027404560.1">
        <property type="nucleotide sequence ID" value="XM_027548759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAE4"/>
    <dbReference type="STRING" id="30522.A0A4W2DAE4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050746.1">
        <property type="protein sequence ID" value="ENSBIXP00000022960.1"/>
        <property type="gene ID" value="ENSBIXG00000002052.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006093.1">
        <property type="protein sequence ID" value="ENSBIXP00005005581.1"/>
        <property type="gene ID" value="ENSBIXG00005006783.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113896523"/>
    <dbReference type="CTD" id="7483"/>
    <dbReference type="GeneTree" id="ENSGT00940000159618"/>
    <dbReference type="OMA" id="EAWKWGG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000002">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013303">
        <property type="entry name" value="Wnt9a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF75">
        <property type="entry name" value="PROTEIN WNT-9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01894">
        <property type="entry name" value="WNT14PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610746" description="Protein Wnt" evidence="13">
        <location>
            <begin position="30"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="258"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000022960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="365" mass="40393" checksum="7539B0AE9D59EE69" modified="2019-09-18" version="1">MLDGPLLARWLAAAFALTLLLAALRPSAAYFGLTGSEPLTILPLTLEPEAVAQAHYKACDRLKLERKQRRMCRRDPGVAETLVEAVSMSALECQYQFRFERWNCTLEGRYRASLLKRGFKETAFLYAISSAGLTHALAKACSAGRMERCTCDEAPDLENREAWQWGGCGDNLKYSSKFVKEFLGRRSSKDLRARVDFHNNLVGVKVIKAGVETTCKCHGVSGSCTVRTCWRQLAPFHEVGKRLKHKYETALKVGSTTNEATGEAGAISPPRGRAAGAGGGDPLPRTPELVHLDDSPSFCVAGRFSPGTAGRRCHREKNCESICCGRGHNTQSRVVTRPCQCQVRWCCYVECRQCTQREEVYTCKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBI9</accession>
    <name>A0A4W2DBI9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan 5-hydroxylase 2</fullName>
            <ecNumber evidence="4">1.14.16.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tryptophan 5-monooxygenase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TPH2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin</text>
            <dbReference type="Rhea" id="RHEA:16709"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58266"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with DNAJC12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027399152.1">
        <property type="nucleotide sequence ID" value="XM_027543351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBI9"/>
    <dbReference type="SMR" id="A0A4W2DBI9"/>
    <dbReference type="STRING" id="30522.A0A4W2DBI9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050035.1">
        <property type="protein sequence ID" value="ENSBIXP00000021556.1"/>
        <property type="gene ID" value="ENSBIXG00000024616.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005015221.1">
        <property type="protein sequence ID" value="ENSBIXP00005000932.1"/>
        <property type="gene ID" value="ENSBIXG00005029698.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893359"/>
    <dbReference type="CTD" id="121278"/>
    <dbReference type="GeneTree" id="ENSGT00950000182885"/>
    <dbReference type="OMA" id="VHFNPYT"/>
    <dbReference type="UniPathway" id="UPA00846">
        <property type="reaction ID" value="UER00799"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004510">
        <property type="term" value="F:tryptophan 5-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009072">
        <property type="term" value="P:aromatic amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03346">
        <property type="entry name" value="eu_TrpOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000001">
        <property type="entry name" value="tryptophan 5-hydroxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005963">
        <property type="entry name" value="Trp_5_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041904">
        <property type="entry name" value="TrpOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01270">
        <property type="entry name" value="Trp_5_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF16">
        <property type="entry name" value="TRYPTOPHAN 5-HYDROXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0408">Iron</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0724">Serotonin biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="20">
        <location>
            <begin position="66"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="19">
        <location>
            <begin position="140"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="491" mass="56052" checksum="8E29E81A908FBC96" modified="2019-09-18" version="1">MQPAMMMFSSKYWARRGLSLDSAVPEEHQLLTSLTLNKTNSGKNDDKKGNKGSSKNDTATESGKTAVVFSLKNEVGGLVKALKLFQEKHVNMIHIESRKSRRRSSEVEIFVDCECGKTEFNELIQSLKFQTTIVTLNPPENIWTEEEELEDVPWFPRKISELDRCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKHCGYREDNVPQLEDVAAFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYVRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DBU5</accession>
    <name>A0A4W2DBU5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Ceruloplasmin</fullName>
            <ecNumber evidence="13">1.11.1.27</ecNumber>
            <ecNumber evidence="4">1.11.1.9</ecNumber>
            <ecNumber evidence="5">1.16.3.1</ecNumber>
            <ecNumber evidence="15">1.16.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Cuproxidase ceruloplasmin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Ferroxidase ceruloplasmin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Glutathione peroxidase ceruloplasmin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Glutathione-dependent peroxiredoxin ceruloplasmin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">LOC113893985</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols. Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:16834"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>4 Cu(+) + O2 + 4 H(+) = 4 Cu(2+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30083"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="EC" id="1.16.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:30084"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:11150"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>4 nitric oxide + O2 + 2 H2O = 4 nitrite + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:78539"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:78540"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:62633"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Found in a complex with MPO and LTF; interacts directly with MPO and LTF, which allows Fe(3+) incorporation into LTF, activation of CP ferroxidase activity and protection of CP antioxidant properties by MPO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="13"/>
    <dbReference type="EC" id="1.11.1.9" evidence="4"/>
    <dbReference type="EC" id="1.16.3.1" evidence="5"/>
    <dbReference type="EC" id="1.16.3.4" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DBU5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039188.1">
        <property type="protein sequence ID" value="ENSBIXP00000023557.1"/>
        <property type="gene ID" value="ENSBIXG00000005361.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11021">
        <property type="entry name" value="CuRO_2_ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11022">
        <property type="entry name" value="CuRO_4_ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04225">
        <property type="entry name" value="CuRO_5_ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000015">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000028">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000033">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000037">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048236">
        <property type="entry name" value="Ceruloplasmin-like_CuRO_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF226">
        <property type="entry name" value="CERULOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 28" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021484488" description="Ceruloplasmin" evidence="28">
        <location>
            <begin position="20"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="31">
        <location>
            <begin position="92"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="29">
        <location>
            <begin position="223"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="31">
        <location>
            <begin position="802"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="30">
        <location>
            <begin position="950"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038978"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048092"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00057991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062165"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00077782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00080685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00082571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1060" mass="120696" checksum="3AE36B787E4BAA34" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DCM4</accession>
    <name>A0A4W2DCM4_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DCM4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051205.1">
        <property type="protein sequence ID" value="ENSBIXP00000023858.1"/>
        <property type="gene ID" value="ENSBIXG00000026186.1"/>
    </dbReference>
    <dbReference type="OMA" id="KYEWKEP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040311">
        <property type="entry name" value="CCDC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31663:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="234" mass="26692" checksum="92A2EB9B16FE0B7E" modified="2019-09-18" version="1">MSSMLEVPGGSRLNLTGLGYFSCHSHTVVQYYSYFFFLRMDENYNLLPHGVNFQDAIFPDTQENRRMFSSLFQFSNCSQGQQLATFSSDWEVQKDTRVIQRTQGMVVTNRLPTVLLHSMWTPSRAVSITASLELRLRNASSSFVRGDCSNQEWPSTPSALLLDLKYEWKEPTKSTLSCWKPTLPAWRKGSSTPLLPRRLELRTQCSSSLQRDCVTFLTGVFNLQCCVGFCHTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DDM8</accession>
    <name>A0A4W2DDM8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-defensin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC113902700</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Has antibacterial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the beta-defensin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027413905.1">
        <property type="nucleotide sequence ID" value="XM_027558104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DDM8"/>
    <dbReference type="STRING" id="30522.A0A4W2DDM8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029047.1">
        <property type="protein sequence ID" value="ENSBIXP00000016879.1"/>
        <property type="gene ID" value="ENSBIXG00000020962.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037426.1">
        <property type="protein sequence ID" value="ENSBIXP00005022896.1"/>
        <property type="gene ID" value="ENSBIXG00005025685.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902700"/>
    <dbReference type="GeneTree" id="ENSGT00940000166155"/>
    <dbReference type="OMA" id="GRTSCWI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050544">
        <property type="entry name" value="Beta-defensin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025933">
        <property type="entry name" value="Beta_defensin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001:SF11">
        <property type="entry name" value="BETA-DEFENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15001">
        <property type="entry name" value="BETA-DEFENSIN 123-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13841">
        <property type="entry name" value="Defensin_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4 9" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6 9" id="KW-0211">Defensin</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516445" description="Beta-defensin" evidence="9">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-defensin" evidence="10">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU231113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10880" checksum="5F6388E7F4A05991" modified="2019-09-18" version="1">MKFLLLTVFLILFQMLPVCRGRKSCWIIKGHCRKDCKSGEQVKKPCRNGDYCCVPSKTDSQPHRPTQATARKYQTFTDKMKSNALLEQIPVIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DIK5</accession>
    <name>A0A4W2DIK5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="10">EGF like repeats and discoidin domains 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EDIL3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DIK5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043019.1">
        <property type="protein sequence ID" value="ENSBIXP00000026047.1"/>
        <property type="gene ID" value="ENSBIXG00000028136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042063">
        <property type="term" value="P:gliogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000230">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000246">
        <property type="entry name" value="EGF-like repeat and discoidin I-like domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF9">
        <property type="entry name" value="EGF-LIKE REPEAT AND DISCOIDIN I-LIKE DOMAIN-CONTAINING PROTEIN 3 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021199431" evidence="7">
        <location>
            <begin position="24"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="8">
        <location>
            <begin position="148"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="8">
        <location>
            <begin position="309"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="470" mass="52717" checksum="64F62251B3945086" modified="2019-09-18" version="1">MKHSVAAWLLLGLSLSVPQFCRGDICDPNPCENGGICLPGLSDGSFSCKCPDGFTGPNCSSVVEVGPCTPNPCHNGGTCEISEAYRGDTFIGYVCKCPRGFNGIHCQHNINECEAEPCKNGGICTDLVANYSCECPGEFMGRNCQYKCSGPLGIEGGIISNQQITASSTHRALFGLQKWYPYYARLNKKGLINAWTAAENDRWPWIQINLQRKMRVTGVITQGAKRIGSPEYIKSYKIAYSNDGKTWTMYKAKGTNEDMVFHGNVDNNTPYANSFTPPIKAQYVRLYPQVCRRHCTLRMELLGCELSGCSEPLGMKSGHIQDYQITASSIFRTLNMDMFTWEPRKARLDKQGKVNAWTSGHNDQSQWLQVDLLVPTKVTGIITQGAKDFGHVQFVGSYKLAYSNDGEHWTVYQDEKQRKDKVFQGNFDNDTHRKNVIDPPIYARHIRILPWSWYGRITLRSELLGCTEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKE1</accession>
    <name>A0A4W2DKE1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SH2B adapter protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adapter protein with pleckstrin homology and Src homology 2 domains</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">SH2 and PH domain-containing adapter protein APS</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Interacts with KIT/c-KIT, SHC1, EPOR, PDGFR, VAV1 and VAV3. Interacts (via N-terminal region) with SHC1. Interacts (via the phosphorylated C-terminus) with GRB2. Interacts (via its SH2 domain) with EPOR, INSR and KIT. Interacts with GRB2 after B-cell antigen receptor stimulation. Interacts (via PH domain) with VAV3. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated); after stimulation of the receptor by its extracellular ligand and subsequent autophosphorylation of the receptor. Binds INSR, GRB2, ASB6 and CAP. Insulin stimulation leads to dissociation of CAP. Binds CBS only when SH2B2/APS has become phosphorylated. INSR binding does not depend on the phosphorylation of SH2B2/APS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH2B adapter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKE1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000334.1">
        <property type="protein sequence ID" value="ENSBIXP00000026748.1"/>
        <property type="gene ID" value="ENSBIXG00000029472.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050851">
        <property type="term" value="P:antigen receptor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01231">
        <property type="entry name" value="PH_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10411">
        <property type="entry name" value="SH2_SH2B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000008">
        <property type="entry name" value="SH2B adapter protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000187">
        <property type="entry name" value="SH2B adapter protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015012">
        <property type="entry name" value="Phe_ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036290">
        <property type="entry name" value="Phe_ZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030523">
        <property type="entry name" value="SH2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035058">
        <property type="entry name" value="SH2B2_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872">
        <property type="entry name" value="SH2B ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872:SF4">
        <property type="entry name" value="SH2B ADAPTER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08916">
        <property type="entry name" value="Phe_ZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109805">
        <property type="entry name" value="Phenylalanine zipper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="209"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="428"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="526"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="568"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="568"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="648" mass="68827" checksum="0D5C5EC1BD54583A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKL2</accession>
    <name>A0A4W2DKL2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PARG</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKL2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048964.1">
        <property type="protein sequence ID" value="ENSBIXP00000019459.1"/>
        <property type="gene ID" value="ENSBIXG00000023078.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF15">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="8">
        <location>
            <begin position="499"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="7">
        <location>
            <begin position="624"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="655"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="674"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="658"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="672"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="894" mass="102043" checksum="54FD393D7D74CD56" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DKX3</accession>
    <name>A0A4W2DKX3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DKX3"/>
    <dbReference type="STRING" id="30522.A0A4W2DKX3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014626.1">
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        <property type="gene ID" value="ENSBIXG00000028867.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:camera-type eye morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22029">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor SOX"/>
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        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF27">
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    </dbReference>
    <dbReference type="Pfam" id="PF00505">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="127"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
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            <end position="127"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <end position="404"/>
        </location>
    </feature>
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            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
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        </location>
    </feature>
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        </location>
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        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="501" mass="50056" checksum="E00C49D3B1EB159C" modified="2019-09-18" version="1">MVQQTNNAENTEALLAGESSDSGAGLELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVWSQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGNANSGSAAAAASKPGEKGDKVGGSGHGGGGGGGSHAGGGGGGGASGGGANSKPAQKKSCGSKVAGGGVGKPHAKLILAGGGGKAAAATASASSSSSSSSFAAEQAGAAALLPLGAAAAAADHHSLYKARTPGASASAAASASAGLAAPGKHPAEKKVKRVYLFGGLGASSSPVGGVGAGADPSDPLGLYEEGGAGCSPDGPSLSGRSSAASSPAAGRSPADHRSYASLRAASPAPSSAPSHASSSASSSSSSSSSSSSSSSSSGSSSSDDEFEDDLLDLNPSSNFESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPDYCTPEPEEETLSDRRSEMTREPSWKATPARTRGGEEKATNWKGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLL0</accession>
    <name>A0A4W2DLL0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Oligodendrocyte-myelin glycoprotein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Binds to RTN4R.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLL0"/>
    <dbReference type="SMR" id="A0A4W2DLL0"/>
    <dbReference type="STRING" id="30522.A0A4W2DLL0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013695.1">
        <property type="protein sequence ID" value="ENSBIXP00000027319.1"/>
        <property type="gene ID" value="ENSBIXG00000008110.1"/>
    </dbReference>
    <dbReference type="OMA" id="PDKAFDQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031102">
        <property type="term" value="P:neuron projection regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000180">
        <property type="entry name" value="Oligodendrocyte myelin glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000445">
        <property type="entry name" value="Oligodendrocyte myelin glycoprotein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051071">
        <property type="entry name" value="LRR-bact_E3_ubiq_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47114">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47114:SF2">
        <property type="entry name" value="OLIGODENDROCYTE-MYELIN GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021497636" description="Oligodendrocyte-myelin glycoprotein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="15">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="440" mass="49446" checksum="4943916619D10B9C" modified="2019-09-18" version="1">MEYQILKMSPSLFILLFLTPGILCICPLQCICTERHRHVDCSGRNLTTLPSGLQENIIHLNLSYNHFTDLHNQLTQYTNLRTLDISNNRLESLPAQLPRSLWNMSAANNNIKLLEKSDTAYQWNLKYLDVSKNMLEKVVLIKNTLRSLEVLNLSSNKLWTVPTNMPSKLHTVDLSNNSLTQILPGTLINLTNLTHLYLHNNKFTFIPDQAFDQLFQLQEITLYNNRWSCDHHQNITYLLKWMMETKAHVIGTPCSSQISSLKEHSIYPTPSGFTSSLFTVSGMQTVDTINSLSTVTQSKVTKIPKQYRTKETTFGATLSKDTTLTSTDKAFVPYPEDTSTETINSREAAAATLTIHLQDGMVTNTSLTSSTKSSPTPMTLSITSGMPNDFSEMPQQSTTLNLRREETTTDGKTRLPSVASAWKVNASFLLMLNAVVMLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DM58</accession>
    <name>A0A4W2DM58_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Trefoil factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Intestinal trefoil factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DM58"/>
    <dbReference type="STRING" id="30522.A0A4W2DM58"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052045.1">
        <property type="protein sequence ID" value="ENSBIXP00000025206.1"/>
        <property type="gene ID" value="ENSBIXG00000027318.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030277">
        <property type="term" value="P:maintenance of gastrointestinal epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.110.10:FF:000001">
        <property type="entry name" value="Trefoil factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017994">
        <property type="entry name" value="P_trefoil_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017957">
        <property type="entry name" value="P_trefoil_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13826">
        <property type="entry name" value="INTESTINAL TREFOIL FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13826:SF16">
        <property type="entry name" value="TREFOIL FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00680">
        <property type="entry name" value="PTREFOIL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00025">
        <property type="entry name" value="P_TREFOIL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021229356" description="Trefoil factor 3" evidence="14">
        <location>
            <begin position="38"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="15">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="152"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000025206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="197" mass="21173" checksum="E5311735A8E1E2D7" modified="2019-09-18" version="1">MFSEQSGPQSQPVTMEARTFWLLVVAVLALGSSSSTGQYVGLSANQCAVPAKDRVDCGYPEVTPEQCNNRGCCFDSSIHGVPWCFKPLQEAGEPDRTHKVFQAPGSHTHLGPGWAPYATSVCWARRGFPGGTSGKEPTCSCKRHKRRGFSPRVGKIPWRRAGQPTPVCSPGESHGQRSLAGCSPGDHESDTTDHSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DNV1</accession>
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        <submittedName>
            <fullName evidence="8">Tetratricopeptide repeat and ankyrin repeat containing 1</fullName>
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        <name evidence="8" type="primary">TRANK1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSBIXP00000028131.1"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="TRANK1"/>
    </dbReference>
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        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529">
        <property type="entry name" value="MAMMARY TURMOR VIRUS RECEPTOR HOMOLOG 1, 2 MTVR1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529:SF4">
        <property type="entry name" value="TPR AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
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    </dbReference>
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        <location>
            <begin position="1007"/>
            <end position="1472"/>
        </location>
    </feature>
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        <location>
            <begin position="608"/>
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        </location>
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        <location>
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        </location>
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        <ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2925" mass="333087" checksum="1131885E9F6E7C3B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTR2</accession>
    <name>A0A4W2DTR2_BOBOX</name>
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        <submittedName>
            <fullName evidence="4">MAGE family member H1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">MAGEH1</name>
    </gene>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSBIXT00000006104.1">
        <property type="protein sequence ID" value="ENSBIXP00000029761.1"/>
        <property type="gene ID" value="ENSBIXG00000011497.1"/>
    </dbReference>
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    <dbReference type="OMA" id="MEYEFFW"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="melanoma-associated antigen D1"/>
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    </dbReference>
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        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
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    </dbReference>
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    </feature>
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        <location>
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        </location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="219" mass="24509" checksum="28ECADD96C8CF3C3" modified="2019-09-18" version="1">MPRGRKSRRRRNARAAEENRNSRKIQASETSETPMAASVGSSTPEEDLNCPEEDPSTPEETSTTPEESLSTAQAQKPSVARSNFQGTKKSLLMSILALIFIMGNSAKEALVWKVLGKLGMQPGQQHSIFGDPKKVVTEEFVRRGYLIYKPVPRSSPVEYEFFWGPRAHVESSKLKVMHFVARVRNRCSKDWPCNYDWDSDDDAEVEAILNSGARGYSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DVK9</accession>
    <name>A0A4W2DVK9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SCPEP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in vascular wall and kidney homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DVK9"/>
    <dbReference type="STRING" id="30522.A0A4W2DVK9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010020.1">
        <property type="protein sequence ID" value="ENSBIXP00000028525.1"/>
        <property type="gene ID" value="ENSBIXG00000009764.1"/>
    </dbReference>
    <dbReference type="OMA" id="QEPKEVW"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000075">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021511554" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="232"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="448" mass="50840" checksum="9ECDB36ED24A01F9" modified="2019-09-18" version="1">MELAPRNCPVPLLLLLLGLSTGAVISQPPEDSKEVWGYVTVRKDAHMFWWLYYATSSHKNFSELPLIMWLQGGPGGSSTGFGNFEEIGPLDRNLQPRNTTWLQSASLLFVDNPVGAGFSYVNKTDAYARDLATVVSDMIFLLKSFFDFHKEFERIPFYIFSESYGGKMAAGIALELYKAVQQKTIQCNFAGVALGDAWISPIDSVLSWGPYLYSVSLLDDQGLAEVSNVAEEVLDALSKELYQEATRLWEKAEMVIEQNTDGVNFYNILTKSSSVSRVVSNLEFTQKHLVHLFQRHVRQLQQDPLSQLMNGPIRKKLRIIPEDCIWGGQAPIVFLNMEGDFMKPVISIVDELLEAGVSVTVYNGQLDLIVDTMGQENWMRKLKWKELPKFRQLKWKPLHSDPRSSETSAFVKSYKNLAFYWILRAGHMVPSDQPDMALKMMRLVTQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYK9</accession>
    <name>A0A4W2DYK9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC113893372</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYK9"/>
    <dbReference type="STRING" id="30522.A0A4W2DYK9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006630.1">
        <property type="protein sequence ID" value="ENSBIXP00000029605.1"/>
        <property type="gene ID" value="ENSBIXG00000006880.1"/>
    </dbReference>
    <dbReference type="OMA" id="HHHCPWV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="109"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="271" mass="28931" checksum="E71A5D471E0271C5" modified="2019-09-18" version="1">GRMQHRAHAPSQHLRLLQGPTAAPRVWFVPDALGIVCASATWVLTLSEGVLLLSAWLLPAQRPAYSAVHGSLFHLLASLALASHARTMFTDPGALPVGAPPAPGLAARCPLCGAVQTPGAHHCGVCQRCIRRAHHHCPWVNNCVGEDNRKFFVLFTLYTALGALHVLLLLSAPALRAYARGEWDLQTPVRLRPSLLFLFVVALNGFLFAGLMFTIQIRSILTDPQSQPGEPGQASAWTNLKAVLGPRPSPAWISPFASGGPRAASGPQAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DYZ2</accession>
    <name>A0A4W2DYZ2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DET1 partner of COP1 E3 ubiquitin ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">DET1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027377152.1">
        <property type="nucleotide sequence ID" value="XM_027521351.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027377153.1">
        <property type="nucleotide sequence ID" value="XM_027521352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DYZ2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000016524.1">
        <property type="protein sequence ID" value="ENSBIXP00000032035.1"/>
        <property type="gene ID" value="ENSBIXG00000014504.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005029978.1">
        <property type="protein sequence ID" value="ENSBIXP00005017890.1"/>
        <property type="gene ID" value="ENSBIXG00005021388.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879684"/>
    <dbReference type="CTD" id="55070"/>
    <dbReference type="GeneTree" id="ENSGT00390000005224"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019138">
        <property type="entry name" value="De-etiolated_protein_1_Det1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374:SF3">
        <property type="entry name" value="DET1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374">
        <property type="entry name" value="DET1 HOMOLOG DE-ETIOLATED-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09737">
        <property type="entry name" value="Det1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="550" mass="63825" checksum="884944C728B643C9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0E8</accession>
    <name>A0A4W2E0E8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcipressin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Regulator of calcineurin 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RCAN2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379782.1">
        <property type="nucleotide sequence ID" value="XM_027523981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E0E8"/>
    <dbReference type="SMR" id="A0A4W2E0E8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006605.1">
        <property type="protein sequence ID" value="ENSBIXP00000029613.1"/>
        <property type="gene ID" value="ENSBIXG00000011286.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041567.1">
        <property type="protein sequence ID" value="ENSBIXP00005025712.1"/>
        <property type="gene ID" value="ENSBIXG00005028228.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881165"/>
    <dbReference type="CTD" id="10231"/>
    <dbReference type="GeneTree" id="ENSGT00940000159767"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12709">
        <property type="entry name" value="RRM_RCAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000092">
        <property type="entry name" value="Calcipressin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034919">
        <property type="entry name" value="RCAN2_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF5">
        <property type="entry name" value="CALCIPRESSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="166"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="197" mass="21938" checksum="07E05C383F792D19" modified="2019-09-18" version="1">MPAPSMDCDVSTLVACVVDVEVFTNQEVKEKFEGLFRTYDDCVTFQLFKSFRRVRINFSNPKSAARARIELHETQFRGKKLKLYFAQVQTPETDGDKLHLAPPQPAKQFLISPPASPPVGWQPISDATPVLNYDLLYAVAKLGPGEKYELHAGTESTPSVVVHVCDSDIEEEEDPKTSPKPKIIQTRRPGPPPSVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E1D5</accession>
    <name>A0A4W2E1D5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glial cells missing transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GCM1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027380886.1">
        <property type="nucleotide sequence ID" value="XM_027525085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E1D5"/>
    <dbReference type="SMR" id="A0A4W2E1D5"/>
    <dbReference type="STRING" id="30522.A0A4W2E1D5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005698.1">
        <property type="protein sequence ID" value="ENSBIXP00000029902.1"/>
        <property type="gene ID" value="ENSBIXG00000011654.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034943.1">
        <property type="protein sequence ID" value="ENSBIXP00005041258.1"/>
        <property type="gene ID" value="ENSBIXG00005024212.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881958"/>
    <dbReference type="CTD" id="8521"/>
    <dbReference type="GeneTree" id="ENSGT00390000006777"/>
    <dbReference type="OMA" id="FPLTNWP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060018">
        <property type="term" value="P:astrocyte fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060670">
        <property type="term" value="P:branching involved in labyrinthine layer morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060706">
        <property type="term" value="P:cell differentiation involved in embryonic placenta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060143">
        <property type="term" value="P:positive regulation of syncytium formation by plasma membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060800">
        <property type="term" value="P:regulation of cell differentiation involved in embryonic placenta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000768">
        <property type="term" value="P:syncytium formation by plasma membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3530:FF:000001">
        <property type="entry name" value="Chorion-specific transcription factor GCMb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.670">
        <property type="entry name" value="GCM domain, large subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3530">
        <property type="entry name" value="GCM motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039791">
        <property type="entry name" value="GCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036115">
        <property type="entry name" value="GCM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043020">
        <property type="entry name" value="GCM_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043021">
        <property type="entry name" value="GCM_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003902">
        <property type="entry name" value="Tscrpt_reg_GCM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12414:SF6">
        <property type="entry name" value="CHORION-SPECIFIC TRANSCRIPTION FACTOR GCMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12414">
        <property type="entry name" value="GLIAL CELLS MISSING RELATED/GLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03615">
        <property type="entry name" value="GCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90073">
        <property type="entry name" value="GCM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50807">
        <property type="entry name" value="GCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GCM" evidence="8">
        <location>
            <begin position="13"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="433" mass="48900" checksum="D1186DC54BE88845" modified="2019-09-18" version="1">MEPENADSADKELSWDINDMKLPQNVKKTDWFQEWPDAYEKHIYSSDDRNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCGRDCSVAEGRKVYLRPAICDKARQKQQRKRCPNCDGPLKLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPKPETKLEAEARRAVKKTHSASSSVSSKLKQSPEIKPLPGETQSWENLTWSFQEAVQLPPGYSGHVIGYTPQQKAWNDGSSFTESYGLGGTSELANLTPTLGPPQLYDKWDLSHSRVSSSGDLLQPSISGVFSDYSDLQTWNKNVALGRSPLNDNSCPSYPFPLTSWPYDFSPSQSSSEPFHQPVPVEPPAVKSSYPPIWPNQGGELYEEKVPVDFNSYHPPTTCHSPQEDPLLLTHTSQPYHQYALPGKSNKWDFDEEMGCLGLDHCNNEMLLNLCPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E4D5</accession>
    <name>A0A4W2E4D5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E4D5"/>
    <dbReference type="SMR" id="A0A4W2E4D5"/>
    <dbReference type="STRING" id="30522.A0A4W2E4D5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053221.1">
        <property type="protein sequence ID" value="ENSBIXP00000026524.1"/>
        <property type="gene ID" value="ENSBIXG00000029869.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="97" mass="10501" checksum="E990D0AD9B12A988" modified="2019-09-18" version="1">EMEGREEEETGREKEKQGWPPLQRAGWQLLVGHVHRSLKSGPSSRGLVGLSATGRSAADLEDLTTEESDSVVKAAIAGGEVTPRPHIPAWEERTTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5E4</accession>
    <name>A0A4W2E5E4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cell adhesion molecule 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Immunoglobulin superfamily member 4C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Nectin-like protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CADM4</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371390.1">
        <property type="nucleotide sequence ID" value="XM_027515589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2E5E4"/>
    <dbReference type="SMR" id="A0A4W2E5E4"/>
    <dbReference type="STRING" id="30522.A0A4W2E5E4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021575.1">
        <property type="protein sequence ID" value="ENSBIXP00000034168.1"/>
        <property type="gene ID" value="ENSBIXG00000017096.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005048230.1">
        <property type="protein sequence ID" value="ENSBIXP00005038093.1"/>
        <property type="gene ID" value="ENSBIXG00005020131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876407"/>
    <dbReference type="CTD" id="199731"/>
    <dbReference type="GeneTree" id="ENSGT00940000161223"/>
    <dbReference type="OMA" id="IQNPVRQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044291">
        <property type="term" value="C:cell-cell contact zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043183">
        <property type="term" value="F:vascular endothelial growth factor receptor 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043184">
        <property type="term" value="F:vascular endothelial growth factor receptor 2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030948">
        <property type="term" value="P:negative regulation of vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900747">
        <property type="term" value="P:negative regulation of vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000145">
        <property type="term" value="P:regulation of cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035020">
        <property type="term" value="P:regulation of Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061041">
        <property type="term" value="P:regulation of wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05885">
        <property type="entry name" value="IgI_2_Necl-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000588">
        <property type="entry name" value="Cell adhesion molecule 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000589">
        <property type="entry name" value="cell adhesion molecule 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF3">
        <property type="entry name" value="CELL ADHESION MOLECULE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610981" description="Cell adhesion molecule 4" evidence="19">
        <location>
            <begin position="25"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="224"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="388" mass="42755" checksum="317311B034CDCE9B" modified="2019-09-18" version="1">MGRARRFQWPLLLLWAAAAGPGAGQEVQTENVTVAEGGVAEITCRLHQYDGSIVVIQNPARQTLFFNGTRALKDERFQLEEFSPRRVRIRLSDARLEDEGGYFCQLYTEDTHHQIATLTVLVAPENPIVEVREQAVEGGEVELSCLVPRSRPAAVLRWYRDRKELKGVTSGQENGKVWSVASTVRFRVDRKDDGGIVTCEAQNQALPSGHSKQTQYVLDVQYSPTARIHASQAVVREGDTLVLTCAVTGNPRPNQIRWNRGNESLPERAEAVGETLTLPGLVSADNGTYTCEASNKHGHARALYVLVVYDPGAVVEAQTSVPYAIVGGILALLVFLIICVLVGMVWCSVRQKGSYLTHEASGLDEQGEAREAFLNGSDGHKRKEEFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E8H9</accession>
    <name>A0A4W2E8H9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RAS guanyl releasing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RASGRP3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RASGRP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410789.1">
        <property type="nucleotide sequence ID" value="XM_027554988.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027410790.1">
        <property type="nucleotide sequence ID" value="XM_027554989.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027410791.1">
        <property type="nucleotide sequence ID" value="XM_027554990.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2E8H9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024192.1">
        <property type="protein sequence ID" value="ENSBIXP00000035332.1"/>
        <property type="gene ID" value="ENSBIXG00000018568.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052691.1">
        <property type="protein sequence ID" value="ENSBIXP00005024876.1"/>
        <property type="gene ID" value="ENSBIXG00005027420.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113901085"/>
    <dbReference type="CTD" id="25780"/>
    <dbReference type="GeneTree" id="ENSGT00940000158843"/>
    <dbReference type="OMA" id="QNRVTHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20862">
        <property type="entry name" value="C1_RASGRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000023">
        <property type="entry name" value="RAS guanyl-releasing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000051">
        <property type="entry name" value="Ras guanyl-releasing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000003">
        <property type="entry name" value="Ras guanyl-releasing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000010">
        <property type="entry name" value="ras guanyl-releasing protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF178">
        <property type="entry name" value="RAS GUANYL-RELEASING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 8" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="12">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="10">
        <location>
            <begin position="152"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="458"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="11">
        <location>
            <begin position="494"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="650"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="692" mass="78352" checksum="0D85EC3BF3671D43" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EA22</accession>
    <name>A0A4W2EA22_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acidic leucine-rich nuclear phosphoprotein 32 family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Multifunctional protein that is involved in the regulation of many processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the ANP32 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EA22"/>
    <dbReference type="STRING" id="30522.A0A4W2EA22"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025527.1">
        <property type="protein sequence ID" value="ENSBIXP00000035894.1"/>
        <property type="gene ID" value="ENSBIXG00000019231.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000003">
        <property type="entry name" value="Acidic leucine-rich nuclear phosphoprotein 32 family member A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF2">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="10">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="269" mass="30192" checksum="6686C72843EA1E2D" modified="2019-09-18" version="1">MRKSELGPQSCHVSSSRRPSNCALDQIVVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSDNRICGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDREAPDSDAEVDGVDEEEDDEEGRRCTERRGSVCILHFSSFPGQVWDFHCPGPGIRKLLQLSLMPLVLRSGLMTLTTGTHPHDRNTKVTGDKSDGWGARCHQRRLHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EBC3</accession>
    <name>A0A4W2EBC3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Torsin family 2 member A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family. Torsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EBC3"/>
    <dbReference type="SMR" id="A0A4W2EBC3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027584.1">
        <property type="protein sequence ID" value="ENSBIXP00000036760.1"/>
        <property type="gene ID" value="ENSBIXG00000020261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001014">
        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
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    <sequence length="242" mass="26421" checksum="0D928F30C55DA32E" modified="2019-09-18" version="1">MAAATRSCRPWGSLLGLIWLVSAAAASWDLSSLRCNFGSFCECDFQPDFQGLECDLAQHLAGQHLARSLVVKALKAFLQDPAPTKPLVLSLHGWTGTGKSYVSSLLAHYLFRDGLRSPHVHHFSPVIHFPHPSHLERYKKDLKSWVQGNLTVCSRSLFLFDEMDKLAPGLIEVLRPFLGSSWVVYGTNYRKAIFIFIRWLLALGHHGRASPGRSGALPATPAAPRAALCAQRAGPSGPGAQG</sequence>
</entry>
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                <person name="Tearle R."/>
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    <sequence length="1452" mass="159512" checksum="13A9CBE3E123938A" modified="2019-09-18" version="1"> </sequence>
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                <person name="Bickhart D.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="157" mass="17301" checksum="CF6B362D7FFB63B7" modified="2019-09-18" version="1">MGSRPTPGLQRTTSAEYRLPSTRPPASVPSTAPGGPVVGRGPTGGPQAPKAKWSALGADGVQRDQLWRELLEAERRSQQRWAQNWSFLKDYDPMGNKKEPVKLPDHVPRFSDTVPNSTNRAVGSRVDTPLGKTLIGLDFFFVEGARKKKLEEELQPI</sequence>
</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Phillippy A.M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 2"/>
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    </dbReference>
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        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 1 variant"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="12">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
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            <dbReference type="Rhea" id="RHEA:16958"/>
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    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
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    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
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        <cofactor evidence="16">
            <name>a divalent metal cation</name>
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        <text evidence="16">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
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        <subcellularLocation>
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        <property type="entry name" value="PDEase_CS"/>
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        <property type="entry name" value="PDEASE_I_2"/>
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    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
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            <end position="99"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <end position="240"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="region of interest" description="Disordered" evidence="17">
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        <location>
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <begin position="46"/>
            <end position="76"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="590"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="727" mass="81643" checksum="55075ECECA620A94" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EM13</accession>
    <name>A0A4W2EM13_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dTDP-D-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TGDS</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027413606.1">
        <property type="nucleotide sequence ID" value="XM_027557805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EM13"/>
    <dbReference type="SMR" id="A0A4W2EM13"/>
    <dbReference type="STRING" id="30522.A0A4W2EM13"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029646.1">
        <property type="protein sequence ID" value="ENSBIXP00000037680.1"/>
        <property type="gene ID" value="ENSBIXG00000021277.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053983.1">
        <property type="protein sequence ID" value="ENSBIXP00005045311.1"/>
        <property type="gene ID" value="ENSBIXG00005001934.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113902476"/>
    <dbReference type="CTD" id="23483"/>
    <dbReference type="GeneTree" id="ENSGT00940000159196"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="8">
        <location>
            <begin position="21"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="355" mass="40666" checksum="871C965B58BEF68C" modified="2019-09-18" version="1">MSVAGRAESLGPPDSFAKRVLVTGGAGFIASHMIVSLVEDYPNYMIINLDKLDYCASLKNLETISNKQNYKFIQGDICDSHFVKLLFETEKIDIVLHFAAQTHVDLSFVRAFEFTYVNVYGTHVLVSAAHEARVEKFIYVSTDEVYGGSLDKEFDESSPKQPTNPYASSKAAAECFVQSYWEQYKFPVVITRSSNVYGPHQYPEKVIPKFISLLQHNRKCCIHGTGLQTRNFLYATDVVEAFLTVLKKGKPGEIYNIGTNFEMSVLQLAKELIQLIKETNSESEMENWVDYVDDRPTNDMRYPMKSEKIHGLGWRPKVPWKEGIKKTIEWYRENFHNWKNAEKALEPFPVQPPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EP59</accession>
    <name>A0A4W2EP59_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EP59"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031921.1">
        <property type="protein sequence ID" value="ENSBIXP00000038661.1"/>
        <property type="gene ID" value="ENSBIXG00000004156.1"/>
    </dbReference>
    <dbReference type="OMA" id="FIMTTHA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="66"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="375" mass="43194" checksum="C42D92EC3380FBB1" modified="2019-09-18" version="1">MKRQNDRILKEKLPRSVGAQYATGDQWRNNSRKNEGMEPKQKPYPAVDVTGDRSKVRCCKEQYCIGTWNVSSMNQGKLQVIKQEMARVNVDILGISELKWTGMGEFNSDDHYIYYCGQESLRRNGVAIMVNKRVRNAVLGCNLKNDRMISVRFQGKPFNIAVIQAYAPTSNAEEAEVERFYEDLQDLLELTPKKDVLFIIGDWNAKVGSQETPGVTGKFGLGIRNEAGQRLIEFCQENALVITNTLFQQHKRRLYTWTSPDGQHQNQIDYILCSQRWRSSIQSAKTRPGADCGSDHELLIAKFRLKLKKVGKTTRLFRYDLNQIPYDYTVEVRNRFKGLDLIDRVPDELWNEVLDIVQETGIKTIPMEKKCKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EPA6</accession>
    <name>A0A4W2EPA6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EPA6"/>
    <dbReference type="STRING" id="30522.A0A4W2EPA6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032140.1">
        <property type="protein sequence ID" value="ENSBIXP00000038722.1"/>
        <property type="gene ID" value="ENSBIXG00000022617.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120206">
        <property type="term" value="C:photoreceptor distal connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120200">
        <property type="term" value="C:rod photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SPATA7"/>
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        <source>
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        <source>
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</entry>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    </evidence>
    <sequence length="83" mass="9311" checksum="6CEFEEEA2A918648" modified="2019-09-18" version="1">MNLSDQEAKPSTEDLTGKKEGEYIKLKVTGQESSEIHFQVKMMTHFKKLKESHCQRQGAPVNSPRRCDLDSCAHDSLSSVSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ERZ3</accession>
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            <fullName evidence="6">Suppressor of tumorigenicity 7 protein-like</fullName>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUT9</accession>
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        <submittedName>
            <fullName evidence="3">Otospiralin</fullName>
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    <reference evidence="3 4" key="1">
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            <title>Haplotype-resolved cattle genomes.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="184" mass="19002" checksum="683243682249B3EE" modified="2019-09-18" version="1">MKTLRTCLFTGAVFLGGAHLGVAGGGGDLGRRGSGGGRRRQGSGAGASHPSGFLRLQVAGVALGKMPARLPPGLVLCLLLAPLAGGGRLSSCAVPVGGGVPPRGLGVTALPSLAGAKPVQEEGDPHAELPAMPYWPFSTSDFWNYVQHFQALGAYPQLEDMARTFFAHFPLGTTLGFHVPYREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVV9</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="11">Sorting nexin-27</fullName>
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    </protein>
    <gene>
        <name evidence="16" type="primary">SNX27</name>
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    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027389345.1">
        <property type="nucleotide sequence ID" value="XM_027533544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EVV9"/>
    <dbReference type="SMR" id="A0A4W2EVV9"/>
    <dbReference type="STRING" id="30522.A0A4W2EVV9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000037248.1">
        <property type="protein sequence ID" value="ENSBIXP00000040919.1"/>
        <property type="gene ID" value="ENSBIXG00000005121.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005053813.1">
        <property type="protein sequence ID" value="ENSBIXP00005026656.1"/>
        <property type="gene ID" value="ENSBIXG00005005088.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886889"/>
    <dbReference type="CTD" id="81609"/>
    <dbReference type="GeneTree" id="ENSGT00950000183212"/>
    <dbReference type="OMA" id="PNEFPHN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098842">
        <property type="term" value="C:postsynaptic early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098837">
        <property type="term" value="C:postsynaptic recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090128">
        <property type="term" value="P:regulation of synapse maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13338">
        <property type="entry name" value="FERM-like_C_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01777">
        <property type="entry name" value="FERM_F1_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23070">
        <property type="entry name" value="PDZ_SNX27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06886">
        <property type="entry name" value="PX_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.60:FF:000002">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000061">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000075">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000003">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037827">
        <property type="entry name" value="SNX27_FERM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037833">
        <property type="entry name" value="SNX27_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037835">
        <property type="entry name" value="SNX27_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF19">
        <property type="entry name" value="SORTING NEXIN-27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="43"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="161"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="15">
        <location>
            <begin position="273"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="541" mass="61127" checksum="4A7DFBEE886FDE0C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EW97</accession>
    <name>A0A4W2EW97_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EW97"/>
    <dbReference type="STRING" id="30522.A0A4W2EW97"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038023.1">
        <property type="protein sequence ID" value="ENSBIXP00000041313.1"/>
        <property type="gene ID" value="ENSBIXG00000025533.1"/>
    </dbReference>
    <dbReference type="OMA" id="NIIKSGW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="217" mass="22454" checksum="43752AA8AB23817D" modified="2019-09-18" version="1">MSQPGIPASGGSSTGLQAQNGAASASGSPYTNGPVQNALMSSQVSVSQGYNSQLPGSYPHLIPAKTLNPVSGQSNSGGSQTVSPLSNYQGPGQALYGPPVASHPVTPSLRSGPSPQMPLPTSQNPAATPMPSGSFLPGASVSSPSNWQYNYLSQTNHCPRASSQPTMTGNTNLMSPQYVSSGDSSLQNNIIKSGWKCNLCYRVNDGMFFKLILLYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXL5</accession>
    <name>A0A4W2EXL5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ferroxidase</fullName>
            <ecNumber evidence="2">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113893980</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EXL5"/>
    <dbReference type="STRING" id="30522.A0A4W2EXL5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039172.1">
        <property type="protein sequence ID" value="ENSBIXP00000041840.1"/>
        <property type="gene ID" value="ENSBIXG00000026026.1"/>
    </dbReference>
    <dbReference type="OMA" id="ENIVHRV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000015">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000033">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024715">
        <property type="entry name" value="Factor_5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF314">
        <property type="entry name" value="CERULOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000354">
        <property type="entry name" value="Factors_V_VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021501052" description="ferroxidase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="96"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="11">
        <location>
            <begin position="230"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="437"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="793"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="927"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="279"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="524"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="627"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000354-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1043" mass="118636" checksum="460AF9172577C888" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F389</accession>
    <name>A0A4W2F389_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sentrin-specific protease 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">SUMO-1-specific protease 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Sentrin/SUMO-specific protease SENP7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SENP7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Protease that acts as a positive regulator of the cGAS-STING pathway by catalyzing desumoylation of CGAS. Desumoylation of CGAS promotes DNA-binding activity of CGAS, subsequent oligomerization and activation. Deconjugates SUMO2 and SUMO3 from targeted proteins, but not SUMO1. Catalyzes the deconjugation of poly-SUMO2 and poly-SUMO3 chains. Has very low efficiency in processing full-length SUMO proteins to their mature forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C48 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027561714.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027417531.1">
        <property type="nucleotide sequence ID" value="XM_027561730.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2F389"/>
    <dbReference type="STRING" id="30522.A0A4W2F389"/>
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        <property type="protein sequence ID" value="ENSBIXP00000043891.1"/>
        <property type="gene ID" value="ENSBIXG00000028070.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023720.1">
        <property type="protein sequence ID" value="ENSBIXP00005036310.1"/>
        <property type="gene ID" value="ENSBIXG00005018029.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904478"/>
    <dbReference type="CTD" id="57337"/>
    <dbReference type="GeneTree" id="ENSGT00940000157308"/>
    <dbReference type="OMA" id="EFIFLEI"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099524">
        <property type="term" value="C:postsynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099523">
        <property type="term" value="C:presynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.20:FF:000001">
        <property type="entry name" value="sentrin-specific protease 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000001">
        <property type="entry name" value="sentrin-specific protease 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.20:FF:000004">
        <property type="entry name" value="sentrin-specific protease 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000002">
        <property type="entry name" value="SUMO specific peptidase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF2">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="15">
        <location>
            <begin position="757"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="193"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="440"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="883"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="271"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="887"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="901"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1047" mass="118889" checksum="BD79A921437E86CF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3H2</accession>
    <name>A0A4W2F3H2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Queuine tRNA-ribosyltransferase catalytic subunit 1</fullName>
            <ecNumber evidence="8">2.4.2.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">QTRT1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>guanosine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:16633"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:18571"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="EC" id="2.4.2.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion outer membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="8">Weakly associates with mitochondria, possibly via QTRT2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.64" evidence="8"/>
    <dbReference type="RefSeq" id="XP_027403528.1">
        <property type="nucleotide sequence ID" value="XM_027547727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3H2"/>
    <dbReference type="STRING" id="30522.A0A4W2F3H2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043074.1">
        <property type="protein sequence ID" value="ENSBIXP00000043974.1"/>
        <property type="gene ID" value="ENSBIXG00000028179.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052744.1">
        <property type="protein sequence ID" value="ENSBIXP00005043746.1"/>
        <property type="gene ID" value="ENSBIXG00005027486.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895907"/>
    <dbReference type="CTD" id="81890"/>
    <dbReference type="GeneTree" id="ENSGT00530000063679"/>
    <dbReference type="OMA" id="IDLFDCV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032473">
        <property type="term" value="C:cytoplasmic side of mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120507">
        <property type="term" value="C:tRNA-guanine transglycosylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.105:FF:000001">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="9">
        <location>
            <begin position="26"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="8">
        <location>
            <begin position="260"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="8">
        <location>
            <begin position="284"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="403" mass="44245" checksum="9C628B1C777321CB" modified="2019-09-18" version="1">MAAAASQTSLESAPRIMRLVAECSRSRARAGELRLPHGPVATPVFMPVGTQATMKGVTAEQLDALGCRICLGNTYHLGLRPGPELIQKAQGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGDETLLSPERSVEIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHRRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGFAIGGLSGGESKGEFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQLKKKQYEKDFRPIDPECDCPTCQKHSRAFLHTLLHSDNTAALHHLTIHNIAYQLRLMSAVRASIVEKRFPAFVRDFMSTMYGDPTLCPTWATEALASVGITLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F5L2</accession>
    <name>A0A4W2F5L2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L22-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC113878981</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027375998.1">
        <property type="nucleotide sequence ID" value="XM_027520197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F5L2"/>
    <dbReference type="SMR" id="A0A4W2F5L2"/>
    <dbReference type="STRING" id="30522.A0A4W2F5L2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044933.1">
        <property type="protein sequence ID" value="ENSBIXP00000033591.1"/>
        <property type="gene ID" value="ENSBIXG00000016424.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000264.1">
        <property type="protein sequence ID" value="ENSBIXP00005000514.1"/>
        <property type="gene ID" value="ENSBIXG00005030123.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113878981"/>
    <dbReference type="GeneTree" id="ENSGT00940000154509"/>
    <dbReference type="OMA" id="FTHPIED"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000001">
        <property type="entry name" value="60S ribosomal protein L22 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN EL22-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005000514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="122" mass="14533" checksum="67D0A941A07E68FC" modified="2019-09-18" version="1">MVQKKDKKPKKSTWKFNLEFTHPVEDGVFDSGNVEQFMLEKVKVNGKTGNLGNVVHIEHFKNKIIVVSEKQFSKRYLKYFTKKYLKKNSLHDWLPVVASDKETYELRYFQIRQDEDESESKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F990</accession>
    <name>A0A4W2F990_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-phase kinase-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cyclin-A/CDK2-associated protein p45</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">F-box protein Skp2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SKP2</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Following phosphorylation in response to DNA damage, mediates 'Lys-63'-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027376271.1">
        <property type="nucleotide sequence ID" value="XM_027520470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F990"/>
    <dbReference type="STRING" id="30522.A0A4W2F990"/>
    <dbReference type="Ensembl" id="ENSBIXT00000019466.1">
        <property type="protein sequence ID" value="ENSBIXP00000010580.1"/>
        <property type="gene ID" value="ENSBIXG00000016033.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005013460.1">
        <property type="protein sequence ID" value="ENSBIXP00005001822.1"/>
        <property type="gene ID" value="ENSBIXG00005008301.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879182"/>
    <dbReference type="CTD" id="6502"/>
    <dbReference type="GeneTree" id="ENSGT00390000007918"/>
    <dbReference type="OMA" id="CDFTADH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905168">
        <property type="term" value="P:positive regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22114">
        <property type="entry name" value="F-box_FBXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000105">
        <property type="entry name" value="S-phase kinase-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976:SF1">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="16">
        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="436" mass="49118" checksum="D9635746B46F19DB" modified="2019-09-18" version="1">MHAFKTPEAVDAMHRKHLQEIPDQSSNVTTSFTWGWDSSKTSELLSGMGVSALEKEEVDSENIPQELLSNLGHPQSPPRKRLKSKGNDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSSVCKRWYHLAFDESLWQTVDLAGRNLYPDVVGRLLSRGVVAFRCPRSFMDQPLVEHFSPFRLQHLDLSNSVIDASTLHGLLSHCSKLQNLSLEGLRLSDPVVDNLAQNTNLLRLNLSGCSGFSESALKTLLSSCSRLDELNLSWCYDFTEKHVQMAVAHVSKTITQLNLSGYRKNLQRSDVSTLVGRCPNLVHLDLSDSVMLKNDCFPEFYQLNYLQHLSLSRCYDIIPETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPHLQINCSHFTTIARPTIGNKKNQEIWGIKCRLSLEKPSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F9B8</accession>
    <name>A0A4W2F9B8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EIF2S1</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408018.1">
        <property type="nucleotide sequence ID" value="XM_027552217.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027408019.1">
        <property type="nucleotide sequence ID" value="XM_027552218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2F9B8"/>
    <dbReference type="SMR" id="A0A4W2F9B8"/>
    <dbReference type="STRING" id="30522.A0A4W2F9B8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034835.1">
        <property type="protein sequence ID" value="ENSBIXP00000039907.1"/>
        <property type="gene ID" value="ENSBIXG00000023923.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014639.1">
        <property type="protein sequence ID" value="ENSBIXP00005001227.1"/>
        <property type="gene ID" value="ENSBIXG00005029373.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899005"/>
    <dbReference type="CTD" id="1965"/>
    <dbReference type="GeneTree" id="ENSGT00390000007015"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000795">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FIE6</accession>
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            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FJB2</accession>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384871.1">
        <property type="nucleotide sequence ID" value="XM_027529070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FJB2"/>
    <dbReference type="STRING" id="30522.A0A4W2FJB2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003850.1">
        <property type="protein sequence ID" value="ENSBIXP00000006396.1"/>
        <property type="gene ID" value="ENSBIXG00000012508.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005006281.1">
        <property type="protein sequence ID" value="ENSBIXP00005005483.1"/>
        <property type="gene ID" value="ENSBIXG00005011167.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884464"/>
    <dbReference type="CTD" id="282991"/>
    <dbReference type="GeneTree" id="ENSGT00390000005889"/>
    <dbReference type="OMA" id="CSDMFEK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005005483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="146" mass="16389" checksum="1D19AF1695DAD03B" modified="2019-09-18" version="1">MAAAAAAAAEGVPAIHREEPVRDDVAVETAEEAKEPAEADINELCRDMFSKMATYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAGLQPYLDQINIIEEQVAALEQAAYKLDAYSKKLEAKYKKLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FQP8</accession>
    <name>A0A4W2FQP8_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S ribosomal protein L31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPL31</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027410512.1">
        <property type="nucleotide sequence ID" value="XM_027554711.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027410513.1">
        <property type="nucleotide sequence ID" value="XM_027554712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FQP8"/>
    <dbReference type="SMR" id="A0A4W2FQP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030090.1">
        <property type="protein sequence ID" value="ENSBIXP00000037860.1"/>
        <property type="gene ID" value="ENSBIXG00000021534.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001667.1">
        <property type="protein sequence ID" value="ENSBIXP00005007779.1"/>
        <property type="gene ID" value="ENSBIXG00005013066.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900969"/>
    <dbReference type="CTD" id="6160"/>
    <dbReference type="GeneTree" id="ENSGT00950000183030"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="125" mass="14463" checksum="BA9DBE79B9E1C071" modified="2019-09-18" version="1">MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FYF5</accession>
    <name>A0A4W2FYF5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2FYF5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001070.1">
        <property type="protein sequence ID" value="ENSBIXP00000007808.1"/>
        <property type="gene ID" value="ENSBIXG00000001433.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005019434.1">
        <property type="protein sequence ID" value="ENSBIXP00005010849.1"/>
        <property type="gene ID" value="ENSBIXG00005015408.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286929"/>
    <dbReference type="OMA" id="LAGCENW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="182"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="366" mass="42537" checksum="9902C503E7289F07" modified="2019-09-18" version="1">MEKKCKKAKWLSGEALQIAVKRREVKSKGEKERYKHLNAEFQSIARRDKKAFFSDQCKEIVENNRMGKTRDLFKKIRDTKGTFHAKMGSIKDRNGTDLTEAEDIKKRWQKYTEELYKKDLHDPDNHDGVITDLEPDILECEVKWALESSTTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCILRNPYAGQKATVRTGHGTTDWFQIGKGVRQGCILSTCLFNFYTEYIMRNAGLEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G1A9</accession>
    <name>A0A4W2G1A9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAK4-inhibitor INKA2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">PAK4-inhibitor inka2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">INKA2</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Inhibitor of the serine/threonine-protein kinase PAK4. Acts by binding PAK4 in a substrate-like manner, inhibiting the protein kinase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PAK4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INKA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027391974.1">
        <property type="nucleotide sequence ID" value="XM_027536173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G1A9"/>
    <dbReference type="STRING" id="30522.A0A4W2G1A9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049393.1">
        <property type="protein sequence ID" value="ENSBIXP00000039753.1"/>
        <property type="gene ID" value="ENSBIXG00000023702.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021355.1">
        <property type="protein sequence ID" value="ENSBIXP00005012145.1"/>
        <property type="gene ID" value="ENSBIXG00005016422.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889466"/>
    <dbReference type="CTD" id="55924"/>
    <dbReference type="GeneTree" id="ENSGT00530000063849"/>
    <dbReference type="OMA" id="QPEPPRW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000319">
        <property type="entry name" value="Inka box actin regulator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029267">
        <property type="entry name" value="FAM212"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039201">
        <property type="entry name" value="Inka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28615">
        <property type="entry name" value="PAK4-INHIBITOR INKA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28615:SF2">
        <property type="entry name" value="PAK4-INHIBITOR INKA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15342">
        <property type="entry name" value="FAM212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM212" evidence="9">
        <location>
            <begin position="114"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="223"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="294" mass="31839" checksum="19DEF7B0B3B0F658" modified="2019-09-18" version="1">MDCYLRRLKQELMSMKEVGDGLQDQMNCMMGALQELKLLQVQTALEQLEISGGGPAPGSPESPRTQLEPPQWEGSRGPVRPGACSPSNQASLGSTSSGKFPSHRSVCGRELATPPRTPLPEPQPSAQQGPELAEPDDWTSTLMSRGRNRQPLVLGDNVFADLVGNWLDLPELEKGGEKGETGEAGEPKGGRGQPRELGRRFALTANIFRKFLRSVRPDRDRLLKEKPGWVTPTASEPRAGRSQKVKKRSHSKGSGHCPFPGASEPRRGENASTGCPKALESSPSGFDINTAVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G549</accession>
    <name>A0A4W2G549_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HERC3</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027399888.1">
        <property type="nucleotide sequence ID" value="XM_027544087.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399889.1">
        <property type="nucleotide sequence ID" value="XM_027544088.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399890.1">
        <property type="nucleotide sequence ID" value="XM_027544089.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399891.1">
        <property type="nucleotide sequence ID" value="XM_027544090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G549"/>
    <dbReference type="SMR" id="A0A4W2G549"/>
    <dbReference type="STRING" id="30522.A0A4W2G549"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051468.1">
        <property type="protein sequence ID" value="ENSBIXP00000042448.1"/>
        <property type="gene ID" value="ENSBIXG00000007732.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046587.1">
        <property type="protein sequence ID" value="ENSBIXP00005013271.1"/>
        <property type="gene ID" value="ENSBIXG00005017431.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894135"/>
    <dbReference type="CTD" id="8916"/>
    <dbReference type="GeneTree" id="ENSGT00940000158189"/>
    <dbReference type="OMA" id="FVLCSEY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000012">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000018">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000010">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF18">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="53"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="103"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="156"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="209"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="261"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="314"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="11">
        <location>
            <begin position="723"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005013271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1050" mass="117076" checksum="E9DD0A78E137A2E7" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIN3</accession>
    <name>A0A4W2GIN3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">KLC3</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa. May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Oligomer composed of two heavy chains and two light chains. Associates with microtubulin in an ATP-dependent manner. Interacts with KIF5C. Interacts with ODF1. Interacts with LRGUK. Interacts with VDAC2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Cytoplasm</location>
            <location evidence="2 18">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GIN3"/>
    <dbReference type="STRING" id="30522.A0A4W2GIN3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022069.1">
        <property type="protein sequence ID" value="ENSBIXP00000012260.1"/>
        <property type="gene ID" value="ENSBIXG00000002893.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049382.1">
        <property type="protein sequence ID" value="ENSBIXP00005018684.1"/>
        <property type="gene ID" value="ENSBIXG00005022032.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162356"/>
    <dbReference type="OMA" id="HTSGHCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008088">
        <property type="term" value="P:axo-dendritic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120317">
        <property type="term" value="P:sperm mitochondrial sheath assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF1">
        <property type="entry name" value="KINESIN LIGHT CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 18" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="9 17" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="364"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="25"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="230"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="482"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="161"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="562"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="578" mass="63112" checksum="C45B402019131F3F" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GL32</accession>
    <name>A0A4W2GL32_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N6-Methyl-AMP deaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Adenosine deaminase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MAPDA</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-methyl-AMP + H2O + H(+) = IMP + methylamine</text>
            <dbReference type="Rhea" id="RHEA:16001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16002"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GL32"/>
    <dbReference type="SMR" id="A0A4W2GL32"/>
    <dbReference type="STRING" id="30522.A0A4W2GL32"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017217.1">
        <property type="protein sequence ID" value="ENSBIXP00000032311.1"/>
        <property type="gene ID" value="ENSBIXG00000014848.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031204.1">
        <property type="protein sequence ID" value="ENSBIXP00005018709.1"/>
        <property type="gene ID" value="ENSBIXG00005003738.1"/>
    </dbReference>
    <dbReference type="CTD" id="161823"/>
    <dbReference type="GeneTree" id="ENSGT00950000183113"/>
    <dbReference type="OMA" id="RPQFKPY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097305">
        <property type="term" value="P:response to alcohol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00443">
        <property type="entry name" value="ADA_AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000033">
        <property type="entry name" value="Adenosine deaminase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF42">
        <property type="entry name" value="ADENOSINE DEAMINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="351" mass="39646" checksum="258E442F7867F481" modified="2019-09-18" version="1">MMEAEEQPWKTTFYSKLPKVELHAHLNGSISSNTIRKLIAKKPDLKIHDQMTMIDKGEKRTLEECLQMFQIIHLLTTTPEDVLMVTKDVIKEFADDGVKYLELRSTPRGEDATGMTKKTYVESILEGIKQSKEENVDIDVRYLISIDRRGGSSAAKEAVKLAEEFFLSAEDTVLGLDLSGDPSAGQAKDFLEPLLEAKKSGLKLALHLSEIPNQKTETQVLLNLFPDRIGHGTFLSSSEEGSPDLVDFVRQHQIPLELCLTSNVKSQTVPAYDQHHFGFWYSVAHPAVICTDDKGVFATRLSQEYQLVAETFHLTQSQVWDLSYESISYIFASDSTKADLRKKWSHLKPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GN39</accession>
    <name>A0A4W2GN39_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-crystallin A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Beta-A2 crystallin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CRYBA2</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027369393.1">
        <property type="nucleotide sequence ID" value="XM_027513592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GN39"/>
    <dbReference type="SMR" id="A0A4W2GN39"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046490.1">
        <property type="protein sequence ID" value="ENSBIXP00000035779.1"/>
        <property type="gene ID" value="ENSBIXG00000019108.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049731.1">
        <property type="protein sequence ID" value="ENSBIXP00005019361.1"/>
        <property type="gene ID" value="ENSBIXG00005022597.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875275"/>
    <dbReference type="CTD" id="1412"/>
    <dbReference type="GeneTree" id="ENSGT00940000160306"/>
    <dbReference type="OMA" id="SDCANIA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000012">
        <property type="entry name" value="Beta-crystallin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000004">
        <property type="entry name" value="Crystallin beta A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF7">
        <property type="entry name" value="BETA-CRYSTALLIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="53"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="148"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="197" mass="22231" checksum="B673513089AC7DE9" modified="2019-09-18" version="1">MSSAPAQGPAPASLTLWDEEDFQGRRCRLLSDCANIGERGGLRRVRSVKVENGAWVAFEYPDFQGQQFILEKGDYPRWSAWSGSAGHHSDQLLSFRPVLCANHSDSRVTLFEGENFQGCKFELNDDYPSLPSMGWASKDVGSLKVSSGAWVAYQYPGYRGYQYVLERDHHSGEFRNYSEFGTQAHTGQLQSIRRVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GT77</accession>
    <name>A0A4W2GT77_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Serine/threonine-protein kinase Nek2</fullName>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Never in mitosis A-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">NEK2</name>
    </gene>
    <organism evidence="35 37">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36 37" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Centromere</location>
            <location evidence="4">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027421467.1">
        <property type="nucleotide sequence ID" value="XM_027565666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GT77"/>
    <dbReference type="SMR" id="A0A4W2GT77"/>
    <dbReference type="STRING" id="30522.A0A4W2GT77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001150.1">
        <property type="protein sequence ID" value="ENSBIXP00000031418.1"/>
        <property type="gene ID" value="ENSBIXG00000006087.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005034565.1">
        <property type="protein sequence ID" value="ENSBIXP00005020973.1"/>
        <property type="gene ID" value="ENSBIXG00005004109.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906910"/>
    <dbReference type="CTD" id="4751"/>
    <dbReference type="GeneTree" id="ENSGT00940000156989"/>
    <dbReference type="OMA" id="LALHRCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001824">
        <property type="term" value="P:blastocyst development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051299">
        <property type="term" value="P:centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903126">
        <property type="term" value="P:negative regulation of centriole-centriole cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032206">
        <property type="term" value="P:positive regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051988">
        <property type="term" value="P:regulation of attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046602">
        <property type="term" value="P:regulation of mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000151">
        <property type="entry name" value="G2-specific protein kinase nimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000356">
        <property type="entry name" value="Serine/threonine-protein kinase Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000310">
        <property type="entry name" value="serine/threonine-protein kinase Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
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    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
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    <keyword evidence="21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="27" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="12" id="KW-0132">Cell division</keyword>
    <keyword evidence="28" id="KW-0137">Centromere</keyword>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="19" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="23 33" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="26" id="KW-0469">Meiosis</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="17" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="8"/>
            <end position="271"/>
        </location>
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        <location>
            <begin position="303"/>
            <end position="333"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00067730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00082682"/>
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    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005020973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="51476" checksum="26B653DF124C99F3" modified="2019-09-18" version="1">MPTRVEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTENEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMECCEGGDLASVIAKGTKERQYLDEEFVLRVMAQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNHMSYNEKSDIWSLGCLLYELCALMPPFTAFNQKELAGKIREGKFRRIPYRYSDELNDIITRMLNLKDYHRPSVEEILENPLIANLVAEEQRRNPERRGRRIGEPEKLQDSSPVLSELKLKEIQLQEREAALKAREESLEQRERELCVRERLAENKLARAEGLLKNYSLLKEQRFLSLAGGPELFDLPSSVVKKKVHFSGESKENVIRGENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKTYQLKSRQILGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GX25</accession>
    <name>A0A4W2GX25_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neurogenin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NEUROG2</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_027543817.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A4W2GX25"/>
    <dbReference type="STRING" id="30522.A0A4W2GX25"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042416.1">
        <property type="protein sequence ID" value="ENSBIXP00000025652.1"/>
        <property type="gene ID" value="ENSBIXG00000027726.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038846.1">
        <property type="protein sequence ID" value="ENSBIXP00005023829.1"/>
        <property type="gene ID" value="ENSBIXG00005026506.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894017"/>
    <dbReference type="CTD" id="63973"/>
    <dbReference type="GeneTree" id="ENSGT00940000162895"/>
    <dbReference type="OMA" id="GLVHECK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19717">
        <property type="entry name" value="bHLH_TS_NGN2_ATOH4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
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        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032655">
        <property type="entry name" value="Ngn-2_bHLH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF171">
        <property type="entry name" value="NEUROGENIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="112"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="195"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="270" mass="28541" checksum="02F4E6A85CF82D24" modified="2019-09-18" version="1">MFVKSETLELKEEEDVLVLLGSASPASAALTPVSSSADEEEEEELGAAGGARRQLGAEAGPAALGGSPGGAEGCRPARLLGVVHECKRRPSRARAVSRGAKTAETVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWALTETLRLADHCGGGSLPGALFSEAVILSPGGTSAALSNSGDSPSPASTWSCTNSPAESSSASSNSTSPYSCTLSPASPEGSDMDYWQPPPPDKHRYAPHLPIVRDCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H0M9</accession>
    <name>A0A4W2H0M9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NK2 homeobox 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NKX2-8</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NK-2 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027377498.1">
        <property type="nucleotide sequence ID" value="XM_027521697.1"/>
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    <dbReference type="STRING" id="30522.A0A4W2H0M9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018146.1">
        <property type="protein sequence ID" value="ENSBIXP00000009719.1"/>
        <property type="gene ID" value="ENSBIXG00000002197.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038216.1">
        <property type="protein sequence ID" value="ENSBIXP00005023392.1"/>
        <property type="gene ID" value="ENSBIXG00005007637.1"/>
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    <dbReference type="GeneID" id="113879874"/>
    <dbReference type="CTD" id="26257"/>
    <dbReference type="GeneTree" id="ENSGT00940000161610"/>
    <dbReference type="OMA" id="AWRDFWS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050673">
        <property type="term" value="P:epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030324">
        <property type="term" value="P:lung development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050680">
        <property type="term" value="P:negative regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NK2 homeobox 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF27">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-2.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="82"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="84"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="237" mass="25887" checksum="F52073ABACC48B41" modified="2019-09-18" version="1">MATSGRLSFTVRRLLDLPEQDAQHLRKREPELSAPEPGPCATWLESERGHYPSSDESSPEASPRDSSQRPSARPASPGSDAEKRKKRRVLFSKAQTLELERRFRQQRYLSAPEREQLARLLRLTPTQVKIWFQNHRYKLKRARAPGVAEAPDLAAAAEVRAAPGLLRRVVVPVLVRDGQPCGGSAEGGTAAARDKSAAPPAAACPVPGYAAFGPGSAFGLFPAYQHLAPPALVSWNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H5F6</accession>
    <name>A0A4W2H5F6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trans-2,3-enoyl-CoA reductase-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Steroid 5-alpha-reductase 2-like 2 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TECRL</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027400415.1">
        <property type="nucleotide sequence ID" value="XM_027544614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H5F6"/>
    <dbReference type="STRING" id="30522.A0A4W2H5F6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050187.1">
        <property type="protein sequence ID" value="ENSBIXP00000021861.1"/>
        <property type="gene ID" value="ENSBIXG00000024864.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043880.1">
        <property type="protein sequence ID" value="ENSBIXP00005027151.1"/>
        <property type="gene ID" value="ENSBIXG00005001707.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894329"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
    <dbReference type="OMA" id="RQVSWTT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17125">
        <property type="entry name" value="Ubl_TECRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000131">
        <property type="entry name" value="trans-2,3-enoyl-CoA reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049127">
        <property type="entry name" value="TECR-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047822">
        <property type="entry name" value="TECRL_Ubl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF27">
        <property type="entry name" value="TRANS-2,3-ENOYL-COA REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21696">
        <property type="entry name" value="TECR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="13">
        <location>
            <begin position="75"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="228"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="378" mass="43815" checksum="F83D9100FCB603FC" modified="2019-09-18" version="1">MCLLLTRQLLLVVCRMFKRHKSLASERRRELISRGATRSILKDDMKNFHFLSQFILSAGPLKSTSAVKHSKTIHFEIEILDAQTKKQICIVDKVTQKSTIHDVKQKFHKACPQWYPSRVGLQLERGGPFLKDNLTIQSVAASSIVTLYFTDLGQQVSWTTVFLAEYTGPLLIYLLFYLRIPYIYNMKESSRRLCHPVVHLACFCHCIHYIRYLLETLFVHKVSSGHTSLKNLLKSCAFYWGFTSWIAYYINHPRYTPPSFGYRQVAISAINFLICEAGNHFINVVLSHPSHTGNNACFPSPNYNPFTWMFFLVSCPNYTYEIGSWISFTIMTQTLPVGIFTLLMSIQMSLWAKKKHKIYLKKFSSYMHRKSAMIPFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H6S5</accession>
    <name>A0A4W2H6S5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myotubularin related protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MTMR14</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027377996.1">
        <property type="nucleotide sequence ID" value="XM_027522195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H6S5"/>
    <dbReference type="STRING" id="30522.A0A4W2H6S5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013392.1">
        <property type="protein sequence ID" value="ENSBIXP00000027421.1"/>
        <property type="gene ID" value="ENSBIXG00000008249.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041799.1">
        <property type="protein sequence ID" value="ENSBIXP00005025861.1"/>
        <property type="gene ID" value="ENSBIXG00005028344.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880292"/>
    <dbReference type="CTD" id="64419"/>
    <dbReference type="GeneTree" id="ENSGT00390000018852"/>
    <dbReference type="OMA" id="CALKTHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="PH-GRAM_MTMR14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039802">
        <property type="entry name" value="MTMR14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039803">
        <property type="entry name" value="MTMR14_PH-GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13524">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13524:SF2">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="649" mass="72151" checksum="23653BAFEB9D4E48" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H6V9</accession>
    <name>A0A4W2H6V9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RAB41, member RAS oncogene family</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H6V9"/>
    <dbReference type="SMR" id="A0A4W2H6V9"/>
    <dbReference type="STRING" id="30522.A0A4W2H6V9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000007105.1">
        <property type="protein sequence ID" value="ENSBIXP00000029451.1"/>
        <property type="gene ID" value="ENSBIXG00000011081.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042829.1">
        <property type="protein sequence ID" value="ENSBIXP00005026539.1"/>
        <property type="gene ID" value="ENSBIXG00005005007.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163684"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15189" checksum="45B10D1547F6FBE5" modified="2019-09-18" version="1">MSLEDRIVSVHSHVTNPKHFCSLISSNIHDSIIAVVVFNITNINFFEATDKWVEDVWAERGNYIIIMLVGNKIDLNKRKDMTTTASGISSSVPMGRVSVQFSSVPQSCPTLCNPMDCSASGFPVHHQLPELAQNHVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HBB8</accession>
    <name>A0A4W2HBB8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mab-21 like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MAB21L2</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027369228.1">
        <property type="nucleotide sequence ID" value="XM_027513427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HBB8"/>
    <dbReference type="SMR" id="A0A4W2HBB8"/>
    <dbReference type="STRING" id="30522.A0A4W2HBB8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004571.1">
        <property type="protein sequence ID" value="ENSBIXP00000006031.1"/>
        <property type="gene ID" value="ENSBIXG00000006881.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054110.1">
        <property type="protein sequence ID" value="ENSBIXP00005027078.1"/>
        <property type="gene ID" value="ENSBIXG00005005193.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113875055"/>
    <dbReference type="CTD" id="10586"/>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="OMA" id="WDESCIA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010172">
        <property type="term" value="P:embryonic body morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000002">
        <property type="entry name" value="protein mab-21-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.90:FF:000001">
        <property type="entry name" value="protein mab-21-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF37">
        <property type="entry name" value="PROTEIN MAB-21-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="7">
        <location>
            <begin position="62"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="8">
        <location>
            <begin position="246"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="359" mass="40907" checksum="24B7A0CF0E78A1AA" modified="2019-09-18" version="1">MIAAQAKLVYQLNKYYTERCQARKAAIAKTIREVCKVVSDVLKEVEVQEPRFISSLSEIDARYEGLEVISPTEFEVVLYLNQMGVFNFVDDGSLPGCAVLKLSDGRKRSMSLWVEFITASGYLSARKIRSRFQTLVAQAVDKCSYRDVVKMIADTSEVKLRIRERYVVQITPAFKCTGIWPRSAAQWPMPHIPWPGPNRVAEVKAEGFNLLSKECYSLTGKQSSAESDAWVLQFGEAENRLLMGGCRNKCLSVLKTLRDRHLELPGQPLNNYHMKTLLLYECEKHPRETDWDEACLGDRLNGILLQLISCLQCRRCPHYFLPNLDLFQGKPHSALESAAKQTWRLAREILTNPKSLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HCL6</accession>
    <name>A0A4W2HCL6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Dedicator of cytokinesis 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DOCK3</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DOCK family.</text>
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        <property type="gene ID" value="ENSBIXG00000000707.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005009413.1">
        <property type="protein sequence ID" value="ENSBIXP00005029937.1"/>
        <property type="gene ID" value="ENSBIXG00005009930.1"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHR2_DOCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="dedicator of cytokinesis protein 4"/>
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        <property type="entry name" value="Dedicator of cytokinesis protein 4"/>
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        <property type="entry name" value="DHR-2_Lobe_A"/>
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        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="67"/>
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        <location>
            <begin position="421"/>
            <end position="599"/>
        </location>
    </feature>
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        <location>
            <begin position="1223"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1636"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1729"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1844"/>
            <end position="1922"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1972"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1674"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1729"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1750"/>
            <end position="1760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1875"/>
            <end position="1897"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2009"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005029937.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="2025" mass="232513" checksum="0D9D34CD27BD6028" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HCM6</accession>
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        <submittedName>
            <fullName evidence="13">MLLT6, PHD finger containing</fullName>
        </submittedName>
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        <name evidence="13" type="primary">MLLT6</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
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        <property type="component" value="Chromosome 19"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:renal sodium excretion"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="protein AF-10 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="protein AF-10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
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        <property type="entry name" value="EPHD"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
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        <property type="entry name" value="PHD FINGER PROTEINS"/>
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    <sequence length="1095" mass="112205" checksum="9AD34202D44849A4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HXI1</accession>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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    <name>A0A4W2I2Y5_BOBOX</name>
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    <reference evidence="11 12 13" key="1">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="3">Belongs to the CELF/BRUNOL family.</text>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:translation initiation factor binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM2_CELF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12638">
        <property type="entry name" value="RRM3_CELF1_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034196">
        <property type="entry name" value="CELF1/2_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034198">
        <property type="entry name" value="CELF1/2_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034199">
        <property type="entry name" value="CELF1/2_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="43"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="135"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="429"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005038248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="514" mass="55030" checksum="AB8AF6C40645442A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IFK1</accession>
    <name>A0A4W2IFK1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Translocon-associated protein subunit alpha</fullName>
            <shortName evidence="12">TRAP-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SSR1</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 12">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma. Interacts with palmitoylated calnexin (CALX), the interaction is required for efficient folding of glycosylated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TRAP-alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027381020.1">
        <property type="nucleotide sequence ID" value="XM_027525219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IFK1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013093.1">
        <property type="protein sequence ID" value="ENSBIXP00000027528.1"/>
        <property type="gene ID" value="ENSBIXG00000008397.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038708.1">
        <property type="protein sequence ID" value="ENSBIXP00005042894.1"/>
        <property type="gene ID" value="ENSBIXG00005026495.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113882048"/>
    <dbReference type="CTD" id="6745"/>
    <dbReference type="GeneTree" id="ENSGT00400000022103"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612122" description="Translocon-associated protein subunit alpha" evidence="14">
        <location>
            <begin position="21"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="291" mass="32714" checksum="C29D670F1083D145" modified="2019-09-18" version="1">MSSLRRLLLLLLLVFPATLLLRVGPGGSLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLAEDKEEDDVSGEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTIIEREDGLDGETIFMYMFLAGLGLLVVVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQIMQSRRDKASPRRLPRKRAQKRSVGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BJU8</accession>
    <name>A0A4W2BJU8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inter-alpha-trypsin inhibitor heavy chain H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ITIH1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (HC) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of ITIH1/HC1, ITIH2/HC2 and bikunin. Interacts with TNFAIP6 (via Link and CUB domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITIH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BJU8"/>
    <dbReference type="STRING" id="30522.A0A4W2BJU8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053870.1">
        <property type="protein sequence ID" value="ENSBIXP00000000075.1"/>
        <property type="gene ID" value="ENSBIXG00000030289.1"/>
    </dbReference>
    <dbReference type="OMA" id="QEFRTTC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01461">
        <property type="entry name" value="vWA_interalpha_trypsin_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000013">
        <property type="entry name" value="inter-alpha-trypsin inhibitor heavy chain H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010600">
        <property type="entry name" value="ITI_HC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF106">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06668">
        <property type="entry name" value="ITI_HC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
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        <location>
            <begin position="27"/>
            <end position="910"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="165"/>
        </location>
    </feature>
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        <location>
            <begin position="291"/>
            <end position="474"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037051"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="910" mass="101653" checksum="62CEDD9C713836FF" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BK13</accession>
    <name>A0A4W2BK13_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase</fullName>
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            <fullName evidence="8">RING-type E3 ubiquitin transferase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="11" type="primary">MGRN1</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="8">E3 ubiquitin ligase.</text>
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        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
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        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="MGRN1/RNF157-like"/>
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        <property type="entry name" value="MGRN1/RNF157-like_N"/>
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        <property type="entry name" value="Znf_RING"/>
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    </dbReference>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MGRN1"/>
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    </dbReference>
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        <source>
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    </evidence>
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            <dbReference type="RuleBase" id="RU369081"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000153.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="585" mass="63867" checksum="A19412C5939A7328" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BKG9</accession>
    <name>A0A4W2BKG9_BOBOX</name>
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        <submittedName>
            <fullName evidence="6">Transmembrane protein 225B</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">TMEM225B</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36477:SF2">
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        <property type="match status" value="1"/>
    </dbReference>
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        </location>
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    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="221" mass="25009" checksum="AF1A26A0B94DB79D" modified="2019-09-18" version="1">MLTIEDRDVKGFTWAIAPALTSLGYLLILLVSIFPFWVRLVNEESQEVFFSGLFENCFHIKCWKPRPLSIYVILGRVSLLSAVVLSFLTTFIMVSFASQLFPRTRKHNFVSAFISFLTGACAFLALLLHALEIQSLRMKPSPPQFSIQWPYYVLGFSILLFMVAGAICLIQEIACPRCHLLPISQSTEDTQEISYLENLDSLGGELSSMQKETLLKEETII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BMN6</accession>
    <name>A0A4W2BMN6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2BMN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014613.1">
        <property type="protein sequence ID" value="ENSBIXP00000001172.1"/>
        <property type="gene ID" value="ENSBIXG00000028859.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="116"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="292"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="561" mass="65125" checksum="278B0EA33B2352B8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BNV2</accession>
    <name>A0A4W2BNV2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 740</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BNV2"/>
    <dbReference type="SMR" id="A0A4W2BNV2"/>
    <dbReference type="STRING" id="30522.A0A4W2BNV2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013454.1">
        <property type="protein sequence ID" value="ENSBIXP00000001680.1"/>
        <property type="gene ID" value="ENSBIXG00000008231.1"/>
    </dbReference>
    <dbReference type="OMA" id="PHTEIRM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000460">
        <property type="entry name" value="Putative zinc finger protein 740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000042">
        <property type="entry name" value="Zinc finger protein 148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000230">
        <property type="entry name" value="Zinc finger protein 148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF155">
        <property type="entry name" value="EARLY GROWTH RESPONSE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="33"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="193" mass="21832" checksum="5FF7919D2B7738ED" modified="2019-09-18" version="1">MAQASLLACEGLAGVSLVPTAASKKMMLSQIASKQAENGERAGSPDVLRCSSQGHRKDSDKSRSRKDDDSLAEASHSKKTVKKVVVVEQNGSFQVKIPKNFVCEHCFGAFRSSYHLKRHILIHTGEKPFECDICDMRFIQKYHLERHKCVHSGEKPYQCERCHQCFSRTDRLLRHKRMCQGFQSKTSDGQFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BP07</accession>
    <name>A0A4W2BP07_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Toll-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TOLLIP</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates. In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tollip family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027387220.1">
        <property type="nucleotide sequence ID" value="XM_027531419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BP07"/>
    <dbReference type="SMR" id="A0A4W2BP07"/>
    <dbReference type="Ensembl" id="ENSBIXT00000013344.1">
        <property type="protein sequence ID" value="ENSBIXP00000001735.1"/>
        <property type="gene ID" value="ENSBIXG00000000065.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038994.1">
        <property type="protein sequence ID" value="ENSBIXP00005023937.1"/>
        <property type="gene ID" value="ENSBIXG00005026629.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113885785"/>
    <dbReference type="CTD" id="54472"/>
    <dbReference type="GeneTree" id="ENSGT00390000013104"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04016">
        <property type="entry name" value="C2_Tollip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14363">
        <property type="entry name" value="CUE_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000036">
        <property type="entry name" value="Toll-interacting protein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000055">
        <property type="entry name" value="Toll-interacting protein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="35"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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            <dbReference type="ARBA" id="ARBA00053704"/>
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            <dbReference type="ARBA" id="ARBA00072084"/>
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        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="273" mass="30082" checksum="E74C60B1CADE8B9D" modified="2019-09-18" version="1">MATTVSTQRGPVYIGELPQDFLRITPTQQQQQIQLDAQAAQQLQYGGALGTVGRLSVTVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIQCTVPPGVDSFYLEIFDERAFSMDDRIAWTHVTIPEALKQGKVVDEWYSLSGRQGDDKEGMINLVLSYTSLPAAMMMPPQPVVLMPTVYQQGVGYVPITGMPTVCSPGVVPVALPPAVSAQPRCSEEDLKAIQDMFPNMDREVIRSVLEAQRGSRDAAINSLLQMGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQD7</accession>
    <name>A0A4W2BQD7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
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            <end position="805"/>
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        <location>
            <begin position="64"/>
            <end position="87"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="805" mass="93089" checksum="ACC435F7934C72FD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQH9</accession>
    <name>A0A4W2BQH9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQH9"/>
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        <property type="gene ID" value="ENSBIXG00000008739.1"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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            <begin position="40"/>
            <end position="224"/>
        </location>
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            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002258.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="382" mass="44287" checksum="3CDED257316A13EE" modified="2019-09-18" version="1">MNQGKLEVVKQEMARVNVNILGNSQLKWNGMGEFNSDDHYIYYCGQESLIRNGVAIMVNRRVQNTVLGCNLKNDRMISVHFQGKRLNITVIQANAPTSNAEEAEVERFYEDLQDLLELTPKKDVLFIIGDWNAKVGSQERPGITGKFGLGIRNEAGQRLIEFCQENTLVIANTLFQQHKRRLYTWTSPDGQHQNQIDYILCSQRWRSSIQSAKTRPGADCGSDHELLIAKFRLKLKKIGKTTRPFRYDLNQIPYDYTVEVRNIFKGLDLIDRVPDELWTEVHDILQETGIKTIPVEKKCKKAKWLSEDALQIAVKRREAKSKGEKERYKHLNAKFQRIARRDKKAFLSDQCKEIEENNRIGKTRDRFKKLRDTKGTFHAKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BR65</accession>
    <name>A0A4W2BR65_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ASL</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24021"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:24022"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer. Forms tissue-specific complexes with ASS1, SLC7A1, HSP90AA1 and nitric oxide synthase NOS1, NOS2 or NOS3; the complex maintenance is independent of ASL catalytic function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027383117.1">
        <property type="nucleotide sequence ID" value="XM_027527316.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027383118.1">
        <property type="nucleotide sequence ID" value="XM_027527317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BR65"/>
    <dbReference type="SMR" id="A0A4W2BR65"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014221.1">
        <property type="protein sequence ID" value="ENSBIXP00000001358.1"/>
        <property type="gene ID" value="ENSBIXG00000028661.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042072.1">
        <property type="protein sequence ID" value="ENSBIXP00005026055.1"/>
        <property type="gene ID" value="ENSBIXG00005028474.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883525"/>
    <dbReference type="CTD" id="435"/>
    <dbReference type="GeneTree" id="ENSGT00950000183122"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00273"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000014">
        <property type="entry name" value="Os03g0824900 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0835">Urea cycle</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="14">
        <location>
            <begin position="11"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="15">
        <location>
            <begin position="368"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="473" mass="52743" checksum="DCF05530CFB06404" modified="2019-09-18" version="1">MASESGKLWGGRFVGTVDPIMEKFNSSITYDRHLWEADVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNPNDEDIHTANERRLKELIGETAGKLHTGRSRNDQVVTDLRLWMRQNCSMLSALLCELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELDFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYGTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHRENMGRALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVSHVWDYGHSVEQYEALGGTARSSVDWQIGQLRALLRAQQTESPPHASPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BT91</accession>
    <name>A0A4W2BT91_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BT91"/>
    <dbReference type="STRING" id="30522.A0A4W2BT91"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012508.1">
        <property type="protein sequence ID" value="ENSBIXP00000002140.1"/>
        <property type="gene ID" value="ENSBIXG00000000163.1"/>
    </dbReference>
    <dbReference type="OMA" id="KFEQHIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000060">
        <property type="term" value="P:positive regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008530">
        <property type="entry name" value="CCDC22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048348">
        <property type="entry name" value="CCDC22_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048349">
        <property type="entry name" value="CCDC22_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668">
        <property type="entry name" value="JM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05667">
        <property type="entry name" value="CCDC22_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21674">
        <property type="entry name" value="CCDC22_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC22 N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC22 coiled-coil" evidence="5">
        <location>
            <begin position="122"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="417"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="594" mass="66750" checksum="AEAD460AF4F505E5" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BTN1</accession>
    <name>A0A4W2BTN1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig mu chain C region</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BTN1"/>
    <dbReference type="STRING" id="30522.A0A4W2BTN1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009852.1">
        <property type="protein sequence ID" value="ENSBIXP00000003430.1"/>
        <property type="gene ID" value="ENSBIXG00000000447.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000009855.1">
        <property type="protein sequence ID" value="ENSBIXP00000003433.1"/>
        <property type="gene ID" value="ENSBIXG00000000447.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd16093">
        <property type="entry name" value="IgC1_CH2_Mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05768">
        <property type="entry name" value="IgC1_CH3_IgAGD_CH4_IgAEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000998">
        <property type="entry name" value="Immunoglobulin heavy constant epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000463">
        <property type="entry name" value="Immunoglobulin heavy constant gamma 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001836">
        <property type="entry name" value="Immunoglobulin heavy constant mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="59"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="168"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="285"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="384"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="508" mass="55968" checksum="05EFD3EE03CB8746" modified="2019-09-18" version="1">MVSLWRLEMGWGPRRLEPRQAPEVFVGRGWRYHHCVYYGIDAWGRGLRVTVSSEGESLPRVFPLVSCMSSPSDESTVALGCLAQDFMPNSVSFSWKFNNSTVGSERFWTFPAVLRDGLWSASSQVVLPSSSAFQGPDDYLVCEVQHPKGGKTVGTVRVVTPKAEVLSPIVSVFVPPRNSLSGDGNSKSSLICQATDFSPKQISLSWFRDGKRIVSGISEGQVETVQSSPITFRAYSMLTITEKDWLSQNVYTCQVEHNKETFQKNVSSSCNVAPPSPIGVFTIPPSFADIFLTKSAKLSCLVTNLASYDGLNISWSRQNGKALETHTYFGRHLNDTFSARGEASVCSEDWESGEEFTCTVAHSDLPFPEKNTVSKPKDVAMKPPSVYLLPPTREQLSLRESASVTCLVKGFAPADVFVQWLQRGEPVTKSKYVTSSPAPEPQDPSVYFVHSILTVAEEDWSKGETYTCVVGHEALPHMVTERTVDKSTGKPTLYNVSLVLSDTASTCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BXN6</accession>
    <name>A0A4W2BXN6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BXN6"/>
    <dbReference type="STRING" id="30522.A0A4W2BXN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008993.1">
        <property type="protein sequence ID" value="ENSBIXP00000003877.1"/>
        <property type="gene ID" value="ENSBIXG00000010260.1"/>
    </dbReference>
    <dbReference type="OMA" id="DEYNGFG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000139">
        <property type="entry name" value="zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000555">
        <property type="entry name" value="Zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000907">
        <property type="entry name" value="Zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001530">
        <property type="entry name" value="Zinc finger protein 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 672-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="17">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="408" mass="47853" checksum="9E59520D4786473E" modified="2019-09-18" version="1">MNKSQGSVSFTDVTVDFTQEEWEQLDPSQRILYMDVMLENYSNLLSVEVWKADDQMETDPRNPDEQARPFVIFKDQTPTEERDNNLFGKTFNLSTDFVSLRQVPYKYDLYEKTLRYNSDLLTSNRNYIRKKADDCNGFGKALFYLKQEKTHPGVEYSEYNKSGKAFSHKEAIFKHQKIRNLVQPFICNYCDKAFSFKSLLISHKRIHTGEKPYECNVCKKTFSHKANLIKHQRIHTGEKPFECLECGKAFTHQSNLIVHQRAHMEKKPYECSECGKTFAQKFELTTHQRIHTGERPYECNECAKTFFKKSNLIIHQKIHTGEKRYECSECGKSFIQNSQLIIHMRTHTGEKPYECTECGKTFSQRSTLRLHLRIHTGEKPYECAECGKAFSRKSRLSVHQRVHTGDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYB0</accession>
    <name>A0A4W2BYB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYB0"/>
    <dbReference type="STRING" id="30522.A0A4W2BYB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000008428.1">
        <property type="protein sequence ID" value="ENSBIXP00000004147.1"/>
        <property type="gene ID" value="ENSBIXG00000010504.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="1">
        <location>
            <begin position="10"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="61" mass="6901" checksum="C72010E3609D6056" modified="2019-09-18" version="1">GSLPRPGRAVKKLLENMQSDLRALSLECKKKFPPVKEVSSGRLREKARTEVRAVSRFSKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1A1</accession>
    <name>A0A4W2C1A1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase 7</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Deubiquitinating enzyme 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Herpesvirus-associated ubiquitin-specific protease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquitin thioesterase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Ubiquitin-specific-processing protease 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">USP7</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2C1A1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053960.1">
        <property type="protein sequence ID" value="ENSBIXP00000000037.1"/>
        <property type="gene ID" value="ENSBIXG00000030340.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03772">
        <property type="entry name" value="MATH_HAUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000057">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000064">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000005">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF761">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="23">
        <location>
            <begin position="52"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="198"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00077310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1085" mass="126089" checksum="B36E035F77EDDCBB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C3S5</accession>
    <name>A0A4W2C3S5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C3S5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000004244.1">
        <property type="protein sequence ID" value="ENSBIXP00000006191.1"/>
        <property type="gene ID" value="ENSBIXG00000012313.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000039">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056524">
        <property type="entry name" value="KIF6/9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF67">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23735">
        <property type="entry name" value="KIF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="5"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="490"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="695"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="635"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
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        <ligand>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006191.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="794" mass="90069" checksum="028AE17D7D12DAFA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C400</accession>
    <name>A0A4W2C400_BOBOX</name>
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        <recommendedName>
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        <name evidence="13" type="primary">SSH2</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="8 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
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            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </feature>
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    </feature>
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        </location>
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        </location>
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        </location>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001034.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
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    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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    </feature>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    <sequence length="408" mass="46851" checksum="4B789DDB14D7310B" modified="2019-09-18" version="1">MLKILQARLQQYVNHELPDVQAGLRKGTGTRDQIASIHWIMEKAREFQKNIYFCFIDYAKASDCVDHKKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILPPCLFNLYAEYIMRNVGLEETQTGIKIAGRNINNLRYADDTTLMAESEEELKSLLIKVKVESEKVGLKLNIQKTKIMASGPITSWEIDGETVKTVSDFIFGGSKITADGDCSHEIKRRLLLGRKVMTNLDSILKSRDINLPTKVCLVKAMVFPVVMYGCESWTVKKAERRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGISLEEMMLELKLQYFGHLMPRADSLEKTLMLGGIGGRRRRGRQRMRWLDGITDSMDVSLSELRELVMDRNAWRAAIHEVTKSRT</sequence>
</entry>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSBIXP00000008752.1"/>
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        <property type="component" value="Chromosome 21"/>
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    </dbReference>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2595" mass="291144" checksum="81589EC19B74E9F6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7L7</accession>
    <name>A0A4W2C7L7_BOBOX</name>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
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    </dbReference>
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        <source>
            <dbReference type="RuleBase" id="RU004560"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007573.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8Z5</accession>
    <name>A0A4W2C8Z5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="7">BCAS3 domain-containing protein</fullName>
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        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268:SF0">
        <property type="entry name" value="BCAS3 MICROTUBULE ASSOCIATED CELL MIGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
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        <location>
            <begin position="716"/>
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    </feature>
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        <location>
            <begin position="472"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="934"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="103620" checksum="D7207EA1D4F7B461" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9G3</accession>
    <name>A0A4W2C9G3_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kinesin family member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2C9G3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043659.1">
        <property type="protein sequence ID" value="ENSBIXP00000008500.1"/>
        <property type="gene ID" value="ENSBIXG00000014324.1"/>
    </dbReference>
    <dbReference type="OMA" id="WLYPLTE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01372">
        <property type="entry name" value="KISc_KIF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000025">
        <property type="entry name" value="kinesin-like protein KIF27 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF8">
        <property type="entry name" value="KINESIN FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="33"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="469"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="625"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1236"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="507"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="726"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1199"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1368" mass="153815" checksum="DE6E0B97BBC9FD2E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CKE8</accession>
    <name>A0A4W2CKE8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Neurobeachin like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NBEAL1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2CKE8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022060.1">
        <property type="protein sequence ID" value="ENSBIXP00000012251.1"/>
        <property type="gene ID" value="ENSBIXG00000017346.1"/>
    </dbReference>
    <dbReference type="OMA" id="IVFKVME"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF115">
        <property type="entry name" value="NEUROBEACHIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16057">
        <property type="entry name" value="DUF4800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="6">
        <location>
            <begin position="1883"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="5">
        <location>
            <begin position="1991"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2487"/>
            <end position="2528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1382"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1386"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2693" mass="306421" checksum="C0BD87AB941BE8F0" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CL71</accession>
    <name>A0A4W2CL71_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC113905012</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CL71"/>
    <dbReference type="STRING" id="30522.A0A4W2CL71"/>
    <dbReference type="Ensembl" id="ENSBIXT00000022762.1">
        <property type="protein sequence ID" value="ENSBIXP00000012694.1"/>
        <property type="gene ID" value="ENSBIXG00000017745.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15951">
        <property type="entry name" value="7tmA_OR52R_52L-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF96">
        <property type="entry name" value="OLFACTORY RECEPTOR 52L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="57"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="329" mass="36306" checksum="9DEBF1DF138E3CF4" modified="2025-10-08" version="2">MTLIDNTEIQPLMTTLSNSSWRLIEPSFFLIGIPGLEESQHWIALPLCVLYLLALLGNVTILFTIWTDPSLHQPMYLFLAMLSGIDLVLASSTAPKTLAVLLVHAHEIGYTVCLIQMFFIHAFSSMESGVLVAMALDRYVAICHPLHHSTILHPRVIGRIGMAVLVRGILLLLPFPILLRRLVFCQATVIGHAYCEHMAVVKLSCSDSTVNRAYGLAVALLVVGLDVLAIGVSYALILQAVLKVPGGEARHKAFSTCGSHICVILVFYVPGIFSFLTHRFGRQVPHHVHVLLATLYLLVPPALNPLVYGVKTQQIRQRVLRVFDIKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CMF7</accession>
    <name>A0A4W2CMF7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2CMF7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001732.1">
        <property type="protein sequence ID" value="ENSBIXP00000007494.1"/>
        <property type="gene ID" value="ENSBIXG00000006262.1"/>
    </dbReference>
    <dbReference type="OMA" id="VDINAPH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="3080" mass="320781" checksum="D4AD9CC311ECDA91" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRG5</accession>
    <name>A0A4W2CRG5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">KICSTOR subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KICS2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the KICSTOR complex composed of KPTN, ITFG2, KICS2 and SZT2. SZT2 probably serves as a link between the other three proteins in the KICSTOR complex and may mediate the direct interaction with the GATOR complex via GATOR1. The KICSTOR complex interacts directly with the GATOR1 complex and most probably indirectly with the GATOR2 complex in an amino acid-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the KICS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRG5"/>
    <dbReference type="SMR" id="A0A4W2CRG5"/>
    <dbReference type="STRING" id="30522.A0A4W2CRG5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027680.1">
        <property type="protein sequence ID" value="ENSBIXP00000015793.1"/>
        <property type="gene ID" value="ENSBIXG00000020159.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005009276.1">
        <property type="protein sequence ID" value="ENSBIXP00005003967.1"/>
        <property type="gene ID" value="ENSBIXG00005000724.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009583"/>
    <dbReference type="OMA" id="PQKFINA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140007">
        <property type="term" value="C:KICSTOR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061462">
        <property type="term" value="P:protein localization to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3450.30:FF:000001">
        <property type="entry name" value="KICSTOR complex protein C12orf66 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038060">
        <property type="entry name" value="C12orf66-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018544">
        <property type="entry name" value="KICS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31581">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN C12ORF66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31581:SF1">
        <property type="entry name" value="KICSTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09404">
        <property type="entry name" value="C12orf66_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160651">
        <property type="entry name" value="FLJ32549 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158548">
        <property type="entry name" value="FLJ32549 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0458">Lysosome</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="50295" checksum="D47C92429BE85101" modified="2019-09-18" version="1">MGESIPLAAPVPVEQAVLETFFSHLGIFSYDKAKDNVEKEREANKSAGGSWLSLLAALAHLAAAEKVYHSLTYLGQKLGGQSFFSRKDSIRTIYTSLHNELKKVVTGRGAFGGTAPHVEELLSHLSEQLCFFVQARTEIADFYEKMYTLSTQKFINAEELVGLLDTILKKYSSRFHHPILSPLESSFQLEVDVLSHLLKAQAQVSEWKFLPSLVNLHSAHTKLQTWGQIFEKQRETKKHLFGGQSQKAVQPPHLFLWLMKLKNMLLAKFSFYFHEALSRQTTASEMKALTAKASPDLFGKISSFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVMHWPNVIMIMTDRTSDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFISFLSEISLALKNPKVFASLKPGSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRZ6</accession>
    <name>A0A4W2CRZ6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase I subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">POLR1E</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CRZ6"/>
    <dbReference type="SMR" id="A0A4W2CRZ6"/>
    <dbReference type="STRING" id="30522.A0A4W2CRZ6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025802.1">
        <property type="protein sequence ID" value="ENSBIXP00000014742.1"/>
        <property type="gene ID" value="ENSBIXG00000003439.1"/>
    </dbReference>
    <dbReference type="OMA" id="DVYPFDE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="419" mass="47053" checksum="AA603A22E19CE698" modified="2019-09-18" version="1">MASEVFPSARWQYCGEPDDSQSALLVQFSNGRLQNPGNMRFTLYKNNDSTNPRKRSQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDESVESESTPESQAKSFREKMDSCIEAFGTTKQKRALNSRRMNKVGSESLNRAVAKAAENIIDTKGVTALVSDAIHDDLQDDSVCLPPCHADAAKPEDVYKFEDLLSPVEYDALQSPSEAFRNVTSEEILKMIEENSHCSFVIEALKSLPSNEESRDRQARCIWYLDALIKFRAQKVIKRKSALGPGVPHIINTKLLKQFTCLTYNNGSLRNLISDSMKAKITAYVIVLALHINGFQIDLTMLQRDLKLSEKRMMEIAKAMRLKISKRKVSLAAGGEEEHRLGTLSIPLPPAQSLDRQSKRRKMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CSA1</accession>
    <name>A0A4W2CSA1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RBBP8 N-terminal like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RBBP8NL</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CSA1"/>
    <dbReference type="STRING" id="30522.A0A4W2CSA1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000027900.1">
        <property type="protein sequence ID" value="ENSBIXP00000016134.1"/>
        <property type="gene ID" value="ENSBIXG00000020402.1"/>
    </dbReference>
    <dbReference type="OMA" id="RCQVTQE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019518">
        <property type="entry name" value="CtIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033316">
        <property type="entry name" value="RBBP8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107:SF3">
        <property type="entry name" value="RBBP8 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107">
        <property type="entry name" value="RETINOBLASTOMA BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10482">
        <property type="entry name" value="CtIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA endonuclease Ctp1 N-terminal" evidence="3">
        <location>
            <begin position="38"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="395"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="641" mass="68994" checksum="D0247BA41ADE7F13" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CTX2</accession>
    <name>A0A4W2CTX2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Guanine nucleotide-binding protein subunit beta-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gbeta5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Transducin beta chain 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GNB5</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, such as RGS7 and RGS9, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation. Increases RGS7 GTPase-activating protein (GAP) activity, thereby regulating mood and cognition. Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors. May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a complex composed of RGS9 (isoform RGS9-1), GNB5 and RGS9BP; within this complex, the presence of GNB5 stabilizes both itself and RGS9 and increases RGS9 GTPase-activating protein (GAP) activity. Interacts with RGS7, forming the RGS7-GNB5 complex; within this complex, the presence of GNB5 increases RGS7 GTPase-activating protein (GAP) activity. Interacts with GPR158; promotes the GTPase activator activity of the RGS7-GNB5 complex in absence of glycine, in contrast GTPase activator activity of the RGS7-GNB5 complex is inhibited in presence of glycine. Interacts with RGS6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CTX2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000015283.1"/>
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    <sequence length="394" mass="43515" checksum="15689DB008ACE701" modified="2019-09-18" version="1">MDQTFLVNVFGSCDRCFKQRALRPVFKKSEQLNYCSLCVEIMAADGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRLVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHESDINSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CV66</accession>
    <name>A0A4W2CV66_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="STRING" id="30522.A0A4W2CV66"/>
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        <property type="protein sequence ID" value="ENSBIXP00000015824.1"/>
        <property type="gene ID" value="ENSBIXG00000003703.1"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Alpha_kinase_ChaK2_TRPM6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transient receptor potential cation channel subfamily M member 7"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MHCK/EF2 kinase"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="a-kinase_dom"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="TRPM"/>
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        <property type="entry name" value="TRPM-like"/>
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    <dbReference type="InterPro" id="IPR029597">
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    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
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    <dbReference type="InterPro" id="IPR032415">
        <property type="entry name" value="TRPM_tetra"/>
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        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF15">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16519">
        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="20 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="759"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="855"/>
            <end position="874"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
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            <begin position="922"/>
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    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="952"/>
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    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="991"/>
            <end position="1011"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="1064"/>
            <end position="1087"/>
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    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="31">
        <location>
            <begin position="1762"/>
            <end position="1992"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="593"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
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    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2013"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="1501"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="1624"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="1682"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="2025"/>
            <end position="2034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00025760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2034" mass="232170" checksum="4A8E5CD98AC4738B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CVX4</accession>
    <name>A0A4W2CVX4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein archease</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein ZBTB8OS</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB. Together with DDX1, acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the tRNA-splicing ligase complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archease family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CVX4"/>
    <dbReference type="SMR" id="A0A4W2CVX4"/>
    <dbReference type="STRING" id="30522.A0A4W2CVX4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047293.1">
        <property type="protein sequence ID" value="ENSBIXP00000016074.1"/>
        <property type="gene ID" value="ENSBIXG00000020370.1"/>
    </dbReference>
    <dbReference type="OMA" id="AITYHKM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.10.10:FF:000001">
        <property type="entry name" value="protein archease isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="9">
        <location>
            <begin position="31"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="167" mass="19432" checksum="124DF476591C13E1" modified="2019-09-18" version="1">MALEGEDVRDYNLTEEQKAIKAKYPPVSRKYEYLDHTADVQLHAWGDTLEEAFEQCAMAMFGYMTDTGTVEPLQTIEVETQGDDLQSLLFHFLDEWLYKFSADEFFIPREVKVLNIDQRNFKIRSIGWGEEFSLSKHPQGTEVKAITYSAMQVYNEEKPEVFVIIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXE2</accession>
    <name>A0A4W2CXE2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="40">Multifunctional protein CAD</fullName>
            <ecNumber evidence="10">2.1.3.2</ecNumber>
            <ecNumber evidence="9">3.5.1.2</ecNumber>
            <ecNumber evidence="8">3.5.2.3</ecNumber>
            <ecNumber evidence="32">6.3.4.16</ecNumber>
            <ecNumber evidence="7">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="41">Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="46" type="primary">CAD</name>
    </gene>
    <organism evidence="46 47">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="46 47 48" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="46" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="38">Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="39">Homohexamer. Interacts with CIPC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="31">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="28">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="29">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="30">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="10"/>
    <dbReference type="EC" id="3.5.1.2" evidence="9"/>
    <dbReference type="EC" id="3.5.2.3" evidence="8"/>
    <dbReference type="EC" id="6.3.4.16" evidence="32"/>
    <dbReference type="EC" id="6.3.5.5" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027411631.1">
        <property type="nucleotide sequence ID" value="XM_027555830.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A4W2CXE2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030915.1">
        <property type="protein sequence ID" value="ENSBIXP00000018084.1"/>
        <property type="gene ID" value="ENSBIXG00000004252.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043156.1">
        <property type="protein sequence ID" value="ENSBIXP00005045159.1"/>
        <property type="gene ID" value="ENSBIXG00005005081.1"/>
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        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01979">
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATCASE"/>
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    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
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        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
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        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="PROSITE" id="PS00482">
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
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    </dbReference>
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        <property type="entry name" value="MGS"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="21 42" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0436">Ligase</keyword>
    <keyword evidence="22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="25" id="KW-0464">Manganese</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18 42" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="47" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ATP-grasp" evidence="44">
        <location>
            <begin position="519"/>
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    <feature type="domain" description="ATP-grasp" evidence="44">
        <location>
            <begin position="1052"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="45">
        <location>
            <begin position="1308"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="43">
        <location>
            <begin position="1815"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="43">
        <location>
            <begin position="1866"/>
            <end position="1878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1258" mass="139145" checksum="49D9BC644333CC22" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D139</accession>
    <name>A0A4W2D139_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="primary">LOC113896981</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the alpha/beta interferon family.</text>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interferon alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
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        <property type="entry name" value="INTERFERON OMEGA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INTERFERONAB"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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        </location>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="193" mass="21891" checksum="A3ACC2A5E90A1F93" modified="2019-09-18" version="1">MAVMLSLLMALVLVSYGLGGSLGCDLSQNHMLVGRKNLRLLGQMRRLSPRFCLQDRKDFAFPQEVVEGSQLQEAQAFSVLHEMLQQTFNLFNTEHSSAAWDTTLLEPLHTGLHQQLDDLDACLGQVMGEEDSALGRTGPTLAMKRYFHGIHVYLREKEYSDCAWEIVRLEIMRSLSSSTNLQERLRMMDGDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D294</accession>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
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        <lineage>
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            <taxon>Ruminantia</taxon>
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    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D3S9</accession>
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        <submittedName>
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            <taxon>Pecora</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="LEUCINE-RICH REPEAT, IMMUNOGLOBULIN-LIKE DOMAIN AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021507537" evidence="8">
        <location>
            <begin position="21"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="253"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="509"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000018874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="549" mass="60903" checksum="21792972D29D900D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4H9</accession>
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        <recommendedName>
            <fullName evidence="16">Isoleucine--tRNA ligase, cytoplasmic</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="21" type="primary">IARS1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.</text>
    </comment>
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        <reaction evidence="14">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000020762.1"/>
        <property type="gene ID" value="ENSBIXG00000024043.1"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
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        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
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        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin_IARS1"/>
    </dbReference>
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        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
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        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="18">
        <location>
            <begin position="17"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="19">
        <location>
            <begin position="693"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="Isoleucine--tRNA ligase cytoplasmic ubiquitin-like" evidence="20">
        <location>
            <begin position="1077"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="Isoleucine--tRNA ligase cytoplasmic ubiquitin-like" evidence="20">
        <location>
            <begin position="1169"/>
            <end position="1259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF23567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1262" mass="144642" checksum="A36736D55E15CB98" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D597</accession>
    <name>A0A4W2D597_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF24">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 8-4"/>
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    </dbReference>
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        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="112" mass="12582" checksum="200DF68043AF2AC7" modified="2019-09-18" version="1">AQLVTQPDGHINVSEGNHVELRCNYSYGGPIYLFWYVQYPNQGLQFLLKYLSGPTRVKGIKGFEAEFKKDENSFHLMKASAHWSDSAKYFCALCDTMPESAGGAEQKPSQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D6T1</accession>
    <name>A0A4W2D6T1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113896308</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D6T1"/>
    <dbReference type="STRING" id="30522.A0A4W2D6T1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050071.1">
        <property type="protein sequence ID" value="ENSBIXP00000021627.1"/>
        <property type="gene ID" value="ENSBIXG00000024668.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="21"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="294" mass="33135" checksum="2FC6EE8E6B44FC64" modified="2019-09-18" version="1">PSLEMTLFVTVSISYMVTLMGNTTIILLFHLDPHLHTPMYFFLTNLSLLDLCFTTSCIPQMLVHLRGPDKTISYLGCAVQLYIFLGLGAAECVLLLVMAFDRYVAVCRPLHYTVIMQPRVCHKLAFLVWTSGIFGTLVQSPTTMKLPFCHHHRVDDFVCEVPALIRLACGDTHSNELQISVIGSVFLMVPLLLILISYGHIARTVLAIQSHEGRRKAFQTCSSHLVVVFLFYCTVTAVYIQPRSHFSQKGGKFLTLFYTVVTPTLNPLIYTLRNKDMKGALKRLFGKDRTSNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D8Z3</accession>
    <name>A0A4W2D8Z3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D8Z3"/>
    <dbReference type="STRING" id="30522.A0A4W2D8Z3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046898.1">
        <property type="protein sequence ID" value="ENSBIXP00000015226.1"/>
        <property type="gene ID" value="ENSBIXG00000019779.1"/>
    </dbReference>
    <dbReference type="OMA" id="NWVKQAV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000012">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF108">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4 9" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021514468" description="C-C motif chemokine" evidence="9">
        <location>
            <begin position="27"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="10">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="96" mass="10904" checksum="65F3075342A0AE33" modified="2019-09-18" version="1">MCSSKNLLLAALMSVLLLHFCSKSEAASNFDCCLRYTERILHPSILVGFTQQLANEACDINAVVFYTRKKLAVCADPKKKWVKQVVHMLSQRVKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D9N9</accession>
    <name>A0A4W2D9N9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 170</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D9N9"/>
    <dbReference type="STRING" id="30522.A0A4W2D9N9"/>
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        <property type="protein sequence ID" value="ENSBIXP00000020893.1"/>
        <property type="gene ID" value="ENSBIXG00000024123.1"/>
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    <dbReference type="OMA" id="DCETGQD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039139">
        <property type="entry name" value="CCDC170-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18863:SF5">
        <property type="entry name" value="TESTIS EXPRESSED GENE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18863">
        <property type="entry name" value="TSEC-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="76"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="67747" checksum="D8C65AFAD3923183" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DAT6</accession>
    <name>A0A4W2DAT6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phospholipase A2</fullName>
            <ecNumber evidence="11">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73008"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:40816"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2DAT6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050839.1">
        <property type="protein sequence ID" value="ENSBIXP00000023164.1"/>
        <property type="gene ID" value="ENSBIXG00000025720.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000001">
        <property type="entry name" value="Basic phospholipase A2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF8">
        <property type="entry name" value="GROUP IIF SECRETORY PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="13">
        <location>
            <begin position="137"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="162"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="179"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="185"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="186"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="271" mass="29482" checksum="3957373CA6FA041E" modified="2019-09-18" version="1">MEGAGSDVRPGPILQDREPSVSWKPGSGGREGGSLCLPALHCWGLLLPEVQAGRRCSDVSAGANHRARATGTRAPRGGVDCGCKEAQEGAIGQEPHAVGRDRLRTGLSSPLRSHLGMKSFITITILASSVLPAARSSLLNLKSMVETVTGRNAILSFVGYGCYCGLGGRGLPMDEVDWCCHAHDCCYQKLFDLGCRTYVDHYDYTIENDTDIVCSELNQTECDKQTCECDRSVVLCLQKQTYREEHRNYLNIYCQGPTPNCSIYEPPPVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DE68</accession>
    <name>A0A4W2DE68_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rho guanine nucleotide exchange factor 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <text evidence="10">May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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    <sequence length="584" mass="65166" checksum="2087B3E4EF7A2693" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <begin position="1"/>
            <end position="27"/>
        </location>
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        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
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            <begin position="125"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="135" mass="14027" checksum="3348EB39FEF66F2A" modified="2025-10-08" version="2">MGFTGPHTPSPGGAEHRSAVYSSSESTNRLDTAQMAMIRYTFTSTNAVSAPAAPDNAREEALGAWPLSSSDSSTVAVAVSAGSPWSFTSTSRRWRGASASSRARVVLISPVYSPTRNGLRAPSAASCSSYDSHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PSPC family.</text>
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        <property type="term" value="C:nuclear matrix"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of circadian rhythm"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="79"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="153"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="457"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="280"/>
            <end position="348"/>
        </location>
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        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020221"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="520" mass="58275" checksum="42297292FE0C2A60" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DLZ7</accession>
    <name>A0A4W2DLZ7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DLZ7"/>
    <dbReference type="STRING" id="30522.A0A4W2DLZ7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000033709.1">
        <property type="protein sequence ID" value="ENSBIXP00000019909.1"/>
        <property type="gene ID" value="ENSBIXG00000023368.1"/>
    </dbReference>
    <dbReference type="OMA" id="HQCGETW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000287">
        <property type="entry name" value="PR domain zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002876">
        <property type="entry name" value="Zgc:172128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000770">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000204">
        <property type="entry name" value="Zinc finger protein 331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000165">
        <property type="entry name" value="Zinc finger protein 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF143">
        <property type="entry name" value="ENDOTHELIAL ZINC FINGER PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="328"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="411" mass="47230" checksum="8D3AFA187D2D1530" modified="2019-09-18" version="1">MFLDIGGHQTLERDWRNHLVEKLCIDNEGHHCLEALNQITDLTLHKGYWPGIQPCKCTKCGKAYRGCSFQNQQKSLPGYRHYPCEECGEACSCVSCLSPPRGTDIVEEPNKCQDAGRTCKRCVKSHGSKWSSDCKKSGKALAFPSSFKGHERGQHGQKTHTCKVCGKSFSYHSYLARHMRTHTEEKHCECEECEQALRYSLSLVGHMTIHTSDKAYMCKKCGKAFSCPKYFRRHMKTHSGMKPYECTECGKAYSSSLSLREHAGTHSQEKPYECQHCGKAFRHQRYFKKHVKMHNGAKPYKCTECGKAYSCSMSLQEHERTHTGEKPFGCPFCGKAFMHQASLRGHLRTHSRERAYTCTQCQKAFRWPSSLRKHVRMHSEEKPYACQQCGKAFWYPTNLRGHMRTHTGEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP51</accession>
    <name>A0A4W2DP51_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DP51"/>
    <dbReference type="STRING" id="30522.A0A4W2DP51"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010898.1">
        <property type="protein sequence ID" value="ENSBIXP00000028246.1"/>
        <property type="gene ID" value="ENSBIXG00000005943.1"/>
    </dbReference>
    <dbReference type="OMA" id="NTKGMFG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006907">
        <property type="term" value="P:pinocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07663">
        <property type="entry name" value="BAR_SNX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000008">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000001">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028654">
        <property type="entry name" value="BAR_SNX5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014637">
        <property type="entry name" value="SNX5/SNX6/SNX32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850">
        <property type="entry name" value="SORTING NEXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850:SF5">
        <property type="entry name" value="SORTING NEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036924">
        <property type="entry name" value="Snx5_Snx6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="376" mass="43675" checksum="68E90A5AF26BD29C" modified="2019-09-18" version="1">MALYIDVPDALSERDKVKFTVHTKTTLPTFQSPEFSVTRQHEDCVWLHDTLIETTDYAGLIIPPAPTKPDFDGPREKMQKLGEGEGSMTKEEFAKMKQELEAEYLAVFRKTVSSHEVFLQRFSPHPVLSKDRNFHVFLEYDQDLSVRRKNTKEVFGGFFKSVVKSADEVLFSGVKEVDDFFEQEKTFLINYYNRIKDSCAKADRMTRSHKNVADDYIHTAACLHSLALEEPTIIKKYLLKVAELFEKLRKVESRVSSDEDLKLTELLRYYMLNIEAAENLLYRRTKALIDYESSNKALDKARLKSKDVKLAEAHQQECCQKFEQLSESAKDELINFKRKRVAAFRKNLIEMSELEIKHARNNVSLLQSCIDLFKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DP87</accession>
    <name>A0A4W2DP87_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">YEATS domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">YEATS2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Chromatin reader component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at 'Lys-27' (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, SGF29 and DR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="SMR" id="A0A4W2DP87"/>
    <dbReference type="STRING" id="30522.A0A4W2DP87"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034992.1">
        <property type="protein sequence ID" value="ENSBIXP00000020744.1"/>
        <property type="gene ID" value="ENSBIXG00000024028.1"/>
    </dbReference>
    <dbReference type="OMA" id="SACKNFA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16907">
        <property type="entry name" value="YEATS_YEATS2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1970:FF:000001">
        <property type="entry name" value="YEATS domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055127">
        <property type="entry name" value="YEATS2_3HBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22951">
        <property type="entry name" value="3HBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YEATS" evidence="13">
        <location>
            <begin position="201"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="117"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="337"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="512"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="793"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="954"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1094"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="798"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="956"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000020744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1412" mass="149921" checksum="B16EA824A17C419A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DS62</accession>
    <name>A0A4W2DS62_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GTF2I repeat domain containing 2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DS62"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014705.1">
        <property type="protein sequence ID" value="ENSBIXP00000027050.1"/>
        <property type="gene ID" value="ENSBIXG00000028913.1"/>
    </dbReference>
    <dbReference type="OMA" id="XETEVEV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000002">
        <property type="entry name" value="General transcription factor II-I isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000003">
        <property type="entry name" value="general transcription factor II-I isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1460.10">
        <property type="entry name" value="GTF2I-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004212">
        <property type="entry name" value="GTF2I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036647">
        <property type="entry name" value="GTF2I-like_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042224">
        <property type="entry name" value="GTF2IRD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47831">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47831:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02946">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117773">
        <property type="entry name" value="GTF2I-like repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51139">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="50356" checksum="5B4D1FD0B32A1AD6" modified="2019-09-18" version="1">MAQVAVSTPPIAHEESSESRMVVTFLVSALESMCKELAKSKAEVACIAMYEADVFVIGTEKGRAFVNARTDLQKDFAKYCVAEGLQEVKPPGPANASRMQVDSGETEILRKAVEDYFCFCYGKALGTAAMVPVPYEKMLTDQEAIVVQGLPEGVPFQHPENYDLATLKWILENKGGISFLINRPFPGPANQPGGPGVVTDTDKSVTSPSESCTPIRVKTEPMEDSGISLKAEVVSVKKESEDPNYYEYSMQESRHSSAGTEVIETELPMEDSTQLVPSETSEDPEAEVKIEGNTSSPNITNSAAGVEDLNIIQVTVPDNEKERLSSLEKIKQLREQVNDLFSRKFGEAIGVDFPVKVPYRKITFNPGCVVIDGMPPGVVFKAPGYLEISSMRRILDAADLIKFTVIRPLPGLELSNGKNAGVGCHFLLQGIFPTQGSNLSLLCLLHCKQILYLLNHQGSPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DS67</accession>
    <name>A0A4W2DS67_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">LIM and cysteine-rich domains protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LMCD1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with beta-dystroglycan. Interacts with GATA1, GATA4 and GATA6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027378317.1">
        <property type="nucleotide sequence ID" value="XM_027522516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DS67"/>
    <dbReference type="SMR" id="A0A4W2DS67"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="363" mass="40723" checksum="7115263766A64F39" modified="2019-09-18" version="1">MAKVAKDLNPRVQKMSLGQQQSARGVPCLRCKGTCSGFEPHSWRKICKSCKCSQEDHCLSSDLEDDRKIGRLLMDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGALYRRRQLMHQLPIYDQDPSRCRGLLENELKVMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETAPPTTNGSIGDPSKEYVCELCKGVAPADSPVVYSDRAGYSKQWHPACFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFSEDYQRVEDLAWHRKHFVCEGCEQQLGGRAYIVTMGQLLCPTCSKSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DUH7</accession>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
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            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10470"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000028135.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="71" mass="7773" checksum="0F1775E061970741" modified="2019-09-18" version="1">MVFAPGEKPGSEPEEAKLQTTSRQIVQNAILRAVQQVSRESQSRRRDARAGDTARGSLRPGAGESTKKHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DVJ7</accession>
    <name>A0A4W2DVJ7_BOBOX</name>
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        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DVJ7"/>
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        <property type="protein sequence ID" value="ENSBIXP00000030689.1"/>
        <property type="gene ID" value="ENSBIXG00000012745.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="IgG receptor FcRn large subunit p51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF154">
        <property type="entry name" value="MHC CLASS I POLYPEPTIDE-RELATED SEQUENCE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021291775" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="23"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="201"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="375" mass="42495" checksum="83E65751EF9D6DDC" modified="2019-09-18" version="1">MGLSRVCLFLAHAAFFVLLGNAAGSHSLLYNMTVLSRDGFVQSRFFAEGFLDRQTFLHYDHKKGRAEPWGRWAEKLAAETWETESTDLNDSWKELRKLLAEILSLQEEKEGLHSLQETVGCNIDEDSHPRGFRLLYFNGELLLSCYPEPHGCTLPQSSARTLAMEMELSKHYQAHVQGELCWRLRSYLESWTGFTERTVPPAVNVTHSQDSEGMVHLTCKAFGFFPRNISVVWFRDEEPMSRDAQESGDVLPDGNGTYYTWETIRIPQGEEQRVKCIVEHSGNHSAHLIPLGKTLAHQSSWWIVSVSVAVVFIIGFCVYCYIKKRKTASATGRPEPIRLQDLDQFQTEPTDHNGLTHPEFQSLSHSGSISLTGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DXA7</accession>
    <name>A0A4W2DXA7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2 hydrogencarbonate(out) + chloride(in) + Na(+)(out) = 2 hydrogencarbonate(in) + chloride(out) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72739"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Cs(+)(in) + 2 hydrogencarbonate(in) + chloride(out) = Cs(+)(out) + 2 hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75071"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + 2 hydrogencarbonate(in) + chloride(out) = K(+)(out) + 2 hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75059"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Li(+)(in) + 2 hydrogencarbonate(in) + chloride(out) = Li(+)(out) + 2 hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75063"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Rb(+)(in) + 2 hydrogencarbonate(in) + chloride(out) = Rb(+)(out) + 2 hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75067"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DXA7"/>
    <dbReference type="STRING" id="30522.A0A4W2DXA7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051252.1">
        <property type="protein sequence ID" value="ENSBIXP00000023938.1"/>
        <property type="gene ID" value="ENSBIXG00000026275.1"/>
    </dbReference>
    <dbReference type="OMA" id="GEMPPIT"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140900">
        <property type="term" value="F:chloride:bicarbonate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000011">
        <property type="entry name" value="Anion exchange protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF52">
        <property type="entry name" value="ANION EXCHANGE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="421"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="727"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="787"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="813"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="19">
        <location>
            <begin position="54"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="19">
        <location>
            <begin position="179"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="18">
        <location>
            <begin position="358"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="920"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000023938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="957" mass="105463" checksum="A27501B2B97BA03C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZD7</accession>
    <name>A0A4W2DZD7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Casein kinase I isoform alpha</fullName>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">CK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">CSNK1A1</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30 31" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation. Interacts with FAM83A, FAM83B, FAM83C, FAM83D, FAM83E, FAM83F, FAM83G and FAM83H (via DUF1669). Interaction with FAM83H recruits CSNK1A1 to keratin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027401788.1">
        <property type="nucleotide sequence ID" value="XM_027545987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZD7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040986.1">
        <property type="protein sequence ID" value="ENSBIXP00000024699.1"/>
        <property type="gene ID" value="ENSBIXG00000026822.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027074.1">
        <property type="protein sequence ID" value="ENSBIXP00005015946.1"/>
        <property type="gene ID" value="ENSBIXG00005019760.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895174"/>
    <dbReference type="CTD" id="1452"/>
    <dbReference type="GeneTree" id="ENSGT00940000153700"/>
    <dbReference type="OMA" id="ESRVYKY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14128">
        <property type="entry name" value="STKc_CK1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000120">
        <property type="entry name" value="Casein kinase I isoform alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 25 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0137">Centromere</keyword>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="11 25 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 26" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="17"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00071374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="364" mass="41790" checksum="856FB15947D9F55B" modified="2019-09-18" version="1">MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKCLESPVGKRKRSMTVSTSQDPSFSGLNQLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZS2</accession>
    <name>A0A4W2DZS2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox B6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HOXB6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027373115.1">
        <property type="nucleotide sequence ID" value="XM_027517314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DZS2"/>
    <dbReference type="SMR" id="A0A4W2DZS2"/>
    <dbReference type="STRING" id="30522.A0A4W2DZS2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005306.1">
        <property type="protein sequence ID" value="ENSBIXP00000030021.1"/>
        <property type="gene ID" value="ENSBIXG00000011817.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014964.1">
        <property type="protein sequence ID" value="ENSBIXP00005028090.1"/>
        <property type="gene ID" value="ENSBIXG00005029554.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113877297"/>
    <dbReference type="CTD" id="3216"/>
    <dbReference type="GeneTree" id="ENSGT00940000161108"/>
    <dbReference type="OMA" id="CAQDKNV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034101">
        <property type="term" value="P:erythrocyte homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000017">
        <property type="entry name" value="Homeobox protein antennapedia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="HOMEOBOX"/>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="144"/>
            <end position="204"/>
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            <dbReference type="PROSITE" id="PS50071"/>
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    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25310" checksum="EC569964DDD4193D" modified="2019-09-18" version="1">MSSYFVNSTFPVTLASGQESFLGQLPLYSSGYADPLRHYPAPYGPGPGQDKGFAASSYYPPAGGGYGRAAPCDYGPAPAFYREKESACALSGADEPPPFHPEPRKSDCAQDKSVFGEAEEQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLELEKEFHYNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKESKLLSASQLSAEEEEEKPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZW0</accession>
    <name>A0A4W2DZW0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="RT_POL"/>
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            <begin position="77"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032051.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="526" mass="60698" checksum="52345C5658FD77DA" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E0L1</accession>
    <name>A0A4W2E0L1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-1 adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Beta-1 adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling. Involved in the regulation of sleep/wake behaviors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct. Interacts with GOPC, MAGI3 and DLG4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="Ensembl" id="ENSBIXT00000006417.1">
        <property type="protein sequence ID" value="ENSBIXP00000029665.1"/>
        <property type="gene ID" value="ENSBIXG00000011361.1"/>
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        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002025">
        <property type="term" value="P:norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045823">
        <property type="term" value="P:positive regulation of heart contraction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="7tmA_Beta1_AR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADR_fam"/>
    </dbReference>
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        <property type="entry name" value="ADRB1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF54">
        <property type="entry name" value="BETA-1 ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00561">
        <property type="entry name" value="ADRENRGCB1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 20" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 20" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 20 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="23">
        <location>
            <begin position="122"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="455"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EFA2"/>
    <dbReference type="STRING" id="30522.A0A4W2EFA2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003464.1">
        <property type="protein sequence ID" value="ENSBIXP00000030635.1"/>
        <property type="gene ID" value="ENSBIXG00000012674.1"/>
    </dbReference>
    <dbReference type="OMA" id="LCIKYEP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039884">
        <property type="entry name" value="R3HC1/R3HCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21678:SF7">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN R3HCC1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21678">
        <property type="entry name" value="GROWTH INHIBITION AND DIFFERENTIATION RELATED PROTEIN 88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="786" mass="87050" checksum="6AC24DCBF240E9ED" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGX5</accession>
    <name>A0A4W2EGX5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGX5"/>
    <dbReference type="STRING" id="30522.A0A4W2EGX5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025214.1">
        <property type="protein sequence ID" value="ENSBIXP00000035756.1"/>
        <property type="gene ID" value="ENSBIXG00000019087.1"/>
    </dbReference>
    <dbReference type="OMA" id="VWECKAL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000004">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF31">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="204"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="287"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="363"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="16"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="535" mass="58848" checksum="072E11E4A683D80E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EI14</accession>
    <name>A0A4W2EI14_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDRGK domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Component of the UFM1 ribosome E3 ligase (UREL) complex, composed of UFL1, DDRGK1 and CDK5RAP3. Interacts with (unphosphorylated) ERN1/IRE1-alpha; interaction is dependent on UFM1 and takes place in response to endoplasmic reticulum stress, regulating ERN1/IRE1-alpha stability. Interacts with NFKBIA. Interacts with SOX9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDRGK1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EI14"/>
    <dbReference type="SMR" id="A0A4W2EI14"/>
    <dbReference type="Ensembl" id="ENSBIXT00000026692.1">
        <property type="protein sequence ID" value="ENSBIXP00000036390.1"/>
        <property type="gene ID" value="ENSBIXG00000019862.1"/>
    </dbReference>
    <dbReference type="OMA" id="EFTRECN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903895">
        <property type="term" value="P:negative regulation of IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000143">
        <property type="entry name" value="DDRGK domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019153">
        <property type="entry name" value="DDRGK_dom-contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050899">
        <property type="entry name" value="DDRGK_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176:SF1">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09756">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01128">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="40"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="313" mass="35667" checksum="6E1AC35F7FA33202" modified="2019-09-18" version="1">MVSPVVYLVVAALLVGLILFLTRGRGRAAAAAQEPLHNEEVPAAAGRVARPQPLEPEEQRAAGRPRRRRDLGSRLQAQRRAQRVAWADENEEEAIIQAQEEEDIEKPVETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAQEEQAQREHEEYLKLKETFVVVEEGVGETMTEEQSHSFLAEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTLTGVIDDRGKFIYITPEELAAVANFIRQRGRVSITELAQASNSLIAWGRETPAQAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EI93</accession>
    <name>A0A4W2EI93_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein prune homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">BNIP2 motif-containing molecule at the C-terminal region 1</fullName>
        </alternativeName>
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        <name evidence="11" type="primary">PRUNE2</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="8">May play an important role in regulating differentiation, survival and aggressiveness of the tumor cells.</text>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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    <comment type="similarity">
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        <property type="entry name" value="DHHA2 DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="DHH phosphoesterases"/>
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        <property type="entry name" value="CRAL_TRIO"/>
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    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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            <title>Haplotype-resolved cattle genomes.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <text evidence="1">May be involved in transcriptional regulation.</text>
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        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000144">
        <property type="entry name" value="zinc finger protein 181 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000127">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000443">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002004">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF242">
        <property type="entry name" value="ZINC FINGER PROTEIN 628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="67494" checksum="05FEA77CBE2D7E44" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ENP8</accession>
    <name>A0A4W2ENP8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DAZ-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deleted in azoospermia-associated protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proline-rich transcript in brain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DAZAP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027398698.1">
        <property type="nucleotide sequence ID" value="XM_027542897.1"/>
    </dbReference>
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    <dbReference type="STRING" id="30522.A0A4W2ENP8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000031594.1">
        <property type="protein sequence ID" value="ENSBIXP00000038491.1"/>
        <property type="gene ID" value="ENSBIXG00000004329.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046484.1">
        <property type="protein sequence ID" value="ENSBIXP00005013057.1"/>
        <property type="gene ID" value="ENSBIXG00005017295.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893144"/>
    <dbReference type="CTD" id="9802"/>
    <dbReference type="GeneTree" id="ENSGT00390000000685"/>
    <dbReference type="OMA" id="IYQPRYM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031435">
        <property type="term" value="F:mitogen-activated protein kinase kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120283">
        <property type="term" value="F:protein serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050699">
        <property type="term" value="F:WW domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022730">
        <property type="entry name" value="DAZ_assoc-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31638">
        <property type="entry name" value="DAZ-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31638:SF4">
        <property type="entry name" value="DAZ-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11029">
        <property type="entry name" value="DAZAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000038491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="168" mass="17319" checksum="49F1B6D281E24AAC" modified="2019-09-18" version="1">MNSKGQYPTQPTYPVQPPGNPVYPQTLHLPQAPPYTDAPPAYSELYRPSFVHPGAATVPTMSAAFPGASLYLPMAQSVAVGPLGSTIPMAYYPVGPIYPPGSTVLVEGGYDAGARFGAGATAGNIPPPPPGCPPNAAQLAVMQGANVLVTQRKGNFFMGGSDGGYTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ES04</accession>
    <name>A0A4W2ES04_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HEAT repeat containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HEATR4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <location>
            <begin position="173"/>
            <end position="193"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="886"/>
            <end position="913"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039497.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1030" mass="116517" checksum="EBCEA675A96FCA7A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ES09</accession>
    <name>A0A4W2ES09_BOBOX</name>
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        <name evidence="3" type="primary">TINF2</name>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="451" mass="49796" checksum="B38FC4E2C5DE5A35" modified="2019-09-18" version="1">MATPPGAGPAALRFVAAASWQVIRGRCVEHFPRVVEFLRYLRAAAPGLVRYRHHERLCMGLKAKLVVDLILQGRPWAQVLNALHHHFPESGPVVRDPKITKQDLRKISEAQETFCQQVKQLAEAPVDLASKLQELEQEYGETFMAAMEKLFFEYLCQLEKALPTLQAQQLQDVLSWMQPGVSITSSFVLSQYGVDMGWPLPEYSATDSVNTTEPSEQSPPQQPRPALHDPLPKASPGPLLPQGPASRKHPEPLTGHHFNLAPLGRRRIQPQWAPTSRGHKERPTVMLLPFRDVGSPAQVTSKPGKSKDGTHTADAAGAVGTRAPSTGKSKSPSETLGGRALKENPTDSSASEQKENCHMDCHMEPLRLSLSPPRKSVCPPSLYSSDITIGDLVLDSDEEEDGQRERRESLENYQKTKFDTLIPTFCEYLPSSGPSTVSIPFPDCTDSSRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ET89</accession>
    <name>A0A4W2ET89_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bos</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ET89"/>
    <dbReference type="STRING" id="30522.A0A4W2ET89"/>
    <dbReference type="Ensembl" id="ENSBIXT00000035342.1">
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        <property type="gene ID" value="ENSBIXG00000024174.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="T-CELL SURFACE ANTIGEN CD2"/>
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    </dbReference>
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        <property type="entry name" value="CD2ANTIGEN"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
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            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021425756" description="Immunoglobulin V-set domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="338"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="338" mass="38248" checksum="2C58830E52E952CE" modified="2019-09-18" version="1">MNLACEILASFLLIFTVPTKGEDPESIVVWGALDHDLNLDIPGFPRSDIVADIKWNRNKNKIARIKKDMPLHNEMDKYDMFTNGTLKIKTLMRNDSGLYEVEVYDSNGVNLLSKKFDLKIQEMLSGPEINWICTNRTVSCKVENGSNPKLELFLNMTRVKQDHGKLITYTWNTRWNKTFKCVASNHVDSKVSIEIAVCPDEGLDLYLIIGICVGGAVFLLFVALLIFYISRRKKQSRRRDDEELEIKAQRAILEERGRKPQQTPVSTPANPGMSQTPPVPGHRSQPPAHRPRALGPRVQPQQKRLPPTPGTQVHQQKGPPLPKPRVQTKPPCDAEENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETQ0</accession>
    <name>A0A4W2ETQ0_BOBOX</name>
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        <recommendedName>
            <fullName evidence="10">Ubiquitin-protein ligase E3C</fullName>
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        <alternativeName>
            <fullName evidence="11">HECT-type ubiquitin transferase E3C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RTA-associated ubiquitin ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">UBE3C</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with 26S proteasomes. Interacts (via the HECT domain) with UBE2D1 and, less efficiently, with UBE2L3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UBE3C family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ETQ0"/>
    <dbReference type="SMR" id="A0A4W2ETQ0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036028.1">
        <property type="protein sequence ID" value="ENSBIXP00000040413.1"/>
        <property type="gene ID" value="ENSBIXG00000004944.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000011">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000014">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="746"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1085" mass="123385" checksum="2A076493548B35D6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EU87</accession>
    <name>A0A4W2EU87_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EU87"/>
    <dbReference type="STRING" id="30522.A0A4W2EU87"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036452.1">
        <property type="protein sequence ID" value="ENSBIXP00000040608.1"/>
        <property type="gene ID" value="ENSBIXG00000024703.1"/>
    </dbReference>
    <dbReference type="OMA" id="ISWLTHI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:muscle alpha-actinin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:heart development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
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        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24262" checksum="1B70AD7F8EC7A305" modified="2019-09-18" version="1">IDSFKVVLEGPALWGFWPQVGKDFTVPFSISWLTHIDGENASSSMAVGDWVLSIDGENAWGLIHMEAQNKIHACGESLSLGLSRAQSPQRKQQKALAPRHGPPLPTPTPAQAVHLCTQCFPQQYTWPSGQPRSLTVPCSRMDSCLASSGKTMSWSCRAHATPTSRTGTPGTLPMCSTCNGSLALAGWLPPLPLFLFFHLGHLPLALPASAYLTHPFVAPALSLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUW6</accession>
    <name>A0A4W2EUW6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microfibrillar-associated protein 3-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EUW6"/>
    <dbReference type="STRING" id="30522.A0A4W2EUW6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050244.1">
        <property type="protein sequence ID" value="ENSBIXP00000040804.1"/>
        <property type="gene ID" value="ENSBIXG00000024954.1"/>
    </dbReference>
    <dbReference type="OMA" id="CVLIDCN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001040">
        <property type="entry name" value="Microfibrillar-associated protein 3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
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        <property type="match status" value="1"/>
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            <begin position="1"/>
            <end position="28"/>
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            <begin position="29"/>
            <end position="401"/>
        </location>
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            <begin position="148"/>
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            <begin position="316"/>
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            <begin position="331"/>
            <end position="340"/>
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            <begin position="353"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00067552"/>
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            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040804.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="401" mass="43405" checksum="32BAA7903E41DF78" modified="2019-09-18" version="1">MDGVKRHLPVCFLPPVPLLILVSTLATASSVTSSTLNGTNVAVGLAPVVVARTDHIIVKEGNSALINCSVSGLPEPQFKWYNSAGKLLSENEEDQGGGKWQMHDGLLNITKVSFSDRGRYTCMASNLHGSVNNTVTLRVVFTSGDMGVYYMVVCLVAFAVVLVLNVTRLCMMSSHLKKTEKAINEFFRTEGAEKLQKAFEIAKRIPIITSAKTLELAKVTQFKTMEFARYIEELARSVPLPPLIMNCRTIVEELMEVVGLDEHGHQNFVRHTPEGQEAAAADGDEVYTIPDADSDASSLHEQPQQIAIEVSVHPPAHREPSDDRAAGALEAGDEEEDTEPSAEHSPESAAPSTEVTSAALTSEEPTPVEVPDHVLPPGLLEAAEPARPRDGSTHVVYESHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EUX2</accession>
    <name>A0A4W2EUX2_BOBOX</name>
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        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EUX2"/>
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            <end position="80"/>
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        </location>
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    </feature>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="840" mass="89883" checksum="4B484CEBDC8F2421" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EX32</accession>
    <name>A0A4W2EX32_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Xin repeats bind F-actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Xin family.</text>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EX32"/>
    <dbReference type="Ensembl" id="ENSBIXT00000038074.1">
        <property type="protein sequence ID" value="ENSBIXP00000041343.1"/>
        <property type="gene ID" value="ENSBIXG00000025593.1"/>
    </dbReference>
    <dbReference type="OMA" id="MPLYAIQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012510">
        <property type="entry name" value="Actin-binding_Xin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030072">
        <property type="entry name" value="XIRP1/XIRP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22591">
        <property type="entry name" value="XIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22591:SF1">
        <property type="entry name" value="XIN ACTIN-BINDING REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08043">
        <property type="entry name" value="Xin"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51389">
        <property type="entry name" value="XIN"/>
        <property type="match status" value="26"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="540"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="575"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="615"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="652"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="690"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="730"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="768"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="806"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="840"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="874"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="911"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1016"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1054"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1093"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1126"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1164"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1199"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1238"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1274"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1311"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1349"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1386"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1420"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1451"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1489"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="repeat" description="Xin" evidence="5">
        <location>
            <begin position="1524"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="393"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2114"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2266"/>
            <end position="2294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2331"/>
            <end position="2368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2388"/>
            <end position="2525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2541"/>
            <end position="2565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2736"/>
            <end position="2755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2792"/>
            <end position="2817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3068"/>
            <end position="3167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3359"/>
            <end position="3379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3502"/>
            <end position="3532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1916"/>
            <end position="1943"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="14"/>
            <end position="23"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <end position="145"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="162"/>
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            <begin position="407"/>
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            <begin position="2279"/>
            <end position="2290"/>
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            <end position="2365"/>
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            <end position="2397"/>
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            <end position="2435"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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    </feature>
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        <location>
            <begin position="2457"/>
            <end position="2473"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <begin position="2482"/>
            <end position="2494"/>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <end position="3095"/>
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        <location>
            <begin position="3103"/>
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            <begin position="3522"/>
            <end position="3532"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00721"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041343.1"/>
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    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="3532" mass="400807" checksum="E3185309616DE4A3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EXY6</accession>
    <name>A0A4W2EXY6_BOBOX</name>
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        <recommendedName>
            <fullName evidence="8">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transposase 22 family.</text>
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        <property type="gene ID" value="ENSBIXG00000026129.1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="LINE-1 retrotransposable element ORF1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L1 transposable element, trimerization domain"/>
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    </dbReference>
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        <property type="entry name" value="L1_C"/>
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    <dbReference type="InterPro" id="IPR035300">
        <property type="entry name" value="L1_dsRBD"/>
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        <property type="entry name" value="L1_RRM_dom"/>
    </dbReference>
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        <property type="entry name" value="L1_trimer"/>
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    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
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    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tnp_22_dsRBD"/>
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            <begin position="54"/>
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            <dbReference type="Pfam" id="PF17489"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="30996" checksum="A6E7B165B1C98641" modified="2019-09-18" version="1">MESQINSLETRIEKMQERFNKDLEEIKKSQYIMNNAINEIKNTLEATNSRITEAEDRISELEDRMVEISESERIKEKRIKRNEDNLRDLQDNIKPYNIQIIGVPEEEDKKKDHEKILEEIIVENFPKMGKEIITQVQETQRVPNRINPRRNTPRHILIKLTKIKHKEQILKAAREKQQITHKGIPIRITADLSIETLQARREWQDILKMMKENNLQPRLLYPARISFKYEGEIKSFSDKQKLREFCTTKPALQQTLKDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EYQ2</accession>
    <name>A0A4W2EYQ2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2EYQ2"/>
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        <property type="protein sequence ID" value="ENSBIXP00000041901.1"/>
        <property type="gene ID" value="ENSBIXG00000005365.1"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SP4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039938">
        <property type="entry name" value="Sp4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947:SF23">
        <property type="entry name" value="SP4 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="646"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="676"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="706"/>
            <end position="733"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="109"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="279"/>
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            <begin position="37"/>
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            <begin position="118"/>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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        </location>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="793" mass="83281" checksum="4C8AFDD0A9F77D2B" modified="2019-09-18" version="1"> </sequence>
</entry>
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    <accession>A0A4W2EYW2</accession>
    <name>A0A4W2EYW2_BOBOX</name>
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            <fullName evidence="4">Large ribosomal subunit protein bL34m</fullName>
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        </alternativeName>
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        <name evidence="6" type="primary">MRPL34</name>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Artiodactyla</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000041958.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="96" mass="10754" checksum="19A6A46ACA49B9CA" modified="2019-09-18" version="1">MAFSVGSVGCLLGPVSRSAGLLGGRWLQGSRAWLGLPDTRRLPVIQQTRGRTRGNEYQPSNIKRKNKHGWIRRLSTPNGVQVILRRMHKGRKSLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0W1</accession>
    <name>A0A4W2F0W1_BOBOX</name>
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        <submittedName>
            <fullName evidence="2">Retroelement silencing factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RESF1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2F0W1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000042676.1"/>
        <property type="gene ID" value="ENSBIXG00000026624.1"/>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990226">
        <property type="term" value="F:histone methyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027866">
        <property type="entry name" value="RESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21604">
        <property type="entry name" value="RETROELEMENT SILENCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21604:SF0">
        <property type="entry name" value="RETROELEMENT SILENCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15395">
        <property type="entry name" value="DUF4617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="834"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1112"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1421"/>
            <end position="1519"/>
        </location>
    </feature>
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        <location>
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        <location>
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        <location>
            <begin position="1463"/>
            <end position="1503"/>
        </location>
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        <source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1741" mass="193848" checksum="EE2D1A9B83B27754" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F8E1</accession>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="45"/>
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        </location>
    </feature>
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            <begin position="1"/>
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        </location>
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        <location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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    <name>A0A4W2F961_BOBOX</name>
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    <reference evidence="6 7 8" key="1">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the secretoglobin family.</text>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Secretoglobin_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005001361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10167" checksum="3F2EFD481D036DB7" modified="2019-09-18" version="1">MKGALLVLALMVTRELTFKTHEACPVFYETVFAVSLGLPRKALNETLDSVDANEAEKAAFGKLWDCFIEAEPEDRFNHLKYKVTYLYPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FPQ1</accession>
    <name>A0A4W2FPQ1_BOBOX</name>
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        <submittedName>
            <fullName evidence="8">Thioredoxin</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="synonym">LOC113897645</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
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            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0702">S-nitrosylation</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="7"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="4">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="4">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="4">
        <location>
            <position position="34"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019748.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9541" checksum="E40006FBC111887E" modified="2019-09-18" version="1">MVKQIESKYAFQEALNSAGEKLVVVDFSATWCGPCKMIKPFFHDVAAECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2FPY6</accession>
    <name>A0A4W2FPY6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Clarin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CLRN2</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the clarin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027401214.1">
        <property type="nucleotide sequence ID" value="XM_027545413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2FPY6"/>
    <dbReference type="STRING" id="30522.A0A4W2FPY6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049297.1">
        <property type="protein sequence ID" value="ENSBIXP00000039638.1"/>
        <property type="gene ID" value="ENSBIXG00000023547.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005002269.1">
        <property type="protein sequence ID" value="ENSBIXP00005007496.1"/>
        <property type="gene ID" value="ENSBIXG00005005831.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894746"/>
    <dbReference type="CTD" id="645104"/>
    <dbReference type="GeneTree" id="ENSGT00850000132319"/>
    <dbReference type="OMA" id="PKWMTGK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032421">
        <property type="term" value="C:stereocilium bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120045">
        <property type="term" value="P:stereocilium maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026748">
        <property type="entry name" value="Clarin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548">
        <property type="entry name" value="CLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548:SF5">
        <property type="entry name" value="CLARIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25807">
        <property type="entry name" value="Clarin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005007496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="232" mass="25490" checksum="3CC0F5D1B7B5CD48" modified="2019-09-18" version="1">MPGWFKKAWYGLASLLSFSSFILIIVALALPHWLSGKILCQTGVDLVNATDPELVKFIGDIYYGLFRGCKVRQCGLGGRQSQFTIFPHLVKELNAGLHVTLLLLLFLALALALVSMGFAILNMIQVPYRAVNGPGGICLWNVLAGGVVALAIASFMTSVKFHDLTERIANFREKLFRFVVVEEQYEESFWICVASASAHATNLVVVAISQIPLPEVQAKIEEATVTAEDILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G0N2</accession>
    <name>A0A4W2G0N2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Extended synaptotagmin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ESYT1</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Acts as an inhibitor of ADGRD1 G-protein-coupled receptor activity in absence of cytosolic calcium. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with ESYT2 and ESYT3. Interacts with ADGRD1; inhibiting the G-protein-coupled receptor activity of ADGRD1. Interaction with ADGRD1 is abolished when cytosolic calcium increases, relieving ADGRD1 G-protein-coupled receptor activity. Interacts (phosphorylated form) with SLC2A4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the extended synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2G0N2"/>
    <dbReference type="STRING" id="30522.A0A4W2G0N2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043397.1">
        <property type="protein sequence ID" value="ENSBIXP00000044214.1"/>
        <property type="gene ID" value="ENSBIXG00000028401.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005018760.1">
        <property type="protein sequence ID" value="ENSBIXP00005010389.1"/>
        <property type="gene ID" value="ENSBIXG00005015035.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156561"/>
    <dbReference type="OMA" id="KVHPGQS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120014">
        <property type="term" value="F:phospholipid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08391">
        <property type="entry name" value="C2A_C2C_Synaptotagmin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04050">
        <property type="entry name" value="C2B_Synaptotagmin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04030">
        <property type="entry name" value="C2C_KIAA1228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21679">
        <property type="entry name" value="SMP_ESyt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000025">
        <property type="entry name" value="Extended synaptotagmin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000106">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000120">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000124">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000139">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037752">
        <property type="entry name" value="C2C_KIAA1228"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037733">
        <property type="entry name" value="Ext_Synaptotagmin_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037749">
        <property type="entry name" value="Ext_Synaptotagmin_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051634">
        <property type="entry name" value="Extended_Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761:SF3">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="15" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="23">
        <location>
            <begin position="139"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="316"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="458"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="642"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="790"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="983"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00093580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005010389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1116" mass="124172" checksum="87D2F4C022B742F9" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2G2M6</accession>
    <name>A0A4W2G2M6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 300</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CFAP300</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium- and flagellum-specific protein that plays a role in axonemal structure organization and motility. May play a role in outer and inner dynein arm assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CFAP300 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027419729.1">
        <property type="nucleotide sequence ID" value="XM_027563928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2G2M6"/>
    <dbReference type="STRING" id="30522.A0A4W2G2M6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005631.1">
        <property type="protein sequence ID" value="ENSBIXP00000005506.1"/>
        <property type="gene ID" value="ENSBIXG00000007137.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005021213.1">
        <property type="protein sequence ID" value="ENSBIXP00005012033.1"/>
        <property type="gene ID" value="ENSBIXG00005002602.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905933"/>
    <dbReference type="CTD" id="85016"/>
    <dbReference type="GeneTree" id="ENSGT00510000047559"/>
    <dbReference type="OMA" id="FYHCYGV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029416">
        <property type="entry name" value="CFAP300"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14926">
        <property type="entry name" value="CFAP300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005012033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="267" mass="30721" checksum="C977589C080FC211" modified="2019-09-18" version="1">MAAGEPGDLGVYSFRFLPQKTFQSLSTPQTTSRLRQWSMLGRIEAQAFGFDQTFQAYRKDDFVMAFFKDPNVIPNLKLLSESSGEWLTLGTEVKKIEAINVPCTQLSMSFFNRLYDEAIVRDNGYIVKCLDSFCDPFLISDELRKVLLVEDSEKYEVFSQPEREEFLFCLFKHLCLGGALCQYEDVINPYLETTKLIYKDLVSVRKNPQTKKIQITSSIFKVTAYDSVGVCYPSTKSHEQTFSYFIVDPIKRHVHVLYHCYGMGEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GIH8</accession>
    <name>A0A4W2GIH8_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dickkopf WNT signaling pathway inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DKK1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384973.1">
        <property type="nucleotide sequence ID" value="XM_027529172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GIH8"/>
    <dbReference type="SMR" id="A0A4W2GIH8"/>
    <dbReference type="STRING" id="30522.A0A4W2GIH8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001090.1">
        <property type="protein sequence ID" value="ENSBIXP00000007801.1"/>
        <property type="gene ID" value="ENSBIXG00000013693.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005031082.1">
        <property type="protein sequence ID" value="ENSBIXP00005018620.1"/>
        <property type="gene ID" value="ENSBIXG00005021979.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113884540"/>
    <dbReference type="CTD" id="22943"/>
    <dbReference type="GeneTree" id="ENSGT00940000161319"/>
    <dbReference type="OMA" id="LDNYQPY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050750">
        <property type="term" value="F:low-density lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000726">
        <property type="term" value="P:negative regulation of cardiac muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042662">
        <property type="term" value="P:negative regulation of mesodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060392">
        <property type="term" value="P:negative regulation of SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904723">
        <property type="term" value="P:negative regulation of Wnt-Frizzled-LRP5/6 complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042663">
        <property type="term" value="P:regulation of endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002090">
        <property type="term" value="P:regulation of receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23272">
        <property type="entry name" value="Dkk1_Cys2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23026">
        <property type="entry name" value="Dkk1_N_Cys1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000001">
        <property type="entry name" value="Dickkopf WNT-signaling pathway inhibitor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048500">
        <property type="entry name" value="DIKK1/2/4_C-subdom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048499">
        <property type="entry name" value="DIKK1/2/4_C-subdom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047304">
        <property type="entry name" value="Dkk1_Cys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047305">
        <property type="entry name" value="Dkk1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF11">
        <property type="entry name" value="DICKKOPF-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21481">
        <property type="entry name" value="DIKK1-2-4_C-subdom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21479">
        <property type="entry name" value="DIKK1-2-4_C-subdom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="8">
        <location>
            <begin position="85"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf-related protein 1/2/4 C-terminal subdomain 1" evidence="10">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf-related protein 1/2/4 C-terminal subdomain 2" evidence="9">
        <location>
            <begin position="216"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005018620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="265" mass="28624" checksum="A198FF88FED75378" modified="2019-09-18" version="1">MTALGTAGAARVLVALVAAALCGHPLLGVSATLNFVLNSNAIKNLPRPLGGAAGHPGSAVSAAPGIPFEGGSKYQTIDNHQPYPCAEDEECSTDEYCASPTRGAGAQICLACRKLRKRCMRHAMCCPGNHCKNGICMPSDQNHFHRGEIEETIIESFGNEHSTLDGYSRRTTLSSRMYHTKGQEGSACLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCQIQKDHHQASNSSRLHTCQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GL87</accession>
    <name>A0A4W2GL87_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC113904789</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027417679.1">
        <property type="nucleotide sequence ID" value="XM_027561878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GL87"/>
    <dbReference type="STRING" id="30522.A0A4W2GL87"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045584.1">
        <property type="protein sequence ID" value="ENSBIXP00000012432.1"/>
        <property type="gene ID" value="ENSBIXG00000017505.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032601.1">
        <property type="protein sequence ID" value="ENSBIXP00005019647.1"/>
        <property type="gene ID" value="ENSBIXG00005003899.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113904789"/>
    <dbReference type="GeneTree" id="ENSGT01150000286940"/>
    <dbReference type="OMA" id="CRFQLYH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF98">
        <property type="entry name" value="OLFACTORY RECEPTOR 52A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005019647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="316" mass="35980" checksum="D3657E228DB52409" modified="2019-09-18" version="1">MSIYNTTVFMPSEFTLIGIPGLESVQCWIGIPFCAMYLMAMIGNSLLLIIIKSEHSLHQPMYIFLGMLGVTDIALSTSIVPKMLGIFWFHVTEIYFDSCLLQMWLIHTFQGIESDILLAMALDRYVAICYPLRHAAIFSHQLVTQIAAMVTLRAAVLVAPCLVLIKFRLQFYHTTVISHSYCEHMAVVKLVAENIRVNKIYGLFVAFTVAGLDIVLITLSYIQIFITVFRLPQKEARLKAFNTCVAHICVFLQFYSLGFFSFFAHRFGSHIPPYIHILFSSTYLLVPPFLNPLVYGAKTKQIRVHMIKIFCSKNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GY77</accession>
    <name>A0A4W2GY77_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RHBDF1</name>
        <name evidence="16" type="synonym">POLR3K</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Core component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol III active site to constantly monitor transcription fidelity, cleaves mis-incorporated 5'-ribonucleotides and restarts the transcription process. Once Pol III reaches the poly(dT) termination signal, can induce Pol III clamp opening and transcription termination. Pol III plays an important role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 14">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2GY77"/>
    <dbReference type="SMR" id="A0A4W2GY77"/>
    <dbReference type="STRING" id="30522.A0A4W2GY77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000014342.1">
        <property type="protein sequence ID" value="ENSBIXP00000027148.1"/>
        <property type="gene ID" value="ENSBIXG00000028716.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005038122.1">
        <property type="protein sequence ID" value="ENSBIXP00005023340.1"/>
        <property type="gene ID" value="ENSBIXG00005026144.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156278"/>
    <dbReference type="OMA" id="MEFCDEC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="5"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044007"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005023340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="108" mass="12316" checksum="3D651776179726B8" modified="2019-09-18" version="1">MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNVTRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAEPCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2GZD8</accession>
    <name>A0A4W2GZD8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">La-related protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">La ribonucleoprotein domain family member 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LARP7</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Core component of the 7SK RNP complex, at least composed of 7SK RNA, LARP7, MEPCE, HEXIM1 (or HEXIM2) and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1). Interacts with METTL16. Interacts with RBM7; upon genotoxic stress this interaction is enhanced, triggering the release of inactive P-TEFb complex from the core, yielding to P-TEFb complex activation. Associates with box C/D small nucleolar ribonucleoprotein (snoRNP) complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LARP7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399614.1">
        <property type="nucleotide sequence ID" value="XM_027543813.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027399615.1">
        <property type="nucleotide sequence ID" value="XM_027543814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2GZD8"/>
    <dbReference type="STRING" id="30522.A0A4W2GZD8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042497.1">
        <property type="protein sequence ID" value="ENSBIXP00000043617.1"/>
        <property type="gene ID" value="ENSBIXG00000027773.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039179.1">
        <property type="protein sequence ID" value="ENSBIXP00005024068.1"/>
        <property type="gene ID" value="ENSBIXG00005026718.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113894016"/>
    <dbReference type="CTD" id="51574"/>
    <dbReference type="GeneTree" id="ENSGT00940000154949"/>
    <dbReference type="OMA" id="WCSLRNK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120259">
        <property type="term" value="C:7SK snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097322">
        <property type="term" value="F:7SK snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032897">
        <property type="term" value="P:negative regulation of viral transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904871">
        <property type="term" value="P:positive regulation of protein localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905382">
        <property type="term" value="P:positive regulation of snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990438">
        <property type="term" value="P:U6 2'-O-snRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08032">
        <property type="entry name" value="LARP_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12290">
        <property type="entry name" value="RRM1_LARP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12542">
        <property type="entry name" value="RRM2_LARP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000158">
        <property type="entry name" value="La ribonucleoprotein domain family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000281">
        <property type="entry name" value="la-related protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000379">
        <property type="entry name" value="la-related protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014886">
        <property type="entry name" value="La_xRRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034946">
        <property type="entry name" value="LARP7_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034887">
        <property type="entry name" value="LARP7_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034910">
        <property type="entry name" value="LARP7_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF62">
        <property type="entry name" value="LA-RELATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08777">
        <property type="entry name" value="RRM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51939">
        <property type="entry name" value="XRRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="21">
        <location>
            <begin position="26"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="20">
        <location>
            <begin position="123"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="XRRM" evidence="22">
        <location>
            <begin position="446"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="184"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="409"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="578" mass="65894" checksum="CCA12938C7C82B5B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H1A1</accession>
    <name>A0A4W2H1A1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H1A1"/>
    <dbReference type="STRING" id="30522.A0A4W2H1A1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000042970.1">
        <property type="protein sequence ID" value="ENSBIXP00000043920.1"/>
        <property type="gene ID" value="ENSBIXG00000028102.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039651.1">
        <property type="protein sequence ID" value="ENSBIXP00005024434.1"/>
        <property type="gene ID" value="ENSBIXG00005027013.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003710"/>
    <dbReference type="OMA" id="CFANCIY"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28632">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005024434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="88" mass="9960" checksum="BA21EA1693DCAB37" modified="2019-09-18" version="1">MSKCGKKKPLKQPRKQAKEMDQEDKTFKQKQKEEQKKLEELKAKASGKGPLARGEIKKFGRVSCSFREGNGTPLQYSCLENPMDGGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H2U7</accession>
    <name>A0A4W2H2U7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="21">SEC24 homolog D, COPII coat complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SEC24D</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399534.1">
        <property type="nucleotide sequence ID" value="XM_027543733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H2U7"/>
    <dbReference type="STRING" id="30522.A0A4W2H2U7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052590.1">
        <property type="protein sequence ID" value="ENSBIXP00000025952.1"/>
        <property type="gene ID" value="ENSBIXG00000028048.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041064.1">
        <property type="protein sequence ID" value="ENSBIXP00005025370.1"/>
        <property type="gene ID" value="ENSBIXG00005027886.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893997"/>
    <dbReference type="CTD" id="9871"/>
    <dbReference type="GeneTree" id="ENSGT00950000182924"/>
    <dbReference type="OMA" id="RLCKHGD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000023">
        <property type="entry name" value="protein transport protein Sec24C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000020">
        <property type="entry name" value="protein transport protein Sec24D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000003">
        <property type="entry name" value="SEC24 homolog D, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000004">
        <property type="entry name" value="SEC24 homolog D, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF6">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="17">
        <location>
            <begin position="360"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="18">
        <location>
            <begin position="437"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="20">
        <location>
            <begin position="686"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="19">
        <location>
            <begin position="782"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="900"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="129"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1032" mass="112487" checksum="B627725FB9F5B859" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H6Z7</accession>
    <name>A0A4W2H6Z7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptogyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SYNGR3</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027383332.1">
        <property type="nucleotide sequence ID" value="XM_027527531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H6Z7"/>
    <dbReference type="SMR" id="A0A4W2H6Z7"/>
    <dbReference type="STRING" id="30522.A0A4W2H6Z7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000015453.1">
        <property type="protein sequence ID" value="ENSBIXP00000026803.1"/>
        <property type="gene ID" value="ENSBIXG00000029373.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005000151.1">
        <property type="protein sequence ID" value="ENSBIXP00005027729.1"/>
        <property type="gene ID" value="ENSBIXG00005030188.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883642"/>
    <dbReference type="CTD" id="9143"/>
    <dbReference type="GeneTree" id="ENSGT00950000182935"/>
    <dbReference type="OMA" id="RVTTWIF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF8">
        <property type="entry name" value="SYNAPTOGYRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011282">
        <property type="entry name" value="Synaptogyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="20"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR011282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="229" mass="24499" checksum="3642ACC34A7D4F6D" modified="2019-09-18" version="1">MEGGSFGAGRAGAALDPVSFARRPQTLLRVASWVFSIAVFGPIVNEGYVNADSGPELRCVFNGNAGACRFGVALGLGAFLACSCFLLLDVRFQQISSVRDRRRAVLLDLGFSGLWSFLWFVGFCFLTNQWQRTAPGPGTAQAGDAARAVITFSFFSILSWVALTVKALQRFRLGTDMSLFATEQLGAGAGQTYPGYPVGSGVEGTDTYQSPPFTETLDTSPKGYQVPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H811</accession>
    <name>A0A4W2H811_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone deacetylase 11</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HDAC11</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with HDAC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027378535.1">
        <property type="nucleotide sequence ID" value="XM_027522734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2H811"/>
    <dbReference type="STRING" id="30522.A0A4W2H811"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011442.1">
        <property type="protein sequence ID" value="ENSBIXP00000028063.1"/>
        <property type="gene ID" value="ENSBIXG00000009136.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005041727.1">
        <property type="protein sequence ID" value="ENSBIXP00005025815.1"/>
        <property type="gene ID" value="ENSBIXG00005028341.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880507"/>
    <dbReference type="CTD" id="79885"/>
    <dbReference type="GeneTree" id="ENSGT00940000156308"/>
    <dbReference type="OMA" id="EIGFPWS"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09993">
        <property type="entry name" value="HDAC_classIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000009">
        <property type="entry name" value="Histone deacetylase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044150">
        <property type="entry name" value="HDAC_classIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF23">
        <property type="entry name" value="HISTONE DEACETYLASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="35"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005025815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="347" mass="39042" checksum="82D2F3BFABBA0EAE" modified="2019-09-18" version="1">MPHPTQLYQHVPDSRWPIVYSPRYNITFLGLEKLHPFDAGKWGKVISLLKEEKLLSDSMLVEAREASDEDLLVVHTRRYLNELKWSFAVATITEIPPVIFLPNFLVQRKVLRPLRTQTGGTIMAGKLAVDRGWAINVGGGFHHCSSDRGGGFCAYADITLAIKFLFDRVEGISKATIVDLDAHQGNGHERDFMGDKRVYIMDVYNRHIYPGDRFAKQAIRRKVELEWGTEDDEYLQKVERNLEKALQEHRPDIVVYNAGTDILEGDRLGGLAISPQGVVKRDELVFRIVRGRQLPILMVTSGGYQKRTARIIADSILNLYSLGLIGPESASVSAQNSDTPLLPPEVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2H977</accession>
    <name>A0A4W2H977_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Syntaxin-binding protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">STXBP6</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Forms non-fusogenic complexes with SNAP25 and STX1A and may thereby modulate the formation of functional SNARE complexes and exocytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a ternary complex containing SNAP25 and STX1A that can be dissociated by NAPA and NSF. Interacts with STX4A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2H977"/>
    <dbReference type="STRING" id="30522.A0A4W2H977"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018869.1">
        <property type="protein sequence ID" value="ENSBIXP00000010171.1"/>
        <property type="gene ID" value="ENSBIXG00000002342.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043181.1">
        <property type="protein sequence ID" value="ENSBIXP00005026746.1"/>
        <property type="gene ID" value="ENSBIXG00005002110.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156499"/>
    <dbReference type="OMA" id="CDPNKDC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035542">
        <property type="term" value="P:regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14681">
        <property type="entry name" value="PH-STXBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15892">
        <property type="entry name" value="R-SNARE_STXBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000029">
        <property type="entry name" value="Syntaxin-binding protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.90:FF:000002">
        <property type="entry name" value="syntaxin-binding protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037821">
        <property type="entry name" value="STXBP6_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037822">
        <property type="entry name" value="STXBP6_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="194"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005026746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="253" mass="28514" checksum="47F5BAD6D32C3A47" modified="2019-09-18" version="1">MTNPVVFSPPNICKIECRGEVTTVSRFQILNENSLRALVTWLTMSAKSAISKEIFAPLDERMLGAVQVKRRTKKKIPFLATGGQGEYLTYICLSVTNKKPTQASITKVKQFEGSTSFVRRSQWMLEQLRQVNGIDPNRDSAEFDLLFENAFDQWVASTASEKCTFFQILHHTCQRYLTDRKPEFINCQSKIMGGNSILHSAADSVTSAVQKASQALNERGERLGRAEEKTEDLKNSAQQFAETAHKLAMKHKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HBP3</accession>
    <name>A0A4W2HBP3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Caspase recruitment domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HBP3"/>
    <dbReference type="STRING" id="30522.A0A4W2HBP3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000039016.1">
        <property type="protein sequence ID" value="ENSBIXP00000023429.1"/>
        <property type="gene ID" value="ENSBIXG00000005331.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014214.1">
        <property type="protein sequence ID" value="ENSBIXP00005028340.1"/>
        <property type="gene ID" value="ENSBIXG00005007940.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000245143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031964">
        <property type="entry name" value="CARD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16739">
        <property type="entry name" value="CARD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Caspase recruitment" evidence="7">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005028340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="65" mass="7811" checksum="AAEA75F88F585D86" modified="2019-09-18" version="1">MMFAEDKTYQYICYHHSNFCNVHVLDILPHLSCLTTSDQDQFRASYERWGKQGTLWDLFDSLRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2HSC6</accession>
    <name>A0A4W2HSC6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein disulfide-isomerase-like protein of the testis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PDILT</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Probable redox-inactive chaperone involved in spermatogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027384026.1">
        <property type="nucleotide sequence ID" value="XM_027528225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2HSC6"/>
    <dbReference type="SMR" id="A0A4W2HSC6"/>
    <dbReference type="STRING" id="30522.A0A4W2HSC6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000009685.1">
        <property type="protein sequence ID" value="ENSBIXP00000003523.1"/>
        <property type="gene ID" value="ENSBIXG00000009935.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005046360.1">
        <property type="protein sequence ID" value="ENSBIXP00005035750.1"/>
        <property type="gene ID" value="ENSBIXG00005016987.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113883978"/>
    <dbReference type="CTD" id="204474"/>
    <dbReference type="GeneTree" id="ENSGT00940000160939"/>
    <dbReference type="OMA" id="APWSKKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008354">
        <property type="term" value="P:germ cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000167">
        <property type="entry name" value="Protein disulfide isomerase like, testis expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000177">
        <property type="entry name" value="Protein disulfide isomerase like, testis expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000191">
        <property type="entry name" value="Protein disulfide isomerase like, testis expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000209">
        <property type="entry name" value="Protein disulfide isomerase like, testis expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF58">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE-LIKE PROTEIN OF THE TESTIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044611942" description="Protein disulfide-isomerase-like protein of the testis" evidence="14">
        <location>
            <begin position="21"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="54"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="389"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="519"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="540"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005035750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="578" mass="66938" checksum="7103C94E30390015" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2IAF5</accession>
    <name>A0A4W2IAF5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox protein DBX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Developing brain homeobox protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DBX1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Could have a role in patterning the central nervous system during embryogenesis. Has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. Regulates the transcription factor profile, neurotransmitter phenotype, intraspinal migratory path and axonal trajectory of V0 neurons, features that differentiate them from an adjacent set of V1 neurons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the H2.0 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027388322.1">
        <property type="nucleotide sequence ID" value="XM_027532521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2IAF5"/>
    <dbReference type="SMR" id="A0A4W2IAF5"/>
    <dbReference type="STRING" id="30522.A0A4W2IAF5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012625.1">
        <property type="protein sequence ID" value="ENSBIXP00000027681.1"/>
        <property type="gene ID" value="ENSBIXG00000000139.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005049619.1">
        <property type="protein sequence ID" value="ENSBIXP00005039909.1"/>
        <property type="gene ID" value="ENSBIXG00005022387.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113886361"/>
    <dbReference type="CTD" id="120237"/>
    <dbReference type="GeneTree" id="ENSGT00950000183093"/>
    <dbReference type="OMA" id="RHSLAYH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021521">
        <property type="term" value="P:ventral spinal cord interneuron specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000177">
        <property type="entry name" value="Homeobox protein DBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051662">
        <property type="entry name" value="H2.0_Homeobox_NeuralPatt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24331">
        <property type="entry name" value="DBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24331:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN DBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="180"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="182"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="241"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005039909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="345" mass="37467" checksum="7BB2D366F61461FE" modified="2019-09-18" version="1">MMFPGLLAPPAGYPSLLRPTPTLTLPQSLQSAFSGHSSFLVEDLIRISRPPAYLPRGSVPTPSMSPPRPGAPAALTDTGASDLGSPGPGSRRGGSPQTAVSPASEPTFLKFGVNAILSSAPRTETSPTLLQSVPPKTFAFPYFEGSFQPFIRSSYFPASSSVVPIPGTFSWPLAARGKPRRGMLRRAVFSDVQRKALEKMFQKQKYISKPDRKKLAAKLGLKDSQVKIWFQNRRMKWRNSKERELLSSGGCREQTLPTKLNPHPDLSDVGQKGPGDDDDEEDEGPGSPRPRLVYHAAPADPRHLRDPRLEAPLPTSPARSGSPDKASDFSDSEDDEEGEEEITVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ICH3</accession>
    <name>A0A4W2ICH3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E-selectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">CD62 antigen-like family member E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Endothelial leukocyte adhesion molecule 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Leukocyte-endothelial cell adhesion molecule 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">SELE</name>
    </gene>
    <organism evidence="31 33">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32 33" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selectin/LECAM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027420557.1">
        <property type="nucleotide sequence ID" value="XM_027564756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ICH3"/>
    <dbReference type="SMR" id="A0A4W2ICH3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047118.1">
        <property type="protein sequence ID" value="ENSBIXP00000015672.1"/>
        <property type="gene ID" value="ENSBIXG00000020093.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005033668.1">
        <property type="protein sequence ID" value="ENSBIXP00005040699.1"/>
        <property type="gene ID" value="ENSBIXG00005023462.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906514"/>
    <dbReference type="CTD" id="6401"/>
    <dbReference type="GeneTree" id="ENSGT00940000160168"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030863">
        <property type="term" value="C:cortical cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005041143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="398" mass="42397" checksum="122CE198DB2733E1" modified="2019-09-18" version="1">MERKNPSREGSRRLSAKPSKGSEMKKAARQLGLAAAESDKDSGFSDGSSECLSSAEQMESEDMLSALGWSREDKPRPSSKPASGAFPTLSPMVVMKNVLVKQGSSSSQLQSWTVQPSFEVISAQPQLLFLHPPVPSPVSPGHAGEKKSDSRNYLPILNSYTKIAPHPGKRGPAHSAEEREGGMQKKVCTERLGSGLSAREPTKTGAGPPQPPTPAPPSTKLAEDSALQGVPSLVAGGSPQTLQPVSSSHVAKAPSLTFASPASPVCASDSTLHGVESSSPLSPPAASYSSALWAAEHFCRSPDSFPEQRQSKHRRFQNTLVVLHKSGLLEITLKTKELIRQNQATQVELDQLKEQTRLFIEATKSRAPQAWARLQASLSSGSGHTGSDLEAFSEQPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2II76</accession>
    <name>A0A4W2II76_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein 496</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Zinc finger protein with KRAB and SCAN domains 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ZNF496</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-binding transcription factor that can both act as an activator and a repressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via zinc-fingers) with JARID2. Interacts with NSD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027402571.1">
        <property type="nucleotide sequence ID" value="XM_027546770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2II76"/>
    <dbReference type="SMR" id="A0A4W2II76"/>
    <dbReference type="STRING" id="30522.A0A4W2II76"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036377.1">
        <property type="protein sequence ID" value="ENSBIXP00000021612.1"/>
        <property type="gene ID" value="ENSBIXG00000024658.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005039072.1">
        <property type="protein sequence ID" value="ENSBIXP00005043064.1"/>
        <property type="gene ID" value="ENSBIXG00005004553.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113895490"/>
    <dbReference type="CTD" id="84838"/>
    <dbReference type="GeneTree" id="ENSGT00940000161967"/>
    <dbReference type="OMA" id="PWEIQSW"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:005126">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001511">
        <property type="entry name" value="Zinc finger protein 496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001512">
        <property type="entry name" value="Zinc finger protein 496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001758">
        <property type="entry name" value="Zinc finger protein 496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF180">
        <property type="entry name" value="ZINC FINGER PROTEIN 496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="23">
        <location>
            <begin position="42"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="521"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005043064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="587" mass="66361" checksum="9B580359FCD21E6A" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2INI6</accession>
    <name>A0A4W2INI6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein C receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PROCR</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027414517.1">
        <property type="nucleotide sequence ID" value="XM_027558716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2INI6"/>
    <dbReference type="SMR" id="A0A4W2INI6"/>
    <dbReference type="STRING" id="30522.A0A4W2INI6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029994.1">
        <property type="protein sequence ID" value="ENSBIXP00000037799.1"/>
        <property type="gene ID" value="ENSBIXG00000021474.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005054230.1">
        <property type="protein sequence ID" value="ENSBIXP00005045717.1"/>
        <property type="gene ID" value="ENSBIXG00005001603.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113903087"/>
    <dbReference type="CTD" id="10544"/>
    <dbReference type="GeneTree" id="ENSGT00390000001159"/>
    <dbReference type="OMA" id="WGNASLD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050819">
        <property type="term" value="P:negative regulation of coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015669">
        <property type="entry name" value="Endothetial_C_recpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15349">
        <property type="entry name" value="ENDOTHELIAL PROTEIN C RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15349:SF0">
        <property type="entry name" value="ENDOTHELIAL PROTEIN C RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044612158" evidence="3">
        <location>
            <begin position="20"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="MHC class I-like antigen recognition-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005045717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="241" mass="27010" checksum="B5129082F86FEE70" modified="2019-09-18" version="1">MLTTLLPLLPLLLPGWALCSQEASDGPWDLHMTQVSYFRNPSQVWHRGNATLGGVLTHVLEGPGRNVSIQQLQPLQEPDSWALTKIYLNRYLEEFVGLVQVVHQERGVTFPLIIRCFLGCELPPEGSEARVFFEVAVNGSSFVNFQPKTASWVAEPHAPSRVVTYTVDQLNKYNRTRYELREFLQDTCVQYIQKHITTNNLKGSQTGRSYTSLVLGVLVGCFIVTGVAVGIFLCTGGRRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BL25</accession>
    <name>A0A4W2BL25_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BL25"/>
    <dbReference type="STRING" id="30522.A0A4W2BL25"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052895.1">
        <property type="protein sequence ID" value="ENSBIXP00000000547.1"/>
        <property type="gene ID" value="ENSBIXG00000029689.1"/>
    </dbReference>
    <dbReference type="OMA" id="KGEKWID"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07222">
        <property type="entry name" value="Pat_PNPLA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000057">
        <property type="entry name" value="Patatin-like phospholipase domain containing 4, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033902">
        <property type="entry name" value="PNPLA4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF7">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="6"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="253" mass="28192" checksum="8B8E4A74CDE9C5AC" modified="2019-09-18" version="1">MKHINLSFAACGFLGIYHLGAASALYNHGRKLLKDVKACAGASAGSLVASVLLTAPQKIEECNKFTYDFAEEIRRQSFGALTPGYDFMARLRSGVESILPANAHELAHGRLHVSITNTRTRRNCLVSSFPSREDLIKVLLASSFLPIYAGLKPVEYKGQRWVDGGFTNSLPVLPVGRTVTISPFSGRLDVCPQDKGRLHFYVSITNQEVLLSVANLVRLNRALFPPSRRTMELLYRRGFDDASRFLLKENWFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BLG0</accession>
    <name>A0A4W2BLG0_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BLG0"/>
    <dbReference type="STRING" id="30522.A0A4W2BLG0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000336.1">
        <property type="protein sequence ID" value="ENSBIXP00000000703.1"/>
        <property type="gene ID" value="ENSBIXG00000029471.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055328.1">
        <property type="protein sequence ID" value="ENSBIXP00005027293.1"/>
        <property type="gene ID" value="ENSBIXG00005030830.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000184876"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000000703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005027293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="65" mass="7075" checksum="7BC964549F7D5A17" modified="2019-09-18" version="1">MAREKAKPCELDQEKYNADDNVKIICLGDSTGECPCGLLGHSRPRAVPKYACLLLPQGPCLHHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BPM1</accession>
    <name>A0A4W2BPM1_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromodomain Y like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CDYL</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027379998.1">
        <property type="nucleotide sequence ID" value="XM_027524197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BPM1"/>
    <dbReference type="SMR" id="A0A4W2BPM1"/>
    <dbReference type="STRING" id="30522.A0A4W2BPM1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012878.1">
        <property type="protein sequence ID" value="ENSBIXP00000001955.1"/>
        <property type="gene ID" value="ENSBIXG00000000111.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037879.1">
        <property type="protein sequence ID" value="ENSBIXP00005023179.1"/>
        <property type="gene ID" value="ENSBIXG00005004428.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881278"/>
    <dbReference type="CTD" id="9425"/>
    <dbReference type="GeneTree" id="ENSGT00940000155106"/>
    <dbReference type="OMA" id="VPRSPMN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120092">
        <property type="term" value="F:(2E)-butenoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060816">
        <property type="term" value="P:random inactivation of X chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18634">
        <property type="entry name" value="CD_CDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000006">
        <property type="entry name" value="Chromodomain Y-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000012">
        <property type="entry name" value="Chromodomain Y-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000029">
        <property type="entry name" value="chromodomain Y-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF5">
        <property type="entry name" value="CHROMODOMAIN Y-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000001955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="544" mass="60253" checksum="C3101B370A20607C" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQK9</accession>
    <name>A0A4W2BQK9_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UEV and lactate/malate dehyrogenase domains</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQK9"/>
    <dbReference type="STRING" id="30522.A0A4W2BQK9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012193.1">
        <property type="protein sequence ID" value="ENSBIXP00000002291.1"/>
        <property type="gene ID" value="ENSBIXG00000008783.1"/>
    </dbReference>
    <dbReference type="OMA" id="CIMGANG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05293">
        <property type="entry name" value="LDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000040">
        <property type="entry name" value="tumor susceptibility gene 101 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000007">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 variant 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000270">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 variant 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF33">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 VARIANT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UEV" evidence="1">
        <location>
            <begin position="55"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="524" mass="57887" checksum="BB0ECCDC1BFD5D9B" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQL7</accession>
    <name>A0A4W2BQL7_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MID1 interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MID1IP1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPOT14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027389940.1">
        <property type="nucleotide sequence ID" value="XM_027534139.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027389941.1">
        <property type="nucleotide sequence ID" value="XM_027534140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQL7"/>
    <dbReference type="SMR" id="A0A4W2BQL7"/>
    <dbReference type="STRING" id="30522.A0A4W2BQL7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012176.1">
        <property type="protein sequence ID" value="ENSBIXP00000002301.1"/>
        <property type="gene ID" value="ENSBIXG00000008788.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023695.1">
        <property type="protein sequence ID" value="ENSBIXP00005036297.1"/>
        <property type="gene ID" value="ENSBIXG00005017816.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113887183"/>
    <dbReference type="CTD" id="58526"/>
    <dbReference type="GeneTree" id="ENSGT00500000044890"/>
    <dbReference type="OMA" id="EEGNAWK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055103">
        <property type="term" value="F:ligase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045723">
        <property type="term" value="P:positive regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053719">
        <property type="entry name" value="Lipogen_MT_Stabilize_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009786">
        <property type="entry name" value="Spot_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315:SF16">
        <property type="entry name" value="MID1-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315">
        <property type="entry name" value="SPOT14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07084">
        <property type="entry name" value="Spot_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20204" checksum="57CB51B89E310E48" modified="2019-09-18" version="1">MMQICDTYNQKHSLFNAMNRFIGAVNNMDQTVMVPSLLRDVPLAEPELDNDVGVEVGGSGGCMEERTPPAPSPGSANGSFFAPSRDMYSHYVLLKSIRNDIEWGVLHQPPAAGSEEGSAWKSKDILVDLSNLEGADTGEEDLEQQFHYHLRGLHTVLSKLTRKANILTNRYKQEIGFSNWGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BQR6</accession>
    <name>A0A4W2BQR6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPL14</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2BQR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012069.1">
        <property type="protein sequence ID" value="ENSBIXP00000002364.1"/>
        <property type="gene ID" value="ENSBIXG00000008848.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005035601.1">
        <property type="protein sequence ID" value="ENSBIXP00005021639.1"/>
        <property type="gene ID" value="ENSBIXG00005024687.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007888"/>
    <dbReference type="OMA" id="HNYNESH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000022">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="7">
        <location>
            <begin position="47"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="161"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="172"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005021639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="214" mass="23376" checksum="7E0950FFB2304D60" modified="2019-09-18" version="1">AVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINAKWAATRWAKKIEAREKKAKMTDFDRYKVMKARKMRNRLIKIEVKKLQKAALLKASPKKALAAKGAAAVAAAAAAKVPAKKITTEGKKAPAQKAPAQKAAGQKAAPPPKTQKGQKAPSQKAPAPKASGKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BSJ9</accession>
    <name>A0A4W2BSJ9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Negative elongation factor complex member B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BSJ9"/>
    <dbReference type="STRING" id="30522.A0A4W2BSJ9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000010755.1">
        <property type="protein sequence ID" value="ENSBIXP00000003011.1"/>
        <property type="gene ID" value="ENSBIXG00000009429.1"/>
    </dbReference>
    <dbReference type="OMA" id="LLWYIHP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010405">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503:SF3">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR COMPLEX MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06209">
        <property type="entry name" value="COBRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000003011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="69918" checksum="BC340FCC609BC68D" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BYL9</accession>
    <name>A0A4W2BYL9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger protein 330</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nucleolar autoantigen 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ZNF330</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOA36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027423208.1">
        <property type="nucleotide sequence ID" value="XM_027567407.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027423209.1">
        <property type="nucleotide sequence ID" value="XM_027567408.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027423210.1">
        <property type="nucleotide sequence ID" value="XM_027567409.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027423211.1">
        <property type="nucleotide sequence ID" value="XM_027567410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2BYL9"/>
    <dbReference type="STRING" id="30522.A0A4W2BYL9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005734.1">
        <property type="protein sequence ID" value="ENSBIXP00000005450.1"/>
        <property type="gene ID" value="ENSBIXG00000011638.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005011243.1">
        <property type="protein sequence ID" value="ENSBIXP00005029281.1"/>
        <property type="gene ID" value="ENSBIXG00005000423.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113907794"/>
    <dbReference type="CTD" id="27309"/>
    <dbReference type="GeneTree" id="ENSGT00390000017043"/>
    <dbReference type="OMA" id="CQRTRRQ"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010531">
        <property type="entry name" value="NOA36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06524">
        <property type="entry name" value="NOA36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="264"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="320" mass="35982" checksum="27D4B3FC24FC9DD9" modified="2019-09-18" version="1">MPKKKTGARKKAENRREREKQLRASRSTIDLAKHPCNASMECDKCQRRQKNRAFCYFCNSVQKLPICAQCGKTKCMMKSSDCVIKHAGVYSTGLAMVGAICDFCEAWVCHGRKCLSTHACACPLTDAECVECERGVWDHGGRIFSCSFCHNFLCEDDQFEHQASCQVLEAETFKCVSCNRLGQHSCLRCKACFCDDHTRSKVFKQEKGKQPPCPKCGHETQETKDLSMSTRSLKFGRQTGGEEGDGASGYDAYWKNLASDKYAGASYHDEEEDEYEAEDDEEEEDEGGKDSDAESSDLFTNLNLGRTYASGYAHYEEQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2BZC5</accession>
    <name>A0A4W2BZC5_BOBOX</name>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">POU2AF1</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_027563798.1"/>
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000017499"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="entry name" value="PD-C2-AF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="38"/>
        </location>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS52003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004734.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="256" mass="27436" checksum="DC53505BB863465F" modified="2019-09-18" version="1">MLWQKPTAPEQAPAPPRPYQGVRVKEPVKELLRRKRGHASSGASVTPTAVVLPHQPLATYTTVGPSCLDMEVSASTVTEEGALCAGWLSQPAPAALQPLAPWTPYTEYVSHEAVSCPYSADMYVQPVCPSYTVVGPSSVLTYASQPLITNVTTRSAAAPTVGPPLEGPEHQAPLTYFPWPQPLSTLPTSTLQYQPPAPALPGPQFVQLPISIPEPVLPDAEDPRRAIGSLTIDKLLLEEEDSDTYALNHTLSVEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C1K9</accession>
    <name>A0A4W2C1K9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C-terminal-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CTBP2</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C1K9"/>
    <dbReference type="SMR" id="A0A4W2C1K9"/>
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        <property type="protein sequence ID" value="ENSBIXP00000006644.1"/>
        <property type="gene ID" value="ENSBIXG00000012739.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032740.1">
        <property type="protein sequence ID" value="ENSBIXP00005040304.1"/>
        <property type="gene ID" value="ENSBIXG00005022970.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154430"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transcription coregulator binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CtBP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-terminal-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043322">
        <property type="entry name" value="CtBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF3">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="17">
        <location>
            <begin position="44"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="18">
        <location>
            <begin position="141"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="414"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005040304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="445" mass="48947" checksum="413399EE65099A09" modified="2019-09-18" version="1">MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPILKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRVIVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTICHILNLYRRNTWLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAKAFGFSVLFYDPYLQDGTERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNHHLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPESLRNCVNKEFFVTTAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLPAAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2Q6</accession>
    <name>A0A4W2C2Q6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2C2Q6"/>
    <dbReference type="STRING" id="30522.A0A4W2C2Q6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005031.1">
        <property type="protein sequence ID" value="ENSBIXP00000005796.1"/>
        <property type="gene ID" value="ENSBIXG00000011935.1"/>
    </dbReference>
    <dbReference type="OMA" id="PPAFMCP"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003655">
        <property type="entry name" value="aKRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019041">
        <property type="entry name" value="SSXRD_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14112:SF1">
        <property type="entry name" value="KRAB-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14112">
        <property type="entry name" value="SYNOVIAL SARCOMA, X MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09514">
        <property type="entry name" value="SSXRD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50806">
        <property type="entry name" value="KRAB_RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB-related" evidence="2">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="245" mass="28135" checksum="16F20700A0952BBF" modified="2019-09-18" version="1">MNRGSFWENPREDRKKLEKKSKTFKTISQYFSKKEWARLGYSEKITYVNMKRNYETMTRLGLQCTPPAFMCPKNGATKSKRCDSKTKNPREKDEPPQGTSNMQGGKCQKVSASEKKRSKIVPGTSGPEQAQRQLRSQAKASISAQQSKKTAGSKKKKVSAIRLRERKPVAYEEISDPEEESNGNTVDVWANRGQKREPVAQEEISDHEEESNRNTVNVWANRLRKRKPVAYEEISDPEEEEDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C2Z3</accession>
    <name>A0A4W2C2Z3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">BAG family molecular chaperone regulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Bcl-2-associated athanogene 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Bcl-2-binding protein Bis</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BAG3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2C2Z3"/>
    <dbReference type="STRING" id="30522.A0A4W2C2Z3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002424.1">
        <property type="protein sequence ID" value="ENSBIXP00000007141.1"/>
        <property type="gene ID" value="ENSBIXG00000001286.1"/>
    </dbReference>
    <dbReference type="OMA" id="QKGEPSM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010664">
        <property type="term" value="P:negative regulation of striated muscle cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.120:FF:000006">
        <property type="entry name" value="BAG family molecular chaperone regulator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000007">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF12">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="WW" evidence="17">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="18">
        <location>
            <begin position="434"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="122"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="220"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="524"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="541"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="570"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="585" mass="62098" checksum="FD76E6154350A8A8" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C5S6</accession>
    <name>A0A4W2C5S6_BOBOX</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Eutheria</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="Solute_trans_a"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
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            <begin position="120"/>
            <end position="141"/>
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        <location>
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            <end position="268"/>
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        </location>
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            <begin position="364"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Ensembl" id="ENSBIXP00000008138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="405" mass="44664" checksum="E540DB7406F4B9DC" modified="2019-09-18" version="1">MTDTPGLLGTPLAWTPPARPAGPQMERAGNGSQGPGPLFLTSPLARGVSGVFVWAALVLTGHQIYLHLRSYTVPHEQRYIIRLLFIVPVYAFDSWLSLLLLGGHQHYIYFDSVRDCYEAFVIYSFLSLCFQYLGGESAIMAEIRGKPVRTSCFHGTCCLRGMTYSIGFLRFCKQATLQFCIVKPIMALVTIVLQAFGKYHDGDFNVRSGYLYITLVYNASVSLALYALFLFYSATRELLQPFEPVLKFLTIKAVIFLSFWQGLLLAILERCGVIPEVQVIDGSTVGAGTVAAGYQNFIICIEMLFASIALRYAFTCQVYSEKTESSPAPSAPMQSISSGLKETMSPQDIVQDAIHNFSPAYQKYTQQATQEAPRPGAHPSPDGGPGGGRKGRNVEKRMLIPAEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7R5</accession>
    <name>A0A4W2C7R5_BOBOX</name>
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        <recommendedName>
            <fullName evidence="7">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AP1B1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the adaptor complexes large subunit family.</text>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSBIXT00000001443.1">
        <property type="protein sequence ID" value="ENSBIXP00000007620.1"/>
        <property type="gene ID" value="ENSBIXG00000007434.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005014809.1">
        <property type="protein sequence ID" value="ENSBIXP00005001133.1"/>
        <property type="gene ID" value="ENSBIXG00005029397.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000155991"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
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        <property type="entry name" value="B-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
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        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Clathrin_b-adaptin_app_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="9">
        <location>
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            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-adaptin appendage C-terminal subdomain" evidence="10">
        <location>
            <begin position="835"/>
            <end position="948"/>
        </location>
    </feature>
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        <location>
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            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="594"/>
            <end position="616"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="949" mass="104507" checksum="4B45893D57822543" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C7T1</accession>
    <name>A0A4W2C7T1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="gene ID" value="ENSBIXG00000013582.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:sodium ion transport"/>
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        <property type="term" value="P:sodium-dependent phosphate transport"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS_1"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="165"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="200"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <end position="388"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="430"/>
            <end position="450"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="462"/>
            <end position="480"/>
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    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="485"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023201"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00035839"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50850"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000007635.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="496" mass="54225" checksum="55CF58FBCDB43DBE" modified="2019-09-18" version="1">MFNTVEAGTIVGGPVNNDTVNMAQVQTSRKGFCSVRHGLALILHLCNLSISTQQMNMSIALPVMVNNTALPSSPNASTERPPVDSQDNWNETLNESKAMAAVYDWSPEIQGVILSSLNYGSFLAPIPSSYMAEVFGTRYLSGTCMFITSVLALFTPLAADTGVTVLIVLRVIQGIVQVVLLTSQYSIWARWAPPQERSQLITIAVAGSVLGCFIIFLVGGYLCQTIGWPCVFYIFGGIGCVCSFLWFAVIYDDPVSHPFISTKEKEYIVCSLAQEGSPPGWSLPTKAMIKSLPLWSILIFYFSDYWHFFVLTSFLPTFITSVLQANIRDSGILSALPFASACICVVPGGLLADFLLSRKILRLNTIRKLFTAIGVLFPAVLFTSVYWVRSSFSTTIAFLTISSAIKSFAQSGALVNFVDIAPRYSALLRALSQIFSYLAGAISPTVNGFLMSQDPELGWRNVFLLSAAVGILGLIFYLIFGQAEVQDWAKQTCTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C8Y6</accession>
    <name>A0A4W2C8Y6_BOBOX</name>
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        <submittedName>
            <fullName evidence="3">Ataxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ATXN2</name>
    </gene>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSBIXG00000015037.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00005018494.1"/>
        <property type="gene ID" value="ENSBIXG00005022755.1"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="entry name" value="PAM2_motif"/>
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        <property type="entry name" value="ATAXIN 2-RELATED"/>
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        </location>
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        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SMART" id="SM01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="1005" mass="108985" checksum="F75A3424452132D3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C944</accession>
    <name>A0A4W2C944_BOBOX</name>
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        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">28S ribosomal protein S25, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPS25</name>
    </gene>
    <organism evidence="9 10">
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        <name type="common">Hybrid cattle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027379298.1">
        <property type="nucleotide sequence ID" value="XM_027523497.1"/>
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    <dbReference type="SMR" id="A0A4W2C944"/>
    <dbReference type="STRING" id="30522.A0A4W2C944"/>
    <dbReference type="Ensembl" id="ENSBIXT00000011141.1">
        <property type="protein sequence ID" value="ENSBIXP00000002818.1"/>
        <property type="gene ID" value="ENSBIXG00000000270.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005052211.1">
        <property type="protein sequence ID" value="ENSBIXP00005043130.1"/>
        <property type="gene ID" value="ENSBIXG00005026733.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113880863"/>
    <dbReference type="CTD" id="64432"/>
    <dbReference type="GeneTree" id="ENSGT00640000091558"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000103">
        <property type="entry name" value="28S ribosomal protein S25, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="8">
        <location>
            <begin position="36"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000002818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="173" mass="20055" checksum="CBBCE9456F71AE7A" modified="2019-09-18" version="1">MPMKGRFPIRRTLQYLSQGDVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMLFKNMTPTPFLRFYLDSGEQVLVDVETKSNKEIMEHVKKILGKNEETLRRERQEREQLSHPAHFGPRKYCLRECICEVEGQVPCPAVVPLPRELTGKFQAALRAGAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2C9C5</accession>
    <name>A0A4W2C9C5_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Autophagy related 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG2B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATG2 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000043629.1">
        <property type="protein sequence ID" value="ENSBIXP00000008459.1"/>
        <property type="gene ID" value="ENSBIXG00000014310.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026849">
        <property type="entry name" value="ATG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190:SF20">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 2 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13329">
        <property type="entry name" value="ATG2_CAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="867"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1375"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1577"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1769"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="873"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1388"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1781"/>
            <end position="1791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="2084" mass="232765" checksum="01515C0BF7DB9887" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CAT4</accession>
    <name>A0A4W2CAT4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">11-beta-hydroxysteroid dehydrogenase type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Corticosteroid 11-beta-dehydrogenase isozyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">NAD-dependent 11-beta-hydroxysteroid dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HSD11B2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>11beta,17beta-dihydroxyandrost-4-ene-3-one + NAD(+) = 17beta-hydroxyandrost-4-ene-3,11-dione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:34133"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:81481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:69369"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>11beta-hydroxyandrost-4-ene-3,17-dione + NAD(+) = androst-4-ene-3,11,17-trione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69408"/>
            <dbReference type="ChEBI" id="CHEBI:2495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27967"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:69409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an 11beta-hydroxysteroid + NAD(+) = an 11-oxosteroid + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53116"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35346"/>
            <dbReference type="ChEBI" id="CHEBI:47787"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:53117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>corticosterone + NAD(+) = 11-dehydrocorticosterone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16827"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78600"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:42205"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Steroid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with ligand-free cytoplasmic NR3C2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CAT4"/>
    <dbReference type="STRING" id="30522.A0A4W2CAT4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018557.1">
        <property type="protein sequence ID" value="ENSBIXP00000009985.1"/>
        <property type="gene ID" value="ENSBIXG00000015581.1"/>
    </dbReference>
    <dbReference type="OMA" id="GMGLMYF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070523">
        <property type="term" value="F:11-beta-hydroxysteroid dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008211">
        <property type="term" value="P:glucocorticoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09805">
        <property type="entry name" value="type2_17beta_HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000074">
        <property type="entry name" value="Retinol dehydrogenase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF2">
        <property type="entry name" value="11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0753">Steroid metabolism</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="404" mass="43992" checksum="F9B486040D1EE6C6" modified="2019-09-18" version="1">MESWPWPSGGAWLLVAARALLQLLRADLRLGRPLLAALALLAALDWLCQRLLPPLAALAVLAATGWIVLSRLARPQRLPVATRAVLITGCDSGFGNATAKKLDTMGFTVLATVLDLNSPGALELRACCSSRLKLLQMDLTKPADISRVLEFTKVHTASTGLWGLVNNAGQNIFVADAELCPVATFRTCMEVNFFGALEMTKGLLPLLRRSSGRIVTVSSPAGDMPFPCLAAYGTSKAALALLMGNFSCELLPWGVKVSIIQPACFKTESVKDVHQWEERKQQLLATLPQELLQAYGEDYIEHLNGQFLHSLSQALPDLSPVVDAITDALLAAQPRRRYYPGHGLGLIYFIHYYLPEGLRQRFLQSFFISPYVPRALQAGQPGLTSARDIAQDQGPRPDPSPTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBJ3</accession>
    <name>A0A4W2CBJ3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat and fibronectin type-III domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LRFN5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Promotes neurite outgrowth in hippocampal neurons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LRFN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027376638.1">
        <property type="nucleotide sequence ID" value="XM_027520837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBJ3"/>
    <dbReference type="STRING" id="30522.A0A4W2CBJ3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017500.1">
        <property type="protein sequence ID" value="ENSBIXP00000009286.1"/>
        <property type="gene ID" value="ENSBIXG00000015003.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005050053.1">
        <property type="protein sequence ID" value="ENSBIXP00005040436.1"/>
        <property type="gene ID" value="ENSBIXG00005003938.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113879436"/>
    <dbReference type="CTD" id="145581"/>
    <dbReference type="GeneTree" id="ENSGT00940000158296"/>
    <dbReference type="OMA" id="EENAQCF"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905606">
        <property type="term" value="P:regulation of presynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05764">
        <property type="entry name" value="IgI_SALM5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000025">
        <property type="entry name" value="Leucine rich repeat and fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000235">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000091">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000016">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF10">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610419" description="Leucine-rich repeat and fibronectin type-III domain-containing protein 5" evidence="16">
        <location>
            <begin position="18"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="524"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="287"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="385"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="614"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="628"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="717" mass="79079" checksum="0C619FA6D882F6AD" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CBV1</accession>
    <name>A0A4W2CBV1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uroplakin-1a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Uroplakin Ia</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CBV1"/>
    <dbReference type="STRING" id="30522.A0A4W2CBV1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044603.1">
        <property type="protein sequence ID" value="ENSBIXP00000010380.1"/>
        <property type="gene ID" value="ENSBIXG00000015871.1"/>
    </dbReference>
    <dbReference type="OMA" id="REPLWEG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03156">
        <property type="entry name" value="uroplakin_I_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000017">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47110">
        <property type="entry name" value="TESTIS-SPECIFIC EXPRESSED PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47110:SF2">
        <property type="entry name" value="UROPLAKIN-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="414" mass="45457" checksum="615B0B650DCCCD0B" modified="2019-09-18" version="1">MGRGGGGWGVLRRSPFSMPSLFLAHSSPRLLVEHSSQNPVTHTHLPNSPSVPEGSIPSTCLVSSLLPASSSGPGRPGSLPWRGGANQPRRQFWDSGLACGFHLNLSPSQGGSSPAERSRVTPSPLQETGLRRGLGVYREPLWEGLAREATGREGRTMASAAAATTEKGSPVVVGLLVMGNIIILLSGLALFAETVWVTADQYRMYPLMGVSGKDDVFAGAWIAIFCGFSFFVVASFGVGAALCRRRSMILTYLILMLIIYIFECASCITSYTHRDYMVSNPSLITKQMLTFYSADSNQGRELTRLWDRIMIEQECCGTSGPMDWVNFTSAFRATTPEVVFPWPPLCCRRTGNFIPVNEEGCRLGHLDYLFTKGCFEHIGHAIDSYTWGISWFGFAILMWTLPVMLIAMYFYTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CCS1</accession>
    <name>A0A4W2CCS1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Growth/differentiation factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GDF3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027399323.1">
        <property type="nucleotide sequence ID" value="XM_027543522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CCS1"/>
    <dbReference type="SMR" id="A0A4W2CCS1"/>
    <dbReference type="STRING" id="30522.A0A4W2CCS1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017741.1">
        <property type="protein sequence ID" value="ENSBIXP00000009436.1"/>
        <property type="gene ID" value="ENSBIXG00000005958.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005016287.1">
        <property type="protein sequence ID" value="ENSBIXP00005008690.1"/>
        <property type="gene ID" value="ENSBIXG00005013702.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113893622"/>
    <dbReference type="CTD" id="9573"/>
    <dbReference type="GeneTree" id="ENSGT00940000162534"/>
    <dbReference type="OMA" id="DWRTHSR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13764">
        <property type="entry name" value="TGF_beta_GDF1_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000020">
        <property type="entry name" value="growth/differentiation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF38">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00669">
        <property type="entry name" value="INHIBINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044610429" description="Growth/differentiation factor 3" evidence="10">
        <location>
            <begin position="20"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="247"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000009436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="364" mass="40646" checksum="4B9678511EC8B481" modified="2019-09-18" version="1">MLPSLAALALCLLFTLAFGQTLQFHEHVFLRFLGLDKVPSPKKFQPVPSILKRIFQAQEEAASTGISKDLCYVKELGVRGNILRLLPDQGSFLYSESLSHASCLQKLLSFNLSAIGDEEQLTMAQLGLDLGPNTYYNLGPELELSLSLVQTEPHVIDQATPKMGKMFTLQSVPWPQGVLHFNLLDVAKRNNDPHKNLGLFLEIVVKGGRALGENFQLEGTCARLRRSLHTSLLVVTLHPEQCRSPSRKRRAAVSTSKASCKSLCHRHQLFINFRDLGWHKWIIAPKGFMANYCHGECPFSLTISLNSSNYAFMQALMHAVDPEVPQAVCIPTKLSPISMLYQDNDDNVILRHYEDMIVDECGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CDR6</accession>
    <name>A0A4W2CDR6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THAP domain-containing protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">THAP11</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the THAP11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027372027.1">
        <property type="nucleotide sequence ID" value="XM_027516226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CDR6"/>
    <dbReference type="SMR" id="A0A4W2CDR6"/>
    <dbReference type="STRING" id="30522.A0A4W2CDR6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000018736.1">
        <property type="protein sequence ID" value="ENSBIXP00000010085.1"/>
        <property type="gene ID" value="ENSBIXG00000015656.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005032771.1">
        <property type="protein sequence ID" value="ENSBIXP00005040321.1"/>
        <property type="gene ID" value="ENSBIXG00005022909.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876713"/>
    <dbReference type="CTD" id="57215"/>
    <dbReference type="GeneTree" id="ENSGT00390000006585"/>
    <dbReference type="OMA" id="GHRVCSI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903108">
        <property type="term" value="P:regulation of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22291">
        <property type="entry name" value="cc_THAP11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794">
        <property type="entry name" value="THAP DOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000010085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="303" mass="33056" checksum="BB371F5D13359F7E" modified="2019-09-18" version="1">MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQPSPSASTAQTTQLQPNLVSASAAVLLTLQAAVDSSQAPGTVPPVPTTPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELEAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CF16</accession>
    <name>A0A4W2CF16_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">B9 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">B9D2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the B9D family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027371535.1">
        <property type="nucleotide sequence ID" value="XM_027515734.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371536.1">
        <property type="nucleotide sequence ID" value="XM_027515735.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027371537.1">
        <property type="nucleotide sequence ID" value="XM_027515736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CF16"/>
    <dbReference type="STRING" id="30522.A0A4W2CF16"/>
    <dbReference type="Ensembl" id="ENSBIXT00000021051.1">
        <property type="protein sequence ID" value="ENSBIXP00000011600.1"/>
        <property type="gene ID" value="ENSBIXG00000016831.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005023137.1">
        <property type="protein sequence ID" value="ENSBIXP00005036068.1"/>
        <property type="gene ID" value="ENSBIXG00005017466.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113876472"/>
    <dbReference type="CTD" id="80776"/>
    <dbReference type="GeneTree" id="ENSGT00940000161428"/>
    <dbReference type="OMA" id="DVAYWCH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010796">
        <property type="entry name" value="C2_B9-type_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968">
        <property type="entry name" value="B9 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968:SF2">
        <property type="entry name" value="B9 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07162">
        <property type="entry name" value="B9-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51381">
        <property type="entry name" value="C2_B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="175" mass="19358" checksum="23E041118EE6B246" modified="2019-09-18" version="1">MAEVHVIGQIMGATGFSESSLFCKWGVHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHLQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLDCPTWRPLGSWREQLARAFVGGGPQLLHGDAIYSGADRYRLHTTSGGTVHLELSLLLRHFDRYGVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CF57</accession>
    <name>A0A4W2CF57_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SPT3 homolog, SAGA and STAGA complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SUPT3H</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027380650.1">
        <property type="nucleotide sequence ID" value="XM_027524849.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380651.1">
        <property type="nucleotide sequence ID" value="XM_027524850.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380652.1">
        <property type="nucleotide sequence ID" value="XM_027524851.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027380653.1">
        <property type="nucleotide sequence ID" value="XM_027524852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2CF57"/>
    <dbReference type="Ensembl" id="ENSBIXT00000006783.1">
        <property type="protein sequence ID" value="ENSBIXP00000004933.1"/>
        <property type="gene ID" value="ENSBIXG00000011241.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005042449.1">
        <property type="protein sequence ID" value="ENSBIXP00005026300.1"/>
        <property type="gene ID" value="ENSBIXG00005002363.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881773"/>
    <dbReference type="CTD" id="8464"/>
    <dbReference type="GeneTree" id="ENSGT00390000010738"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="270"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000004933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="373" mass="41327" checksum="AD9A225D7F382A2E" modified="2019-09-18" version="1">MNNTAASPMSAATSSSGRSTGKSISFTAELQSMMFSLGDARRPLHETAVLVEDVVHTQLINLLQQAAEVSQLRGARVISAEDLLFLMRKDKKKLRRLLKYMFFRDYKSKIVKGIDEDDLLEDKLSGSNNVNKRQKIAQDFLNSIDQTGELLAMFEDDEIDEVKQERMERAERQTRVMDSAQYAEFCESRHLSFSKKGSKFRDWLDCGSMEIKPNVIAMEILAYLAYETVAQLVDLALLVRQDMVTKAGDPFSHAISATFIQYHNSAEGSCMKPYPESPENTPPPTPTAPSAGSQHSGKTTSGSLGNGSAGQDSAKTKQRKRKKSTAACGVEAHSDAIQPSHIREAIRRYGHKIGPLSPFTSAYRRNGMAFLAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CFP1</accession>
    <name>A0A4W2CFP1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibronectin type-II domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the seminal plasma protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CFP1"/>
    <dbReference type="STRING" id="30522.A0A4W2CFP1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045313.1">
        <property type="protein sequence ID" value="ENSBIXP00000011851.1"/>
        <property type="gene ID" value="ENSBIXG00000017048.1"/>
    </dbReference>
    <dbReference type="OMA" id="KNDYAKC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033700">
        <property type="term" value="P:phospholipid efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902492">
        <property type="term" value="P:positive regulation of sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000003">
        <property type="entry name" value="binder of sperm protein homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000005">
        <property type="entry name" value="Epididymal sperm binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016356">
        <property type="entry name" value="Seminal_plasma_PDC-109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051666">
        <property type="entry name" value="SP_Capacitation_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22918">
        <property type="entry name" value="SEMINAL PLASMA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22918:SF3">
        <property type="entry name" value="SEMINAL PLASMA PROTEIN HSP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002541">
        <property type="entry name" value="Seminal_plasma_PDC-109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0278">Fertilization</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="9">
        <location>
            <begin position="60"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="9">
        <location>
            <begin position="105"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="151" mass="17305" checksum="D8A2CD247C951164" modified="2019-09-18" version="1">MTWSATGEQATMALRLGLFLIWAGVSMFLQLDPVNGDEQLSEDNVILPKEKKDPASGAETKDNKCVFPFIYGNKKYFDCTLHGSLFLWCSLDADYTGRWKYCTKNDYAKCVFPFIYEGKSYDTCIIIGSTFMNYWCSLSSNYDEDGVWKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CGI4</accession>
    <name>A0A4W2CGI4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GST superfamily. Alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CGI4"/>
    <dbReference type="STRING" id="30522.A0A4W2CGI4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005823.1">
        <property type="protein sequence ID" value="ENSBIXP00000005407.1"/>
        <property type="gene ID" value="ENSBIXG00000007746.1"/>
    </dbReference>
    <dbReference type="OMA" id="LDLMMMI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03208">
        <property type="entry name" value="GST_C_Alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000005">
        <property type="entry name" value="Glutathione S-transferase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003080">
        <property type="entry name" value="GST_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF101">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01266">
        <property type="entry name" value="GSTRNSFRASEA"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="85"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000005407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="222" mass="25552" checksum="CFB60B12A99F96E2" modified="2019-09-18" version="1">MAAKPKLWYFHGRGRMESIRWLLAAAGVEFEEEFLETREQYEKLQKDGRLLFGQVPLVEIDGMELTQTRAILSYLAAKYNLHGKDLKETVRIDTYADGTLDLMLMVALAAFKPPEEKEESLALAVKKAKTRYFPVFEKILKDHGEDFLVGNKFSWADIQLLEAILTMEELDASVLSDFPLLKEFKTRISNIPTIKKFLQPGSQRKPPPDSHYVEVVRNILQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CHM6</accession>
    <name>A0A4W2CHM6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein ZFAT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Zinc finger protein 406</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZFAT</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May be involved in transcriptional regulation. Overexpression causes down-regulation of a number of genes involved in the immune response. Some genes are also up-regulated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSBIXT00000020516.1">
        <property type="protein sequence ID" value="ENSBIXP00000011244.1"/>
        <property type="gene ID" value="ENSBIXG00000006101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000255">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000297">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000306">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000327">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000351">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000401">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000442">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000687">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001388">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001389">
        <property type="entry name" value="Zinc finger and AT-hook domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF67">
        <property type="entry name" value="FI01116P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="402"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="506"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="788"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="876"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="955"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="983"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1012"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1040"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="100"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="582"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="687"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="649"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000011244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1289" mass="142279" checksum="8E0CEDB68643CB90" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CJS5</accession>
    <name>A0A4W2CJS5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CJS5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003495.1">
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        <property type="gene ID" value="ENSBIXG00000012661.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021429025" description="RNA-directed DNA polymerase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
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            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000006562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="418" mass="47994" checksum="774F6A5A239E926E" modified="2019-09-18" version="1">MLIILLVMLKILQATLQQYVNHELPDVQAGFRKGRGTRDQIANIRWIIEKAREFQKNIYFCFIDYAKAFDCADHKKLWKILQEMGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLLNLYAEYIMRNTGLEEAQAGIKITGRNINNLRYADDTTLMAESEEELKSLLMKVKEESEEVGLKLNIQKTKIMASSPITSWEIDGETVETVSDYFFGSKITADGDCSHEIKRHLLLGRKVMTNLESIFKSRDITLPTKVRLVKAMVFPVVMYGCESWTVKKAECRRIDAFELWCWRRLLRVPWTARRSNQSILKEISPGCSLEGLMLKLKLQYSGHLMQRVDSLEKTLMLGGIEGTRKRGRQRMRWLDGITDPTDVSLSELRELVMDREAWRAVIHGVANSRTQLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CPK8</accession>
    <name>A0A4W2CPK8_BOBOX</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
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                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="290" mass="32027" checksum="7FB843A57BE02F6B" modified="2019-09-18" version="1">MICLSSVSMFILLGFSEYPRLQAPLFLVFLTIYTVTLVGNLGIIVVIRTTIYPFWIFALPALFIPKLLGILVVEDRFFFGCAFVITEMSMLAAMAYDLFVAVCNPLFYTIAMSPKLCSLLVTGTYTWGGMCSLTITCSLLELSFCGSNVIHQFGCEYSAIISASCSDTHFSQLMCFIISTLNEVCSLLIILTSYAFIVVTIIKMPSAGGLRKAFSTCASHLTAITIFHGTVLFLYCVPHSKSSWLLVKVVTVFFTVMIPMLNPLICSLRNKDVKKTVRSLTTMKLLSPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQ84</accession>
    <name>A0A4W2CQ84_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer with RPLP2 at the lateral ribosomal stalk of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQ84"/>
    <dbReference type="SMR" id="A0A4W2CQ84"/>
    <dbReference type="STRING" id="30522.A0A4W2CQ84"/>
    <dbReference type="Ensembl" id="ENSBIXT00000024703.1">
        <property type="protein sequence ID" value="ENSBIXP00000014010.1"/>
        <property type="gene ID" value="ENSBIXG00000003299.1"/>
    </dbReference>
    <dbReference type="OMA" id="YSAHDHE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF38">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="114" mass="11574" checksum="82A83231C420F0D0" modified="2019-09-18" version="1">MASVSELACIYSALILHDDEVTVMEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPTPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CQT2</accession>
    <name>A0A4W2CQT2_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BPTF-associated chromatin complex component 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">BPTF-associated protein of 18 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Chromatin complexes subunit BAP18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BACC1</name>
        <name evidence="12" type="synonym">C17orf49</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of chromatin complexes such as the MLL1/MLL and NURF complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2CQT2"/>
    <dbReference type="SMR" id="A0A4W2CQT2"/>
    <dbReference type="STRING" id="30522.A0A4W2CQT2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000043740.1">
        <property type="protein sequence ID" value="ENSBIXP00000008679.1"/>
        <property type="gene ID" value="ENSBIXG00000014465.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005037999.1">
        <property type="protein sequence ID" value="ENSBIXP00005042583.1"/>
        <property type="gene ID" value="ENSBIXG00005026070.1"/>
    </dbReference>
    <dbReference type="CTD" id="124944"/>
    <dbReference type="GeneTree" id="ENSGT00390000014721"/>
    <dbReference type="OMA" id="GKWADED"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016589">
        <property type="term" value="C:NURF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1880:FF:000003">
        <property type="entry name" value="chromatin complexes subunit BAP18 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397:SF2">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000008679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005042583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="172" mass="17960" checksum="FF0881CCADAAAE5C" modified="2019-09-18" version="1">MTSASTKVGEIFSAAGAAFTKLGELTMQLHPVADSSPAGAKWTETEIEMLRAAVKRFGDDLNHISCVIKERTVAQIKATVKRKVYEDSGIPLPADSPKKGPKKVASGVLSPPPTAPPPSSSSVPEAGGPPIKKQKADVTLSALNDSDANSDLVDIEGLGETPPTKKLNFDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CRB0</accession>
    <name>A0A4W2CRB0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="30522.A0A4W2CRB0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047169.1">
        <property type="protein sequence ID" value="ENSBIXP00000015734.1"/>
        <property type="gene ID" value="ENSBIXG00000003667.1"/>
    </dbReference>
    <dbReference type="OMA" id="SEQNQVG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000015734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1293" mass="147903" checksum="C4A8CA4E36882DE2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:adenosine to inosine editing"/>
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        <property type="entry name" value="DOUBLE-STRANDED RNA-SPECIFIC EDITASE B2"/>
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        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    </dbReference>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000014696.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CUA3</accession>
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        <recommendedName>
            <fullName evidence="8">GTP:AMP phosphotransferase AK3, mitochondrial</fullName>
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        <alternativeName>
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        <alternativeName>
            <fullName evidence="8">Adenylate kinase 3 alpha-like 1</fullName>
        </alternativeName>
    </protein>
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    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
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        <reaction evidence="7">
            <text>GTP + AMP = GDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:29863"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_027549347.1"/>
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        <property type="protein sequence ID" value="ENSBIXP00000016932.1"/>
        <property type="gene ID" value="ENSBIXG00000000297.1"/>
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    <dbReference type="Ensembl" id="ENSBIXT00005026381.1">
        <property type="protein sequence ID" value="ENSBIXP00005015495.1"/>
        <property type="gene ID" value="ENSBIXG00005019326.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113897065"/>
    <dbReference type="CTD" id="50808"/>
    <dbReference type="GeneTree" id="ENSGT00940000155120"/>
    <dbReference type="OMA" id="TIAHFST"/>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03169">
        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028586">
        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="10">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="8">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="8">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="227" mass="25671" checksum="2C29AEF195FBFBFA" modified="2019-09-18" version="1">MGASARLLRAAIMGAPGSGKGTVSSRITKHFELKHLSSGDLLRDNMLRGTEIGVLAKTFIDQGKLIPDDVMTRLVLHELKNLTQYNWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPGSGRVYNIEFNPPKTMGIDDLTGEPLVQREDDRPETVVKRLKAYEAQTEPVLEYYRKKGVLETFSGTETNKIWPHVYAFLQTKLPQRSQETSVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CXT3</accession>
    <name>A0A4W2CXT3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystic fibrosis transmembrane conductance regulator</fullName>
            <ecNumber evidence="6">5.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">ATP-binding cassette sub-family C member 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Channel conductance-controlling ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">cAMP-dependent chloride channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>ATP + H2O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel.</text>
            <dbReference type="EC" id="5.6.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>hydrogencarbonate(in) = hydrogencarbonate(out)</text>
            <dbReference type="Rhea" id="RHEA:28695"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="32">Monomer; does not require oligomerization for channel activity. May form oligomers in the membrane. Interacts with SLC26A3, SLC26A6 and NHERF1. Interacts with SHANK2. Interacts with MYO6. Interacts (via C-terminus) with GOPC (via PDZ domain); this promotes CFTR internalization and thereby decreases channel activity. Interacts with SLC4A7 through NHERF1. Found in a complex with MYO5B and RAB11A. Interacts with ANO1. Interacts with SLC26A8. Interacts with AHCYL1; the interaction increases CFTR activity. Interacts with CSE1L. The core-glycosylated form interacts with GORASP2 (via PDZ GRASP-type 1 domain) in respone to ER stress. Interacts with MARCHF2; the interaction leads to CFTR ubiqtuitination and degradation. Interacts with ADGRG2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.6" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2CXT3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000028687.1">
        <property type="protein sequence ID" value="ENSBIXP00000016658.1"/>
        <property type="gene ID" value="ENSBIXG00000020774.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005260">
        <property type="term" value="F:intracellularly ATP-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18593">
        <property type="entry name" value="ABC_6TM_MRP4_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000027">
        <property type="entry name" value="ATP-binding cassette subfamily C member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000482">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030240">
        <property type="entry name" value="ABCC4_TMD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009147">
        <property type="entry name" value="CFTR/ABCC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF357">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01851">
        <property type="entry name" value="CYSFIBREGLTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0868">Chloride</keyword>
    <keyword evidence="19" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="22" id="KW-0413">Isomerase</keyword>
    <keyword evidence="23" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 33" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00093570"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
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        <source>
            <dbReference type="PROSITE" id="PS50929"/>
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        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016658.1"/>
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    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    <sequence length="882" mass="99404" checksum="BB2392D891F852C1" modified="2019-09-18" version="1"> </sequence>
</entry>
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                <person name="Tearle R."/>
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                <person name="Rosen B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
    <sequence length="418" mass="48806" checksum="D8AC802B7E9FA26B" modified="2019-09-18" version="1">MICFSFSIFITFFLSCIRHNRVKYLCYSQFADSETSLKRQHRACSCRYAQTSQQQPLPLDRNRQDPMTSLYFQHIEMEPNNSFRVDSEFRYTLFPIFYSIVFVLGVIANSYVLWVFARLYPSKKFNEIKIFMVNLTMADLLFLVTLPLWIVYYYNQGDWILPKFLCNLAGCFFFINTYCSVAFLAVITYNRFQAVTRPIKTAQATTRKRGILLSLIIWVSIVGAASYFFVLDSTNREPNKTGSANITRCFEHYEKGSIPVLTIHIFLVFSFFLVFLIILFCNLVIIRTLLTQQVQIQRNAEVKRRALWMVCTVLAVFIICFVPHHLVQLPWTLAELGFQDTDFHQAINDAHQVTLCLLSTNCVLDPIIYCFLTKKFRKHLTEKLYSMRESRKCSRATSETGTEVVMQLKDVPVKSLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2CYY3</accession>
    <name>A0A4W2CYY3_BOBOX</name>
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        <recommendedName>
            <fullName evidence="17">ADAM metallopeptidase domain 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC113897899</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="STRING" id="30522.A0A4W2CYY3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000048582.1">
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        <property type="gene ID" value="ENSBIXG00000006913.1"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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</entry>
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        </lineage>
    </organism>
    <reference evidence="22 24 25" key="1">
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                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
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                <person name="Phillippy A.M."/>
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                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation. Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">The phosphorylated form (by SRPK2) is a component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4. Interacts with ERCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D116"/>
    <dbReference type="STRING" id="30522.A0A4W2D116"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030586.1">
        <property type="protein sequence ID" value="ENSBIXP00000017865.1"/>
        <property type="gene ID" value="ENSBIXG00000004177.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005028481.1">
        <property type="protein sequence ID" value="ENSBIXP00005016924.1"/>
        <property type="gene ID" value="ENSBIXG00005020544.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155606"/>
    <dbReference type="OMA" id="ARDIKHM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062176">
        <property type="term" value="P:R-loop processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000322">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000520">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057479">
        <property type="entry name" value="PRP28/DDX23-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25430">
        <property type="entry name" value="DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="511"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="740"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="16"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="94"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="155"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="236"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00005016924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="909" mass="105068" checksum="2FA3E74EFC31F2E6" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D1G8</accession>
    <name>A0A4W2D1G8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">renin</fullName>
            <ecNumber evidence="4">3.4.23.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Angiotensinogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.</text>
            <dbReference type="EC" id="3.4.23.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.15" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2D1G8"/>
    <dbReference type="STRING" id="30522.A0A4W2D1G8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045609.1">
        <property type="protein sequence ID" value="ENSBIXP00000012487.1"/>
        <property type="gene ID" value="ENSBIXG00000000742.1"/>
    </dbReference>
    <dbReference type="OMA" id="DMQYYGE"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002003">
        <property type="term" value="P:angiotensin maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05487">
        <property type="entry name" value="renin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000032">
        <property type="entry name" value="renin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000037">
        <property type="entry name" value="Renin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034135">
        <property type="entry name" value="Renin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF24">
        <property type="entry name" value="RENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021283205" description="renin" evidence="16">
        <location>
            <begin position="24"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="17">
        <location>
            <begin position="86"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="117"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="283"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="326"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000012487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="407" mass="44806" checksum="6F8AF95232DF41E9" modified="2019-09-18" version="1">VDQCSKMPLWGLLLVLWGSCTFSLPADTAAFRRIFLKKMPSVRESLKERGVDMARLGAEWSQLTKTLSFGNRTSPVVLTNYLDTQYYGEIGIGTPPQTFKVVFDTGSANLWVPSTKCSPLYTACEIHSLYDSLESSSYVENGTEFTIHYGSGKVKGFLSQDLVTVGGITVTQTFGEVTELPLLPFMLAKFDGVLGMGFPAQAVGGITPVFDHILAQRVLTDDVFSVYYSRDSKNSHLLGGEIVLGGSDPQYYQENFHYVSISKPGSWQIRMKGVSVRSTTLLCEEGCMVIVDTGASYISGPTSSLRLLMEALGAKELSIDKYVVNCNQMPTLPDISFHLGGKAYTLTSADYVLQDPYNNDDLCTLALHGMDIPPPTGPVWVLGATFIRKFYTEFDRRNNRIGFALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4F0</accession>
    <name>A0A4W2D4F0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4F0"/>
    <dbReference type="STRING" id="30522.A0A4W2D4F0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032514.1">
        <property type="protein sequence ID" value="ENSBIXP00000019077.1"/>
        <property type="gene ID" value="ENSBIXG00000006514.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF36">
        <property type="entry name" value="RIBOSOMAL PROTEIN EL39-LIKE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="12162" checksum="5BA13BA4BEBC22D9" modified="2019-09-18" version="1">GIKYCWVFTSWGQSRTLLRTPTFQAGHSGLRKGRRALFSSPPSYVRLSSLLTMSSHKTFRIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRGHWRRTKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D4V3</accession>
    <name>A0A4W2D4V3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RNF168</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RING finger protein 168</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RNF168</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">RNF168</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. Interacts with UBE2N/UBC13.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <text evidence="11">Localizes to double-strand breaks (DSBs) sites of DNA damage.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The MIU motif (motif interacting with ubiquitin) mediates the interaction with both 'Lys-48'- and 'Lys-63'-linked ubiquitin chains. The UMI motif mediates interaction with ubiquitin with a preference for 'Lys-63'-linked ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs).</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs).</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Ubiquitinated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNF168 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">According to a well-established model, RNF168 cannot initiate H2A 'Lys-63'-linked ubiquitination and is recruited following RNF8-dependent histone ubiquitination to amplify H2A 'Lys-63'-linked ubiquitination. However, other data suggest that RNF168 is the priming ubiquitin ligase by mediating monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub respectively). These data suggest that RNF168 might be recruited to DSBs sites in a RNF8-dependent manner by binding to non-histone proteins ubiquitinated via 'Lys-63'-linked and initiates monoubiquitination of H2A, which is then amplified by RNF8. Additional evidences are however required to confirm these data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027393095.1">
        <property type="nucleotide sequence ID" value="XM_027537294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2D4V3"/>
    <dbReference type="SMR" id="A0A4W2D4V3"/>
    <dbReference type="STRING" id="30522.A0A4W2D4V3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000032825.1">
        <property type="protein sequence ID" value="ENSBIXP00000019258.1"/>
        <property type="gene ID" value="ENSBIXG00000022914.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043288.1">
        <property type="protein sequence ID" value="ENSBIXP00005026832.1"/>
        <property type="gene ID" value="ENSBIXG00005002067.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889897"/>
    <dbReference type="CTD" id="165918"/>
    <dbReference type="GeneTree" id="ENSGT00940000153680"/>
    <dbReference type="OMA" id="SWARYNT"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21952">
        <property type="entry name" value="MIU2_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16550">
        <property type="entry name" value="RING-HC_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000466">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03066">
        <property type="entry name" value="RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034725">
        <property type="entry name" value="RNF168"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051657">
        <property type="entry name" value="RNF168/RNF169_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="196"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="371"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="456"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="540"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LR motif 1" evidence="11">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="UMI motif" evidence="11">
        <location>
            <begin position="143"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="MIU motif 1" evidence="11">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="MIU motif 2" evidence="11">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LR motif 2" evidence="11">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="494"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03066"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000019258.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="573" mass="65846" checksum="194EF9047A8D465D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2D676</accession>
    <name>A0A4W2D676_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Polydom</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SVEP1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Interacts (via Sushi domain 21) with ITGA9:ITGB1; thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction. Interacts (via Sushi domain 21) with ITGA4:ITGB1; thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction. Interacts with ANGPT1 and ANGPT2. Interacts with PEAR1 (via extracellular domain). Interacts with HSPG2, TLN1, FN1, COPA, CCT2, IQGAP1, LAMC1 and NID1. Interacts (via C-terminus) with TIE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:integrin binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:cell adhesion"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
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    </dbReference>
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        <property type="entry name" value="Protein crumbs homolog 2"/>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
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        <property type="entry name" value="Versican core protein"/>
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    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Stereocilium</location>
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        <property type="gene ID" value="ENSBIXG00000025056.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:actin filament bundle assembly"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:sensory perception of sound"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        </source>
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    <sequence length="989" mass="106313" checksum="C444DCB8A1E6C8B3" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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</entry>
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    <accession>A0A4W2DDK8</accession>
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        <submittedName>
            <fullName evidence="5">Striatin interacting protein 2</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">STRIP2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STRIP family.</text>
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        <property type="protein sequence ID" value="ENSBIXP00000016858.1"/>
        <property type="gene ID" value="ENSBIXG00000020944.1"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF6">
        <property type="entry name" value="STRIATIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="3">
        <location>
            <begin position="48"/>
            <end position="354"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="812"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000016858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="834" mass="95237" checksum="D6040150BD6AEBB4" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DES1</accession>
    <name>A0A4W2DES1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Deubiquitinase MYSM1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Myb-like, SWIRM and MPN domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">MYSM1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67A family. MYSM1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027392907.1">
        <property type="nucleotide sequence ID" value="XM_027537106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DES1"/>
    <dbReference type="SMR" id="A0A4W2DES1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051683.1">
        <property type="protein sequence ID" value="ENSBIXP00000024613.1"/>
        <property type="gene ID" value="ENSBIXG00000026728.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005025919.1">
        <property type="protein sequence ID" value="ENSBIXP00005015190.1"/>
        <property type="gene ID" value="ENSBIXG00005019087.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113889816"/>
    <dbReference type="CTD" id="114803"/>
    <dbReference type="GeneTree" id="ENSGT00940000157721"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08067">
        <property type="entry name" value="MPN_2A_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000193">
        <property type="entry name" value="histone H2A deubiquitinase MYSM1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000151">
        <property type="entry name" value="histone H2A deubiquitinase MYSM1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000018">
        <property type="entry name" value="histone H2A deubiquitinase MYSM1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Myb-like" evidence="18">
        <location>
            <begin position="111"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="21">
        <location>
            <begin position="114"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="22">
        <location>
            <begin position="118"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="20">
        <location>
            <begin position="372"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="MPN" evidence="19">
        <location>
            <begin position="577"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="168"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000024613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="831" mass="95364" checksum="C86873873C632E5D" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DG97</accession>
    <name>A0A4W2DG97_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZDHHC23</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="12"/>
    <dbReference type="RefSeq" id="XP_027379638.1">
        <property type="nucleotide sequence ID" value="XM_027523837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027379726.1">
        <property type="nucleotide sequence ID" value="XM_027523925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DG97"/>
    <dbReference type="STRING" id="30522.A0A4W2DG97"/>
    <dbReference type="Ensembl" id="ENSBIXT00000030579.1">
        <property type="protein sequence ID" value="ENSBIXP00000017849.1"/>
        <property type="gene ID" value="ENSBIXG00000021776.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044265.1">
        <property type="protein sequence ID" value="ENSBIXP00005008621.1"/>
        <property type="gene ID" value="ENSBIXG00005013663.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113881016"/>
    <dbReference type="CTD" id="254887"/>
    <dbReference type="GeneTree" id="ENSGT00940000156558"/>
    <dbReference type="OMA" id="GNWSEFM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF475">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="14">
        <location>
            <begin position="250"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="210"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000017849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="429" mass="48568" checksum="5AD04D2D7EA31F7E" modified="2019-09-18" version="1">MKPVKKTKAEEPELEPLCCCEYIDRNGEKNHVAACLCDCQDLDEGCDRWITCKSVQPETCEKIMDTISDRLRIPWLRGAKKVNISILPPLFLLPVFLRVASWHFLLGVVVLTSLPVLALWYYYLTHRRKEQTLFFLSLGLFSLGYMYYVFLQEVVPKGRVGPTRLALLTCGLLLILFALYRAKKNPGYLRNPAHNDRALSGSQVECLNRKGPEKPKGFPSADLSGGLNNRAPKDETKDSPRTSAGSPAKGKDDWCAKCQLVRPARAWHCRICGICVRRMDHHCVWINSCVGESNHQAFILALLVFLLTSVYGITLTLDTICTDRSVFTALFYCPGVYANYSSALCFTCVWYSVIITGSMAYIFLIQLINISYNVTEREVQQALRQKTGRRLLCGLIVDTGQYNRGFLRNWHQFSTLGAHPFHHPAEDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DJ86</accession>
    <name>A0A4W2DJ86_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TSPY</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DJ86"/>
    <dbReference type="Ensembl" id="ENSBIXT00000053548.1">
        <property type="protein sequence ID" value="ENSBIXP00000000224.1"/>
        <property type="gene ID" value="ENSBIXG00000030087.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000053551.1">
        <property type="protein sequence ID" value="ENSBIXP00000000221.1"/>
        <property type="gene ID" value="ENSBIXG00000030088.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000053560.1">
        <property type="protein sequence ID" value="ENSBIXP00000026403.1"/>
        <property type="gene ID" value="ENSBIXG00000030094.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00000053565.1">
        <property type="protein sequence ID" value="ENSBIXP00000000217.1"/>
        <property type="gene ID" value="ENSBIXG00000030097.1"/>
    </dbReference>
    <dbReference type="OMA" id="EPRMETD"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000026403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36483" checksum="20E8FB83BFC9905F" modified="2019-09-18" version="1">MSRPFASAPARGHRQGQEERERRSEEGGSVPGPRTFQVVSPVVTPGQEATLFRVEAVEEGEALVDGDVAGIGREFQLLAEDIVEEVEVVADEEQEQRPSQELEEKTVEEQGQERPGGPCERQELDALQALAALQVELSSEREQNRRAYVQFMRKNHQRRKRHLARRSAIIQGIPGFWAKAIMSHPQVSVLISDQDQDFLGYMMDLKVQVRSHPPSRCKLIFSFRDNPYFLNSVIIKEYYLDITGYRARRSTPVHWFWDFERGAPSRRLDTRSLNFLNWLSGHNGPESNRIAEIISEDVWDDPLKYYLREEGSSMRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DR72</accession>
    <name>A0A4W2DR72_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027396875.1">
        <property type="nucleotide sequence ID" value="XM_027541074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2DR72"/>
    <dbReference type="STRING" id="30522.A0A4W2DR72"/>
    <dbReference type="Ensembl" id="ENSBIXT00000036205.1">
        <property type="protein sequence ID" value="ENSBIXP00000021494.1"/>
        <property type="gene ID" value="ENSBIXG00000024565.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005020716.1">
        <property type="protein sequence ID" value="ENSBIXP00005011694.1"/>
        <property type="gene ID" value="ENSBIXG00005016059.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113892324"/>
    <dbReference type="CTD" id="389177"/>
    <dbReference type="GeneTree" id="ENSGT00390000016029"/>
    <dbReference type="OMA" id="HYEWYHL"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000021494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="187" mass="20752" checksum="8CCEF7BAF62CD932" modified="2019-09-18" version="1">MQHLYQSAGWVLITLGALSVFSGVIAFFPVFSCKFWFTGWSVWIACPIWNGVLAVTTGILLLLAHKEWTQRYLWEASFTFVILSMMGCPLHFTVALVSALLGPYCFYSLSGIAGINYLGYAVAFPFPYAKFPSVCVDPPHYEEYHLTLQAFDLCLSSAMLCVSLTAFITLSARLIQNGHVNGQKNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DTA5</accession>
    <name>A0A4W2DTA5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Interleukin-17A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms complexes with IL17RA and IL17RC receptors with 2:1 binding stoichiometry: two receptor chains for one interleukin molecule. IL17A homodimer preferentially drives the formation of IL17RA-IL17RC heterodimeric receptor complex. IL17A homodimer adopts an asymmetrical ternary structure with one IL17RA molecule, allowing for high affinity interactions of one IL17A monomer with one IL17RA molecule (via D1 and D2 domains), while disfavoring binding of a second IL17RA molecule on the other IL17A monomer. Heterodimer with IL17F. IL17A-IL17F forms complexes with IL17RA-IL17RC, but with lower affinity when compared to IL17A homodimer. IL17RA and IL17RC chains cannot distinguish between IL17A and IL17F molecules, potentially enabling the formation of topologically distinct complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DTA5"/>
    <dbReference type="SMR" id="A0A4W2DTA5"/>
    <dbReference type="STRING" id="30522.A0A4W2DTA5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005970.1">
        <property type="protein sequence ID" value="ENSBIXP00000029820.1"/>
        <property type="gene ID" value="ENSBIXG00000011544.1"/>
    </dbReference>
    <dbReference type="OMA" id="SPWDYNV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000038">
        <property type="entry name" value="Interleukin-17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020440">
        <property type="entry name" value="IL-17_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01932">
        <property type="entry name" value="INTRLEUKIN17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021303443" description="Interleukin-17A" evidence="10">
        <location>
            <begin position="31"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="170" mass="19188" checksum="42CF172E1C2E5F07" modified="2019-09-18" version="1">MTMTVLCDTAVVKFLLLLTLWLTLLGEAAAQKPPRSGDSGLCPTLEDHIVRVDIRIRRRNQGVFFSRNLQNRSISPWDYNITRDANRFPSEIAEAQCRHMGCINAEGQEDRTLNSVPIQQEFLVLRRKQNGCPGLFQLEKVLVTVGCTCVTPMVRHLHDPMADQVADQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DU77</accession>
    <name>A0A4W2DU77_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Notchless protein homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NLE1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DU77"/>
    <dbReference type="STRING" id="30522.A0A4W2DU77"/>
    <dbReference type="Ensembl" id="ENSBIXT00000012420.1">
        <property type="protein sequence ID" value="ENSBIXP00000027749.1"/>
        <property type="gene ID" value="ENSBIXG00000000170.1"/>
    </dbReference>
    <dbReference type="OMA" id="AWEPYHR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000129">
        <property type="entry name" value="Notchless homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="9">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="155"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="198"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="245"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="413"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000027749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="488" mass="53385" checksum="493E6B3EF70FAD79" modified="2019-09-18" version="1">MAAAAAAVGDEAATRDVQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEDPLPLAFYVHDAEIVSSLGRTLESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCQGHRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGKQVGRALTGHSKWITALSWEPLHANPECRYVASSSKDGSVRVWDTTAGRCERTLTGHAQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNAQDLRGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLARMTGHQALINQVVFSPDSRVIASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVWDVKAQKLSTDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DWH6</accession>
    <name>A0A4W2DWH6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cell membrane</location>
            <topology evidence="3 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2DWH6"/>
    <dbReference type="STRING" id="30522.A0A4W2DWH6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000002274.1">
        <property type="protein sequence ID" value="ENSBIXP00000031050.1"/>
        <property type="gene ID" value="ENSBIXG00000013195.1"/>
    </dbReference>
    <dbReference type="OMA" id="CRCAPLV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000082">
        <property type="entry name" value="Olfactory receptor 1A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="38"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="301" mass="33051" checksum="6169A8FD835836A4" modified="2019-09-18" version="1">MNCSKNPDFVLSGLSNDPDKPQLLFGLFLALYLLSLLGNSLLLLAIGADIHLHTSMYFFLSQLSLVDLCFTTTTAPKMLETLWTSNGLISFSECLAQLYFFMVFADMDNLLLTAMAIDRYTAIRHLLHYAPLMTPCRCAPLVGGSWGVAHSISLAHALLLSQLSFDSNQEIPHFFCDFRPLLRFSCSDTHLNEDLMMVLTGLLGISPLLCIISSYAHIFLAVARVPSAQGKKKALATCSSHLSMVILFYSSVFATYLKSPSASHASGELGAAVMYDPVTPTLNPFIYSPRNEDCPNSVKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2DZI7</accession>
    <name>A0A4W2DZI7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Family with sequence similarity 169 member B</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2DZI7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000017013.1">
        <property type="protein sequence ID" value="ENSBIXP00000032232.1"/>
        <property type="gene ID" value="ENSBIXG00000014753.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029625">
        <property type="entry name" value="FAM169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22442">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22442:SF4">
        <property type="entry name" value="PROTEIN FAM169BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000032232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="42358" checksum="D15B5799C4A48B11" modified="2019-09-18" version="1">MLTTLGGRAQVQGQGGPELPAAISGIYPVDILEDDPAGHQEATLAYYAMLEEGAGPSPEVFSLPTGEQVKLEASSVCFCTVHRDEPQHKILVLVNPQDTKTVVAVYVKQSWWATEDVLRTSNPSREGLMKVQSFGERIVLFILNVVIFGRLERNLDDDDMFFLPHSVKEEAKILWRDGAAVGFYTTKRKGSLCGDSTGTCYLLPVFDTVFVRRRHRRRGLGLTMLQDFCQTFREDEALGVSWPISPAMYQVCRKFLAAHPAERGRLWEVEPPGAWGQRDNIWLKVQLQQSRLHPDSHPGHSKEAVATRGQTSQDDGPGLSHNGEDHKERISRDREQTDGDHAVKAAAGWLARACWAGQEGRTVAGAGLPGGPACSPAPDPRRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E5Q1</accession>
    <name>A0A4W2E5Q1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kidney associated antigen 1</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="30522.A0A4W2E5Q1"/>
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        <property type="gene ID" value="ENSBIXG00000013683.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSBIXP00005004624.1"/>
        <property type="gene ID" value="ENSBIXG00005010498.1"/>
    </dbReference>
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    <dbReference type="OMA" id="APCEEGV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="entry name" value="KAAG1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15354">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="9661" checksum="CFFFED5140162F55" modified="2019-09-18" version="1">VEDDTAAREEGVPVAVHQHALHDRLRQVPGPGTGATHLPLGTPARLASPGGGTAAAPAASQHTRGAGSRPETNAKQTSPEKPSIFESLPCVCVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E621</accession>
    <name>A0A4W2E621_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Adaptor related protein complex 3 subunit sigma 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AP3S1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E621"/>
    <dbReference type="STRING" id="30522.A0A4W2E621"/>
    <dbReference type="Ensembl" id="ENSBIXT00000000721.1">
        <property type="protein sequence ID" value="ENSBIXP00000031555.1"/>
        <property type="gene ID" value="ENSBIXG00000013836.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005027072.1">
        <property type="protein sequence ID" value="ENSBIXP00005015972.1"/>
        <property type="gene ID" value="ENSBIXG00005019729.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193421"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14834">
        <property type="entry name" value="AP3_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000001">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027155">
        <property type="entry name" value="APS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="62"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="247" mass="27634" checksum="BB0AF5E237689995" modified="2019-09-18" version="1">MTSSLSCSPQEEAFLLESHLYFAWLNTASRLCRRGSDAPLCSIIFFSTWFGEAGELAQQMKSEDTQQQIIRETFHLVSKRDENVCNFLEGGLLIGGSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVHNILAEMVMGGMVLETNMNEIVTQIDAQNKLEKSELIVYTGLFCISQDFSQAGLAGAPARAVSAVKNMNLPEIPRNINIGDISIKVPNLPSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E7B7</accession>
    <name>A0A4W2E7B7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC113904855</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Bos indicus x Bos taurus</name>
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        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A4W2E7B7"/>
    <dbReference type="STRING" id="30522.A0A4W2E7B7"/>
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        <property type="protein sequence ID" value="ENSBIXP00000034895.1"/>
        <property type="gene ID" value="ENSBIXG00000018022.1"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR2D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    </feature>
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            <end position="290"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="314" mass="35360" checksum="E822B8FC447F49F1" modified="2019-09-18" version="1">MGEENHTFVAEFIFLGLSQDSQIQILLFILFLIIYLLTVLGNLLIIVLIFMDSRLHTPMYFFLRNLSFADLCFSTSIVPQVLVHLLVKNKTISFMGCMTQVIVFLLVGCTECALLAVMSYDRYVAVCKPLHYSTIMTQQVCLQLATGSWASGAVVSLVDTTFTFQLPYQGQNIINHYFCEPPALLKLASADTYRTEMAIFAMGVVILLAPVSLILASYWNIISTVIQMQAEEGRLKAFSTCGSHLIVVVLFYGSGIFTYMRPNSKTIKERDKMISVFYTVVTPMLNPIIYSLRNKDVKGALRKLAGRKSFSQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2E9M0</accession>
    <name>A0A4W2E9M0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC113897848</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cell membrane</location>
            <topology evidence="3 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2E9M0"/>
    <dbReference type="STRING" id="30522.A0A4W2E9M0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000046774.1">
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        <property type="gene ID" value="ENSBIXG00000019542.1"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
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        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="41"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="318" mass="34915" checksum="37FCA366FCDC2B2F" modified="2019-09-18" version="1">MEASNQSSVTEFVLLGLSAHPKLEKTFFVLILSMYLVILLGNGVLILVTVSDSHLHTPMYFFLGNLSFLDICYTTSSVPLVLDGFLTPRKTIPFSACAIQMFLSFAMGATECVLLGMMAFDRYMAICNPLRYSIVMRKAVYVSMAASSWLAGGANSLVQISLAVQLPFCGDNVINHFICEILAVLKLACADISINVVSMGVANVIFLGVPVLFIFVSYIFILTTILRIPSVEGRKKAFSTCSAHLTVVVIFYGTILFMYGKPKSKDPLGADKQELADKLISLFYGLLTPMLNPIIYSLRNKDVKAAVKNLMVQKHSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ECA8</accession>
    <name>A0A4W2ECA8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit M</fullName>
            <shortName evidence="5">eIF3m</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">EIF3M</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit M family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027418531.1">
        <property type="nucleotide sequence ID" value="XM_027562730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ECA8"/>
    <dbReference type="SMR" id="A0A4W2ECA8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000020053.1">
        <property type="protein sequence ID" value="ENSBIXP00000033558.1"/>
        <property type="gene ID" value="ENSBIXG00000016348.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005044366.1">
        <property type="protein sequence ID" value="ENSBIXP00005033139.1"/>
        <property type="gene ID" value="ENSBIXG00005013861.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113905436"/>
    <dbReference type="CTD" id="10480"/>
    <dbReference type="GeneTree" id="ENSGT00390000004456"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03012">
        <property type="entry name" value="eIF3m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027528">
        <property type="entry name" value="eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040750">
        <property type="entry name" value="eIF3m_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF2">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18005">
        <property type="entry name" value="eIF3m_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="180"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylserine" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="374" mass="42503" checksum="63736CA2B093D794" modified="2019-09-18" version="1">MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDVESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIPTELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQARVDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQELQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLYDTLNAWKQNLNKVKNSLLSLSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EDS8</accession>
    <name>A0A4W2EDS8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Vascular endothelial growth factor C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Flt4 ligand</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Vascular endothelial growth factor-related protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer; non-covalent and antiparallel. Interacts with FLT4/VEGFR3; the interaction is required for FLT4/VEGFR3 homodimarization and activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EDS8"/>
    <dbReference type="STRING" id="30522.A0A4W2EDS8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000005484.1">
        <property type="protein sequence ID" value="ENSBIXP00000029968.1"/>
        <property type="gene ID" value="ENSBIXG00000011736.1"/>
    </dbReference>
    <dbReference type="OMA" id="ALPQCQA"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043185">
        <property type="term" value="F:vascular endothelial growth factor receptor 3 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000025">
        <property type="entry name" value="vascular endothelial growth factor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004153">
        <property type="entry name" value="CXCXC_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF3">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03128">
        <property type="entry name" value="CXCXC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-0497">Mitogen</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021248273" description="Vascular endothelial growth factor C" evidence="20">
        <location>
            <begin position="18"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="21">
        <location>
            <begin position="117"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000029968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="419" mass="46610" checksum="6093E50F02813EDE" modified="2019-09-18" version="1">MHLLGFCSVACSLLAAALLPGPRRAPAAAAAFESGLGFSDTEPDAGENKAYAGKEMEEQLRSVSSVDELMTVLYPEYWKMYKCQLRKGGWQHSTEQANTNIRTGETLKFAAAHYNTEILRSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKPPCVSVYRCGGCCNSEGQQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRRSLPAALPQCQAANKTCPADYIWNNHVCRCLAQHDFIFSPSAGDDSADGFHDICGPNKELDEETCQCVCKGGLQASSCGPHKELDRDSCQCVCKNKLFPSSCGANREFDENTCQCICKKTCPRNQPLNPGKCACECTENPQKCFLKGKKFQHQTCSCYRRPCTNRVKHCEQGLSFSEEVCRCVPSYWKRPHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EEB6</accession>
    <name>A0A4W2EEB6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-fold modifier 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with UBA5. Interacts with UFC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EEB6"/>
    <dbReference type="STRING" id="30522.A0A4W2EEB6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029362.1">
        <property type="protein sequence ID" value="ENSBIXP00000037572.1"/>
        <property type="gene ID" value="ENSBIXG00000021131.1"/>
    </dbReference>
    <dbReference type="OMA" id="QCNQKIN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005375">
        <property type="entry name" value="UFM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825:SF4">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="62"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="85" mass="9310" checksum="36932CB1BA0665E5" modified="2019-09-18" version="1">MISFKITLTLDPRLPYRVLSVPKNTPFTTVLKFAAEEFKVPLATSAPITNGGTEINPSSPSLAATRDSPSPEMKTQCNQKINKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EF28</accession>
    <name>A0A4W2EF28_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF28"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003831.1">
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        <property type="gene ID" value="ENSBIXG00000001183.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021221871" description="RNA-directed DNA polymerase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="343" mass="38978" checksum="0AF69E0AF75C5EC8" modified="2019-09-18" version="1">MHLTLFFITFYYITIMLLQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYVNCELPDVQVGFRKGREEPEIKLPTPIGSSKEQESSRKTSASLTTPKPLTMWITTNWKILKEIGIPDHLTCLLRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILPPCLFNLYAEYIIRNARLDEAQGGIKIARRNINNLRYKNDPTLMAESEEELKSFLMKVKEESEKVGLKLNIQKTKSMASGPITSWQIDGETMETVADFIFLGSKITADGDCSHEIKSHLLLGRKAITNLDSILKSRDIALPTKVRLVKARVFPVVMYGCESWIMKKAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EF71</accession>
    <name>A0A4W2EF71_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EF71"/>
    <dbReference type="STRING" id="30522.A0A4W2EF71"/>
    <dbReference type="Ensembl" id="ENSBIXT00000003603.1">
        <property type="protein sequence ID" value="ENSBIXP00000030580.1"/>
        <property type="gene ID" value="ENSBIXG00000012616.1"/>
    </dbReference>
    <dbReference type="OMA" id="IWMHSAR"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07429">
        <property type="entry name" value="Cby_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028118">
        <property type="entry name" value="Chibby_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21533">
        <property type="entry name" value="LEUCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21533:SF20">
        <property type="entry name" value="PROTEIN CHIBBY HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14645">
        <property type="entry name" value="Chibby"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000030580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="182" mass="20391" checksum="76D9EBD1969BFFAA" modified="2019-09-18" version="1">MGTARRRVPPDLSVVESGGAEPAHARSGRATPALALAAVDWASGRVYPWEEERLKMPLFGSTFSPKKTPPRKSASLSNLHNLDRSTREVELGLDYGTPTMNLAGQSLKFENGQWIAETGISGGVDRREAQRLRRRNQQLEEENNLLRLKVDILLDMLSETTAESHLMEKELDELKSVSRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EFP4</accession>
    <name>A0A4W2EFP4_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FERM and PDZ domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FRMPD1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Stabilizes membrane-bound GPSM1, and thereby promotes its interaction with GNAI1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="30522.A0A4W2EFP4"/>
    <dbReference type="Ensembl" id="ENSBIXT00000025756.1">
        <property type="protein sequence ID" value="ENSBIXP00000035990.1"/>
        <property type="gene ID" value="ENSBIXG00000019364.1"/>
    </dbReference>
    <dbReference type="OMA" id="ESMDDVC"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13183">
        <property type="entry name" value="FERM_C_FRMPD1_FRMPD3_FRMPD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17168">
        <property type="entry name" value="FERM_F1_FRMPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21942">
        <property type="entry name" value="LGNbd_FRMPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06769">
        <property type="entry name" value="PDZ_FRMPD1_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000203">
        <property type="entry name" value="FERM and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000009">
        <property type="entry name" value="FERM and PDZ domain containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000168">
        <property type="entry name" value="FERM and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000066">
        <property type="entry name" value="FERM and PDZ domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041779">
        <property type="entry name" value="FRMPD1/3/4_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF2">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="57"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="9">
        <location>
            <begin position="181"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="550"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="717"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="790"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="913"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="952"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1066"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1129"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1176"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1232"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="730"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="793"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="961"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="978"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1177"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1233"/>
            <end position="1254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000035990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="1587" mass="173464" checksum="DFE375B67AE48DD2" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EGN6</accession>
    <name>A0A4W2EGN6_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EGN6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000001120.1">
        <property type="protein sequence ID" value="ENSBIXP00000031427.1"/>
        <property type="gene ID" value="ENSBIXG00000013680.1"/>
    </dbReference>
    <dbReference type="OMA" id="CEMVTRM"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="178"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000031427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="449" mass="52595" checksum="2A884A9B39EB00A6" modified="2019-09-18" version="1">MQKSKMAVWEALQIAVKRREAKSKGEKERYKHLNAEFHRIARKDKKAFLRDQCKEIEENNRMGKIRDLFKRIRDTKGIFHAKMGSIKDRNGMDLAEAEDIKKRWQEYTEELYKRDLHDPDNHDGVITDLEPDILKCEVKRALESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYRTIALIPHASKVMLKILQARLQQNVNREIPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLLRNLYAGQETTVRTGHGTTDWFQLGKGVRQVCILSPCLFNFYAEYIMRNAGLEEAQAGIKISRRNMNNLRYADDTTLMAESEEELKSLLMKVKEESEKVGLKLNIQKMKIMVWSHHFMGNRWVNSGNSVRLYFFGLQNHCRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EIG6</accession>
    <name>A0A4W2EIG6_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="3">InaF motif containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">INAFM2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027409318.1">
        <property type="nucleotide sequence ID" value="XM_027553517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EIG6"/>
    <dbReference type="STRING" id="30522.A0A4W2EIG6"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047967.1">
        <property type="protein sequence ID" value="ENSBIXP00000037770.1"/>
        <property type="gene ID" value="ENSBIXG00000021432.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005043322.1">
        <property type="protein sequence ID" value="ENSBIXP00005026850.1"/>
        <property type="gene ID" value="ENSBIXG00005029016.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113900085"/>
    <dbReference type="CTD" id="100505573"/>
    <dbReference type="GeneTree" id="ENSGT00390000005766"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029162">
        <property type="entry name" value="InaF-motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34929:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INAFM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34929">
        <property type="entry name" value="ZGC:153157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15018">
        <property type="entry name" value="InaF-motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="152" mass="15351" checksum="61B768A3A8AF97D0" modified="2019-09-18" version="1">MKERDATPAERGKPATYTGDKKAKMAAKTNKKWVRLATVFAYVLSVSLAAIVLAVYYSLIWQPVGAGTSGGAAGPPPGGSNATGPSGTSGAAAGPNSTGLSRREAPRDAPPLQAARPVSPEPSADSPQAGPLEQPRGAEEDEEEAAAAPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJE5</accession>
    <name>A0A4W2EJE5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3'(2'),5'-bisphosphate nucleotidase 1</fullName>
            <ecNumber evidence="17">3.1.3.57</ecNumber>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">3'-phosphoadenosine 5'-phosphate phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Bisphosphate 3'-nucleotidase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Inositol-polyphosphate 1-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">BPNT1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,3,4-trisphosphate + H2O = 1D-myo-inositol 3,4-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:70319"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:83241"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:70320"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15553"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58282"/>
            <dbReference type="ChEBI" id="CHEBI:58469"/>
            <dbReference type="EC" id="3.1.3.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:15554"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.57" evidence="17"/>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027420270.1">
        <property type="nucleotide sequence ID" value="XM_027564469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJE5"/>
    <dbReference type="SMR" id="A0A4W2EJE5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029250.1">
        <property type="protein sequence ID" value="ENSBIXP00000037487.1"/>
        <property type="gene ID" value="ENSBIXG00000003991.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005055373.1">
        <property type="protein sequence ID" value="ENSBIXP00005027258.1"/>
        <property type="gene ID" value="ENSBIXG00005030846.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113906410"/>
    <dbReference type="CTD" id="10380"/>
    <dbReference type="GeneTree" id="ENSGT00940000157359"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01640">
        <property type="entry name" value="IPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000011">
        <property type="entry name" value="Bisphosphate nucleotidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000006">
        <property type="entry name" value="Bisphosphate nucleotidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF5">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0452">Lithium</keyword>
    <keyword evidence="8 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="20">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="308" mass="33328" checksum="8A9184010B4B893C" modified="2019-09-18" version="1">MASSPTVLMRLVASAYSIAQKAGTIVRRVIAEGDLGIIEKTCATDLQTKADRLVQVSICSSLARKFPKLTIIGEEDLPPEDVDQELIEDGQWEEILKQPCPSQYSAIKEEDLVVWVDPLDGTKEYTEGLLDNVTVLIGIAYEGKAIAGVINQPYYNYQAGPDAVLGRTIWGVLGLGAFGFQLKEAPAGKHIITTTRSHNSQLVTDCITAMNPDDVLRVGGAGNKIIQLIEGKASAYVFASPGCKKWDTCAPEVILHAVGGKLTDIHGNALQYNKEVKHMNSAGVLATLRNYDYYASRVPQSVKNALVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EJI3</accession>
    <name>A0A4W2EJI3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EJI3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000044795.1">
        <property type="protein sequence ID" value="ENSBIXP00000033401.1"/>
        <property type="gene ID" value="ENSBIXG00000016187.1"/>
    </dbReference>
    <dbReference type="OMA" id="CSTINMV"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="117"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000033401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="466" mass="53864" checksum="8E1AD30F5F4F3804" modified="2019-09-18" version="1">MGKTRDLFKKIRDTKGTFHAKMGSIKDRNGMDLTETEDIKKRWQEYTEELYKKDLKDQDNHDGVITHLEPDILECEVKWALESFTMNKASGGDGISVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHTIALISHASKVMLKILQARLQQYVTHELPDVQTGFRKGRGTRDQIANIRWVIEKAREFQKNIYFCFIDYAKAFDCVDHNKLWKILKEMGIPDHLTCLSRNLYAGQEATVRTGHGTTDWFQIGKGVRQGCILSPCLFNLYAEYIMRNAGLEEAQAGIKISGRNINNLRYADDTTLMAENEELKSLLMKMKEESKKVGLKLNIRKTKIMASGPITSWQIDGETVETVADLIFLGSKITTDGDCSHEIKRRLLLGRKVMTNLDSVLKSRDITLPTKVRLVKAMVFPVVMYGWQSWTIKKAERQRIDAFELWCWRRLLRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EK33</accession>
    <name>A0A4W2EK33_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL42</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL42 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EK33"/>
    <dbReference type="STRING" id="30522.A0A4W2EK33"/>
    <dbReference type="Ensembl" id="ENSBIXT00000045352.1">
        <property type="protein sequence ID" value="ENSBIXP00000034120.1"/>
        <property type="gene ID" value="ENSBIXG00000017064.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019346">
        <property type="entry name" value="Ribosomal_mL42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13450:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13450">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10210">
        <property type="entry name" value="MRP-S32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5021294659" description="Large ribosomal subunit protein mL42" evidence="9">
        <location>
            <begin position="21"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000034120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="192" mass="22131" checksum="FA907464F4CC3364" modified="2019-09-18" version="1">MPSNHLILCCPLLLLPSIFPSIRVFSDKSALCIRWPRYWSFSISPFKEYAGLISFRMDWFDLLAVQGTLESCPAAQFESNSLPLSLYMVQFSHPYMTTGKTIVLTVWTLAVHPSVDIPHKHTKPIPRPDPVHDDEETHDAVLKTRSEERSEHSVPGPTIERLGEMFFSTKHHRNPRGQNRRRCKKLNPPEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELM5</accession>
    <name>A0A4W2ELM5_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CDH4</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELM5"/>
    <dbReference type="STRING" id="30522.A0A4W2ELM5"/>
    <dbReference type="Ensembl" id="ENSBIXT00000047355.1">
        <property type="protein sequence ID" value="ENSBIXP00000036928.1"/>
        <property type="gene ID" value="ENSBIXG00000000374.1"/>
    </dbReference>
    <dbReference type="OMA" id="QICERPG"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000027">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000045">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014868">
        <property type="entry name" value="Cadherin_pro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF81">
        <property type="entry name" value="CADHERIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08758">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01820">
        <property type="entry name" value="DESMOCOLLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="94"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="202"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="317"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="432"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="538"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="50"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="794"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="796"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000036928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="840" mass="92320" checksum="F5E0FC55D0009B61" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ELZ8</accession>
    <name>A0A4W2ELZ8_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="13">PPIase</shortName>
            <ecNumber evidence="13">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PPIL4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 13">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027407603.1">
        <property type="nucleotide sequence ID" value="XM_027551802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ELZ8"/>
    <dbReference type="SMR" id="A0A4W2ELZ8"/>
    <dbReference type="STRING" id="30522.A0A4W2ELZ8"/>
    <dbReference type="Ensembl" id="ENSBIXT00000029506.1">
        <property type="protein sequence ID" value="ENSBIXP00000037600.1"/>
        <property type="gene ID" value="ENSBIXG00000021192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113898532"/>
    <dbReference type="CTD" id="85313"/>
    <dbReference type="OMA" id="APKCCEN"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01921">
        <property type="entry name" value="cyclophilin_RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12235">
        <property type="entry name" value="RRM_PPIL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000016">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000267">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035542">
        <property type="entry name" value="CRIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035538">
        <property type="entry name" value="Cyclophilin_PPIL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9 13" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="15">
        <location>
            <begin position="6"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="240"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="368"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="423"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000037600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="492" mass="57122" checksum="FACDCFD47D39B510" modified="2019-09-18" version="1">MAVLLETTLGDVVIDLYTEERPRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTGDPTGTGRGGESVFGQLYGDQASFFEAEKVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDGVHTVFGEVTEGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLLIPDRSPEPTREQLDSGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQAILLEMVGDLPDADIKPPENVLFVCKLNPVTTDEDLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIEFEKEEDCEKAFFKMDNVLIDDRRIHVDFSQSVAKVKWKGKGGKYTKSDFKEYEKEQDKPSNLVLKDKVKPKQDAKYELVLDEQAEDSKAGHSHTSKKHKKKTRHCSEEKEDEDYMPIKNTNQDIYREMGFGHYEEEESCWEKQKSEKRDRPQNRSRSRSRERDGHYSNSHKSKYQADPYERERSKKRDRSRSPRKSKDKEKSKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ESB9</accession>
    <name>A0A4W2ESB9_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9 10">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2ESB9"/>
    <dbReference type="STRING" id="30522.A0A4W2ESB9"/>
    <dbReference type="Ensembl" id="ENSBIXT00000034813.1">
        <property type="protein sequence ID" value="ENSBIXP00000039901.1"/>
        <property type="gene ID" value="ENSBIXG00000023916.1"/>
    </dbReference>
    <dbReference type="OMA" id="FAVHRWH"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="421"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="552"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000039901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="583" mass="63482" checksum="112B74CDB4F01A5E" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2ETL1</accession>
    <name>A0A4W2ETL1_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="5">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SCAMP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCAMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408732.1">
        <property type="nucleotide sequence ID" value="XM_027552931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2ETL1"/>
    <dbReference type="SMR" id="A0A4W2ETL1"/>
    <dbReference type="Ensembl" id="ENSBIXT00000049865.1">
        <property type="protein sequence ID" value="ENSBIXP00000040330.1"/>
        <property type="gene ID" value="ENSBIXG00000024382.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005010677.1">
        <property type="protein sequence ID" value="ENSBIXP00005029487.1"/>
        <property type="gene ID" value="ENSBIXG00005009438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899709"/>
    <dbReference type="CTD" id="9522"/>
    <dbReference type="GeneTree" id="ENSGT00940000157310"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000429181">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF8">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000040330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
        </source>
    </evidence>
    <sequence length="338" mass="37974" checksum="75F871A861094EB4" modified="2019-09-18" version="1">MSDFDSNPFADPDLNNPFKDPSVTQVTRNVPPGLDEYNPFSDSRTPPPGNVKMPNVPSTQPAIMKPTEEHPAYTQIAKEHALAQAELLKRQEELERKAAELDRREREMQNLSQHGRKNNWPPLPDNFPVGPCFYQDFSVDIPVEFQKTVKIMYYLWMFHAVTLFLNIFGCLAWFCVDPPRGVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFHNWGNCGWISSLTGLNKSIPVGIMMIIIAALFTASAVISLVMFKKVHGLYRTTGASFEKAQQEFATGVMSNKTVQTAAANAASTAASSAAQNAFKGNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EVP0</accession>
    <name>A0A4W2EVP0_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ADP-ribosylation factor 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ARL14</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with ARL14EP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027408183.1">
        <property type="nucleotide sequence ID" value="XM_027552382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4W2EVP0"/>
    <dbReference type="SMR" id="A0A4W2EVP0"/>
    <dbReference type="STRING" id="30522.A0A4W2EVP0"/>
    <dbReference type="Ensembl" id="ENSBIXT00000050472.1">
        <property type="protein sequence ID" value="ENSBIXP00000041100.1"/>
        <property type="gene ID" value="ENSBIXG00000025278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSBIXT00005001210.1">
        <property type="protein sequence ID" value="ENSBIXP00005008009.1"/>
        <property type="gene ID" value="ENSBIXG00005013146.1"/>
    </dbReference>
    <dbReference type="GeneID" id="113899088"/>
    <dbReference type="CTD" id="80117"/>
    <dbReference type="GeneTree" id="ENSGT00940000162138"/>
    <dbReference type="OMA" id="AQIIMLG"/>
    <dbReference type="Proteomes" id="UP000314981">
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        <location>
            <begin position="20"/>
            <end position="27"/>
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        <ligand>
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        <location>
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        <ligand>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
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        <source>
            <dbReference type="Proteomes" id="UP000429181"/>
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    <sequence length="192" mass="21490" checksum="9A07AAD6CFD40EED" modified="2019-09-18" version="1">MGLLNSKKPKAKQARILLLGLDSAGKSTLLYKLKLDKDIVTIPTVGFNVEMIELAKGVSLTVWDVGGQEKMRATWGLYCENTDGLVYVVDSTDTQRLEDSRKEFEHILKNEYIKNVPVVLLANKQDMPGALSAEDITRMFKVKQLCSDRNWCVQPCCAVTGDGLMEGFQKLTGFVKSHMKSRGDTLAFFKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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            <begin position="117"/>
            <end position="350"/>
        </location>
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            <dbReference type="PROSITE" id="PS50878"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="365" mass="42189" checksum="509353F325958AEE" modified="2019-09-18" version="1">MGKTRDLFKKIRDTQGTFHAKMGSIKDRNGMDLTEAEDIKKRWQEYTEELYKKDLHDPDSHDGVITHLEPDSLECEVKWNLESITTNKASGGDGIPVELFQILKDDAVKVLHSICQQIWKTQQWPQDWKRSVFIPIPKKGNAKECSNYHKIVLISYASKVMLKILQARLQQYVNHELPDVQAGFRKGRGTRDQIANICWIMEKRREFQKNIYFCFTDYAKAFDCVDHNKLWKISKEMGIPDHLIFLLRNLYAGQEATVRTGHGTTTDWFIMRNAGLEEAQAGIKIAGRNINNLRYADDTTLMSESEEELKSLLMKVKKESEKDGLKLNIQKTKIMASGPITSWEIDGETVADFSFWAPNHCRWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZ08</accession>
    <name>A0A4W2EZ08_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polyamine modulated factor 1</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
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            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZ08"/>
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        <property type="gene ID" value="ENSBIXG00000001438.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007128">
        <property type="entry name" value="PMF1/Nnf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15459">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15459:SF3">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03980">
        <property type="entry name" value="Nnf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23531" checksum="05CB40DB798AAD53" modified="2019-09-18" version="1">MAEASSVNVGSGCEEKGPEELSQEPARPGTNISRVKLFDTMVDTFLQKLVAAGSFQRFTDCYKRFYQLQPEMTQRIYDKFVTQLQTSIQEEISEIKAEGNLEAVLIALDAIVEESKDRKEQAWRPSGIPEKDLRSAMAPYLLQQRDALQRRVQRQEAENRQLADAVLAGRRQLEELQLQAQARQQAWQALHREQKELLAVLKEPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2EZ32</accession>
    <name>A0A4W2EZ32_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2EZ32"/>
    <dbReference type="Ensembl" id="ENSBIXT00000040096.1">
        <property type="protein sequence ID" value="ENSBIXP00000042332.1"/>
        <property type="gene ID" value="ENSBIXG00000001476.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="384" mass="43931" checksum="AEFEAAAE559A8EBF" modified="2019-09-18" version="1">MNAKECSNYRTIALISLASKVMLKILQARLQQYVNRELPDVQAGFRKGRGTRDQIANIRWIMEKAREFQKNIYFCFIDYAKAFDCGDHNKLWKILKEMGIPDHLTCLLRNLYAGQEATVRTGYGTTDWFQIGKGVCQGCILSPCLFNLYADYIMKNAGLEETQAGIKIAGRNINNLRYADDTTLMAESEEELKSLLMKVKEEREKVGLKLNIQKTKIMASGPIISWEIDGETVETVSGFIFLGSKITADGDCSHEIKRRLLLGRKVMTNLDSILKSRNITLPTKVHLVKAMVFPVVMYGCESWTVKKAERQRIDGFELWCWRRLLRVPWTARRSNQSILKEISPGISLEGMMLKLKLQYFGYLMQRVDSLEKTLMLGGIGGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F0S7</accession>
    <name>A0A4W2F0S7_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="17 19">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="4 19">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Component of the CMG helicase complex, a hexameric ring of related MCM2-7 subunits stabilized by CDC45 and the tetrameric GINS complex. May interact with MCM10. Interacts with TIPIN. Interacts with CDT1. Interacts with MCMBP. Interacts with DDI2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A4W2F0S7"/>
    <dbReference type="STRING" id="30522.A0A4W2F0S7"/>
    <dbReference type="Ensembl" id="ENSBIXT00000051862.1">
        <property type="protein sequence ID" value="ENSBIXP00000042983.1"/>
        <property type="gene ID" value="ENSBIXG00000027018.1"/>
    </dbReference>
    <dbReference type="OMA" id="RHQQTDK"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000001">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000091">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000004">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF73">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 19" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="8 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="21">
        <location>
            <begin position="346"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="676"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000042983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="821" mass="92846" checksum="EE92A288FEBBD9AB" modified="2019-09-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F112</accession>
    <name>A0A4W2F112_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgin subfamily A member 7/ERF4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F112"/>
    <dbReference type="STRING" id="30522.A0A4W2F112"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041411.1">
        <property type="protein sequence ID" value="ENSBIXP00000043054.1"/>
        <property type="gene ID" value="ENSBIXG00000027107.1"/>
    </dbReference>
    <dbReference type="OMA" id="MEYICLI"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005798">
        <property type="term" value="C:Golgi-associated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039735">
        <property type="entry name" value="CHIC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13005">
        <property type="entry name" value="CYSTEINE-RICH HYDROPHOBIC DOMAIN PROTEIN BRAIN X-LINKED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13005:SF3">
        <property type="entry name" value="CYSTEINE-RICH HYDROPHOBIC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="4">
        <location>
            <begin position="45"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="165" mass="19253" checksum="AB45A9C08074E05B" modified="2019-09-18" version="1">MADFDEIYEEEEDEERALEEQLLKYSPDPVVVRGSGHVTVFGLSNKFESEFPSSLTGKVAPEEFKASINRVNSCLKKNLPVIVRWLLCGCLCCCCTLGCSMWPVICLSKRTRRSIEKLLEWENNRLYHKLCLHWRLSKRKCETNNMMEYVILIEFLPKTPIFRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F1J2</accession>
    <name>A0A4W2F1J2_BOBOX</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat TRM82 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F1J2"/>
    <dbReference type="SMR" id="A0A4W2F1J2"/>
    <dbReference type="STRING" id="30522.A0A4W2F1J2"/>
    <dbReference type="Ensembl" id="ENSBIXT00000041858.1">
        <property type="protein sequence ID" value="ENSBIXP00000043281.1"/>
        <property type="gene ID" value="ENSBIXG00000005745.1"/>
    </dbReference>
    <dbReference type="OMA" id="DCIPVVY"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106004">
        <property type="term" value="P:tRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000454">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03056">
        <property type="entry name" value="TRM82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028884">
        <property type="entry name" value="Trm82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288:SF0">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288">
        <property type="entry name" value="WD40 REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 9 10" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="377"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000314981"/>
        </source>
    </evidence>
    <sequence length="414" mass="45858" checksum="938C8AA264982F5D" modified="2019-09-18" version="1">MASSAGLALCGHALVVRGGSRLLATSTSSSDGDSLFIYDCSAAEKKSQENKGEDGQPVDQGSDTVLASTFSKSGSYFVLTDDSKRLILFRTNPWQCLSVRTVVRRCTALTFTASEEKILVADKSGDVYSFSVLEPHGGGRLELGHLSMLLDVAVSPDDRFVLTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRIFVVPNHPELLLSSSGDCTLRLWEYRSGRELHCCPLTSLQEPTEPWSDKRFAVSRITYWSQEDCVALSCDGLPVVYIFQLDAAQRQLVPRQLLTFQHRVWDAAFEEGHGLWVLQDCREDPLVLYRPVGGQWQSAPESAELRRVCAHVRVNWAMLEGCAGVDSGFSSLYKATCDNMTTYLKKKEERLQQQLEKKRRQAPPPGPNGPTKKMRAGELAQGCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-09-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4W2F3I3</accession>
    <name>A0A4W2F3I3_BOBOX</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Bos indicus x Bos taurus</name>
        <name type="common">Hybrid cattle</name>
        <dbReference type="NCBI Taxonomy" id="30522"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Artiodactyla</taxon>
            <taxon>Ruminantia</taxon>
            <taxon>Pecora</taxon>
            <taxon>Bovidae</taxon>
            <taxon>Bovinae</taxon>
            <taxon>Bos</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-11" db="EMBL/GenBank/DDBJ databases">
            <title>Haplotype-resolved cattle genomes.</title>
            <authorList>
                <person name="Low W.Y."/>
                <person name="Tearle R."/>
                <person name="Bickhart D.M."/>
                <person name="Rosen B.D."/>
                <person name="Koren S."/>
                <person name="Rhie A."/>
                <person name="Hiendleder S."/>
                <person name="Phillippy A.M."/>
                <person name="Smith T.P.L."/>
                <person name="Williams J.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A4W2F3I3"/>
    <dbReference type="Ensembl" id="ENSBIXT00000052339.1">
        <property type="protein sequence ID" value="ENSBIXP00000043580.1"/>
        <property type="gene ID" value="ENSBIXG00000027730.1"/>
    </dbReference>
    <dbReference type="OMA" id="TEPVHIH"/>
    <dbReference type="Proteomes" id="UP000314981">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSBIXP00000043580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:000